Residues1999Genome2023Biological Chemistry1997Molecular biologyProteinsBiochemistryHttpsReceptorsProteinCleavage2022GenesMutagenEfficiencyMutations2017MechanismsMutationIdentificationArticleSequenceEnzymeStructuralCentersTransition2021HemeCancerConstituteClinicalActivityMethodCancersStudySpecificBiologyWork
Residues2
- In an attempt, first, to distinguish between two proposed structural models of this di-iron protein, and, second, to examine the roles of two highly conserved tyrosine residues, we have expressed an array of site-specific mutants inSchizosaccharomyces pombe. (figshare.com)
- Insight into the Function of Active Site Residues in the Catalytic Mechanism of Human Ferrochelatase. (uga.edu)
19991
- 80 (Pt 2), 291-296 (1999) PUBMED 10073687 REFERENCE 3 (bases 1 to 7654) AUTHORS Liu,B., Clarke,I.N. and Lambden,P.R. TITLE Polyprotein processing in Southampton virus: identification of 3C-like protease cleavage sites by in vitro mutagenesis JOURNAL J. Virol. (cdc.gov)
Genome5
- Since 2013, the development of the CRISPR/Cas9 technology, based on a prokaryotic viral defense system, has also allowed for the editing of the genome, and mutagenesis may be performed in vivo with relative ease. (wikipedia.org)
- 2016. Seamless and site-directed mutagenesis of the Saccharomycescerevisae genome using CRISPR-Cas9. (concordia.ca)
- The regenerated genome-edited events of Gonja Manjaya showed mutations in the targeted sites with the potential to prevent proper transcription or/and translational into functional viral proteins. (nature.com)
- Multiple copies of eBSV viral sequences have become integrated as direct and inverted tandem repeats at a single locus in the B genome of the host during viral infection 5 . (nature.com)
- The genome-edited events of plantain Gonja Manjaya were generated with mutations in the targeted sites of integrated eBSV sequences in the host genome. (nature.com)
20232
Biological Chemistry2
19971
- U. Ryde, L. Hemmingsen (1997) "The active-site metal coordination geometry of cadmium-substituted alcohol dehydrogenase. (lu.se)
Molecular biology2
- Site-directed mutagenesis is a molecular biology method that is used to make specific and intentional mutating changes to the DNA sequence of a gene and any gene products. (wikipedia.org)
- Journal of Molecular Biology 2008, 381 (2), 373-382. (ncl.ac.uk)
Proteins3
- In proteins where cysteine is not within the active site, activity can be modulated by changing conformation or by influencing its regulatory role, for example, iron sulfur complexes (ISCs) in aconitase possess cysteines required for its activity [ 7 ]. (hindawi.com)
- Allosterically regulated proteins that require an activator are sometimes based on a thiol exchange interaction involving cysteines, for example, pyruvate kinase uses fructose bisphosphate (FBPs) as a heterotrophic activator and it contains a cysteine in its FBP binding site [ 8 ]. (hindawi.com)
- Site-directed mutagenesis was used to selectively eliminate the IgG binding for each epitope and single and multiple mutations were expressed as recombinant GST fusion proteins. (cdc.gov)
Biochemistry1
- Routine biochemistry studies of catalytic mechanisms, for instance, routine studies using tools such as site-directed mutagenesis, are not typically suitable. (rsc.org)
Https1
- An animated Interactive 3D Complement (I3DC) is available in Proteopedia at https://proteopedia.org/w/Journal:IUCrJ:S2052252519012399 . (iucr.org)
Receptors1
- So far, the most successful passive immunotherapy directed to VEGF is Bevacizumab, a monoclonal antibody that neutralizes the binding of VEGF to its receptors [5], which has been approved, in combination with chemotherapy, for the treatment of different tumors [6-12]. (researchsquare.com)
Protein6
- Also called site-specific mutagenesis or oligonucleotide-directed mutagenesis, it is used for investigating the structure and biological activity of DNA, RNA, and protein molecules, and for protein engineering. (wikipedia.org)
- Cysteines often form part of active sites, allowing for the protein to be switched on or off depending on redox state. (hindawi.com)
