• To identify genes that depend upon, or are involved in, this coordinate regulation we screened for heterozygous chromosome 3 deficiencies that impair development of flies bearing reduced H3.2 and H3.3 gene copy number. (bvsalud.org)
  • We find that during normal lifespan of CMs gene regulation is mainly orchestrated by dynamic mCpG and canonical histone marks at distal regulatory and genic regions. (nature.com)
  • The goal of my laboratory is to understand how blood vessels grow, differentiate and regress through studying the transcriptional regulation of vascular genes. (ox.ac.uk)
  • To study vessel regulation, my laboratory primarily focuses on the identification, characterisation and delineation of enhancers ( cis -regulatory elements) directing gene expression within the vasculature. (ox.ac.uk)
  • Given the significance of myogenin regulation in adult muscle, the Akt/MSY3/myogenin regulatory circuit is a potential therapeutic target to counteract muscle degenerative disease. (biomedcentral.com)
  • Thus, these studies identify the HRC enhancer as the first MEF2-dependent, CArG-independent transcriptional target in smooth muscle and represent the first analysis of the transcriptional regulation of an SR gene in vivo. (ox.ac.uk)
  • Further exploration of PDF neuron morphological changes and the role of Mef2 might be a useful platform to dissect the interface between the contributions of circadian and homeostatic processes to sleep-wake regulation. (alkpathway.com)
  • Regulation of gene expression plays a central role in embryonic development. (biomedcentral.com)
  • The overall results provide a systematic view of gene regulation in the human, which will be refined as additional mammalian genomes become available. (gsea-msigdb.org)
  • Transcription factor GATA4-targeted compounds that have previously shown in vivo efficacy in cardiac injury models were tested for stage-specific activation of atrial and ventricular reporter genes in differentiating pluripotent stem cells using a dual reporter assay. (biomedcentral.com)
  • Epigenetic mechanisms and transcription factor networks essential for differentiation of cardiac myocytes have been uncovered. (nature.com)
  • Enhancers are densely clustered groups of transcription factor binding motifs and are the principal regulators of spatio-temporal patterns of gene transcription. (ox.ac.uk)
  • Proteasome activity is also affected by neuronal activity, via increased expression of UBE3A through transcription factor MEF2, which leads to the internalization of AMPA-R. Variations in the Calcium-channel CACNA1C are also associated with ASD and [https://en.wikipedia.org/wiki/Timothy_syndrome Timothy Syndrome]. (wikipathways.org)
  • Here, employing an inducible B cell expression system, we have determined that M2 activates the NFAT pathway in a Src kinase-dependent manner - leading to induction of the plasma cell-associated transcription factor, Interferon Regulatory Factor-4 (IRF4). (prelekara.sk)
  • The dynamic cytoskeletal localization of Csl and its dominant effects on cell shape and behavior and transcription factor activity suggest that Csl plays a role in the regulatory network through which muscle cells coordinate their structural and functional states during growth, adaptation, and repair. (ox.ac.uk)
  • We identified two regions of chromosome 3 that conferred this phenotype, one of which contains the Polycomb gene, which is necessary for establishing domains of facultative chromatin that repress master regulator genes during development. (bvsalud.org)
  • In contrast, pathological gene expression in terminal heart failure is accompanied by changes in active histone marks without major alterations in CpG methylation and repressive chromatin marks. (nature.com)
  • The major event of MBT was the activation of a large group of histones and other genes that modify chromatin structure preceding massive gene expression changes. (biomedcentral.com)
  • Fusion in humans is aided by a set of metalloproteinases coded for by the ADAM12 gene, and a variety of other proteins. (wikipedia.org)
  • The Arabidopsis genome contains an estimated 340,000 potential MADS binding sites ( de Folter and Angenent, 2006 ), leading to the intriguing question: What is the biological significance of the large number of potential MADS binding sites, and how many target genes do MADS proteins really regulate? (frontiersin.org)
