About 3,600 dual specimens blood film samples for parasite diagnosis and filter paper blood spots for serology (using the immunofluorescence antibody test) were collected from the general population, including school populations, whenever possible. (biomedcentral.com)
These prob- mania antibodies have been described, lems could, theoretically, be avoided by de- including indirect immunofluorescence tecting antigen in the urine [ 20-22 ]. (who.int)
Antigen1
Results: Fifty-nine percent of NP had IgM antibodies against at least one CSP peptide and 87% against at least one Plasmodium vivax erythrocytic stage antigen. (bvsalud.org)
Serum1
Serum samples were evaluated by indirect fluorescent antibody test (IFAT cut-off titre ≥ 1:64), and brains were submitted to mouse bioassay. (scite.ai)
Seroprevalence2
High seroprevalence and antibody levels against blood-stage antigens were observed, which had a significant association with molecular evidence of infection. (bvsalud.org)
The aims of this study were to determine and identify factors associated to the seroprevalence of anti-Toxoplasma gondii antibodies in humans and dogs from Jataizinho, Parana State, Brazil. (scite.ai)
ELISA3
IgM antibodies against peptides of the circumsporozoite protein (CSP) from different Plasmodium species and different erythrocytic stage antigens were detected by ELISA. (bvsalud.org)
The prevalence of disease was determined by microscopic agglutination test (MAT), enzyme-linked<br />immunosorbent assay (ELISA), direct dark-field microscopic (DFM) examination, indirect fluorescent<br />antibody test (IFAT), microbiologic cultural isolation technique and polymerase chain reaction (PCR). (ac.ir)
Leptospiral antibodies were detected by ELISA in 85 sera (22.4%) among 380 samples. (ac.ir)
Malaria2
IgM antibodies against CSP and AMA-1 may be used as a potential marker for the identification of NP infected with Plasmodium, which are reservoirs of malaria in the Brazilian Atlantic Forest. (bvsalud.org)
The immunofluorescent antibody test (IFA) is a reliable and reproducible procedure for determining antibody levels for the indication of current or past infection with malaria [ 8 ]. (biomedcentral.com)
Diagnosis3
To reduce the four-day waiting time needed for diagnosis, a method using the indirect fluorescent antibody technique (IFAT) was developed, and, more recently, PCR-based methods have been developed. (wikipedia.org)
It seems that, to increase the accuracy in the diagnosis of the disease, using a<br />range of reliable techniques and comparing the results is important in reaching final conclusion. (ac.ir)
In the past, tion, the detection of antigens in patient se- many patients in Sudan died untreated in rum is complicated by the presence of high hospital, with the diagnosis of VL being levels of antibodies, circulating immune made later at autopsy [ 6 ]. (who.int)
Test3
An indirect fluorescent antibody test (IFAT) incorporating a polyclonal antibody to Marteilia sydneyi recognized spornlating stages of M. sydneyi from Saccostrea commercialis but not those of Marteilia refringens, M. maurini, Marteilia sp. (alr-journal.org)
KAtex est un simple supplément pour le diagnostic de la leishmaniose viscérale, en particulier sur le terrain, et un test complémentaire pour le diagnostic de la leishmaniose viscérale dans les cas à frottis négatif ayant des résultats positifs au test d'agglutination directe. (who.int)
Our results show that some horses sent to slaughter in Brazil have been exposed to T. gondii.Keywords: Slaughterhouse, horses, Toxoplasma gondii, bioassay, indirect fluorescent antibody test, nested PCR-RFLP. (scite.ai)
Parasite3
A seroepidemiological study of HCI in Israel revealed that 33% of the dogs surveyed had been exposed to the parasite as indicated by the presence of anti- H. canis antibodies. (vin.com)
Selon la technique d'immunogold, les anticorps polyclonaux spécifiques de M. sydneyi n'ont pas permis d'identifier le stade présporal présumé de ce parasite dans le tissu conjonctif d'un hôte récemment infecté. (alr-journal.org)
This study aimed to investigate anti-Toxoplasma gondii antibodies and to isolate the parasite from the brains of horses processed at slaughterhouses in Brazil. (scite.ai)
Sera2
lt;br />Antibodies were detected by MAT at least against one serovar of L. interrogans in 55 sera (14.5%) among<br />380 samples at a dilution of ≥ 1:100. (ac.ir)
For genotyping of the two isolates and the PCR-positive brain, we performed PCR-RFLP based on 13 markers, and SAG2 all samples were Toxoplasma gondii type I. Collectively, IFAT of horse sera and mouse bioassay identified positivity in 60 (15%) of the samples. (scite.ai)
Investigate1
Immunoelectron microscopy was used to investigate background labelling and the specificity of the antibody to antigenic sites. (alr-journal.org)
12.1%) of 33 urine samples were suspected by DFM examination and no positive sample by IFAT was<br />observed. (ac.ir)
In 12 of those 14 bioassays, mice were positive only by IFAT (cut-off titre ≥ 1:16), T. gondii being isolated in the remaining two. (scite.ai)
Study1
Therefore, in order to contribute to a better understanding of the immune response in different hosts and facilitate the identification of potential reservoirs, in this study, naturally acquired IgM antibody responses against Plasmodium antigens were evaluated, for the first time, in NP from the Atlantic Forest. (bvsalud.org)