Methods in molecular medicine protocols cells cultured. Medical search. Frequent questions

At this time I also began working with mouse and human embryonic stem cells resulting in the publication of some of the first papers describing the osteogenic differentiation of mouse ES and human cells in vitro and in vivo . (nottingham.ac.uk)
In this chapter, we describe protocols for culture, maintenance, and cardiac differentiation of hiPSCs. (mssm.edu)
regulating the differentiation, proliferation, maintenance and sculpturing organs and tissues, functioning of immune system and the elimination of defective harmful cells. (benthamscience.com)
When either the sphere-forming system or the adherent system is used, some of the cancer cells can be propagated but other cells are lost because of cell death/apoptosis or differentiation. (nature.com)
Transforming growth factor-β (TGF-β) signaling pathway is a multifunctional cytokine and plays important roles in regulating cell proliferation, cell cycle, differentiation, migration, and apoptosis in a broad spectrum of tissues [ 12 ]. (degruyter.com)
Here, we employed a human pancreatic differentiation platform complemented with an shRNA screen in human pluripotent stem cells (PSCs) to identify potential drivers of early endoderm and pancreatic development. (mdpi.com)
Retinoic acid receptor beta is a nuclear receptor protein that binds to retinoic acid (RA) to mediate cellular signalling in embryogenic morphogenesis, cell growth, and differentiation. (bvsalud.org)
This study aimed to understand the role of RARß in regulating cell growth and differentiation of lung cancer stem cells. (bvsalud.org)
CCN1 also induced Jag1 expression in hepatic stellate cells, whereupon they interacted with hepatic progenitor cells to promote their differentiation into cholangiocytes. (jci.org)
However, molecular profiling provides an opportunity to access different means of developing positive cells and conducting appropriate differentiation through the required methods. (essaytyping.com)
Differentiation of the iPSC at the molecular level provides an opportunity for conducting different expression of genes and patterns which are similar to the normal functioning of the cardiac development. (essaytyping.com)
It possesses a unique forkhead domain combined with a fragment of the target gene to initiate transcription [ 10 ] and regulates various biological processes such as tumor cell differentiation, cell proliferation, and migration [ 11 ]. (hindawi.com)

A study published in 2021 reported the use of MX-1 cells to generate a doxorubicin-resistant breast cancer cell line. (cytion.com)
Bauer disc diffusion method and interpreted following the CLSI 2021 guidelines. (who.int)

The introduction of cell culture techniques has helped a lot in understanding the physiological processes like cell signalling, neurobiology, cell proliferation, pathogenesis of diseases, apoptosis and even more. (benthamscience.com)
In addition, cell cycle distribution analysis revealed that G0/G1 phase arrest was induced following pristimerin treatment in CAL‑27 and SCC‑25 cells, which was strongly associated with upregulation of p21 and p27, coupled with downregulation of cyclin D1 and cyclin E. Meanwhile, pristimerin induced significant apoptosis of CAL‑27 and SCC‑25 cells, alongside decreased levels of caspase‑3 and specific cleavage of poly (ADP‑ribose) polymerase. (spandidos-publications.com)
Pristimerin has also been reported to induce apoptosis of various human cancer cells, including in multiple myeloma ( 10 ), breast ( 11 ), liver ( 12 ), pancreatic ( 13 ) and prostate cancer ( 14 ). (spandidos-publications.com)
Pristimerin exhibited potent anti-proliferative and apoptosis-inducing effects on the OSCC cell lines CAL-27 and SCC-25. (spandidos-publications.com)
Cell apoptosis was analyzed by flow cytometry. (degruyter.com)
Moreover, TGFBR2 was a direct target of miR-128-3p, and its overexpression reversed the inhibitory effects of miR-128-3p overexpression on inflammation and apoptosis in LPS-induced HK2 cells. (degruyter.com)
miR-128-3p could inhibit apoptosis and inflammation by targeting TGFBR2 in LPS-induced HK2 cells, which might provide therapeutic strategy for the treatment of sepsis. (degruyter.com)
Cell culturing, cell growth and apoptosis, cell mediated immune responses. (up.ac.za)
miRNA-149 was underexpressed in gastric cancer tissues and cells, while overexpression of miRNA-149 promoted cell apoptosis, retarded cell cycle, and inhibited proliferation and migration in AGS and MKN28 cells. (hindawi.com)

