After affinity isolation on immobilized BC3 antibody, MUC1 was partially deglycosylated by enzymatic treatment with alpha-sialidase/beta-galactosidase and fragmented by proteolytic cleavage with the Arg-C-specific endopeptidase clostripain. (nih.gov)
Muc1 might serve as a candidate for single chain antibody mediated targeted therapy in MUC1 expressing cancers. (sigmaaldrich.com)
Patients who maintained high immunoreactivity for anti-MUC1 antibody had a better prognosis, whereas those with an increase in anti-MUC3 immunoreactivity had a poorer prognosis, as judged by tumour size, serosal invasion, and metastasis. (bmj.com)
The crystal structure of the mucin monoclonal antibody SM3 complexed with a 13 amino acid MUC1 mucin peptide was determined. (wisconsin.edu)
Glycosylation9
The site-specific O-glycosylation of MUC1 tandem repeat peptides from secretory mucin of T47D breast cancer cells was analyzed. (nih.gov)
All five positions of the repeat peptide were revealed as O-glycosylation targets in the tumor cell, including the Thr within the DTR motif. (nih.gov)
In addition to a modification by glycosylation, the immunodominant DTR motif on T47D-MUC1 is altered by amino acid replacements (PAPGSTAPAAHGVTSAPESR), which were revealed in about 50% of PAP20 peptides. (nih.gov)
GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. (wikipedia.org)
1997). "Localization of O-glycosylation sites on glycopeptide fragments from lactation-associated MUC1. (wikipedia.org)
Upregulation of this gene, abnormal intracellular localization and varied glycosylation of Muc1 has been associated with carcinomas. (sigmaaldrich.com)
Engineering mammalian mucin-type O-glycosylation i. (analyteguru.com)
Mucin-type O-glycosylation is an important post-translational modification that confers a variety of biological properties and functions to proteins. (analyteguru.com)
O-Glycosylation was furthermore demonstrated on a tandem repeat of MUC16 and interferon α2b. (analyteguru.com)
Heavily glycosylated1
The Mucin 1 (MUC1) gene encodes a membrane bound glycosylated phosphoprotein, belonging to the family of mucins which are heavily glycosylated proteins. (sigmaaldrich.com)
Secretory3
Epithelial mucins can be classified into two distinct families: secretory (gel forming) and membrane bound. (bmj.com)
Ed mucin) is related with invasive proliferation of the tumors and poor outcome of the patients, whereas the buy 58-49-1 Expression of the MUC2 mucin (intestinal type secretory mucin) is related with the non-invasive proliferation of the tumors and a favorable outcome for the patients [4,5]. (calcium-channel.com)
In the cases with more than two histological types mixed in one lesion, each histological pattern was evaluated independently, according to the Japanese Classification.Ed mucin) is related with invasive proliferation of the tumors and poor outcome of the patients, whereas the expression of the MUC2 mucin (intestinal type secretory mucin) is related with the non-invasive proliferation of the tumors and a favorable outcome for the patients [4,5]. (calcium-channel.com)
Amino3
Muc1 is a type I transmembrane glycoprotein with a unique extracellular domain containing 20 amino acid variable number tandem repeat (VNTR) domain. (sigmaaldrich.com)
Glycosylated Mucin-1 (MUC1) peptide fragment containing amino acid sequence Pro126-Gly149 (24 AA in length) from the tandem repeat (TR) region of the MUC1 protein. (sussex-research.com)
First, MUC7 carries subexonic repeats that are 69 bp (23 amino acids) long and are copy number variable in humans 5 . (nature.com)
Peptide1
The high incidence of these replacements and their detection also in other cancer cell lines imply that the conserved tandem repeat domain of MUC1 is polymorphic with respect to the peptide sequence. (nih.gov)
MUC21
The expression of MUC1, MUC2, MUC3, MUC5AC, and MUC6 was investigated immunohistochemically in gastric carcinoma (n = 46) in relation to patient clinicopathological features. (bmj.com)
Monoclonal1
Monoclonal Anti-MUC1 (mouse IgG1 isotype) is derived from the hybridoma 1D1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with semi-purified MUC1 from pleural effusion of a breast cancer patient. (sigmaaldrich.com)
Gastric cancer1
In the present study, we examined the expression profiles of MUC4 as well as MUC1 in early gastric cancer tissues, and found that MUC4 and MUC1 expression in the early gastric cancers would become poor prognostic factors 18055761 by lymph vessel invasion, blood vessel invasion and lymph node metastasis. (calcium-channel.com)
Proteins1
Mucins are highly glycosylated proteins that serve a variety of roles in organisms from lubrication to cell-signaling to defense 7 . (nature.com)
Protein1
3, 4 Several tumour vaccines directed against MUC1 core protein, oligosaccharides, or MUC1 cDNAs have been shown to stimulate immune reactions in basic clinical trials to confer antitumour activity. (bmj.com)
Tumor Cells1
In the current study we show that designer glycopeptides containing the Thomsen-Freidenreich (TF) antigen (β-Gal-[1→3]-α-GalNAc- O -serine) are immunogenic in vivo and generate TF-specific CTL capable of recognizing a variety of tumor cells in vitro including a MUC1-expressing tumor. (silverchair.com)
Aberrant2
This study investigated the correlations between aberrant expression of mucins in gastric carcinoma and patient clinicopathological features. (bmj.com)
Aberrant (upregulated) expression of the MUC1 mucin has been seen in breast carcinomas and other neoplasms, such as colon and pancreatic cancers. (bmj.com)
Membrane bound1
Membrane bound mucins MUC1 and MUC3 appear to be associated with the development of gastric carcinoma. (bmj.com)
