Marker molecular weight. Medical search. Frequent questions

A molecular-weight size marker, also referred to as a protein ladder, DNA ladder, or RNA ladder, is a set of standards that are used to identify the approximate size of a molecule run on a gel during electrophoresis, using the principle that molecular weight is inversely proportional to migration rate through a gel matrix. (wikipedia.org)
As a result, a DNA "ladder" composed of DNA pieces of known molecular mass is created on the gel. (wikipedia.org)
Factors such as buffer, charge/voltage, and concentration of gel can affect the mobility and/or appearance of your marker/ladder/standard. (wikipedia.org)
G-Biosciences provide two prestained protein molecular weight markers (protein ladder, protein standard). (gbiosciences.com)
1 kb Plus DNA Ladder for Safe Stains is a pre-mixed, ready-to-load molecular weight marker containing one tracking dye. (neb.com)
1 kb Plus DNA Ladder for Safe Stains consists of proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as molecular weight standards for agarose gel electrophoresis. (neb.com)

A blue protein standard with 12 prestained proteins covering a wide range of molecular weights: 10-240kDa in Tris-Glycine buffer and 9-235kDa in Bis-Tris (MOPS) buffer or Bis-Tris (MES) buffer. (gbiosciences.com)
A ready-to-use three-color protein standard with 13 prestained proteins covering a wide range of molecular weights: 5-245kDa in Tris-Glycine buffer or 3.5-235kDa in Bis-Tris (MOPS) buffer or Bis-Tris (MES) buffer). (gbiosciences.com)
By identifying many sGAG-specific interacting proteins, our data provide a resource for upcoming studies aimed at molecular mechanisms and understanding of sGAG cellular effects. (degruyter.com)
These data indicate that olfactory receptor (OR) proteins are indeed present in the axonal processes and nerve terminals of olfactory sensory neurons, thus supporting the notion that ORs may participate in the molecular processes underlying the fasciculation and targeting of olfactory axons. (jneurosci.org)
MUC proteins have great significance as prognostic and diagnostic markers as well as a potential target for therapeutic interventions in most cancers of glandular epithelial origin. (amrita.edu)
These are high molecular weight glycosylated proteins located in the epithelial lining of several tissues and ducts. (amrita.edu)
Why does the observed western blot band size (i.e., molecular weight) differ from the expected band size for some proteins? (genetex.com)
Typically, SDS-PAGE allows electrophoretic separation of proteins in a sample by molecular weight (MW), with larger proteins migrating more slowly than smaller proteins. (genetex.com)
The most common example of this is an antibody produced against small molecular weight molecules or altered constituitive proteins. (cdc.gov)

Concentration Agarose concentration must be taken into account when selecting a marker. (wikipedia.org)

nature structural & molecular biology Abstract In the early stages of mitosis, cohesin is released from chromosome arms but not from centromeres. (cbinsights.com)
Due to advances in molecular biology, genetics, analytical chemistry, and other basic sciences, it is now possible to detect smaller amounts of analytes and contaminants and smaller biological changes, as well as to identify mechanisms at the cellular and molecular levels. (cdc.gov)

Hufbauer, M. & Akgül, B. Molecular mechanisms of human papillomavirus induced skin carcinogenesis. (nature.com)
Elevated excitability and its ac- and the molecular mechanisms of memory. (lu.se)
A reduction in the number of cells, the change in structure and functioning of organs, permanent change in DNA methylation, and in gene expression have also been considered to be molecular mechanisms responsible for fetal programming 1 . (bvsalud.org)

To address its ori- ters for Disease Control and Prevention, Atlanta, Georgia, for gin, we studied 47 clinical isolates from Russia and demon- molecular characterization. (cdc.gov)

Therefore, when used in gel electrophoresis, markers effectively provide a logarithmic scale by which to estimate the size of the other fragments (providing the fragment sizes of the marker are known). (wikipedia.org)
Nucleic acid molecular weight markers contain nucleic acids of several pre-defined molecular weights for use as size standards in gel electrophoresis. (thermofisher.com)
NEB also offers various conventional double-stranded DNA molecular weight markers with sizes up to 23 kb and markers for pulsed-field gel electrophoresis (PFGE) ranging from 1.5 to 1,018 kb. (neb.com)

as it relates to the cellular and molecular responses to damage [email protected]. (cdc.gov)

MUC glycoproteins: Potential biomarkers and molecular targets for cancer therapy. (amrita.edu)
Home Publications MUC glycoproteins: Potential biomarkers and molecular targets for cancer therapy. (amrita.edu)

Olfactory receptors are supposed to act not only as molecular sensors for odorants but also as cell recognition molecules guiding the axons of olfactory neurons to their appropriate glomerulus in the olfactory bulb. (jneurosci.org)
in the cilia recognizing odorous molecules from the environment and in the axons recognizing molecular cues in the olfactory bulb. (jneurosci.org)

To address this issue, a kit for Southern Blot analysis was developed in 1990, providing the first marker to combine target DNA and probe DNA. (wikipedia.org)

Loading protein markers of established size (e.g. (genetex.com)

A high sCD163 CSF/serum ratio in relation to molecular weight was found, strongly indicating local production in the CNS. (nih.gov)
[ 1 ] At present, serum creatinine, which is used to measure the glomerular filtration rate (GFR), is the most commonly used marker of renal function. (medscape.com)

RT-nested-PCR for the detection of a fragment of the ORF2 gene of hepatitis E virus, showing negative control (lane 1), a pooled fecal sample (7A, lane 2), slurry sample (7B, lane 3) M is 100 bp molecular weight marker (lane 4) and positive control (lane 5). (scielo.br)
NHANES testing for markers of infection with hepatitis viruses is used to determine secular trends in infection rates across most age and racial/ethnic groups and will provide a national picture of the epidemiologic determinants of these infections. (cdc.gov)

