Unlike normal cells, lymphoblasts lack the enzyme to synthesize asparagine and therefore rely on an exogenous source of this amino acid to maintain cellular protein synthesis. (nih.gov)
Contains Asparaginase, kind of enzyme to inhibit protein syntheses by hydrolyzing asparagines (amino acid which is very essentially, needed by cancer/tumor cells) into aspartic acid and ammonia, so the cancer cells/tumor cells will stop growing and die. (iceagefarmer.com)
For example, it is believed that a reaction between free asparagine, an amino acid commonly found in raw vegetables, and free reducing sugars accounts for the majority of acrylamide found in fried food products. (allindianpatents.com)
Lack of asparagine synthetase activity results in cytotoxicity specific for leukemic cells that depend on an exogenous source of the amino acid asparagine. (medscape.com)
Selective killing of leukemic cells it thought to be due to depletion of plasma asparagine, the amino acid required for protein synthesis. (medscape.com)
Asparagine), showing one of the two amino acid substitutions of Haemoglobin C Harlem. (sicklecell.md)
Furthermore, in CRC organoids, which are susceptible to growth suppression by LDN193189, simultaneous treatment with LDN193189 and trametinib, an FDA-approved MEK inhibitor, resulted in cooperative growth inhibition both in vitro and in vivo. (bvsalud.org)
Typhimurium-induced inhibition of T cells in vitro is normally avoided upon addition of L-asparagine. (cell-metabolism.com)
In addition to determining in vitro inhibition abilities of a panel of mAbs against BoNT/B1-/B5, this work has determined B12.2 and 2B18.2 to be the best mAbs for sample preparation before Endopep-MS. These mAb characterizations also have the potential to assist with mechanistic studies of BoNT/B protection and treatment, which is important for studying alternative therapeutics for botulism. (cdc.gov)
15 Dodu SRA, Lewis RA, Konotey-Ahulu FID, Ringelhann B, Boi-Doku FS, Christian EC and Asirifi Y. Inhibition of sickling by phenothiazine: In vitro studies. (sicklecell.md)
Ammonia1
Oncaspar (pegaspargase) is a pegylated version of L-asparaginase, which converts L-asparagine to ammonia and L-aspartic acid, reducing available asparagine resulting in decreased protein synthesis, inhibition of tumor cell proliferation, and increased apoptosis (NCI Drug Dictionary). (jax.org)
Endopeptidase1
Cleavage of tau by asparagine endopeptidase mediates the neurofibrillary pathology in Alzheimer's disease. (istanbul.edu.tr)
Enzyme2
Thus, the inhibition of a single enzyme from the glycolysis or glutaminolysis pathways might not be as efficient therapeutically as dual inhibition of target enzymes. (biomedcentral.com)
Enzyme kinetics of E. coli expressed and purified wild type and N302S TgDHODH revealed an inhibition of the wild type enzyme activity by nanomolar concentrations of 1-hydroxyquinolones, while the IC50s for the N302S mutant were significantly increased. (uni-goettingen.de)
Protein2
Asparagine depletion results in nutritional deprivation, inhibition of protein synthesis, and subsequent apoptotic cell death in lymphoblasts. (nih.gov)
Radiological uptake and competitive inhibition studies with putrescine indicated that rice gene encodes a protein that functioned as a spermidine-preferential transporter. (consensus.app)
Depletion1
Depletion of extracellular asparagine inhibits the growth of lymphocytic leukemic cells. (nih.gov)
Serine2
However, DNA-sequencing identified a single point mutation in codon 302 of the TgDHODH coding sequence that changes a conserved asparagine into serine in the vicinity of the dihydroorotate binding site. (uni-goettingen.de)
Dubois AV , Gauthier A, Bréa D , Varaigne F, Diot P, Gauthier F, Attucci S . Influence of DNA on the activities and inhibition of neutrophil serine proteases in cystic fibrosis sputum. (univ-tours.fr)
Glutamine2
In competition experiments with several amino acids at 25-fold higher levels than spermidine, only methionine, asparagine, and glutamine were effective in reducing uptake of spermidine to 60% of control rates. (consensus.app)
Henceforth, we aimed to investigate if dual inhibition of these pathways by phytobiological compound c28MS has the potential of synergistic therapy for arthritis by targeting both glucose and glutamine metabolism. (biomedcentral.com)
Inhibits1
This agent inhibits dihydrofolate reductase, resulting in inhibition of DNA synthesis, repair, and cellular replication. (medscape.com)
Pathways2
PA improve the inhibition of hematopoietic function in myelosuppression mouse, and its mechanisms may be related to anti-oxidation and promoting the expression of hematopoietic-related cytokines and regulating the related metabolic pathways. (bvsalud.org)
To distinguish whether the lack of TgDHODH-mediated electron transfer or the inhibition of de novo pyrimidine biosynthesis is responsible for growth inhibition, both pathways were uncoupled by expression of the ubiquinone independent Saccharomyces cerevisiae DHODH (ScDHODH). (uni-goettingen.de)
