Metabolites mass spectrometry. Medical search. Frequent questions

In this work we present a strategy of using human liver microsomes (HLM) to metabolize known endogenous human metabolites (substrates), producing potentially new metabolites that have yet to be documented. (nih.gov)
Two of them have previously been documented as endogenous human metabolites, the third is an isomer of a microsome-metabolite and the fourth compound has not been previously reported and is also an isomer of a microsome-metabolite. (nih.gov)
This work illustrates the feasibility of using microsome-based metabolism to produce metabolites of endogenous human metabolites that can be used to facilitate the identification of unknowns in biological samples. (nih.gov)
This method measures human metabolites of the pesticides chlorpyrifos, chlorpyrifosmethyl, naphthalene, and carbaryl in urine. (cdc.gov)
Urinary organophosphate pesticides uses azeotropic codistillation of urine, derivitization, gas chromatography-tandem mass spectrometric method (GC-MS/MS). This method can be used to quantify the six dialkylphosphate human metabolites of organophosphate pesticides in urine. (cdc.gov)
Ion response of free rifampin and its human metabolites measured over 14 days by liquid chromatography mass spectrometry in the supernatant of HEL cells. (cdc.gov)

Mass spectrometry imaging (MSI) is a technique used in mass spectrometry to visualize the spatial distribution of molecules, as biomarkers, metabolites, peptides or proteins by their molecular masses. (wikipedia.org)
The purpose of this chapter is to describe the analytical methods that are available for detecting, measuring, and/or monitoring heptachlor and heptachlor epoxide, their metabolites, and other biomarkers of exposure and effect to heptachlor and heptachlor epoxide. (cdc.gov)
BOSTON - Serum levels of several common metabolites appear to be good biomarkers for estimating the severity of traumatic brain injury (TBI) and may serve as predictors of prognosis, Finnish investigators report. (medscape.com)
Decanoic acid and some of these other metabolites that they're looking at are not traditional biomarkers," said Donald Marion, MD, MSc, senior consultant with the Defense and Veterans Brain Injury Center in Silver Spring, Maryland. (medscape.com)
I analyse biomarkers of environmental pollutants in human samples, using mass spectrometry. (lu.se)
A dynamic portrait of metabolic profiles known as "metabolomics" has been used to identify new blood-derived metabolite biomarkers that can distinguish between pancreatic ductal adenocarcinoma and chronic pancreatitis with a high degree of accuracy and at an earlier disease stage than conventional diagnostic testing, researchers say. (medscape.com)

In summary, our results provided a novel perspective to understand the influences of OA administration by investigating endogenous altered levels of lipid metabolites via lipidomics. (nofima.com)
The glucuronidation and elimination of endogenous compounds ( e.g. , bilirubin) and xenobiotics, including HPPH, the major, para -hydroxylated metabolite of the anticonvulsant drug phenytoin (diphenylhydantoin) ( Butler, 1957 ), are catalyzed by a superfamily of membrane-bound isozymes known collectively as UGTs ( Dutton, 1980 ). (aspetjournals.org)
Adaptive pixel mass recalibration for mass spectrometry imaging based on locally endogenous biological signals. (embl.org)
As such, monitoring endogenous CKs and their metabolites might be promising to control fertilizer abuse. (unboundmedicine.com)
Targeted metabolomics is able to accurately identify both the chemical compounds as well as the endogenous metabolites. (ufz.de)
In total, 791 annotated endogenous metabolites were measured by ultra-performance liquid. (lu.se)

Typically, standards are used to help identify metabolites, yet standards cannot be purchased or readily synthesized for many unknowns. (nih.gov)
However, it is not forthright to identify metabolites associated with AD because its metabolic signatures vary with gender and apolipoprotein E (APOE) genotype. (news-medical.net)
To detect and identify metabolites in an unbiased manner or to identify unknown compounds untargeted metabolomic approaches are a valuable tool. (ufz.de)

