• Mutant line R3-2 exhibited a nearly 1000-fold resistance to infection by genetically marked Moloney murine leukemia virus genomes, and was resistant to pseudotyped viruses utilizing the ecotropic envelope, the amphotropic envelope, or even the VSV G envelope protein. (biomedcentral.com)
  • The First cDNA strand was synthesized using antisense primer (SqMV-CPR) and Moloney murine leukemia virus (MMuLV) reverse transcriptase (Parstoos, Iran). (ac.ir)
  • TRIzol reagent, random primers and Moloney murine leukemia virus reverse transcriptase have been obtained from Invitrogen. (acthreceptor.com)
  • RNA isolation and qPCR Total cellular RNA was extracted from 3T3-L1 cells and mice epididymal fat pads utilizing TRIzol reagent as previously reported.50,51 The cDNA was synthesized from 1 g of total RNA in 20 L working with random primers and Moloney murine leukemia virus reverse transcriptase. (acthreceptor.com)
  • After chilling on ice, the next components had been put into the examples: 5 invert transcriptase buffer, 2.5 mM dNTPs, RNase inhibitor, and Moloney Murine Leukemia Virus invert transcriptase. (careersfromscience.org)
  • The ERGB/Fli-1 gene: isolation and characterization of a new member of the family of human ETS transcription factors. (musc.edu)
  • These DNAs constitute valuable tools in the characterization of this line's virus resistance. (biomedcentral.com)
  • Subsequent characterization of this virus has shown that it is not significantly related to any known animal retrovirus, is not an endogenous (genetically transmitted) virus of man, and so far has been associated only with fresh or cultured T -cells from patients with T -cell neoplasia. (science-connections.com)
  • Here, we describe the isolation and genomic characterization of six Egyptian (2015), four Pakistani (2015), and two Ukrainian (2007, 2013) recent pigeon-derived NDV isolates of sub-genotype VIg. (biomedcentral.com)
  • All of these viruses, and the ancestral Bulgarian ( n = 2) and South Korean ( n = 2) viruses described here, have predicted virulent cleavage sites of the fusion protein, and those selected for further characterization have intracerebral pathogenicity index assay values characteristic of NDV of genotype VI (1.31 to 1.48). (biomedcentral.com)
  • Characterization of murine pregnancy decidua transforming growth factor beta. (mcmaster.ca)
  • A novel gammaretrovirus named xenotropic murine leukemia virus-related virus (XMRV) has been recently identified and found to have a prevalence of 40% in prostate tumor samples from American patients carrying a homozygous R462Q mutation in the RNaseL gene. (biomedcentral.com)
  • In this virus, the gene for the LCP (41 kDa) and SCP (22 kDa) was mapped by in vitro translation studies to RNA-2 (contained within the M component), which is in concordance with established information on CPMV and the other comoviruses. (ac.ir)
  • The methods for gene isolation developed here will also permit the identification of similar suppressors that modify or override other recently identified virus resistance genes. (biomedcentral.com)
  • To identify such genes, we have developed methodologies that allow for the selection of rare virus-sensitive clones arising after transfer of gene libraries into populations of virus-resistant parents. (biomedcentral.com)
  • However, the validated rRT-PCR test that is normally used to identify the virulent fusion gene fails to detect the Egyptian and Ukrainian viruses due to mismatches in primers and probe. (biomedcentral.com)
  • The housekeeping gene hypoxanthine guanine phosphoribosyltransferase (HPRT) regulates multiple developmental and metabolic pathways of murine embryonic stem cell neuronal differentiation. (ucsd.edu)
  • Herpes simplex virus thymidine kinase (HSV-tk) is a well-established suicide gene that has been successfully used to control GvHD following donor lymphocyte infusions (DLI) after allogeneic stem cell transplantation. (haematologica.org)
  • Hepatitis E virus (HEV) is the causative agent of acute hepatitis E, which is endemic to many developing countries and occurs sporadically in some industrialized countries. (cdc.gov)
  • Similar expansions could also be obtained from the peripheral blood of patients with acute leukemia undergoing therapy ( n = 11). (aacrjournals.org)
  • The expanded NK cells were significantly more potent than unstimulated or IL-2-stimulated NK cells against acute myeloid leukemia cells in vitro . (aacrjournals.org)