BDNF, together with glial cell line-derived neurotrophic factor (GDNF) and other supplements, is commonly used to differentiate human pluripotent stem cell (hPSC)-derived neural progenitor cells into neurons (Brafman). (stemcell.com)
Organoids are the 3D self-organising structures grown from stem cells and generated from adult stem and progenitor cells. (bvsalud.org)
During development, stem cells and resulting progenitor cells are responsible for generating all the tissues and cells of an organism. (wisc.edu)
Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)

Therefore, functionally analyzing the individual types of the heterogeneous cells and determining their role in tumor pathogenesis are critical. (nature.com)
In particular, hiPSC-based models provide a powerful tool to identify genetic risk factors, and to study cellular and molecular mechanisms that contribute to the pathogenesis of a disease. (frontiersin.org)

Cell" for elucidating the intricate mechanisms. (benthamscience.com)
The post-doctoral candidate will utilize in vitro and in vivo models, developed at CTI-San Francisco (CTI-SF), to mechanistically determine mechanisms associated with cellular reprograming of cell state. (sciencecareers.org)
however, the antitumor effects and underlying mechanisms of pristimerin in oral cancer cells have not yet been identified. (spandidos-publications.com)
The underlying mechanisms of these effects were primarily mediated by G 1 phase cell cycle arrest and inhibition of the mitogen-activated protein kinase (MAPK)/extracellular signal-regulate kinase 1/2 (Erk 1/2) and protein kinase B (Akt) signaling pathways. (spandidos-publications.com)
Therefore, it is critical to explain the pathogenic mechanisms at the molecular level, providing a potentially effective treatment for sepsis. (degruyter.com)
The transient transfection method commonly studies the underlying cellular mechanisms and gene expression patterns in this cell line [2, 3]. (cytion.com)
It can be used to understand the molecular mechanisms behind disease progression and to screen and develop drugs to combat disease. (cytion.com)
Doxorubicin resistance MX-1 cell line was developed to study molecular mechanisms, i.e., epithelial-mesenchymal transition (EMT) and ABC transporter ABCB1. (cytion.com)
The research in our laboratory focuses on understanding the molecular mechanisms that regulate neural stem cells and neurodevelopment with the goal of applying this knowledge in the treatment of neurological disorders and injuries. (wisc.edu)
My laboratory is investigating the mechanisms governing the behaviors and functions of neural stem cells in both healthy conditions and in neurological diseases. (wisc.edu)
Using neural stem cells as model systems, we are investigating the molecular mechanisms that regulate neuronal development during postnatal period and their implications in human neurodevelopmental disorders such as Rett Syndrome, Autism, and Fragile X syndrome. (wisc.edu)
Therefore, this work has developed a set of in vitro and in vivo experiments with state-of-the-art methods to better understand disease mechanisms and to evaluate new therapies for salivary glands. (lu.se)

Results demonstrated that pristimerin was toxic against the two cell lines, and exhibited inhibitory effects against proliferation. (spandidos-publications.com)
Studies have used MX-1 cells for shRNA (short hairpin RNA) and non-coding RNA transfection to explore their effect on breast cancer cells proliferation and growth. (cytion.com)
A) The biological activity of Human Recombinant BDNF was tested by its ability to promote the proliferation of a neuroblastoma cell line stably expressing TrkB (BR6). (stemcell.com)
Cell proliferation was measured using a colorimetric assay method. (stemcell.com)
Poisson-event-based analysis of cell proliferation. (swansea.ac.uk)

Exposure to air pollutants, cigarette smoke, bacteria, viruses, and many others may cause injury to the epithelial cells that line the conducting airways and alveoli. (bvsalud.org)
METHODS: Trachea and lung organoids were derived from mixed cell populations of mice primary airway epithelial cells, fibroblasts, and lung microvascular endothelial cells and directly digested from the whole cell population in the distal lung. (bvsalud.org)
We also demonstrated that in an animal model of Sjögren's syndrome, which exhibit dry mouth symptoms, that hDPSCs-EVs could inhibit the acquisition of the senescent phenotype in salivary gland epithelial cells (SGECs) and alleviate the loss of glandular function. (lu.se)
In conclusion, hDPSCs-EVs reduced senescence of salivary gland epithelial cells in both murine irradiation and Sjögren's syndrome models and may become a promising future for xerostomia patients. (lu.se)

Noninvasive detection and imaging of molecular markers in live cardiomyocytes derived from human embryonic stem cells Biophysical Journal. (nottingham.ac.uk)
BDNF acts as a survival factor for human embryonic stem cells when plated on either feeder cells or Corning® Matrigel® (Pyle et al. (stemcell.com)

