Indirect biological assay. Medical search. Frequent questions

Anti-DFS70 autoantibody determination using enzyme-linked immunosorbent assay (ELISA) testing of IgG autoantibodies to human DFS70 antigen was performed by a DFS70 ELISA kit from Medical & Biological Laboratories Co., LTD (7808, Japan) as per manufacturer's instructions. (cdc.gov)
Serum samples previously tested positive in HEp-2 indirect immunofluorescence assay (IFA) with nuclear dense fine speckled pattern (AC-2) were selected for anti-DFS70 autoantibody ELISA analyses. (cdc.gov)
Mouse NGF/proNGF Neutralizing Antibody (#ALM-006) can be used in indirect ELISA and neutralization assays to block the biological effects of both NGF and proNGF. (alomone.com)
The Avidin-Biotin system has been utilized extensively for enzyme-linked immuno solid-phase assay (ELISA), in which an enzyme-Avidin conjugate (useful for detection by reaction with the enzyme's substrate to afford a colored or chemiluminescent product) is employed to detect the presence of a biotinylated antibody, after first binding the antibody to an immobilized antigen or hapten. (justia.com)
An indirect ELISA and a real time immuno-PCR (RT-iPCR) assay for the timely and sensitive detection of A. euteiches were developed using antiserum specific to oospores of A. euteiches isolates. (uleth.ca)
RT-iPCR was 100 times more sensitive over the linear working range than indirect ELISA. (uleth.ca)
ELISA kits are assay systems that utilize antibodies, enzymes, and color-changing substrates to detect and quantify specific molecules in biological samples. (rajbiosis.com)
ELISA kits typically include pre-coated microplates, reagents, and detailed protocols for performing specific assays. (rajbiosis.com)
We offer FRET and Fluorogenic detection-based assays , as well as ELISA kits all categorized by research or disease area. (synchrone.studio)
The selectivity for a specific protease activity is enhanced by combining the specificity of an ELISA, with the sensitivity of FRET into a single assay. (synchrone.studio)
ELISA (Enzyme-linked immunosorbent assay) is an immunological assay for the measurement of antibodies, proteins, and antigens in biological samples. (synchrone.studio)
There are several variations of ELISA assays but all utilize a coating of either antibody or antigen to capture and immobilize the molecule of interest onto the assay plate. (synchrone.studio)
In the indirect and sandwich ELISA methods, an unlabeled primary antibody binds the already immobilized molecule of interest, followed by a labeled secondary antibody that recognizes the primary antibody. (synchrone.studio)
Enzyme-linked immunosorbent assays (ELISA) have been around as one of the primary methods of analyte detection for more than four decades. (ddw-online.com)
Over the years many changes to the basic format have resulted in assay improvements, but some of the most recent look set to take the traditional ELISA to new levels. (ddw-online.com)
These include many vendors now addressing the automation of ELISA assays. (ddw-online.com)
Other automated instrumentation is designed to support the creation/coating of ELISA assay plates or contains cartridge-based delivery of assay reagents to reduce set-up time. (ddw-online.com)
These are supported by a wide range of ELISA reagents, assay and kits providing relative profiling or absolute quantitation of proteins across a wide range of study areas. (ddw-online.com)
E nzyme-linked immunosorbent assays (ELISA) have been one of the primary methods of detecting the presence of a substance, usually an antigen, in an aqueous sample for more than 40 years. (ddw-online.com)
However, numerous variants of this format have been developed over the years (eg indirect, sandwich, competitive, etc) and a multitude of detection chemistries have been applied to ELISA, including chemiluminescence and various fluorescent readouts. (ddw-online.com)
The immunoassay landscape has, however, dramatically changed in recent years with the development of reduced assay steps protocols, no wash ELISAs and new automated systems designed to run ELISA. (ddw-online.com)
This review article uses as its starting point some of the findings of HTStec's ELISA Assay Trends 2012 survey and report, published in June 2012 (1). (ddw-online.com)
This report discusses end-users' current practices and preferences in ELISA assays, and seeks to understand future user requirements. (ddw-online.com)
The main (primary) application area for survey respondents' ELISA assays was immunology (17% selecting). (ddw-online.com)
RÉSUMÉ L'objectif de cette étude réalisée en Iraq était de définir la sensibilité et la spécificité d'un test ELISA commercial pour la détection de l'antigène de Giardia lamblia dans les selles. (who.int)

