Methods fluorescent antibody technique. Medical search. Frequent questions

The rising prevalence of rabies in both animals and humans has had a direct impact on the growth of the fluorescent antibody test (FAT) market. (databridgemarketresearch.com)
Rabbit antibodies can recognize a number of targetable epitopes on humans than rodent antibodies which is pivotal in basic research and in pre-clinical trials. (news-medical.net)
At present, two amplification methods have been FDA approved for detection of M. tuberculosis from sputum in humans: the Amplicor system (Roche Molecular Systems, Branchburg, NJ) and the AMTD system (Gen-Probe Inc., San Diego, CA). The Amplicor system has as its target the mycobacterial 16S ribosomal RNA gene. (vin.com)

The fluorescent antibody test (FAT) is a method for diagnosing rabies in fresh or frozen brain tissues that is based on antigen detection and is recommended by the World Health Organization (WHO) and the World Organization for Animal Health (OIE). (databridgemarketresearch.com)
The fluorescent antibody test (FAT) market is segmented on the basis of technology, product, method, indication, antigen and end-user. (databridgemarketresearch.com)
Developed in 1971 by Engvall and Perlmann, the method quantifies peptides, proteins, antibodies, and hormones present in a sample by immobilizing an antigen on a solid surface before it is complexed with an antibody that is associated with an enzyme. (news-medical.net)
A monoclonal antibody called rituximab can bind to the B lymphocyte's Clusters of Differentiation (CD) 20 antigen and eliminate B lymphocytes from the bloodstream. (ijpsonline.com)
For a long time, some scholars have tried to combine antibody molecules with some tracer substances, and use antigen-antibody reaction to locate antigenic substances in tissues or cells. (medicilon.com)
This technique of labeling antibodies with fluorescent substances for antigen localization is called fluorescent antibody technique ( fluorescent antibody technique ). (medicilon.com)
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). (gentaur.com)
This method develops rabbit antibody libraries in single-chain variable format (scFv) as well as in antigen binding fragment (Fab) format. (news-medical.net)
Indirect methods include detection of antigen or antibody, and measures of cellular reactivity against mycobacterial antigen. (vin.com)
The sensitivity of antigen detection is increased significantly by tyramide signal amplification (TSA) compared to the conventional peroxidase labelled Avidin-Biotin complex (ABC) technique. (ox.ac.uk)
HIV-1 p24 antigen was also detected in PM brain tissue by TSA enhanced immunofluorescence and demonstrated increased sensitivity compared to the conventional immunofluorescence technique with a greatly reduced autofluorescence background. (ox.ac.uk)
In dot blots, we could successfully detect rabbit IgG against firefly luciferases, Limpet Haemocyanin, and SARS-CoV-2 Nucleoprotein (1-250 ng), as well as the antigen bound antibodies using either CCD imaging , and even photography using smartphones . (chagghana.org)
The urine albumin-antigen complexes with the solid-phase antibody. (cdc.gov)

Blood samples were collected for CT IgG antibodies testing using indirect immunofluorescence. (ufrgs.br)
Material/Methods: In this paper we compare cellular and humoral immunitythrough in vitro proliferation studies, ELISA and immunofluorescence tests in actinic prurigo patientsand healthy controls. (medscimonit.com)
Parasite-infected-cell-agglutination and indirect immunofluorescence assays for detection of human serum antibodies bound to antigens on Plasmodium falciparum-infected erythrocytes. (ox.ac.uk)
An antibody-mediated parasite-infected-cell-agglutination assay (without secondary antibody) and an indirect immunofluorescence assay employing an anti-Fc secondary reagent were used to detect bound antibody. (ox.ac.uk)
Serum from 'non-immune' Europeans did not agglutinate infected erythrocytes, however, in the immunofluorescence test with anti-Ig and anti-F(ab')2 secondary reagents we could detect the binding of IgG antibody from 'non-immune' European serum to a small proportion of infected cells. (ox.ac.uk)
The recombinant nucleoprotein-based Crimean-Congo hemorrhagic fever virus antibody detection systems for sheep sera were developed by enzyme-linked immunosorbent assay (ELISA) and an indirect immunofluorescence assay techniques. (utmb.edu)
The ELISA and indirect immunofluorescence assay using recombinant nucleoprotein of the virus proved to have high sensitivity and specificity for detecting the immunoglobulin G antibodies to the virus in sheep sera. (utmb.edu)
[ 11 ] Further studies showed that tubular smooth-muscle antibody (SMA-T) and glomerular smooth-muscle antibody (SMA-G) immunofluorescence staining patterns, which predominantly react with filamentous actin (F-actin), were the main antigenic moiety of smooth-muscle antibodies. (medscape.com)
The earliest experiments for detection of smooth-muscle antibodies involved indirect immunofluorescence (IIF). (medscape.com)
Immunofluorescence techniques in diagnostic microbiology / edited by Joan M. B. Edwards, C. E. D. Taylor, A. H. Tomlinson. (who.int)