For example, after 1 hr exposures to 0.5 μ/ml, surviving fractions determined by colony formation assay were approximately 0.3 for cells from spheroids treated intact and 0.001 for single exponential phase cells. (researchgate.net)
Based on the clonogenic assay, spheroid assay, mRNA levels of stem cell transcription factors, and cell cycle being arrested at the G0/G1 phase, the suppression of RARß resulted in significant inhibition of A549 parental cell growth. (bvsalud.org)
The significant problems caused by variability of RNA templates, assay designs and protocols, as well as inappropriate data normalization and inconsistent data analysis, are widely known but also widely disregarded. (gene-quantification.org)
This obvious lack of standardization at every step of the assay ( Figure 1 ) is exacerbated by significant differences in sample processing, use of controls, normalization methods and quality control management and has serious implications for the reliability, relevance and reproducibility of RT-qPCR. (gene-quantification.org)
Next, we tested different methods to generate PCSS using a vibratome and evaluated the slices in terms of viability (by WST-1), gene expression (by qRT-PCR), secreted α-amylase activity (by α-amylase assay kit) and histological/light sheet fluorescence microscopy (LSFM) three-dimensional imaging. (lu.se)

In laboratory settings, glioma stem cells (GSCs) can be isolated by adding EGF and FGF to suspension cultures in the absence of serum, which is the so-called sphere-forming method, or by using adherent culture systems in which specific materials are coated on culture dishes 4 , 6 . (nature.com)
Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture . (wikipedia.org)

Maryam Tabrizian is Canada Research Chair holder in Regenerative Medicine and Nanomedicine, cross-appointed between Faculty of Medicine-Biomedical Engineering Department and the Faculty of Dental Medicine and Oral Health Sciences. (mcgill.ca)
She has established expertise in the design of advanced biomaterials and biointerfaces for their application in nanomedicine, regenerative medicine and Lab-on-a-chip devices. (mcgill.ca)
Our target applications are mainly focused on regenerative medicine, nanomedicine and Lab-on-a-Chip platforms. (mcgill.ca)

As such, our laboratory masters a broad expertise in surface modification techniques, namely chemical, molecular assembly and biological methods to cope with the highly divergent requirements for surface properties in biomedical applications. (mcgill.ca)
Layer-by-Layer self-assembly as bioactive interface on artificial and biological nanoparticulate templates for imaging, molecular therapy and tissue engineering. (mcgill.ca)
Significant expertise with an array of molecular and cell biological techniques, including genome editing and insertion methods, QPCR/Nanostring, mRNA IVT, transfection protocols, primary cell tissue culture and assays (human PBMCs, etc), multi-color flow cytometry, bioinformatics analysis and/ or in vivo models for oncology and other therapeutic areas. (sciencecareers.org)
Her studies use a combination of electrophysiological, biochemical and molecular biological approaches as well as disease models to understand the pathologies of certain aberrant pathways in disease processes. (buffalo.edu)
This method makes it possible to detect specific mRNA transcripts in any biological sample. (gene-quantification.org)
the silencing of FOXC1 inhibited the biological function of AGS and MKN28 cells. (hindawi.com)
In this study, we investigated the inhibitory effects of miR-149 on the biological functions of gastric cancer cells by targeting FOXC1. (hindawi.com)
Embryo morphology al ows options, the discovery of cell-free DNA in the evaluation of its growth, viability, and biological fluids has led to major advances in implantation capacity. (who.int)

In the following chapter, we will discuss the advantages and disadvantages of different methods of transfection. (benthamscience.com)
These cells can be easily propagated and used in various research laboratories for transfection studies. (cytion.com)

In a previous study, it was reported that oxLDL treatment induced nucleotide‑binding domain and leucine‑rich repeat containing family, pyrin domain‑containing 3 inflammasome activation in endothelial cells (ECs) under HG conditions, in a manner that could be effectively reversed by rosmarinic acid. (spandidos-publications.com)
ECs were treated with oxLDL under HG conditions in the presence or absence of ROS scavengers mitoTEMPO and NAC, p38 inhibitor SB203580, FOXO1 inhibitor AS1842856 or transfected with the TXNIP siRNA, before protein expression levels of intercellular adhesion molecule 1 (ICAM‑1), vascular cell adhesion molecule‑1 (VCAM‑1), phosphorylated vascular endothelial‑cadherin (VE‑cadhedrin), VE‑cadherin and zonula occludens‑1 (ZO‑1) were measured by western blotting. (spandidos-publications.com)
Endothelial cells (ECs) form a semi-permeable monolayer that separates the bloodstream from underlying tissues and regulate the infiltration of blood cells and proteins through the vessel wall ( 1 , 2 ). (spandidos-publications.com)
These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)