Use 20μl molecular weight marker per lane. (enzolifesciences.com)

With all six genotypes of citrus tested including Eureka lemon, a cultivar difficult to be transformed, our results demonstrate that the kn1 gene may provide an effective molecular tool to enhance genetic transformation efficiencies of various citrus varieties. (springer.com)
Gene therapy displacement of endogenous MCUb with a dominant-negative MCUb transgene (MCUb W246R/V251E ) in vivo rescued T2D cardiomyocytes from metabolic inflexibility and stimulated cardiac contractile function and adrenergic responsiveness by enhancing phospholamban phosphorylation via protein kinase A. We conclude that MCUb represents one newly discovered molecular effector at the interface of metabolism and cardiac function, and its repression improves the outcome of the chronically stressed diabetic heart. (diabetesjournals.org)
Molecular Polymorphisms of Vascular Endothelial Growth Factor Gene and Bronchopulmonary Dysplasia in Very Low Birth Weight Infants. (cdc.gov)

who have designed a pair of plasmids that together can be digested with one of two restriction enzymes to produce either 100-bp or 1000-bp DNA molecular weight markers. (plantae.org)

The pSer/pThr molecular weight marker contains rabbit muscle phosphoproteins isolated by Fe3+/IDA - affinity chromatography. (enzolifesciences.com)

An early problem in the development of markers was achieving high resolution throughout the entire length of the marker. (wikipedia.org)
A novel high molecular weight glutenin subunit encoded by the Glu-1B locus was identified in the French genotype Bagou, which we named 1B × 6.5. (mdpi.com)

To date, no compelling biological marker for exposure to Stachybotrys chartarum mycotoxins or other metabolites has been presented. (cdc.gov)

AFP and hCG have been commonly used as markers for hepatocellular carcinoma and trophoblastic disease, respectively. (aacc.org)

Disease markers 2022 2022 2793846. (cdc.gov)
Children with low birth weight, regardless of their social background, have an increased risk of presenting cardiovascular disease and type 2 diabetes compared to their peers of higher weight 10,11 . (bvsalud.org)
There is a direct relationship between low birth weight and chronic disease, even after adjusting for relevant confounding variables, including alcohol consumption, levels of physical exercise and socioeconomic status 10,11 . (bvsalud.org)

Molecular methods have been used to detect microorganisms that are impossible or difficult to culture. (bvsalud.org)
Molecular tests were used to detect pathogens. (who.int)

This article's uniqueness focuses on diagnosing Leishmaniasis, with particular attention to clinical manifestations and demographic factors when skin smear microscopy reveals a negative result and molecular tests are unavailable to the professional. (researchgate.net)
Although factors related to adult weight and life-style may be additional confounding factors, the association of low birth weight with chronic diseases seems to persist after exclusion of such factors. (bvsalud.org)

There are two common methods in which to construct a DNA molecular-weight size marker. (wikipedia.org)
This makes it more difficult to control the size of the fragments in the marker. (wikipedia.org)
More recently, another method for constructing DNA molecular-weight size markers is being employed by laboratories. (wikipedia.org)
As with experimental samples, the conditions of the gel can affect the molecular-weight size marker that runs alongside them. (wikipedia.org)

This is a clinical problem that affects around 7-15% of gestations3 and is associated with increased perinatal morbidity and mortality, as well as birth weight, which is understood to be an indicator of pregnancy conditions, and more specifically, intrauterine growth 4-8 . (bvsalud.org)

The markers are loaded in lanes adjacent to sample lanes before the commencement of the run. (wikipedia.org)

To assess the genetic diversity and structure of the bacteria obtained by traditional identification, the API Coryne test, or and its toxin, different molecular typing methods have been toxigenicity testing by the Elek test and PCR were detected. (cdc.gov)

Low birth weight, disproportion in head circumference, and the length and weight at birth are markers of alack in nutrients at certain stages of pregnancy 8,9 . (bvsalud.org)

Values to the left of the gel represent molecular weight (kDa) of bands. (avma.org)

Although the concept of molecular-weight markers has been retained, techniques of development have varied throughout the years. (wikipedia.org)
species can be clearly separated from each other by highly discriminating molecular techniques, such as multiplex PCR, multilocus sequencing typing, or multiple loci variable-number tandem repeat analysis. (cdc.gov)

AFP is a single-chain glycoprotein with a molecular weight of around 70 KDa and 4% carbohydrate content. (aacc.org)

Protein, DNA, and RNA markers with pre-determined fragment sizes and concentrations are commercially available. (wikipedia.org)

This slide summarizes how AFP and hCG function as tumor markers. (aacc.org)

These elements need to be taken into consideration when selecting a marker and when analyzing the final results on a gel. (wikipedia.org)

Stable incorporations of T-DNA into the citrus genome have been confirmed with both histochemical staining of GUS activity and molecular analyses. (springer.com)
The translated protein has a molecular weight of about 60 kD and exhibits NAD + -dependent deacetylase activity. (frontiersin.org)

A model is proposed where the relative levels of active antagonist (RsbV1) and switch-protein anti-sigma factor (RsbW) control the availability of σ 66 and subsequently act as a molecular 'throttle' for Chlamydia growth and development. (plos.org)

New inventions of molecular-weight markers are distributed in kits specific to the marker's type. (wikipedia.org)

This rate seems to be related to low birth weight and adverse events during pregnancy 12 . (bvsalud.org)

Anatomy16

Organisms16

Diseases8

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