Carcinomas1
1, 2 Most studies of MUC1 expression in breast carcinomas have shown that increased membrane MUC1 mucin expression on the apical cell surface is associated with a better prognosis (reviewed in Rahn and colleagues 2 ), whereas circumferential staining in tumour cell cytoplasm is associated with a worse prognosis. (bmj.com)
Gene7
The MUC1 gene is mapped to human chromosome 1q22. (sigmaaldrich.com)
Mucin-1 kidney disease, previously described as medullary cystic kidney disease type 1 (MCKD1, OMIM 174000), is an autosomal dominant tubulointerstitial kidney disease recently shown to be caused by a single-base insertion within the variable number tandem repeat region of the MUC1 gene. (mit.edu)
The salivary MUC7 gene provides an opportunity for studying such variation, as it harbors copy number variable subexonic repeat sequences that encode for densely O-glycosylated domains (PTS-repeats) with microbe-binding properties. (nature.com)
Here, we focus on the salivary mucin-7 gene ( MUC7 ) to study the evolution and functional impact of subexonic repeats. (nature.com)
These repeats can be particularly well studied in this gene because the repeats are relatively short and few in number compared to other mucins. (nature.com)
Moreover, they demonstrated that phosphorylated NKCC2 levels rose in tandem with UMOD gene dosage. (medscape.com)
This disease, previously referred to as MCKD type 1, is due to a mutation in the variable-number tandem repeat region of the MUC1 (Mucin 1) gene. (medscape.com)
Mass Spectrometry2
We therefore developed and validated a mass spectrometry-based probe extension assay with a series of internal controls to detect the insertion event using 24 previously characterized positive samples from patients with mucin-1 kidney disease and 24 control samples known to be wild type for the variant. (mit.edu)
A chimeric YFP construct containing a 3.5 tandem repeat sequence of MUC1 was glycosylated with up to three and five GalNAc residues when co-expressed with GalNAc-T2 and a combination of GalNAc-T2 and GalNAc-T4, respectively, as determined by mass spectrometry. (analyteguru.com)
Mucin 1 lies on the tubular cell apical surface and has a role in signal transduction pathways. (medscape.com)
MUC42
namely, MUC3, MUC4, MUC12, MUC13, and the recently described mucin MUC17, which share a C-terminal structural domain that consists of two or three epithelial growth factor (EGF)-like regions, a transmembrane segment, and a short cytoplasmic tail. (bmj.com)
MUC4 was first reported as tracheobronchial mucin [7] and is a membrane-associated mucin [8]. (calcium-channel.com)
Epithelial1
In general, mucins have the unique function of protecting and lubricating epithelial surfaces, but in recent years they have also been implicated in additional diverse roles, such as growth, fetal development, epithelial renewal and differentiation, epithelial integrity, carcinogenesis, and metastasis. (bmj.com)
Specificity1
Validation results indicate an accurate and reliable test for clinically establishing the molecular diagnosis of mucin-1 kidney disease with 100% sensitivity and specificity across 275 tests called. (mit.edu)
Sequence1
The MAb 8G7 recognizes a tandem repeat sequence (STGDTTPLPVTDTSSV) of the human MUC4a subunit [14]. (calcium-channel.com)
Abstract1
From the abstract: 'Genome sequencing (GS) is one of the most comprehensive assays that interrogate single nucleotide variants, copy number variants, mitochondrial variants, repeat expansions, and structural variants in one assay. (cdc.gov)
Intracellular1
These findings may be linked to reports that MUC1 plays an important role in the impairment of cell-cell adhesion, the immune response, and/or altered intracellular signalling. (bmj.com)
Clinical2
Several studies have reported conflicting and inconclusive results concerning the clinical relevance of mucin expression in gastric carcinoma. (bmj.com)
Because of variable age of disease onset and often subtle signs and symptoms, clinical diagnosis of mucin-1 kidney disease and differentiation from other forms of hereditary kidney disease have been difficult. (mit.edu)
Study4
On a broader scale, our study highlights variable subexonic repeats as a primary source for modular evolutionary innovation that lead to rapid functional adaptation. (nature.com)
A recent study has shown that, despite the high intrinsic mutability of subexonic repeats, the vast majority of thousands of subexonic repeats remain strongly conserved among mammals 4 . (nature.com)
However, there is no study to our knowledge that specifically investigated the evolution of copy number variable subexonic repeats from their initial emergence as functional units to the adaptive constraints and mutational properties that contributed to their extant variation in primates. (nature.com)
Our previous study showed thatMUC4 and MUC1 Expression in Early Gastric CancersFigure 1. (calcium-channel.com)
High1
The causal insertion resides in a variable number tandem repeat region with high GC content, which has made detection by standard next-generation sequencing impossible to date. (mit.edu)
Breast1
semi purified MUC1 from pleural effusion of a breast cancer patient. (sigmaaldrich.com)
Cell1
Mucin 1 (Muc1) exhibits protective action by binding to pathogens and is also associated with cell signaling capacity. (sigmaaldrich.com)
Applications1
L'équipe travaille à la fois sur la conception de lectines artificielles que d'inhibiteurs dérivés de sucres afin de les utiliser en tant qu'outils pour cntribuer à la compréhension de processus biologiques que pour des applications en santé (diagnostic, anti-infectieux, anti-cancéreux). (univ-nantes.fr)