Inactivation spore bacillus cereus. Medical search. Frequent questions

or as six species in a Bacillus cereus sensu lato: B. weihenstephanensis, B. mycoides, B. pseudomycoides, B. cereus, B. thuringiensis, and B. anthracis. (wikipedia.org)
A phylogenomic analysis combined with average nucleotide identity (ANI) analysis revealed that the B. anthracis species also includes strains annotated as B. cereus and B. thuringiensis. (wikipedia.org)
Inactivation of Vegetative Cells, but Not Spores, of Bacillus anthracis, B. cereus, and B. subtilis on Stainless Steel Surfaces Coated with an Antimicrobial Silver- and Zinc-Containing Zeolite Formulation. (biophysica.com)
Spores of Bacillus anthracis , the agent that causes anthrax, are one of the microorganisms most refractory to inactivation by the boiling water method. (cdc.gov)
This study was conducted to determine the resistance of spores of B . anthracis Sterne and three other strains of Bacillus spp. (cdc.gov)
In a comprehensive literature review citing published reports dating back to 1882, Murray ( 8 ) noted that boiling times reported to destroy B . anthracis spores varied over a range of 1 to 12 min. (cdc.gov)
In his own study of 17 strains of B . anthracis , Murray ( 8 ) found that boiling times of 5 to 10 min were required to achieve inactivation. (cdc.gov)
Stein and Rogers ( 9 ) reported that vigorous boiling for 3 to 5 min destroyed spores from 43 strains of B. anthracis . (cdc.gov)
Bacillus anthracis is a spore-forming microbe that persists in soil and causes anthrax disease. (bvsalud.org)

B. cereus bacteria may be anaerobes or facultative anaerobes, and like other members of the genus Bacillus, can produce protective endospores. (wikipedia.org)
These bacteria are both spore-forming and biofilm-forming, presenting a large challenge to the food industry due to their contamination capability. (wikipedia.org)
B. cereus display flagellar motility, which has been shown to aid in biofilm formation via an increased ability to reach surfaces suitable for biofilm formation, to spread the biofilm over a larger surface area, and to recruit planktonic, or single, free-living bacteria. (wikipedia.org)
This novel method and the unique properties of the generated EWNS could potentially be used to develop an effective, environmentally friendly, and inexpensive method for bacteria inactivation. (springer.com)
The objective of this study was to evaluate the log reductions and thermal inactivation kinetics of spores of Bacillus subtilis bacteria and ascospores of Talaromyces flavus and Eupenicillium javanicum molds under influence of pH and sodium benzoate preservative. (hindawi.com)
Contamination and isolation of molds such as Talaromyces flavus and Eupenicillium javanicum and bacteria such as Bacillus subtilis in juices have been reported in past literatures [ 6 - 10 ]. (hindawi.com)
There is a growing need to be able to detect and identify spore forming bacteria. (justia.com)
These wavelengths are known for their ability to eliminate 99.9% of pathogens, such as viruses, bacteria, fungi, and mold spores. (white-coat.org)
Bacillus is a genus of gram-positive, rod-shaped bacteria and a member of the phylum Firmicutes . (fortunejournals.com)
Under stressful environmental conditions, the bacteria can produce oval endospores that are not true 'spores', but to which the bacteria can reduce themselves and remain in a dormant state for very long periods. (fortunejournals.com)

9 Revival in formaldehyde-treated Bacillus subtilis spores was increased by up to 1 log using heat shock for 30 minutes before plating out. (basicmedicalkey.com)

This study discussed the effects of different bacterial concentrations and centrifugations on the antimicrobial efficacy of electrolyzed oxidizing (EO) water on Bacillus subtilis and Escherichia coli O157:H7. (hocl.com)
such as Bacillus subtilis , is also one of the predominant microbes from bacterial genera found in soil and may also be introduced into the manufacturing process through poorly washed fruits. (hindawi.com)
Because of their ubiquity and resistance to space craft decontamination, bacterial spores are considered likely potential forward contaminants on robotic missions to mars (Nicholson, et al. (com.ng)
For example in the food industry there is a desire for rapid analysis of food stuffs to detect the presence of any bacterial spores, such as Bacillus cereus , before they can germinate and spoil produce and/or cause illness. (justia.com)
Any remaining bacterial spores are generally unable to germinate due to the acidic nature of the juices [ 1 ]. (biomedcentral.com)

