Method cell biological assay. Medical search. Frequent questions

Indeed, platelet releasate has previously been shown to induce endothelial cell permeability, endothelial cell chemotaxis, and corneal epithelial cell proliferation in cellular assays [ 7 - 9 ]. (hindawi.com)
We also showed that dose-dependent induction of proliferation was connected with changes in the expression of MKI67, CCND1 and CCNE1 genes in well- and poorly differentiated cancer cells. (spandidos-publications.com)
Our study revealed that Ang II influences EC cells in terms of cancer-related processes, and is responsible for increased proliferation, reduction in apoptosis, increased mobility and modulation of adhesion potential. (spandidos-publications.com)
Therefore, various biologically active peptides such as angiotensin, affecting cell proliferation, have become a new area of study in endometrial cancer research. (spandidos-publications.com)
Recently, a number of studies have shown that Ang II plays an important role in proliferation, invasiveness and migration of tumour cells, alteration of expression of cancer-related genes, as well as in physiological tissue remodelling. (spandidos-publications.com)
The aim of the present study was to functionalize Ti scaffolds with genetically modified elastin-like recombinamers (ELRs), synthetic polymeric proteins containing the elastin epitopes responsible for their mechanical properties and for promoting mesenchymal stem cell (MSC) recruitment, proliferation, and differentiation to ultimately increase scaffold osseointegration. (ibecbarcelona.eu)
Cell adhesion, proliferation, and colonization were enhanced on those scaffolds functionalized with RGD-ELR, while differentiation was promoted on those with SNA15-ELR. (ibecbarcelona.eu)
The combination of both RGD and SNA15 into the same ELR stimulated cell adhesion, proliferation, and differentiation, although at lower levels than those for every single moiety. (ibecbarcelona.eu)
Further investigation on the amount and distribution of RGD and SNA15 moieties in ELRs could improve cell adhesion, proliferation, and differentiation compared to the present study. (ibecbarcelona.eu)
For example, cells analyzed by ELISPOT can be transferred for cloning, proliferation assays, flow cytometry, or other methods of analysis. (bdbiosciences.com)
RIP140 is required for ERα-complex formation, ERα-mediated gene expression, and ERα-dependent breast cancer cell proliferation. (aacrjournals.org)
Cell proliferation analysis revealed that the CHO-k1 and mesenchymal stem cells behaved differently. (biomedcentral.com)
The laser-processed titanium surface increased the proliferation of CHO-k1 cells, reduced the proliferation of mesenchymal stem cells, upregulated the expression of the osteogenic markers, and enhanced alkaline phosphatase activity. (biomedcentral.com)
BACKGROUND AND PURPOSE Interleukin-15 (IL-15) is important in the activation and proliferation of lymphocytic cell populations and is implicated in inflammatory disease. (immune-source.com)
and also potently inhibits IL-15 dependent proliferation of cells expressing IL-15R? (immune-source.com)
DISC0280 also inhibited the IL-15 dependent proliferation of M-07e cells that only express IL-15R? (immune-source.com)

Furthermore, POGLUT2 knockdown of breast cancer cells was established, followed by in vitro biological function assays and in vivo tumor growth study. (frontiersin.org)
Element's complete suite of CMC testing services include in vitro potency testing, in vitro cellular uptake, MTS assays, permeability studies and large molecule characterization, as well as IND/BLA submission support and regulatory consulting. (element.com)
The enzyme-linked immunospot (ELISPOT) assay is a powerful tool for detecting and enumerating individual cells that secrete a particular protein in vitro. (bdbiosciences.com)
The compounds according to this invention show antiproliferative activity and inhibit the growth of tumor cells in vitro and in vivo. (justia.com)
MATERIALS AND METHODS: We established the in vivo pica rat model and the in vitro small intestinal epithelial cell (IEC-6 cell) injury model by cisplatin challenge. (bvsalud.org)
A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. (ctsicn.org)
Antimycotic perfusion through Candida biofilms was demonstrated by a modification of a simple in vitro diffusion cell bioassay system. (who.int)
To understand the fundamental connections between particle properties and human and environmental toxicology we study toxicological effects on all levels, from single cells to ecosystems using various in-vitro, ex-vivo, and in-vivo methods. (lu.se)
establish mechanisms of action by studying the effects of the same chemicals in experimental animals and on human cells in vitro, allowing for a better prediction of human carcinogenicity and assessment of carcinogenic mechanisms. (who.int)

