Isolation cell line. Medical search. Frequent questions

Isolation and antigenic characterization of Lassa virus. (wikipedia.org)
Isolation and characterization of sets of anti-L-fucose and anti-bovine serum albumin antibodies directed against a. (aidstar-one.com)
Davis R.E., Worley J.F., Clark T.B., and Moseley M. New spiroplasma in diseased honeybee (Apis melliferum L.): Isolation, pure culture, and partial characterization in vitro. (aaem.pl)
Isolation and characterization of a population of immature dental pulp stem cells expressing OCT-4 and other embryonic stem cell markers. (bvsalud.org)

Isolation of a cDNA clone for tyrosine hydroxylase mRNA. (duke.edu)
Poly(A+)-containing RNA from cells treated with both inducers was used to construct a cDNA library. (duke.edu)
The protocol involves the transformation of pools of resistant cells by cDNA expression libraries, followed by the selection for rare virus-sensitive cells, using multiple rounds of selection after infection by marked viral vector genomes. (biomedcentral.com)
We here report the isolation of two cDNA constructs that each restore virus sensitivity to the R4-7 mutant cell line. (biomedcentral.com)
Northern blot analysis of Jurkat poly(A)+ RNA shows a transcript of 4.7 kb upon probing with the B cell CR2 cDNA. (nih.gov)
Two cDNA clones were identified upon screening of a Jurkat cell cDNA library with the B cell CR2 cDNA. (nih.gov)
RNA Isolation and cDNA Microarrays. (lu.se)

We used 1-day-old cells for measuring median tissue culture infectious dose 50 (TCID50) and microneutralization assay. (cdc.gov)
All isolates were quantitated in Vero E6 cells by TCID50 using the Reed-Muench method based on 8 replicates per titration and propagated to generate sufficient titers ( 100 TCID50 ) for the microneutralization assay. (cdc.gov)
Luciferase reporter assay was performed to verify that miR-455 directly binds to the 3′-noncoding region (UTR) of RAB18 mRNA in Huh7 cells. (hindawi.com)
The cytotoxicity on different cell lines was assayed using the 3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyltetrazolium bromide (MTT) assay which measures survival based on the detection of mitochondrial activity and by the crystal violet assay, which measures survival based on the ability of cells to remain adherent to microplates. (scielo.br)
We also illustrate a novel assay that can identify B cells destined to undergo transformation as early as three days after infection. (jove.com)
PCR assay or culture isolation and proved suitable to probe hydrolysis technology (Applied Biosystems, Foster detect SARS-CoV in clinical specimens. (cdc.gov)

A) shows human embryonic stem cells. (citizendium.org)
Such tissue renewal may be accomplished via the use of adult stem cells, or embryonic stem cells, which may be derived from a human embryo in the blastocyst stage. (citizendium.org)
The use of embryonic stem cells has been a source of considerable controversy due to its sacrifice of human embryos in the blastocyst stage, which some people view as the destruction of human life . (citizendium.org)
This result suggests that the observed increase in motor function was indeed produced by neurons derived from the human embryonic stem cells. (citizendium.org)
Cross-species transplantation was possible without the rejection of the human embryonic stem cells by the mice's immune systems because the mice were genetically modified to suppress certain immune responses that would have interfered with transplantation. (citizendium.org)
Properly controlled, adequately sized studies have yet to demonstrate that human embryonic stem cells have medical value in humans. (citizendium.org)
The most infamous study of embryonic stem cells asserted that cloned human embryos had been created via somatic cell nuclear transfer, and stem cells had been generated from these embryos. (citizendium.org)
Such cells provide a potential alternative to embryonic stem cells. (citizendium.org)
Ethical objections to the use of human embryonic stem cells revolve around the destruction of human embryos in the blastocyst stage to obtain the stem cells. (citizendium.org)
In banning any and all products , food or otherwise, it is essentially banning the importation to Oklahoma of any products made from embryonic stem cells, since one cannot determine which cells are or are not derived from aborted fetuses. (thestranger.com)
Fourier transform infrared microspectroscopy identifies early lineage commitment in differentiating human embryonic stem cells. (edu.au)

