Methyltransferases saccharomyces cerevisiae proteins. Medical search. Frequent questions

In addition, yeast Nep1 binds to a 6-nt RNA-binding motif also found in 18S rRNA and facilitates the incorporation of ribosomal protein Rps19 during the formation of pre-ribosomes. (rcsb.org)
These same principles of mechanistic enzymology, in combination with the powerful approaches of yeast genetics and molecular biology, also allow the lab to investigate the biological function of enzymes in living cells, including a tRNA splicing enzyme and a tRNA methyltransferase whose biological roles in the yeast Saccharomyces cerevisiae are well-defined, but whose possible role(s) in other organisms remain a mystery. (osu.edu)
Initial characterization of Trm10 yielded the surprising result that up to three different Trm10 homologs exist simultaneously in several metazoans, including mouse and humans, while in yeast, the single Trm10 protein catalyzes all known m1G9 modifications. (osu.edu)
A yeast assay to discriminate between the antiviral proteins IFIT1 and IFIT1B. (elifesciences.org)
Using both yeast and mammalian cells as model organisms, my lab is focused on understanding the biological functions of protein arginine methylation at the molecular level using cell biological, biochemistry, proteomics, and genomics approaches. (buffalo.edu)
In addition, we identified proteins that interact with the KRAB domain of PRDM9 in yeast two-hybrid assay screens, particularly CXXC1, a member of the COMPASS complex. (springer.com)
One pathway, which has been extensively studied in yeast, is mainly guided by chromatin structure and the other, analyzed in detail in mice, is driven by the sequence-specific DNA-binding PR domain-containing protein 9 (PRDM9). (springer.com)
To explore the scope of RBPs across eukaryotic evolution, we determined the in vivo RBP repertoire of the yeast Saccharomyces cerevisiae and identified 678 RBPs from yeast and additionally 729 RBPs from human hepatocytic HuH-7 cells. (nature.com)
Only 60% of yeast and 73% of the human RBPs have functions assigned to RNA biology or structural motifs known to convey RNA binding, and many intensively studied proteins surprisingly emerge as RBPs (termed 'enigmRBPs'), including almost all glycolytic enzymes, pointing to emerging connections between gene regulation and metabolism. (nature.com)
c ) Overlap of mRNA interactome proteins in yeast and HuH-7. (nature.com)
d ) Validation of the yeast mRNA interactome using western blotting of input samples and eluate after interactome capture with specific antibodies (ADH1, alcohol dehydrogenase 1, PUB1) or against TAP-tagged proteins (PGK1, phosphoglycerate kinase 1, TDH1, triose phosphate dehydrogenase, TRX2, thioredoxine 2, SHE2, Swi5p-dependent HO Expression 2). (nature.com)
Last, through studying in vitro methylation of yeast lysates using recombinantly expressed enzymes, we identify putative yeast methyltransferase Ynl092wp as a protein histidine N-methyltransferase that binds its substrates using a sequential mechanism and predict that Ykl162cp is an RNA methyltransferase. (ubc.ca)
Nop1p / Fibrillarin was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). (thermofisher.com)
The Nop1p protein is essential for yeast viability and is localized in the nucleoli. (thermofisher.com)
Consistent with these results, proteins subject to enhanced ribosome pausing in yeast lacking mcm5U and mcm5s2U are more likely to be down-regulated and contain a larger number of AGA/GAA clusters. (genesilico.pl)

Here we use quantitative proteomics to show a direct link between wobble uridine 5-methoxycarbonylmethyl (mcm5) and 5-methoxy-carbonyl-methyl-2-thio (mcm5s2) modifications catalyzed by tRNA methyltransferase 9 (Trm9) in tRNAArg(UCU) and tRNAGlu(UUC) and selective translation of proteins from genes enriched with their cognate codons. (genesilico.pl)

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C . (yeastgenome.org)

