Also, infusion with kainic acid in the hippocampus of animals results in major damage of pyramidal neurons and subsequent seizure activity. (wikipedia.org)
Using immunocytochemistry and ELISA, we investigated the production of interleukin (IL)-1β in the rat hippocampus after focal application of kainic acid inducing electroencephalographic (EEG) seizures and CA3 neuronal cell loss. (jneurosci.org)
Domoic acid has been associated with necrosis of the glutamate-rich hippocampus and amygdala in autopsied cases. (medscape.com)
miR-22-3p (hereafter miR-22), a conserved miRNA that is expressed throughout the body, including the brain [ 14 , 15 ], was previously identified among upregulated miRNAs within the mouse hippocampus contralateral to the epileptogenic zone in the intraamygdala kainic acid model of status epilepticus [ 16 ]. (researchsquare.com)
The third was an investigation of the excitatory amino acid receptors responsible for epileptiform bursting activity in 2 animal models of epilepsy: a chronic model of temporal lobe epilepsy, involved prior lesioning of the hippocampus with kainic acid, and an acute model which involved application of the GABA-A receptor antagonist, bicuculline. (soton.ac.uk)
Seizures8
Kainic acid is a potent central nervous system excitant that is used in epilepsy research to induce seizures in experimental animals, at a typical dose of 10-30 mg/kg in mice. (wikipedia.org)
In addition to inducing seizures, kainic acid is excitotoxic and epileptogenic. (wikipedia.org)
Kainic acid induces seizures via activation of kainate receptors containing the GluK2 subunit and also through activation of AMPA receptors, for which it serves as a partial agonist. (wikipedia.org)
Scholars@Duke publication: Seizures, cell death, and mossy fiber sprouting in kainic acid-treated organotypic hippocampal cultures. (duke.edu)
We report here that that application of the convulsant, kainic acid, to organotypic hippocampal explant cultures induces seizures, neuronal cell death, and subsequent dramatic mossy fiber sprouting with a similar laminar preference and time-course to that seen in intact animals. (duke.edu)
Brainstem activity, apnea, and death during seizures induced by intrahippocampal kainic acid in anaesthetized rats. (ox.ac.uk)
Journal Article] Limb-clasping, cognitive deficit and increased vulnerability to kainic acid - induced seizures in neuronal GPI anchor deficiency mouse models. (nii.ac.jp)
Differential 24h responsiveness of Prox1-expressing precursor cells in adult hippocampal neurogenesis to physical activity, environmental enrichment, and kainic acid-induced seizures. (mpg.de)
Receptors5
Kainic acid is a potent neuroexcitatory amino acid agonist that acts by activating receptors for glutamate, the principal excitatory neurotransmitter in the central nervous system. (wikipedia.org)
Kainic acid is an agonist for kainate receptors, a type of ionotropic glutamate receptor. (wikipedia.org)
Kainic acid is a direct agonist of the glutamic kainate receptors and large doses of concentrated solutions produce immediate neuronal death by overstimulating neurons to death. (wikipedia.org)
N ociceptive primary afferent C-fibers express a subset of glutamate receptors that are sensitive to kainic acid. (biopsychiatry.com)
Excitotoxicity refers to the neurotoxic effect of excitatory amino acids in the presence of excessive activation of postsynaptic receptors. (medscape.com)
Hippocampal4
The cellular and molecular determinants required for kainic acid-induced cell death and subsequent mossy fiber reorganization thus appear to be intrinsic to the hippocampal slice preparation, and are preserved in culture. (duke.edu)
In this study, the effects of 1-BCP on excitatory amino acid agonist-induced [3H]NE release in rat hippocampal slices were determined. (nih.gov)
We have shown previously, that mice lacking tumor necrosis factor-α (TNF-α) receptor 1 (TNFR1) exhibit greater hippocampal neurodegeneration, suggesting that TNFR1 may be protective in kainic acid (KA)-induced neurotoxicity. (uaeu.ac.ae)
Kainic acid lesions were also produced in either hippocampal field 1 day after training and retention measured 8 days later. (karger.com)
Domoic acid8
In 2019, Chekan et al were able to use bioinformatic tools to look for domoic acid gene homologs in the seaweed Digenea simplex. (wikipedia.org)