Analytical methods included gas chromatography/thermal conversion/hydrogen isotope ratio mass spectrometry combined with single quadrupole mass spectrometry as well as liquid chromatography/high accuracy/high resolution mass spectrometry of the hydrolyzed and non-hydrolyzed samples. (uni-koeln.de)
Here we demonstrate that a retention time prediction model can improve metabolite identification on a hydrophilic interaction chromatography (HILIC)-high-resolution mass spectrometry metabolomics platform. (manchester.ac.uk)

Ultra-performance liquid chromatography and time-of-flight mass spectrometry analysis of ginsenoside metabolites in human plasma. (uchicago.edu)
Using two-dimensional gas chromatography coupled to time-of-flight mass spectrometry, they measured a total 851 metabolites, 43 of which differed significantly in expression patterns between TBI cases and controls. (medscape.com)

Further characterization was conducted with pseudo MS(3)experiments of the intact conjugates and by comparison of resulting product ion mass spectra with reference material. (uni-koeln.de)
The portable size ZipChip Interface directly mounts onto select models of Thermo Scientific mass spectrometers, and creates a seamless CE/ESI-MS workflow that offers fast CE separation, nano-spray level sensitivity, and HRAM spectrometry for the characterization of intact proteins, antibody drug conjugates (ADCs), antibody subunits, peptides, and metabolites. (thermofisher.com)
An Easy nL nano liquid chromatographic system is coupled to the mass spectrometer through the nanoelectrospray source for protein characterization. (boisestate.edu)
Protein identification, metabolite characterization, and general chemistry LC/MSn. (boisestate.edu)

HPLC-MS-based lipidomics was utilized to identify and quantify the endogenously altered lipid metabolites when hepatocytes were exposed to OA administration. (nofima.com)
However, the role of specific lipid metabolites on colorectal cancer risk is unclear. (who.int)
In a case-control study nested within the European Prospective Investigation into Cancer and Nutrition (EPIC), we examined associations between pre-diagnostic circulating concentrations of 97 lipid metabolites (acylcarnitines, glycerophospholipids and sphingolipids) and colorectal cancer risk. (who.int)
Multivariable conditional logistic regression was used to estimate odds ratios (ORs) and 95% confidence intervals (CIs) for associations between concentrations of individual lipid metabolites and metabolite patterns with colorectal cancer risk. (who.int)

In order to probe for the presence of additional trenbolone metabolites and to re-investigate the metabolism, an elimination study was conducted. (uni-koeln.de)
The importance of this procedure was demonstrated by putative identification of 690 metabolites in extracts of the protozoan parasite Trypanosoma brucei, thus allowing identified metabolites to be mapped onto an organism-wide metabolic network, providing opportunities for future studies of cellular metabolism from a global systems biology perspective. (manchester.ac.uk)
She specialises in understanding regulation of glucocorticoid action in metabolic and inflammatory diseases and has developed innovative tools to study steroid metabolism and flux in vivo, most recently by mass spectrometry imaging. (ed.ac.uk)
Lastly I apply mass spectrometry to enable tracking of steroid metabolism, using stable-isotope tracers and also advanced methods of MS detection e.g. imaging. (ed.ac.uk)
In addition to his research interests in chemical biology and microbiology of secondary metabolism in various organisms, Dr. Beld is deploying mass spectrometry in the Center for Advanced Microbial Processing. (drexel.edu)
Many other exciting projects in the field of secondary metabolism, pathogenic bacteria and mass spectrometry are being pursued. (drexel.edu)
Conclusion: Our data suggest a graded derangement of the circulating metabolite profile from lean to obese to MetS, in particular for metabolites involved in amino acid synthesis/metabolism and sphingolipid metabolism. (lu.se)
Researchers used mass spectrometry to analyze these for more than 600 byproducts of metabolism called metabolites. (msdmanuals.com)

