• He delineated the molecular basis of carbohydrate recognition by legume lectins and demonstrated the novel C-H...O/N hydrogen bond in protein-sugar interactions. (wikipedia.org)
  • His studies showed that the monomers of legume lectins have the necessary structural features for carbohydrate recognition and that oligomerization imparts them with topology necessary for their biologic activities such as agglutination, mitogenesis, etc. (wikipedia.org)
  • The conformation of a loop and its orientation with respect to the rest of the molecule in WBAII are, however, different from those in all the other legume lectins of known structure. (unboundmedicine.com)
  • Ligands resembling streptavidin, antibodies and lectins are used each to seize specifically-tagged targets and for the isolation of cells and biomolecules that naturally categorical the ligand binding companion. (gentaurpriceonline.com)
  • Discovery of O-Linked Carbohydrate on HIV-1 Envelope and Its Role in Shielding against One Category of Broadly Neutralizing Antibodies. (openwetware.org)
  • The Protein Data Bank (PDB) is a valuable resource for three-dimensional structural information on glycoproteins and protein-carbohydrate complexes. (iucr.org)
  • Our Con A-coated BioMag Plus microparticles present a handy means for isolating mannosyl- and glucosyl-containing glycoproteins and polysaccharides from serum or cell lysate, or for investigating different lectin / glycan-mediated processes. (gentaurpriceonline.com)
  • Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins . (lookformedical.com)
  • He genetically re-engineered carbohydrate specificities of lectins imparting T-antigen tumor recognizing ability to peanut agglutinin. (wikipedia.org)
  • He found a molten globule monomeric intermediate during the folding of peanut agglutinin that retained its carbohydrate binding ability - establishing 'wet' molten globule as an "on pathway" folding intermediate and characterized other early intermediates. (wikipedia.org)
  • He discovered the unusual quaternary structure of peanut agglutinin and a novel lectin fold in Jacalin as well as the structural basis of inactivation of ribosomes by gelonin. (wikipedia.org)
  • Nevertheless, we find putative ligands for the endothelial cell lectin, LSECtin. (ox.ac.uk)
  • Glycobiology is the study of the structure, function and biology of carbohydrates, also called glycans, which are widely distributed in nature. (neb.com)
  • EndoBI-1-released N-glycans were quantified by a phenol-sulphuric total carbohydrate assay and the resultant N-glycan structures monitored by nano-LC-Chip-Q-TOF MS. The kinetic parameters and structural characterization of glycans released suggest EndoBI-1 can facilitate large-scale release of complex, bioactive glycans from a variety of glycoprotein substrates. (omicsdi.org)
  • Griffithsin (GRFT), an algae-derived lectin, is one of the most potent viral entry inhibitors discovered to date. (mdpi.com)
  • In-Silico Exploration of Antiviral Lectin Griffithsin. (openwetware.org)
  • Crystal structure and carbohydrate analysis of Nipah virus attachment glycoprotein: a template for antiviral and vaccine design. (ox.ac.uk)
  • Here we present the structural analysis of the protein and carbohydrate components of the unbound viral attachment glycoprotein of NiV glycoprotein (NiV-G) at a 2.2-A resolution. (ox.ac.uk)
  • Productive folding of the protein moiety is facilitated during this period by the calnexin/calreticulin cycle in mammalian cells, which relies on G1M9-specific lectin-type chaperones (calnexin and calreticulin) associated with the oxidoreductase ERp57, and UDP-glucose:glycoprotein glucosyltransferases 1 and 2, which are capable of re-adding glucose to M9 if the protein moiety is not yet folded. (elifesciences.org)
  • Knowledge of the three-dimensional structures of the carbo-hydrate molecules is indispensable for a full understanding of the molecular processes in which carbohydrates are involved, such as protein glycosylation or protein-carbohydrate interactions. (iucr.org)
  • Glycosylation is classified by the way the carbohydrate chain is linked to the protein. (iucr.org)
  • This difference reflects some structural heterogeneity in the carbohydrate moiety of the HLA-CW2 antigen. (ox.ac.uk)
  • Polysaccharides are complex carbohydrates composed of long chains of monosaccharide units, with important functions in the body including energy storage, structural support, and immune response. (lookformedical.com)
  • He is known for his work on lectin structure and interactions, orientation and dynamics of cell surface carbohydrate receptors and protein folding, diabetes, antimalarials and anti-cancer agents based on curcumin, flavonoids, etc. (wikipedia.org)
  • Surolia has elucidated the role of glycosphingolipids as biological receptors through studies on lectin-glycolipid interactions using liposomes. (wikipedia.org)
  • Researchers in the Netherlands, Cuba, and Italy have made important discoveries about the carbohydrate-mediated binding of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein to host cell lectin receptors and host lung microbiota. (news-medical.net)