When a virus is detected, a signaling protein called interferon is released and it induces an antiviral state by upregulating a whole slew of interferon-stimulated genes. (elifesciences.org)
IFIT genes are among the most highly upregulated of these genes, and IFIT proteins target a wide range of viruses ( Diamond and Farzan, 2013 ). (elifesciences.org)
Genome-wide studies in Saccharomyces cerevisiae , for example, have revealed that promoters of most actively transcribed genes are depleted of nucleosomes and that histone density is inversely proportional to the transcription rate within coding regions, suggesting that nucleosomes are dynamically disassembled and reassembled at each passage of the polymerase ( 16 ). (asm.org)
Controlling for bias in protein expression and alternations in mRNA expression, we find that loss of Trm9 selectively impairs expression of proteins from genes enriched with AGA and GAA codons under both normal and stress conditions. (genesilico.pl)

These small changes alter the target residue site specificity for methylation and allow the SET domain methyltransferases to target many different residues. (wikipedia.org)
There are three different types of protein arginine methyltransferases (PRMTs) and three types of methylation that can occur at arginine residues on histone tails. (wikipedia.org)
Protein methylation is one of the major post-translational modifications (PTMs) in the cell. (edu.au)
Recently, protein arginine methylation has emerged as a major regulator of protein function. (buffalo.edu)
In the metazoans, protein arginine methylation has been shown to play a role in the differentiation and development as well as etiology of human diseases such as multiple sclerosis, spinal muscular atrophy, and cancer. (buffalo.edu)
Many spliceosomal and associated proteins are subject to regulation via post-translational modifications such as methylation and phosphorylation. (buffalo.edu)
Previous work from my lab has established a critical role for protein arginine methylation in pre-mRNA splicing, wherein it functions by an as yet poorly understood mechanism to control co-transcriptional recruitment of spliceosomal and associated proteins to nascent pre-mRNA molecules. (buffalo.edu)
Cytosine C5 methylation is an important epigenetic control mechanism in a wide array of eukaryotic organisms and generally carried out by proteins of the C-5 DNA methyltransferase family (DNMTs). (mdpi.com)

Positional cloning of the RID2 gene revealed that it encodes an evolutionarily conserved methyltransferase-like protein, which was found to localize in the nucleus, with accumulation being most evident in the nucleolus. (nih.gov)
Histones are evolutionarily conserved DNA-binding proteins. (aging-us.com)

For instance, the Type I protein arginine methyltransferases are known to methylate a number of proteins that contain an arginine glycine glycine (RGG)-motif [6]. (deepdyve.com)

The purpose of this project was to examine how PRMTs bind to and methylate their substrates, and explore their roles in the stress response using Saccharomyces cerevisiae as a model organism. (ubc.ca)
Recently is has been demonstrated that A. fumigatus possesses a novel thiomethyltransferase protein called GtmA that has the ability to thiomethylate GT in vivo, which aids the organism in regulating GT biosynthesis. (maynoothuniversity.ie)
In this work we have engineered Saccharomyces cerevisiae, a GT-naïve organism, to express A. fumigatus GtmA. (maynoothuniversity.ie)

This suggests that human IFIT1 protein recognizes viral mRNA by a different and as-yet-unknown means. (elifesciences.org)
Proteins shown in red (FDR 0.01) represent the mRNA interactome. (nature.com)
protein_coding" "Cz03g26100.t1","No alias","Chromochloris zofingiensis","Pre-mRNA polyadenylation factor Fip1 domain [Interproscan]. (ntu.edu.sg)
Members of this family include the signal-transducing G protein beta subunit, as well as other proteins that regulate signal transduction, transcription, pre-mRNA splicing, cytoskeletal organization, and vesicular fusion. (embl.de)

The highly conserved eukaryotic nucleolar protein Nep1 has an essential but unknown function in 18S rRNA processing and ribosome biogenesis. (rcsb.org)
The histone H2A.F/Z (H2AZ) variant is a functionally distinct, highly conserved histone subgroup that likely represents a separate evolutionary lineage of histone H2A proteins. (sdbonline.org)
The family of WD-repeat proteins comprises over 30 different proteins that share a highly conserved repeating motif [Neer, E. J., Schmidt, C. J., Nambudripad, R., & Smith, T. F. (1994) Nature 371, 297-300]. (embl.de)
Throughout evolution, eukaryotes adopted a network of highly conserved proteins that buffer the positive charges of histones, maintaining their solubility and, therefore, avoiding aberrant interactions with other cellular components. (aging-us.com)