The range of available testing services include cell-based potency assays, bioassays and binding assays, as well as physicochemical and structural analyses, enabling customers to benefit from a fully integrated package. (manufacturingchemist.com)
Reliable potency assays are arguably the most important cell-based bioassays, as these assessments are needed to characterize the ability of a drug to drive biologic activity in accordance with the intended therapeutic function, per ICH Q6B for biological drug substances and products. (element.com)

BD Biosciences tools use multiple methodologies including flow cytometry , bioimaging and microscopy (for live and fixed cell analysis) as well as ELISA , IHC , western blot and spectrofluorometry. (bdbiosciences.com)
During apoptosis, PS translocates to the outer leaflet of the plasma membrane and can be detected by flow cytometry and cell imaging through binding to fluorochrome-labeled annexin V when calcium is present. (bdbiosciences.com)

Quantitative activation suppression assay to evaluate human bone marrow-derived mesenchymal stromal cell potency. (ctsicn.org)
The behavior of mesenchymal stem cells from an umbilical cord vein was evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, a mineralization assay, and an alkaline phosphatase activity assay and by carrying out a quantitative real-time polymerase chain reaction for osteogenic markers. (biomedcentral.com)

Biological Assay" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (ctsicn.org)

The natural cytotoxicity receptor NKp44 (NCR2, CD336) is expressed on the majority of porcine NK cells ex vivo without stimulation. (usda.gov)
In particular, our ERC Synergy project DECODE will pioneer the systematic perturbation of hundreds of key regulators in vivo , combined with single-cell profiling to comprehensively phenotype such mutants. (embl.org)
In vivo clinical assays are still inexistent but there are that show different ways of making a complete dental element in a laboratory in animal models. (bvsalud.org)

The company provides a comprehensive range of testing for innovator molecules available either as off-the-shelf assays or custom assay development for more complex proteins. (manufacturingchemist.com)
If you need advanced characterization of biologic drug molecules, potency assay development, cellular response data, lot release support, or more, Element's cell culture expertise is at your disposal. (element.com)
Development of new or improved laboratory/experimental techniques, instruments, or supporting software that measure the location and dynamics of molecules in situ, and organelles, cells, or tissues on the nanometer and micrometer length scales. (nih.gov)
Although originally developed for analyzing specific antibody-secreting cells, the assay has been adapted for measuring the frequencies of cells that produce and secrete other effector molecules, such as cytokines. (bdbiosciences.com)
A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. (lookformedical.com)
In the biological sciences the suffix -omics is used to refer to the study of large sets of biological molecules. (who.int)
Through various omic technologies it is now possible to interrogate large sets of biological molecules. (who.int)

The OPs inhibit acetylcholinesterase (AChE) in red blood cells (RBCs) and pseudocholinesterase (PChE) in serum or plasma. (cdc.gov)
It was necessary to perform serum assays within 48 hours of patient exposure since malathion is quickly hydrolyzed in human tissues. (cdc.gov)
Sample K was from a person known to solid CO , asked to store serum at -20°C and viruses at 2 show nonspecific assay reactivity for antibodies to H5. (cdc.gov)
BIO-MPM-1 Medium is a multi-purpose, serum-free medium for culture of a wide variety of anchorage-dependent cells. (bioind.com)
Study on the interaction of some (E)-2-benzyldenebenzosuberone derivatives with serum albumin by UV-Vis method, inhibitory effect on topoisomerase. (syncsci.com)
or without DISC0280 and assessing changes in lymphocytic cell populations and serum cytokines was utilized. (immune-source.com)
This binding inhibitor can be present both in the serum and in body tissues and might inhibit uptake of thyroid hormones by cells or prevent binding to nuclear T3 receptors, thus inhibiting the action of the hormone. (medscape.com)
red blood cell folate and serum folate will be evaluated using the World Health Organization-recommended microbiologic assay. (cdc.gov)