Mesenchymal stem cells (MSCs) are multipotent cells with regenerative and immunomodulatory properties. (frontiersin.org)
Human mesenchymal stem cells (HMSCs) are multipotent somatic stem cells that can be isolated from a variety of tissues such as the bone marrow, adipose tissue, and dental pulp. (frontiersin.org)
Many existing tissue-engineering approaches focus on delivery of selected proteins (growth factors, transcription factors etc.) or nucleic acids to host or implanted stem cells to achieve lineage specific differentiation. (frontiersin.org)
The use of the pluripotent and/or self-renewing qualities of stem cells is believed to have therapeutic benefits for the regeneration of tissue in humans. (citizendium.org)
Human embryos fertilized in the ordinary manner and harvested in the blastocyst stage have been used as an extensive source of stem cells for research purposes, and have been shown to possess therapeutic value in laboratory animals. (citizendium.org)
Researchers have recently described new lines of stem cells derived from amniotic fluid [9] . (citizendium.org)
Adult stem cells hold great promise in mitigating much of the ethical debate over embryonic stem cell use. (citizendium.org)
But yeah, this is all about stem cells. (thestranger.com)
Stem cells and regenerative medicine - future perspectives. (springer.com)
Stem cells in regenerative medicine: introduction. (springer.com)
Martin GR. Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. (springer.com)
Adipose tissue-derived mesenchymal stem cells (AD-MSC) show great potential to treat inflammatory and degradative processes in OA and have demonstrated paracrine effects in chondrocytes. (karger.com)
Efficient Endoderm Induction from Human Pluripotent Stem Cells by Logically Directing Signals Controlling Lineage Bifurcations. (edu.au)
The cell population displayed an immunophenotype compatible to the one of stem cells, with remarkable positive expression of CD117. (bvsalud.org)
Dental pulp tissue engineering with stem cells from exfoliated deciduous teeth. (bvsalud.org)
5. Gronthos S, Mankani M, Brahim J, Robey PG, Shi S. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. (bvsalud.org)
Stem cell properties of human dental pulp stem cells. (bvsalud.org)
SHED: stem cells from human exfoliated deciduous teeth. (bvsalud.org)
9. Chen SC, Marino V, Gronthos S, Bartold PM. Location of putative stem cells in human periodontal ligament. (bvsalud.org)
11. Kramer PR, Nares S, Kramer SF, Grogan D, Kaiser M. Mesenchymal stem cells acquire characteristics of cells in the periodontal ligament in vitro. (bvsalud.org)

Establishment of germ-line-competent embryonic stem (ES) cells using differentiation inhibiting activity. (springer.com)
Overexpression of IRS-1 was associated with cell differentiation, whereas GOLPH3 was related to lymph node metastasis, tumor invasion in-depth and TNM stage in ESCC patients. (spandidos-publications.com)
miRNAs are involved in biological and pathological processes including cell differentiation, proliferation, apoptosis and metabolism, and they are emerging as highly tissue-specific biomarkers with potential clinical application for defining cancer types and origins. (spandidos-publications.com)
Comparing these transcriptional responses provides a greater understanding of the mechanisms cells use in the differentiation between types of DNA damage and the potential consequences of different sources of damage. (biomedcentral.com)
The expression of TRA-1-60 antigen is stage-specific and can be used to characterize embryonic cells and monitor their differentiation. (bdbiosciences.com)
As human EC and ES cells undergo differentiation, expression of TRA-1-60 antigen is lost. (bdbiosciences.com)
Light microscopic, morphometric, and immunohistological approaches were used to determine the distribution and degree of differentiation of ciliated and nonciliated bronchiolar epithelial (Clara) cells lining alveolar ducts of the central acinus, a primary target of ozone -induced lung injury. (cdc.gov)

We sent the nasopharyngeal specimens with confirmed RT-PCR and genome sequencing for viral isolation. (cdc.gov)
The viral DNA was apparently trapped in the cytoplasm in a form that was not readily extracted by conditions that allowed DNA recovery from wild-type infected cells. (biomedcentral.com)
Both lines R3-2 and R4-7 were also resistant to infection by pseudotyped HIV-1 viral vectors. (biomedcentral.com)
Like all plus-strand RNA viruses, the SARS-CoV-2 replication process within cells requires de novo production of viral RNA species, including single-strand (ss)RNA and double-strand (ds)RNA that can be sensed by cytosolic pattern recognition receptors (PRRs) subsequently activating antiviral pathways 7 . (nature.com)
In addition to direct viral sensing, cells have also evolved ways to detect the indirect effects of virus infection, such as nuclear or mitochondrial damage caused by the heavy cellular burden imposed by virus replication. (nature.com)
Viral-antigen detection is one of the new tests, but the results are generally less sensitive and less specific than those of conventional cell cultures. (medscape.com)
Viral pneumonia can be diagnosed by isolation and identification of the pathogen through viral culture. (medscape.com)
In this paper we describe the genetic sequence of bovine viral diarrhea virus 1 (BVDV-1) contaminating a tissue culture cell line which was engineered to serve as a system for isolation of foot-and-mouth disease virus, (FMDV). (usda.gov)
Here we report the genome of bovine viral diarrhea virus 1 (BVDV-1) contaminating a continuous fetal bovine kidney cell line which was engineered to express the bovine integrins that serve as a receptor for foot-and-mouth disease virus, (FMDV). (usda.gov)
Adenoviral DNA is retained by peripheral epithelial cells of the lung (PLECs) in humans in whom the viral E1A gene is expressed 4 without replication of a complete virus 5 . (ersjournals.com)

