Methods cell microdissection. Medical search. Frequent questions

Dr. Lubman's team used a combination of immunostaining with laser capture microdissection (immuno-LCM). (genengnews.com)

4. Evaluate the expression of p16, PTEN and CCDN1 mRNA in parafin embedded tissue, obtained by laser microdissection. (fapesp.br)

NEW YORK - Researchers at the University of California, Santa Cruz have pioneered a method for repeatedly sampling messenger RNA (mRNA) from single cells without killing the cells in question. (genomeweb.com)
The method, which was recently published in the journal PLoS One , relies on automated nanopipetting from slide-mounted, intact tissue and mRNA capture using a just-launched magnetic mRNA purification and sequencing library preparation platform from nanoparticle assay developer Nvigen. (genomeweb.com)
In the proof-of-concept study, Pourmand's group aspirated 22 cells within a breast cancer tissue slice and selectively captured the mRNA using Nvigen's proprietary antibody-coated magnetic nanoparticles. (genomeweb.com)
Hundreds of genes were sequenced from each cell at each aspiration, and the genes sequenced showed high correlations from one sampling to the next, indicating that the small amount of mRNA withdrawn from each cell did significantly affect gene coverage. (genomeweb.com)
Where most scRNA-seq methods involve killing the cell from which messenger RNA is taken, the UCSC method leaves cells alive for further sampling, taking only between 1 and 5 percent of the cell's mRNA per sampling event. (genomeweb.com)
Upon activation, a light-activated biotin tag is used to bind the poly-A tails of mRNA, capturing mRNA within live cells and intact tissues in a relatively noninvasive manner. (genomeweb.com)
Neurone, astrocyte, and endothelial cell-enriched mRNA, obtained by immuno-laser capture microdissection of temporal cortex (Brodmann area 21/22) from 6 cases with self-reported T2D in the Cognitive Function and Ageing Study neuropathology cohort, and an equal number of age and sex-matched controls, was assessed by microarray analysis. (biomedcentral.com)
Targeted disruption of the gene encoding D4Rs reduces the amplitude of the contrast sensitivity rhythm by reducing daytime sensitivity and abolishes the rhythmic expression of Npas2 and Adcy1 mRNA in the ganglion cell layer (GCL) of the retina. (jneurosci.org)
However, the inclusion of neighbouring cells dilutes the syncytium-specific mRNA population. (biomedcentral.com)
Oligonucleotide microarray technology is a powerful application to measure genome-wide changes in mRNA expression levels in various tissue samples or cell lines. (biomedcentral.com)