  • 1 month when injected into immunodeficient mice and could eradicate leukemia in murine models of acute myeloid leukemia. (aacrjournals.org)
  • There are various clinical presentations of T -lymphocytic neoplasia in man, including approximately 25% of both childhood and adult acute lymphatic leukemia (Brouet et al. (science-connections.com)
  • While heading up the lab of Robert Gallo in 1977, Rusectti made scientific history by co-discovering with Bernie Poiesz, the first human retrovirus, HTLV-1 (human T-cell leukemia virus). (nexusnewsfeed.com)
  • Primers were designed for canine distemper virus nucleocapsid protein coding region fragment amplification (84 bp). (scielo.br)
  • The protocol involves the transformation of pools of resistant cells by cDNA expression libraries, followed by the selection for rare virus-sensitive cells, using multiple rounds of selection after infection by marked viral vector genomes. (biomedcentral.com)
  • Episomal simian virus 40 genomes in human brain tumors. (vaccineinjury.info)
  • 1975). Although the Epstein-Barr virus (EBV), a DNA virus of the herpes group, is implicated in some aspect of Burkitt's lymphoma, a B-cell disease (De- The 1980), the etiology of all the T -cell neoplasias is, as of yet, obscure. (science-connections.com)
  • When bovine leukemia virus (BLV), a causative agent of B-cellleukemia and lymphoma of cows, is injected into sheep, lymphoid leukemia and lymphoma, including a cutaneous form, occur which to our knowledge have not been subclassified (Olson 1979). (science-connections.com)
  • We therefore investigated whether oleandrin or a N. oleander extract could inhibit virus transmission from a GFP-expressing HTLV-1+ lymphoma T-cell-line to huPBMCs in co-culture assays. (longdom.org)
  • The botanical glycoside, oleandrin, derived from extracts of Nerium oleander , has previously been shown to reduce the levels of the gp120 envelope glycoprotein on human immunodeficiency virus type-1 (HIV-1) particles and inhibit HIV-1 infectivity in vitro . (longdom.org)
  • The botanical glycoside, oleandrin, and an extract of Nerium oleander have been shown to prevent the incorporation of the gp120 envelope glycoprotein of HIV-1 into mature virus particles and inhibit viral infectivity in vitro [ 1 ]. (longdom.org)
  • The identities of these genes provide insights into the mechanism of virus resistance and will help to define new pathways used during retrovirus infection. (biomedcentral.com)
  • We have been involved in the development of screens and selections for virus resistance genes, and have isolated mutant cell lines after chemical mutagenesis that are profoundly resistant to retrovirus infection. (biomedcentral.com)
  • A novel retrovirus, human T -celllymphomaleukemia virus (HTLV), has been isolated from the fresh and cultured cells of two of these patients. (science-connections.com)
  • A retrovirus is a "stealth virus" that, like HIV, enters the host without alerting the immune systems. (nexusnewsfeed.com)
  • in the Journal of Science, describing the first isolation of the recently discovered retrovirus, XMRV, and its association to ME/CFS. (nexusnewsfeed.com)
  • We therefore tested whether oleandrin or an extract from N. oleander could also inhibit the infectivity of the human T-cell leukemia virus type-1 (HTLV-1) -a related enveloped retrovirus and emerging tropical infectious agent. (longdom.org)
  • My principle research interests are in the prevention and therapy of graft-versus-host disease (GVHD) and in the use of graft-versus-leukemia to eradicate blood disorders. (harvard.edu)
  • We have devised a method for the selection of genes from cDNA libraries that suppress the block to virus infection, and so restore virus susceptibility. (biomedcentral.com)
  • The suppressor genes were then recovered from these virus sensitive cells, and their ability to restore virus susceptibility was confirmed by reintroduction of these cDNAs into the resistant line. (biomedcentral.com)
  • These genes have presumably evolved and been maintained in the genome in response to the pathogenic and lethal consequences of infection, and are now thought to constitute an important part of the host defense against these viruses. (biomedcentral.com)
  • We describe the first report of RNA sequencing of 5' capped (Pol II) RNAs isolated from acutely hepatitis C virus (HCV) infected Huh 7.5 cells that provides a general approach to identifying differentially expressed annotated and unannotated genes that participate in viral-host interactions. (mdpi.com)
