• In addition, pulmonary expression of interferon alpha and beta and Irf7 specific pattern recognition receptors (PRR) and signaling molecules (Ddx58, Ifih1, Dhx58, ISGF3) were induced, an effect that showed specificity when compared to other inflammatory exposures. (cdc.gov)
  • ISGF3 is assembled in the CYTOPLASM and translocated to the CELL NUCLEUS in response to INTERFERON signaling. (nih.gov)
  • It consists of ISGF3-GAMMA and ISGF3-ALPHA, and it regulates expression of many interferon-responsive GENES . (nih.gov)
  • This ISGF3 complex translocates to the nucleus, binds IFN-stimulated response elements (ISREs) in DNA, and initiates the activation of several hundred interferon stimulated genes (ISGs) [ 8 , 9 ]. (scientificarchives.com)
  • The derived sequence of H-ICSBP reveals restricted homology within the first 120 amino acids to three other interferon regulatory factors, IRF-1, IRF-2, and ISGF3γ. (johnshopkins.edu)
  • Interferon beta appears to induce amoeboid morphology by maintaining high levels of the ISGF3 complex, which is composed of the heterodimer of STAT 1 and STAT 2 proteins and the IRF9 protein. (nusl.cz)
  • Activated STAT1 and STAT2 together with IRF-9 (p48) form a trimeric transcription factor complex (ISGF3). (medchemexpress.com)
  • This multiprotein transcription factor is termed ISGF3. (lu.se)
  • Activated STAT1 and STAT2 monomers dimerize and interact with IRF9 (IFN-regulatory factor 9) in the cytoplasm, which is known as the ISGF3 (IFN-stimulated gene (ISG) factor 3) complex [ 1 , 7 ]. (scientificarchives.com)
  • Nsp1β inhibits phosphorylation of STAT1 and nuclear translocation of ISGF3 complex (composed of STAT1, STAT2 and IRF9). (expasy.org)
  • For the second wave of IFN signaling, PRRSV Nsp1β blocks the phosphorylation of STATs and inhibits the nuclear translocation of ISGF3 complex (composed of STAT1, STAT2 and IRF9). (expasy.org)
  • Inhibiting the expression of IRF9 (the DNA-binding component of U-ISGF3) reduced the migration of mesenchymal/CSCs. (stanfordhealthcare.org)
  • Upon IFN treatment, STAT1 and STAT2 become tyrosine phosphorylated, assemble as heterodimers that bind IRF9 to form the ISGF3 complex. (rockland.com)
  • In response to IFN alpha/beta, three subunits (STAT1-alpha, STAT1-beta, STAT2) of ISGF3, become phosphorylated on tyrosine, migrate into the nucleus, and assemble into a complex together with ISGF3 gamma (p48), a DNA-binding protein that specifically binds to the IFN-stimulated response element. (lu.se)
  • The formed ISGF3 complexes then translocate to the nucleus and promotes the production of IFN-stimulated genes (ISGs) such as IRF7, MX1, and OAS1 [2] . (medchemexpress.com)
  • The CSC-like properties were correlated with high levels of unphosphorylated IFN-stimulated gene factor 3 (U-ISGF3), which was previously linked to resistance to DNA damage. (stanfordhealthcare.org)
  • Ourthiague DR, Birnbaum H, Ortenlöf N, Vargas JD, Wollman R, Hoffmann A. Limited specificity of IRF3 and ISGF3 in the transcriptional innate-immune response to double-stranded RNA. . (ucla.edu)
  • Consta de ISGF-3 GAMMA e ISGF3-ALFA, y regula la expresión de muchos GENES sensibles al interferón. (bvsalud.org)
  • Taken together, our findings indicate that repressed IFN signaling in TNBCs with CSC-like properties is due to high levels of U-ISGF3 and that treatment with IFN-ß reduces CSC properties, suggesting a therapeutic strategy to treat drug-resistant, highly aggressive TNBC tumors. (stanfordhealthcare.org)
  • Instead, a specific subset of ISGs that includes Parp9 remains activated by the unphosphorylated form of ISGF3 that enhances nDNA damage and repair responses. (regenerativemedicine.net)
  • Complejo heterodimérico de PROTEÍNA STAT1 y de PROTEÍNA STAT2 que interactúa con ISGF3GAMMA y es una subunidad del COMPLEJO ISGF3. (bvsalud.org)
  • A heterodimeric complex of STAT1 PROTEIN and STAT2 PROTEIN that interacts with ISGF3GAMMA and is a subunit of the ISGF3 COMPLEX. (bvsalud.org)
  • STAT1 and STAT2 are phosphorylated and bind to interferon regulatory factor 9 (IRF9) forming the complex known as interferon-stimulated gene factor 3 (ISGF3). (jcu.edu)
  • Target genes of the U-ISGF3 complex, consisting of unphosphorylated STAT1, STAT2, and IRF9 were highly enriched in the EMP-associated genes we identified. (ugent.be)
  • phosphorylated STAT1 and STAT2 bind IRF9 forming a complex named "IFN-Stimulated Gene Factor 3" (ISGF3). (wikidoc.org)
  • STAT1 is an indispensable component of a heterotrimer (ISGF3) and a STAT1 homodimer (GAF) that function as transcription regulators in type 1 and type 2 interferon signaling, respectively. (nih.gov)
  • In contrast, STAT1 recruitment to ISGF3 binding sites and type 1 interferon-dependent responses, including antiviral protection, remained intact. (nih.gov)
  • Further, an essential regulator of most ISGs is ISGF3 that is composed of STAT1, STAT2 and IRF9 complex and works in conjunction with IRFs. (tcr-signal.com)
  • These activated STAT molecules form heterodimers that translocate to the nucleus and associate with an additional factor, IFN regulatory factor-9 (IRF-9), forming the complex termed IFN-stimulated gene factor-3 (ISGF3). (jci.org)
  • Lastly, we aim to elucidate the mechanism of action of CDK-8 inhibitors shown to supress both U-ISGF3 target genes and EMP/EMT. (ugent.be)
  • 37. Time-dependent recruitment of GAF, ISGF3 and IRF1 complexes shapes IFNα and IFNγ-activated transcriptional responses and explains mechanistic and functional overlap. (nih.gov)
  • Experimental data of EMT-models in human colorectal cancer cell lines strongly supports such a role for U-ISGF3 signalling. (ugent.be)