• The isolation, characterization, and expression of a full-length cDNA encoding the human T cell glycoprotein CD6 is described. (embl.de)
  • Fabrizio Gianguzza , Maria Antonietta Ragusa , Maria Carmela Roccheri , Italia Di Liegro , and Anna Maria Rinaldi "Isolation and characterization of a Paracentrotus lividus cDNA encoding a stress-inducible chaperonin," Cell Stress & Chaperones 5(2), 87-89, (1 April 2000). (bioone.org)
  • The paper describes the isolation and characterization of a novel pelagic bacterial strain capable of hepatotoxic microcystin (MC) degradation. (degruyter.com)
  • The amino acid sequence of human rhodopsin, deduced from the nucleotide sequence of its gene, is 348 residues long and is 93.4% homologous to that of bovine rhodopsin. (nih.gov)
  • A cDNA clone encoding a protein consisting of 189 amino acid residues including a sodefrin sequence was isolated from a Cynops pyrrhogaster abdominal gland cDNA library. (wiley.com)
  • This clone encoded a precursor protein of 192 amino acid residues, including a sodefrin-like peptide sequence with substitutions of two amino acid residues. (wiley.com)
  • This confirmed the identity of the cDNA clone and established that ribophorin I contains 583 amino acids and is synthesized with a cleavable amino terminal insertion signal of 22 residues. (rupress.org)
  • The deduced amino acid sequence of PPS1p shows similarity with protein-tyrosine phosphatases (PTPases) and is most closely related to a subfamily of PTPases that are capable of dephosphorylating phosphoseryl and phosphothreonyl residues as well as phosphotyrosyl residues. (embl.de)
  • In addition, a distribution of amino acid residues at a particular site is represented geographically to trace the location where antigenic variants are seeded. (biomedcentral.com)
  • The NH2-terminal amino acid sequences of rabbit liver isozymes were identified up to 30 residues, and showed some differences from those of the corresponding isozymes obtained from other animals so far studied. (dbcls.jp)
  • One of the clones possesses an identical nucleotide sequence to the one corresponding to B cell CR2, whereas the other represents a differentially spliced transcript which lacks the exon 8b of B cell CR2. (nih.gov)
  • In silico identification by nucleotide sequence alignment between genomic and cDNA sequences revealed three putative introns in GBSS I. The longest intron was 121 bp long and was located between nucleotide 595 and 716. (scialert.net)
  • Nucleotide sequence of a human cytomegalovirus DNA fragment encoding a 67-kilodalton phosphorylated viral protein. (microbiologyresearch.org)
  • The complete amino acid sequence of the polypeptide encoded in the cDNA insert was derived from the nucleotide sequence and found to contain a segment that corresponds to a partial amino terminal sequence of ribophorin I that was obtained by Edman degradation. (rupress.org)
  • They will understand the methods of determination of amino acid and nucleotide sequence of proteins and DNA respectively. (cutm.ac.in)
  • As a sub-objective, since PDE10A transcript variants were reported strictly through analyses of bovine genomic sequence, we also wanted to determine the nucleotide and amino acid sequences by experimental evidence. (plos.org)
  • Three partial genomic clones were generated and the sequence analysis revealed that they were identical at both nucleotide and amino acid levels. (scialert.net)
  • 7536 /organism="Norovirus Hu/MK04/2004/JP" /mol_type="genomic RNA" /strain="Hu/MK04/2004/JP" /isolation_source="stool specimen" /db_xref="taxon:379081" /country="Japan: Tokyo" CDS 5. (cdc.gov)
  • Furthermore, using sequence analysis of human genomic DNA, researchers concluded that the amino acids important for catalysis by COX-1 are conserved and are equally important for catalysis by COX-2. (medscape.com)
  • As authorities braced for the arrival of the Omicron variant of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), infrastructure investments and government directives prompted action in central Viet Nam to establish capacity for genomic surveillance sequencing. (who.int)
  • Recognizing this, capacity building for genomic flights to Viet Nam underwent testing using real-time surveillance towards a National Sequencing Network reverse transcription polymerase chain reaction (RT-PCR) has become a principal goal in Viet Nam. (who.int)
