Laminin membrane proteins. Medical search. Frequent questions

Amino acid sequence of mouse nidogen, a multidomain basement membrane protein with binding activity for laminin, collagen IV and cells. (unifi.it)
Binding sites for laminin and collagen IV are present on the C-terminal globule but not yet precisely localized. (unifi.it)
No specific or efficient treatment exists for Alport syndrome, an X-linked hereditary disease caused by mutations in collagen type IV, a crucial component of the glomerular basement membrane. (hindawi.com)
We are currently working on a novel model of nephrotic syndrome resulting from a point mutation in laminin alpha5, osteophyte formation in a model of osteoarthritis with defective collagen 1 alpha 2, and the effects of an aggrecan mutation on joint deterioration and adiposity. (brookes.ac.uk)
Other than water, the mesoglea is composed of several substances including fibrous proteins like collagen and heparan sulphate proteoglycans. (pearltrees.com)
Laminin and type IV collagen are the two principal components of the basement membrane. (biogenex.com)
Because type IV collagen and laminin are proteins found exclusively in the basement membrane, the antibodies to these two proteins selectively and specifically recognize basement membrane components. (biogenex.com)
Although the mechanisms mediating the effects of the extracellular matrix (ECM) on breast carcinoma development in vivo are largely unknown, contributing factors may be adhesion mediated signaling and mechanical signals imparted on mammary epithelial cells from surrounding type-I collagen-rich stroma, either directly or across basement membrane proteins. (biomedcentral.com)
Immunohistochemical analysis of laminin and various types of collagen. (biopticka.cz)
Distribution of type IV collagen and laminin. (biopticka.cz)
BMs are specialized structures that underlie epithelia and surround organs and mainly comprise collagen IV, laminins, and heparan sulfate proteoglycans (HSPG). (nature.com)
It boosts production of basal membrane proteins, including collagen and laminins. (rituel-beaute.ca)
This form of the disease is the result of abnormalities in collagen (a type of connective tissue) and laminin (a protein found in the basement membrane which helps to hold cells and tissues together). (entclinic.com.au)
Collagen IV, pan-laminin, perlecan and laminin-α5 in the islet BM were significantly digested by enzyme treatment. (ox.ac.uk)
Collagen IV and pan-laminin were present in the disorganized BM of isolated islets, yet a significant reduction in pan-laminin was seen during the initial 24 h culture period. (ox.ac.uk)
Immunological investigations consisted of direct immunofluorescence (IF), indirect IF with intact and salt-split canine gingival, and salt-split normal or laminin-332-deficient human skin, immunoblotting with purified human laminin-332 and immunoblotting with recombinant NC1 domain of human collagen VII. (morulaivf.com)
Epidermolysis bullosa acquisita is caused by antibodies targeting type VII collagen, the major component of anchoring fibrils that connect the basement membrane to dermal structures. (medscape.com)
[ 3 ] IgG autoantibodies specific for anchoring fibrils (type VII collagen) of the skin basement membrane have a heterogeneous subclass and light-chain composition, and their complement-activating capacities do not correlate with the inflammatory phenotype. (medscape.com)
Initial studies demonstrated that rabbit antimouse type VII collagen antibodies, as well as rabbit antibodies raised against human recombinant protein type VII collagen, were able to induce blisters in mice. (medscape.com)
Induction of an active model of autoimmunity against type VII collagen in a hairless mouse strain (SKH1) revealed that regulatory T cells, which have been identified for their ability to inhibit the development of autoimmune diseases, do not inhibit the development of autoantibodies against the self-protein mouse-type VII collagen. (medscape.com)