Histone methyltransferases (HMT) are histone-modifying enzymes (e.g., histone-lysine N-methyltransferases and histone-arginine N-methyltransferases), that catalyze the transfer of one, two, or three methyl groups to lysine and arginine residues of histone proteins. (wikipedia.org)
Consequently, amino acid variations through changing the type of residues of the target sites or key flanking residues could directly or indirectly influence PTM of protein and bring about a detrimental effect on protein function. (deepdyve.com)

Instead of SET, non-SET domain-containing histone methyltransferase utilizes the enzyme Dot1. (wikipedia.org)
2003). Trm10, like Thg1, is a member of a previously undescribed family of proteins with no identifiable homology to any other enzyme family, including other S-adenosyl-methionine (SAM)-dependent methyltransferases. (osu.edu)
However, many viruses have evolved ways to fly under the radar either by "snatching" cap1 structures from host mRNAs, or by encoding a methyltransferase enzyme that allows them to make their own cap1 structures ( Hyde and Diamond, 2015 ). (elifesciences.org)
Although viewed as a constitutive housekeeping enzyme in the past, PP2A is a highly regulated phosphatase and is emerging as an important regulator of multiple cellular processes involving protein phosphorylation. (ac.be)
2 Protein phosphatase 2A (PP2A) is a very abundant - it accounts for as much as 1% of total cellular proteins - ubiquitous and remarkably conserved enzyme. (ac.be)
TrmD (tRNA-(N(1)G37) methyltransferase) is an essential tRNA modification enzyme in bacteria that prevents +1 errors in the reading frame during protein translation and represents an attractive potential target for the development of new antibiotics. (bvsalud.org)
Here we performed a structure-based design of inhibitors of tRNA-(N1G37) methyltransferase, TrmD, which is an essential enzyme in many bacterial pathogens. (bvsalud.org)

Recent work constructing protein interaction networks in Saccharomyces cerevisiae suggested the methyltransferase Bud23 was involved in ribosome biogenesis (1). (utexas.edu)
Capture Compounds are trifunctional small molecules to reduce the complexity of the proteome by functional reversible small molecule-protein interaction followed by photo-crosslinking and purification. (jove.com)
PRDM9 is a member of the PRDM family of transcription regulators, but unlike other family members, it contains a Krüppel-associated box (KRAB)-related domain that is predicted to be a potential protein interaction domain. (springer.com)
In S. cerevisiae , this interaction is at least in part provided by Spp1 that directly interacts with both methylated H3K4 near DSB sites and the axis-localized protein Mer2 (Acquaviva et al. (springer.com)
Recent findings relating to the function of the human uracil-5 methyltransferase (U5MT), TRMT2A, and its interaction with 5-FU metabolites incorporated within tRNAs, lead to an additional hypothesis that is proposed here. (bvsalud.org)
We generated a global genetic interaction network for Saccharomyces cerevisiae, constructing more than 23 million double mutants, identifying about 550,000 negative and about 350,000 positive genetic interactions. (thebiogrid.org)
PTM of proteins constitutes a highly diverse and dynamic regulatory layer affecting all aspects of a protein from protein folding, localization, interaction and bioactivity to its stability and ultimately www.selleckchem.com/products/MLN-2238.html degradation. (cd177signaling.com)

All ribosomes are made from a large and small subunit that in turn are composed of protein and RNA. (utexas.edu)
Bud23 is a SAM methyltransferase important for the proper biogenesis of the small ribosomal subunit. (utexas.edu)
Protein Phosphatase 2A (PP2A) is a widely expressed family of protein phosphatases made of a core dimer, composed of a catalytic (C) subunit and a structural (A) subunit, in association with a third variable regulatory (B) subunit. (ac.be)
protein_coding" "Cz02g32120.t1","No alias","Chromochloris zofingiensis","B-block binding subunit of TFIIIC [Interproscan]. (ntu.edu.sg)
Folding of proteins with WD-repeats: comparison of six members of the WD-repeat superfamily to the G protein beta subunit. (embl.de)