This conjugate pad along with a nitrocellulose pad layered with a monoclonal antibody (McAb, 4B7F9) against EBOV-VP40 and goat anti-rabbit Ig, sample application pad, and absorbent pad were assembled together to form a lateral flow test strip. (news-medical.net)

Assays using monoclonal antibodies enable the measurement of individual antigens but interpretation can be confounded by cross-reactivity between fungal species. (cdc.gov)

HCV assay by direct plaque procedure is rapid and convenient and gives infectivity titres identical to the fluorescent focus assay technique. (microbiologyresearch.org)
These days, immunohistochemical staining is just one of several established immunostaining techniques, including enzyme-linked immunosorbent assay, flow cytometry, immuno-electron microscopy, and Western blotting. (news-medical.net)
The assay allows for the simultaneous detection of more than one type of molecule because particles of different sizes can be used to tag different antibodies. (news-medical.net)
The Blood TB Test (BTB) is currently based on two assays, a lymphocyte transformation assay and an ELISA to measure antibody formation against antigens (see below). (vin.com)
The FIA assay resembles the radio-immunoassay (RIA) in technique and sensitivity without the potential health hazards associated with the handling of isotopes in the laboratory. (cdc.gov)
The enzyme-linked immunosorbent assay (ELISA) technique could easily be performed in resources constraint setting. (researchsquare.com)

However, they are all methods that rely on the use of antibodies to detect and identify proteins within biological samples. (news-medical.net)
The details of how proteins are moved around within a cell are being deciphered with high-powered microscopy combined with sophisticated techniques for labeling individual molecules, including fluorescent or "quantum dot" tags and antibodies tailored to adhere to a protein in a particular state of activation. (alzforum.org)
These painstaking methods are now being applied to investigating how molecules are moved around in neurons and how the proteins that characterize neurodegenerative diseases may disrupt one or more of these mechanisms. (alzforum.org)
Using carbon nanotubes, MIT chemical engineers have devised a new method for detecting proteins, including fibrinogen, one of the coagulation factors critical to the blood-clotting cascade. (mit.edu)
The new method is the first to create synthetic recognition sites (similar to natural antibodies) for proteins and to couple them directly to a powerful nanosensor such as a carbon nanotube. (mit.edu)
UC Berkeley engineers attached SARS-CoV-2 "spike" proteins to the surface of liposomes, creating lab-made mimics of the deadly virus called "spike-liposomes," which, when paired with a new DNA-patterning technique, could enable efficient testing of antibody therapies. (sflorg.com)
This microscope image of their technique shows that mixing spike-liposomes (top right, tagged with green fluorescent protein) with ACE2 receptor (bottom red, tagged with red fluorescent protein), results in a composite of both proteins (left), indicating that their spike-liposomes bind to ACE2 receptor in the same way as SARS-CoV-2 virus. (sflorg.com)
It was noted by the authors that this method was free from interferences from proteins and bacterial cells so it might have applicability to biological fluids such as blood or urine. (cdc.gov)

5 days after onset of symptoms, serologic techniques are used to detect IgM and/or IgG responses to the virus. (cdc.gov)
The initial principle outlined that antibodies labeled with a fluorescent dye could detect pneumococcal antigens in infected tissues. (news-medical.net)
There are a number of methods available to laboratories to detect RSV in respiratory specimens from patients. (quidel.com)
Traditionally, laboratories used viral culture to detect influenza virus and improvements in virus culture techniques allowed for results within 48-72 hours. (quidel.com)
Molecular methods such as reverse transcription polymerase chain reaction (RT-PCR) based tests are the most accurate methods to detect RSV, but are expensive and can only be done in laboratories that can afford expensive equipment and employ highly trained technologists. (quidel.com)
When incubated with a blot on which HRP-conjugated antibodies (or other probes) are bound, a chemical reaction emits light at 425 nm which can be captured with x-ray film, CCD camera imaging devices and phosphorimagers that detect chemiluminescence. (thermofisher.com)
Methods that directly detect bacteria from clinical specimens include acid fast and fluorescent smears, culture, and nucleic acid amplification such as polymerase chain reaction (PCR). (vin.com)
Recent technological advances have seen the introduction of methods that detect mycobacteria by amplification of mycobacterial nucleic acids (DNA or RNA) followed by detection of the amplification product. (vin.com)
Ideally, these methods can detect as few as 1-10 organisms. (vin.com)