- An Active Site Inhibitor Induces Conformational Penalties for ACE2 Recognition by the Spike Protein of SARS-CoV-2. (acs.org)
- Site-directed mutagenesis was utilised to map the domains involved in protein-protein interaction. (imperial.ac.uk)
- Expression of cloned envelope protein genes from the flavivirus tick-borne encephalitis virus in mammalian cells and random mutagenesis by PCR. (microbiologyresearch.org)
- Mutagenesis of the NS2B-NS3-mediated cleavage site in the flavivirus capsid protein demonstrates a requirement for coordinated processing. (microbiologyresearch.org)
Cleavage2
- Angiotensin-Converting Enzyme 2 Ectodomain Shedding Cleavage-Site Identification: Determinants and Constraints. (acs.org)
- 78 (9), 4827-4837 (2004) PUBMED 15078964 REFERENCE 2 (bases 1 to 7654) AUTHORS Liu,B.L., Viljoen,G.J., Clarke,I.N. and Lambden,P.R. TITLE Identification of further proteolytic cleavage sites in the Southampton calicivirus polyprotein by expression of the viral protease in E. coli JOURNAL J. Gen. Virol. (cdc.gov)
20221
- Journal of Chemical Information and Modeling 2022 , 62 (24) , 6574-6585. (acs.org)
Genes2
- A large number of methods are available to effect site-directed mutagenesis, although most of them have rarely been used in laboratories since the early 2000s, as newer techniques allow for simpler and easier ways of introducing site-specific mutation into genes. (wikipedia.org)
- Lotus Japonicus como Modelo para la Identificaci n de Nuevos Genes Implicados en la Respuesta a Estr s Abi tico y Productividad en Leguminosas. (us.es)
Mutagen1
- 310 "A high efficiency method for site-directed mutagen -esis with any plasmid":Gene;1989 (Dec 7);84(1);p153-7.Average yield of mutants was 60% with simple and rapid techniques. (globalchange.com)
Efficiency1
- Many approaches have since been developed to improve the efficiency of mutagenesis. (wikipedia.org)
Mutations3
- Site-directed mutagenesis is one of the most important laboratory techniques for creating DNA libraries by introducing mutations into DNA sequences. (wikipedia.org)
- Early attempts at mutagenesis using radiation or chemical mutagens were non-site-specific, generating random mutations. (wikipedia.org)
- Auxiliary active site mutations enhance the glycosynthase activity of a GH18 chitinase for polymerization of chitooligosaccharides. (iqs.edu)
20172
Mechanisms1
- Significant insight into their catalytic mechanisms has been obtained by relating site-directed mutagenesis studies to kinetic activity assays. (iucr.org)
Mutation5
- These methods of mutagenesis, however, are limited by the kind of mutation they can achieve, and they are not as specific as later site-directed mutagenesis methods. (wikipedia.org)
- This synthetic primer contains the desired mutation and is complementary to the template DNA around the mutation site so it can hybridize with the DNA in the gene of interest. (wikipedia.org)
- Here, a systematic approach to investigate the effect of an active-site-residue mutation on a model enzyme, human carbonic anhydrase II (CA II), is described. (iucr.org)
- Mutation of this sequence to the consensus Fnr-binding sequence by changing two bases in each half site caused nnrS expression to become nitrate independent. (microbiologyresearch.org)
- Somatic mutation is considered the primary direct cause of tumorigenesis. (hep.com.cn)
Identification1
- Identification by endogenous ADP-ribosylation and site-directed mutagenesis. (genome.jp)
Article1
- Journal Article Villalba, Miryam I. (osti.gov)
Sequence2
- This antigen sequence represents the complete Bevacizumab binding site and using RFASE as adjuvant, the vaccine (hVEGF 26-104 /RFASE) induces an immune response with VEGF neutralizing activity and anti-tumor effect [15]. (researchsquare.com)
- M sequence splice site analysis revealed an evolutionarily conserved single nucleotide variant C55T in H3N2, which impaired M2 expression and was accompanied by collinear M1 and mRNA3 production. (biomedcentral.com)
Enzyme4