The Er1 F/− EV cargo is taken up by recipient cells leading to an increase in insulin-independent glucose transporter levels, enhanced cellular glucose uptake, higher cellular oxygen consumption rate and greater tolerance to glucose challenge in mice. (nature.com)
This leads to the loss of cellular structure and function, and ultimately results in cell death. (bdbiosciences.com)
From determining candidates and making initial investment decisions to lot release and stability testing, cellular assays are critical throughout biologic drug development. (element.com)
Leverage the experience and expertise of our team of skilled cell biologists to design cellular assays that carefully assess biologic activity to produce valuable data for product efficacy analysis, internal decision-making, and regulatory processes. (element.com)
Our scientists are adept at rapidly executing stage-appropriate, custom cell-based bioassay development and validation, in addition to other more routine cellular assays to assess biologic drugs robustly and accurately, regardless of modality, mechanism of action and target. (element.com)
This includes gene, protein and metabolic networks, cellular architecture and intracellular dynamics, cell communication and motility, cell division and differentiation, tissue formation and organogenesis, tissue and organ functions, changes in population characteristics as a consequence of interaction of organisms with their physical environment, with individuals of their own species, and with organisms of other species. (nih.gov)
A new mapping method let researchers discover new links (gray lines) between two groups of plant proteins (yellow and blue) that have a common structure (the BBX domain), suggesting many different combinations of interactions, rather than a few, are involved in coordinating cellular programs like flowering time and circadian rhythm. (salk.edu)
Thus, HERA is a novel cellular assay that can be used to predict how FcRn-binding proteins are rescued from intracellular degradation. (nature.com)
The session on super-resolution microscopy will explore its emergence from basic research applications towards exciting new applications in industrial scale screening of biological events at unparalleled sub-cellular scales. (slas.org)
Good needle aspiration cytology Smear showed diffusely cellular stroma, bedding of epithelial cells and multiple bare nuclei with no atypia. (columbiagypsy.net)
The laser-treated titanium surface modulated cellular behavior depending on the cell type, and stimulated osteogenic differentiation. (biomedcentral.com)
Oxidant damage to cellular DNA, proteins (including the epigenome), and lipids can occur when reactive oxygen species escape cell antioxidant and repair mechanisms. (who.int)

We demonstrated that this cell line responds to compounds typically used in the field of sGC research and that it represents an innovative and robust assay to screen for sGC modulators with high efficiency and high sensitivity by means of standard luminescence readers. (nih.gov)
Unique strengths of the assay include high sensitivity, high throughput, high content analysis, minimal volume of biological material required, applicability to frozen/thawed biological samples, and compatibility with other assays. (bdbiosciences.com)
This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. (cancerindex.org)
52 Measure focus using a high-sensitivity assay (e.g. (eprf.ca)

Apoptosis is an organized process that signals cells to self-destruct for cell renewal or to control aberrant cell growth. (bdbiosciences.com)
As cells become damaged or are no longer needed, they undergo apoptosis or programmed cell death, a normal physiological process that occurs during embryonic development and tissue homeostasis. (bdbiosciences.com)
Apoptosis controls the orderly death of damaged cells, whereas necrosis occurs as a result of tissue damage, causing the loss of both damaged and surrounding cells. (bdbiosciences.com)
However, some cell types do not display characteristic features of apoptosis. (bdbiosciences.com)
In those cases, multiple aspects of apoptosis might need to be analyzed to confirm the mechanism of cell death. (bdbiosciences.com)
Methods for detecting apoptosis or dead cells (viability) by cell preparation type. (bdbiosciences.com)
With the addition of these new formats, more complex assays can be developed to look at apoptosis within heterogeneous cell subsets. (bdbiosciences.com)
Radio-frequency dose-dependent apoptosis, necrosis and cell death monitored by annexin V-BD Horizon™ V450. (bdbiosciences.com)
Radio-frequency dose-dependent apoptosis, necrosis and cell death monitored by annexin V-BD Horizon™ V450 in pancreatic carcinoma cell lines treated with a low dose of cetuximab targeted gold nanoparticles. (bdbiosciences.com)
Nitric oxide (NO) is a key vertebrate biological messenger, playing an important role in neurotransmission, vascular regulation, immune responses and apoptosis. (abcam.com)
Many tumor drugs act by way of mechanisms such as blockading the mechanism of cell division in the cell, preventing the tumor from being supplied with nutrients and oxygen (antiangiogenesis), preventing metastasis, preventing the reception and the onward transmission of growth signals to the tumor cell or forcing the tumor cell into programmed cell death (apoptosis). (justia.com)

This luminescent assay, which is suitable for both 96- and 384-well plate formats, has been achieved by stably expressing the alpha and beta subunits of a mutated form of sGC in Chinese hamster ovary cells. (nih.gov)
This delivers label-free, non-activated cells suitable for immunologic or cell biological assays. (wikipedia.org)
Jennifer Lawson, Product Manager for Testing at Sartorius Stedim BioOutsource, commented: "With our unrivalled experience in biosimilars testing, combined with our proven expertise in NBE assay development, we have developed a panel of assays suitable for mAb characterisation. (manufacturingchemist.com)
In previous courses, we have guided students in the ablation of a wide variety of genes and helped them design suitable assays for their biological interests. (cshl.edu)
BIO-MPM-1 Medium is suitable for a wide variety of cells. (bioind.com)