This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. (gentaur.com)
This complex then reacts with fluorescein-labeled antibody. (cdc.gov)
Here the conjugation of affinity markers (e.g. antibodies, DNA etc.) with fluorescent compounds like fluorescein isothiocyanate (FITC), phycoerythrin (PE) cyanin- or Alexa-dyes is most commonly used. (jakenzyme.com)
These primary antibodies are then visualized by tagging them with a fluorescein conjugated anti-immunoglobulin antibody, which serves as the secondary antibody. (medscape.com)

Thus, we focused our analysis on 2 other serologic CHIKV detection methods: ELISA and immunochromatography test for rapid detection (RDT). (cdc.gov)
Antibodies to the glycoprotein of Rabies virus present in those sera have been titrated using the Platelia ELISA Kit, an indirect ELISA developed and manufactured by BIO-RAD (Marne-la Coquette- France). (researchsquare.com)

They include assays for HIV SEROPOSITIVITY and HIV SERONEGATIVITY that have been developed for screening persons carrying the viral antibody from patients with overt symptoms of AIDS or AIDS-RELATED COMPLEX. (bvsalud.org)
These assays are suitable for studies on the antigenic diversity of erythrocyte antigens in natural infections and specific antibody responses to these antigens in infected patients. (ox.ac.uk)

These techniques were based on the Halogen Immunoassay (HIA), an immunostaining technique that detects antigens associated with individual airborne particles >1 microm, with human serum immunoglobulin E (IgE). (cdc.gov)
Fluorescence immunoassay technology is one of the earliest developed labeling immunoassay techniques. (medicilon.com)
A solid-phase fluorescent immunoassay for the measurement of human urinary albumin is described by Chavers et al. (cdc.gov)
The fluorescent immunoassay is a noncompetitive, double-antibody method for the determination of human albumin in urine. (cdc.gov)
Results of the fluorescent immunoassay (FIA) are reproducible, and the test is accurate and sensitive for the detection of human urinary albumin excretion. (cdc.gov)
The fluorescent immunoassay is a non-competitive, double-antibody method for the determination of human albumin in urine. (cdc.gov)

When the physical and chemical characteristics of cells or particles are sought to be determined, flow cytometry is often the most suitable method. (news-medical.net)
For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. (gentaur.com)

Antibodies titers covered a wide range of values. (researchsquare.com)
Baseline antibody titers did not differ significantly among groups but B/Brisbane titers were too high for meaningful response assessments. (cret-signal.com)
Negative titers for anti-smooth-muscle antibody (ASMA) are expected in healthy individuals. (medscape.com)

Confocal image of C57BL/6 mouse lung sample acquired using the IBEX method of highly multiplexed antibody-based imaging: CD11c (magenta) in Cycle 1, CD206 (blue) in Cycle 1, and CD31 (green) in Cycle 4. (biolegend.com)
Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. (biolegend.com)

Fluorescence immunotechnology generally uses fluorescent substances for labeling. (medicilon.com)
Fluorescent molecules do not convert all the absorbed light energy into fluorescence, and they are always released in more or less other forms. (medicilon.com)
Fluorescence efficiency refers to the percentage of fluorescent molecules that convert the absorbed light energy into fluorescence, which is proportional to the value of the emitted fluorescent light quantum. (medicilon.com)
Each fluorescent molecule has its specific absorption spectrum and emission spectrum (fluorescence spectrum), that is, there is a maximum absorption peak and maximum emission peak at a specific wavelength. (medicilon.com)
When the wavelength of the excitation light is selected to be close to the maximum absorption peak wavelength of the fluorescent molecule, and the measured light wave is close to the peak of the maximum emission light, the obtained fluorescence intensity is also maximum. (medicilon.com)
FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. (gentaur.com)
The fluorescence of the stable solid-phase antibody complex with a fluorometer. (cdc.gov)
Consequently, fluorescence detection remains the dominant optical detection technique in microfluidics. (jakenzyme.com)