  • Genetic selections in mammalian cell lines have recently been developed for the isolation of mutant cells that are refractory to infection by retroviruses. (biomedcentral.com)
  • Importantly, these findings suggest oleandrin may have broad antiviral activity against enveloped viruses by reducing the incorporation of the envelope glycoprotein into mature particles, a stage of the infection cycle not targeted by modern HAART. (longdom.org)
  • Herpes zoster is a localized skin infection caused by reactivation of latent varicella-zoster virus. (cdc.gov)
  • Since several animal RNA tumor viruses (Klein 1980) and cells of the putative human retroviruses isolated to date (Bronson et al. (science-connections.com)
  • 3 ] have further demonstrated that oleandrin inhibited tumor growth and disease progression in a murine xenograft model of malignant glioma. (longdom.org)
  • The recent discovery of hepatitis E virus (HEV) strains in rabbits in the People's Republic of China and the United States revealed that rabbits are another noteworthy reservoir of HEV. (cdc.gov)
  • The global epidemiology of Hepatitis C Virus (HCV) may be roughly described by two groups of genotypes: the worldwide distributed ones (subtypes 1a, 1b, 2a and 3a, among others) and the endemic ones (subtypes 4a, 5a, 6a, among others). (cdc.gov)
  • Hepatitis C Virus (HCV) is an enveloped single-stranded positive RNA virus [1] that currently infects approximately 3% of the world's population [2] . (cdc.gov)
  • We describe the almost simultaneous circulation and continuous evolution of genotype VI Newcastle disease viruses in distant locations, suggesting epidemiological connections among three continents. (biomedcentral.com)
  • Evolution of primate oncornaviruses: An endogenous virus from langurs (Presbytis spp. (wikidata.org)
  • A quantitative real time polymerase chain reaction (PCR) revealed canine distemper virus presence in peripheral blood samples from asymptomatic and non vaccinated dogs. (scielo.br)
  • Oncolytic vesicular stomatitis virus (VSV) can be delivered intravenously to target primary and metastatic lesions, but the interaction between human peripheral blood leukocytes (PBLs) and VSV remains poorly understood. (karger.com)
  • However, the collected virus particles from treated cells exhibited reduced infectivity on primary human peripheral blood mononuclear cells (huPBMCs). (longdom.org)
  • 1975) have required the establishment of continuously growing cell lines from the disease of origin or cocultivation of these cells with previously established cell lines, the ability to grow malignant T -cells in long-term culture could facilitate the isolation of retroviruses from these diseased states. (science-connections.com)
  • RNA in mammalian sarcoma virus transformed nonproducer cells homologous to murine leukemia virus RNA. (wikidata.org)
  • The purpose of this assignment was to assess our selected article--Isolation of Potent SARS-CoV-2 Neutralizing Antibodies and Protection from Disease in a Small Animal Model--in terms of accuracy, availability, and reliability. (openwetware.org)
  • Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model. (openwetware.org)
  • Association between the occurrence of antibodies to simian vacuolating virus 40 and bladder cancer in male smokers. (vaccineinjury.info)
  • Murine dermal fibroblasts and Tregs were cocultured with or without blocking antibodies against target cytokines. (biomedcentral.com)
  • Thus, the evolution, current spread and detection of these viruses are relevant to avian health. (biomedcentral.com)
  • Virulent Newcastle disease viruses (NDV), synonymous with avian avulaviruses 1 (AAvV-1), are the causative agents of Newcastle disease (ND). (biomedcentral.com)
  • 1µg RNA was reverse transcribed by using Molone murine leukemia virus (M-MuLV) reverse transcriptase as per manufactures instructions. (ukessays.com)
  • Hence, in our initial attempts to develop cell lines from patients with T -cell neoplasias, we chose clinical subpopulations which represent a more mature form of disease, namely, the cutaneous T -cell lymphomas and leukemias and E-rosette positive T -cell ALL (Gupta and Good 1980). (science-connections.com)
  • The remarkable diversity and mobility of Newcastle disease viruses (NDV) includes virulent viruses of genotype VI. (biomedcentral.com)