Laser capture microdissection (LCM), also called microdissection, laser microdissection (LMD), or laser-assisted microdissection (LMD or LAM), is a method for isolating specific cells of interest from microscopic regions of tissue/cells/organisms (dissection on a microscopic scale with the help of a laser). (wikipedia.org)
Laser-capture microdissection (LCM) is a method to procure subpopulations of tissue cells under direct microscopic visualization. (wikipedia.org)
IR laser provides a more gentle approach to microdissection. (wikipedia.org)
A fifth ultraviolet laser based technology uses special slides coated with an energy transfer coating which, when activated by the laser pulse, propels the tissue or cells into a collection cap. (wikipedia.org)
The laser cutting width is usually less than 1 μm, thus the target cells are not affected by the laser beam. (wikipedia.org)
Even live cells are not damaged by the laser cutting and are viable after cutting for cloning and reculturing as appropriate. (wikipedia.org)
NCEZID scientists used a technique known as laser microdissection to pinpoint the specific cells where Zika virus might be persisting and replicating . (cdc.gov)
This involves looking under the microscope and guiding a laser to cut away specific cells from areas of the brain and placental tissues, so scientists could measure the amount of Zika virus genetic materials (RNA) in specific cells. (cdc.gov)
Another method called Geo-seq combines scRNA-seq with laser capture microdissection. (genomeweb.com)
Instead, we used laser microdissection to excise putative progenitor cells and control cells from their in situ locations in cryosections and characterized the molecular properties of these cells. (frontiersin.org)
To study epigenetic changes and genes that are expressed differently in different cell lineages-such as cancer cells versus normal cells, or the two types of epidermal cells in Arabidopsi s roots-typically requires laser capture microdissection (LCM) or fluorescence-activated cell sorting (FACS). (the-scientist.com)
LCM uses a laser and a microscope to literally flip individual cells out of a tissue into a container. (the-scientist.com)
Using a workflow combining laser microdissection and single-cell mass spectrometry, the researchers identified more than 1,700 proteins per liver cell. (genomeweb.com)
Alternatively, gene expression patterns can be resolved using laser capture microdissection (LCM), which samples tissues on a grid. (scienceboard.net)
The kit contains reagents for the effective cell lysis of isolated single cells or small clusters of cells, for example from laser-capture microdissection platforms. (qiagen.com)
Then as a clinical lecturer in Liverpool while training in paediatric infectious diseases his research took a more basic approach, to understand the mechanisms of his PhD observations: developing in vitro methods and using laser capture microdissection to examine specific cell populations using transcriptomics in patient samples. (gla.ac.uk)
Amyloid plaques and neighbouring non-plaque tissue were microdissected from human brain sections using laser capture microdissection and label-free LC-MS was used to quantify the proteins present. (researchsquare.com)
Falk Schlaudraff (Institute of General Physiology of the University of Ulm, Germany) used modern laser microdissection methods to isolate and analyze cells from post mortem tissue specimens taken from M. Parkinson patients in order to gain molecular insight into the disease. (leica-microsystems.com)
As part of his Ph.D. he analyzed the gene expression of human dopaminergic neurons, which he isolated from post mortem midbrain tissue of Morbus Parkinson patients and control subjects using a laser microdissection system from Leica Microsystems. (leica-microsystems.com)
Our analysis, for example the RNA quality of the specimens, showed that the quality of the results is not influenced by the staining process and laser microdissection. (leica-microsystems.com)
A) Left panel: cresylviolet (CV)-stained mouse coronal midbrain section indicating the area of the substantia nigra pars compacta (SNpc) before (left) and after (right) UV -laser-microdissection of the entire area. (leica-microsystems.com)
Here, we describe a set of hierarchical analytical methods to process, combine, and harmonize single-cell, single-nucleus and subsegmental laser microdissection (LMD) transcriptomics with LMD and near single-cell proteomics, 3-D nondestructive and immunofluorescence-based Codex imaging and spatial metabolomics datasets. (biorxiv.org)
We collected 2000 individual cells per batch from FFPE liver slices with laser capture microdissection and used them as test samples. (bvsalud.org)
The growth of the special genomics and transcriptomics market is further accelerated due to the availability of automated sequencing methods like laser capture microdissection technique (LCM) to satisfy the requirement for nucleic acid sequencing of biological specimens. (maximizemarketresearch.com)
To examine the differentiation, mESCs were selectively extracted from the co-culture using laser microdissection and analyzed using real-time reverse transcriptase (RT)-polymerase chain reaction (PCR). (ca.gov)
Micro-sectioning coupled with laser capture microdissection (LCM) offers an opportunity for the isolation of feeding sites from heterogeneous cell populations. (biomedcentral.com)
Laser microdissection microscopy has become a rising tool to assess gene expression profiles of pure cell populations. (biomedcentral.com)
From Eye to Insight is therefore fitting perfectly to Leica Microsystems and in particular to laser microdissection. (leica-microsystems.com)
Brain Research: Collect pure starting material for proteomics - Improve your workflow with Laser Microdissection - Many brain diseases result from protein malfunction, misfolding and agglutination. (leica-microsystems.com)
The main topic during this course was how to apply laser microdissection in neuroscience. (leica-microsystems.com)
During the first Leica Workshop in Brazil, at the Centro de Energia Nuclear na Agricultura/USP (CENA), the participants learned how to prepare samples for laser microdissection (LMD) using a cryotome. (leica-microsystems.com)
Gene expression in rat lacrimal gland duct cells collected using laser capture microdissection: evidence for K+ secretion by duct cells. (cdc.gov)
Laser Capture Microdissection enabled directed cancer and stromal cell isolation. (thevirusproject.org)
Other examples of specimen requiring amplification for genome-wide characterization of gene expression include purified populations of cells obtained by either flow cytometry, laser capture microdissection, breast ductal or bronchial lavage, or microendoscopy. (biomedcentral.com)
Methods The miR-224-5p expression in CRC patient tissues and cell-derived exosomes was measured by laser capture microdissection and qRT-PCR, respectively. (besjournal.com)
White matter perivascular and intrameningeal immune infiltrates, including B cell follicle-like structures, were isolated from brain sections using laser capture microdissection. (biomedcentral.com)
[ 11-13 ] By contrast, we discovered, using laser capture microdissection and mass spectrometry, that keratin 17 (K17) could predict the overall survival of patients with cervical squamous cell carcinoma more accurately than grade, stage, or any other clinicopathologic features. (medscape.com)