  • Based on these studies, it appears that MADS proteins may directly regulate thousands of target genes (Figure 1 A), and may serve to integrate different biological processes. (frontiersin.org)
  • (A) Network representation (Cytoscape) of the reported target genes (represented by blue lines) of the four MADS proteins (yellow circles). (frontiersin.org)
  • The four MADS proteins share 70 target genes. (frontiersin.org)
  • (C) Wire diagram (BioTapestry) showing the complex regulatory network between the four MADS proteins themselves. (frontiersin.org)
  • Chemically induced gene expression changes were characterized by qRT-PCR, global run-on sequencing (GRO-seq) and immunoblotting, and the network of cooperative proteins of GATA4 and NKX2-5 were further explored by the examination of the GATA4 and NKX2-5 interactome by BioID. (biomedcentral.com)
  • Analysis of the proteins that activate and repress different enhancers is combined with genetic studies to accurately position these factors within complex signalling networks. (ox.ac.uk)
  • Within this conserved region is a consensus site for myocyte enhancer factor 2 (MEF2) proteins that we show is bound efficiently by MEF2 and is required for transgene expression in all three muscle lineages in vivo. (ox.ac.uk)
  • Explore our solutions for multiple applications - vaccines, monoclonal antibodies, recombinant proteins, cell or gene therapy. (vwr.com)
  • knockdown than Mef2 overexpression may indicate that misexpression of other Mef2 target genes within PDF neurons synergizes with the constant defasciculation to negatively impact behavioral rhythmicity. (alkpathway.com)
  • These mice express tamoxifen-induced Cre recombinase (Mer-cre-Mer) that enzymatically removes a stop codon flanked by lox sequences (floxed) on a reporter gene such as green fluorescent protein or yellow fluorescent protein, thus resulting in traceable fluorescence on the cells of interest. (musculoskeletalkey.com)
  • In this study, we identified a transcriptional enhancer from the HRC gene that is sufficient to direct the expression of lacZ in the expression pattern of endogenous HRC in transgenic mice. (ox.ac.uk)
  • If these mice were engineered to overexpress MEF2 at a higher risk for these individuals in a mouse model of neurodegeneration, we saw that it regulates. (hamiltonpr.net)
  • Collectively, these results indicate the potential for therapeutic alteration of cell fate decisions and pathological gene regulatory networks by GATA4-targeted compounds modulating chamber-specific transcriptional programs in multipotent cardiac progenitor cells and cardiomyocytes. (biomedcentral.com)
  • In contrast to previous findings in heart tissue, expression of the pathological gene program in heart failure was not accompanied by changes in the CM DNA methylome but by active histone marks. (nature.com)
  • HRC is a direct transcriptional target of MEF2 during cardiac, skeletal, and arterial smooth muscle development in vivo. (ox.ac.uk)
  • Our research goals are to define the transcriptional networks and downstream mechanisms that link the molecular clock with proper skeletal muscle function and phenotype. (ufl.edu)
  • Multi-omic identification of key transcriptional regulatory programs during endurance exercise training. (ufl.edu)
  • The nature of imperfect binding specificity means that a single miRNA can regulate a large number of mRNA targets involved in complex cellular processes, thereby tightly controlling genetic networks during development and in response to stress ( Pocock, 2011 ). (elifesciences.org)
  • Our results suggest that previous estimates of the number of human miRNA genes were low, and that miRNAs regulate at least 20% of human genes. (gsea-msigdb.org)
  • In this line, reporter gene assays with combinatorial treatment of 3i-1000 and the BET bromodomain inhibitor (+)-JQ1 demonstrated the cooperative role of GATA4 and BRD4 in the modulation of chamber-specific cardiac gene expression. (biomedcentral.com)
  • We find that prenatal development and postnatal maturation are characterized by a cooperation of active CpG methylation and histone marks at cis -regulatory and genic regions to shape the cardiac myocyte transcriptome. (nature.com)
  • Notably, cis -regulatory regions in cardiac myocytes are significantly enriched for cardiovascular disease-associated variants. (nature.com)
  • 2011. Gene Coexpression Network Topology of Cardiac Development, Hypertrophy, and FailureClinical Perspective . (umd.edu)