Laminin subunit alpha-2 is a protein that in humans is encoded by the LAMA2 gene. (wikipedia.org)
This gene provides instructions for making a part (subunit) of certain members of a protein family called laminins. (medlineplus.gov)
Most LAMA2 gene mutations that cause the severe, early-onset form of LAMA2 -related muscular dystrophy result in the absence of functional laminin alpha-2 subunit. (medlineplus.gov)
Mutations that cause the milder, later-onset form usually result in a reduction (deficiency) of functional laminin alpha-2 subunit. (medlineplus.gov)
Deficiency or absence of the laminin alpha-2 subunit results in a corresponding lack of laminin 2 and laminin 4, reducing the strength and stability of muscle tissue and leading to the signs and symptoms of LAMA2 -related muscular dystrophy. (medlineplus.gov)
This protein is a beta subunit laminin, which together with an alpha and a gamma subunit, forms laminin-5. (lsbio.com)
Laminin consists of a 220 kD subunit, which is disulfide-linked into larger complexes or even into the structural scaffolding of the basement membrane itself. (biogenex.com)
ELAV - like1 , methyltransferase like 7A , dolichyl-phosphate mannosyltransferase , laminin subunit beta-1 , gremlin 1 , BCL6 corepressor-like 1 , and three genes of unknown identity. (biomedcentral.com)

This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). (wikipedia.org)
A CMD Subtype that results from gene defects in the laminin-alpha2 gene affecting the production of merosin protein in the muscle cell. (cmdir.org)
Laminin is a protein that produces merosin for the extracellular matrix. (cmdir.org)

Laminin, an extracellular matrix protein, is a major component of the basement membrane. (wikipedia.org)

The names 'acquired junctional epidermolysis bullosa', 'anti-laminin-332 mucous membrane pemphigoid (MMP)' and 'mixed auto-immune subepidermal blistering dermatosis' are proposed for dogs with clinical signs reminiscent of EBA, MMP or BP respectively. (morulaivf.com)
Molecular diagnosis of anti-laminin 332 (epiligrin) mucous membrane pemphigoid. (fmu.ac.jp)

The initial attachment process of CEF on fibronectin and laminin substrata was not influenced by preincubating these cells with antibodies against chicken gizzard 5′-nucleotidase. (morulaivf.com)

We have determined in F9 cells that dissociating GalTase adhesion by galactosylation of and release of the consociate glycoproteins induces differentiation, as defined by increased laminin synthesis. (tmc.edu)

This is the first mouse model demonstrating that mutations in this basement membrane protein results in chronic renal disease and confirms current findings from patient sequencing. (brookes.ac.uk)
Aggrecan is an essential protein present in cartilage and we have identified a point mutation in this protein which better models phenotypes seen in patients with mutations in aggrecan such as aggressive osteoarthritis and short stature. (brookes.ac.uk)
PMID: 25486376 Mutations in Biosynthetic Enzymes for the Protein Linker Region of Chondroitin/Dermatan/Heparan Sulfate Cause Skeletal and Skin Dysplasias . (symptoma.com)

In the present study, we explored the structural aspect of HomA and HomB proteins using various computational, biophysical and small-angle X-ray scattering (SAXS) techniques. (nature.com)
Our study provides essential structural information of unexplored proteins of the Hom family that can help in a better understanding of H. pylori pathogenesis. (nature.com)
3] They are composed of a family of related proteins sharing common structural and sequence features. (pearltrees.com)
Lamins, also known as nuclear lamins are fibrous proteins in type V intermediate filaments, providing structural function and transcriptional regulation in the cell nucleus. (cmdir.org)
This type of the condition is caused by genetic defects in keratin, a protein which is an important structural component of the skin. (entclinic.com.au)

The laminins can self-assemble, bind to other matrix macromolecules, and have unique and shared cell interactions mediated by integrins, dystroglycan, and other receptors. (embl.de)