Enriched derivatives may be analyzed by next-generation technologies such as whole-genome sequencing [WGS], RNAseq, or single-cell sequencing so that viable cells may be used to establish organoid cultures and tested for drug sensitivity. (genengnews.com)

Although this heterogeneity is generally discussed in terms of pathological structures, examining the dynamic heterogeneity at the cellular level is fundamental to understanding the origins of the cells, potential therapeutic targets and the source of tumor recurrences 3 . (nature.com)
Regardless of the nomenclatures used, the CSCs (as the cells are commonly termed) that are isolated directly from patients' tumors are considered to serve as valuable tools that can enhance our understanding of tumorigenesis, therapeutic resistances and the functional heterogeneity of cancers in vitro and in vivo 5 . (nature.com)
The advent of the human induced pluripotent stem cells (hiPSCs) technology offers unprecedented opportunities for disease modeling, personalized medicine, and the development of new therapeutic interventions. (frontiersin.org)
One of his seminal works was the development of human tumor xenografts in immunodeficient mice models, which help predict the therapeutic efficacy of lab protocols before they are used in patients. (buffalo.edu)
A research study has shown the therapeutic activity of Vinorelbine, a microtubule toxin, that triggered cell death and polyploidy in MX-1 cells [4]. (cytion.com)
CONCLUSION: We conclude that researchers will be able to examine cellular involvement during organ formation and molecular networks because organoids come in a variety of morphologies and stages of development, and this organoid protocol may be used for modelling lung diseases as a platform for therapeutic purposes and suitable for personalised medicine for respiratory diseases. (bvsalud.org)
Recent advances in the field suggest that stem cell-derived EVs possess a therapeutic potential which is comparable to the cells of their origin. (bvsalud.org)
Several labs have shown that CTC enumeration and molecular characterization may be valuable predictors of patient outcome and response to therapeutic intervention. (genengnews.com)

The real-time reverse transcription polymerase chain reaction (RT-qPCR) addresses the evident requirement for quantitative data analysis in molecular medicine, biotechnology, microbiology and diagnostics and has become the method of choice for the quantification of mRNA. (gene-quantification.org)
d WHO Collaborating Centre for Arbovirus Reference and Research (Dengue/Severe Dengue), Tropical Infectious Diseases Research and Education Centre, Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. (who.int)

Clinical and Translational Medicine (2022). (fda.gov)
Molecular Pharmaceutics (2022). (fda.gov)

Expert clinical investigators present their best and most reliable methods for the molecular diagnosis of common genetic disorders. (vietbooks.info)
They contain not only essential day-to-day benchtop wisdom and instruction, but also offer possibilities for introducing new molecular genetic diagnostic tests, as well as invaluable advice on controls, quality standards, and interpretation. (vietbooks.info)
While human induced pluripotent stem cells (hiPSCs) provide novel prospects for disease-modeling, the high phenotypic variability seen across different lines demands usage of large hiPSC cohorts to decipher the impact of individual genetic variants. (frontiersin.org)
However, to realize the full potential of hiPSCs for disease modeling and drug screening, several challenges still need to be overcome, as there is the high variability between hiPSC lines, the risk of accumulating genetic aberrations when culturing hiPSCs, and the lack of standardized procedures for hiPSC generation as such (reviewed by Volpato and Webber, 2020 ). (frontiersin.org)
The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
We use primary cell culture to look at cytokine signaling, calcium signaling, and metabolic changes due to genetic risk factors. (scienceboard.net)
The work is mainly focused on large-scale sequencing and molecular genetic analyzes of mainly research samples. (scilifelab.se)

The Department of Biomedical and Clinical Sciences (BKV) is one of the university's largest departments with activities above all else within the Faculty of Medicine. (scilifelab.se)

Yan's studies, which have been central for advancing molecular neuroscience, are significant to the fields of both biology and medicine. (buffalo.edu)
these oft-cited studies have appeared in journals including Proceedings of the National Academy of Sciences , Neuron , Molecular Psychiatry and The Journal of Neuroscience . (buffalo.edu)