  • The HRC gene encodes the histidine-rich calcium-binding protein, which is found in the lumen of the junctional sarcoplasmic reticulum (SR) of cardiac and skeletal muscle and within calciosomes of arterial smooth muscle. (ox.ac.uk)
  • In any case, even the knockdown of Mef2 has no behavioral phenotype despite the lack of circadian plasticity and constant fasciculation. (alkpathway.com)
  • These findings constitute the first reported phenotype associated with SNPs in the EF-G2mt gene and implicate the human EF-G2mt gene as a pharmacogenetic candidate gene for statin toxicity in humans. (prolekarniky.cz)
  • Basic helix-loop-helix (bHLH) myogenic regulatory factors coordinate the correct function and organization of many skeletal muscle functions. (biomedcentral.com)
  • Using genetic mouse models, we found that mutations of two different molecular clock genes, Clock and Bmal1, dramatically disrupt skeletal muscle structure and function. (ufl.edu)
  • The vascular system is a highly branched network of endothelial cell-lined tubes that transports blood, metabolites and waste products throughout the body. (ox.ac.uk)
  • Special Lecture on Neuro antidepressant drugs detailed both published and newly released findings of the patients evaluated by neurologists and represented in ENERGY have a history of vascular problems such as those in the human study, MEF2 was more active in the. (hamiltonpr.net)
  • To understand the development of adiposity, it is crucial to identify the genes which expression is associated with adipogenic differentiation. (biomedcentral.com)
  • Proliferation cluster comprised 1028 genes up-regulated from days 3 to 8 of culture meanwhile the differentiation cluster was characterized by 2140 induced genes from days 15 to 21. (biomedcentral.com)
  • On the other hand, the terminal differentiation phase was enriched with genes involved in energy production, lipid and carbohydrate metabolism. (biomedcentral.com)
  • Overall, our study demonstrates the coordinated expression of functionally related genes during proliferation and differentiation of rainbow trout adipocyte cells. (biomedcentral.com)
  • Here, we demonstrate that variant histone H3.3 is essential for Drosophila development only when canonical histone gene copy number is reduced, suggesting that coordination between canonical H3.2 and variant H3.3 expression is necessary to provide sufficient H3 protein for normal genome function. (bvsalud.org)
  • Moreover, heterozygous Polycomb mutations result in de-repression of the Polycomb target gene Ubx and cause ectopic sex combs when either canonical or variant H3 gene copy number is reduced. (bvsalud.org)
  • Some loss-of-function mutations of the TWIST1 gene have been shown to cause an autosomal dominant craniosynostosis, known as the Saethre-Chotzen syndrome (SCS). (biomedcentral.com)
  • Multiple mutations affecting embryonic development have been induced by chemical and insertional mutagenesis resulting in the identification of genes with important roles in development in Drosophila [ 23 - 25 ]. (biomedcentral.com)
  • The number of MADS genes varies greatly among taxa, with yeast (4) and animals (5) having modest numbers. (frontiersin.org)
  • Based on yeast genetic studies, we identify the mitochondrial translation factor MEF2 as a mediator of atorvastatin toxicity. (prolekarniky.cz)
  • Using yeast as a model, conserved amino acid variants, which arise from non-synonymous single nucleotide polymorphisms (SNPs) in the EF-G2mt gene, were generated in the yeast MEF2 gene. (prolekarniky.cz)
  • Novel genes and their protein products that are expressed during the process are under active investigation in many laboratories. (wikipedia.org)
  • Proliferation was characterized by enrichment in genes involved in basic cellular and metabolic processes (transcription, ribosome biogenesis, translation and protein folding), cellular remodelling and autophagy. (biomedcentral.com)
  • In addition, due to their rapid and reversible regulatory capacity, miRNAs are prime candidate facilitators of responses to proteotoxic stress. (elifesciences.org)
  • Nearly one-half are associated with microRNAs (miRNAs), leading to the discovery of many new miRNA genes and their likely target genes. (gsea-msigdb.org)
  • Reporter gene assays were conducted to examine combinatorial effects of GATA-targeted compounds and bromodomain and extraterminal domain (BET) inhibition on chamber-specific gene expression. (biomedcentral.com)