They are a major component of the basal lamina (one of the layers of the basement membrane), a protein network foundation for most cells and organs. (pearltrees.com)
The laminins are an important and biologically active part of the basal lamina, influencing cell differentiation, migration, and adhesion. (pearltrees.com)
In contrast, in the submucosa, laminin was exclusively found in the basal lamina of blood vessels where many adherent mast cells were seen. (bmj.com)
One side of the cell sheet is called 'apical', the other 'basal', and each contains distinct proteins. (news-medical.net)
Immune disturbance as well as destruction of basal epidermal cells and base membrane by GVHD may result in the induction of autoimmune blistering diseases with unusual clinical and laboratory manifestations. (morulaivf.com)
Se localiza en la membrana basal de la lámina lúcida y funciona uniendo las células epiteliales a las membranas basales. (bvsalud.org)

We sought to determine whether immune reactivity occurs between anti-SARS-CoV-2 protein antibodies and human tissue antigens, and whether molecular mimicry between COVID-19 viral proteins and human tissues could be the cause. (frontiersin.org)
We applied both human monoclonal anti-SARS-Cov-2 antibodies (spike protein, nucleoprotein) and rabbit polyclonal anti-SARS-Cov-2 antibodies (envelope protein, membrane protein) to 55 different tissue antigens. (frontiersin.org)
We found that SARS-CoV-2 antibodies had reactions with 28 out of 55 tissue antigens, representing a diversity of tissue groups that included barrier proteins, gastrointestinal, thyroid and neural tissues, and more. (frontiersin.org)
In this mechanism, antibodies formed against SARS-CoV-2 would also bind to human tissue proteins leading to autoimmune reactivity. (frontiersin.org)
Polyclonal and monoclonal antibodies against chicken gizzard 5 '-nucleotidase inhibit the spreading process of chicken embryonic fibroblasts on laminin substratum. (morulaivf.com)
Polyclonal and monoclonal antibodies raised against chicken gizzard 5′-nucleotidase were tested in adhesion assays of embryonic chicken fibroblasts (CEF) for their ability to interfere with the adhesion process of these cells on either laminin or fibronectin substrata. (morulaivf.com)
Laminin-332 polypeptides are targeted by auto-antibodies in human patients with mucous membrane (cicatricial) pemphigoid or, more rarely, subepidermal vesicular diseases that resemble epidermolysis bullosa acquisita (EBA) or bullous pemphigoid (BP). (morulaivf.com)
In all nine dogs, IgG auto-antibodies detected purified human laminin-332 by immunoblotting. (morulaivf.com)
Immunoblot assays using human epidermal extracts and BP180 NC16a domain recombinant protein confirmed the presence of IgG antibodies against BP180 and recombinant BP180 NC16a domain protein. (morulaivf.com)
however, rabbit antibodies raised against the recombinant proteins encoded by the gene of mouse bullous pemphigoid antigen 2 (BP180) are capable of inducing blisters in newborn mice in a complement-dependent manner. (medscape.com)
[ 4 ] These antibodies compromise the strength of the basement membrane zone, leading to skin fragility and trauma-induced blister formation. (medscape.com)

This layer is called the basement membrane. (entclinic.com.au)

β-Cells depend on the islet basement membrane (BM). (nature.com)
Key Matrix Proteins Within the Pancreatic Islet Basement Membrane Are Differentially Digested During Human Islet Isolation. (ox.ac.uk)
The islet basement membrane (BM) influences islet function and survival and is a critical marker of islet integrity following rodent islet isolation. (ox.ac.uk)

Functional analyses indicated that some of the genes induced by dragonfly larvae caused an increase in laminins necessary for cell adhesion in the extracellular matrix. (biomedcentral.com)
Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. (joplink.net)
A nanoscale modular design strategy was employed to synthesize six engineered, recombinant proteins intended to mimic aspects of the extracellular matrix proteins fibronectin, laminin, and elastin as well as the cell-cell adhesive protein neural cell adhesion molecule. (frontiersin.org)
P-selectin, soluble endothelial protein C receptor, soluble thrombomodulin, plasminogen activator inhibitor 1, ADAMTS-13, von Willebrand factor, tissue factor, soluble intercellular adhesion molecule 1, and vascular cell adhesion molecule 1 were more elevated in LF patients than in controls. (cdc.gov)