MRFR is the Leading Brand in The Research Company who Recently Published Immunofluorescence Assay Market Research Reports which includes Study of growth, Regional Analysis, Top Industry Players Formation, Major Drivers, Upcoming Trends and Forecast to 2023. (medgadget.com)
Also, growing demand for rapid and precise measurement of biomolecules, increased use of Immunofluorescence Assay for diagnostic and research purposes, increased R&D activities and advanced technology are the key factors for the market to grow. (medgadget.com)
The global immunofluorescence assay market is segmented on the basis of technique, product type, application and end user. (medgadget.com)
The immunofluorescence assay market, by technique is sub segmented into primary (direct) immunofluorescence assays, secondary (indirect) immunofluorescence assays, and micro immunofluorescence. (medgadget.com)
The Immunofluorescence Assay market is dominated by North America owing to the high prevalence of chronic diseases and high R&D investments within this region stimulate market growth. (medgadget.com)
It is estimated that Europe stood second in the global Immunofluorescence Assay market. (medgadget.com)
Anti-HMGCR positivity could be associated to a cytoplasmic pattern in immunofluorescence assay on HEp-2 cells. (biomedcentral.com)

It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. (aatbio.com)
Based on indirect immunofluorescent antibody assays, the fibronectin deposited from clotting plasma appeared to be in a punctate distribution over the entire material surface and occasionally was associated with discrete fibrillar structures. (elsevierpure.com)

The role of potency assays will also be addressed. (frontiersin.org)
Biological weapons are often characterized by low visibility, high potency, substantial accessibility, and relatively easy delivery. (medscape.com)
By Ames assay, the relative mutagenic potency ratios of methyl-chloride, methyl-bromide, and methyl-iodide are 1 to 40 to 10. (cdc.gov)
Effective use of hierarchical lines of evidence relies on consideration of the rele- vance and potency of assays, organization of assays by mode of action, and better assay validation. (cdc.gov)

detection of gene probes, enzyme-linked immuno solid-phase assay, monoclonal antibody drug targeting and medical imaging. (justia.com)
In this type of assay, a monoclonal antibody is employed to first pull out the specific protease of interest, before incubation with the protease substrate. (synchrone.studio)

We will comprehensively evaluate the required assay format, cell line, NAbs, optimize assay parameters and processing/pre-processing procedures to offer customized NAbs assay development services with our cutting-edge recombinant antibody production platform , and provide optimized standardized NAbs functional evaluations. (creative-biolabs.com)

Most of the assay methods for quantifying uromodulin rely on the availability of antibodies to this glycoprotein. (tubitak.gov.tr)
Al- direct demonstration or cultivation of the though a number of papers have reported causative organism, remain the definitive the existence of circulating antigens and laboratory diagnostic method for VL, sero- immune complexes in VL [ 16-19 ], none of diagnostic methods are needed to over- them has led to a useful antigen detection come the diagnostic difficulties assay for VL. (who.int)
Most methods used for NO detection are indirect including spectroscopic approaches such as the Griess assay for nitrite and detection of methemoglobin after NO reaction with oxyhemoglobin. (intechopen.com)
Therefore, direct methods are necessary for investigating biological process and diseases related to NO in biological conditions. (intechopen.com)
Although numerous methods of indirect bioconjugate preparation have been described, a significant number of those reported in the literature have been prepared by exploiting the Avidin-Biotin system, in which, the binding specificity of the protein Avidin (purified from egg white), or Streptavidin (purified from the bacterium Streptomyces avidinii), toward the cofactor Biotin (vitamin H) is utilized to bridge an Avidin conjugated macromolecule with a biotinylated macromolecule. (justia.com)
Today, many different serological test methods for the indirect detection of bacteria and viruses are established in routine microbiological laboratories. (instand-ev.de)
Since then, the detection of pathogen-specific antibodies by immunological methods has remained an essential pillar for diagnosing pathogens from blood and CSF, despite the significant progress of modern molecular biological assays for direct pathogen detection. (instand-ev.de)
The indirect diagnosis of bacterial pathogens by serological assays has the advantage that an immune response can also be detected against microorganisms that are difficult to cultivate or slow-growing and can hardly be detected by culture or molecular methods. (instand-ev.de)
Methods for the determination of formaldehyde in biological samples are given in Table 6-1. (cdc.gov)
If the MoA of the drug or biopharmaceutical is blocking soluble targets/cell receptors or if developing reliable cell-based assays is challenging, non-cell-based detection methods are considered appropriate. (creative-biolabs.com)

The present invention relates to the field of bioconjugate preparation, and more particularly, to a class of bifunctional boronic compound complexing reagents useful for the conjugation of biological macromolecules, and the method of making and using such reagents. (justia.com)

Based on this method, we performed cytotoxicity assays on 42 AsPCs grown both as monolayers and spheroids. (biomedcentral.com)