The report was published in September in Nature Protocols . (spectrumnews.org)

Malaria culture is a method for growing malaria parasites outside the body, i.e., in an ex vivo environment. (wikipedia.org)
Initial hopes that the ex vivo culture would lead quickly to the discovery of a vaccine were premature. (wikipedia.org)
The original method for the successful ex vivo propagation of P. falciparum described culture of the parasite under static conditions (Trager-Jensen method). (wikipedia.org)
This led to the basis of cell culture studies, where cells grown in controlled artificial conditions simulate the conditions prevailing in natural ones, therefore, presumed to act as those in in vivo conditions. (benthamscience.com)
Our results suggest that single cell-derived subclones from a patient can produce phenotypically heterogeneous self-renewing progenies in both in vitro and in vivo settings. (nature.com)
I was excited for the opportunity to hone my skills in cell culture and in vivo models while also learning new skills such as computational multivariate modeling. (scienceboard.net)
In vivo and in organized cells, and proper symmetry are healthy individuals, macrophages can characteristics of higher-quality embryos, which phagocytize DNA that has been passively point to healthy development and higher rates of released into the blood from apoptotic or necrotic implantation. (who.int)
Firstly, we constructed an in vivo murine model of 25 Gy irradiation-induced salivary gland damage to evaluate the potential of human dental pulp stem cell (hDPSCs)-derived EVs. (lu.se)
For PCSS, we developed protocols to produce viable slices of controled thicknesses which retained the ability to secrete functional α-amylase for at least two days in ex vivo culture. (lu.se)

Spheroids of EMT-6 mammary tumor cells were markedly more resistant to different exposure doses of Adriamycin (ADR) than monolayer cells in exponential or plateau growth phases. (researchgate.net)
In oncology, circulating tumor cells (CTCs) provide a good example of a new sample type that poses a challenge for traditional material handling and biobanking methodologies. (genengnews.com)

qRT-PCR was used to detect the expression of miRNA-149 and FOXC1 in gastric cancer tissues and cells. (hindawi.com)
Human gastric cancer cell lines AGS and MKN28 were cultured and transfected with miR-149 overexpression plasmid and its control or FOXC1 siRNA and its control. (hindawi.com)
In light of the advancements in tumor biology and molecular technology, further research into the molecular mechanism involved in gastric cancer and identification of biomarkers with high specificity and good targeting [ 3 ] is of great significance for the diagnosis and treatment of this condition. (hindawi.com)
The human gastric epithelial permanent cell line (GES-1), human gastric cancer cell lines (AGS, BGC-823, MKN45, and SGC-7901), and HEK293 cells were provided by the Biomedical Experiment Center of Xi'an Jiaotong University. (hindawi.com)

The advent of human induced pluripotent stem cell (hiPSC) technology has produced patient-specific hiPSC derived cardiomyocytes (hiPSC-CMs) that can be used as a platform to study cardiac diseases and to explore new therapies. (mssm.edu)
Human pluripotent stem cells, with their ability to proliferate indefinitely and to differentiate into virtually all cell types of the human body, provide a novel resource to study human development and to implement relevant disease models. (mdpi.com)
Human pluripotent cells primarily contribute to cardiovascular research. (essaytyping.com)
Through human pluripotent technology, the researchers have been able to apply different techniques in the extraction of pluripotent cells. (essaytyping.com)
Patients with induced pluripotent cells (iPSC) derived cardiomyocytes provides an opportunity to access different manifestations of cardiovascular disease. (essaytyping.com)
Also, the practice of using transgenic animals in scientific laboratories has provided an opportunity to address significant challenges faced in the extraction of the pluripotent cells. (essaytyping.com)
Several factors determine the rate and the models required to be used for the development of the desired pluripotent cells. (essaytyping.com)

I work on various biochemical and biophysical approaches to investigate and control cell-cell, cell-scaffold interactions. (nottingham.ac.uk)
In this study soil samples were analysed from endemic regions of the country, and B. anthracis isolates were identified by classical bacteriological and biochemical methods. (who.int)

This article argues that an important reason for this is the inappropriate use of molecular techniques, particularly in the field of RNA biomarkers, coupled with a tendency to exaggerate the importance of research findings. (gene-quantification.org)

This second round of EQA reveals good proficiency in molecular and serological diagnostics of these diseases in the Asia Pacific region. (who.int)