The purpose of this chapter is to describe the analytical methods that are available for detecting, measuring, and/or monitoring malathion, its metabolites, and other biomarkers of exposure and effect to malathion. (cdc.gov)
The intent is not to provide an exhaustive list of analytical methods. (cdc.gov)
Many of the analytical methods used for environmental samples are the methods approved by federal agencies and organizations such as EPA and the National Institute for Occupational Safety and Health (NIOSH). (cdc.gov)
Other methods presented in this chapter are those that are approved by groups such as the Association of Official Analytical Chemists (AOAC) and the American Public Health Association (APHA). (cdc.gov)
Additionally, analytical methods are included that modify previously used methods to obtain lower detection limits and/or to improve accuracy and precision. (cdc.gov)
Several analytical methods have been developed to determine AChE and PChE in blood. (cdc.gov)
Analytical methods for the detection of metabolites of malathion in urine are more sensitive than those used to determine AChE and PChE in blood. (cdc.gov)
Table 7-1 summarizes the analytical methods used to detect malathion and its metabolites as well as cholinesterase activity in biological tissues and fluids. (cdc.gov)
Thus, pharmaceutical companies can now work with one contract partner from cell line development to qualification and validation of analytical and biological product characterisation assays. (manufacturingchemist.com)
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers. (lookformedical.com)

This allows drug developers to fast-track complex bioassay development using our platform methods. (manufacturingchemist.com)
Not only are reliable and accurate methods for determining drug potential and efficacy early in development essential, but cell-based bioassay development and biological assay validation must be accomplished quickly, given the highly competitive biologic industry, particularly for cell and gene therapy modalities. (element.com)

Results obtained from the methods are generally comparable, but differ widely in accuracy, sensitivity, and precision. (cdc.gov)
Because of the increase in sensitivity, the methods are capable of revealing exposures that may be insufficient to bring about a toxic response. (cdc.gov)
Based on the sandwich ELISA, the ELISPOT assay derives its specificity and sensitivity by employing high affinity capture and detection antibodies and enzyme-amplification. (bdbiosciences.com)
The sensitivity of the assay lends itself to measurement of even very low frequencies of cytokine producing cells (e.g., 1/300,000). (bdbiosciences.com)

Proteomic approaches have proven powerful at identifying large numbers of proteins, but there are fewer reports of functional characterization of proteins in biological tissues. (hindawi.com)
Increased levels of POGLUT2 were found in multiple types of cancer tissues and cell lines. (frontiersin.org)
The expression levels of JNK, p-JNK, Bax, Bcl-2, caspase-9, caspase-3 and GSDME in GI tissues and IEC-6 cells were determined by WB. (bvsalud.org)

Half-life assay by proteomic approach can be a database repository, a biological assay, or a tool for biological study. (zeemly.com)
The assignment comprises transcriptomic (bulk and sc-mRNA-seq), epigenomic (ATACseq, CUT&RUN), proteomic (Olink assays), and compositional, metabolic and immunophenotypic profiling (high dimensional cytometry). (lu.se)

The most widely used is a colorimetric assay developed by Ellman et al (1961). (cdc.gov)
Nitric Oxide Assay Kit (Colorimetric) ab65328 provides an accurate, convenient measure of total nitrate/nitrite in a simple two-step process. (abcam.com)
This product is manufactured by BioVision, an Abcam company and was previously called K262 Nitric Oxide Colorimetric Assay Kit. (abcam.com)

Isolated and purified cells are being deployed for basic research as well as diagnostic applications. (wikipedia.org)
This can be used for i.e. drug screening, diagnostic assays, immobilization and interaction studies. (wikipedia.org)
Also, growing demand for rapid and precise measurement of biomolecules, increased use of Immunofluorescence Assay for diagnostic and research purposes, increased R&D activities and advanced technology are the key factors for the market to grow. (medgadget.com)

BD Biosciences also offers a range of tools for caspase activity assays from individual fluorogenic peptide substrates and inhibitors, to kits, to ready-to-use assay plates. (bdbiosciences.com)
The individual synthetic tetrapeptide substrates, together with the caspase inhibitors and active caspase enzymes, offer flexibility in the experimental design of a caspase activity assay. (bdbiosciences.com)

Although mass spectrometry is an effective tool for many different protein investigations, its use is primarily limited to qualitative methods. (zeemly.com)

Its effect and effectiveness appear to be highly connected with the differentiation status of the cancerous cells, as Ang II appears to play a crucial role in the early and late stages of malignant transformation. (spandidos-publications.com)
It is theoretically possible to develop a surface with characteristics optimized for cell attachment, growth, and/or differentiation. (biomedcentral.com)