The method involves selectively identifying antigens in a sample of cells within a tissue section through the principle that certain antibodies will bind to specific antigens present in the tissue. (news-medical.net)
Immunoassays are widely used for detection of antibodies against specific antigens in diagnosis, as well as in electrophoretic techniques such as Western Blotting. (chagghana.org)

Two methods are described for detecting the binding of serum antibodies from adults in an endemic malarious area (The Gambia) to surface antigens on Plasmodium falciparum-infected erythrocytes. (ox.ac.uk)
We have found that more than 95.80% (114/119) of cats had a serum with antibodies titer above 0.5 EU /ml so like 74.75% (n = 305) of dogs. (researchsquare.com)
In this study, we aim to initiate a preliminary investigation of vaccination efficacy in Ivory Coast using results obtained when were quantified anti rabies antibody titer in dog and cat serum samples. (researchsquare.com)
This technique involves subjecting thin specimens of rodent liver, stomach, or kidney to a patient's serum. (medscape.com)
If present in the patient's serum, antibodies attach to smooth-muscle antigens on the rodent tissue specimens. (medscape.com)

Both amplification products are detected through fluorescent probes against the generated nucleic acids. (vin.com)
The numerous chemiluminescence (CL) applications in microfluidic analysis systems using immobilized enzymes, antibodies or nucleic acids have been recently described [37-39]. (jakenzyme.com)

Direct evidence of the associations between fungal fragments and building-related disease is lacking and in order to gain a better understanding, it will be necessary to develop diagnostic reagents and detection methods, particularly for submicron particles. (cdc.gov)
Fluorescent reagents are growing in popularity for western blotting because they offer increased time savings over chemiluminescent detection and reduced chemical waste compared to both chemiluminescent or chromogenic detection systems. (thermofisher.com)

Although IFA tests have good sensitivity and specificity ( 10 ) for CHIKV, this method requires specific material that may not be available in diagnostic laboratories worldwide. (cdc.gov)
Microscopy with direct fluorescent antibody testing (DFA) is considered the test of choice for diagnosis of giardiasis since it provides increased sensitivity over non-fluorescent microscopy techniques. (cdc.gov)
Most importantly, chemiluminescence yields the greatest sensitivity of any available detection method. (thermofisher.com)

This is achieved by isolating antibody variable domain repertoires from the B-cell complementary DNA (cDNA) obtained from the rabbit spleen and amplifying it into a Fab repertoire by the polymerase chain reaction (PCR) method. (news-medical.net)

High-throughput automated fluorescent microscope imaging technologies enable the experimental determination of a protein's sub-cellular localization and its dynamic trafficking within a range of cellular contexts. (biomedcentral.com)

Comparatively, the new fusion protein displays slightly higher and more sustained luminescent signal when compared to commercial HRP-labeled secondary antibodies, constituting a novel promising alternative for Western Blotting and immunoassays. (chagghana.org)

The rising occurrence of rabies as a result of dog bites, favourable government policies and laws, and rising funds for research and development are some of the factors that will propel the fluorescent antibody test (FAT) market during the forecast period. (databridgemarketresearch.com)
On the other hand, increasing costs associated with rabies diagnosis, as well as a lack of consumer awareness about advanced technologies, will act as a market restraint for the fluorescent antibody test (FAT) market during the forecast period. (databridgemarketresearch.com)
We aim to investigate anti Rabies vaccination efficacy in Ivory Coast, using data obtained purposely for antibody quantification prior to international movement of pets. (researchsquare.com)

Whole-brain volumetric microscopy techniques such as serial two-photon tomography (STPT) can provide detailed information on the roles of neuroinflammation and neuroplasticity throughout the whole brain post-stroke. (frontiersin.org)

The immunochemical specificity of the immunofluorescent Crithidia luciliae method for detection of antibodies to double-stranded DNA (dsDNA) was confirmed by demonstrating abolition of staining by DNase digestion and by absorption with dsDNA. (nih.gov)

These procedures allow the detection of cellular fluorescent signals throughout the brain in an unbiased manner. (frontiersin.org)