Nerve agent metabolites, i.e., isopropyl methylphosphonic acid (IMPA) and pinacolyl methylphosphonic acid (PMPA), were derivatized by reacting them with 2,3,4,5,6-pentafluorobenzyl bromide (PFBBr) and were determined by mass spectrometry using an ultraviolet femtosecond laser emitting at 267 and 200 nm as the ionization source. (elsevierpure.com)
IMPA-PFB and PMPA-PFB, contain a large side-chain, and molecular ions are very weak or absent in electron ionization mass spectrometry. (elsevierpure.com)
The use of ultraviolet femtosecond laser ionization mass spectrometry, however, resulted in the formation of a molecular ion, even for compounds such as these that contain a highly bulky functional group. (elsevierpure.com)
Here, we show that, even using electrospray ionization, a soft ionization method, in-source fragmentation generates unwanted byproducts of identical mass to common metabolites. (princeton.edu)
Most common ionization technologies in the field of MSI are DESI imaging, MALDI imaging, secondary ion mass spectrometry imaging (SIMS imaging) and Nanoscale SIMS (NanoSIMS). (wikipedia.org)
This technique is performed using a focused ionization beam to analyze a specific region of the sample by generating a mass spectrum. (wikipedia.org)
Some of the criteria for choosing the ionization method are the sample preparation requirement and the parameters of the measurement, as resolution, mass range and sensitivity. (wikipedia.org)
Electrospray ionization tandem mass spectrometry (ESI-MS/MS) analysis of urinary metabolites of 1,1,2,2- tetrachloroethylene using an automated SPE sample preparation system. (cdc.gov)
Integrate capillary electrophoresis (CE) and electrospray ionization (ESI) into a single microfluidic device to rapidly prepare, separate, and electrospray biological samples directly into your Thermo Scientific mass spectrometer . (thermofisher.com)
In this webinar, the benefits of integrating cutting-edge microfluidic technology with capillary electrophoresis (CE) and electrospray ionization (ESI) for enhanced mass spectrometry (MS) analysis is presented. (thermofisher.com)
LC-MS) to achieve a conspicuous separation of metabolites before ionization. (frontiersin.org)
Their ionization efficiencies for mass spectrometry (MS) detection are rather poor, whereas their retention and separation on reverse phase liquid chromatography (RPLC) are often unsatisfying. (unboundmedicine.com)
Herein, we assembled plasmonic Janus hybrids for laser desorption/ionization mass spectrometry (LDI MS) detection of small metabolites. (rsc.org)

Gas chromatography MS is ideal for screening engineered bacterial strains for small metabolites like terpenes, fatty acids or small polyketides. (drexel.edu)

The concentrate is analyzed using liquid chromatography combined with tandem mass spectrometric (LC/MS/MS) determination. (cdc.gov)
Methods for the determination of MDMA and its metabolites, 4-hydroxy-3-methoxymethamphetamine (HMMA), 3,4-dihydroxy-methamphetamine (HHMA), 3,4-methylenedioxyamphetamine (MDA), 4-hydroxy-3-methoxyamphetamine (HMA), and 3,4-dihydroxyamphetamine (HHA), in biological fluids were established. (erowid.org)
A new method of the determination polycyclic aromatic hydrocarbons (PAHs) in water samples was developed by continuous-flow microextraction (CFME) coupled with gas chromatography-mass spectrometry (GC-MS). In this experiment, 15 mL sample solution with no salt-added was flowed at the rate of 1.0 mL min(-1) through 3 microL benzene as extraction solvent. (who.int)

The microbial community is also a natural source of metabolites and has the potential to be used to create antimicrobial and anti-biofilm agents. (frontiersin.org)
Adaptive laboratory evolution of microbial co-cultures for improved metabolite secretion. (embl.org)
Facilitating imaging mass spectrometry of microbial specialized metabolites with METASPACE. (embl.org)
Moreover, the MMKD significantly affected gut microbial populations, including mycobiome and stool metabolites. (news-medical.net)
Here, we show that antibiotic-induced reduction of gut microbial populations leads to an enrichment of nutrients and depletion of inhibitory metabolites, which enhances CRE growth. (imperial.ac.uk)
this is accompanied by depletion of microbial metabolites and enrichment of nutrients. (imperial.ac.uk)
Furthermore, certain microbial metabolites (depleted upon antibiotic treatment) inhibit CRE growth. (imperial.ac.uk)
Our results show that killing gut commensals with antibiotics facilitates CRE colonisation by enriching nutrients and depleting inhibitory microbial metabolites. (imperial.ac.uk)