- Enzyme active sites provide highly optimized microenvironments for their specific substrates by providing reactive groups such as nucleophiles or acids/bases that stabilize the transition state. (iucr.org)
- Each protomer contains a deep hydrophobic pocket which is proposed to constitute the active site of the enzyme. (iucr.org)
- Finally, combined quantum mechanical/molecular mechanical (QM/MM) modelling of the IdmH reaction shows that the active site of the enzyme provides an appropriate environment to promote indane-ring formation, supporting the assignment of IdmH as the key Diels-Alderase catalysing the final step in the biosynthesis of indanomycin through a similar mechanism to other recently characterized Diels-Alderases involved in polyketide-tailoring reactions. (iucr.org)
- Thiolate ligated heme enzyme spectroscopic determination of their active site structure and mechanistic implications of thiolate ligation. (microbiologyresearch.org)
Structural2
- Structural and biochemical characterization of two heme binding sites on α1-microglobulin using site directed mutagenesis and molecular simulation. (lu.se)
- Metal centers constitute the active sites of at least one third of all enzymes and determining the assembly mechanism of metallocenters and the electronic and structural properties of metal centers that confer selective and specific catalytic activity present fascinating challenges to inorganic chemists. (uga.edu)
Centers1
- The Centers for Disease Control and Prevention (CDC) cannot attest to the accuracy of a non-federal website. (cdc.gov)
Transition1
- Site-directed mutagenesis was achieved in 1974 in the laboratory of Charles Weissmann using a nucleotide analogue N4-hydroxycytidine, which induces transition of GC to AT. (wikipedia.org)
20212
Heme1
- A1M binds heme and the crystal structure suggests that C34 and H123 participate in a heme binding site. (lu.se)
Cancer4
- RÉSUMÉ Des études en milieu hospitalier ont révélé des fréquences relatives très élevées du cancer oropharyngé au Yémen. (who.int)
- La présente étude a estimé les fréquences relatives du cancer de la cavité buccale et du pharynx chez des patients yéménites atteints de cancers enregistrés en 2007 et 2008. (who.int)
- The primary site (topogra- oral cancer or pharyngeal cancer and of whom (273 000) died of the disease phy) and histology (morphology) of were excluded ( n = 110). (who.int)
- Worldwide those affecting the oral cavity, pharynx variations in oral cancer distribution or both sites. (who.int)
Constitute1
- Linking to a non-federal website does not constitute an endorsement by CDC or any of its employees of the sponsors or the information and products presented on the website. (cdc.gov)
Clinical1
- Journal of Clinical Microbiology. (edu.au)
Activity1
- UDS activity in the rat liver of the human carcinogens benzidine and 4-aminobiphenyl, and the rodent carcinogens 3,3'-dichlorobenzidine and direct black 38. (cdc.gov)
Method1
- Hutchison later produced with his collaborator Michael Smith in 1978 a more flexible approach to site-directed mutagenesis by using oligonucleotides in a primer extension method with DNA polymerase. (wikipedia.org)
Cancers3
- The tongue was the most affected oral site (53.6%) while the nasopharynx comprised 89.5% of pharyngeal cancers. (who.int)
- La langue était le site le plus souvent touché de la cavité buccale (53,6 %) alors que le nasopharynx était impliqué dans 89,5 % des cancers du pharynx. (who.int)
- Oral and pharyngeal cancers were es- there were discrepancies between ondary and unspecified sites (C76- timated to affect more than 482 000 data sources, the data in the hard files C80) were erroneously registered as people globally in 2008, more than half were used. (who.int)
Study1
- First site-directed mutagenesis study on the active-site of the plant alternative oxidase to support current structure. (figshare.com)
Specific1
- Glucosyltransferases synthesize exopolysaccharides, which are glucans that promote the accumulation of microorganisms at specific sites on dental surfaces, and glucosyltransferases become enzymatically active when exposed to dietary sucrose. (bvsalud.org)