Genetic selections in mammalian cell lines have recently been developed for the isolation of mutant cells that are refractory to infection by retroviruses. (biomedcentral.com)
It is becoming increasingly apparent that mammalian cells harbor numerous genes that induce intracellular blocks to retrovirus infection [ 1 , 2 ]. (biomedcentral.com)
COX-1 is expressed constitutively and is isolated throughout most cell lines in almost all mammalian tissues. (medscape.com)
The CloneSelect® Imager FL is a high-throughput automated solution for imaging and analyzing mammalian cells. (moleculardevices.com)
Viable offspring derived from fetal and adult mammalian cells. (springer.com)

The methods for gene isolation developed here will also permit the identification of similar suppressors that modify or override other recently identified virus resistance genes. (biomedcentral.com)
The manual screening methods traditionally used for cell line development are time-consuming and labor-intensive, creating a great demand for high throughput, automated solutions for such efforts. (moleculardevices.com)
This study was developed to compare the morphological, proliferative and immunophenotypic profiles of pulp cells from permanent and primary teeth, obtained by two isolation methods. (bvsalud.org)
The findings of this study indicate that both isolation methods can be efficiently used. (bvsalud.org)

Cell viability and invasion were measured by CCK8 and Transwell assays. (hindawi.com)
RAB18 reversed partial roles of miR-455 on cell viability and invasion in HCC. (hindawi.com)
miR-455 inhibited cell viability and invasion by directly targeting the 3′-UTR of RAB18 mRNA of hepatocellular carcinoma. (hindawi.com)
In addition, miR-455 inhibited cell viability, while it induced cell apoptosis in colorectal cancer [ 13 ]. (hindawi.com)
Thus, the pivotal roles of miR-455 in cell viability and metastasis in HCC still needed to be explored. (hindawi.com)
The viability of the isolated cells can be maintained for direct identification of any cell characteristics within the device. (rsc.org)
This allows the instrument to preserve cell viability and outgrowth while simultaneously increasing cell deposition efficiency. (moleculardevices.com)
Maximize efficiency, preserve cell viability, and streamline your workflow with DispenCell and CloneSelect Imager FL bundle. (moleculardevices.com)
The A549 cell line was the most sensitive of the cell lines tested with a significant reduction in viability observed at 40 µg/mL. (scielo.br)

Here, we have evaluated transcriptional and cytokine secretion profiles and detected a distinct upregulation of inflammatory cytokines in infected cell cultures and samples taken from infected patients. (nature.com)
Buckley, Sonja M. "Arbovirus infection of vertebrate and insect cell cultures, with special emphasis on Mokola, Obodhiang, and kotonkan viruses of the rabies serogroup. (wikipedia.org)
The changes that occur during adenoviral E1A transformation of primary guinea pig PLECs were also compared with those occurring during the transformation of the fibroblasts that frequently contaminate primary epithelial cultures, and evidence is presented that both cell types are transformed to an intermediate phenotype. (ersjournals.com)
Fully active virus laboratories that maintain the appropriate cell cultures weekly and annually test 150 stool specimens of any origin for any enteric viruses are deemed to meet this criterion. (who.int)
Pulp cells cultures were established by two approaches: enzyme digestion (3 mg/mL type I colagenase and 4 mg/mL dispase), or culture of the tissue explants in cell culture dishes. (bvsalud.org)

Two such lines isolated from a parental fibroblast cell line, Rat2 cells, have been characterized in some detail [ 6 ]. (biomedcentral.com)
Activation of muscle-specific genes in pigment, nerve, fat, liver, and fibroblast cell lines by forced expression of MyoD. (springer.com)

Rational design of chimeric antigen receptor T (CAR-T) cells based on the recognition of antigenic epitopes capable of evoking the most potent CAR activation is an important objective in optimizing immune therapy. (nature.com)
Genotoxic damage causes robust alterations to pathways associated with B cell activation and increased proliferation, suggesting that genotoxic damage initiates not only the normal B cell maturation processes but also mimics activated B cell response to antigenic agents. (biomedcentral.com)
These results suggest genotoxic damage may induce a unique cancer-prone phenotype and processes mimicking activated B cell response to antigenic agents, as well as the normal B cell maturation processes. (biomedcentral.com)