Liquid chromatography-mass spectrometry (LC-MS) technology allows for rapid quantitation of cellular metabolites, with metabolites identified by mass spectrometry and chromatographic retention time. (princeton.edu)
Accordingly, chromatographic separation is required for accurate quantitation of many common cellular metabolites. (princeton.edu)
Xu, YF, Lu, W & Rabinowitz, JD 2015, ' Avoiding misannotation of in-source fragmentation products as cellular metabolites in liquid chromatography-mass spectrometry-based metabolomics ', Analytical Chemistry , vol. 87, no. 4, pp. 2273-2281. (princeton.edu)

In the analysis of human urine sample by liquid chromatography MS/MS, four spectral matches were found from the 9 microsome-produced metabolite standards. (nih.gov)
The detection of trenbolone doping in routine sports drug testing programs is complex as methods utilizing gas chromatography/mass spectrometry are complicated by unspecific derivatization products and artifacts, and liquid chromatography/mass spectrometry-based assays have shown to allow for comparably high limits-of-detection only. (uni-koeln.de)
The liquid chromatography - mass spectrometry metabolomics of bacterial metabolites produced under stress conditions is also in my interest. (researchgate.net)
Coupled with sensitive liquid chromatography we have a pipeline from genome to secondary metabolite. (drexel.edu)
Liquid chromatography mass spectrometry setups in the New College Building include (from left to right) a Waters Acquity UPLC-Synapt G2Si QTOF, Thermo Eksigent 2d-nano-lc-LTQ linear ion trap, Waters Alliance HPLC-QDA single quad and Sciex Perkin Elmer-triple-quad (Waters Alliance-ZQD single quad not shown). (drexel.edu)
To generate metabolomic profiles of plasma and serum samples, the researchers used gas chromatography-mass spectrometry and liquid chromatography-tandem mass spectrometry to identify 477 metabolites. (medscape.com)

We evaluated yeast intracellular metabolite extracts and found more than 20 cases of in-source fragments that mimic common metabolites. (princeton.edu)

Lastly, the microfluidic CZE-MS methods were further applied for both primary and secondary metabolite profiling in a DMPK assay. (uky.edu)
Of all the drugs used in a hospital to treat patients, about 75% are secondary metabolite or secondary metabolite derived molecules. (drexel.edu)

Priority pesticides (non-persistent pesticide metabolites) were measured using two mass spectrometric methods. (cdc.gov)
This method is used for determining concentrations of select pesticide metabolites in the human population. (cdc.gov)

The number of previously reported metabolites in human urine is limited, and most analytical methods rely on targeting epitrenbolone, trenbolone glucuronide, and epitrenbolone glucuronide. (uni-koeln.de)
The procedure involves enzymatic hydrolysis of urine, extraction, derivatization, and column cleanup, followed by analysis of the concentrate using capillary gas chromatography combined with tandem mass spectrometry (GC-MS/MS). This procedure uses isotope dilution with 13 C-labeled internal standards for all analytes. (cdc.gov)
Urine samples from rats exposed to 100, 500, or 1000 mg/kg PERC contained detectable amounts of PERC metabolites in urine over a 48-hr period. (cdc.gov)

