• Since its discovery in 2000, initial in vitro studies have found that the engagement of Siglec-8 with a monoclonal antibody or with selective polyvalent sialoglycan ligands induced the cell death of eosinophils and inhibited mast cell degranulation. (mdpi.com)
  • As CD314 ligands the MHC class-I chain-related proteins A and B (MICA, MICB) and UL16-binding proteins (ULBPs) have been identified. (exbio.cz)
  • Wu J, Groh V, Spies T: T cell antigen receptor engagement and specificity in the recognition of stress-inducible MHC class I-related chains by human epithelial gamma delta T cells. (exbio.cz)
  • BACKGROUND: In recent years, there has been great interest in developing molecular adjuvants based on antisense oligonucleotides (ASOs) targeting immunosuppressor pathways with inhibitory effects on regulatory T cells (Tregs) to improve immunogenicity and vaccine efficacy. (bvsalud.org)
  • Siglecs (sialic acid-binding immunoglobulin-like lectins) are single-pass cell surface receptors that have inhibitory activities on immune cells. (mdpi.com)
  • We have been interested in the role of pattern-recognition receptors including Toll-like receptors (TLRs)in innate immune recognition of these viruses as well as their signaling pathways. (dukecancerinstitute.org)
  • In addition, we are investigating the role of innate immune cells such as natural killer (NK) cells in innate and adaptive immune responses to these viruses. (dukecancerinstitute.org)
  • To elicit innate and adaptive immune responses, inflammatory cells must efficiently migrate through the complex, physiological environments in the body. (life-science-alliance.org)
  • Two additional cell types, both part of the innate immune system, are found within the sinusoids, in direct contact with the blood: a large number of macrophages, called Kupffer cells, and Pit cells, a type of natural killer (NK) cells. (helmberg.at)
  • Results: Tregs depletion enhanced the frequency of specific IFNγ+ T cells (Th1 lymphocytes) and cytokine production after either the first or second vaccine dose. (bvsalud.org)
  • A group of heterogeneous lymphoid tumors generally expressing one or more B-cell antigens or representing malignant transformations of B-lymphocytes. (lookformedical.com)
  • They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation. (lookformedical.com)
  • A form of non-Hodgkin lymphoma having a usually diffuse pattern with both small and medium lymphocytes and small cleaved cells. (lookformedical.com)
  • A classification of B-lymphocytes based on structurally or functionally different populations of cells. (lookformedical.com)
  • NK1.1 (Killer cell lectin-like receptor subfamily B, member 1, KLRB1, NKR-P1A, CD161, cluster of differentiation 161), refers to Natural Killer (NK) cells, lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. (thermofisher.com)
  • Separation of human CD314 positive CD56 positive NK cells (red-filled) from CD314 negative CD56 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD314 (1D11) APC antibody. (exbio.cz)
  • Description: The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in the NK1.1 mouse strains including C57BL and NZB. (thermofisher.com)
  • Anti-Siglec-8 antibody administration in vivo to humanized and transgenic mice selectively expressing Siglec-8 on mouse eosinophils and mast cells confirmed the in vitro findings, and identified additional anti-inflammatory effects. (mdpi.com)
  • Cells grown in vitro from neoplastic tissue. (lookformedical.com)
  • Applications Reported: PK136 has been reported for use in flow cytometric analysis, depletion of NK cells, and in vitro functional studies. (thermofisher.com)
  • Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. (rndsystems.com)
  • For detection of NK cells in these strains the monoclonal antibody DX5 (Cat. (thermofisher.com)
  • A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)
  • The mouse monoclonal antibody 1D11 recognizes an extracellular epitope of CD314 / NKG2D, a 42 kDa C-type lectin-like activating receptor expressed by NK cells, gamma/delta T cells, and CD8+ T cells. (exbio.cz)
  • The PK136 antibody has been reported to deplete NK cells, induce redirected lysis, block NK cell function, and induce NK cell proliferation. (rndsystems.com)
  • Killer cell lectin-like receptor subfamily A (KLRA, alternative nomenclature Ly49) is a gene cluster coding proteins from family Ly49, which are membrane receptors expressed mainly on the surface of NK cells and other cells of immune system in some mammals including rodents and cattle but not humans. (wikipedia.org)
  • Klra MGI Mouse Complex/Cluster/Region Detail - MGI:96876 - killer cell lectin-like receptor, subfamily A". www.informatics.jax.org. (wikipedia.org)
  • CD314, also known as NKG2D (natural killer receptor G2D) or KLRK1 (killer cell lectin-like receptor subfamily K, member 1), is a homodimeric C-type lectin-like activating receptor and costimulator with type II membrane orientation (C teminus extracellular). (exbio.cz)
  • The KLRB1 protein, NKR-P1A or CD161, is classified as a type II membrane protein because it has an external C terminus. (thermofisher.com)
  • CD314 homodimers are associated with DAP10, a membrane adaptor protein that signals similar to CD28 by recruitment of phosphatidylinositol 3-kinase. (exbio.cz)
  • It is likely that polyclonal B-cell lymphoproliferation in AIDS is a complex result of EBV infection, HIV antigenic stimulation, and T-cell-dependent HIV activation. (lookformedical.com)