The goals for advances in flow cytometry are clear: measure as many relevant target molecules per cell as quickly as possible. (nature.com)
Fluorescence and isotope tagging are the principal means for measuring antibody binding to cells in flow cytometry. (nature.com)
Flow cytometry was used to detect cell cycle and apoptosis. (besjournal.com)
Cell cycle and apoptosis were analyzed by flow cytometry. (bvsalud.org)

The utility of longitudinal data in single-cell spatial transcriptomics is well known and numerous methods attempt to address it. (genomeweb.com)
Gene expression quantification of the sections is implemented using spatial transcriptomics by reoriented projections and sequencing (STRP-seq), a method that combines the sampling strategy presented above with a customized, low-input RNA-seq protocol based on single-cell tagged reverse transcription sequencing (STRT-seq) chemistry. (scienceboard.net)
The growth of the segment is attributed to the high adoption of the transcriptomics segment as compared to the genomics segment for the analysis of single-cell transcriptomics. (maximizemarketresearch.com)
As library enrichment methods have improved, dual-species transcriptomics studies expanded to include the study of eukaryote-eukaryote and prokaryote-prokaryote systems. (biomedcentral.com)

Previous molecular characterizations of mammary stem cells (MaSC) have utilized fluorescence-activated cell sorting or in vitro cultivation of cells from enzymatically dissociated tissue to enrich for MaSC. (frontiersin.org)

The membrane-tissue entity now adheres to the cap and the cells on the cap can be used in downstream applications (DNA, RNA, protein analysis). (wikipedia.org)
Under a microscope using a software interface, a tissue section (typically 5-50 micrometres thick) is viewed and individual cells or clusters of cells are identified either manually or in semi-automated or more fully automated ways allowing the imaging and then automatic selection of targets for isolation. (wikipedia.org)
The various technologies differ in the collection process, possible imaging methods (fluorescence microscopy/bright field microscopy/differential interference contrast microscopy/phase contrast microscopy/ etc.) and the types of holders and tissue preparation needed before the imaging and isolation. (wikipedia.org)
Most surgically removed tissue samples contain a low percentage of tumor cells embedded in a dense stroma. (genengnews.com)
I solating specific cell types from a mass of plant or animal tissue is laborious and tricky. (the-scientist.com)
One of the aims of my research was to develop optimized qPCR (quantitative polymerase chain reaction)-based methods to enable valid comparison of the gene expression of promising gene candidates in individual neurons from human post mortem tissue from M. Parkinson patients with the gene expression of the same neurons from healthy control subjects. (leica-microsystems.com)
We developed a qPCR-based platform that could be used to isolate individual cells from native tissue and obtain highly comparable gene expression analyses. (leica-microsystems.com)
Embryonic stem cells hold considerable promise in tissue engineering and regenerative medicine as a source of tissue-specific cells. (ca.gov)
However, use of whole tissue homogenates unavoidably results in averaging of expression levels of different cell types present in the tissue. (biomedcentral.com)
Different spatial biology methods in microscopy, such as multiplex imaging, are helping to better understand tissue landscapes. (leica-microsystems.com)
Twenty-three postmortem snap-frozen brain tissue blocks from 11 patients with progressive MS were selected based on good RNA quality and prominent immune cell infiltration. (biomedcentral.com)

CRC cells secreted extracellular miR-224-5p mainly in an exosome-dependent manner, and then miR-224-5p could be transferred to surrounding tumor cells to regulate cell proliferation in the form of autocrine or paracrine. (besjournal.com)
This review concentrated on the function and mechanism of deubiquitinase system in the proliferation, apoptosis, metastasis and autophagy of tumor cells. (bvsalud.org)

