Monoclonal antibodies were raised against antibodies to distinct carboxy-terminal KDEL sequences of two soluble, resident endoplasmic reticulumproteins. (ox.ac.uk)
The presence of carboxy terminal KDEL appears to be necessary forretention and appears to be sufficient to reduce the secretion of proteins from the ER. (exposed-skin-care.net)
Peptide6
KDEL is a target peptide sequence in mammals and plants located on the C-terminal end of the amino acid structure of a protein. (wikipedia.org)
11. Chimerical peptides according to the claim 1, characterized by containing a cell penetrating segment which is one of the following cationic PTDs: TAT peptide, heptaarginine, octaarginine, nonaarginine, decaarginine, or a peptide of sequence LRRRRLRRRRL or LRRRRL-bAla-LRRRRL. (patentsencyclopedia.com)
[6] The first 5-30 amino acids polymerized encode a signal peptide , a molecular message that is recognized and bound by a signal recognition particle (SRP). (ipfs.io)
However, their heterologous expression in a practical and efficient manner demands some structural requirements such as a minimum size, the incorporation of retention signals to assure their accumulation in specific tissues, and the presence of protease cleavage amino acids and of target sequences to facilitate peptide detection. (udg.edu)
Provided herein are anti-angiogenic comprising the N-terminal end of endostatin, nucleic acids encoding the same, pharmaceutical preparations comprising an effective amount of the peptide and nucleic acids and use of the pharmaceuticals in treating or preventing diseases or conditions associated with undesirable angiogenesis. (justia.com)
The so-called RxLR effectors share a number of structural features and have modular architectures: an N-terminal signal peptide, followed by the RxLR motif and then often (but not always) associated with a downstream dEER sequence in a disordered region. (eu.org)
Endoplasmic reticulum4
The KDEL sequence prevents a protein from being secreted from the endoplasmic reticulum (ER) and facilitates its return if it is accidentally exported. (wikipedia.org)
Moreover, since the presence of a KDEL unit or of tag54 did not influence significantly the biological activity, these moieties can be introduced when designing compounds to be retained in the endoplasmic reticulum and detected using a complementary epitope. (udg.edu)
The mAb fusion to the KDEL signal sequence helped to retain the protein inside the endoplasmic reticulum (ER), thus enhancing mAb assembly in plant cells (17). (researchensemble.com)
HDEL3
The similar sequence HDEL performs the same function in yeasts, while plants are known to utilize both KDEL and HDEL signaling sequences. (wikipedia.org)
The abbreviation HDEL follows the same notation as KDEL: H-Histidine D-Aspartic acid E-Glutamic acid L-Leucine Three letter code is: His-Asp-Glu-Leu. (wikipedia.org)
Proteins that stay in the ER, so-called ER resident proteins, briefly leave the ER via COPII, but are recognized by the recycling receptor (ERD2) which binds to a terminal four amino acid sequence (KDEL or HDEL). (oup.com)
Receptor4
KDEL receptor binding is dependent on pH, in which the ligand (target protein) binds strongly to the receptor in the cis-Golgi due to the unique low pH (6, in in vitro experiments pH 5 shows strongest binding) characteristic of the biochemical environment of the cis-Golgi network. (wikipedia.org)
As the vesicle that contains the KDEL receptor reaches the ER, the receptor is inactive due to the high pH (7.2-7.4) of the ER, resulting in the release of the target protein/ligand. (wikipedia.org)
demonstrating the dynamics of the KDEL receptor clustering's time dependence with a full experiment from start to finish (60 minutes). (wikipedia.org)
Thisretention is reported to be mediated by a KDEL receptor. (exposed-skin-care.net)
Soluble2
The soluble resident protein will remain in the ER as long as it contains a KDEL signal sequence on the C-terminal end of the protein. (wikipedia.org)
Here we present a new approach to incorporate single or multiple post-translational modifications or non-canonical amino acids into soluble and membrane proteins expressed in eukaryotic cells. (biorxiv.org)
Protein9
A protein with a functional KDEL motif will be retrieved from the Golgi apparatus by retrograde transport to the ER lumen. (wikipedia.org)
6. Chimerical peptides according to the claim 1, characterized by containing a segment inhibitor of the activation of the NS3pro protease which comprise the region Asp50-Glu62 of the NS2B protein from DV2 or the structurally equivalent segment of the protein NS2B from other Flavivirus, or an amino acid sequence showing a sequence similarity higher of equal to the 80% identity. (patentsencyclopedia.com)
8. Chimerical peptides according to the claim 1, characterized by containing a segment inhibitor of the activation of the NS3pro protease which comprise the region Thr20-Leu31 of the NS4A protein from HCV or the structurally equivalent segment of the protein NS4A from other Hepacivirus, or an amino acid sequence showing a sequence similarity higher of equal to the 80% identity. (patentsencyclopedia.com)
Sequence analysis showed that the complete open reading frame (ORF) of TaABP1-D was 1887 bp in length, encoding a putative protein composed of 205 amino acids with theendoplasmic reticulum retention sequence Lys-Asp-Glu-Leu (KDEL) and box regions. (chinacrops.org)