The first-in-human testing of scFv-based B7-H3 CAR-T cells has shown antitumor response against anaplastic meningioma and childhood diffuse intrinsic pontine glioma without severe side effects 16 , 17 . (nature.com)
Our results show that SARS-CoV-2 directs a cGAS-STING mediated, NF-κB-driven inflammatory immune response in human epithelial cells that likely contributes to inflammatory responses seen in patients and could be therapeutically targeted to suppress severe disease symptoms. (nature.com)
Also, in gastric cancer, miR-455 inhibited human cell proliferation and invasion and promoted cell apoptosis [ 12 ]. (hindawi.com)
Our study shows that Jurkat human T cells have a molecule that reacts with both anti-CR2 antibodies and the third component of complement, C3. (nih.gov)
In the present study, we investigated the therapeutic potential of TRAIL in primary human glioma cells. (aacrjournals.org)
We established a stable culture model for isolated primary human glioma cells. (aacrjournals.org)
We verify the cell isolation ability using the human breast cancer cell line MDA-MB-231 with perfect agreement with the microbead results. (rsc.org)
The aim of this paper is to analyse sunitinib malate in vitro ability to enhance cisplatin cytotoxicity in T24, 5637, and HT1376 human urinary bladder-cancer cell lines. (hindawi.com)
This investigation aims to analyse the in vitro effects of cisplatin and sunitinib malate in isolation and in combination, on one human nonmuscle invasive urinary bladder-cancer cell line (5637) and on two human muscle-invasive urinary bladder-cancer cell lines (T24 and HT1376). (hindawi.com)
In conclusion, acetone and methanol extracts from C. odontophyllum stem bark exhibited promising cytotoxic activity against human colorectal cancer cell line HCT 116 and paves way for further investigation in view of its mode of cancer cell death. (scirp.org)
The results indicate that the sea anemone venom is cytotoxic to human cancer cells. (scielo.br)
After sixteen weeks, the injured mice who received human stem cell injections experienced a significant improvement in the motor functions that had been impaired by their injuries. (citizendium.org)
Furthermore, the use of diphtheria toxin -- which is far more toxic to human cells than mouse cells -- to destroy the human neurons in the mice reversed the observed improvements in motor function. (citizendium.org)
However, supporters of embryonic stem cell research frequently contend that even the comparison to abortion is inappropriate, since while a several month old fetus might have sufficient neurological development to be conscious in some meaningful sense, a human embryo in the blastocyst stage has so little development that one can safely conclude that it cannot exist as a conscious being. (citizendium.org)
Embryonic stem cell lines derived from human blastocysts. (springer.com)
Evans MD, Kelley J. US attitudes toward human embryonic stem cell research. (springer.com)
In the present work, we have isolated and characterized the extracellular vesicles from human AD-MSC to investigate their role in the chondroprotective actions of these cells. (karger.com)
Flow cytometric analysis of PE Mouse Anti-Human TRA-1-60 on H9 cells. (bdbiosciences.com)
H9 human embryonic stem (ES) cells were harvested with Accutase™ and stained with either PE Mouse Anti-Human TRA-1-60 (solid line) or PE mouse IgM (G155-228) isotype control (Cat. (bdbiosciences.com)
NKX2-5eGFP/w hESCs for isolation of human cardiac progenitors and cardiomyocytes. (edu.au)
A Targeted NKX2.1 Human Embryonic Stem Cell Reporter Line Enables Identification of Human Basal Forebrain Derivatives. (edu.au)
However, T whipplei bacteria have been grown successfully in HEL (a human fibroblast line) cells. (medscape.com)
Thus, we performed a high-throughput screen for small molecules active against rubella virus in human respiratory cells and identified two nucleoside analogs, NM107 and AT-527, with potent antiviral activity. (cdc.gov)
10. Kamata N, Fujimoto R, Tomonari M, Taki M, Nagayama M, Yasumoto S. Immortalization of human dental papilla, dental pulp, periodontal ligament cells and gingival fibroblasts by telomerase reverse transcriptase. (bvsalud.org)
2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)
CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)