  • 2 These provisions al owed for to emerging public health threats, determine outbreak quick detection and isolation of cases, as well as targeted etiologies and enhance overall surveillance capacities in genomic surveil ance for imported cases. (who.int)
  • Nucleic Acids Res. (wikipedia.org)
  • Diazotizable arylamine cellulose papers for the coupling and hybridization of nucleic acids. (wikidata.org)
  • This over produced AOS damages/ oxidizes to lipids, proteins and nucleic acids, and releases a number of cytotoxic intermediate/ end products thereby alterations in metabolic pathways and inactivation of key enzymes. (degruyter.com)
  • Module-VII Nucleic acids:Isolation of DNA and RNA. (cutm.ac.in)
  • In addition, 2 addi- in brain tissue suggests that USUV tissue samples was not successful be- tional unique amino acid substitutions might have a higher tropism for the cause of autolysis. (cdc.gov)
  • Amino acid substitutions of the HA were deemed to be positively selected to reduce antibody binding and therefore were supposed to be responsible for driving antigenic drift. (biomedcentral.com)
  • in December 2006, UniMod lists 495 modifications including 144 amino acid substitutions [6]. (lu.se)
  • The aim of this study is to investigate the adaptation of bifidobacterial species to various hosts and to examine the genetic biodiversity of bifidobacterial species from various hosts like human, non-human primates, bat and other organisms by decoding genome sequences. (soken.ac.jp)
  • Apart from this study, we also tested the own, blank, BLAST dataset and annotated whole-genome rhodopsin sequences of PWS haloarchaeal isolates in the developed methods. (biomedcentral.com)
  • Whole-genome sequence analysis of 66 emm4 isolates, 40 isolates from the pre-coronavirus disease 2019 (COVID-19) pandemic period 2009-2019 and 26 contemporary isolates from 2022, identified a novel Streptococcus pyogenes lineage (M4NL22), which accounted for 85â % of emm4 iGAS cases in 2022. (bvsalud.org)
  • Three genome sequences of Buffalopox virus (BPVX) were retrieved from a human and two buffaloes scab samples. (cdc.gov)
  • Further analysis indicated that these two proteins possessed almost identical gene sequences, apart from two insertion/deletion events in predicted external loops present within the putative barrel-like structure of the proteins. (eur.nl)
  • The described cDNA corresponds to a full-length mRNA encoding a protein of 582 amino acids, the first 32 of which constitute a putative mitochondrial targeting leader sequence. (bioone.org)
  • Although the detected detection, histologic analysis, and bat quito and bird-derived strains showed bat-derived sequences are somehow species determination. (cdc.gov)
  • Quantitation of hemoglobin adducts of EO is performed using octapeptides with the same amino acid sequence as the N-terminal of the beta-chain of hemoglobin with EO attached at the valine residue. (cdc.gov)
  • By analyzing the frequency of each amino acid residue of the HA1 domain expressed by the viruses on annual basis, users are able to obtain evolutionary dynamics of human influenza viruses corresponding with epidemics. (biomedcentral.com)
  • Therefore, these toxins cleave Dsg-1 at the N-terminus of glutamic acid residue 381 between extracellular domains 3 and 4. (medscape.com)
  • The transcription of COX-1 yields a 2.7-kilobase (kb) messenger ribonucleic acid (mRNA) that encodes a 576-residue, 65-kd protein. (medscape.com)
  • Isolation and partial amino acid sequence of bacteriocins produced by Lactobacillus acidophilus. (dsmz.de)
  • Isolation and Partial Amino Add Sequence of Bacteriocins Produced by Lactobacillus acidophilus. (dsmz.de)
  • To determine the competency of these bats to act as a natural reservoir host for SOSV capable of infecting humans, captive-bred ERBs were inoculated with a recombinant SOSV, representative of the patient's virus sequence. (cdc.gov)
  • Analysis of the amino acid sequence of ribophorin I suggested that the polypeptide has a simple transmembrane disposition with a rather hydrophilic carboxy terminal segment of 150 amino acids exposed on the cytoplasmic face of the membrane, and a luminal domain of 414 amino acids containing three potential N-glycosylation sites. (rupress.org)