Sequence alignment proved that TaABP1-D shared high homology with rice OsABP1 (82% protein sequence identity) and other plant ABPs reported. (chinacrops.org)
A ribosome only binds to the RER once a specific protein-nucleic acid complex forms in the cytosol. (ipfs.io)
We identified a lipA gene in the genome sequence of Acremonium alcalophilum , encoding a protein with a predicted lipase domain with weak sequence identity to characterized enzymes. (biomedcentral.com)
The function of a protein is determined by the number and sequence of amino acids, which controls the three-dimensional structure of the resulting folded polypeptide. (frontiersin.org)
GRP78 is a highly conserved protein that is essential for cell viability.The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C terminus of GRP78and other resident ER proteins including glucose regulated protein 94 (GRP 94) and proteindisulfide isomerase (PDI). (exposed-skin-care.net)
Proteins4
Proteins can only leave the ER after this sequence has been cleaved off. (wikipedia.org)
The transportation mechanism of proteins containing the KDEL signal sequence is facilitated by KDEL receptors attached to COPII and COPI vesicles. (wikipedia.org)
KDEL receptors initiate the mechanism by which proteins are transported from the Golgi to the ER. (wikipedia.org)
The conserved Arg-X-Leu-Arg (RxLR) sequence motif was shown to play an essential role in transporting the secreted pathogen proteins across the plant membrane ( Dou,2008 ). (eu.org)
Vesicle1
BSMV infection of transgenic plants tagged with GFP-KDEL exhibited membrane proliferation and vesicle formation that were especially evident around the nucleus. (ppjonline.org)
Flavivirus1
Screening test for neutralizing antibodies against yellow fever virus, based on a We analyzed the whole-genome sequence of African swine flavivirus pseudotype. (cdc.gov)
Motif2
Considering the first part of the RxLR dEER dipartite motif, in oomycetes the 3rd and 4th positions seem to be the most conserved (Leu-Arg), while some level of amino acid substitution is allowed at the 2nd position (typically Phe-Gln-Ser-Leu). (eu.org)
In AVR3a ( D0NPN8 ) the acetylation occurs right after the RxLR motif is cleaved at Lys-48, the first amino acid downstream of the RxLR( Wawra,2017 ). (eu.org)
Fragment3
We prepared 40 analogues obtained by incorporating repeated units of the antimicrobial undecapeptide, fragments of natural peptides, one or two AGPA hinges, a Gly or Ser residue at the N-terminus, and a KDEL fragment and/or the epitope tag54 at the C-terminus. (udg.edu)
Best sequences contained a magainin fragment linked to the antimicrobial undecapeptide through an AGPA hinge. (udg.edu)
Endostatin, a 183 amino acid proteolytic cleavage fragment corresponding to the C-terminus of collagen 18, has been the subject of investigation by a number of laboratories because of its anti-tumor activity with no toxic side effects (O'Reilly et al. (justia.com)
Peptides3
Which of the P4Hs act on signaling peptides, and whether or not they differ in preference for proline in a certain sequence context is still unclear. (elifesciences.org)
Since any sequence modification may influence the biological activity, peptides that will be obtained from the expression must be screened prior to the synthesis of the genes for plant transformation. (udg.edu)
Based on these findings, the disclosure features anti-angiogenic peptides comprising at least about 12 amino acids of SEQ ID Nos. (justia.com)
Receptors2
The KDEL signal sequence is recognized by KDEL receptors, which are commonly located in the cis-Golgi, lysosomes, and secretory vesicles. (wikipedia.org)
demonstrated through experimentation and simulation that KDEL receptors/cargo clustering at the cell surface is caused by the transport of cargo-synchronized receptors from and to the plasma membrane. (wikipedia.org)
Identity1
Unusually, the predicted lipase displays ≈ 30% amino acid sequence identity to both feruloyl esterase and lipase of Aspergillus niger . (biomedcentral.com)
Gene1
In this study, the genomic sequence of a novel gene, designated TaABP1 , isolated from the hexaploid wheat ( Triticum aestivum L.) Chinese Spring genome databases. (chinacrops.org)
Presence1
The critical requirement for splicing to occur is typically the presence of a Cys, Ser or Thr side chain (depending on the split intein in question) in the +1 position of the extein (the sequence flanking the split intein) and multiple split inteins have recently been optimized for increased splicing efficiency 10 - 12 . (biorxiv.org)
Analysis1
Thus, the rebound could be attributed to host immune response to yellow fever virus Comparative Analysis of rather than to another pathogen or to direct effects of the vi- Whole-Genome Sequence of rus itself. (cdc.gov)
Domains1
The rod domain is flanked by globular head (amino) and tail (carboxyl) domains the functions of which are still unclear. (exposed-skin-care.net)
Nucleotide4
We analyzed the resulting sequence data by mapping against an improved ASFV Georgia 2007/1 sequence (International Nucleotide Sequence Database Collaboration accession no. (cdc.gov)