The surface of erythrocytes containing mature parasites bound antibody, but the surface of uninfected cells or cells containing early parasite stages did not react. (ox.ac.uk)

In contrast to the results with freshly collected isolates, antibodies from sera of Gambian adults did not bind to the surface of infected cells from five different culture-adapted isolates of P. falciparum. (ox.ac.uk)

Immuno-electron microscopy, also referred to as EM immunolabelling and immuno-EM, is a technique that tags antibody molecules with electron-dense substances, usually, and most effectively, being small gold particles, which are seen during the analysis as easy to spot dark dots. (news-medical.net)
The radiation ability of fluorescent molecules will be weakened or even quenched after being irradiated by the excitation light for a long time. (medicilon.com)

In recent years, several special fluorescent immunoassays have been developed, which are used in clinical tests like enzyme immunoassays and radioimmunoassays. (medicilon.com)

Antibody to human albumin is covalently attached to derivatized polyacrylamide beads. (cdc.gov)
Alternative detection methods for pathogen sensing include the application of silver dots for direct optical density measurements using a scanometric reader [45,46], or biosensors using resonance light scattering (RLS) techniques based on nanometer-sized metallic particles (mostly gold) covalently linked to antibodies. (jakenzyme.com)

Chemiluminescent substrates are popular because they offer several advantages over other detection methods. (thermofisher.com)
While the detection limits are still not as low as chemiluminescent detection, fluorescent detection has the unique advantage of allowing multiple targets to be assayed for on the same blot at the same time without the need to strip and reprobe. (thermofisher.com)
They usually rely on colorimetric, fluorescent or chemiluminescent methods for detection. (chagghana.org)

While high throughput imaging and automated analysis techniques are extremely useful, they suffer by removing the trained researcher from actually examining the majority of the images. (biomedcentral.com)
Other methods that are used to produce rabbit mAbs and to analyze immune antibody repertoires are high-throughput DNA technologies with mass spectrometry. (news-medical.net)

A new in vitro method (END) for detection and measurement of hog cholera virus and its antibody by means of effect of HC virus on Newcastle disease virus in swine tissue cultures. (microbiologyresearch.org)
It is especially useful for measurement of low levels of urinary albumin not detectable by dipstick methods. (cdc.gov)
In recent years, new serological techniques have suppemented the measurement of spleen rate and parasite rate. (who.int)

Some non-fluorescent pigment substances such as methylene blue and basic fuchsin are commonly used in fluorescent antibody technology. (medicilon.com)

The indirect fluorescent antibody test (IFA) was the serological test employed in this laboratory. (who.int)

Two weeks post-stroke, mice received intramuscular injections of pseudorabies virus (PRV-152), a trans-synaptic retrograde herpes virus driving expression of green fluorescent protein (GFP), into the affected contralesional forelimb to label neurons in descending tracts to the forelimb musculature. (frontiersin.org)

Dr. Perez Garcia concluded that if the hemato encephalic [blood-brain] barrier could be crossed, then a therapeutic technique using insulin could be applied to all kinds of illnesses in which the major problem was getting the remedial drug into the cell interior. (iptq.com)

If Giardia is not found after 3 stool exams, and if a parasite concentration method is used to process the stool specimen before the exam, it is highly probable that the patient is no longer infected. (cdc.gov)
The TSA method also permitted the use of a lower concentration of primary antibody than is conventionally used. (ox.ac.uk)

To illustrate our imaging techniques and automated image quantification, we examined long-term post-stroke motor circuit connectivity in mice that received a motor cortex photothrombotic stroke. (frontiersin.org)

Having reviewed the various materials and detection methods employed in lab-on-a-chip devices, we now provide a detail list of LOC studies grouped by class of target analytes.3. (jakenzyme.com)

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. (biolegend.com)

The method of the fluorescent marked antibodies proves the existence of herpes virus in the tissue culture and also in the corneal epithelia of clinical infections. (dtic.mil)

The technique has become invaluable to both medical research and clinical diagnostics. (news-medical.net)
METHODS: The clinical data of patients who developed ADV infection diagnosed by mNGS after Haplo-HSCT between January 2019 and March 2021, recorded in three transplantation centers, were retrospectively analyzed. (bvsalud.org)

Thus, accurate diagnostic methods are essential for differentiating the infections. (cdc.gov)

Technical aspects of tissue culture fluorescent antibody technique. (microbiologyresearch.org)
Improved method for the purification of hog cholera virus grown in tissue culture. (microbiologyresearch.org)

