• Jurkat cells are an immortalized line of human T lymphocyte cells that are used to study acute T cell leukemia, T cell signaling, and the expression of various chemokine receptors susceptible to viral entry, particularly HIV. (wikipedia.org)
  • Jurkat cells can produce interleukin 2, and are used in research involving the susceptibility of cancers to drugs and radiation. (wikipedia.org)
  • Jurkat J6 cells have been found to produce a xenotropic murine leukemia virus (X-MLV) (referred to as XMRV) that could potentially affect experimental outcomes. (wikipedia.org)
  • Unlike wild type Jurkat cells, which express multiple HLA subtypes, the Jurkat HLA panel cell lines express individual MHC Class I HLA subtypes. (sigmaaldrich.com)
  • Our study shows that Jurkat human T cells have a molecule that reacts with both anti-CR2 antibodies and the third component of complement, C3. (nih.gov)
  • In addition, EBV variably infects Jurkat cells as demonstrated by the presence of transcripts of Epstein Barr nuclear Ag (EBNA-1) using the polymerase chain reaction. (nih.gov)
  • We previously described the accumulation of extracellular ATP /AMP during chemotherapy-induced apoptosis in Jurkat human leukemia cells. (aspetjournals.org)
  • In this study, we compared how different signaling pathways determine extracellular nucleotide pools in control Jurkat cells versus Jurkat lines that lack the Fas-associated death domain (FADD) or receptor-interacting protein kinase 1 (RIP1) cell death regulatory proteins. (aspetjournals.org)
  • Accumulation of extracellular ATP/AMP was similarly absent in RIP1-deficient Jurkat cells during apoptotic responses to chemotherapeutic agents. (aspetjournals.org)
  • The effect of incubation with 18:3ω-3 compared to 24:6ω-3 on the increment in 22:6ω-3 was tested in Jurkat cells because primary T cells cannot convert 18:3ω-3 to 22:6ω-3. (soton.ac.uk)
  • The increment in the 22:6ω-3 content of Jurkat cells incubated with 24:6ω-3 was 19.5-fold greater than that of cells incubated with 18:3ω-3. (soton.ac.uk)
  • Acyl-coA oxidase siRNA knockdown decreased the amount of 22:6ω-3 and increased the amount of 24:6ω-3 in Jurkat cells. (soton.ac.uk)
  • These findings show exogenous 24:6ω-3 can be incorporated into primary human T lymphocytes and Jurkat cells and induces changes in fatty acid composition consistent with its conversion to 22:6ω-3 via a mechanism involving peroxisomal β-oxidation that is regulated independently from the integrity of the upstream PUFA synthesis pathway. (soton.ac.uk)
  • By using Jurkat T cells, we now show how diphenhydramine inhibits Hv1 mediated currents, inducing a drop in intracellular pH and cellular viability. (unlp.edu.ar)
  • Inability of 50 Hz magnetic fields to regulate PKC- and Ca(2+)-dependent gene expression in Jurkat cells med. (emf-portal.org)
  • In this preliminary study we assessed the role of Protein kinase C (PKC) in CD47-mediated phosphatidylserine expression pathway in jurkat T cells. (scholarsresearchlibrary.com)
  • Jurkat T cells were incubated with anti-CD47 mAb (BRIC 126), anti-CD44 mAb (BRIC 235) or control in the presence or absence of Bisindolylmaleimide I, hydrochloride (PKC inhibitor). (scholarsresearchlibrary.com)
  • This indicates that PKC may be involved in CD47-mediated PS exposure pathway in jurkat T cells. (scholarsresearchlibrary.com)
  • The observations from this study identify CD47 and PKC as novel functional proteins in jurkat T cells with promising therapeutic potential. (scholarsresearchlibrary.com)
  • SDF-1α significantly induced the activation of LIMK1 in Jurkat human leukemic T cells and peripheral blood lymphocytes. (neuroprobe.com)
  • Treatment of Jurkat cells with S3 peptide inhibited the SDF-1α-induced cofilin phosphorylation, actin reorganization, and chemotactic response of Jurkat cells. (neuroprobe.com)
  • Enrichment of Jurkat cells having 10.1 average diameter in phosphate buffer solution (PBS) medium is achieved. (itu.edu.tr)
  • We irradiated Jurkat cells by using modulated beams of accelerated protons with energies up to 62 MeV, under a dose of 10 Gy distributed uniformly inside the cell suspension. (unict.it)
  • The treatments using menadione, hydrogen peroxide and quercetin as oxidant agents potently induced apoptosis of Jurkat cells in a dose-dependent manner and consistently decreasedthe intensity of delayed photoemission. (unict.it)