The environmental mass spectrometry laboratory has over 30 years of experience in the analysis of soil and sediments, and gas samples. (ncl.ac.uk)
The environmental mass spectrometry laboratory can perform both routine and bespoke GC and GC-MS analysis with integrated liquid and pyrolysis autosamplers, and gas sampling valves. (ncl.ac.uk)
Additionally, methods for the analysis of polyphenolic plant-derived secondary metabolites, such as cannabinoids, were also developed and evaluated. (uky.edu)
A simple, reliable, and accurate method was developed for quantitative assessment of metabolite coverage in preclinical safety species by mixing equal volumes of human plasma with blank plasma of animal species and vice versa followed by an analysis using high-resolution full-scan accurate mass spectrometry. (nih.gov)
In addition, both qualitative and quantitative data were obtained from a single LC-MS analysis on all metabolites and, therefore, the coverage of any metabolite of interest can be obtained. (nih.gov)
Today, mass spectrometry is a well-established detection method that offers a multitude of benefits, such as selectivity, sensitivity, and multi-sample analysis. (sigmaaldrich.com)
A complete workflow for the intact and middle-up mass analysis of reduced and non-reduced monoclonal antibodies based on SEC-MS with sample preparation by protein-A affinity clean-up. (sigmaaldrich.com)
This facility provides services for analysis of metabolites and lipids. (embl.org)
Rapid automated annotation and analysis of N-glycan mass spectrometry imaging data sets using NGlycDB in METASPACE. (embl.org)
Subsampling is needed to ensure an adequate quantity of sample for analysis and the mass spectrometry analytical methods. (cdc.gov)
They extracted deoxyribonucleic acid (DNA) for metagenomic sequencing and metabolites for untargeted tandem mass spectrometry (MS/MS) analysis. (news-medical.net)
The advantage of this method lies in the possibility to get a global and comprehensive analysis of the sample without predetermining which metabolites should be measured. (ufz.de)
Differential metabolites were subjected to Metabolite Set Enrichment Analysis (MSEA) and genes corresponding to proteins having interactions with metabolites were further annotated with Gene Ontology (GO). (bvsalud.org)

Coupling proteomics and metabolomics for the unsupervised identification of protein-metabolite interactions in Chaetomium thermophilum. (embl.org)
We established the ProMetheus platform for mass spectrometry-based (meta)proteomics and metabolomics in 2008. (ufz.de)

Metabolites are often present at a broad range of concentrations and possess diverse physical properties complicating this task. (oregonstate.edu)
More, this technique is widely regarded as one of the most sensitive forms of mass spectrometry as it can detect elements in concentrations as small as 1012-1016 atoms per cubic centimeter. (wikipedia.org)
Due to the sensitivity of mass spectrometry, it is widely used to measure very low molecular weights at extremely low concentrations, below nanograms per milliliter (ng/mL). (sigmaaldrich.com)
Beginning with NHANES 2005-2006, CDC used a weighted pooled-sample design to measure serum concentrations of dioxins, furans, polychlorinated biphenyls (PCBs), organochlorine pesticides and metabolites, and brominated flame retardants (polybrominated diphenyl ethers and polybrominated biphenyl [PBB] 153). (cdc.gov)

In ProMetheus , we have established untargeted and targeted measurement of proteins and metabolites, metaproteomics, and novel data evaluation workflows for in-house and external use. (ufz.de)

Main metabolites were attributed to trenbolone-diol and potential trenbolone-diketone derivatives excreted as glucuronic acid and sulfo-conjugated analytes with detection windows of 5, respectively 6 days. (uni-koeln.de)
The common method used is gas chromatography (GC) coupled with electron capture detection (ECD) followed by identification using GC/mass spectrometry (MS). Since evidence indicates that heptachlor is metabolized to heptachlor epoxide in mammals, exposure to heptachlor is usually measured by determining levels of heptachlor epoxide in biological media. (cdc.gov)
Moreover, plasmonic nanoshells enabled direct metabolite detection with silver adduction in bio-mixtures, due to the selective LDI process. (rsc.org)

Furthermore, we demonstrated the tunable surface chemistry of Janus hybrids for the enrichment and identification of metabolites at low concentration (4 pM). (rsc.org)

At its most ambitious, untargeted metabolomics aims to characterize and quantify all of the metabolites in a given system. (oregonstate.edu)
And, when calibration standards are used, mass spectrometry is well suited to absolutely quantify the targeted analytes. (ufz.de)

The metabolites produced by HLM can be tentatively identified based on the associated substrate structure, known metabolic processes, tandem mass spectrometry (MS/MS) fragmentation patterns and, if necessary, accurate mass measurements. (nih.gov)
Simultaneous, untargeted metabolic profiling of polar and nonpolar metabolites by LC-Q-TOF mass spectrometry. (oregonstate.edu)
This protocol describes the untargeted, metabolic profiling of polar and nonpolar metabolites with a single extraction and using a single analytical platform. (oregonstate.edu)