One day before inoculation, we seeded 1.5 mL of cell suspension at a concentration of 3 × 105 cells/mL onto 5.5 cm2 tissue culture tubes. (cdc.gov)
It is described as a housekeeping enzyme, being responsible for cell-to-cell signaling, tissue homeostasis, and cytoprotection. (medscape.com)
Reports from several laboratories suggest that a phenomenon termed epithelial-mesenchymal transformation, in which epithelial cells transform into fibroblasts capable of producing connective tissue matrix, is an important feature of tissue remodelling 10 , 11 . (ersjournals.com)
MiR-21 promoted invasion of fibroblasts by down-regulation of tissue inhibitor of metalloproteinase 3 (TIMP3) expression and increasing of matrix metalloprotein (MMP) expression in fibroblast cells via melanoma-derived exosomes in a time-dependent manner. (dovepress.com)
10 High expression of miR-21 in melanoma cells inhibits mRNA expression of crucial tumor suppressor proteins such as the tissue inhibitor of metalloproteinases 3 (TIMP3). (dovepress.com)
The term stem cell is also used in reference to any adult cells that are capable of assisting in the restoration of adult tissue via self-renewal. (citizendium.org)
A total of 340 clinical specimens collected from 246 were quickly developed: tissue culture isolation, antibody persons with confirmed or suspected SARS-CoV infec- detection, and reverse transcription-polymerase chain tion (13) were used in this study. (cdc.gov)

Totipotent cells have the capacity to differentiate to all cell types, including somatic cells, germ cells, and certain cells that exist outside the embryo and are important to fetal development that are termed extraembryonic cells. (citizendium.org)
1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)

Isolation of amniotic stem cell lines with potential for therapy. (wakehealth.edu)

Xiu Y, Wu T, Meng X, Meng O, Wang W. Identification and isolation of a spiroplasma pathogen from diseased oriental river prawn, Macrobrachium nipponense, in China: A newfreshwater crustacean host. (aaem.pl)

A high degree of heterogeneity in the magnitude of bronchiolar epithelial cell extension into alveolar ducts was noted for each isolation and animal. (cdc.gov)
Age-matched control animals also demonstrated variation in the degree of bronchiolar epithelial cell extension down alveolar ducts. (cdc.gov)
No evidence of inflammation was present in alveolar ducts, suggesting that epithelial cell transformations in alveolar ducts is a natural consequence of lifetime exposures to oxidant gases. (cdc.gov)

Esophageal squamous cell carcinoma (ESCC) is a common histologic subtype in China. (spandidos-publications.com)
Although Barrett's adenocarcinoma is the most rapidly increasing cancer in Western countries, esophageal squamous cell carcinoma (ESCC) remains dominant in East Asia ( 3 ). (spandidos-publications.com)
However, studies have also reported that aberrant overexpression of miR-126 contributes to carcinogenesis, suggesting that miR-126 acts as an oncogene in gastric cancer and oral squamous cell carcinoma ( 16 , 17 ). (spandidos-publications.com)
Objective: To discern long non-coding RNAs (lncRNAs) with prognostic relevance in the context of lung squamous cell carcinoma (LUSC), we intend to predict target genes by leveraging The Cancer Genome Atlas (TCGA) repository. (bvsalud.org)

Single-cell transcriptome RNA sequencing coupled with functional T-cell proteomics analysis uncovers the top-upregulated genes that might be critical for the persistence of polyfunctional CAR-T cells in mice. (nature.com)

The TRA-1-60 monoclonal antibody reacts with the neuraminidase-resistant form of a pluripotent-stem-cell-specific epitope on a high-molecular-weight transmembrane glycoprotein. (bdbiosciences.com)

These selections have been used to recover lines that block early postentry stages of infection, either before reverse transcription or before nuclear entry. (biomedcentral.com)
We have been involved in the development of screens and selections for virus resistance genes, and have isolated mutant cell lines after chemical mutagenesis that are profoundly resistant to retrovirus infection. (biomedcentral.com)
Mutant line R3-2 exhibited a nearly 1000-fold resistance to infection by genetically marked Moloney murine leukemia virus genomes, and was resistant to pseudotyped viruses utilizing the ecotropic envelope, the amphotropic envelope, or even the VSV G envelope protein. (biomedcentral.com)
Mutant line R4-7 exhibited about a 100-fold resistance to infection by M-MuLV, also independent of the envelope mediating entry. (biomedcentral.com)
Infection of this line was blocked earlier, before the initiation of reverse transcription. (biomedcentral.com)
During virus infection, the specific immune signals produced from infected cells are important for dictating the recruitment and activation of innate or adaptive immune cells that are required to fight virus infection. (nature.com)
For SARS-CoV-2, lung epithelial cells are the primary site of infection and therefore are responsible for initiating immune responses to virus infection. (nature.com)
Moreover, cGAS is an important molecule for regulating basal expression levels of cell-intrinsic immune genes in cells and is, therefore, a central protein in immune responses to virus infection 10 . (nature.com)
Buckley, Sonja M. "Susceptibility of the Aedes albopictus and A. aegypti cell lines to infection with arboviruses. (wikipedia.org)
Immunosuppressed persons, such as stem cell transplant or organ transplant recipients, may require CT scanning to clarify upper respiratory versus lower respiratory infection. (medscape.com)
CMV infection characteristically is associated with "owl's-eye" cells, which are large cells with basophilic intranuclear inclusions and a surrounding clear zone. (medscape.com)
To date, Koch's postulates have not been fulfilled completely (infection of an animal model and isolation of the organism from the animal). (medscape.com)
Thus, there are fewer white blood cells to fight infection. (msdmanuals.com)