  • We obtained astrong anti-correlation between apoptosis of humanleukemia Jurkat cells and UV-induced delayed photoemission on a specific time interval ranging from100 μs to 1 ms after UV-excitation of the cell samples. (unict.it)
  • This study examined the post-thaw recovery of Jurkat cells cryopreserved in single osmolyte solutions containing sucrose, glycerol or isoleucine, as well as in a combination of the three osmolytes. (nature.com)
  • Post-thaw recovery of Jurkat cells in single osmolyte was low. (nature.com)
  • These studies demonstrated that a combination of three different osmolytes including sugar, sugar alcohol and amino acids/proteins could stabilize Jurkat cells and mesenchymal stromal cells (MSCs) during freezing. (nature.com)
  • By comparison, Jurkat cells have been used only scantily for imaging on supported lipid bilayers (SLBs) incorporating laterally mobile TCR and integrin ligands, which allow to study synaptic rearrangements of surface molecules and the fine architecture of the mature IS, likely due to limitations in the assembly of immune synapses with well-defined architecture. (ox.ac.uk)
  • Here we have explored whether upregulating the low levels of endogenous LFA-1 expression on Jurkat E6.1 cells through transduction with CD11a- and CD18-encoding lentiviruses can improve IS architecture. (ox.ac.uk)
  • For demonstration of our method, this chapter details the detection of miR155 and CD69 protein in PMA and ionomycin-stimulated Jurkat cells. (nih.gov)
  • In the present study, the anticancer activity of pleurocidin-like peptide WF3 isoform X2 (AMP-WF3), from the Poecilia Mexicana fish, against leukemic cell line Jurkat was evaluated, and the cytotoxicity compared with the effects on normal cells, including peripheral blood mononuclear cells (PBMCs) and human dermal fibroblast (HDF) cells. (celljournal.org)
  • Flow cytometry verified that the apoptotic activity of AMP-WF3 on Jurkat cells was significantly higher than that of HDF and PBMCs. (celljournal.org)
  • Peptide-treated Jurkat cells were associated with increased expression of P21, and P53 genes. (celljournal.org)
  • G, activated and Jurkat T cells(Sup. (icbinhibitor.com)
  • From the adjusted Q values we determined that the average prices of total Ca 2+ accumulation per cell will be 80 amolmin-1cell-1, 260 amolmin-1cell-1 and 350 amolmin-1cell-1, in resting, activated and Jurkat T cells, respectively. (icbinhibitor.com)
  • Despite the fact that this can be a rough estimate provided that quite a few parameters applied for this calculation are uncertain, it indicates that the average rate of [Ca 2+]i rise in resting T cells needs to be 2-fold higher than that in activated or Jurkat T cells. (icbinhibitor.com)
  • The purpose of this study was to determine if PGRP-S has a direct role in Fn induced apoptosis in Jurkat T cells. (umich.edu)
  • Conclusion: The results indicate that PGRP-S has a direct role in Fusobacterium nucleatum 's ability to induce apoptotic cell death in Jurkat T cells. (umich.edu)
  • DNA standards and GAPDH-2 primers were used to analyze DNA from Fixed Jurkat Cell Pellet samples (equivalent to 100,000 Jurkat cells). (activemotif.com)
  • Flow Cyt (intra): HeLa and Jurkat cells. (abcam.com)
  • To investigate whether carbamate pesticides also induce apoptosis in human T lymphocytes, in the present study Jurkat human T cells were treated in vitro with thiram, maneb, carbaryl or ziram. (researchgate.net)
  • Lysate from Jurkat cells was probed with anti-Fli-1 (clone G146-254) at concentrations of 2.0 (lane 1), 1.0 (lane 2), and 0.5 µg/ml (lane 3). (bdbiosciences.com)
  • Herein we report that DCPA attenuates the normal elevated and sustained [Ca2+](i) that follows internal store depletion in the human leukemic Jurkat T cell line and primary mouse T cells. (cdc.gov)
  • Exposure of Jurkat cells to either DCPA or 50 mu M 2-APB attenuated the increase in [Ca2+](i) following thapsigargin or anti-CD3 induced store depletion in a similar manner. (cdc.gov)
  • METHODS: Cell Counting Kit-8 (CCK-8) assay was used to detect the effect of methionine restriction on HL-60 and Jurkat cells proliferation. (bvsalud.org)
  • The effect of methionine restriction on cell cycle of HL-60 and Jurkat cells was examined by PI staining. (bvsalud.org)
  • Annexin V-FITC / PI double staining was applied to detect apoptosis of HL-60 and Jurkat cells following methionine restriction. (bvsalud.org)