Smooth-muscle antibodies were first discovered in 1965 by Johnson et al when they demonstrated that antibodies in the sera of patients with chronic liver disease were able to bind to the smooth muscle of rat stomachs. (medscape.com)

But first, we needed a way to validate that these particles were displaying the SARS-CoV-2 spike protein correctly, and we realized that our DNA-patterning technique would allow us to do this and other exciting experiments in a very efficient way. (sflorg.com)

Shown here, DNA-directed patterning is used to test whether neutralizing antibodies interfere with the ability of ACE receptor to bind with spike-liposomes that have been created using spike protein from two different variants of the SARS-CoV-2 virus. (sflorg.com)

Occasionally, the leptospires can be demonstrated in the fetal tissues (kidney) by sliver stains, or fluorescent antibody techniques. (dvm360.com)
Kozminsky realized that the DNA-printing technique, which was originally developed by the Sohn Lab to "print" different types of cells into patterns that model biological tissues, could also be used to quickly verify that the spike-liposomes were presenting the SARS-CoV-2 spike protein correctly. (sflorg.com)

Therefore, care should be taken to avoid direct exposure of light (especially ultraviolet light) and contact with other compounds in the storage of fluorescent materials. (medicilon.com)

The section was costained with anti-mouse Podoplanin Antibody (clone PMab-1) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse CD31 Antibody (clone MEC13.3) Alexa Fluor® 594 at 5 µg/mL (orange), and anti-Tubulin β 3 (TUBB3) Antibody (Clone TUJ1) Alexa Fluor® 647 at 5 µg/mL (magenta). (biolegend.com)
The growing demand for multiplex western blotting has driven the development of many new fluorescent dyes (such as Alexa Fluor Plus conjugates). (thermofisher.com)

There is a high prevalence of CT IgG antibody in Brazilian pregnant and infertile women, but we found a low prevalence of positive PCR in the urine samples. (ufrgs.br)
The solid-phase antibody is reacted with a urine specimen. (cdc.gov)
1992). Although the method was used for rat urine, it would be expected that human urine could also be utilized. (cdc.gov)
Although no literature citations were found, it would seem that formate in urine and blood could be determined by a method based on ion chromatography (IC). (cdc.gov)

These advantages have allowed chemiluminescence to become the detection method of choice in most protein laboratories. (thermofisher.com)

The direct fluorescent antibody (DFA) method allows detection of virus within 2-3 hours, but is labor intensive and requires considerable experience. (quidel.com)

The technique was established in the 1950s, and over the decade's many advancements have been achieved in its methodology and equipment. (news-medical.net)
The analytical methods are described in the Description of the Laboratory Methodology section. (cdc.gov)

Various methods have been employed in the epidemiological assessment of malaria. (who.int)
The IFA test was shown to be useful to study the antibody levlels of blood donors from different areas in Sri Lanka, to observe the production and persistence of malarial antibodies in man and rhesus monkeys and for epidemiological assessment of malaria in Sri Lanka. (who.int)
The follow up of eight malaria patients for persistence of antibodies showed that the range of duration was between 6 weeks and 10 weeks. (who.int)

Anatomy3

Organisms13

Diseases11

Chemicals and Drugs21

Analytical, Diagnostic and Therapeutic Techniques and Equipment23

Phenomena and Processes7

Information Science1

Health Care3

Humans3

Antigen13

Immunofluorescence10

Monoclonal antibody1

Reactivity1

Assay6

Proteins8

Detect9

Fluorescein4

ELISA2

Assays2

Immunoassay6

Flow cytometry2

Titers3

Highly multiplexed2

Fluorescence8

Specific antigens2

Serum5

Nucleic acids2

Reagents2

Sensitivity3

Polymerase Chain Re1

Localization1

Secondary1

Rabies3

Microscopy techniques1

Specificity1

Cellular1

Bound1

Isolates1

Molecules2

Immunoassays1

Covalently2

Chemiluminescent3

High throughput2

Measurement3

Substances1

Serological1

Green fluores1

Brain1

Concentration2

Quantification1

Detection methods1

Affinity1

Herpes virus1

Clinical2

Infections1

Tissue culture2

19651

Particles1

Bind1

Tissues2

Compounds1

Alexa Fluor2

Urine4

Protein1

Direct1

Methodology2

Malaria3