The high diagnostic potential of peptides and proteins from cells, tissues, and biological fluids is unquestionable. (genengnews.com)
Our team was the first to conduct an in-depth molecular comparison of cancer and normal tissues for the presence of cancer stem cells," says David M. Lubman, Ph.D., professor of surgery at the University of Michigan, Ann Arbor. (genengnews.com)
Our method overcomes a common problem of heterogeneity of cancer tissues. (genengnews.com)
Spatially resolved molecular atlases help scientists understand where different types of cells are located in the body and map their gene expression in specific locations in tissues and organs. (scienceboard.net)
Enthesis development has historically been studied with lineage tracing of individual genes selected a priori, which does not allow for the determination of single-cell landscapes yielding mature cell types and tissues. (elifesciences.org)
Expression of DCT was absent or barely detected in retina tissues, various human ocular cells, and major organs. (molvis.org)

The protein expressions of p53 and unc-51 like kinase 2 (ULK2) in CRC cells were detected by western blot. (besjournal.com)

Identification of crucial cancer biomarkers requires analysis of a pure and homogeneous cell population. (genengnews.com)
Effective, reliable use of molecular biomarkers to inform clinical practice requires the standardisation of testing methods and careful assessment of biomarkers' predictive and prognostic value. (ersjournals.com)

Analysis of immunophenotypically defined cell populations markedly increased specificity of liquid chromatography-tandem mass spectroscopy (LC-MS/MS). Over 375 differentially expressed proteins were analyzed by Ingenuity Pathway Analysis (Ingenuity Systems). (genengnews.com)
Of these, single cell RT-qPCR provides combined advantages of sensitivity, specificity, and dynamic range, but is limited by low throughput, high reagent cost, and difficulties in accurately measuring low abundance transcripts (6). (justia.com)

Germinal-center B-cells undergo clonal expansion and activate the process of somatic hypermutation that introduces mutations at a high rate into rearranged immunoglobulin variable genes. (medscape.com)
Germinal-center B-cells with unfavorable mutations are functionally crippled and undergo programmed cell death (apoptosis). (medscape.com)
Low background and comprehensive coverage make this kit highly suited for the analysis of aneuploidy, copy number variations and mutations from single cells and low-input samples. (qiagen.com)
Using mouse, chick or zebrafish models, both test what goes awry when mutations derail migrating neural crest cells in a developing embryo. (stowers.org)
DNA mutations lead to abnormal proteins or missing functional proteins, which can cause cells to multiply uncontrollably and become cancerous. (leica-microsystems.com)
This group of cells first enters the drug tolerance state (DT state) under drug pressure, and gradually acquires stable drug resistance through adaptive mutations in this state. (bvsalud.org)

Due to the PCR-free workflow, this kit is highly suited for the analysis of aneuploidy, copy number and sequence variation in single cells from rare samples. (qiagen.com)

Enriched expression of genes in LRECb was associated with stem cell attributes and identified WNT, TGF-β, and MAPK pathways of self renewal and proliferation. (frontiersin.org)
whereas LREC in suprabasal epithelial layers are enriched for more committed progenitor cells, expressing some genes that are associated with stem cell attributes along with those indicative of cell differentiation. (frontiersin.org)
This technique requires preselection of target genes and sequencing methods with resolution limitations. (scienceboard.net)
912, 2202, and 1227 genes were significantly differentially expressed between cases with self-reported T2D and controls in neurones, astrocytes, and endothelial cells respectively. (biomedcentral.com)
For example, single cell analysis allows for the direct measurement of gene expression kinetics, or for the unambiguous identification of co-regulated genes, even in the presence of de-synchronization and heterogeneity that could obscure population-averaged measurements. (justia.com)
General objective: To evaluate the cell-cycle proteins, the supressor genes and oncogenes in acral lentiginous melanomas. (fapesp.br)
These analyses revealed that mESCs co-cultured with HepG2 cells showed a decrease in pluripotency gene expression concomitant with up-regulation of endodermal genes. (ca.gov)
In more detail, we compared the differentially expressed genes identified after both amplification methods. (biomedcentral.com)
To test this hypothesis, we analyzed expression of viral and cellular genes in brain-infiltrating immune cells. (biomedcentral.com)
Enhanced PCR-based methods were used to investigate expression of 75 immune-related genes and 6 EBV genes associated with latent and lytic infection. (biomedcentral.com)
Genes related to T cell activation, cytotoxic cell-mediated (or type 1) immunity, B cell growth and differentiation, pathogen recognition, myeloid cell function, type I interferon pathway activation, and leukocyte recruitment were found expressed at different levels in most or all MS brain immune infiltrates. (biomedcentral.com)
Comparison of data obtained in meningeal and white matter infiltrates revealed higher expression of genes related to interferonγ production, B cell differentiation, cell proliferation, lipid antigen presentation, and T cell and myeloid cell recruitment, as well as more widespread EBV infection in the meningeal samples. (biomedcentral.com)

we applied Single-cell RNA sequencing (scRNA-seq) to delineate the comprehensive postnatal RC enthesis growth and the temporal atlas from as early as postnatal day 1 up to postnatal week 8. (elifesciences.org)