  • These viruses are often referred to as pigeon paramyxoviruses 1 because they are normally isolated and cause clinical disease in birds from the Columbidae family. (biomedcentral.com)
  • Genotype VI viruses occasionally infect, and may also cause clinical disease in poultry. (biomedcentral.com)
  • For the purpose of this work, the taxa name Newcastle disease virus will be used. (biomedcentral.com)
  • Canine distemper virus vaccine samples in VERO cells were used as positive controls. (scielo.br)
  • Cherkeziia, SE, et al, "Disorders in the Murine Chromosome Apparatus Induced By Immunization with a Complex of Anti-viral Vaccines," Vopr Virusol, 1979 Sept Oct, (5):547-550. (vaccineinjury.info)
  • Consistent with zoster resolution, viral DNA is largely undetectable on skin from day 90 and virus-specific B and T cells decline in blood. (cdc.gov)
  • We determined the capacity of the leukemia cell line K562 modified to express a membrane-bound form of interleukin (IL)-15 and 41BB ligand (K562-mb15-41BBL) to generate human NK cells with enhanced cytotoxicity. (aacrjournals.org)
  • In several instances these leukemias involve T -cells. (science-connections.com)
  • 4 In addition, TCRs for minor histocompatibility antigens, including HA-1 and HA-2, have been transferred to donor-derived virus-specific T cells to treat patients with hematologic malignancies after allogeneic stem cell transplantation. (haematologica.org)
  • Here, we show virus-specific T cells are retained over one year in serial samples of rash site and contralateral unaffected skin of individuals recovered from zoster. (cdc.gov)
  • In skin, there is selective infiltration and long-term persistence of varicella-zoster virus-specific T cells in the rash siterelative to the contralateral site. (cdc.gov)
  • 197 5b ), rare cases of chronic lymphatic leukemia (Brouet et al. (science-connections.com)
  • led a team that discovered a strong association between a previously unknown retro- virus and myalgic encephalomyelitis, commonly known as chronic fatigue syndrome (ME/CFS). (nexusnewsfeed.com)
  • Here we describe the identification of bottlenecks limiting adeno-associated virus (AAV)-mediated prime editing in vivo and the development of AAV-PE vectors with increased PE expression, prime editing guide RNA stability and modulation of DNA repair. (nature.com)
  • Isolation of virus related to SV40 from patients with progressive multifocal leukoencephalopathy. (vaccineinjury.info)
  • Quantitative results of real time PCR generated by ABI Prism 7000 SDS Software showed that 54.5% of dogs with asymptomatic canine distemper were positive for canine distemper virus. (scielo.br)
  • This virus has a bipartite positive-strand RNA genome consisting of RNA1 and RNA2, which are separately encapsidated in isometric particles of 28 nm in diameter. (ac.ir)
  • At present, there are no antiretroviral drugs that inhibit incorporation of the envelope glycoprotein into newlysynthesized virus particles. (longdom.org)
  • These genotype VI viruses are mainly isolated from Rock Pigeons ( Columba livia ), but also have been isolated from other members of the family Columbidae, e.g. feral Eurasian Collared Doves ( Streptopelia decaocto ) [ 14 ]. (biomedcentral.com)
  • Infectious C-type virus isolated from a baboon placenta. (wikidata.org)
  • A new rapid rRT-PCR test to determine the presence of virulent cleavage sites for viruses from sub-genotypes VIg was developed and evaluated on these and other viruses. (biomedcentral.com)
  • He developed MassTag PCR and Greenechip technology, two multiplex assays that have been used to identify and characterize more than 400 viruses, and was the first to use high throughput sequencing for pathogen discovery. (columbia.edu)
  • This presentation will elaborate on the NIH support for exosome isolation, as well as its potential use in regenerative medicine. (selectbiosciences.com)
  • Geissler, E. "SV40 in human intracranial tumors: passenger virus or oncogenic 'hit-and-run' agent? (vaccineinjury.info)
  • As pigeons are not migratory, this study suggests the need to understand the possible role of human activity in the dispersal of these viruses. (biomedcentral.com)
  • Total RNA was extracted from fresh leaves using Total RNA Isolation Kit (Denazist, Iran) following the manufacturer's instructions. (ac.ir)
  • The recent discoveries of HEV-like viruses in rats and fish have further broadened understanding of the host range and diversity of HEV ( 10 - 12 ). (cdc.gov)