We show that this conserved RNA interactome harbours many proteins without previously assigned roles in RNA biology (enigmRBPs), including surprisingly many metabolic enzymes. (nature.com)
Protein arginine methyltransferase 5 (PRMT5) is an important type II arginine methyltransferase that can play roles in cancers in a highly tissue-specific manner, but its role in the carcinogenesis and metastasis of head and neck squamous cell carcinoma (HNSCC) remains unclear. (bvsalud.org)
We are interested in understanding at the molecular level how recombination occurs and what roles are played by the many proteins involved in DNA recombination, repair and replication. (brandeis.edu)
We are interested in determining what are the specific biochemical roles played by the many proteins implicated in DNA recombination, repair and replication. (brandeis.edu)
Silencing at mating locus HMR requires silencers, and one of the roles of the silencer is to recruit Sir proteins. (sdbonline.org)
Plastid-specific ribosomal proteins (PSRPs) have been proposed to play roles in the light-dependent regulation of chloroplast translation. (cipsm.de)

Specific modifications commonly found at the junction of the stems of the cloverleaf structure (Figure 1), referred to as the core, elbow or hinge in the three-dimensional structure of most tRNAs, are often associated with functional folding, allowing tRNAs to fold into the common L-shape form critical to ribosomal protein synthesis. (datexis.com)
Here we demonstrate that PSRP1 is not a bona fide ribosomal protein, but rather a functional homologue of the Escherichia coli cold-shock protein pY. (cipsm.de)

The class of lysine-specific histone methyltransferases is subdivided into SET domain-containing and non-SET domain-containing. (wikipedia.org)
2010 ). PRDM9 includes several functional domains, such as a DNA-binding zinc finger array, a histone lysine methyltransferase PR/SET (PRDI-BF1 and RIZ1 homology) domain, and a Krüppel-associated box (KRAB)-related domain. (springer.com)

4 While proteins can be phosphorylated on nine amino acids, serine, threonine and tyrosine phosphorylation are by far the most predominant in eukaryotic cells. (ac.be)
posttranslational modifications, amino acid variations, computational mutation analysis, protein PTM predictor, network biology Introduction Protein PTMs are biochemical alterations of amino acids that change the physicochemical properties of target proteins, leading to structural changes and therefore regulating protein-protein interactions and cellular signal transduction in developmental and cancer pathways [1]. (deepdyve.com)

Along with PTMs, the partial or complete disassembly of nucleosomes allows the exchange and degradation of pre-existing histone proteins, with the incorporation of newly synthesised histones onto chromatin that can eventually result in the resetting of previous epigenetic marks. (aging-us.com)

Protein arginine N-methyltransferases (PRMTs) catalyze the transfer of methyl groups from the methyl donor S-adenosyl-L-methionine (SAM) to polypeptide substrates. (ubc.ca)

We are investigating how Pbx and Meis proteins contribute to the specificity of Hox function in development and how disruption of their activities leads to neoplasia. (stanford.edu)
The specificity of the proteins is determined by the sequences outside the repeats themselves. (embl.de)