Biological assays and gene expression analysis were performed on three cell lines: ISH, MFE-296 and MFE-280. (spandidos-publications.com)
Including gene expression data (ref), network modeling (ref), DNA copy number data (ref), and massively parallel reporter assays(ref). (lu.se)

High-affinity zinc uptake system protein mutant ( znuA mutant) showed reduced growth in zinc chelated medium, and failed to replicate in HeLa cells and mouse bone marrow-derived macrophages. (go.jp)

The methods we develop allow us to exploit large and high-dimensional omics data to identify disease signatures and pinpoint causal drivers. (embl.org)
We are also actively advancing multi-omics profiling methods in single cells and applying spatial omics to a variety of questions in fundamental biology, as well as in disease contexts. (embl.org)
Illustration of a statistical method for integrating multiple omics datasets. (embl.org)
Multi-omics factor analysis (MOFA) is a computational framework for unsupervised discovery of the principal axes of biological and technical variation when multiple omics assays are applied to the same samples or cells. (embl.org)
Their application has become feasible in recent years due to an increase in the resolution and throughput of omics-based assays along with a lowering of cost. (who.int)
If omics-based assays were applied with appropriate study designs, thorough validation of the markers, and careful interpretation of study results then a bioinformatics database could be built of the human response to different chemical exposures and associated chronic diseases. (who.int)

Specific activation conditions and incubation times will vary, depending on cell type, kinetics, and cytokine of interest. (bdbiosciences.com)

The IEC-6 cell viability was detected by CCK-8. (bvsalud.org)

Sartorius Stedim Biotech has launched services for new biological entities NBE, product characterisation. (manufacturingchemist.com)
These fully customizable methods and data packages for monoclonal antibody characterisation complement BioOutsource's and Sartorius Stedim Cellca's existing innovative offer for biologics. (manufacturingchemist.com)
Biological Industries is now part of the Sartorius group. (bioind.com)

The power of Xenopus can be leveraged when live-cell fluorescence imaging is combined with microsurgery, grafting, and dissociated cell culture. (cshl.edu)
Fluorescence-based surface markers can also be used to identify other factors of importance to the line of cells being produced, like the location of proteins on the cell, for example. (techcrunch.com)

After Ang II treatment, poorly differentiated endometrial cancer cell line acquired a mesenchymal phenotype, which was characterized by induced expression of EMT-related genes (VIM, CD44, SNAI1, ZEB1 and ZEB2). (spandidos-publications.com)
Human bone marrow mesenchymal stem cells (hBM-MSCs) are the most commonly used cells. (biomedcentral.com)

The objective of this study was to evaluate this fluorescent assay as a tool to measure ChE activities in biological specimens collected from humans and wildlife. (cdc.gov)

Evaluation of a fluorescent method for measuring cholinesterase activity in mammalian blood and tissue. (cdc.gov)
In addition, students can combine these techniques with explant and transplant methods to simplify or test tissue level interactions. (cshl.edu)
Expression, gene mutation and amplification, methylation, and copy number alteration (CNA) of POGLUT2 were evaluated using The Cancer Genome Atlas (TCGA), Cancer Cell Line Encyclopedia (CCLE), and Genotype-Tissue Expression (GTEx) databases. (frontiersin.org)
With a dedicated BSL-2 facility and years of cell and tissue culture experience, our knowledgeable team can work with any cell type in both monolayer and suspension formats, including different immortalized cell lines (such as MDCK), primary cells, and tissue samples (such as brain, blood, milk and more). (element.com)
For example, T-cells (and other cells in our immune system) use mechanical forces to explore their environment, by pulling or pushing the other cells or tissue surfaces. (hu-berlin.de)
The protein complement expressed by an organism's genome, or in the case of multicellular organisms, the protein complement produced by tissue or differentiated cells, has been characterized as the proteome. (zeemly.com)
Meanwhile, there is a collective effort of scientists to create techniques that allow a natural tooth (Bio-tooth) to be created, using different cell populations and tissue engineering techniques. (bvsalud.org)

The primary metabolites found in biological fluids following exposure to malathion are malathion dicarboxylic acid (DCA) and malathion monocarboxylic acid (MCA). (cdc.gov)
We study the interaction between proteins in biological fluids as well as single proteins to nanomaterials of different kinds. (lu.se)