These prob- mania antibodies have been described, lems could, theoretically, be avoided by de- including indirect immunofluorescence tecting antigen in the urine [ 20-22 ]. (who.int)
Affinity-purified GST or GST-PARP1 used in this assay is usually shown in the lower panel following blotting using anti-PARP1 and anti-GST antibodies. (scienza-under-18.org)
3 Two stage screening included a sensitive initial radioimmunoassay for antibodies to tissue transglutaminase (endomysial antigen) with further testing of positive samples for IgA-EMA by indirect immunofluorescence. (bmj.com)
The development of Neutralizing Antibodies (NAbs) detection can provide effective immunological information for biopharmaceuticals or related disease intervention strategies, and the specific assay format selection depends on a combination of three key factors: drug type and mechanism of action (MoA), immunogenicity risk, and assay performance in obtaining reliable data. (creative-biolabs.com)

AnaSpec's SensoLyte FRET assay kits feature peptide substrates labeled with our proprietary long wavelength FRET pairs for higher sensitivity . (synchrone.studio)
We have developed a 3D (spheroid) functional assay to assess the sensitivity of two PARPis (niraparib and olaparib) in ascites-derived primary cell cultures (AsPCs) from HGSOC patients. (biomedcentral.com)
P erhaps most significantly are the big increases in assay sensitivity and the widening of the dynamic range accomplished by using alternatives to the traditional colorimetric detection, or by the use of new platform technologies. (ddw-online.com)

The chemopreventive activity of these berries and their fractions was evaluated by the determination of their antioxidant activity using a panel of in vitro tests (DPPH, ABTS) and their antimutagenic properties against dietary and environmental, direct and indirect, mutagens. (unimore.it)
The Nitric Oxide Detection Kit is a colorimetric assay for the indirect determination of Nitric Oxide concentrations based on the enzymatic conversion of nitrate to nitrite by a nitrate reductase. (ozbiosciences.com)

: Global Industry Trends, Share, Size, Growth, Opportunity and Forecast 2022-2027, " The global lateral flow assay market size reached US$ 9.3 Billion in 2021. (upfuture.net)
Lateral flow assay (LFA), or lateral flow immunochromatographic assay, is a paper-based testing solution used in laboratories to detect analytes in complex mixtures. (upfuture.net)
The increasing prevalence of chronic and infectious diseases primarily drives the global lateral flow assay market. (upfuture.net)

These results illustrate the combined impact of genetic, lifestyle, and environmental factors in modulating immunological responsivity and emphasize the importance of the exposome in shaping biological responses. (cdc.gov)

This assay is based on the reaction originally described by Griess to detect NO 2 - in a variety of biological fluids such as cell culture medium, plasma, serum, or urine. (ozbiosciences.com)

A multiplex assay (ALBIA-NAM) was also developed to permit the simultaneous quantification of anti-HMGCR and anti-signal recognition particle autoantibodies. (biomedcentral.com)

Bioconjugation is a descriptive term for the joining of two or more different molecular species by chemical or biological means, in which at least one of the molecular species is a biological macromolecule. (justia.com)
Indirect measurements, analyze the assessment of parameters that are linked to the biomechanical properties of the skin and give information on: Collagen, Face analysis and volume, molecular composition, proteomics and metagenomics, dermis size and shape, Skin aspect and structure. (premiumbeautynews.com)

The history of protein dynamics traces back to 1935, when Schoenheimer and Rittenberg synthesized the first isotopologs of biological molecules to demonstrate the continuous renewal of proteins throughout life 14 , 15 . (nature.com)
Depending on the selected signaling molecules for the detection endpoint, non-cell-based NAbs assays can also be further classified (Ligand Binding NAb Assays, Enzymatic Activity NAb Assays, etc . (creative-biolabs.com)
In the biological sciences the suffix -omics is used to refer to the study of large sets of biological molecules. (who.int)
Through various omic technologies it is now possible to interrogate large sets of biological molecules. (who.int)

This technique is a reliable method for the titration of human coronaviruses (HCoV) in biological samples (cells, tissues, or fluids). (wikipedia.org)
It was noted by the authors that this method was free from interferences from proteins and bacterial cells so it might have applicability to biological fluids such as blood or urine. (cdc.gov)
Serum, plasma and other biological fluids. (genetaq.com)

Bioconjugates are generally classified as either direct or indirect conjugates. (justia.com)
Alternatively, indirect conjugates encompass those in which two or more components are joined via an intermediary complex involving a biological macromolecule. (justia.com)
The system described herein is the first to enable the formation of indirect conjugates without dependence upon an intermediary biological macromolecule. (justia.com)