Here, we investigated six overexpressed S. cerevisiae protein MTases (Rkm1, Rkm4, Efm4, Efm7, Set5 and Hmt1) to identify PTMs of potential functional relevance. (edu.au)
To increase the utilization of current computational resources, we 﫿rst provide an overview of computational prediction of amino acid variations that influence protein PTMs and their functional analysis. (deepdyve.com)
The development of better methods for mutation analysis-related protein PTMs will help to facilitate the development of personalized precision medicine. (deepdyve.com)
Most PTMs are catalyzed by highly specific protein modifying enzymes, which have some specific recognition motif. (deepdyve.com)
7] analyzed amino acid variations of 15 different PTMs and indicated that about 4.5% of amino acid variations may affect protein function through disruption of PTMs, and the mutation of 238 PTMs sites in human proteins was causative of disease. (deepdyve.com)
In this regard, comprehensive studies of the impact of amino acid variation on protein PTMs will be helpful for further understanding of how genetic polymorphisms are involved in regulating biological and pathological processes and providing instructive information for drug development of various related diseases. (deepdyve.com)
Recent advances in the field of protein post-translational modifications (PTMs) have uncovered their widespread occurrence and physiological relevance. (cd177signaling.com)
In the absence of functional annotation of proteins from PTMs many key functions of bioactive proteins will be opaque and hence hypotheses based on traditional shotgun analyses, may be misleading or even worse, totally wrong. (cd177signaling.com)

In Saccharomyces cerevisiae, over 20 protein methyltransferases (MTases) and their respective substrates have been identified. (edu.au)

This suggests the participation of Nep1 in a methyltransferase reaction during ribosome biogenesis. (rcsb.org)

Although intracellular signal transduction is often portrayed as a protein kinase 'domino effect', the counterbalancing function of phosphatases, and thus the control of phosphatase activity, is equally relevant to proper regulation of cellular function. (ac.be)
WD-repeat proteins are a large family found in all eukaryotes and are implicated in a variety of functions ranging from signal transduction and transcription regulation to cell cycle control and apoptosis. (embl.de)

S. cerevisiae ) (BY4741) and of human hepatocytic cells (HuH-7). (nature.com)

As CXXC1 is orthologous to Saccharomyces cerevisiae Spp1 that links DSB sites to the DSB machinery on the chromosome axis, we propose that these molecular interactions involved in the regulation of meiotic DSB formation are conserved in mouse meiosis. (springer.com)
These glycoproteins include the CD98 heavy chain protein of Mus musculus (gbU25708) and the orthologous 4F2 cell surface antigen heavy chain of Homo sapiens (spP08195). (tcdb.org)

Here we use a Capture Compound with S -adenosyl- L -homocysteine-binding as selectivity function to isolate methyltransferases from an Escherichia coli whole cell lysate and identify them by MS. (jove.com)

b ) Volcano plot showing the distribution of proteins according to their enrichment in crosslinked (CL) over non-CL samples. (nature.com)
Taxonomic distribution of proteins containing WD40 domain. (embl.de)

Ribosomes are the cellular structures responsible for the synthesis of protein in all branches of life. (utexas.edu)
Within the cell, RNA polymerase III (Pol III) is responsible for the production of small, untranslated structural RNAs for protein synthesis. (buffalo.edu)
Regulating the biogenesis of these small RNAs is important, as the availability of components of the protein synthesis apparatus is a determinant of a cell's biosynthesis capacity and must be produced in high quantity to fulfill the cell's biosynthetic demand during growth. (buffalo.edu)
Translational frameshift errors are often deleterious to the synthesis of functional proteins and could therefore be promoted therapeutically to kill bacteria. (bvsalud.org)
We have identified the proteins necessary to carry out the initial steps in strand invasion and the beginning of new DNA synthesis, which is significantly different from the normal process of replication. (brandeis.edu)

DSB sites are preferentially located within chromatin loops, while several proteins that are required for DSB formation (Rec114, Mei4, and Mer2) localize on the chromosome axis (Blat et al. (springer.com)
We have shown that the invasion of DNA strands into a donor template region requires the action of the chromatin remodeling protein Rad54 that enables the recombination machinery to gain access to "closed" regions of DNA. (brandeis.edu)
Molecular analysis suggested that Esc2p contributed to silencing in a manner similar to Sir1p and probably helped recruit or stabilize the other Sir proteins, while Htz1p present at HMR assembled a specialized chromatin structure necessary for silencing (Dhillon, 2000). (sdbonline.org)
These dedicated proteins are generally known as histone chaperones and effectively control histone supply and chromatin dynamics [ 8 ]. (aging-us.com)