Cell proliferation and invasion were assayed using the kidney cell lines HEK293 with wild-type p53 and a ccRCC cell line MZ1257RC mutated for p53. (frontiersin.org)

The expression of miR-455 was lower in HCC tissues and cell lines than in nontumor tissues and normal cell line, and downregulation of miR-455 was connected with worse outcome of HCC patients. (hindawi.com)
RAB18 was upregulated in HCC tissues and cell lines, and the expression of RAB18 was regulated by miR-455. (hindawi.com)
Tissues and Cells. (lu.se)
Cells Tissues Organs. (bvsalud.org)
B cells One of the body's lines of defense ( immune system) involves white blood cells (leukocytes) that travel through the bloodstream and into tissues, searching for and attacking microorganisms and. (msdmanuals.com)

miR-455 suppressed cell proliferation in vitro and in vivo, and it inhibited the abilities of cell invasion and EMT in HCC. (hindawi.com)

The effect of miR-126 expression on target proteins was assessed using miR-126 mimics or miR-126 inhibitors in ESCC cell lines. (spandidos-publications.com)

Here, we generate dromedary camel nanobodies targeting B7-H3 and demonstrate that CAR-T cells, based on the nanobodies recognizing the IgC but not IgV domain, had potent antitumour activity against large tumors in female mice. (nature.com)
However, the successful application of these emerging cell-based therapies in solid tumors remains limited. (nature.com)
TRA-1-60 antigen is released into the serum of patients bearing testicular tumors containing EC cells. (bdbiosciences.com)

95% sequential isolation efficiency. (rsc.org)

We have adopted an interdisciplinary approach to disease research, from cell biology to protein expression and chemical synthesis. (edu.au)
BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)

The most potent nanobody-based CAR-T cells show inhibition of large tumor xenografts in mice with rigorous T-cell signaling and significant T-cell infiltration into the tumor. (nature.com)
Furthermore, ΔT2 of the pancreata of prediabetic NOD mice was significantly higher than that of diabetic NOD mice after the injection of MN-Ex10-Cy5.5, indicating the decrease of probe accumulation in these animals due to β-cell loss. (diabetesjournals.org)
The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)

Pluripotent teratocarcinoma-thymus somatic cell hybrids. (springer.com)

We optimize the trappers' dimensions and locations through both computational and experimental analyses using microbeads and cells. (rsc.org)

We have established a permanent cell line (1H91) of putative type-1 astrocyte precursor cells that were clonally derived from a single cell isolated from E16 mouse cerebellum. (illinois.edu)

Pluripotent cells may differentiate to cells of most types, and multipotent cells are capable only of differentiating to certain types within a group of cells that perform similar functions. (citizendium.org)

The suppressor genes were then recovered from these virus sensitive cells, and their ability to restore virus susceptibility was confirmed by reintroduction of these cDNAs into the resistant line. (biomedcentral.com)
To probe the nature of the blocks in these mutant cell lines, we have sought to identify and characterize suppressor genes that override the restriction exhibited by these cells. (biomedcentral.com)
X-Linked Inheritance Genes are segments of deoxyribonucleic acid (DNA) that contain the code for a specific protein that functions in one or more types of cells in the body or code for functional RNA molecules. (msdmanuals.com)

Recently, increasing evidences elucidated that miRNAs was involved in the cancer pathogenesis, including cell proliferation, metastasis, and apoptosis [ 6 ]. (hindawi.com)
The antitumor effect of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) in gliomas has thus far only been thoroughly established in tumor cell lines. (aacrjournals.org)
We isolated primary tumor cells from 13 astrocytoma and oligoastrocytoma patients of all four WHO grades of malignancy and compared the levels of TRAIL-induced apoptosis induction, long-term tumor cell survival, caspase, and caspase target cleavage. (aacrjournals.org)
In addition, the impact of miR-126 on cell proliferation, apoptosis, migration and invasion was detected in ESCC cell lines. (spandidos-publications.com)

