• The test was based on the detection of IgG antibodies to Pseudomonas mallei antigens bound to nitrocellulose coated on plastic strips (dipsticks), the reaction being amplified by an avidinbiotin system with biotinylated anti-horse IgG and horseradish peroxidase-avidin D. Sera from 810 normal, six naturally infected and 48 sensitized equines were tested by this assay and results were compared with complement fixation, indirect haemagglutination and counter-immunoelectrophoresis tests. (edu.in)
  • Most workers reacted positively with Micropolyspora-faeni antigen, but is was not possible with either ELISA or counter immunoelectrophoresis to separate the ill from the workers who were not ill. (cdc.gov)
  • A diagnosis of common variable immunodeficiency (CVID) is based on defective functional antibody formation, usually accompanied by decreased (not absent) serum immunoglobulin (Ig)G and IgA levels, generally (not invariably) decreased serum IgM, and exclusion of other known causes of antibody deficiency. (medscape.com)
  • No antibody was detected against non-immunoglobulin serum proteins. (jacksonimmuno.com)
  • Incubations of calcitonins-125I and sera from these six subjects resulted in the association of radioactivity with material whose behavior on chromatoelectrophoresis (6/6), sucrose density ultracentrifugation and immunoelectrophoresis (one subject) was identical with that of 7S immunoglobulin. (jci.org)
  • Low levels of α1-antitrypsin may be detected inadvertently during serum immunoelectrophoresis which is most commonly being used to investigate immunoglobulin levels. (southtees.nhs.uk)
  • Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis. (agrisera.com)
  • Serum or urine bromide and iodide levels should be measured. (medscape.com)
  • It is a separation technique, easy, simple and highly sensitive test and used on body fluids such as blood serum ,urine and CSF. (slideshare.net)
  • The anti-human lactoferrin was solid phase absorbed against human serum to ensure specificity. (leebio.com)
  • Immunoelectrophoresis of CSF showed a monoclonal IgG-κ band similar to the results of serum assays whereas biochemical and cytopathological CSF examinations were normal. (bmj.com)
  • To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species. (abcam.com)
  • All of the subjects experienced a striking decrease in serum alkaline phosphatase during the first 4 months of treatment. (jci.org)
  • These rebounds in alkaline phosphatase levels correlated with the appearance of calcitonin-binding substances and neutralizing material in serum. (jci.org)
  • An indirect indication of loss may be obtained by measuring serum albumin or alpha-1-antitrypsin, which is usually lost concomitantly through GI or renal tracts. (medscape.com)
  • Total serum proteins consist of albumin and globulins. (athenslab.gr)
  • Serum Albumin. (bvsalud.org)
  • Two cyst fluids also precipitated a component in rabbit serum. (nih.gov)
  • Donkey Serum is used as a component of bioassays, immunoassays or enzyme assays. (rockland.com)
  • Donkey serum is suitable for use as a control or diluent in assays and most other immunological methods. (rockland.com)
  • The direct Coombs test (direct antiglobulin test [DAT]) should be performed with samples at 35-37°C, using polyspecific and monospecific Coombs sera, including monospecific anti-C3 and IgG antisera. (medscape.com)
  • Goat Serum is used as a supplement to cell culture media. (generon.ie)
  • Goat Serum provides a broad spectrum of macromolecules, carrier proteins for lipoid substances and trace elements, attachment and spreading factors, low molecular weight nutrients, and hormones and growth factors that promote cell growth and health. (generon.ie)
  • Sterile Goat Serum - Heat Inactivated was prepared by removal of fibrinogen through clot formation. (generon.ie)
  • Goat Serum was separated from cellular components and purified through centrifugation and filtration techniques. (generon.ie)
  • Goat Serum was sterilized using a 0.22 micron filter and found to be negative for bacterial growth after incubation on trypticase soy agar for 24 hours, 48 hours, and 72 hours. (generon.ie)
  • Goat Purified Anti-serum to Normal Human Serum. (enzytec.com)
  • This Goat Anti-Mouse IgG, H & L Chain β-Galactosidase Conjugate is validated for use in Enzyme Immunoassay, Immunoelectrophoresis for the detection of Mouse IgG, H & L Chain. (emdmillipore.com)
  • The increase in this serum globulin coincides closely with the appearance of antibodies to malaria as detected by fluorescent antibody studies. (ajtmh.org)
  • Competitive displacement of calcitonins-125I from the sera of one patient treated with both porcine and salmon calcitonin indicated separate populations of antibodies to these hormones. (jci.org)
  • In immunoelectrophoresis, the specific proteins of interest can be determined after first being fixed in the gel with antibodies, and by removing all other proteins by washing, prior to staining. (athenslab.gr)
  • An evaluation was conducted to describe the exposure to bacteria, fungi, and endotoxins as well as in-vitro and in-vivo immunologic studies related to the presence of specific antibodies and serum complement activation in farm workers who shoveled approximately 800 bushels of oats from a poorly ventilated storage bin. (cdc.gov)
  • Immunoelectrophoresis aids in the diagnosis and evaluation of the therapeutic response in many disease conditions affecting the immune system. (wordinfo.info)
  • Donkey serum is available as a sterile preparation for use as a cell culture supplement. (rockland.com)
  • Some rarer phenotypes give normal serum levels of an inactive α1-antitrypsin. (southtees.nhs.uk)
  • Donkey serum was prepared from normal serum by delipidation and defibrination. (rockland.com)
  • This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. (rockland.com)
  • By the third month of follow up, serum and CSF paraprotein disappeared and lytic skull lesions regressed. (bmj.com)
  • Serum Ig levels are consistently depressed but are generally higher in patients with common variable immunodeficiency than in patients with X-linked agammaglobulinemia. (medscape.com)
  • Specific, reversible in vitro binding of salmon calcitonins-125I was observed in sera obtained from these patients 5 to 12 months after initiation of salmon calcitonin therapy. (jci.org)
  • The disappearance of total radioactivity from the circulation after intravenous administration of salmon calcitonin-125I was retarded and the amount of serum radioactivity precipitable in 50% (NH4)2SO4 greater in 3/3 resistant patients compared to control subjects. (jci.org)
  • Thirty-six fluids from non-keratinizing jaw cysts have been examined together with autologous sera by immunoelectrophoresis and double diffusion in agar or agrose gels. (nih.gov)