ProteinsMicrobiologyEscherichiaGenomesAbstractGenomeRegulationFungal2017ResistanceStrainsPlos GeneticsChromosomalVirulence factorsPlasmidsEncodeMicrobiomeMutationGeneticProteinPopulation geneticsBacteriaFrontiersMolecularCas9Range of bacterialStrainSpecies2019Expression2020CRISPRRRNA2001Horizontal gene transfer eClonesInfectionCompartmentsViralRestoresGenetically
Proteins8
- Bacterial proteases are important enzymes used in several technical applications where controlled cleavage of proteins is needed. (lu.se)
- The eukaryotic model organism Saccharomyces cerevisiae is potentially a good expression host as it tolerates several stress conditions and is known to better express insoluble proteins compared to bacterial systems. (lu.se)
- 19 In 1987, Plant Genetic Systems (Ghent, Belgium), founded by Marc Van Montagu and Jeff Schell, was the first company to develop genetically engineered (tobacco) plants with insect tolerance by expressing genes encoding for insecticidal proteins from Bacillus thuringiensis (Bt). (truthwiki.org)
- The Popeye domain containing (POPDC) genes encode sarcolemma-localized cAMP effector proteins. (imperial.ac.uk)
- Three of the six up-regulated genes encode potential zinc transporter proteins (ZnuA1, ZnuB1, ZnuC1) suggesting involvement of s479 in regulation of zinc transport. (uni-stuttgart.de)
- Interestingly, s479 is not only encoded next to CRISPR-cas genes but the mature s479 contains a crRNA-like 5´ handle and experiments with Cas protein deletion strains indicate maturation by Cas6 and interaction with Cas proteins. (uni-stuttgart.de)
- 1. Moody R G,Williamson M P. Structure and function of a bacterial Fasciclin Ⅰ domain protein elucidates function of related cell adhesion proteins such as TGFBIp and periostin[J]. FEBS Open Biology,2013,3(1):71-77. (nefu.edu.cn)
- Poplar genes encoding fasciclin-like arabinogalactan proteins are highly expressed in tension wood[J]. New Phytologist,2004,164(1):107-121. (nefu.edu.cn)
Microbiology1
- Un 40% de esos trabajos han sido publicados en revistas del primer cuartil entre las que destacan New England Journal of Medicine, Lancet, Lancet Infectious Diseases, Clinical Infectious Diseases, Clinical Microbiology Reviews, Antimicrobial Agents and Chemotherapy, Journal Clinical Microbiology, Journal Antimicrobial Chemotherapy, Mayo Clinic Proceedings y Malaria Journal. (unav.edu)
Escherichia1
- Background: Infection in the oviduct (salpingitis) is the most common bacterial infection in egg laying hens and is mainly caused by Escherichia coli. (ku.dk)
Genomes6
- While thousands of bacterial genomes are available to date, there is no software that facilitates comparisons of individual gene loci for a large number of genomes. (chalmers.se)
- The phage genomes displayed modular organisation and conserved viral genes were identified which enabled further classification and determination of closest phage relatives. (qld.gov.au)
- Co-examination of bacterial host genomes led to the identification of several genes responsible for modulating phage:host interactions, including CRISPR/Cas elements and restriction-modification phage defence systems. (qld.gov.au)
- Twenty of the 62 genomes harboured at least one antimicrobial resistance gene with resistance against sulfonamides being the most common. (ku.dk)
- The increased serum survival and iron chelating genes iss and iroN were highly prevalent in genomes from both acute and chronic salpingitis. (ku.dk)
- Professor Louis S. Tisa, University of New Hamsphire, USA, presented his recent accomplishments for genes, genomes and genetics of Frankia and non-Frankia bacteria involved in the actinorhizal symbiosis. (comstech.org)
Abstract2
- abstract = "Bacterial flagellar rod structure is built across the peptidoglycan (PG) layer. (elsevierpure.com)
- Abstract: Bacterial whole genome sequence (WGS) methods are rapidly overtaking classical sequence analysis. (unican.es)
Genome9
- Using genome-scale metabolic network modelling combined with experimental tools they studied key issues in evolution, such as mutational robustness, horizontal gene transfer, genome reduction, epistasis, promiscuous enzyme reactions, and complex adaptations. (wikipedia.org)