Whereas the pemphigus subset of diseases is mediated by autoantibodies that target the extracellular skin components that link one epidermal cell to another, the pemphigoid subset is mediated by autoantibodies that target the extracellular skin components that link the skin basement membrane components either to the lowermost layer of epidermal cells or to the dermal components. (medscape.com)

Laminin proteins are made of three different subunits called alpha, beta, and gamma. (medlineplus.gov)
Each laminin is a heterotrimer assembled from alpha, beta and gamma chain subunits, secreted and incorporated into cell-associated extracellular matrices. (embl.de)

It is well known that the adsorption of proteins onto biomaterial surfaces modulates the cellular interaction with these surfaces. (wikigenes.org)

Particularly in the Dowling-Maera subtype, the blisters may affect the mucous membrane lining the mouth. (entclinic.com.au)
A group of autoimmune blistering diseases affecting primarily the mucous membranes is termed mucous membrane pemphigoid (MMP) (also termed cicatricial pemphigoid ). (medscape.com)

We make use of a highly flexible multi-step cloning strategy in order to allow for the rapid synthesis of new recombinant proteins that can introduce many different biofunctionalities. (frontiersin.org)

Epidermolysis bullosa acquisita (EBA) is a chronic autoimmune subepidermal blistering disease of the skin and mucus membranes. (medscape.com)

HomB protein contributes to the colonization and persistence of H. pylori , and the presence of homB genes affects the number of bacteria adhering to the host cells. (nature.com)
the tadpoles showed reduced expression of cα(E)-catenin , small muscle protein, dystrophin , and myosin light chain genes. (biomedcentral.com)
In contrast, culturing on Laminin-421 induced the expression of β-cell genes, including Ins1 , MafA , and Glut2 , and significantly improved glucose-stimulated insulin secretion. (nature.com)

Further, biophysical experiments suggested that HomA and HomB are dimeric and most likely the cysteine residues present on surface-exposed loops participate in protein-protein interactions. (nature.com)
Therefore, this engineered protein adsorption approach allows for the facile preparation of tunable, quantifiable, and reproducible surfaces for in vitro studies of cell-ligand interactions and for potential application as coatings on neural implants. (frontiersin.org)
The structure of a Ca(2+)-binding epidermal growth factor-like domain: its role in protein-protein interactions. (embl.de)

Compared to ECs, pancreatic pericytes produce significantly higher levels of α2 and α4 laminin chains, which constitute the peri-islet and vascular BM. (nature.com)
Laminin is a T-shaped, trans-membrane protein composed of 3 chains , whose role is to anchor the dermis to the epidermis. (basf.com)
The different laminin chains share a 600-residue domain I/II which oligomerises into a rod-like coiled-coil structure forming the long arm of laminins. (embl.de)
Laminin IV domain is also found in the perlecan protein, an integral component of basement membranes, which serves also as an attachment substrate for cells, but it is not found in short laminin chains (alpha4 or beta3). (embl.de)
Laminin-332 (laminin-5) is a basement membrane heterotrimeric protein composed of alpha-3, beta-3 and gamma-2 laminin chains. (morulaivf.com)

Also functions as a cell surface receptor for laminin. (joplink.net)
Endothelial protein C receptor, thrombomodulin, intercellular adhesion molecule 1, plasminogen activator inhibitor 1, D-dimer, and hepatocyte growth factor were higher in fatal than nonfatal LF cases. (cdc.gov)

By specifically stimulating laminin synthesis, BasalineTM effectively smoothes out creases at the skin surface. (basf.com)
By specifically stimulating laminin synthesis, Basaline TM effectively smoothes out creases at the skin surface. (basf.com)
Basaline TM , a relief smoother and activator of laminin, reactivates the synthesis of laminin in an intelligent manner because it stimulates only those systems with a laminin deficit, a condition unique to mature skin. (basf.com)