Join Prof Dr Peter Horvarth , as he presents his work on single cell-based large-scale microscopy experiments. (pragolab.cz)
Shuttle & Find is a unique way to combine imaging and analytical methods of light and electron microscopy . (antisel.gr)

Such development pattern shares an overlapping biological behavior with the growth plate, which is a process of mesenchymal stem cells differentiating into chondrogenic cells and then sequentially into fibrocartilage cells ( Killian, 2022 ). (elifesciences.org)

1) LTS and STS of de novo resected PDAC show clear differences in cancer cell protein activation re-key markers of proliferation and apoptosis (Sox2, Ki67, STAT3, SRC kinase, H3S10). (thevirusproject.org)

1997-2001: Chercheur associé au Department Neuroscience and Cell Biology, Rutgers Univ/UMDNJ, Etats-Unis. (univ-nantes.fr)
Members of the frog Xenopus genus are versatile model organisms used in developmental biology, cell biology, neuroscience, and toxicology research, among others. (cshlpress.com)

In this study, we aimed to develop a method for sample preparation for micro-proteomics on using the FFPE liver samples. (bvsalud.org)
This novel targeting approach includes the use of machine learning models and ultimately enables successful DNA and RNA sequencing, proteomics, lipidomics and targeted electrophysiology measurement on selected cells. (pragolab.cz)

Insights into the biology of stem cells will be gained by confirmation and characterization of candidate MaSC markers identified in this study. (frontiersin.org)
Using the strongest molecular binding partnership in biology to separate different cell types. (the-scientist.com)
QIAseq Single Cell DNA Library Kits UDI are intended for molecular biology applications. (qiagen.com)
11 Department of Molecular and Cell Biology, Verna and Marrs McLean Department of Biochemistry, Alkek Center for Molecular Discovery, Baylor College of Medicine, Houston, Texas. (aacrjournals.org)
Similarly, single cell methods are vital in stem cell research and cancer biology, where isolated populations of primary cells are heterogeneous due to limitations in purification protocols, and it is often a minority cell population that is the most relevant. (justia.com)
To the best of our knowledge, this is the first study that used predicted miRNAs to determine the features that can separate the diseased samples (T2D or AD) from the normal ones, instead of using conventional non-biology-based feature selection methods. (frontiersin.org)
In an interview with Technology Networks, Lundberg discusses the evolution of spatial biology and her career, outlining how spatial insights can provide a deeper appreciation of the complex inner workings of cells. (technologynetworks.com)

We present a method enabling simultaneous, ultra-high throughput single-cell barcoding of millions of cells for targeted analysis of proteins and RNAs. (nature.com)
Although the specific mechanisms underlying the formation of drug tolerant cells (DTCs) remain unclear, various proteins and signalling pathways have been identified as being involved in the formation of DTCs. (bvsalud.org)

The kit includes all reagents required for cell lysis, whole genome amplification using REPLI-g DNA polymerase, enzymatic DNA fragmentation and PCR-free NGS library preparation compatible with Illumina NovaSeq and other NGS instruments. (qiagen.com)
After cell lysis, a high-fidelity MDA reaction is used to amplify microgram amounts of gDNA using the high-fidelity REPLI-g DNA Polymerase. (qiagen.com)
Both of these latter techniques risk changes to the metabolism during cell preparation and barcoding prior to cell lysis- since both approaches, generally, rely on the use of live cells 30 . (nature.com)