- The most frequent target of resistance mutations was the plasmid-encoded SpCas9 that was inactivated by bacterial genome rearrangements involving translocation of mobile genetic elements such as insertion elements. (dtu.dk)
- To facilitate M. grisea genome analysis, physical mapping, and positional cloning, we have constructed a bacterial artificial chromosome (BAC) library from the rice infecting strain 70-15. (johnshopkins.edu)
- Microbiological, quantitative gene expression, mRNA half-life and genome data for 11 strains of S. aureus combined with bioinformatic analyses were used to identify correlates of increased MDR EP gene expression. (manchester.ac.uk)
- Genome-Wide Association Study Reveals Novel Candidate Genes Associated with Productivity and Disease Resistance to Moniliophthora spp. (apsnet.org)
- While bacterial genome sequencing projects are revealing the presence of phages which can integrate their DNA into the genome of their host to form stable, lysogenic associations, little is known of the genetics of phages which utilise lytic replication. (qld.gov.au)
- In vivo experiments demonstrated efficient genome editing of the Hao1 and Ldha genes, primarily resulting in small deletions. (zoores.ac.cn)
- We demonstrated the AAV-AsCpf1 system can target multiple genes and rescue the pathogenic phenotype in PH1, serving as a proof-of-concept for the development of multiplex genome editing-based gene therapy. (zoores.ac.cn)
- Each instrument can generate data required for a draft bacterial genome sequence in days, making them attractive for identifying and characterizing pathogens in the clinical setting. (nature.com)
Regulation2
- Role and regulation of ACC deaminase gene in Sinorhizobium meliloti: is it a symbiotic, rhizospheric or endophytic gene? (unifi.it)
- Transcriptome studies of wild type Haloferax cells and the deletion mutant revealed up-regulation of six genes in the deletion strain, showing that the sRNA has a clearly defined function. (uni-stuttgart.de)
Fungal3
- Fungal Genetics and Biology , 21 (3), 337-347. (johnshopkins.edu)
- No fungal, bacterial, or mycobacterial pathogens were isolated from the pericardial effusion. (spandidos-publications.com)
- There is not yet evidence that the nature and strength of these interactions differs between fungal and bacterial communities, or among different compartments of the plant. (nyu.edu)
20171
- ViCAR: An Adaptive and Landmark-Free Registration of Time Lapse Image Data from Microfluidics Experiments", Frontiers in Genetics , vol. 8, 2017, : 69. (uni-bielefeld.de)
Resistance5
- 7 It is now known that there are several natural mechanisms for flow of genes, or (horizontal gene transfer), and that these occur in nature on a large scale - for example, it is a major mechanism for antibiotic resistance in pathogenic bacteria, and it occurs between plant species. (truthwiki.org)
- 14 Since then important traits have been introduced into wheat, including dwarfing genes and rust resistance. (truthwiki.org)
- Regulatory mechanisms for chromosomal genes encoding multidrug resistance (MDR) efflux pumps (EPs) in Staphylococcus aureus are poorly defined. (manchester.ac.uk)
- We have recently described the mpr gene of Mycobacterium smegmatis whose product confers resistance to mycobacteriophages L5 and D29 when overproduced. (pitt.edu)
- ST131 is a globally spread clonal group of extraintestinal pathogenic E. coli (ExPEC), comprising different sublineages with ability to acquire and spread antibiotic resistance and virulence genes via plasmids. (unican.es)
Strains5
- Comparing genomic loci of a given bacterial gene across strains and species can provide insights into their evolution, including information on e.g. acquired mobility, the degree of conservation between different taxa or indications of horizontal gene transfer events. (chalmers.se)