Transformation of znuA mutant with a shuttle vector pBBR1MCS-4 containing znuA gene restored the growth in zinc chelated medium and intracellular replication in HeLa cells and macrophages to a level comparable to that of wild-type strain. (go.jp)
The effects of 3, 3', 4, 4', 5-pentachlorobiphenyl (PCB-126), which is the most toxic congener of coplanar polychlorinated biphenyls (Co-PCBs), on intracellular accumulation and transepithelial transport of vinblastine were examined in porcine kidney cells, LLC-PK1, and its transformant cells expressing human P-glycoprotein (LLC-MDR1). (go.jp)
Since intracellular annexin V is also exposed if the plasma membrane is compromised, a membrane-impermeant dye such as 7-AAD is commonly used to distinguish between apoptotic and dead cells to exclude the dead cells. (bdbiosciences.com)
Xenopus is increasingly being used as imaging test-bed to investigate the roles of cytoskeleton and intracellular trafficking in cell biological and morphogenetic contexts. (cshl.edu)

This webinar will present a survey of high-throughput microscopy and image analysis approaches to interrogate cell models for mechanistic and phenotypic inquiries. (innovationtoronto.com)
Computational biology applications should develop new approaches, algorithms, and mathematical and numerical methods for an integrative understanding of biomedical systems and could span temporal and spatial domains. (nih.gov)
Of interest are development of computational and mathematical algorithms and tools, modeling techniques and approaches for understanding the complexity of biological systems, and utilization of big datasets and data science methods for model construction. (nih.gov)

POGLUT2 was mainly expressed in stromal cells as verified by StromalScore, ESTIMATEScore, ImmuneScore, and Tumor purity, and POGLUT2 was positively correlated with cancer-associated fibroblasts, macrophages, monocytes, and neutrophils in the tumor microenvironment. (frontiersin.org)

The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. (cancerindex.org)
Rapidly growing breast mass could hardly ever become metastasis GS-1101 kinase activity assay from rhabdomyosarcoma, leukaemia, lymphoma, primitive neuroectodermal tumours, Ewing sarcoma, malignant melanoma and renal cell carcinoma.3 However, the size of such masses is not usually as gigantic as in our individual. (columbiagypsy.net)
Treatment The individual underwent endovascular embolisation for bilateral breasts masses, ahead GS-1101 kinase activity assay of surgery, to lessen the vascularity of the large-sized masses. (columbiagypsy.net)
A) Best lateral thoracic artery and its own branches ( em white arrowhead /em ), (B) right inner GS-1101 kinase activity assay mammary artery and its own branches ( em dark arrowhead /em ), (C) postembolisation of the proper lateral thoracic artery and. (columbiagypsy.net)

While the standard method, qPCR, allows for quick and cheap microRNA quantification, specificity is limited due to the short lengths of microRNAs and the high similarity between closely related microRNA family members. (lu.se)

Finally, to visualize subcellular and intercellular activities, we will introduce a variety of sample preparation and imaging methods including time-lapse, fluorescent imaging, optical coherence tomography and confocal microscopy. (cshl.edu)
The morphology of IEC-6 cells was observed by optical microscope and scanning electron microscopy. (bvsalud.org)
Novel microscopy methods unravel the mechanical interaction of T-cells with small particles. (hu-berlin.de)
Scanning electron microscopy revealed that C. albicans formed a contiguous biofilm with tightly packed blastospores and occasional hyphae compared with C. parapsilosis and C. krusei, which developed confluent biofilms displaying structural heterogeneity and a lesser cell density, after 48 h of incubation on nutrient agar. (who.int)

Recently, a fluorescent based ChE assay kit was marketed for measurement of the purified enzyme (A12217, Molecular Probes, Eugene, OR, USA ( Zhou et al 2000). (cdc.gov)
The course maintains stock mRNAs for targeting fluorescent proteins to specific structures for studying cell shape and cytoskeletal dynamics but students are encouraged to bring or suggest additional tools, including fluorescent biosensors, tension-sensors, etc. (cshl.edu)

Rather, the intention is to identify well-established methods that are used as the standard methods of analysis. (cdc.gov)
Samples A and L were identical duplicates from a person pseudotype assay and was excluded from analysis. (cdc.gov)
Additional methods include mRNA in situ hybridization and protein immunohistochemistry as well as basic bioinformatic techniques for gene comparison and functional analysis. (cshl.edu)
MRFR is the Leading Brand in The Research Company who Recently Published Immunofluorescence Assay Market Research Reports which includes Study of growth, Regional Analysis, Top Industry Players Formation, Major Drivers, Upcoming Trends and Forecast to 2023. (medgadget.com)
Element's comprehensive suite of cell-based bioassay services represent a complete tool kit that goes well beyond routine molecular separation and sample analysis. (element.com)
Biostatistics areas of interest include development of advanced statistical techniques and methodologies for design of biological experiments, collection and analysis of the data from those experiments and interpretation of, and inference from, the results. (nih.gov)