A suppressor of the trm6-504 mutation in the tRNA m1A58 methyltransferase (Trm6p/Trm61p), which causes a reduced level of tRNAi Met, was mapped to MTR4. (marquette.edu)

Therefore, our structures together with previous genetic data suggest that Nep1 is a genuine rRNA methyltransferase. (rcsb.org)
If WD proteins form structures similar to G beta, their hydrodynamic properties should be those of compact, globular proteins, and they should be resistant to cleavage by trypsin. (embl.de)
Our working definition of folding was that the proteins from globular, trypsin-resistant structures because, except for G beta gamma, their functions are not known or cannot be assayed in reticulocyte lysates. (embl.de)

We chose proteins that include amino and carboxyl extensions as well as proteins that are made up entirely of WD-repeats. (embl.de)

One puzzle involves the ability of an IFIT protein previously known as IFIT1 to distinguish between cellular (mammalian) mRNAs and viral mRNAs. (elifesciences.org)
They are members of the rBAT family of mammalian proteins (TC #8.A.9). (tcdb.org)

The smaller proteins are generally of prokaryotic origin while the larger ones are of eukaryotic origin. (tcdb.org)
The larger eukaryotic and archaeal proteins possess N- and C-terminal hydrophilic extensions. (tcdb.org)

Not only does RNA translate the genetic code decoding it into protein, but it also has various catalytic and regulatory functions yet to be completely elucidated. (datexis.com)

We show that unlike G beta, several proteins with WD-repeats are able to fold into globular proteins in a rabbit reticulocyte lysate. (embl.de)

A majority of cellular proteins undergo post-translational modifications. (buffalo.edu)

PR domain-containing protein 9 (PRDM9) is a major regulator of the localization of meiotic recombination hotspots in the human and mouse genomes. (springer.com)
in an operon with an upstream PurR/LacI-type transcriptional regulator gene, named amlR ( ACSP50_2475 ), and a gene downstream ( ACSP50_2473 ) encoding a GGDEF-EAL-domain-containing protein putatively involved in c-di-GMP signaling. (frontiersin.org)

In the past, most of the attention was focused primarily on protein kinases and on their regulation, mainly because phosphatases were then viewed as simple housekeeping enzymes. (ac.be)
But advances in the understanding of protein phosphatases make now clear that these enzymes are precisely regulated and are as important as kinases in the regulation of cellular processes involving protein phosphorylation. (ac.be)

We demonstrate here that GSK-3 maintains the MLL leukemia stem cell transcriptional program by promoting the conditional association of CREB and its coactivators TORC and CBP with homedomain protein MEIS1, a critical component of the MLL-subordinate program, which in turn facilitates HOX-mediated transcription and transformation. (stanford.edu)
protein_coding" "Cz03g18100.t1","No alias","Chromochloris zofingiensis","Spindle assembly checkpoint component Mad1 [Interproscan]. (ntu.edu.sg)

These are frequently activated by fusion to other transcriptional proteins resulting in chimeric transcription factors. (stanford.edu)
The heterochromatin-enriched HP1 proteins play a critical role in regulation of transcription. (cipsm.de)
Previous studies have indicated that KIN17 is involved in global genome repair, DNA replication, transcription and regulation of the cell cycle as part of a multi-protein complex. (spandidos-publications.com)

Our studies have demonstrated that several of the proteins encoded by cellular oncogenes function in fundamental aspects of gene regulation. (stanford.edu)
Protein conformation is critically linked to function and often controlled by interactions with regulatory factors. (cipsm.de)
Hydrophilic microenvironment required for the channel-independent insertase function of YidC protein. (nurekilab.net)

RNA-binding proteins (RBPs) exert a broad range of biological functions. (nature.com)

A possible homolog of Dot1 was found in archaea which shows the ability to methylate archaeal histone-like protein in recent studies. (wikipedia.org)

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for HOS4 . (yeastrc.org)
Both homologous recombination, in which the ends of the broken DNA seek out intact templates with the same sequence, and nonhomologous end-joining pathways are found in Saccharomyces as they are in humans. (brandeis.edu)
These sequences represent the protein coding region of the dhp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. (genscript.com)
GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. (genscript.com)
Download DNA or protein sequence, view genomic context and coordinates. (yeastgenome.org)
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. (yeastgenome.org)
Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein. (yeastgenome.org)