- In the US, strains of African and European origin have remained genetically distinct, while in Colombia and Nicaragua, bottlenecks and rampant genetic exchange amongst isolates have led to the formation of national gene pools. (bath.ac.uk)
- The presence of qacA/B and/or increased expression of one to two MDR EP genes were identified in eight strains. (manchester.ac.uk)
- Slime was occasionally produced by S. caprae strains but all of them carried nucleotide sequences hybridizing at low stringency with the following genes: atlE encoding a S. epidermidis autolysin binding vitronectin and responsible for the primary adhesion to polystyrene, ica operon involved in the biosynthesis of a S. epidermidis extracellular polysaccharide, and the part of clfA encoding the serine-aspartate repeated region of a S. aureus cell-wall fibrinogen-binding protein. (unige.ch)
- Significant differences were detected between the different strains in terms of bacterial size, intermembrane distance and its positional fluctuations. (iucr.org)
Plos Genetics1
- Plos Genetics , 13 (2), 1-21. (bath.ac.uk)
Chromosomal2
- Bacterial mutagenicity and mammalian cell chromosomal and DNA damage in vitro assays were performed on a diesel exhaust particulate material (DPM) standard in two preparations: as an organic solvent extract, and as an aqueous dispersion in a simulated pulmonary surfactant. (cdc.gov)
- In this study, 40 patients with non-specific X linked mental retardation were analysed with full coverage, X chromosomal, bacterial artificial chromosome arrays. (ncl.ac.uk)
Virulence factors1
- Therefore, the presence of S. mutans in individuals with low caries experiences could be explained by differences in bacterial virulence factors or endogenous factors within the host populations. (bvsalud.org)
Plasmids1
Encode1
- The bacterial activator protein NorR binds to enhancer-like elements, upstream of the promoter site, and activates 54-dependent transcription of genes that encode nitric oxide detoxifying enzymes (NorVW), in response to NO stress. (strath.ac.uk)
Microbiome5
- However, association between human genetics and microbiome are less studied. (ut.ee)
- An international consortium MiBioGen with data from more than 18,000 individuals was formed with the aim to study the effect of host genetics on gut microbiome composition. (ut.ee)
- We identified 31 associations between genetic loci and microbiome as well as confirmed an association between a gene responsible for lactase production (LCT) and Bifidobacterium - an association seen in previous publications as well as replicated in new research studies. (ut.ee)
- Our work with PCOS and gut microbiome revealed that the effects of microbiome on PCOS work through metabolic processes and prediabetic women with PCOS diagnosis have lower bacterial diversity compared to their healthy counterparts. (ut.ee)
- In conclusion, with our work we contribute to the microbiome research field by bringing new knowledge into the interplay between microbiome and genetics as well as female health that in the future would provide an impetus for further in-depth research to fully understand the role of microbiome in human health and disease. (ut.ee)
Mutation2
- In 2007, Pal and colleagues demonstrated that antagonistic co-evolution with parasites has a large impact on the evolution of bacterial mutation rate. (wikipedia.org)
- Cas9/gRNA-mediated Targeted PtrFLA 31/34 Gene Mutation in Populus [J]. Bulletin of Botanical Research, 2018, 38(5): 741-747. (nefu.edu.cn)
Genetic6
- Population genetic theory and tools and related strategies have been used to investigate bacterial pathogens that have contributed to recent episodes of temporal variation in disease frequency and severity. (cdc.gov)
- Because substantial interclonal variance exists in relative virulence, molecular population genetic studies have led to the concept that the unit of bacterial pathogenicity is the clone or cell line. (cdc.gov)
- Genes and Genetic Systems , 81 (6), 381-389. (elsevierpure.com)
- Genetic analyses of FBLIM1 did not provide evidence that this gene is relevant in our patient group. (biomedcentral.com)