While the Ellman assay is simple and inexpensive, the absorbance peak of hemoglobin (415nm) can interfere with detection of its reaction end-product, 2-nitro-5-benzoic acid (405nm). (cdc.gov)
If you are interested in a fluorometric detection kit, please check Nitric Oxide Assay Kit (Fluorometric) (ab65327) . (abcam.com)

Complex biological samples have also been successfully separated in multiple dimensions at the protein level. (hindawi.com)
IBA focusses on two business segments: cell selection and protein purification. (wikipedia.org)
Active caspases can then cleave many proteins, including poly-ADP ribose polymerase (PARP), other caspases and other protein substrates en masse. (bdbiosciences.com)
When cells are positive for a protein interaction, the researchers can use genetic sequencing to figure out what the two proteins interacting are, using new high-throughput DNA sequencing technologies similar to those used for human genome sequencing. (salk.edu)
The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. (cancerindex.org)
10%. In the remaining 10% of children with lecular weight protein found on the surface nephrotic syndrome, it is largely mediated of all nucleated cells which synthesize it. (who.int)

By using machine learning and AI to do the identification based on standard and well-accepted biological assays used by the FDA, the system could move away from human annotation and the variability that introduces into the process of human cell production. (techcrunch.com)

We study the effects on lung cell cultures using e. g. the NACIVT system and the array of characterization instruments of the aerosol lab in close collaboration with Occupational and Environmental Medicine and Lund Bioengineering and Regeneration. (lu.se)

Here, the effects of these alkaloids on cell survival, the activity of superoxide dismutase, catalase, and glutathione peroxidase, factors associated with oxidative stress such as malondialdehyde and dityrosine, carbohydrate-hydrolyzing enzymes alpha-amylase and alpha-glucosidase, insulin secretion levels and glucose uptake ability were investigated. (ijpsonline.com)

1993). There are several methods to measure ChE activity (St. Omer and Rottinghaus 1992, Wilson 2001). (cdc.gov)
In 81 control subjects the levels of folate activity in the red cells were between 80 and 470 mμg/ml (mean 192), and in plasma between 2·1 and 13 mμg/ml (mean 4·9). (bmj.com)
In 26 patients with untreated pernicious anaemia, the red cell folate activity was between 44 and 280 mμg/ml (mean 129) and the plasma level was between 1·6 and 19 mμg/ml (mean 7·5). (bmj.com)
Design, synthesis and antiproliferative activity of some 3-benzylidene-2,3-dihydro-1-benzopyran-4-ones which display selective toxicity for malignant cells. (syncsci.com)

After the addition of 2mM glutamine, BIO-MPM-1 Medium is ready to use for adherent cells. (bioind.com)
Z. Aberman, Adherent cells from placenta and use of same in disease treatment . (bioind.com)
Solid know how of Microsoft office tools is required as well as an experience with cell culture (adherent cells). (lu.se)

High-throughput screening methods provide efficient measurement of the effects of agents or conditions in biological or chemical assays. (innovationtoronto.com)

Element's biologics laboratory has extensive experience working with most biologic drug modalities, including peptides , monoclonal antibodies (mAbs) , proteins/enzymes , antibody-drug conjugates (ADCs), lentivirus/adeno-associated virus gene therapies , cancer vaccines, cell therapies and beyond. (element.com)
For example, there were no T cell subsets and antibodies were all polyclonal. (cityofhope.org)

A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million. (lookformedical.com)
Sessions will cover the process of translating biological insight into tractable assays, efficient tool compounds and biologicals, and methods for generating attractive starting points for drug discovery programmes. (slas.org)
In silico molecular modeling methods, such as docking can aid in the drug discovery process by ascertaining the binding affinities of existing and hypothetical compounds towards Pf TrxR and the human isoform of this enzyme. (biomedcentral.com)

We will continue to develop innovative computational methods to analyse data from high-throughput genetic and molecular profiling studies. (embl.org)
The Nilsson lab has a long track-record of developing computational methods and tools to analyze genomics data. (lu.se)
The lab led by Assistant Prof. Camila Consiglio employs high-throughput multiomics technologies and advanced computational methods to explore sex differences in human immunity. (lu.se)