Guinea pig primary peripheral lung epithelial cells (PLECs) transfected with adenovirus E1A (E1A-PLECs) were compared to guinea pig normal lung fibroblasts (NLFs) transfected with E1A (E1A-NLFs). (ersjournals.com)
E1A transfection results in the transformation of peripheral lung epithelial cells and normal lung fibroblasts to a phenotype intermediate between that of the two primary cells. (ersjournals.com)
Case-control studies have shown that the E1A DNA from group C adenoviruses is present in greater amounts in the lungs of patients with COPD than in controls with normal lung function matched for age, sex and smoking history 5 , and that the excess inflammation in the lungs of smokers with severe emphysema is associated with increased numbers of alveolar epithelial cells expressing E1A 6 . (ersjournals.com)
In the present study, the venoms from five sea anemones (Heteractis crispa, Heteractis magnifica, Heteractis malu, Cryptodendrum adhaesivum and Entacmaea quadricolor) were obtained by the milking technique, and the potential of these venoms to kill cancer cells was tested on three cell lines (A549 lung cancer, T47D breast cancer and A431 skin cancer). (scielo.br)
Studies have shown that the expression of miR-126 is downregulated in cancers such as non-small cell lung cancer and colon cancer ( 14 , 15 ), suggesting that the downregulation of miR-126 is significantly related to the occurrence and development of cancer. (spandidos-publications.com)

2 , 3 Tumor cell-derived exosomes carry particular molecular components originated from donor cells to mediate tumorigenesis and drive tumor progression by regulating tumor microenvironment. (dovepress.com)

The type I/III IFN pathways are directly involved in protecting neighboring cells from virus spread and are vital for the immediate cell-intrinsic antiviral response. (nature.com)

Recent findings in our laboratory and those of others, however, have shown that the EBV receptor is also present on T cells. (nih.gov)
Toward this goal, we synthesized an exendin-4 conjugated magnetic iron oxide-based nanoparticle probe targeting glucagon-like peptide 1 receptor (GLP-1R), which is highly expressed on the surface of pancreatic β-cells. (diabetesjournals.org)
It is also responsible for inhibition of vascular endothelial growth factors receptors, plateletderived growth factor receptors, and stem cell factor receptor [ 8 , 9 ]. (hindawi.com)

Featuring high-contrast multichannel fluorescent and white light imaging that allows for accurate single-cell detection and proof of monoclonality at day 0, the instrument allows you to streamline your workflow with comparative confluence assays to identify and verify gene edits. (moleculardevices.com)
It has been reported that miR-126 is located on chromosome 9q34.3 within the host gene encoding for epidermal growth factor like-7 (EGFL-7), an endothelial cell-derived, secreted inhibitor of smooth muscle cell migration and a regulator of blood vessel formation ( 12 , 13 ). (spandidos-publications.com)
The primitive streak gene Mixl1 is required for efficient haematopoiesis and BMP4-induced ventral mesoderm patterning in differentiating ES cells. (edu.au)
Treatment involves antibiotics to prevent infections and immune globulin, but the only effective treatments are stem cell transplantation and possibly gene therapy. (msdmanuals.com)

The antigen is found on teratocarcinoma (embryonal carcinoma or EC), embryonic inner cell mass (but not morula or trophoblast), and embryonic stem (ES) cells. (bdbiosciences.com)

The DispenCell™ Single-Cell Dispenser is an automated laboratory instrument designed for fast, easy and gentle single-cell isolation. (moleculardevices.com)
Ideally cell line sensitivity should be known for all frozen stocks and evaluated whenever fresh cells are resuscitated or received in the laboratory. (who.int)

Immunoprecipitation experiments with anti-CR2 antibodies and SDS-PAGE analysis reveal a protein with an apparent molecular mass of 155 kDa which is higher than the one seen in B cells. (nih.gov)
resulting in low levels of antibodies (immunoglobulins) and low or no T cells (lymphocytes). (msdmanuals.com)

Transplantation of living nuclei from blastula cells into enucleated frogs' eggs. (springer.com)

Therefore, the 1H91 clonal cell line may provide an in vitro model for studying the underlying cellular mechanisms of the type-1 astrocyte in reactive gliosis. (illinois.edu)
It enables researchers to isolate and dispense single cells for downstream applications such as clonal expansion, monoclonality assurance, and single-cell genomics. (moleculardevices.com)