Gram-negative Serratia marcescens , gram-positive Staphylococcus aureus , and Bacillus atrophaeus endospores were placed on stainless steel coupons and exposed to generated EWNS at multiple time intervals. (springer.com)
Chen CY, Wu LC, Chen HY, Chung YC (2010) Inactivation of Staphylococcus aureus and Escherichia coli in water using photocatalysis with fixed TiO 2 . (springer.com)

Because transmission to new hosts depends on the release of large numbers of spores in the environment, we propose that the acquisition of pXO1- and pXO2-like plasmids may promote the successful expansion of members of the Bacillus cereus sensu lato group able to cause anthrax-like disease. (bvsalud.org)

Bacillus cereus is a Gram-positive rod-shaped bacterium commonly found in soil, food, and marine sponges. (wikipedia.org)
B. cereus is a rod-shaped bacterium with a Gram-positive cell envelope. (wikipedia.org)
No other reports were reported for this bacterium spores in high-acid foods. (hindawi.com)
Bacillus cereus is a spore-forming bacterium that can cause foodborne diseases. (clordisys.com)

Some strains are harmful to humans and cause foodborne illness due to their spore-forming nature, while other strains can be beneficial as probiotics for animals, and even exhibit mutualism with certain plants. (wikipedia.org)
B. cereus strains exhibit flagellar motility. (wikipedia.org)
Spores of one of the B . cereus strains were obtained from a commercial source (Raven Biological Laboratories, Omaha, NE). (cdc.gov)
Spores of all strains of the Bacillus spp. (cdc.gov)
SIGNIFICANCE: The emetic strains of Bacillus cereus are known to cause severe food poisoning, making it crucial to understand the factors contributing to their selective enrichment in foods. (bvsalud.org)
The antibiotic activity of the essential oil was assessed on 05 strains of Bacillus ( B. amyloliquefaciens FZB42, B. amyloliquefaciens S499, B. subtilis ATCC 21332, B. licheniformis ATCC 14580, B. pumilus ), using the method of diffusion in a solid medium. (fortunejournals.com)
The essential oil of Origanum vulgare proved to be endowed with bactericidal properties against Bacillus strains. (fortunejournals.com)

Spores of some Bacillus species are responsible for food spoilage and foodborne disease. (hocl.com)
Gilbert R, Turnbull P, Parry J, Kramer J (1981) Bacillus cereus and other bacillus species: their part in food poisoning and other clinical infections. (springer.com)
However the method is not good at discriminating between different Bacillus species as the origin and identity of the biomarkers may be unclear and different species may have similar biomarkers. (justia.com)
Bacillus species can be obligate aerobes (oxygen reliant), or facultative anaerobes (having the ability to be aerobic or anaerobic). (fortunejournals.com)
Ubiquitous in nature, Bacillus includes both free-living (nonparasitic) and parasitic pathogenic species. (fortunejournals.com)

We find that spores germinate in the GI tract and precipitate disease in a dose-dependent manner. (bvsalud.org)

Bacillus cereus is a gram-positive, facultatively aerobic spore-former whose cells are large rods and spores do not swell the sporangium. (environsafety.com)
Bacillus cereus is a food-borne Gram-positive pathogen. (bvsalud.org)

In this regard, representatives of the genus Bacillus are known as producers of many enzymes and antimicrobial compounds. (encyclopedia.pub)

Inoculation of vegetative bacilli also results in GI anthrax. (bvsalud.org)
After host infection by C. difﬁcile spores, germination occurs, and the vegetative forms multiply in the gut. (studyres.com)

Bacillus cereus has mechanisms for both aerobic and anaerobic respiration, making it a facultative anaerobe. (wikipedia.org)

On the basis of the initial levels of spores used in this study, holding water at a rolling boil for 1-3 min in an open container would not inactivate the spores. (cdc.gov)
A combined hurdle approach of slightly acidic electrolyzed water simultaneous with ultrasound to inactivate Bacillus cereus on potato. (hocl.com)

Biofilm formation may also occur while in spore form due to varying adhesion ability of spores. (wikipedia.org)
This team evaluated chlorine dioxide's ability to kill Bacillus cereus spores in biofilm formed on a stainless steel surface. (clordisys.com)

The current gold standard for detection of spores is germination via heat activation and outgrowth. (justia.com)
The pathology process of C. difﬁcile is divided in three main steps as follows: spore germination, host colonization and toxin production. (studyres.com)

The second B . cereus (ATCC 9592) was grown in a generic sporulation medium ( 5 ), and B . thuringiensis var. (cdc.gov)
The aim of this study was to establish whether this protein is involved in the D -alanylation process in Bacillus thuringiensis . (frontiersin.org)