  • Sequence analysis revealed that FPR2 is 69% identical in sequence to the human NFPR and shares extensive homology to several other chemoattractant receptors. (unm.edu)
  • Tree was constructed by merging the common protein sequences in all the strains. (soken.ac.jp)
  • Module -IV Proteins: Isolation, methods of purification-dialysis salting out, pH precipitation and solventprecipitation. (cutm.ac.in)
  • We have isolated and completely sequenced the gene encoding human rhodopsin. (nih.gov)
  • The industrial application of Ion-pumping Haloarchaeal rhodopsins is limited by the lack of full-length rhodopsin sequence-based classifications, which play an important role in Ion-pumping activity. (biomedcentral.com)
  • As a result, a low-cost computational approach is required to identify Ion-pumping Haloarchaeal rhodopsin sequences and its subtype. (biomedcentral.com)
  • The overall performance of the developed method results show that it accurately identifies the Ionpumping Haloarchaeal rhodopsin and their subtypes using known and unknown microbial rhodopsin sequences. (biomedcentral.com)
  • The amino-terminal sequence of β-galactosidase, the α-peptide involved in α-complementation, participates in a subunit interface. (wikidoc.org)
  • These structural features provide a rationale for the phenomenon of α-complementation, where the deletion of the amino-terminal segment results in the formation of an inactive dimer. (wikidoc.org)
  • terminal (C-tcrminal) amino acids. (equimount.com)
  • N-terminal amino acid sequence of the purified protein was determined as SQLKAXIXDF. (uni-mysore.ac.in)
  • Analysis of the predicted amino acid sequence suggests that the protein consists of an active phosphatase domain, an inactive phosphatase-like domain, and an NH2-terminal extension. (embl.de)
  • Primary Structure of proteins, methods of determining N- and C- terminal aminoacids, amino acid composition.Sequencing by Edman's degradation method. (cutm.ac.in)
  • Use of a cytochrome (A1841V and K1870M) in the NS3 nervous system in bats, as opposed b-specific PCR and direct sequencing protein gene of the BAT1USUTU-BNI to the pantropism observed in birds of the PCR amplicons genetically iden- strain were also identified. (cdc.gov)
  • The ET-A and ET-B amino acid sequences are approximately 40% identical with each other. (medscape.com)
  • Phylogenetic tree of genus Bifidobacterium based on the concatenated amino acid sequences of 355 core genes of all type strains. (soken.ac.jp)
  • Quantitative real-time PCR was used to test the expression of ZmCAMTA genes under several abiotic stresses (drought, salt, and cold), various stress-related hormones [abscisic acid, auxin, salicylic acid (SA), and jasmonic acid] and biotic stress [rice black-streaked dwarf virus (RBSDV) infection]. (frontiersin.org)
  • USUV the bat USUV strains in cell culture used more available sequences from circulates between ornithophilic were not successful, probably because databases yielded the same topology mosquito vectors (mainly Culex spp. (cdc.gov)
  • Phylogenetic studies have also designated SaV clusters or genotypes to further describe strains that differ by 10% in nucleotide or amino acid sequences. (cdc.gov)
  • Over the past few years we have had frequent requests for the details such as isolation and attenuation and accordingly we felt that bringing the data together in the report below would be both helpful and informative to those involved in the production and control of poliovirus vaccine (oral) prepared from these strains. (who.int)
  • Accumulation of ascorbic acid, carotenoids, phenols and proline, and exogenous addition of calcium, polyamines, growth tonic, salicylic acid and, β-aminobutyric acid were shown to be the effective strategies to cope low-pH stress in plants. (degruyter.com)
  • Funk and co-investigators localized COX-1 to 9q32-q33.3 via somatic hybrid deoxyribonucleic acid (DNA) analysis. (medscape.com)
  • The new virus shares approximately 75 percent of its nucleotides and 85 percent of its amino acids with Asian and European hantaviruses. (sciencedaily.com)
  • From 17 November 2021 to 7 January 2022, the Pasteur Institute in Nha Trang sequenced 162 specimens from 98 150 confirmed SARS-CoV-2 cases in the region collected from 8 November to 31 December 2021. (who.int)
