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ElectrophoresisSeparationPreparativeSerumProteinsOligoclonalMethodologyGelsFingerprintGradientsMoleculesExperimentsPointEfficientlyHandbook Of Isoelectric Focusing And ProteomicsHPLCCapillaryProteomicsProteinPolyacrylamideAgarose gelsSeparationRecombinantDetectionMigrationPurificationAnalysisMolecularResults
Electrophoresis5
  • Isoelectric focusing is the first step in two-dimensional gel electrophoresis, in which proteins are first separated by their pI value and then further separated by molecular weight through SDS-PAGE. (wikipedia.org)
  • Isoelectric focusing is the only step in preparative one-dimensional gel electrophoresis. (wikipedia.org)
  • Carbonic Anhydrase I from human erythrocytes has been used as a pI (isoelectric point) marker in two-dimensional gel electrophoresis. (sigmaaldrich.com)
  • Both lipids and glycogen are known to interfere with the first step in the two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), the isoelectric focusing. (hindawi.com)
  • Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point. (bvsalud.org)
Separation6
  • 2DE analysis of alkaline proteins is challenging due to the difficult separation of basic proteins during the isoelectric focusing (IEF). (nih.gov)
  • My colleagues and I recently developed a method to couple immunoaffinity capture and separation by isoelectric focusing in a single capillary. (nii.ac.jp)
  • The method allows sample pretreatment, i.e., purification and concentration, in a single capillary, in which subsequent separation by isoelectric focusing is achieved. (nii.ac.jp)
  • Evaluation of protein loading techniques and improved separation in OFFGEL isoelectric focusing. (mpg.de)
  • Salts, ionic molecules and ionic detergents interfere with isoelectric focusing and compromise protein separation. (thermofisher.com)
  • Inactivation of the catalytic activity with organophosphate pesticides did not change the isoelectric point of the isozymes, and therefore separation of native from organophosphate inhibited AChE did not occur. (cdc.gov)
Preparative1
Serum2
  • Isoelectric focusing (IEF) of serum transferrin (Tf) is still the method of choice for diagnosis of congenital disorders of glycosylation (CDG). (nih.gov)
  • The method consisted of isoelectric focusing (IEF) of dog serum, followed by immunoblotting. (lu.se)
Proteins5
  • Isoelectric focusing can resolve proteins that differ in pI value by as little as 0.01. (wikipedia.org)
  • According to some opinions, living eukaryotic cells perform isoelectric focusing of proteins in their interior to overcome a limitation of the rate of metabolic reaction by diffusion of enzymes and their reactants, and to regulate the rate of particular biochemical processes. (wikipedia.org)
  • The detergent-based membrane protein preparation protocol not only extracts proteins effectively from cell pellets but also is compatible with subsequent proteome analysis using combined capillary isoelctric focusing/nano reversed-phase liquid chromatography separations coupled with nano electrospray ionization mass spectrometry. (arizona.edu)
  • In the first-dimension, proteins are separated according to their isoelectric point (pI)-the pH at which the protein net charge is zero-in a process known as isoelectric focusing (IEF). (thermofisher.com)
  • During conventional IEF, amphoteric molecules, like proteins, migrate until they reach a region where the pH of the matrix matches their isoelectric point and they "focus" into sharp bands. (thermofisher.com)
Oligoclonal2
  • Isoelectric focusing and immunoblotting studies revealed oligoclonal IgG bands in the aqueous of 13 of 23 (57%) patients with FHC, most being of the IgG1 subclass. (birmingham.ac.uk)
  • 3. Positive findings in a cerebrospinal fluid (CSF) specimen (isoelectric focusing evidence of oligoclonal bands and/or elevated immunoglobulin (Ig) G index) [If a recent CSF sample or results from a previous CSF test from each patient is not available, this data will be considered missing information]. (who.int)
Methodology2
  • Isoelectric focusing methodology using LKB 2117 Multiphor 11 electrofocusing apparatus was used for electofocusing and identification of Oreochromis niloticus and Tilapia zilli. (ajol.info)
  • isoelectric focusing : theory, methodology and applications / Pier Giorgio Righetti. (ua.pt)
Gels1
  • A procedure to detect raw and gelatinized starch activities of glucoamylase on isoelectric focusing (IEF) gels by using 2, 3, 5-triphenyltetrazolium chloride is described. (uni-mysore.ac.in)
Fingerprint1
  • Dive into the research topics of 'Membrane proteome analysis of microdissected ovarian tumor tissues using capillary isoelectric focusing/reversed-phase liquid chromatography-tandem MS'. Together they form a unique fingerprint. (arizona.edu)
Gradients1
  • A system using immobilized pH gradients for isoelectric focusing (IEF) was also developed providing greater reproducibility of 2-dimensional patterns. (europa.eu)
Molecules3
  • Isoelectric focusing (IEF), also known as electrofocusing, is a technique for separating different molecules by differences in their isoelectric point (pI). (wikipedia.org)
  • As a particle moves toward the pole opposite of its charge it moves through the changing pH gradient until it reaches a point in which the pH of that molecules isoelectric point is reached. (wikipedia.org)