For the other two compounds, little usable data is available, and the magnitude of human risk associated with these compounds is based on biological activities and likely mechanisms of action. (cdc.gov)

The application of this assay and the potential biomarkers with possible predictive significance to PARPis therapy of EOC patients now need testing in the setting of a clinical trial. (biomedcentral.com)

Biological weapons include any organism or toxin found in nature that can be used to incapacitate, kill, or otherwise impede an adversary. (medscape.com)

Phenotypic traits such as Gram stain, colony shape, growth on various nutrient mediums, and other biochemical assays are the basis for identification and classification in clinical laboratories. (azooptics.com)

Accordingly, understanding the underlying mechanism of cellular uptake is an important step toward understanding the biological fate of nanoparticles, both the favorable and adverse aspects [1] . (materialstoday.com)
Grinnell, F & Phan, TV 1983, ' Deposition of fibronectin on material surfaces exposed to plasma: Quantitative and biological studies ', Journal of cellular physiology , vol. 116, no. 3, pp. 289-296. (elsevierpure.com)

Based on whether the drug directly acts on cells or stimulates a response, cell-based analyses can be further classified as direct or indirect detection. (creative-biolabs.com)

The results of these biological assays were correlated with the chemical composition of the two investigated Vaccinium species, to establish the potential application of V. floribundum as a source of phytochemicals in the nutraceutical and functional food ambit. (unimore.it)
Cell-based analyses provide cell line-specific functional measurements, which can more accurately describe the overall physiological effects of NAbs in biological systems rather than limited information on NAbs-drug interactions. (creative-biolabs.com)

Indirect immunoperoxidase assay (IPA) is a laboratory technique used to detect and titrate viruses that do not cause measurable cytopathic effects and cannot be measured by classical plaque assays. (wikipedia.org)
The Influenza viruses form a virus group with principally similar morphological, chemical and biological features. (rapidtest.com)

The present approach provides an easy and reliable means for simultaneous and defectless passivation and submicrometer patterning of proteins, thus indicating its potential application in biological assay. (nus.edu.sg)
Colony development assay HIPK2 expression vector (pCMV-Flag) was transfected into HCT116 cells, with or without PARP1 expression vector for 24?h. of HIPK2 by regulating the proteins balance of Ethopabate HIPK2. (scienza-under-18.org)

An extraction protocol was developed by spiking the soils with A. euteiches oospores and the RT-iPCR assay was used to quantify oospores in the soil extracts. (uleth.ca)

the understanding of MSC biology, their mechanism of action (MoA) in different biological contexts, and their targeted and routine use in the clinics is limited ( Hoogduijn and Lombardo, 2019 ). (frontiersin.org)

The global Immunofluorescence assays market is expected to reach an approximate CAGR of 5.8% during the forecast period. (medgadget.com)

Secondary (indirect) immunofluorescence assays category includes salt split technique, antigenic mapping method and double staining method. (medgadget.com)
Here, we report an indirect soft-lithography-mediated method of fabricating hemoglobin (Hb) patterns in the submicrometer length scales region on indium-tin-oxide (ITO). (nus.edu.sg)
This assay method is the basis for our SensoLyte® Plus MMP-9 and SensoLyte ® Plus MMP-13 Plus assay kits . (synchrone.studio)
Briefly, this decision confirmed that an act of direct infringement must be proven before there can be a finding of indirect infringement and that direct infringement requires all the steps of the claimed method to be performed by, or attributable to, a single entity. (mintz.com)
So in addition to proof of direct infringement, a theory of indirect infringement is necessary to get at the target defendant, typically the entity performing the "assaying" step of the claimed method. (mintz.com)

It also assists in producing simple, rapid, cost-effective, and portable detection devices that are utilized for testing biological samples of serum, plasma, and blood. (upfuture.net)
When compared to air sampled prior to ignition, samples of the air-smoke mixtures contained fivefold higher concentrations of microbial cells (6.7 ± 1.3 × 10 4 cells m −3 ) and biological INPs (2.4 ± 0.91 × 10 3 INPs m −3 active at temperatures ≥ −15 °C), and these data significantly positively correlated with PM 10 . (nature.com)
Of 84 stool samples from children in Duhok governorate, 42 were positive and 42 negative for G. lamblia or other parasites by direct and indirect microscopic examination. (who.int)

Biological activity (fibronectin‐promoted cell spreading) was also greatest at low plasma concentrations and decreased as the plasma concentration was raised. (elsevierpure.com)