During rapid mammary growth in the mouse, label retaining epithelial cells (LREC) appear to retain label by asymmetric distribution of DNA strands, as evidenced by a rapid proliferation index of the LREC ( Smith, 2005 ). (frontiersin.org)
During periods of low mammary proliferation, quiescence of the stem cell population may account for retention of label. (frontiersin.org)
Cell proliferation was measured by CCK8 and EdU assay. (besjournal.com)
Interestingly, ULK2 inhibited CRC cell proliferation in a p53-dependent manner. (besjournal.com)
Furthermore, exosome-derived miR-224-5p partially reversed the proliferation regulation of ULK2 on CRC cells. (besjournal.com)
Conclusion Our findings demonstrate that exosome-transmitted miR-224-5p promotes p53-dependent cell proliferation by targeting ULK2 in CRC, which may offer promising targets for CRC prevention and therapy. (besjournal.com)

LCM technology can harvest the cells of interest directly or can isolate specific cells by cutting away unwanted cells to give histologically pure enriched cell populations. (wikipedia.org)

Single-cell omics provide insight into cellular heterogeneity and function. (nature.com)

Impaired insulin signalling was shared by neurovascular unit cells with, additionally, apoptotic pathway changes in astrocytes and dysregulation of advanced glycation end-product signalling in endothelial cells. (biomedcentral.com)

Dr. Emma Lundberg , professor of bioengineering and pathology at Stanford University and the KTH Royal Institute of Technology, and director of the Cell Atlas portion of the Human Protein Atlas, has been a key figure in such developments. (technologynetworks.com)

This method allowed us to zero in on a small population of cancer stem cells expressing CD24, a specific cell surface biomarker shown to correlate with the development and metastasis of pancreatic cancer," continues Dr. Lubman. (genengnews.com)

Previous work in our laboratory identified keratin 17 (K17) as a specific and sensitive biomarker for high-grade squamous intraepithelial lesions and cervical squamous cell carcinoma (SCC). (medscape.com)
Cervical cancer has multiple histologic subtypes, with squamous cell carcinoma (SCC) accounting for approximately 75% of cases and invasive adenocarcinoma accounting for 10% to 15% of cases. (medscape.com)

Under the agreement, Research Instruments will distribute BioSkryb's single-cell amplification products in Singapore and elsewhere in Southeast Asia. (genomeweb.com)
The QIAseq Single Cell DNA Library Kit UDI includes protocols for unbiased whole genome amplification and for target enrichment of small genomes, such as viral DNA or mitochondrial genomes. (qiagen.com)
In comparison to PCR-based methods of whole genome amplification, the Multiple Displacement Amplification (MDA) technology included in the QIAseq Single Cell DNA Library Kit UDI eliminates PCR duplicates and minimizes GC-bias, ensuring high library diversity and maximum genome coverage. (qiagen.com)
Microfluidic devices employing active valving to position and isolate cells have allowed for the isolation and genome amplification of individual microbial cells (32). (justia.com)
Although these systems provide a high-throughput qPCR readout, they do not address the front end sample preparation and require single cell isolation by FACS or micropipette followed by off-chip processing and pre-amplification of starting template prior to analysis. (justia.com)
This up-regulation is due to gene amplification of c-rel and activation of cell-surface receptors such as CD30, CD40, RANK, and Notch1. (medscape.com)

Directing hepatic differentiation of embryonic stem cells with protein microarray-based co-cultures. (ca.gov)
In this study, we developed a micropatterned co-culture platform for stimulating hepatic differentiation of mouse embryonic stem cells (mESCs). (ca.gov)

Quantum barcoding (QBC) avoids isolation of single cells by building cell-specific oligo barcodes dynamically within each cell. (nature.com)

This dictates cycles of mammary growth, differentiation, lactation, and regression, during which mammary stem cells (MaSC) provide for the lineages of luminal and basal (myoepithelial) epithelial cells in the ducts and alveoli. (frontiersin.org)
Inevitably, there are fundamental and applied questions, such as those relating to transcriptional control of stem cell differentiation, intrinsic noise in gene expression, and the origins of disease, that may only be addressed at the single cell level. (justia.com)
However, realizing this promise requires novel methods for guiding lineage-specific differentiation of stem cells. (ca.gov)
In conclusion, micropatterned co-cultures of mESCs and hepatic cells showed a significant promise in driving stem cell differentiation towards hepatic phenotype. (ca.gov)

NGMLR and Sniffles are long-read sequencing analysis methods to study the longer fragments of DNA, a breakthrough that may yield critical information about how cancer genomes evolve. (agbt.org)