Trichloroacetic acid (TCA, CAS 76-03-9) and dichloroacetic acid (DCA, CAS 79-43-6) are reported urinary metabolites of PERC oxidation wherease the glutathione conjugate, S-(1,2,2-trichlorovinyl) -L-glutathione (TCVG) is further cleaved to S-(1,2,2-trichlorovinyl)-L-cysteine (TCVC). (cdc.gov)
Conjugated HMMA and HHMA are the main urinary metabolites of MDMA. (erowid.org)
Mono- n -octyl phthalate (MOP), a phthalate urinary metabolite, was not detected above the 95th percentile for more than three survey cycles, so it was no longer reported after 2010. (cdc.gov)

Recently, with the development of rapid scanning high-resolution high accuracy mass spectrometers and the desire for high throughput screening, minimal or no chromatographic separation has become increasingly popular. (princeton.edu)
When analyzing complex cellular extracts, however, the lack of chromatographic separation could potentially result in misannotation of structurally related metabolites. (princeton.edu)

Phenytoin-initiated micronucleus formation was increased about 4-fold in both +/ j (P = .006) and j/j (P = .009) UGT-deficient cells vs. +/+ UGT-normal cells, providing the first evidence that the bioactivation and oxidative toxicity of phenytoin itself may be avoided by direct N -glucuronidation, which was confirmed by tandem mass spectrometry. (aspetjournals.org)
Public LC-Orbitrap tandem mass spectral library for metabolite identification. (embl.org)
PERC metabolites (>90% recovery) were analyzed using a ThermoQuest Finnegan LCQ tandem mass spectrometer. (cdc.gov)
Mass spectrometry, specifically tandem mass spectrometry (MS/MS) is the method of choice to mine complex samples for new natural products. (drexel.edu)
The introduction of tandem mass spectrometry made it possible to screen for many conditions at the same time using a single test, and led to a huge expansion in the number of conditions that could be included, with some U.S. states now screening for over 50 conditions. (cdc.gov)
Title : Evaluation of an Isotope Dilution HPLC Tandem Mass Spectrometry Candidate Reference Measurement Procedure for Total 17-β Estradiol in Human Serum Personal Author(s) : Botelho, Julianne Cook;Ribera, Ashley;Cooper, Hans C.;Vesper, Hubert W. (cdc.gov)

The Netzsch Jupiter STA 449C is a thermogravimetric analyser interfaced to an Aeolos QMS 403 quadrupole mass spectrometer. (ncl.ac.uk)

The development of sensitive, high-throughput analytical screening methods for plant derived metabolites is crucial for natural pharmaceutical product discovery and plant metabolomic profiling. (uky.edu)
This procedure appears to offer significant advantages over typical extraction and derivatization methods for the same compounds by GC-MS. Thus, PERC internal dose for various exposures can be rapidly quantified using metabolites in a single assay using an automated sample preparation system. (cdc.gov)
Multiplexed Profiling and Data Processing Methods to Identify Temperature-Regulated Primary Metabolites Using Gas Chromatography Coupled to Mass Spectrometry. (mpg.de)

An alkaloid screening assay was constructed to analyze transgenic mutant plant extracts for novel metabolites. (uky.edu)

The biomarker signature includes 9 metabolites as well as the carbohydrate antigen 19-9 (CA19-9), which is currently the only blood-based biomarker in clinical use for pancreatic cancer. (medscape.com)

Commonly used spectrometric techniques are nuclear magnetic resonance (NMR) and mass spectrometry (MS). Each method has its own pros and cons, while MS prevails in sensitivity and selectivity ( Emwas, 2015 ). (frontiersin.org)
Similar metabolites and pathways were also related to insulin sensitivity (Matsuda index) in a separate study (POEM, n=501). (lu.se)

Once identified, these metabolites can be used as references for identification of the same compounds in complex biological samples. (nih.gov)
We use gas chromatography (GC) to resolve complex mixtures of gases, and volatile and semi-volatile compounds, coupled with mass spectrometry (GC-MS) and other detectors such as flame ionisation (FID) and electron capture (ECD). (ncl.ac.uk)
Secondary metabolites are organic compounds produced by an organism for reasons other than growth and development. (uky.edu)
Mass spectrometry (MS) is an analytical technique that uses mass-to-charge ( m/z ) ratio to identify compounds in a sample. (sigmaaldrich.com)
Of these metabolites, 10 compounds made up the final panel. (medscape.com)