In isolated islets, laminin-α5 (found in both layers of the duplex BM) and perlecan were lost entirely, with no restoration evident during culture. (ox.ac.uk)

An Illustration of the Laminin-111 complex depicting the domain organization Laminins are high-molecular weight (~400 to ~900 kDa) proteins of the extracellular matrix. (pearltrees.com)
Laminin is a large molecular weight glycoprotein present only in basement membranes in almost every animal tissue. (embl.de)

Laminin 2 and laminin 4 play a particularly important role in the skeletal muscles. (medlineplus.gov)

Multidomain proteins of the extracellular matrix (ECM) play an important role in development and maintenance of cellular organization and in tissue repair. (embl.de)

Thrombospondin 1, abbreviated as THBS1, is a protein that in humans is encoded by the THBS1 gene. (pearltrees.com)
Several ECM proteins such as laminin, tenascin and thrombospondin contain domains with homology to epidermal growth factor (EGF) and exhibit growth promoting activity. (embl.de)

These observations establish laminin-332 as a novel basement membrane antigen in dogs with autoimmune blistering diseases with variable clinical phenotypes. (morulaivf.com)

The protein consists of some 1217 amino acid residues and a 28-residue signal peptide. (unifi.it)
Hom (Helicobacter outer membrane) family of OMPs in H. pylori consists of four members (HomA, B, C and D). In the H. pylori genome jhp 0870 open-reading frame (ORF) that codes for HomB outer membrane protein is associated with many stomach diseases and is a novel co-marker for peptic ulcer disease (PUD) 9 , 10 . (nature.com)

In five dogs, IgG variably recognized the basement membrane of laminin-332-deficient human skin (three dogs negative, two dogs positive). (morulaivf.com)

Two transcript variants encoding different proteins have been found for this gene. (wikipedia.org)
Multiple alternatively spliced transcript variants that encode the same protein have been found for this gene. (lsbio.com)

GalTase expression at both the transcription and protein levels were differentially regulated during the cell cycle, with surface expression greatest in the G1 phase. (tmc.edu)
Fakan F, Skálová A, Kuntscherová J: Expression of bcl-2 protein in distinguishing benign from malignant lymphoid aggregates in bone marrow biopsies. (biopticka.cz)
Whereas α2 laminins promoted islet cell clustering, they did not affect gene expression. (nature.com)
Increased mRNA expression of a laminin-binding protein in human colon carcinoma: complete sequence of a full-length cDNA encoding the protein. (joplink.net)

Microhematuria and subsequently proteinuria are hallmarks of kidney involvement, which are due to primary basement membrane alterations that mainly cause endothelial thrombosis and podocyte contraction and ulterior irreversible detachment. (hindawi.com)
The impaired homeostasis and platelet dysfunction implicate alterations in the protein C pathway, which might contribute to the loss of endothelial barrier function in fatal infections. (cdc.gov)

Integrin alpha 3, also known as CD49c and VLA-3 alpha, is a type I membrane protein that heterodimerizes with Integrin beta 1. (rndsystems.com)

We further found that the pericytic laminin isoforms differentially regulate mouse β-cells. (nature.com)