  • The key role in the pathogenesis of sarcoidosis is played by proinflammatory cytokines and chemokines, molecules crucially involved in the activation of immune and inflammatory cells and their trafficking to the site of disease [4]. (thebiotechdictionary.com)
  • NK-1.1 has also been shown to play a role in NK cell activation, IFN- gamma production, and cytotoxic granule release. (rndsystems.com)
  • The majority of mantle-cell lymphomas are associated with a t(11;14) translocation resulting in overexpression of the CYCLIN D1 gene (GENES, BCL-1). (lookformedical.com)
  • NKR-P1A, the receptor encoded by the KLRB1 gene, recognizes Lectin Like Transcript-1 (LLT1) as a functional ligand. (thermofisher.com)
  • NK-1.1 surface antigen is encoded by the NKR-P1B/NKR-P1C gene, also known as CD161b/CD161c and Ly-55. (rndsystems.com)
  • It binds and inactivates the 140 kDa subunit of RNA polymerase II, blocking all gene expression leading to proteins. (helmberg.at)
  • Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5+PK136- and DX5-PK136+ cells. (thermofisher.com)
  • Engagement of CD314 amplifies antigen-specific T cell responses in CD314-positive T cell populations. (exbio.cz)
  • Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. (thermofisher.com)
  • In this model, only Foxp3(+) Tregs express eGFP and diphtheria toxin (DT) receptors, and transient Tregs depletion is achieved by DT administration. (bvsalud.org)
  • Supplementary MaterialsCorrelation network analysis reveals relationships between microRNAs, transcription factor and deregulated cytokine/chemokine-receptor network in pulmonary sarcoidosis 121378. (thebiotechdictionary.com)
  • Accumulating evidence has shown that the immunosuppressive CD4+CD25+Foxp3+ regulatory T cells (T Reg ) play a critical role in the suppression of anti-tumor immunity. (dukecancerinstitute.org)
  • Clinically, EBV-associated malignancies such as Hodgkin's lymphoma offer a unique model to explore antigen-defined immunotherapy approaches because EBV-derived tumor antigens are specific for tumor cells only. (dukecancerinstitute.org)
  • B-cell antigens are expressed on the immature cells that make up the tumor in virtually all cases of Burkitt lymphoma. (lookformedical.com)
  • If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely. (lookformedical.com)
  • This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth. (lookformedical.com)
  • Any of a group of malignant tumors of lymphoid tissue that differ from HODGKIN DISEASE, being more heterogeneous with respect to malignant cell lineage, clinical course, prognosis, and therapy. (lookformedical.com)
  • The only common feature among these tumors is the absence of giant REED-STERNBERG CELLS, a characteristic of Hodgkin's disease. (lookformedical.com)
  • The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain. (thermofisher.com)
  • recombinant enolase (rSsEno), a multifunctional protein with immunogenic properties, could be a promising target for vaccination against sporotrichosis in cats. (bvsalud.org)
  • Among these, Siglec-8 is a CD33-related family member selectively expressed on human mast cells and eosinophils, and at low levels on basophils. (mdpi.com)
  • Therefore, specific localization patterns of GEFs and GTPase-activating proteins, and a molecular network of their regulation underlie the spatiotemporal control of Rho GTPases activities in the cells. (life-science-alliance.org)
  • NK-1.1 and DX5 are commonly used as mouse NK cell markers. (rndsystems.com)
  • Structural and biochemical analyses reveal that DOCK8 DHR-1 domain consists of a C2 domain-like core with loops creating the upper surface pocket, where three basic residues are located for stereospecific recognition of phosphoinositides. (life-science-alliance.org)
  • Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10 6 cells in a suspension. (exbio.cz)
  • Eliciting long-lived memory T cell response is an ultimate goal of vaccination to provide long-term immunity against cancer. (dukecancerinstitute.org)
  • Lymphoid cells concerned with humoral immunity. (lookformedical.com)
  • These cells can participate in inflammatory responses by releasing mediators that attract or activate other cells, contributing to the pathogenesis of allergic and non-allergic diseases. (mdpi.com)
  • One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). (lookformedical.com)
  • All synthesized peptides exhibit B-cell epitopes on the molecular surface of SsEno and proved to be highly reactive with the serum of infected mice with S. brasiliensis and sera of cats with sporotrichosis. (bvsalud.org)
  • Intestinal epithelial cells ('dock workers') are good at moving stuff. (helmberg.at)
  • Introduction: Regulatory T cells (Tregs) have been shown to limit the protective immune response against pathogenic species of the fungus Sporothrix spp, the causal agent of sporotrichosis. (bvsalud.org)
  • Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. (lookformedical.com)
  • Chemistry of RNA and DNA base methylation The addition of the methyl-group to DNA and RNA bases (Fig.?2) is catalyzed by DNA- and RNA-methyltransferases that use DNMTs, which methylate canonical dC bases.26 In contrast, DNMT1 maintains the methylation status Actinomycin D biological activity during cell division. (thebiotechdictionary.com)
  • The understanding of mechanisms underlying memory T cell formation will provide important insights into the design of effective vaccines for treating cancer. (dukecancerinstitute.org)
  • For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. (rndsystems.com)
  • We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10 6 cells in 100 μL volume]. (elabscience.com)