The laboratory also has LC-MS systems and a thermogravimetric analyser interfaced to a mass spectrometer (TGA-MS). (ncl.ac.uk)
A mass spectrometer ionizes the sample into gaseous ions and then identifies the ions by their mass-to-charge ratios and relative abundances. (sigmaaldrich.com)
A mass spectrometer works by converting individual molecules into ions and then analyzing the relative abundance of the generated ions. (sigmaaldrich.com)
In the ion chamber of a mass spectrometer, each individual molecule is ionized to form a molecular ion, having one electron less than the parent molecule. (sigmaaldrich.com)
From one complex sample, a mass spectrometer generates many ions. (sigmaaldrich.com)
Analytes are electrosprayed directly into the mass spectrometer through the ESI emitter on the corner of the microfluidic chip. (thermofisher.com)
Bruker Q-TOF Mass Spectrometer is located in the mass spectrometer is located in the BRC, room 215 in the Mathematics Building on the campus of Boise State University. (boisestate.edu)
This mass spectrometer is coupled with a Dionex Ultimate 3000 HPLC system and an innovative Captive electrospray source. (boisestate.edu)
Bruker HCT Ion Trap Mass Spectrometer from Bruker Daltonics is located in the Analytical Lab, room 363 in the Science building on the Boise State University campus. (boisestate.edu)
The HCT is a robust and easy to use mass spectrometer. (boisestate.edu)
The Thermo Polaris Q GC/MSn Benchtop Ion Trap Mass Spectrometer complete with Thermo Trace Gas Chromatograph, external vacuum pump, and XCalibur PC workstation. (boisestate.edu)
HTX Imaging's patented TM-Sprayer™ Tissue MALDI Sample Preparation System is designed to provide the highest quality matrix deposition on the two-dimensional biological samples, and can be integrated with any MALDI Mass Spectrometer platform. (boisestate.edu)
The state of the art equipment allows us to select the best solution in respect to the type of mass spectrometer. (ufz.de)
1) Waters Acquity HPLC system coupled to an AB Sciex QTRAP 5500 mass spectrometer. (ufz.de)

Pesticide residues and their metabolites in human tissues and fluids can be indicative of pesticide exposure and the total body burden of these pesticides. (cdc.gov)
We measure metabolites of indoor as well as outdoor pollutants, ranging from phthalates to pesticides. (lu.se)

The ability to couple mass spectrometry to other separation techniques such as capillary electrophoresis, GC, and HPLC makes it a versatile analytical tool for the simultaneous separation and identification of analytes. (sigmaaldrich.com)
A rapid method for the separation of vitamin D and its metabolites by ultra-high performance supercritical fluid chromatography-mass spectrometry. (lu.se)

They also found evidence of significant upregulation of several metabolites in the cerebrospinal fluid and brain microdialysate samples of newly arrived patients with severe TBI, suggesting that blood-brain barrier had been disrupted. (medscape.com)

The metabolites of note included small fatty acids such as decanoic and octanoic acid, amino acids such as serine and 1H-indole-3-acetic acid, and sugar derivatives such as glycerol. (medscape.com)

Secondary ion mass spectrometry (SIMS) is used to analyze solid surfaces and thin films by sputtering the surface with a focused primary ion beam and collecting and analyzing ejected secondary ions. (wikipedia.org)
The ions are then accelerated in an electromagnetic field and separated based on their mass-to-charge ( m/z ) ratios. (sigmaaldrich.com)
The instrument's detector records the ions in proportion to their relative abundance and generates a mass spectrum of the molecule. (sigmaaldrich.com)

In this study, we investigated the in vitro inhibitory effects of the individual major cannabinoids and metabolites ∆9-tetrahydrocannabinol (THC), cannabidiol (CBD), cannabinol (CBN), 11-nor-THC-carboxylic acid, and 11-hydroxy-THC on CES1 activity. (aspetjournals.org)
With increasing cannabis use, the potential for cannabis or one or more of its physically active constituents (cannabinoids) and their major metabolites interacting with other therapeutic agents also increases. (aspetjournals.org)