Lentivirus (LV)-NRP-1 was transfected into rat primary cortical neuronal cultures before a 2-h oxygen-glucose deprivation and reoxygenation (OGD/R) injury to neurons. (biomedcentral.com)
It also has a role in neurogenesis by promoting survival and growth of dorsal root ganglion cells, and hippocampal and cortical neurons (Binder & Scharfman). (stemcell.com)
they contain both neurons (green) and support cells called glia (purple). (spectrumnews.org)
We mostly culture neurons, astrocytes, and microglia. (scienceboard.net)

My first postdoctoral position was held jointly between Imperial College and the Royal London Hospital School of Medicine and Dentistry (with Professor Francis Hughes) looking at the roles of the nitric oxide and prostaglandin pathways in bone biology, specifically the osteoblast. (nottingham.ac.uk)
They can act as signalling molecules that regulate a number of life and death pathways in cells, and they are generated by the immune system to fight microbes. (otago.ac.nz)
Prof Hampton's research is focused on how understanding how cells sense and respond to oxidants, and how these pathways might be modulated to prevent and treat human disease. (otago.ac.nz)
The MX-1 cell line can be transfected transiently to elucidate gene expression patterns and cell signaling pathways. (cytion.com)
Since the process would also need glucose deprivation methods, then one of such methods should be applied with an aim of reducing the glucose levels in one of the required pathways. (essaytyping.com)

Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows . (wikipedia.org)
Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
[6] In 1885 Wilhelm Roux removed a section of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture. (wikipedia.org)
Gottlieb Haberlandt first pointed out the possibilities of the culture of isolated tissues, plant tissue culture . (wikipedia.org)
[12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)

Recent Ph.D. in molecular and cell biology, rare disease, cancer biology, immuno-oncology, immunology, or a related field, with a strong track record of scientific publications. (sciencecareers.org)
Conversion of Peripheral Blood NK Cells to a Decidual NK-like Phenotype by a Cocktail of Defined Factors The Journal of Immunology, 190:3939-3948 (2013). (bioind.com)

I was also affiliated with the Wolfson Centre for Stem Cells, Tissue Engineering and Modelling (STEM), part of the Centre for Biomolecular Sciences, which is now the Biodiscovery Institute. (nottingham.ac.uk)
My research continues to focus on stem cells and osteoblasts and in particular 3D cell culture models and microenvironments. (nottingham.ac.uk)
My research interests focus mainly on stem cells (embryonic, 'adult' and fetal origins) and their applications in tissue engineering, particularly the osteoblast and bone tissue. (nottingham.ac.uk)
Because most cancers have a clonal origin, cancer stem cells (CSCs) must generate phenotypically diverse progenies including mature CSCs that can self-renew indefinitely and differentiated cancer cells that possess limited proliferative potential. (nature.com)
However, the function of RARß in cancer stem cells (CSCs) has yet to be determined. (bvsalud.org)
Scientists typically make brain organoids by transforming cells from a person's skin, blood or urine into stem cells capable of turning into almost any cell type. (spectrumnews.org)
They then use chemicals to transform the stem cells into brain-cell precursors, and let them mature. (spectrumnews.org)
The researchers use a multi-step process: They coax stem cells to become brain-cell precursors in a standard way. (spectrumnews.org)
The researchers discovered that they could boost the proportion of stem cells that mature into brain cells if they started transforming the cells earlier - after letting stem cell colonies divide just once instead of 20 times over four to five months. (spectrumnews.org)
Stem Cells Dev. (spectrumnews.org)
Stem cells have two fundamental properties: self-renewal and multipotency. (wisc.edu)
In the adult, stem cells exist in many tissues throughout life and may play critical roles in physiological functions and tissue regeneration. (wisc.edu)
Neural stem cells in the postnatal brain have significant roles in both normal brain functions, such as learning and memory and the brain's response to injuries. (wisc.edu)
Stem Cells Int, 2017, 5718608. (lu.se)

As illustrated in this book, the marriage between basic science and clinical medicine is essential in our quest for these unknowns. (vietbooks.info)
the next decade must underscore translational research, bridging clinical medicine and basic science with re- ization of fundamental knowledge to improve prevention, diagnosis, and tre- ment of viral hepatitis C. (vietbooks.info)
Bankert, an internationally recognized expert working at the translational intersection between clinical medicine and basic science, has made major contributions to biomedical research. (buffalo.edu)
The present invention provides a simple and robust human liver cell-based system in which persistent hepatitis C infection, persistent hepatitis B infection or ethanol exposure induces a clinical Prognostic Liver Signature (PLS) high-risk gene signature. (sumobrain.com)
His research involves the application of economic methods to health technology assessment in a wide range of clinical settings, including cancer, mental health, and tuberculosis. (warwick.ac.uk)
You will work as a molecular biologist within the Clinical Genomics/Core facility with development work and service within genomics. (scilifelab.se)
Three hundred clinical samples were collected from male and female in and out-patients of all ages and processed using standard bacteriological methods. (who.int)