Using this method, samples from patients with diagnosed pancreatic cancer were compared to the healthy controls. (genengnews.com)
The QIAseq Single Cell DNA Library Kit UDI can support up to 384 samples in a single flow-cell lane, increasing sample throughput and lowering sequencing costs. (qiagen.com)
We used the microscale fresh-frozen liver samples or HepG2 cells as control samples. (bvsalud.org)
Compared to single-species differential expression analysis, the design of multi-species differential expression experiments must account for the relative abundances of each organism of interest within the sample, often requiring enrichment methods and yielding differences in total read counts across samples. (biomedcentral.com)

In this study, we investigated the transcriptomic changes in cortical neurones, and associated astrocytes and endothelial cells of the neurovascular unit, in the ageing brain. (biomedcentral.com)
Hypoxia triggers a proangiogenic pathway involving cancer cell microvesicles and PAR-2-mediated heparin-binding EGF signaling in endothelial cells. (lu.se)

While this technique allows scientists to explore single-cell transcriptomes in the context of their natural microenvironment and with clear spatial resolution, it is relatively low throughput with regard to the number of cells that can be analyzed. (genomeweb.com)
Scientists have endeavored to peer into cells and disentangle their complex molecular makeup for hundreds of years. (technologynetworks.com)

In this way, cells of interest are geographically marked by imaging then unwanted cells are cut away via infrared or ultraviolet capture systems. (genomeweb.com)

Transcriptome in vivo analysis (TIVA), for example, enters cell membranes via a cell-penetrating peptide. (genomeweb.com)
Moreover, the device does not allow isolated cells to be washed from the supernatant prior to treatment or analysis. (justia.com)
Accordingly, a goal of microfluidics research has been the development of integrated technology for scalable analysis of transcription in single cells. (justia.com)
Microfluidic systems provide numerous advantages for single cell analysis: economies of scale, parallelization and automation, and increased sensitivity and precision that comes from small volume reactions. (justia.com)
Considerable effort over the last decade has been directed towards developing integrated and scalable single cell genetic analysis on chip (7, 8). (justia.com)
Thus, many of the basic functionalities for microfluidic single cell gene expression analysis have been demonstrated in isolation, including cell manipulation and trapping (9, 10), RNA purification and cDNA synthesis (11-13), and microfluidic qPCR (14) following off-chip cell isolation cDNA synthesis and preamplification. (justia.com)
The critical step of integrating all steps of single cell analysis into a robust system capable of performing measurements on large numbers of cells has yet to be reported. (justia.com)
This paper represents a valuable single-cell level analysis of tendon enthesis development. (elifesciences.org)
The impact of modern scientific methods on the analysis of crime scene evidence has dramatically changed many forensic sub-specialties. (leica-microsystems.com)

We isolated four categories of cells from mammary epithelium of female calves: bromodeoxyuridine label retaining epithelial cells (LREC) from basal (LRECb) and embedded layers (LRECe), and epithelial control cells from basal and embedded layers. (frontiersin.org)
K17 was not detected in the epithelial cells of benign glandular lesions, but groups of cuboidal cells, residing beneath the epithelial layer of benign glands, were frequently positive for K17, especially in cases of microglandular hyperplasia. (medscape.com)

These approaches result in the loss of all histological information pertaining to the in vivo locale of MaSC and progenitor cells. (frontiersin.org)
MaSC/progenitor cells were identified based on their ability to retain bromodeoxyuridine for an extended period. (frontiersin.org)

These cells selectively degenerate as he M. Parkinson disease progresses. (leica-microsystems.com)

Cancer cell exosomes depend on cell-surface heparan sulfate proteoglycans for their internalization and functional activity. (lu.se)
Exosomes reflect the hypoxic status of glioma cells and mediate hypoxia-dependent activation of vascular cells during tumor development. (lu.se)

And next-generation sequencing (NGS) libraries can be used to quantify spatial gene expression patterns that delineate between cell populations. (scienceboard.net)
And, we furtherly performed single-cell spatial transcriptomic sequencing on postnatal day 1 mouse enthesis, in order to deconvolute bone-tendon junction (BTJ) chondrocytes onto spatial spots. (elifesciences.org)

To address this, researchers have developed methods to interrogate the transcriptome of multiple organisms from a single sample. (biomedcentral.com)

The group then analyzed mouse embryos using molecular markers and discovered that precursors of neural crest cells in the brain and spinal cord began dying even before crest cells destined to help build the face could start migrating. (stowers.org)