However, difficulties associated with the identification of detected metabolites currently limit its application. (manchester.ac.uk)
The predicted retention times improved metabolite identification by removing 40% of the false identifications that occurred with identification by accurate mass alone. (manchester.ac.uk)

The sample preparation strategy consisted of solid-phase extractions, liquid-liquid extractions, metabolite de-conjugation, HPLC fractionation, and derivatization. (uni-koeln.de)

A quantitative structure retention relationship (QSRR) model, incorporating six physicochemical variables in a multiple-linear regression based on 120 authentic standard metabolites, shows good predictive ability for retention times of a range of metabolites (cross-validated R 2 = 0.82 and mean squared error = 0.14). (manchester.ac.uk)

Individuals from 3 population-based samples (EpiHealth, n=2342, SCAPIS-Uppsala, n=4985, and SCAPIS-Malmö, n=3978) were divided into groups according to their body mass index (BMI) and presence/absence of MetS (National Cholesterol Education Program [NCEP]/consensus criteria). (lu.se)
Samples taken the day after the injury continued to show marked differences between patients with severe TBI and controls, metabolite profiles similar to those seen in the samples obtained at patient presentation. (medscape.com)

My research interests are in steroid biochemistry and applied mass spectrometry. (ed.ac.uk)

In the past saliva was mainly considered as a digestive fluid that plays an important role in the oral processing of foods, but recent studies have shown that its metabolite composition is as complex as that of serum, and that it offers a promising pain-free alternative to invasive sampling. (mdpi.com)
A study comparing serum metabolite levels in adults with acute TBI with those of patients with acute orthopedic trauma but no acute or previous brain trauma showed that expression patterns of 43 metabolites significantly differed between TBI cases and controls, and that the differences were most pronounced among patients with severe TBI, said Jussi Posti, MD, PhD, a neurosurgeon at Turku University Hospital in Finland. (medscape.com)
The investigators also created a statistical model to see whether serum metabolites could predict patient outcomes, determined by a Glasgow Outcomes Scale (extended) score of 4 (upper severe disability) vs greater than 4. (medscape.com)

Metabolites;12(12)2022 Dec 19. (bvsalud.org)

We measure the nutrient utilisation abilities, nutrient preferences, and metabolite inhibition susceptibilities of several CRE strains. (imperial.ac.uk)

Quantification of Stable Isotope Label in Metabolites via Mass Spectrometry. (mpg.de)

A special issue of Metabolites (ISSN 2218-1989). (mdpi.com)

The structures of the isolated metabolites were determined by high field NMR spectroscopy and HR mass spectrometry. (lu.se)

Anatomy13

Organisms12

Diseases1

Chemicals and Drugs85

Analytical, Diagnostic and Therapeutic Techniques and Equipment74

Phenomena and Processes56

Disciplines and Occupations9

Anthropology, Education, Sociology and Social Phenomena3

Technology, Industry, Agriculture2

Information Science8

Health Care13

Human metabolites6

Biomarkers6

Endogenous6

Identify metabolites3

High Resolution Mass Spectrometry2

Time-of-flight ma2

Characterization4

Lipid metabolites4

Metabolism8

Ionization13

Small metabolites1

Determination3

Microbial8

Cellular metabolites3

Liquid Chromatography6

Common metabolites1

Secondary metabolite2

Pesticide metabolites2

Urine3

Analysis13

Proteomics and metabolomics2

Concentrations4

Proteins1

Detection3

Enrichment1

Quantify2

Metabolic3

Urinary3

Chromatographic2

Tandem mass6

Quadrupole mass1

Methods3

Extracts1

Biomarker1

Sensitivity2

Compounds5

Spectrometer14

Pesticides2

Separation2

Cerebrospinal1

Fatty acids1

Ions3

Cannabinoids2

Identification2

Derivatization1

Quantitative1

Samples2

Biochemistry1

Serum3

20221

Inhibition1

Quantification1

ISSN1

Spectroscopy1