This activity varied according to concentration (100/500 μg/ml), multiplicity of infection (0.1/0.01), and cell type (Vero E6/Caco-2 cells). (frontiersin.org)
Detection of acute/recent DENV infection by both molecular (RT-PCR) and serological methods (IgM) was available in 19/24 (79.2%) participating laboratories. (who.int)

For example, the mesoderm formation (BRY and MIXL) is one of the initial steps that is used in separating and differentiating various cardiac cells. (essaytyping.com)

For this purpose, we performed in vitro assays, proving that Lf exerts an antiviral activity against SARS-COV-2 through direct attachment to both SARS-CoV-2 and cell surface components. (frontiersin.org)
A novel, integrated in vitro carcinogenicity test to identify genotoxic and non-genotoxic carcinogens using human lymphoblastoid cells. (swansea.ac.uk)

Humana Press, p. 131-146 (Methods in molecular biology (Clifton, N.J. (lu.se)

The extraction process has been facilitated through the recent development in genomics and molecular medicine which has revolutionized research thus providing an opportunity to access important methods reliable for adopting scientific research. (essaytyping.com)

Cell lines adapted to survive in laboratory conditions are of little value when it comes to predicting how their wild-type relatives will react when exposed to a drug candidate. (genengnews.com)
The work also includes collaboration with various research groups, other CG units and the university hospital's Precision Medicine Laboratory for the development of new technologies, instrument acquisition and use. (scilifelab.se)

In the present study, the anticancer potential of pristimerin was investigated in two oral squamous cell carcinoma (OSCC) cell lines, CAL‑27 and SCC‑25. (spandidos-publications.com)
Cell-based anticancer drug screening generally utilizes rapidly proliferating tumour cells grown as monolayer cultures. (researchgate.net)
The aim of this study was to examine the potential usefulness of 3-D cultured tumour cells for anticancer drug screening. (researchgate.net)

Mitochondria, a powerhouse of the cell, are rich in a large number of enzymes, which not only participate in cellular oxidative phosphorylation and ATP synthesis, but also regulate intracellular calcium ions and reactive oxygen species to maintain cellular homeostasis. (biomedcentral.com)

He was the leading proponent of the idea that cancer cells cannot grow in culture because they are deprived of the cancer patient's tumor-microenvironment. (buffalo.edu)
MX-1 cells can be used for screening potential drugs against breast cancer as they mimic the cancer cell microenvironment. (cytion.com)
Thus, other factors related to the metabolic state of the cells, the microenvironment, or the formation of different drug products must account for the observed resistance. (researchgate.net)
Tissues may be microdissected into cell subpopulations, such as tumor and microenvironment, and plasma samples may be enriched for circulating DNA, such as tumor DNA in cancer patients,"Dr. Chadwick adds. (genengnews.com)

The process is 10 times faster than the usual method, says lead investigator Alysson Muotri , professor of pediatrics and of cellular and molecular medicine at the University of California, San Diego. (spectrumnews.org)

The brain-like blobs can be kept alive for more than two years, maturing long enough to develop the cell types, activity patterns and gene-expression profiles of a typical human brain after birth. (spectrumnews.org)
The processes are marked by gene expression which can only be profiled through molecular methods. (essaytyping.com)

Thus, a much higher grade of parallelization, and throughput in the production of hiPSCs is needed, which can only be achieved by implementing automated solutions for cell reprogramming, and hiPSC expansion. (frontiersin.org)
We have developed a feeder-free, Sendai virus-mediated reprogramming protocol suitable for cell culture processing via a robotic liquid handling unit that delivers footprint-free hiPSCs within 3 weeks with state-of-the-art efficiencies. (frontiersin.org)
Automatically expanded hiPSCs exhibit normal growth characteristics, and show sustained expression of the pluripotency associated stem cell marker TRA-1-60 over at least 5 weeks (10 passages). (frontiersin.org)