  • He discovered novel blood group and tumor antigen specific lectins and made original contribution towards elucidation of the energetics and mechanism of protein-sugar recognition. (wikipedia.org)
  • PPL1 has two tandem carbohydrate recognition domain (CRD) structures in its sequence which has sequence homology with rhamnose-binding lectins (RBLs) from various fish eggs and a galactose-binding lectin (SUEL) from sea urchin eggs [24]. (cancer-ecosystem.com)
  • However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition. (lookformedical.com)
  • These observations spotlight the unique functions diversity and molecular evolution of this lectin family involved in the mollusk shell formation. (cancer-ecosystem.com)
  • Previously we isolated an 18 kDa lectin (which we now refer to as PPL1) from the mantle of large-winged pearl shells and determined its primary structure and molecular properties [23]. (cancer-ecosystem.com)
  • Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. (lookformedical.com)
  • Also, whereas essentially all of the HLA-A and B antigens and about half of the HLA-CW2 antigen were adsorbed strongly by Lens culinaris lectin-Sepharose, the remaining HLA-CW2 antigen was bound much more weakly and did not require sugar for elution. (ox.ac.uk)
  • Tumors express embryonic carbohydrate antigens called tumor-associated carbohydrate antigens (TACA). (silverchair.com)
  • Modelling based on the crystal structures provides a rationale for the specificity of the lectin, which is very different from that of WBAI, for the H-antigenic determinant responsible for O blood group reactivity. (unboundmedicine.com)
  • This article gives an overview of the information that can be obtained from individual PDB entries and from statistical analyses of sets of three-dimensional structures, of typical problems that arise during the analysis of carbohydrate three-dimensional structures and of the validation tools that are currently available to scientists to evaluate the quality of these structures. (iucr.org)
  • Unfortunately, many carbohydrate moieties in the PDB contain inconsistencies or errors. (iucr.org)
  • We extend our structural characterization of NiV-G with mass spectrometric analysis of the carbohydrate moieties. (ox.ac.uk)
  • These data suggest that small carbohydrate moieties may "fit" in the variable region of the TcR. (silverchair.com)
  • For structural analysis of asparagine-linked carbohydrates, sugars are released from the protein backbone by enzymes such as PNGase F ( NEB #P0704 ). (neb.com)
  • Enzymes that catalyze the transfer of mannose from a nucleoside diphosphate mannose to an acceptor molecule which is frequently another carbohydrate. (lookformedical.com)
  • Les interactions sucres/protéines (enzymes, lectines) sont au cœur de la communication cellulaire tant extracellulaire (interactions cellule-cellule, cellule-matrice, hôte-pathogène) qu'intracellulaire (GlcNAcylation en tant que modification post-traductionnelle). (univ-nantes.fr)
  • He showed the existence of co-operativity in lectin-multimeric sugar interactions. (wikipedia.org)
  • TY - JOUR T1 - Carbohydrate specificity and salt-bridge mediated conformational change in acidic winged bean agglutinin. (unboundmedicine.com)
  • The effect of macromolecular crowding on the conformational features and carbohydrate binding properties of CIA17, a PP2-type lectin, was investigated employing polymeric dextrans D6, D40, and D70 (Mr â ¼ 6, 40, and 70 kDa, respectively) as crowders. (bvsalud.org)
  • Therefore, carbohydrate chains are usually displayed as a tree-like two-dimensional graph. (iucr.org)
  • Understanding the nuances of prefusion and postfusion conformations shed light on viral cell entry and may, in turn, guide the development of vaccines and drugs. (news-medical.net)
  • Con A binding is mediated by metallic ions, which stabilize is conformation. (gentaurpriceonline.com)
  • Every binding website requires calcium and manganese ions, and use of buffers with EDTA or different metallic chelators will lead to a lack of carbohydrate binding skill. (gentaurpriceonline.com)
  • The subunit structure and dimerisation of the lectin are similar to those of the basic lectin from winged bean (WBAI) and the lectin from Erythrina corallodendron (EcorL). (unboundmedicine.com)
  • One can define several major GSL series based on their internal core carbohydrate structure. (springer.com)
  • Furthermore it was found that trehalose which is usually specific recognizing carbohydrates for PPL2A and is abundant in the secreted fluid of mantle functions as a regulatory factor for biomineralization via PPL2A. (cancer-ecosystem.com)
  • Klinische studies tonen echter aan dat er dagelijks minstens 3,6 gram oplosbare vezels nodig is om het risico op hart- en vaatziekten te verminderen. (sohf.nl)
  • It also leads to a qualitative explanation for the thermodynamic data on sugar-binding to the lectin, with special emphasis on the role of a tyrosyl residue in the variable loop in the sugar-binding region in generating the carbohydrate specificity of WBAII. (unboundmedicine.com)
  • In the present study two jacalin-related lectins named PPL2A and PPL2B were isolated from the mantle and secreted fluid of protease I and V8 protease were purchased from Wako Pure Chemical (Osaka PP1 Analog II, 1NM-PP1 Japan). (cancer-ecosystem.com)