ImmunoglobulinsCounter-immunoelectrophoresisASSAYELECTROPHORESISProteinImmunoglobulinUrineSpecificityMonoclonalCross-reactivityELISAAlkaline phosphatasePolyclonalAlbuminRabbit serumAssaysImmunofixationMonospecificReactionGoatAntibodiesDiagnosisHumanSterileNormalLesionsLevelsMultiplePatientsDoubleFree
Immunoglobulins7
- Serum immunoglobulins are commonly measured by radial immunodiffusion or immunoturbidimetric methods. (medscape.com)
- Electrophoresis and immunoelectrophoresis are not satisfactory techniques for the quantification of immunoglobulins. (medscape.com)
- The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, syrian hamster, horse, human, rabbit and sheep serum proteins, but it may cross-react with immunoglobulins from other species. (jacksonimmuno.com)
- By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. (abcam.com)
- The three immunoglobulins showed reactions of antigenic identity with the corresponding Ig classes of serum when examined with rabbit antisera against human IgG, IgA, and IgM. (nih.gov)
- This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis. (agrisera.com)
- Donkey IgG F(ab')2 fragment Peroxidase conjugated - Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. (rockland.com)
Counter-immunoelectrophoresis2
- The test was based on the detection of IgG antibodies to Pseudomonas mallei antigens bound to nitrocellulose coated on plastic strips (dipsticks), the reaction being amplified by an avidinbiotin system with biotinylated anti-horse IgG and horseradish peroxidase-avidin D. Sera from 810 normal, six naturally infected and 48 sensitized equines were tested by this assay and results were compared with complement fixation, indirect haemagglutination and counter-immunoelectrophoresis tests. (edu.in)
- Most workers reacted positively with Micropolyspora-faeni antigen, but is was not possible with either ELISA or counter immunoelectrophoresis to separate the ill from the workers who were not ill. (cdc.gov)
ASSAY5
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. (abcam.com)
- Enzyme linked immunosorbent assay (ELISA) tests for HIV and antibody titres against Epstein-Barr virus were also negative in both serum and CSF samples. (bmj.com)
- Assay by immunoelectrophoresis resulted in multiple precipitin arcs against anti-Donkey Serum. (rockland.com)
- Rarely, patients have no M-protein in blood and urine, although the currently used serum free light chain assay now demonstrates monoclonal light chains in many of these formerly so-called nonsecretory patients. (merckmanuals.com)
- In the case of serum protein immunoelectrophoresis, CMS proposes to pay for only one assay per day. (myelomablog.com)
ELECTROPHORESIS7
- Immunoelectrophoresis is a powerful analytical technique with high resolving power as it combines separation of antigens by electrophoresis with immunodiffusion against an antiserum. (wordinfo.info)
- Serum protein electrophoresis is mainly used for the diagnosis and monitoring of patients with monoclonal gammopathies. (athenslab.gr)
- Serum protein electrophoresis is a widely used method of measuring albumin and each of the types of globulins. (athenslab.gr)
- Serum protein electrophoresis is also used to investigate other abnormal laboratory results, such as when measuring total protein, albumin, and urine protein, low calcium levels, and low white and/or red blood cells. (athenslab.gr)
- When a monoclonal gammopathy is found by electrophoresis of serum proteins, multiple myeloma must be excluded from other causes. (athenslab.gr)
- This enhances the effect of serum protein electrophoresis. (athenslab.gr)
- Clinical data were gathered, serum protein electrophoresis was performed and immunoelectrophoresis was carried out for the detection of monoclonal component. (cu.edu.eg)
Protein5
- 1800) and a monoclonal protein band of IgG-κ type was detected by immunoelectrophoresis. (bmj.com)
- It does not react with IgG, IgM and IgG/Fab or any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. (fishersci.com)
- There are many phenotypes which lead to low serum levels of the protein. (southtees.nhs.uk)
- Diagnosis typically requires demonstration of M-protein (sometimes present in urine and not serum but rarely absent entirely) and/or light-chain proteinuria, and excessive plasma cells in the bone marrow. (merckmanuals.com)
- It is used to detect diseases such as multiple myeloma and other disorders in serum proteins, inflammatory conditions, autoimmune diseases, infections, and protein loss conditions. (athenslab.gr)
Immunoglobulin5
- A diagnosis of common variable immunodeficiency (CVID) is based on defective functional antibody formation, usually accompanied by decreased (not absent) serum immunoglobulin (Ig)G and IgA levels, generally (not invariably) decreased serum IgM, and exclusion of other known causes of antibody deficiency. (medscape.com)
- No antibody was detected against non-immunoglobulin serum proteins. (jacksonimmuno.com)
- Incubations of calcitonins-125I and sera from these six subjects resulted in the association of radioactivity with material whose behavior on chromatoelectrophoresis (6/6), sucrose density ultracentrifugation and immunoelectrophoresis (one subject) was identical with that of 7S immunoglobulin. (jci.org)
- Low levels of α1-antitrypsin may be detected inadvertently during serum immunoelectrophoresis which is most commonly being used to investigate immunoglobulin levels. (southtees.nhs.uk)
- Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis. (agrisera.com)
Urine2
- Serum or urine bromide and iodide levels should be measured. (medscape.com)