This is carried out through design, fabrication and characterization of multifunctional and bioactive surfaces as well as developing methodologies and protocols for creating these new biointerfaces. (mcgill.ca)
The development of the cells has been challenging especially through the current methodologies. (essaytyping.com)

Its anti-inflammatory activity is mediated by the ability to enter inside the nucleus of host cells, thus inhibiting the synthesis of proinflammatory cytokine genes. (frontiersin.org)
Bankert also has four patents for his discoveries of coated cells, microflotation devices and liposomal microparticulate cytokine preparation methods. (buffalo.edu)

The disease models, therefore, offer an insightful opportunity to obtain and understand the molecular basis if the cardiovascular diseases. (essaytyping.com)

I are a 15 Cancer Cell Culture: Methods and Protocols (Methods in Molecular Medicine) complex first high property. (opa-city.com)
The cancer stem cell (CSC) hypothesis, which was first described in studies on leukemia, has attracted considerable attention in other cancer fields, including the one devoted to brain tumor 4 . (nature.com)
His research defines the molecular and cellular events that shape the function of human tumor associated lymphocytes - a type of white blood cell - to design and develop cancer therapies. (buffalo.edu)
MX-1 is a human breast adenocarcinoma cell line derived from a breast cancer xenograft model in athymic mice. (cytion.com)
MX1 cells are triple-negative breast cancer (TNBC) that are extensively utilized for research on drug resistance, cell signaling, drug response mechanism, etc. (cytion.com)
MX-1 cells are considered a type of triple-negative breast cancer (TNBC) [1]. (cytion.com)
This human breast adenocarcinoma MX-1 cell line is commonly used in breast cancer studies instead of cells such as MDA-MB-231 or MCF-7. (cytion.com)
Here are a few special applications of this cell line in cancer research. (cytion.com)
MX-1 cells are used to study the molecular factors mediating the development of resistance against breast cancer drugs. (cytion.com)

When we placed dissociated glioma tissues on uncoated culture dishes, some cells formed sphere-like aggregates, whereas other cells grew out and extended cellular processes ( Fig. 1a ). (nature.com)
hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)

Herein, we assessed the potential of multimodal imaging traceable myelin-targeting liposomes to quantify myelin in organotypic cultures. (researchgate.net)

Screening of monolayer and spheroid cultures was found to identify different sets of hit compounds. (researchgate.net)
This resistance was not due to differences in the cell cycle state of these inner cells since separate experiments showed that both exponential and plateau phase monolayer cells were about equally sensitive when the surviving fraction was plotted vs absorbed drug (D0, = 0.04 μ/106 cells). (researchgate.net)

90% are squamous cell carcinomas. (spandidos-publications.com)
This cell line is a sound model system for breast carcinomas. (cytion.com)

In this chapter, we will provide general overview of various technical approaches for detecting apoptotic cells. (benthamscience.com)
Therefore, stem cell-based approaches to tackle the condition are currently a focus of regenerative therapies for COPD. (bvsalud.org)

Infected human red blood cells are incubated in a culture dish or flask at 37 °C together with a nutrient medium and plasma, serum or serum substitutes. (wikipedia.org)
injected 6 wild-caught Chacma baboons ( Papio ursinus orientalis ) with pooled HBV-positive serum and analyzed the baboons for 52 weeks by using sensitive molecular techniques to detect evidence of transmission ( 16 ). (cdc.gov)
BIO-MPM-1 Medium is a multi-purpose, serum-free medium for culture of a wide variety of anchorage-dependent cells. (bioind.com)
The level of miR-128-3p was decreased, and TGFBR2 expression was increased in serum samples of sepsis patients and LPS-induced HK2 cells. (degruyter.com)
Module A contained serum samples spiked with cultured dengue virus (DENV) or chikungunya virus (CHIKV) for the detection of nucleic acid and DENV non-structural protein 1 (NS1) antigen. (who.int)

The cationic features of Lf bind to cells, protecting the host from bacterial and viral injuries. (frontiersin.org)
Interestingly, the in silico results strongly supported the hypothesis of a direct recognition between Lf and the spike S glycoprotein, which can thus hinder viral entry into the cells. (frontiersin.org)
Viral culture is also related, with cells as hosts for the viruses. (wikipedia.org)

Sputum culture grew Mycobacterium avium complex. (medscape.com)

Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)

Recently, the molecular mechanism of miRNAs in tumors has been a popular research topic in tumor-related fields. (hindawi.com)

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