- Current hypotheses in SAPHO suggest a genetic predisposition in combination with a bacterial infection, resulting in reactive osteitis [ 3 , 13 ]. (biomedcentral.com)
- Based on the genetic similarity of the isolates and pattern of amino acid variations identified by partial sequencing of the gtfB gene, base-pair changes were identified and correlated with different virulence patterns among the isolates. (bvsalud.org)
Protein5
- The hypothesis offers a synthesis on seemingly unrelated problems such as the evolution of dominance, gene duplicability and co-evolution of protein complexsubunits. (wikipedia.org)
- The protocol provided may be adopted for other bacterial proteases through minor modifications of the fused protein. (lu.se)
- The sRNA gene is encoded between a CRISPR RNA locus and the Cas protein gene cluster, the s479 deletion strain is viable and was characterized in detail. (uni-stuttgart.de)
- The compact Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) from the Prevotella and Francisella 1 (Cpf1) protein simplifies multiplex gene editing and allows for all-in-one adeno-associated virus (AAV) delivery. (zoores.ac.cn)
- Flaws in the one gene-one protein model, the foundation of transgenics, are reviewed in the context of the recent and ongoing restructuring of the science of genetics. (isa-agrifood.com)
Population genetics1
Bacteria2
- Mobile group II introns ("targetrons") expressed through an m-toluic acid-inducible promoter from a broad-host-range vector containing an RK2 minireplicon is used for efficient gene targeting in a variety of gram-negative bacteria. (readabstracts.com)
- This broad-host-range system for targetron expression might facilitate gene targeting in a wide range of bacteria. (readabstracts.com)
Frontiers1
- Frontiers in Genetics 8: 69. (uni-bielefeld.de)
Molecular2
- Continued new insights into host-parasite interactions at the molecular level will be achieved by combining clonal analysis of bacterial pathogens with large-scale comparative sequencing of virulence genes. (cdc.gov)
- En mayo de 2007 obtuve el Certificate of Training in Molecular Biological Techniques en el Department of Molecular Biology y desde Junio de 2008 a Junio de 2009 completé el Certificate in Clinical Research en el Center for Translational Science Activities en Mayo Clinic College of Medicine, Rochester Minnesota. (unav.edu)
Cas91
- In order to study their functions in wood formation, we knocked out PtrFLA 31/34 double genes via Cas9/gRNA, and obtained three fla 31/34 double mutants. (nefu.edu.cn)
Range of bacterial2
- The method is unique as it allows systematic comparison of mutational effects and epistasis across a wide range of bacterial species. (wikipedia.org)
- A range of bacterial ( Bartonella spp, Mycoplasma spp. (bvsalud.org)
Strain2
- Isayenkov, SV & Maathuis, FJM 2015, ' The expression of rice vacuolar TPK channels genes restores potassium uptake in E. coli mutant strain LB2003 ', Cytology and Genetics , vol. 49, no. 1, pp. 1-5. (york.ac.uk)
- Upregulation of these genes in the deletion strain was confirmed by northern blot and proteome analyses. (uni-stuttgart.de)
Species2
- It is well known that while core cellular processes are generally conserved during evolution, the underlying genes differ somewhat between related species. (wikipedia.org)
- Variation of host control within species is governed by many genes of small effect and is sensitive to biotic and abiotic environments. (nyu.edu)
20191
- Obtuve en 2019 la Acreditación en Investigación Avanzada por la AQU. (unav.edu)
Expression6
- To reduce potentially toxic effect on the host cell, the growth and production phases were separated by using the inducible promoter GAL1p to control recombinant gene expression. (lu.se)
- Our results show that expression of these channels in the bacterial mutant restored the LB2003 growth on low K + media. (york.ac.uk)
- Microbiological and gene expression data correlated in four instances, existing knowledge of the substrate specificity of NorC resulted in correlation in two others, and a transcriptional/translational disconnect is possible for the remaining two. (manchester.ac.uk)