In the cases where the number of microbial cells are more limited, enrichment methods are needed to derive a meaningful number of reads from the minor organism for statistically robust analyses. (biomedcentral.com)

An in vitro and in vivo comparison among three different human hepatic stem cell populations. (ca.gov)

Printing columns of collagen (I) and fibronectin spots (300 microm diameter), followed by sequential seeding of the two cell types, allowed the positioning of clusters of mESCs adjacent to groups of hepatic cells within the same microarray. (ca.gov)

It is a unique neoplasm in which the malignant cell, the Reed-Sternberg cell (RSC), represents only a small proportion of cells constituting the bulk of the tumor (see the image below). (medscape.com)
In adenocarcinomas, K17 staining was detected in a mean of 33.9% of malignant cells. (medscape.com)

We demonstrate that this method can be applied to multi-modal protein and RNA analyses. (nature.com)
2) For stromal cells, protein activation was largely similar, but a difference in activation of one stromal protein (Mek S298) was seen. (thevirusproject.org)

High-throughput single cell measurement technologies are therefore of interest and have broad applications in clinical and research settings. (justia.com)
He is also interested in the large-scale infrastructure required for modern bioinformatics including storage and access methods for high throughput sequencing data. (agbt.org)

This testing method sheds light on where the virus may replicate and how it moves across the placenta from the mother to the fetus and infects the fetuses' brains. (cdc.gov)

The kit delivers exceptional genome coverage from single cells, populations of cells and low quantities of genomic DNA, including regions with high GC-content. (qiagen.com)

If we sample the cells every 15 minutes, it does not change cell either morphology or activity," he said. (genomeweb.com)
If [intervals are] less than 15 minutes then cells start changing morphology. (genomeweb.com)
However, usefulness of microdissection is often limited by the difficulties in identifying desired cell types by morphology alone. (genengnews.com)

Retention of labeled DNA strands may be attributed to the ability of stem cells to retain the parental DNA strand during asymmetric cell division ( Cairns, 1975 ) or to quiescence of the stem cell population such that the DNA label is not diluted by frequent cell divisions ( Klein and Simons, 2011 ). (frontiersin.org)
A study in Nature suggests epitope editing in donor stem cells prior to bone marrow transplants can stave off toxicity when targeting acute myeloid leukemia with immunotherapy. (genomeweb.com)

La Manno and his team found that the new sampling scheme/algorithm combination significantly outperformed previous in situ hybridization methods. (scienceboard.net)

The nuclear guanine nucleotide exchange factors Ect2 and Net1 regulate RhoB-mediated cell death after DNA damage. (ncsu.edu)
Pancreatic endocrine islet β-cells create and release the two crucial hormones that regulate blood glucose levels: insulin, which acts to lower blood sugar, and glucagon, which raises blood sugar. (frontiersin.org)

Studies of mESC and hepatic cell adhesion preferences revealed that mESCs required fibronectin for attachment, while hepatic cells (HepG2) preferred collagen (I) substrate and did not adhere to fibronectin. (ca.gov)

Anatomy4

Diseases1

Chemicals and Drugs9

Analytical, Diagnostic and Therapeutic Techniques and Equipment30

Phenomena and Processes7

Disciplines and Occupations2

Information Science3

Health Care1

Immunostaining with laser capture microdi1

Obtained by laser microdissection1

MRNA10

Laser37

Flow Cytometry4

Transcriptomics4

Fluorescence-activated cell1

Tissue11

Tumor cells2

Tissues6

Western Blot1

Biomarkers2

Specificity2

Mutations6

Workflow1

Genes12

ScRNA-seq1

Microscopy2

20221

Apoptosis1

Neuroscience2

Proteomics2

Biology7

Proteins2

Lysis3

Proliferation6

Enriched cell populations1

Heterogeneity1

Signalling in endothelial cells1

Pathology1

Correlate1

Squamous cell carc2

Amplification6

Embryonic Stem2

Isolation of Single Cells1

Differentiation4

YIELD1

Samples4

Endothelial2

Scientists2

Ultraviolet1

Analysis9

Epithelial cells2

Progenitor cells2

Selectively1

Exosomes2

Spatial2

Transcriptome1

Molecular1

Microbial cells1

Vitro1

Clusters1

Malignant2

Protein2

Throughput2

Sheds light1

Populations of cells1

Morphology3

Stem2

Situ1

Regulate2

HepG21