  • By modification of the isoelectric point (pI) of molecules of an enzyme by, e.g., phosphorylation or dephosphorylation, the cell can transfer molecules of the enzyme between different parts of its interior, to switch on or switch off particular biochemical processes. (wikipedia.org)
Experiments1
  • Carbonic Anhydrase I from human erythrocytes is a protein which is used as a standard pI (isoelectric point) marker for isoelectric focussing experiments. (sigmaaldrich.com)
Point2
  • A protein that is in a pH region below its isoelectric point (pI) will be positively charged and so will migrate toward the cathode (negatively charged electrode). (wikipedia.org)
  • Small changes in the protein mass or isoelectric point (pI) translate into a detectable protein shift. (thermofisher.com)
Efficiently1
  • Capillary isoelectric focusing efficiently analyze heterogeneity of protein drugs. (nii.ac.jp)
Handbook Of Isoelectric Focusing And Proteomics2
  • Otto not are an British download handbook of isoelectric focusing and proteomics 2005 of what Description jeweils somatically Then. (erzebet.com.ar)
  • The Regent Taktse Shabdrung and little songs n't was a download handbook of isoelectric focusing and proteomics 2005 to the clinical political pp. that they was Kelsang Gyatso as the Dalai Lama. (erzebet.com.ar)
HPLC2
  • Isoelectric focusing nonporous RP HPLC: a two-dimensional liquid-phase separation method for mapping of cellular proteins with identification using MALDI-TOF mass spectrometry. (nih.gov)
  • The proteins are separated by pI using isoelectric focusing in the first dimension and by hydrophobicity using nonporous reversed-phase HPLC in the second dimension (IEF-NP RP HPLC). (nih.gov)
Capillary4
  • Highly sensitive and specific protein detection via combined capillary isoelectric focusing and proximity ligation. (scilifelab.se)
  • Capillary isoelectric focusing together with antibody-based detection can resolve and identify proteins and their isoforms with modest sample input. (scilifelab.se)
  • Here we applied these DNA-assisted RCA techniques in capillary isoelectric focusing to resolve endogenous signaling transducers and isoforms along vascular endothelial growth factor (VEGF) signaling pathways at concentrations too low to be detected in standard assays. (scilifelab.se)
  • 17. Tissue proteomics using capillary isoelectric focusing-based multidimensional separations. (nih.gov)
Proteomics1
  • A proteomics platform combining depletion, multi-lectin affinity chromatography (M-LAC), and isoelectric focusing to study the breast cancer proteome. (nih.gov)
Protein3
  • A protein that is in a pH region below its isoelectric point (pI) will be positively charged and so will migrate toward the cathode (negatively charged electrode). (wikipedia.org)
  • A universal indicator dye demonstrates the generation of stable, reversible pH gradients (3-10) in ampholyte buffers, and these gradients lead to protein focusing within the drop length. (elsevierpure.com)
  • The reduced protein had a pI of 5.1 as judged by isoelectric focusing. (nih.gov)
Polyacrylamide1
  • All three preparations were fractionated by membrane or Sephadex ultrafiltration and iso-electric focusing in polyacrylamide gels, as well as being examined by immunoelectrophoretic techniques. (gla.ac.uk)
Agarose gels2
  • and in isoelectric focusing to minimize endoosmotic flow in agarose gels. (sigmaaldrich.com)
  • There was a complete agreement that isoelectric focusing on agarose gels followed by immunoblotting should be the "gold standard" for detecting the presence of oligoclonal bands. (sebia.com)
Separation2
  • Since beta-lactamases may be universally produced by bacteria, separation of the enzymes by analytical isoelectric focusing could be used in bacterial taxonomy. (nih.gov)
  • The collected glycoproteomes were then subjected to isoelectric focusing (IEF) separation by a digital ProteomeChip (dPC), followed by in-gel digestion and LC-MS analysis using an Orbitrap mass spectrometer. (nih.gov)
Recombinant1
  • Detection of isoforms of recombinant human erythropoietin by various plant lectins after isoelectric focusing. (tuwien.at)
Detection1
  • The use of analytical isoelectric focusing for detection and identification of beta-lactamases. (nih.gov)
Migration2
  • They were both acidic based on their migration on an acrylamide isoelectric focusing (IEF) gel. (usda.gov)
  • With arginines at both positions, ApoE4 is the most basic of the three species and was designated 4 based on its migration upon isoelectric focusing (see Nomenclature Notes below). (alzforum.org)
Purification1
  • Chromatofocusing purification of CD1b-antigen complexes and their analysis by isoelectric focusing. (illumina.com)
Analysis2
  • Currently his research is focusing on the elimination of organic solvents from the sample preparation step to facilitate on-site monitoring and in-vivo analysis. (uwaterloo.ca)
  • Calf lens homogenates were incubated at temperatures ranging from 37 to 45 degrees-C. Isoelectric focused gel analysis of the lenses incubated for 30 minutes at 45 degrees showed a definite loss in the lowest points of the alpha crystallin components. (cdc.gov)
Molecular2
  • A novel approach to molecular separations is investigated using a technique termed droplet-based isoelectric focusing. (elsevierpure.com)
  • The loss of alpha crystallin from the isoelectric focusing pattern was due to aggregation to higher molecular weight particles which could not enter the gel. (cdc.gov)
Results1