neuroscience research neurodegenerative agent modeling of epilepsy modeling of Alzheimer's disease Dihydrokainic acid Domoic acid Kainate receptor Carlson NR (2013). (wikipedia.org)
Domoic acid (DA) is structurally similar to the excitatory neurotransmitter glutamate. (medscape.com)
Domoic acid is produced by the diatom Nitzschia pungens . (medscape.com)
When administered i.p., 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), an (R,S)-alpha-amino-3-hydroxy-5-methylisoxazole-4-proprionic acid HBr/kainate (AMPA/KA) antagonist, completely blocked hyperalgesia. (biopsychiatry.com)
When injected intrathecally (i.t.), kainic acid itself failed to induce hyperalgesia and AMPA/KA antagonists given i.t. also failed to attenuate the hyperalgesic effect of kainic acid administered i.p., indicating that the spinal cord is not the primary site of action. (biopsychiatry.com)
Glutamate receptor2
The glutamate receptor agonist kainic acid [21] and the cholinergic agonist pilocarpine [22] are commonly used in SE models. (jle.com)
Intrastriatal injections of kainic acid, an agonist of a subtype of glutamate receptor, produce lesions similar to those seen in HD. (medscape.com)
Rats7
The dose, rate and technique to administer kainic acid were explained extensively to reflect a successful paraplegia and spinal cord injury in rats. (afpm.org.my)
CONCLUSIONS: Kainic acid intra-spinal injection at the concentration of 0.05 mM, and rate 10 µL/min, is an effective method create spinal injury in rats, however more potent concentrations of kainic acid need to be studied in order to create severe spinal injuries. (afpm.org.my)
Intraperitoneal (i.p.) injection of kainic acid induced a persistent thermal hyperalgesia, when tested using the hot plate (mice) and tail flick (mice and rats) assays, and mechanical hyperalgesia when tested using von Frey monofilaments (rats), but had no effect on acetic acid-induced chemical nociception (mice). (biopsychiatry.com)
Because of the structural analogy between GLA and glutamate, GLA-exposed rats were examined for the wet-dog shakes response to kainic acid. (go.jp)
The frequency of wet-dog shakes induced by kainic acid showed a significant decrease in all GLA-exposed rats, though there was no significant difference among three dose groups. (go.jp)
The effects of DMF in preventing the onset of epilepsy and modifying the disease were investigated in the kainic acid-induced status epilepticus model of temporal lobe epilepsy in rats. (biomedcentral.com)
We generated GABAergic interneuron precursors from human embryonic stem cells (hESCs) and grafted them in the hippocampi of rats developing chronic SRSs after kainic acid‐induced status epilepticus. (lu.se)
Excitatory2
Kainic acid (KA) is well-known as an excitatory, neurotoxic substance. (koreamed.org)
Excitatory Amino Acids and Synaptic Plasticity. (tocris.com)
Neurons2
Thus, in large, concentrated doses kainic acid can be considered a neurotoxin, and in small doses of dilute solution kainic acid will chemically stimulate neurons. (wikipedia.org)
Intrastriatal injections of quinolinic acid, an N -methyl-D-aspartate (NMDA) receptor agonist, selectively affect medium-sized GABA-ergic spiny projection neurons, sparing the striatal interneurons and closely mimicking the neuropathology seen in HD. (medscape.com)
Neuronal cell2
Kainic acid is utilised in primary neuronal cell cultures and in the acute brain slice preparation to study the physiological effect of excitotoxicity and assess the neuroprotective capabilities of potential therapeutics. (wikipedia.org)
Prolonged (48 h) but not transient (4 h) kainic acid treatment caused regionally selective neuronal cell death. (duke.edu)
Injection2
Therefore, the most effective ablation studies are performed in comparison to a sham lesion that duplicates all the steps of producing a brain lesion except the one that actually causes the brain damage, that is, injection of kainic acid or administration of an electrical shock. (wikipedia.org)
Kainic acid injected subcutaneously in the back of mice decreased response latencies in the hot plate and tail flick assays, indicating that hyperalgesia is achieved by a variety of parenteral routes of injection. (biopsychiatry.com)
Kainate1
Kainic acid, or kainate, is an acid that naturally occurs in some seaweed. (wikipedia.org)
Amino Acids3
In the course of study, every non-polar amino acid in the model is replaced by glycine, whereupon the biochemical characteristics of polar amino acids should become more distinguished. (go.jp)