Isolation of rare cells, such as circulating tumor cells, has been challenging because of their low abundance and limited timeframes of expressions of relevant cell characteristics. (rsc.org)
In animals exposed to ozone , a striking similarity was noted by scanning electron microscopy in the surface characteristics of cells lining both terminal bronchioles and alveolar ducts. (cdc.gov)

In contrast to cell lines, isolated primary tumor cells from all investigated glioma patients were highly TRAIL resistant. (aacrjournals.org)
We further demonstrate that this device can be applied to isolate the largest particles from a sample containing multiple sizes of particles, revealing its possible applicability in isolation of circulating tumor cells in cancer patients' blood. (rsc.org)
Isolation of infected patients and nonreuse of a dialyzer by the same patient were initially recommended for patients receiving dialysis in dialysis centers (2). (cdc.gov)

Here, we report a surface enhanced Raman scattering technology for rapid and label-free exosomal detection (Exo-SERS) to aid in the discrimination of different cancer cells based on specific Raman phenotypes and multivariate statistical analysis. (mdpi.com)
The results demonstrated that exosomes derived from both tumor cells and normal cells exhibit special, unique Raman phenotypes. (mdpi.com)

A patented disposable tip ensures clean isolation of single cells and no cross contamination. (moleculardevices.com)
The dialyzer, along with the associated blood lines, is disposable. (cdc.gov)

We observed the cytopathic effect of virus-infected cells daily. (cdc.gov)

In vitro studies in βTC-6, the β-cell line, showed specific accumulation of the targeted probe (termed MN-Ex10-Cy5.5) compared with nontargeted (termed MN-Cy5.5). (diabetesjournals.org)

Scholars@Duke publication: Regulation of tyrosine hydroxylase mRNA by glucocorticoid and cyclic AMP in a rat pheochromocytoma cell line. (duke.edu)
Treatment of a subclone of the PC12 pheochromocytoma cell line, PC8b, with either dexamethasone or 8-bromo cyclic AMP resulted in increased translational activity of tyrosine hydroxylase mRNA (mRNATH). (duke.edu)
This size is consistent with that of B cell CR2 mRNA. (nih.gov)
Analysis of Jurkat and Raji mRNA by PCR demonstrated the presence of this novel splice variant in both cell lines. (nih.gov)

Cytoplasmic inclusions usually are present in cells infected with RNA viruses. (medscape.com)
I present evidence from reciprocal crosses, cytoplasmic substitution lines, and cell fusion lines that hybrid weakness and sterility often arise from interactions between the nuclear genome and the chloroplast and mitochondrial genomes. (bioone.org)

The majority of EGF-treated 1H91 cells are not immunoreactive for glial acid fibrillary protein (GFAP). (illinois.edu)
Furthermore, E1A DNA and protein persisted in epithelial cells in the lungs of these animals 9 . (ersjournals.com)
miR-126 mimics downregulated the expression of IRS-1 and GOLPH3 protein and suppressed the proliferation, migration and invasion of ESCC cells, whereas miR-126 inhibitors led to the opposite results. (spandidos-publications.com)
The presence of Clara cell secretory protein in cells of bronchioles and alveolar ducts was also detected immunohistochemically and visualized using confocal laser scanning microscopy in the reflectance mode. (cdc.gov)

Intranuclear inclusions often exist in cells infected with DNA viruses. (medscape.com)

We separately inoculated 150 L of specimen into Vero E6 cells monolayer. (cdc.gov)

Anatomy32

Organisms18

Diseases26

Chemicals and Drugs63

Analytical, Diagnostic and Therapeutic Techniques and Equipment49

Psychiatry and Psychology1

Phenomena and Processes69

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena1

Information Science4

Health Care2

Characterization4

CDNA7

Assay6

Embryonic stem11

Stem cells20

Differentiation7

Viral10

Mammalian5

Methods4

Viability9

Cultures5

Fibroblast cell2

Antigenic3

Human26

Tissue7

Fetal2

Amniotic1

Pathogen1

Epithelial cell3

Squamous cell carc4

Proteomics1

Antibody1

Infection13

Proliferation and invasion1

Tissues5

Vitro and in vivo1

Proteins1

Tumors3

Efficiency1

Biology2

Mice3

Somatic1

Optimize1

Precursor cells1

Multipotent cells1

Genes3

Apoptosis4

Lung5

Tumor microenvironment1

Pathways1

Receptor3

Gene4

Teratocarcinoma1

Laboratory2

Antibodies2

Transplantation1

Clonal2

Characteristics2

Patients3

Phenotypes2

Disposable2

Cytopathic effect1

Probe1

MRNA4

Cytoplasmic2

Protein4

Viruses1

Monolayer1