The beta-TC-6 pancreatic cell line was incubated with oleracein E and L at concentrations of 0, 50, 100, 200 and 400 µM and tested separately. (ijpsonline.com)
Hence, these alkaloids have considerable antioxidant and potential hypoglycemic effects on the pancreatic cell line and they could be suggested for future studies in the treatment of diabetes mellitus. (ijpsonline.com)

In order to find new modulators for this important enzyme, an innovative cell-based assay has been developed and optimized for the use in high-throughput screening. (nih.gov)
The development and validation process of a high throughput, high-plex mIF assay to derive meaningful biological insights. (genengnews.com)
This method - called high-throughput combinatorial printing (HTCP) - controls both the printed materials' 3D architectures and local compositions and produces materials with gradient compositions and properties at microscale spatial resolution. (innovationtoronto.com)
LA JOLLA-Salk scientists have developed a new high-throughput technique to determine which proteins in a cell interact with each other. (salk.edu)
Until now, researchers have typically relied on standard high-throughput yeast two-hybrid (Y2H) assays to determine the interactions between proteins. (salk.edu)
This results in a throughput of about 5,000 cells per minute, which is highly efficient compared with manual techniques. (techcrunch.com)
The candidate will apply systems immunology methods to comprehensively profile the human immune system using various high-throughput technologies. (lu.se)

For example, the use of personalized human retinal cells could be transplanted to halt or reverse age-related macular degeneration, which can cause blindness. (techcrunch.com)

Furthermore B-E29 could inhibit the improved NK1 also.1+ cell production induced by administration from the hIL-15/sIL-15R? (immune-source.com)

The method is based on one of the strongest non-covalent interactions in nature, which is the interaction of biotin to streptavidin. (wikipedia.org)

Following activation by agonists such as thrombin, platelets release storage granules and membrane vesicles that contain prothrombotic (e.g., fibrinogen), mitogenic (e.g., platelet derived growth factor), immunomodulatory (e.g., neutrophil-activating peptide 2), and adhesive (e.g., platelet endothelial cell adhesion molecule) proteins. (hindawi.com)

The populations of cells that are stained with annexin V only represent the apoptotic cell populations. (bdbiosciences.com)

IBA has developed an affinity chromatography system for non-magnetic isolation of peripheral blood mononuclear cells, T cells, B cells and other cells of interest. (wikipedia.org)

Conversion of Peripheral Blood NK Cells to a Decidual NK-like Phenotype by a Cocktail of Defined Factors The Journal of Immunology, 190:3939-3948 (2013). (bioind.com)
Human IL-15 injected into Emodin mice caused an increase in NK1.1+ and CD3+ cells in the spleen and peripheral blood and these effects were unexpectedly potentiated by giving DISC0280 with human IL-15. (immune-source.com)
complex also increased progenitor/NK1.1+ cells in the peripheral blood and induced myeloid hyperplasia coincident with expansion of the NK1.1+ population (data not shown). (immune-source.com)

Xenopus is remarkable for studying development and disease, including birth defects, cancer, and stem cell biology. (cshl.edu)
Founded by a team whose backgrounds include physics, stem cell biology and machine learning, Cellino operates in the regenerative medicine industry. (techcrunch.com)
PLOS Biology provides an Open Access platform to showcase your best research and commentary across all areas of biological science. (plos.org)

Anatomy20

Organisms10

Chemicals and Drugs26

Analytical, Diagnostic and Therapeutic Techniques and Equipment16

Phenomena and Processes38

Disciplines and Occupations2

Anthropology, Education, Sociology and Social Phenomena1

Information Science4

Proliferation16

Vitro9

Bioassays2

Flow cytometry2

Quantitative2

MeSH1

Vivo3

Molecules7

Serum8

Cellular12

High sensitivity4

Apoptosis11

Suitable5

Analytical10

Bioassay development2

Sensitivity4

Tissues3

Proteomic2

Colorimetric3

Diagnostic3

Inhibitors2

Mass spectrometry1

Differentiation2

Gene expression2

Bone marrow-derived1

Omics6

Cytokine1

Viability1

Sartorius3

Fluorescence2

Mesenchymal2

Humans1

Tissue7

Fluids2

Intracellular4

Approaches3

Stromal1

Kinase4

Specificity1

Microscopy4

Fluorescent2

Analysis6

Detection2

Protein6

Variability1

Characterization1

Survival1

Activity4

Adherent cells3

Measurement1

Antibodies2

Compounds3

Computational methods3

Pancreatic2

Throughput7

Retinal cells1

Inhibit1

Interaction1

Peptide1

Populations1

Mononuclear1

Peripheral blood3

Biology3