The German-American physician Anton Dilger established a secret biological laboratory in Chevy Chase, Maryland, with the intent to grow the causative agents of anthrax and glanders. (medscape.com)

To assess the performance of the RT-iPCR assay, non-infested soils representing a range of soil textures were collected from Alberta fields. (uleth.ca)

Since 2004, AnaSpec has manufactured assay kits for life scientists around the world and we continuously develop new kits , all with accuracy , speed and convenience in mind. (synchrone.studio)

College of Environmental and Biological Technology, Hengyang 421005,China;3. (radaris.asia)
Despite the varied roles of bioaerosols in environmental health, biological dispersion, and the land-atmosphere system, their ecological sources and emission mechanisms remain poorly understood [ 13 ]. (nature.com)

assay,enzymeimmunoassay,counter- Microscopy immunoelectrophoresis and radio- Thesampleswereprocessedimmedi- Analysis immune precipitation assay. (who.int)

Therefore, the accurate and reliable measurement of physiological NO concentration is essential to the understanding of NO signaling and its biological role. (intechopen.com)

Anti-HMGCR levels were assayed in 150 patients with suspicion of NAM, 142 controls with different inflammatory/autoimmune diseases and 100 healthy donors. (biomedcentral.com)

KAtex est un simple supplément pour le diagnostic de la leishmaniose viscérale, en particulier sur le terrain, et un test complémentaire pour le diagnostic de la leishmaniose viscérale dans les cas à frottis négatif ayant des résultats positifs au test d'agglutination directe. (who.int)
Nous recommandons l'utilisation de la méthode immunoenzymatique dans les études épidémiologiques en Iraq, ainsi que pour confirmer le diagnostic chez les patients présentant des symptômes cliniques caractéristiques de la giardiase mais dont les résultats par microscopie directe sont négatifs. (who.int)

The diversity of proteoglycans and their high interaction with growth factors and their receptors provides structural basis for a multitude of biological functions. (premiumbeautynews.com)

Their application has become feasible in recent years due to an increase in the resolution and throughput of omics-based assays along with a lowering of cost. (who.int)

The cell experiments via an indirect contact assay demonstrated that these "tetrazole?alkene" photo-click PEG hydrogels were noncytotoxic. (polymer.cn)
Cell-based assay (CBA) and competitive ligand-binding assay (CLBA). (creative-biolabs.com)

Under the current state of US law, in order to clear the subject matter eligibility hurdle, these types of claims will typically need to combine several discrete steps such as "obtaining" a biological sample from a patient, "assaying" the sample for a biomarker, and "administering" a therapeutic agent to the patient based on the assay results. (mintz.com)

This has generated very significant interest in assessing the biological effects of proton pulses delivered at very high dose rates. (frontiersin.org)
This increase could reflect undercounting COVID-19 deaths or indirect effects of the virus, such as underutilization of, or stresses on, the health care system (8). (cdc.gov)

Bioconjugation is utilized extensively in biochemical, immunochemical and molecuar biological research. (justia.com)

When surfaces were exposed to plasma under clotting conditions (i.e., addition of Ca 2+ and thrombin), fibronectin deposition on the surfaces and biological activity remained constant or increased as the plasma concentration was raised. (elsevierpure.com)
On the other hand, the increase in biological activity that occurred if the surfaces were exposed to clotting plasma was completely inhibited by putrescine, indicating that fibronectin had to be crosslinked to fibrin to have biological activity under these conditions. (elsevierpure.com)

This was the first multilateral agreement that extended prohibition of chemical agents to biological agents. (medscape.com)

Anatomy3

Organisms2

Chemicals and Drugs6

Analytical, Diagnostic and Therapeutic Techniques and Equipment8

Phenomena and Processes12

Anthropology, Education, Sociology and Social Phenomena1

Information Science4

ELISA25

Immunofluorescence assay7

Immunofluorescent2

Potency4

Monoclonal antibody2

Antibody1

Methods10

Reagents1

Cytotoxicity1

Antibodies4

Sensitivity3

Determination2

Lateral flow3

Immunological1

Griess1

Autoantibodies1

Molecular2

Molecules4

Fluids3

Conjugates3

Mechanisms of act1

Biomarkers1

Organism1

Clinical1

Cellular2

Direct or indirect1

Functional2

Viruses2

Proteins2

Quantify1

Mechanism of act1

CAGR1

Method5

Samples3

Concentrations1

Laboratory1

Assess1

Kits1

Environmental2

Immune1

Measurement1

Diseases1

Diagnostic2

Receptors1

Application1

Cell2

Results1

Effects2

Biochemical1

Activity2

Chemical1