In eukaryotes, G protein-coupled receptors (GPCRs) are the largest and most diverse group of membrane receptors and these receptors play an important role in mediating a variety of physiological responses such as responses to hormones, neurotransmitters, and environmental stimulants. (buffalo.edu)
The trimeric Sec61/SecY complex is a protein-conducting channel (PCC) for secretory and membrane proteins. (cipsm.de)

We are studying the effects and consequences of protein fusion on the transcriptional and transforming activities of these proteins using in vitro and animal models. (stanford.edu)
However, the only studied example of a WD-repeat protein, G beta, synthesized in vitro in a rabbit reticulocyte lysate, is unable to fold into a native structure without its partner protein G gamma. (embl.de)
It is not known whether all WD-repeat proteins are unable to fold when synthesized in an in vitro system. (embl.de)

Every modification adds to the functional diversity of the proteome by reversibly or irreversibly converting one protein species into another that potentially is a functionally distinct species. (cd177signaling.com)

In this regard, limited proteolysis is special as it has the unique ability to irreversibly convert one into two distinct protein species while at the same time generating new protein termini serving as attachment sites for even further PTM. (cd177signaling.com)

WD40 repeats usually assume a 7-8 bladed beta-propeller fold, but proteins have been found with 4 to 16 repeated units, which also form a circularised beta-propeller structure. (embl.de)
several WD40-containing proteins act as key regulators of plant-specific developmental events. (embl.de)
There are 1918936 WD40 domains in 345139 proteins in SMART's nrdb database. (embl.de)
The complete taxonomic breakdown of all proteins with WD40 domain is also avaliable . (embl.de)
Click on the protein counts, or double click on taxonomic names to display all proteins containing WD40 domain in the selected taxonomic class. (embl.de)

Here, we present the X-ray structure of the Nep1 homolog from the archaebacterium Methanocaldococcus jannaschii in its free form (2.2 A resolution) and bound to the S-adenosylmethionine analog S-adenosylhomocysteine (SAH, 2.15 A resolution) and the antibiotic and general methyltransferase inhibitor sinefungin (2.25 A resolution). (rcsb.org)

In Saccharomyces cerevisiae , meiotic DSBs preferentially occur at nucleosome-depleted regions in gene promoters (Ohta et al. (springer.com)
RNA-binding proteins (RBPs) mediate pivotal cellular functions such as RNA transport, degradation or translation and represent key effectors of post-transcriptional gene regulation. (nature.com)
CD177 antigen is a protein that in humans is encoded by the CD177 gene. (cd177signaling.com)

However, the extent to which they determine global protein production remains poorly understood. (genesilico.pl)

Anatomy19

Organisms13

Chemicals and Drugs113

Analytical, Diagnostic and Therapeutic Techniques and Equipment26

Phenomena and Processes92

Disciplines and Occupations1

Technology, Industry, Agriculture3

Information Science4

Health Care3

Yeast15

TRNA methyltransferase1

S288c1

Genes4

Methylation8

Evolutionarily conserved2

Arginine methyltransferases1

Organism3

MRNA4

Highly conserved4

Residues2

Enzyme7

Interaction7

Subunit5

Roles6

Ribosomal protein2

Lysine2

Amino acids2

Histone proteins1

PRMTs1

Specificity2

PTMs8

Substrates1

Ribosome1

Signal transduction2

Cells1

Orthologous2

Escherichia1

Distribution of proteins2

Synthesis5

Chromatin4

Mutation1

Structures3

Carboxyl1

Mammalian2

Eukaryotic2

Catalytic1

Several proteins1

Cellular proteins1

Regulator2

Enzymes2

Component2

Transcription3

Function3

Biological1

Methylate1

Sequence7

Membrane2

Vitro3

Modification1

Species1

WD405

Methanocaldococcus1

Gene3

Remains poorly understood1