However, when the spore has a silica coating, it may reduce the permeability of the membrane and provide resistance to many acids. (wikipedia.org)
however, if there are known strain-to-strain variations in the resistance of a select agent to an inactivation procedure, then an inactivation procedure validated on a lesser resistant strain must also be validated on the more resistant strains. (lawstack.com)

Kansas State University completed preliminary testing on RGF's Photohydroionization® (PHI-Cell®) and Reflective Electromagnetic Energy (REME® Cell) technologies with 99+% inactivation of H1N1 Swine Flu on a stainless steel surface. (environsafety.com)

The B. cereus genome encodes genes for metabolic enzymes including NADH dehydrogenases, succinate dehydrogenase, complex III, and cytochrome c oxidase, as well as others. (wikipedia.org)
A genome analysis identified two genes encoding the S-layer proteins SL2 and EA1, which we experimentally confirmed to encode the two protein components of the S-layer covering the surface of B. cereus. (bvsalud.org)

5) Material containing a select agent that is subjected to a procedure that removes all viable select agent cells, spores, or virus particles if the material is subjected to a viability testing protocol to ensure that the removal method has rendered the material free of all viable select agent. (lawstack.com)
6) A select agent or regulated nucleic acids that can produce infectious forms of any select agent virus not subjected to a validated inactivation procedure or material containing a select agent not subjected to a procedure that removes all viable select agent cells, spores, or virus particles if the material is determined by the HHS Secretary to be effectively inactivated or effectively removed. (lawstack.com)

Some studies and observations have shown that silica particles the size of a few nanometers have been deposited in a spore coat layer in the extracytoplasmic region of the Bacillus cereus spore. (wikipedia.org)
11 Complete inactivation depends on the physical state of the particles. (basicmedicalkey.com)

The Embden-Meyerhof pathway is the predominant pathway used by Bacillus cereus to catabolize glucose at every stage of the cell's development, according to estimates of a radiorespirometric method of glucose catabolism. (wikipedia.org)

Nondetectable level of B. cereus spore in suspension occurred within 2 min after exposure to both EO waters containing 120 mg/L ACC, while only SAEW at 120 mg/L and 2 min treatment achieved >6 log reductions of B. subtilis spore. (hocl.com)
EO water with ACC of 80 mg/L and treatment time of 3 min on carrier test without organics addition resulted in reductions of B. subtilis spore to nondetectable level. (hocl.com)

MALDI has been used to detect specific biomarkers associated with the outer layers of the spores. (justia.com)

Therefore, the aim of this study was to develop a hurdle approach that combined SAcEW and US to improve the antimicrobial effect against Bacillus cereus as well as inhibition of the growth on potato. (hocl.com)
It was determined that the antimicrobial activity of chlorine dioxide gas was higher than that of the aqueous, and spores were inactivated within one hour. (clordisys.com)

The S-layer produced by B. cereus AH187 is composed of two proteins, SL2 and EA1, which are incorporated at different growth stages. (bvsalud.org)

Several new enzymes have been discovered in B. cereus, such as AlkC and AlkD, both of which are involved in DNA repair. (wikipedia.org)

Furthermore, SAcEW US40 C treatment efficiently extended lag time of B. cereus by 0.210.5 hrs, reduced that of specific growth rate by 0.010.23 log CFU/h during storage at different temperatures from 5 to 35 C. Therefore, this combined hurdle technology is capable of improving microbial safety of potato during storage and distribution. (hocl.com)

To conclude, the combination of low pH and sodium benzoate provided the best method for spore inactivation, which could enhance food safety and extend food's shelf life. (hindawi.com)
In 2010, the Department of Food Science at Purdue University compared the effect of chlorine dioxide gas, aqueous chlorine dioxide, and aqueous sodium hypochlorite treatments on the inactivation of listeria monocytogenes containing biofilms. (clordisys.com)

Bacillus licheniformis " Encyclopedia , https://encyclopedia.pub/entry/46763 (accessed December 01, 2023). (encyclopedia.pub)

4) A select agent or regulated nucleic acids that can produce infectious forms of any select agent virus that has been subjected to a validated inactivation procedure that is confirmed through a viability testing protocol. (lawstack.com)

The specific name, cereus, meaning "waxy" in Latin, refers to the appearance of colonies grown on blood agar. (wikipedia.org)

Formaldehyde has been used for the inactivation of viruses in vaccine production for a number of years. (basicmedicalkey.com)