Laminins are found in an intricate lattice of proteins and other molecules that forms in the spaces between cells (the extracellular matrix). (medlineplus.gov)
The laminins attach (bind) to other proteins in the extracellular matrix and in the membrane of muscle cells, which helps maintain the stability of muscle fibers. (medlineplus.gov)
All mast cells in the muscularis propria were colocalised with patches of laminin. (bmj.com)
The colocalisation with laminin indicates a mechanism of interaction between smooth muscle cells and mast cells that may be important in the role of mast cells in the process of fibrosis. (bmj.com)
Fibrosis is characterised by an excessive production of extracellular matrix components, and cells in the tissue are constantly interacting with these extracellular matrix proteins. (bmj.com)
The peri-islet membrane, which encapsulates the islets, separates the endocrine cells from the exocrine pancreas and serves as a barrier from immune cell infiltration to the islets. (nature.com)
Thus, human endocrine cells are not in direct contact with the vascular BM components but with the invaginated peri-islet membrane. (nature.com)
As confirmation that ligand density in these engineered systems impacts neuronal cell behavior, we demonstrate that increasing the density of fibronectin-derived RGD ligands on coated surfaces while maintaining uniform protein surface coverage results in enhanced neurite extension of PC-12 cells. (frontiersin.org)
However, the subsequent spreading process of these cells was found to be inhibited for at least 2 h on a laminin substratum. (morulaivf.com)
Spreading-arrested and rounded CEF do not develop prominent intracellular stress-fibers like control cells, instead they seem to concentrate their available actin in areas of presumptive initial contact with the laminin substratum. (morulaivf.com)
It is localized in the basement membrane lamina lucida and functions to bind epithelial cells to the basement membrane. (bvsalud.org)

They are classified into 5 groups according to substrate specificity and internal homology: collagenases, gelatinases, stromelysins, membrane-type MMPs, and others, including matrilysins 8 . (bvsalud.org)

Ectoin® can be called an "anti-stress" molecule as it helps to protect the cellular membranes from environmental hazards. (rituel-beaute.ca)

A sequence of about thirty to forty amino-acid residues long found in the sequence of epidermal growth factor (EGF) has been shown [ ( PUBMED:2288911 ) ( PUBMED:6334307 ) ( PUBMED:6607417 ) ( PUBMED:3282918 ) ] to be present, in a more or less conserved form, in a large number of other, mostly animal proteins. (embl.de)
Various diverse extracellular proteins possess Ca(2+)-binding epidermal growth factor (EGF)-like domains, the function of which remains uncertain. (embl.de)
Several complementary DNA clones encoding related HRGs were identified, all of which are similar to proteins in the epidermal growth factor family. (embl.de)

Scarring also affects the internal mucous membranes, which can lead to strictures (narrowing) of internal passages, such as the oesophagus and urethra. (entclinic.com.au)

investigated pathogenesis and immunological response caused by HomB protein in clinical patients and 190 H. pylori strain isolated from patients with peptic ulcer disease (PUD) or gastritis were evaluated for the clinical importance of homB . (nature.com)

The sympathetic nervous system regulates this function through β-adrenergic stimulation of brown mature adipocytes' dissipation of energy in the form of heat mediated by mitochondrial uncoupling protein-1 (UCP-1) activation. (springer.com)

Cross-reactivity occurs when amino acid sequence homology exists between a pathogen and self-tissue proteins ( 1 ). (frontiersin.org)

Anatomy46

Organisms10

Chemicals and Drugs109

Analytical, Diagnostic and Therapeutic Techniques and Equipment32

Phenomena and Processes59

Disciplines and Occupations1

Technology, Industry, Agriculture7

Information Science3

Health Care1

Collagen20

Subunit8

Merosin3

Extracellular matrix1

Anti-laminin-3322

Fibronectin and laminin1

Increased laminin synthesis1

Mutations3

Structural5

Receptors1

Basal6

Antibodies11

Called the basement membrane1

Islet basement membrane3

Cell adhesion4

Basement membrane components1

Subunits2

Adsorption of proteins1

Mucous membrane2

Recombinant proteins1

Epidermolysis1

Genes3

Interactions3

Chains5

Receptor2

Synthesis3

Perlecan1

Molecular2

Skeletal1

Multidomain1

Thrombospondin2

Autoimmune1

Consists2

Deficient1

Found for this gene2

Expression4

Alterations2

Integrin1

Differentially1

Cells11

MMPs1

Cellular1

Epidermal3

Strictures1

Immunological1

Mitochondrial1

Reactivity1