Cleavage1
This enzymatic cleavage results in the inhibition of the nerve impulse, leading to flaccid paralysis of the victim which can affect the lungs and may necessitate ventilator support. (cdc.gov)
Knockdown1
Chemical inhibition or RNAi-mediated knockdown of FIH promotes LCV-lysosomes fusion, diminishes decoration of the LCV with polyubiquitinated proteins, and abolishes intra-vacuolar replication of L. pneumophila. (huji.ac.il)
Alzheimer's1
Review on Alzheimer's disease: Inhibition of amyloid beta and tau tangle formation. (istanbul.edu.tr)
Findings3
Our findings imply that dual inhibition of glycolysis and glutaminolysis could be an effective approach for the treatment of RA. (biomedcentral.com)
Evaluation of the effects of the isoeugenol on AChE, α-amylase, and α-glycosidase was an important aim of this article and inhibition findings are represented in Table 4. (researchgate.net)
Catalytic1
We find that noncatalytic inhibition follows the same activity dependence pattern as catalytic modulation (phosphorylation): the efficacy and apparent affinity of genistein inhibition are much higher for closed than for fully activated channels. (silverchair.com)
Proliferation1
PANX1 channel inhibition using probenecid (PBN) or carbenoxolone (CBX) reduced the proliferation of our panel of high-risk NB cell lines. (jcancer.org)
Compounds1
Furthermore, Plasmodium GGPP synthase (GGPPS) can bind nitrogen-containing bisphosphonates (N-BPs) strongly with the energetically favorable cooperation of three Mg(2+), resulting in inhibition by this class of compounds at IC(50) concentrations below 100 nM. (ox.ac.uk)
Cysteine1
In silico binding study of compound 5d with plasmodial cysteine protease falcipain-2 indicated the inhibition of falcipain-2 as the probable reason for the antimalarial potency of compound 5d . (biomedcentral.com)
Pharmacological1
2010). Pharmacological effects of Catharanthus roseus root alkaloids in acetylcholinesterase inhibition and cholinergic neurotransmission . (up.pt)
Gene1
Among 18 molecularly characterized CRC organoids, suppression of growth by BMP inhibition correlated with induction of LRIG1 gene expression. (bvsalud.org)
Growth3
Anti-cancer: Inhibition effect on growth of cancer cells. (iceagefarmer.com)
Heterologous expression of the 1-hydroxyquinolone-insensitive ScDHODH in T. gondii should lead to a restoration of de novo pyrimidine biosynthesis in the presence of HDQ and compound B. These parasites showed a strongly increased resistance towards 1-hydroxyquinolones, demonstrating that inhibition of pyrimidine de novo synthesis significantly contributes to the growth inhibitory potential of 1-hydroxyquinolones in T. gondii. (uni-goettingen.de)
The growth kinetics of drug treated parasites is characterized by a biphasic pattern, with an initial phase (~ 0 to 24 hours) in which the parasites - independent of their resistance or sensitivity to 1-hydroxyquinolones - show an almost complete growth inhibition. (uni-goettingen.de)
Survival1
2016). Leishmania infantum Asparagine Synthetase A Is Dispensable for Parasites Survival and Infectivity . (up.pt)
Results1
These results were interpreted as highly reduced inhibition by zanamivir, normal inhibition by oseltamivir, and reduced inhibition by peramivir and laninamivir ( Table 1 ) ( 5 ). (cdc.gov)
Studies1
Studies on chimeric channels containing portions of RETα, which exhibits genistein inhibition, and the rat olfactory CNG channel α-subunit, which does not, reveals that a domain containing S6 and flanking regions is the crucial for genistein inhibition and may constitute the genistein-PTK binding site. (silverchair.com)
Activity1
Based on the fact that seizure activity is frequently associated with autism and that abnormal evoked potentials have been observed in autistic individuals in response to tasks that require attention, several investigators have recently proposed that autism might be caused by an imbalance between excitation and inhibition in key neural systems including the cortex. (neurotransmitter.net)
Channel1
Ni 2+ , which potentiates RETα channel gating, partially relieves genistein inhibition, possibly by disrupting the association between the genistein-PTK and the channel. (silverchair.com)
Competitive1
Furthermore, inhibition kinetics demonstrated that 1-hydroxyquinolones act as competitive inhibitors for the electron acceptor QD, but as uncompetitive inhibitors for dihydroorotate. (uni-goettingen.de)
Specific2
Among those, several candidate genes have been shown to control the early patterning and/or the late synaptic maturation of specific neuronal subpopulations controlling the balance between excitation and inhibition in the developing cortex and cerebellum. (neurotransmitter.net)
Half maximal inhibition concentration (IC 50 ) is a measure of the potency of a substance in inhibiting a specific biological or biochemical function. (researchgate.net)