  • The aim of (requirement expired 15 May 2022) and mandatory the network is to standardize sequencing to empower 14-day quarantine in centralized facilities (requirement the Ministry of Health (MoH) to detect and respond expired 1 January 2022). (who.int)
  • Two decades later, the prostaglandins were deduced to be a family of related compounds that contain 20-carbon polyunsaturated fatty acids with a cyclopentane ring, as depicted below. (medscape.com)
  • By 1964, after recognition of this basic structure, Bergstrom and colleagues successfully synthesized series 2 prostaglandins from arachidonic acid using sheep seminal fluid. (medscape.com)
  • Sequence analysis revealed one of these components to be bovine RNase A, originating from the culture medium and explaining the RNA hydrolyzing activities of Sso7 preparations previously described. (ox.ac.uk)
  • Sequences were sequences in GenBank indicated that all 7 isolates closely aligned with Clustal X ( 9 ), and distances were calculated matched previously reported SaV sequences (Figure 2). (cdc.gov)
  • Isolation of a cDNA that encodes a novel granulocyte N-formyl peptide receptor. (unm.edu)
  • We achieved overall maximum accuracy of 97.78%, 97.84% and 97.60%, respectively, for amino acid composition, dipeptide composition and hybrid approach on tenfold cross validation using SVM. (biomedcentral.com)
  • 0.6% divergent (from PV2) in VP1 nucleotide sequences from the corresponding OPV strain ( 4 ). (cdc.gov)
  • Each strain is highlighted by the color representing its isolation source. (soken.ac.jp)
  • 2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . (wikipedia.org)
  • 2003). "The DNA sequence and analysis of human chromosome 6" . (wikipedia.org)
  • We therefore construct a web server named Fluctrl that implements a pipeline whereby human influenza HA viral sequences were downloaded from the NCBI database and analyzed. (biomedcentral.com)
  • For each subtype of human influenza A virus, sequences were aligned against the reference sequences, A/Puerto Rico/8/34 (YP_163735), A/California/07/2009 (ACP41953) and A/Hong Kong/1/1968 (ACC66318), respectively, by utilizing MUSCLE [ 6 ]. (biomedcentral.com)
  • We also developed an RBD-human IgG1 Fc vaccine with an RBD sequence of the immuno-evasive beta variant (N501Y, E484K, K417N). (bvsalud.org)
  • The rabbit liver isozymes were closely similar to the corresponding isozymes from other sources, including human heart, pig heart, chicken heart, and rat liver, in their molecular weights, absorption spectra, amino acid compositions, isoelectric points, and Michaelis constants for the substrates. (dbcls.jp)
  • Isolation and phylogenomic analysis of Buffalopox virus from Human and Buffaloes in India. (cdc.gov)
  • We have constructed a web server, named Fluctrl, and implemented a pipeline whereby HA sequence data is downloaded from the Influenza Virus Resource at NCBI along with their isolation information including isolation year and location, which are parsed and managed in MySQL database. (biomedcentral.com)
  • Fatty acids - Nomenclature of saturated and unsaturated fatty acids. (cutm.ac.in)
  • Physiological properties of fatty acids. (cutm.ac.in)
  • Therefore, COX - also known as prostaglandin-endoperoxide synthase (PTGS), fatty acid COX, prostaglandin H (PGH) synthase, and EC 1.14.99.1 - is implicated in the production of fever, inflammation, and pain. (medscape.com)
  • Twenty-carbon polyunsaturated fatty acid with cyclopentane ring. (medscape.com)
  • Their distinct biosynthetic activity includes an endoperoxidase synthase reaction that oxygenates and cyclizes polyunsaturated fatty acid precursors (eg, arachidonic acid) to form prostaglandin G 2 (PGG2), and a peroxidase reaction that converts PGG2 to prostaglandin H 2 (PGH2), as shown below. (medscape.com)
  • Users are able to upload and analyze their HA1 sequences for generating evolutionary dynamics. (biomedcentral.com)
  • PTMs from this list in conjunction with a protein sequence and a few precursor masses. (lu.se)
  • Deepcell uses AI for cell classification and isolation in basic and translational research. (biospace.com)
  • Module-III Amino acids: Structure and classification of amino acids based on polarity. (cutm.ac.in)