The DEV analyses of the models select four amino acids ( H, D, S and C ) as the essential members of the functional system. (go.jp)
The first three amino acids are coincide with the active sites of trypsin (40- H , 84- D and 177- S ). When the area of triangle made up of a combination of three amino acids out of the above four is measured, the triangle of HDS is found to be the minimum among those made of other combinations investigated, suggesting that they are closely located each other. (go.jp)
Temporal1
Ameliorative influence of cnestis ferruginea vahl ex DC (connaraceae) root extract on kainic acid-induced temporal lobe epilepsy in mice: Role of oxidative stress and neuroinflammation. (iasp-pain.org)
Epilepsy1
On the contrary, knockdown of Sprouty proteins increases proliferation of activated astrocytes and, consequently, reduces secondary brain damage in neuronal lesion models such as kainic acid-induced epilepsy or endothelin-induced ischemia. (springer.com)
Binds1
Okadaic acid binds to intestinal epithelial cells and increases their permeability. (medscape.com)
Neurodegenerative3
Histological evaluation of rat spinal cord and dorsal root ganglia revealed no neurodegenerative changes 24 h after kainic acid. (biopsychiatry.com)
The neuroprotective effects of berry fruits on neurodegenerative diseases are related to phytochemicals such as anthocyanin, caffeic acid, catechin, quercetin, kaempferol and tannin. (lww.com)
Data from in vitro and animal studies suggest that among the sources of antioxidants, phytochemicals in berry fruits ( e.g. , anthocyanin and caffeic acid) have a beneficial role in brain aging and neurodegenerative disorders because of their anti-oxidative, anti-inflammatory, anti-viral and anti-proliferative properties (Youdim et al. (lww.com)
Excitotoxicity1
The kainic acid (KA) excitotoxicity model is a convenient, low-cost, and highly reproducible animal model of SCI in the laboratory. (afpm.org.my)
Neurotoxin1
It is an analogue of kainic acid and a neurotoxin which causes amnesic shellfish poisoning (ASP). (mcw.edu)
Spinal cord1
In this protocol, detailed description of a dorsal laminectomy was explained to expose the spinal cord, following intra-spinal kainic acid administration at desired location. (afpm.org.my)
Axons1
Direct visualization of mossy fiber axons with neurobiotin-labeling revealed that mossy fibers in kainic acid-treated cultures exhibited a dramatic increase in supragranular axonal branch points and synaptic boutons. (duke.edu)
Citation1
Transplantation of cells releasing γ‐aminobutyric acid (GABA) could be used to counteract the imbalance between ex-citation and inhibition within epileptic neuronal networks. (lu.se)
Toxins1
The toxins responsible for most shellfish poisonings are water-soluble, are heat and acid-stable, and are not inactivated by ordinary cooking methods. (medscape.com)
Mice1
Mice lacking miR-22 displayed normal behaviour and brain structure and developed similar status epilepticus after intraamygdala kainic acid compared to wildtype animals. (researchsquare.com)
Commonly1
Kainic acid is commonly injected into laboratory animal models to study the effects of experimental ablation. (wikipedia.org)
Significantly1
Supply shortages beginning in 2000 have caused the cost of kainic acid to rise significantly. (wikipedia.org)
Cost1
The basic impactor approximately cost between 10,000 and 20,000 USD, while the kainic acid only cost between 300 and 500 USD, which is quite cheap as compared to traditional SCI method. (afpm.org.my)
Results1
The results suggest that exposure to GLA, even in low doses, during infantile period in the rat induces alterations in the kainic acid receptor in the brain. (go.jp)
Period1
Cultures treated with kainic acid for a prolonged period displayed a time- and dose-dependent increase in supragranular Timm staining reflective of increased mossy fiber innervation to this area. (duke.edu)
Chemical1
Therefore, chemical stimulation by kainic acid is more localized than electrical stimulation. (wikipedia.org)
Form1
The first step of the pathway involves the N-prenyltransferase, KabA, which allows for the prenylation of L-glutamic acid with dimethylallyl pyrophosphate (DMAPP) to form the intermediate N-dimethylallyl-l-glutamic acid (prekainic acid). (wikipedia.org)
Response1
They were weaned at 3 weeks of age and tested for the response to 9mg/kg kainic acid at 5 or 6 weeks of age. (go.jp)