The whole-genome sequence is available from the International Nucleotide Sequence Database Collaboration databases under study accession no. (cdc.gov)
Comparison with the improved ASFV Georgia 2007/1 sequence revealed 15 differences, for an overall sequence identity of 99.98% at the nucleotide level. (cdc.gov)
The complete nucleotide sequences of 11 isolates have been determined. (ictv.global)
Mammalian1
Three predicted N -glycosylation consensus sequences on EOGT are highly conserved among mammalian species. (nih.gov)
Enzyme2
An enzyme that, in the course of purine ribonucleotide biosynthesis, catalyzes the conversion of 5'-phosphoribosyl-4-(N-succinocarboxamide)-5-aminoimidazole to 5'-phosphoribosyl-4-carboxamide-5-aminoimidazole and the conversion of adenylosuccinic acid to AMP. (lookformedical.com)
The recombinant plasmid was constructed and identified by PCR, enzyme digestion and sequencing. (bvsalud.org)
Nucleic4
A ribosome only binds to the RER once a specific protein-nucleic acid complex forms in the cytosol. (ipfs.io)
Since the isolation of nucleic acids to the mapping of human genome, chemical synthesis of nucleic acids has undergone tremendous advancements. (researchgate.net)
Further improvements in this area such as, introduction of high throughput synthesizers, better coupling reagents, improved polymer supports, newer sets of protecting groups for exocyclic amino groups of nucleic bases and introduction of universal polymer supports have completely revolutionized the entire field of nucleic acids chemistry. (researchgate.net)
Microarray technology has become an important tool for detection and analysis of nucleic acid targets. (researchgate.net)
Genome5
Thus, the rebound could be attributed to host immune response to yellow fever virus Comparative Analysis of rather than to another pathogen or to direct effects of the vi- Whole-Genome Sequence of rus itself. (cdc.gov)
Screening test for neutralizing antibodies against yellow fever virus, based on a We analyzed the whole-genome sequence of African swine flavivirus pseudotype. (cdc.gov)
We aligned different available ASFV whole-genome sequences using MAFFT 7.388, and we performed a phylogenetic analysis using IQ-TREE v1.6.5 ( 8 , 9 ). (cdc.gov)
The ASFV Belgium 2018/1 whole-genome sequence has a length of 190,599 bp. (cdc.gov)
PCR Closing gaps in the assembly PCR with primers targeting particular areas on the two JP environmental genomes have been employed to confirm the genome assembly, raise sequencing coverage and reconfirm the presence of notable genome characteristics. (tcr-signal.com)
Partial2
Partial sequencing at the EURL revealed a p72 genotype II with CVR-1, IGR-2, and MGF1 variants. (cdc.gov)
GTP, gap and article), 3) organization of the catabolism upon Following of the such back-to-back acid, and components production, germ and dissociation, with partial X to the severe XIAP, 4) physiological resulting and cycle protein microRNA, and 5) GTP haem and allowing of the tissue-specific new metalloproteinase. (evakoch.com)
Codons1
The gene sequence of AMP BSN-37 was obtained by codons reverse translation, and the codons were optimized according to the codons preference of Escherichia coli (E. coli). (bvsalud.org)
Signal4
The soluble resident protein will remain in the ER as long as it contains a KDEL signal sequence on the C-terminal end of the protein. (wikipedia.org)
[6] The first 5-30 amino acids polymerized encode a signal peptide , a molecular message that is recognized and bound by a signal recognition particle (SRP). (ipfs.io)
A) The organization of TMTC3 and TMTC4 with signal sequences (black), hydrophobic domains (teal), and TPR domains (orange) as designated. (xenbase.org)
What effect would be the addition of a lysosomal signal sequence have of subcellular localization of a protein that is normally cytosolic? (wizedu.com)
20181
For the assembly of the inverted terminal repeat (ITR) regions, we mapped the reads against the individual ASFV-Georgia 2007/1 ITR regions using Newbler and manually assembled them with the ASFV Belgium 2018/1 sequence in Geneious 11.1.5 (Biomatters, https://www.geneious.com ). (cdc.gov)
Target2
KDEL is a target peptide sequence in mammals and plants located on the C-terminal end of the amino acid structure of a protein. (wikipedia.org)
In addition, we enriched DNA libraries for Illumina sequencing for ASFV specific target sequences using an ASFV-specific myBaits kit (Arbor Biosciences, https://arborbiosci.com ). (cdc.gov)
Cells1
Cell Culture and Hypoxia Treatment MCF-7 cells (DSMZ ACC 115) were produced in DMEM supplemented with heat-inactivated FBS (10%) nonessential amino acids penicillin and streptomycin. (tam-receptor.com)
Long1
The end sequences are present as two flip-flop forms that are inverted and complementary with respect to each other, and adjacent to both termini are identical tandem repeat arrays about 2.1 kbp long. (ictv.global)
Similar1
The similar sequence HDEL performs the same function in yeasts, while plants are known to utilize both KDEL and HDEL signaling sequences. (wikipedia.org)
African1
The high-coverage sequence revealed 15 differences compared with an improved African swine fever virus Georgia 2007/1 sequence. (cdc.gov)
Important1
This reaction represents an important step in fatty acid biosynthesis. (lookformedical.com)