In the 2010s, examination of warning letters issued by the US Food and Drug Administration issued to pharmaceutical manufacturing facilities addressing facility microbial contamination revealed that the most common contaminant was B. cereus. (wikipedia.org)
This study indicated that EO water was highly effective in inactivation of B. subtilis and B. cereus spores in suspension or on carrier, and therefore, rendered it as a promising disinfectant to be applied in food industry. (hocl.com)
B. cereus food poisoning is the general description, although two recognized types of illness are caused by two distinct metabolites. (environsafety.com)
All people are believed to be susceptible to B. cereus food poisoning. (environsafety.com)

More rapid tests exploit antibodies associated to the surface of the spores (exosporium) for detection. (justia.com)
Sonication may also be used to modify the surface of spores so as to aid subsequent detection in an immunoassay, for instance immunoassays involve the binding of an analyte to a specific antibody contained on the surface of a sensor. (justia.com)
The emetic reference strain B. cereus AH187 is surrounded by a proteinaceous surface layer (S-layer) that contributes to its physico-chemical surface properties, and promotes its adhesion in response to starvation conditions. (bvsalud.org)
Microscopy analysis revealed the spatial organization of SL2 and EA1 at the surface of B. cereus to depend on their temporal-dynamics during growth. (bvsalud.org)

In this study, we used high-throughput proteomics to investigate how S-layer synthesis affects the allocation of cellular resources in the emetic B. cereus strain AH187. (bvsalud.org)
B. cereus Elc4, a strain isolated from a fatal case of inhalational anthrax-like disease, was also found to cause GI anthrax. (bvsalud.org)

These spores are highly resistant to various interventions and cooking processes. (hocl.com)

Bacillus subtilis produces 66 derived antimicrobials, and Bacillus brevis produces 23 peptide antibiotics [ 6 ] . (encyclopedia.pub)

The method involves irradiating a sample with laser light, conveniently ultraviolet radiation, to disrupt any spores present and collecting any disrupted material for analysis. (justia.com)
This invention relates to a method and apparatus for disrupting spores to aid subsequent analysis, especially to a method and apparatus for fast release of intrasporal DNA. (justia.com)
Another method of screening for spores is to completely disrupt the spore so as to release intrasporal DNA for subsequent analysis via polymerase chain reaction (PCR) assays. (justia.com)
It is therefore an object of the present invention to provide a method and apparatus for disrupting spores for subsequent detection which mitigates at least some of the aforementioned disadvantages. (justia.com)

We conclude that the synthesis of the S-layer is a critical adaptation for emetic B. cereus to successfully colonize specific niches. (bvsalud.org)

In our study, boiling water in a covered vessel for 3 to 5 min destroyed spores of the Bacillus spp. (cdc.gov)

Spores were harvested from the agar plates and washed four times by centrifugation with sterile distilled water, treated with 50% (vol/vol) ethanol while being shaken at 100 rpm for 2 h, then washed an additional four times by centrifugation with sterile distilled water. (cdc.gov)
Water samples were injected with the spore preparations, heated to boiling on a hot plate, and held at boiling temperature for various times. (cdc.gov)
Both types of EO water with ACC of 60 mg/L and 6 min treatment achieved a reduction of B. subtilis and B. cereus spores to nondetectable level. (hocl.com)
But, addition of 0.3 organics on carrier decreased the inactivation effect of EO water. (hocl.com)

Shotgun proteomics analysis indicated that SL2 is the major component of the B. cereus S-layer at the beginning of exponential growth, whereas EA1 becomes more abundant than SL2 during later stages of stationary growth. (bvsalud.org)

Anatomy5

Organisms13

Diseases6

Chemicals and Drugs21

Analytical, Diagnostic and Therapeutic Techniques and Equipment6

Phenomena and Processes18

Disciplines and Occupations2

Technology, Industry, Agriculture1

Information Science4

Health Care5

Anthracis9

Bacteria10

Subtilis spores1

Bacterial5

Staphylococcus2

Sensu lato1

Bacterium4

Strains7

Species5

Germinate1

Gram-positive2

Genus1

Vegetative2

Anaerobic1

Inactivate2

Biofilm2

Germination2

Thuringiensis2

Resistance2

Stainless steel1

Genome2

Cells2

Particles2

Predominant1

Reductions2

Outer1

Antimicrobial2

Proteins1

Enzymes1

Temperatures1

Sodium2

20231

Procedure1

Refers1

Viruses1

Food4

Surface4

Strain2

Resistant1

Produces1

Method4

Specific1

Study1

Water4

Analysis1