- The correlations proposed provide a guide for further experimentation leading to a better understanding of MDR EP gene expression in this important pathogen. (manchester.ac.uk)
- Identification of novel genes in Arabidopsis involved in secondary cell wall formation using expression profiling and reverse genetics[J]. The Plant Cell,2005,17(8):2281-2295. (nefu.edu.cn)
- The last session on Plant-Microbe interactions was opened by Professor Luis G Wall, University of Quilmes, Argentina, who presented the major plant cellular remodelling associated with intercellular Frankia root colonization of the actinorhizal host Discaria trinervis revealed through site-specific subtilase gene expression. (comstech.org)
20201
- Baisakh, N and Sachdeva, S and Bharadwaj, C and Singh, R K and Jain, P K and Patil, B S and Roorkiwal, M and Varshney, R K (2020) Characterization of ASR gene and its role in drought tolerance in chickpea (Cicer arietinum L.). PLOS ONE (TSI), 15 (7). (icrisat.org)
CRISPR1
- The most efficient and specific CRISPR RNA (crRNA) pairs targeting the rat Hao1 and Ldha genes were initially screened ex vivo . (zoores.ac.cn)
RRNA3
- In addition, IGS sequencing (e.g., sequencing of intergenic regions between the 16S and 23S rRNA genes) has been used to taxonomy studies, while partial sequencing of the gene gtfB , which encodes the enzyme glucosyltransferase B, has been used to investigate enzymatic activity and virulence 11-12 . (bvsalud.org)
- Figure 1: OTU number as a function of percentage sequence difference for 90 pyrosequenced 16S rRNA gene clones of known sequence. (nature.com)
- Figure 2: Proportion of sequences assigned to the correct OTU as a function of percentage sequence difference for pyrosequenced 16S rRNA gene clones of known sequence. (nature.com)
20013
- In 2001, Csaba Pal and colleagues demonstrated that highly expressed genes in yeast evolve slowly. (wikipedia.org)
- Realicé la Especialidad de Microbiología y Parasitología vía MIR en el Servicio de Microbiología de la Clínica Universitaria de Navarra (1997-2001). (unav.edu)
- En mayo de 2016 obtuve la exención del requisito de pertenecer al Cuerpo de Profesores Titulares de Universidad para participar en las pruebas de acreditación nacional para el Cuerpo de Catedráticos de Universidad por parte del Consejo de Universidades (artículo 60 de la Ley Orgánica 6/2001 de 21 de diciembre de Universidades). (unav.edu)
Horizontal gene transfer e1
- Strategy for in situ detection of natural transformation-based horizontal gene transfer events. (readabstracts.com)
Clones3
- A common theme demonstrated by these analyses is that distinct bacterial clones are responsible for disease outbreaks and increases in infection frequency. (cdc.gov)
- Many of these clones are characterized by unique combinations of virulence genes or alleles of virulence genes. (cdc.gov)
- The library was used to identify BAC clones containing a adenylate cyclase gene (mac1). (johnshopkins.edu)
Infection1
- As bacterial and viral infections can both cause the same kinds of symptoms, it can be difficult to distinguish which is the cause of a specific infection. (wikipedia.org)
Compartments1
- Specifically, this approach provides detailed insight into structural features of the cellular envelope, including the distance of the inner and outer membranes, as well as the scattering length densities of all bacterial compartments. (iucr.org)
Viral1
- [12] Distinguishing the two is important, since viral infections cannot be cured by antibiotics whereas bacterial infections can. (wikipedia.org)
Restores1
- They demonstrated that gene loss initiates adaptive genomic changes that rapidly restores fitness, but this process has substantial pleiotropic effects on cellular physiology and evolvability upon environmental change. (wikipedia.org)
Genetically1
- While the precise causes of Crohn's disease are unknown, it is believed to be caused by a combination of environmental, immune , and bacterial factors in genetically susceptible individuals [8] . (martinpollins.com)