- It is a separation technique, easy, simple and highly sensitive test and used on body fluids such as blood serum ,urine and CSF. (slideshare.net)
Specificity1
- The anti-human lactoferrin was solid phase absorbed against human serum to ensure specificity. (leebio.com)
Monoclonal1
Cross-reactivity1
ELISA1
- Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. (jacksonimmuno.com)
Alkaline phosphatase2
Polyclonal3
- Our Polyclonal Antibody anti-human lactoferrin forms a single precipitation arc against human lactoferrin by immunoelectrophoresis. (leebio.com)
- Bovine IgG is a preparation of purified polyclonal bovine IgG extracted frrom bovine serum, By immunoelectrophoresis, this product react with antiserum specific for bovine IgG and subclasses IgG1 and IgG2. (immunoportal.com)
- Preservative: 0.09% Sodium Azide.The serum is polyclonal, delipidated and monospecific by immunoelectrophoresis. (enzytec.com)
Albumin3
- An indirect indication of loss may be obtained by measuring serum albumin or alpha-1-antitrypsin, which is usually lost concomitantly through GI or renal tracts. (medscape.com)
- Total serum proteins consist of albumin and globulins. (athenslab.gr)
- Serum Albumin. (bvsalud.org)
Assays2
- Donkey Serum is used as a component of bioassays, immunoassays or enzyme assays. (rockland.com)
- Donkey serum is suitable for use as a control or diluent in assays and most other immunological methods. (rockland.com)
Immunofixation1
- This can be done by a method called immunofixation or immunoelectrophoresis. (athenslab.gr)
Monospecific1
- The direct Coombs test (direct antiglobulin test [DAT]) should be performed with samples at 35-37°C, using polyspecific and monospecific Coombs sera, including monospecific anti-C3 and IgG antisera. (medscape.com)
Reaction1
- There is no reaction with normal human serum. (leebio.com)
Goat7
- Goat Serum is used as a supplement to cell culture media. (generon.ie)
- Goat Serum provides a broad spectrum of macromolecules, carrier proteins for lipoid substances and trace elements, attachment and spreading factors, low molecular weight nutrients, and hormones and growth factors that promote cell growth and health. (generon.ie)
- Sterile Goat Serum - Heat Inactivated was prepared by removal of fibrinogen through clot formation. (generon.ie)
- Goat Serum was separated from cellular components and purified through centrifugation and filtration techniques. (generon.ie)
- Goat Serum was sterilized using a 0.22 micron filter and found to be negative for bacterial growth after incubation on trypticase soy agar for 24 hours, 48 hours, and 72 hours. (generon.ie)
- Goat Purified Anti-serum to Normal Human Serum. (enzytec.com)
- This Goat Anti-Mouse IgG, H & L Chain β-Galactosidase Conjugate is validated for use in Enzyme Immunoassay, Immunoelectrophoresis for the detection of Mouse IgG, H & L Chain. (emdmillipore.com)
Antibodies4
- The increase in this serum globulin coincides closely with the appearance of antibodies to malaria as detected by fluorescent antibody studies. (ajtmh.org)
- Competitive displacement of calcitonins-125I from the sera of one patient treated with both porcine and salmon calcitonin indicated separate populations of antibodies to these hormones. (jci.org)
- In immunoelectrophoresis, the specific proteins of interest can be determined after first being fixed in the gel with antibodies, and by removing all other proteins by washing, prior to staining. (athenslab.gr)
- An evaluation was conducted to describe the exposure to bacteria, fungi, and endotoxins as well as in-vitro and in-vivo immunologic studies related to the presence of specific antibodies and serum complement activation in farm workers who shoveled approximately 800 bushels of oats from a poorly ventilated storage bin. (cdc.gov)
Diagnosis1
- Immunoelectrophoresis aids in the diagnosis and evaluation of the therapeutic response in many disease conditions affecting the immune system. (wordinfo.info)
Human3
- Purified IgA isolated from human serum. (fishersci.com)
- D-Alanyl LTA in the focus was discovered by immunoelectrophoresis, utilizing a standard human serum discovered [16] previously. (euromed2016.com)
- Purified IgG prepared from pooled human serum. (exalpha.com)
Sterile1
- Donkey serum is available as a sterile preparation for use as a cell culture supplement. (rockland.com)
Normal3
- Some rarer phenotypes give normal serum levels of an inactive α1-antitrypsin. (southtees.nhs.uk)
- Donkey serum was prepared from normal serum by delipidation and defibrination. (rockland.com)
- This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. (rockland.com)
Lesions1
Levels1
- Serum Ig levels are consistently depressed but are generally higher in patients with common variable immunodeficiency than in patients with X-linked agammaglobulinemia. (medscape.com)
Multiple1
- After multiple immunizations the rabbits were bled and serum collected. (leebio.com)
Patients2
- Specific, reversible in vitro binding of salmon calcitonins-125I was observed in sera obtained from these patients 5 to 12 months after initiation of salmon calcitonin therapy. (jci.org)
- The disappearance of total radioactivity from the circulation after intravenous administration of salmon calcitonin-125I was retarded and the amount of serum radioactivity precipitable in 50% (NH4)2SO4 greater in 3/3 resistant patients compared to control subjects. (jci.org)
Double1
- Thirty-six fluids from non-keratinizing jaw cysts have been examined together with autologous sera by immunoelectrophoresis and double diffusion in agar or agrose gels. (nih.gov)
Free1
- D-Alanine-free LTA will not react with this serum IgG [17,18,21]. (euromed2016.com)