  • CONCLUSION: Acute leukemia cells HL-60 and Jurkat exhibit methionine dependence. (bvsalud.org)
  • Methionine restriction can significantly inhibit the proliferation, promote cell cycle arrest and induce apoptosis of HL-60 and Jurkat cells, which suggests that methionine restriction may be a potential therapeutic strategy for acute leukemia. (bvsalud.org)
  • The Jurkat cell line (originally called JM) was established in the mid-1970s from the peripheral blood of a 14-year-old boy with T cell leukemia. (wikipedia.org)
  • This study compared the effect of incubation with 24:6ω-3 on the fatty acid composition of two related cell types, primary CD3+ T lymphocytes and Jurkat T cell leukemia, which differ in the integrity of the polyunsaturated fatty acid (PUFA) biosynthesis pathway. (soton.ac.uk)
  • We investigated the relation between apoptosis anddelayed luminescence (DL) in human leukemia Jurkat Tcellsundergoing various treatments. (unict.it)
  • Apoptotic activation triggered equivalent proteolytic gating of Panx1 channels in all three Jurkat cell lines. (aspetjournals.org)
  • The Jurkat E6.1 clone has been extensively used as a powerful tool for the genetic and biochemical dissection of the TCR signaling pathway. (ox.ac.uk)
  • Analysis of Jurkat and Raji mRNA by PCR demonstrated the presence of this novel splice variant in both cell lines. (nih.gov)
  • SMEZ expression was also analyzed by using the more sensitive Jurkat cell assay, which has a threshold of approximately 10 pg/mL. (cdc.gov)
  • c Jurkat assay. (cdc.gov)
  • After co-culturing Fn with Jurkats overnight with monoclonal antibodies to PGRP-S, the levels of Jurkat apoptosis was assessed via caspase assay according to manufacturer instructions (Promega Caspase-Glo 3/7 Assay Kit). (umich.edu)
  • For this purpose, we first compared the ability of G and N proteins of a pathogenic and a nonpathogenic strain to trigger apoptosis of Jurkat rtTA by using an inducible Tet-on expression system. (pasteur.fr)
  • Different derivatives of the Jurkat cell line that have been mutated to lack certain genes can now be obtained from cell culture banks. (wikipedia.org)
  • There was a significant dose dependent decrease in Jurkat-cell apoptosis upon co-culture with Fn in the presence of monoclonal anti-PGRP-S antibodies. (umich.edu)
  • Positive control samples (Jurkat cell pellets) and antibody ( AbFlex ® H3K9ac ) are included in the module for user optimization of the Low Cell ChIP protocol. (activemotif.com)
  • Northern blot analysis of Jurkat poly(A)+ RNA shows a transcript of 4.7 kb upon probing with the B cell CR2 cDNA. (nih.gov)
  • This product is a panel of eleven (11) genetically modified cell lines targeting MHC Class I HLA molecules in a Jurkat T lymphocyte parental cell line background. (sigmaaldrich.com)
  • The objective of this investigation is to understand in more detail the relationships amongst the osmolytes present in these solutions and Jurkat cell recovery. (nature.com)
  • Assuming that 85 with the total cell volume is occupied by the cytosol and nucleus,32,33 and that buffering capacity of the cytosol is one hundred,33,34 we estimated that prices of [Ca 2+]i rise throughout Ca 2+ entry by way of maximally activated CRAC channels were 110 nM/s, 57 nM/s and 65 nM/s in resting, activated and Jurkat T cell, respectively. (icbinhibitor.com)
  • Human platelet and Jurkat lysates. (abcam.com)
  • The NIP element forms four specific DNA -protein complexes (designated as complexes A-D) with the Jurkat nuclear proteins. (cdc.gov)
  • Jurkat and their derivative cell strains are immortal human T lymphocytes. (oxinst.com)
  • Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human FcγRIIIa, low affinity (F158) variant and Fcγ chain. (cobioer.com)
  • SMEZ expression was also analyzed by using the more sensitive Jurkat cell assay, which has a threshold of approximately 10 pg/mL. (cdc.gov)
  • Jurkat cell lines can also be used in screening of different antiretroviral treatment strategies. (oxinst.com)
  • Background: The leukaemia-derived Jurkat E6.1 cell line has been used as a model T cell in the study of many aspects of T cell biology, most notably activation in response to T cell receptor (TCR) engagement. (ox.ac.uk)
  • Jurkat control lysate [50 µg (1 µg/µl) is provided as a Western blot positive control (Comp. (bdbiosciences.com)