  • Myogenic regulatory factors (MRFs): MyoD, Myf5, Myf6 and Myogenin. (wikipedia.org)
  • (B) Venn diagram depicting the numbers of shared target genes. (frontiersin.org)
  • ChIP-seq was performed using antibodies against SEP3 in wild type and agamous ( ag-1 ) inflorescence tissues, resulting in 4282 and 2828 peaks (binding events) respectively, corresponding to 3475 and 2424 putative target genes. (frontiersin.org)
  • In this study the authors were also interested in identifying target genes associated with perianth development. (frontiersin.org)
  • Here, we present a comparative analysis of the human, mouse, rat and dog genomes to create a systematic catalogue of common regulatory motifs in promoters and 3' untranslated regions (3' UTRs). (gsea-msigdb.org)
  • Genes, Brain and Behavior. (nebraska.edu)
  • Even after this paxil ends, new ones may arise in the development and progression of many neurodegenerative diseases and injuries of the other genes that MEF2 appears to cause brain damage. (hamiltonpr.net)
  • Mitochondrial translation, essential for synthesis of the electron transport chain complexes in the mitochondria, is governed by nuclear encoded genes. (prolekarniky.cz)
  • Several components of the electron transport chain are encoded in the mitochondrial genome, the translation of which is governed largely by nuclear encoded genes. (prolekarniky.cz)
  • Due to cellular heterogeneity in the heart, the activation of gene programs representing specific atrial and ventricular cardiomyocyte subtypes would be highly desirable. (biomedcentral.com)
  • More likely, however, the generation of memory-storing neural activity reflects a combination of cellular, synaptic, and network properties (Major and Tank, 2004). (alkpathway.com)
  • Enthesis development has historically been studied with lineage tracing of individual genes selected a priori, which does not allow for the determination of single-cell landscapes yielding mature cell types and tissues. (elifesciences.org)
  • Furthermore, the information generated will allow future investigations of specific genes involved in particular stages of fish adipogenesis. (biomedcentral.com)
  • Knowledge of the genetic networks controlling embryogenesis has been obtained principally by mutagenesis screens in model species. (biomedcentral.com)
  • Genes having at least one occurrence of the highly conserved motif M38 TGACCTTG in the regions spanning 4 kb centered on their transcription starting sites [-2kb, +2kb]. (gsea-msigdb.org)
  • Serum response factor (SRF) plays a central role during myogenesis, being required for the expression of striated alpha-actin genes. (wikipedia.org)
  • The human ortholog of MEF2 is the Elongation Factor Gene (EF-G) 2, which has previously been shown to play a specific role in mitochondrial ribosome recycling. (prolekarniky.cz)
  • Using small interfering RNA (siRNA) silencing of expression in human cell lines, we demonstrate that the EF-G2mt gene is required for cell growth on galactose medium, signifying an essential role for this gene in aerobic respiration. (prolekarniky.cz)
  • Regulatory pathways governing murine coronary vessel formation are dysregulated in the injured adult heart. (ox.ac.uk)
  • Myf5 is considered to be the earliest expressed regulatory factor gene in myogenesis. (wikipedia.org)
  • 2009. Gene Profiling of Human Adipose Tissue During Evoked Inflammation In Vivo . (umd.edu)
  • The first stage, involves cell cycle exit and the commencement of expression of certain genes. (wikipedia.org)
  • Numerous genes transcribed in oocytes are involved in multiple aspects of cell maintenance and protection, including metabolism, signal perception and transduction, RNA processing, cell cycle, defense against pathogens and DNA damage. (biomedcentral.com)
  • Our bioprocessing products are engineered to the highest quality standards and regulatory requirements. (vwr.com)
  • Maternal to zygotic transition (MZT) is the key event during embryogenesis marked by the switch of control from the maternal and possibly paternal transcripts to the newly synthesized embryonic gene products [ 9 - 11 ]. (biomedcentral.com)
  • The authors provided convincing evidence for the heterogeneity of postnatal enthesis growth and the molecular dynamics and signaling networks during enthesis formation. (elifesciences.org)
  • Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. (thermofisher.com)