To reduce the four-day waiting time needed for diagnosis, a method using the indirect fluorescent antibody technique (IFAT) was developed, and, more recently, PCR-based methods have been developed. (wikipedia.org)
The prevalence of disease was determined by microscopic agglutination test (MAT), enzyme-linked<br />immunosorbent assay (ELISA), direct dark-field microscopic (DFM) examination, indirect fluorescent<br />antibody test (IFAT), microbiologic cultural isolation technique and polymerase chain reaction (PCR). (ac.ir)
An indirect fluorescent antibody test (IFAT) incorporating a polyclonal antibody to Marteilia sydneyi recognized spornlating stages of M. sydneyi from Saccostrea commercialis but not those of Marteilia refringens, M. maurini, Marteilia sp. (alr-journal.org)
These prob- mania antibodies have been described, lems could, theoretically, be avoided by de- including indirect immunofluorescence tecting antigen in the urine [ 20-22 ]. (who.int)
Immunofluorescence1
About 3,600 dual specimens blood film samples for parasite diagnosis and filter paper blood spots for serology (using the immunofluorescence antibody test) were collected from the general population, including school populations, whenever possible. (biomedcentral.com)
Antigen1
Results: Fifty-nine percent of NP had IgM antibodies against at least one CSP peptide and 87% against at least one Plasmodium vivax erythrocytic stage antigen. (bvsalud.org)
ELISA2
IgM antibodies against peptides of the circumsporozoite protein (CSP) from different Plasmodium species and different erythrocytic stage antigens were detected by ELISA. (bvsalud.org)
Leptospiral antibodies were detected by ELISA in 85 sera (22.4%) among 380 samples. (ac.ir)
Malaria2
IgM antibodies against CSP and AMA-1 may be used as a potential marker for the identification of NP infected with Plasmodium, which are reservoirs of malaria in the Brazilian Atlantic Forest. (bvsalud.org)
The immunofluorescent antibody test (IFA) is a reliable and reproducible procedure for determining antibody levels for the indication of current or past infection with malaria [ 8 ]. (biomedcentral.com)
Diagnosis2
It seems that, to increase the accuracy in the diagnosis of the disease, using a<br />range of reliable techniques and comparing the results is important in reaching final conclusion. (ac.ir)
In the past, tion, the detection of antigens in patient se- many patients in Sudan died untreated in rum is complicated by the presence of high hospital, with the diagnosis of VL being levels of antibodies, circulating immune made later at autopsy [ 6 ]. (who.int)
Infection1
High seroprevalence and antibody levels against blood-stage antigens were observed, which had a significant association with molecular evidence of infection. (bvsalud.org)
Test1
KAtex est un simple supplément pour le diagnostic de la leishmaniose viscérale, en particulier sur le terrain, et un test complémentaire pour le diagnostic de la leishmaniose viscérale dans les cas à frottis négatif ayant des résultats positifs au test d'agglutination directe. (who.int)
Therefore, in order to contribute to a better understanding of the immune response in different hosts and facilitate the identification of potential reservoirs, in this study, naturally acquired IgM antibody responses against Plasmodium antigens were evaluated, for the first time, in NP from the Atlantic Forest. (bvsalud.org)
A seroepidemiological study of HCI in Israel revealed that 33% of the dogs surveyed had been exposed to the parasite as indicated by the presence of anti- H. canis antibodies. (vin.com)
Positive1
12.1%) of 33 urine samples were suspected by DFM examination and no positive sample by IFAT was<br />observed. (ac.ir)
Indirect fluorescent antibody test2
In our laboratory, we use the indirect fluorescent antibody test (IFAT) for antigens of the four plasmodia species, in addition to PCR screening. (cdc.gov)
38. Evaluation of an indirect fluorescent antibody test (IFAT) for demonstration of antibodies to Toxoplasma gondii in the sea otter (Enhydra lutris). (nih.gov)
Monoclonal2
Virus was identified by direct fluorescent antibody technique (DFAT) using FITC-conjugated anti-flavivirus antibody and serotyping was done by indirect fluorescent antibody technique (IFAT) using serotype-specific monoclonal anti-dengue antibody. (who.int)
Production, characterization and application of monoclonal antibodies against chicken IgY. (ijabbr.com)
Toxoplasma4
Objective: Sera from 186 sheep (93 rams and 93ewes) from Tabriz, North-West of Iran were evaluated for antibodies to Toxoplasma gondii using a commercial ELISA kit. (ijabbr.com)
Seroprevalence of anti-Toxoplasma gondii antibodies in buffaloes in Khoozestan province, Iran. (ijabbr.com)
Assays2
Survey of ovine and caprine toxoplasmosis by IFAT and PCR assays in Sardinia, Italy. (ijabbr.com)
Technologically advanced diagnostic assays and techniques, such as RTPCR, are experiencing high market demand over conventional diagnosis products. (dailybn.com)
Infections2
This review highlights the usefulness of diagnostic tools, such as the Baermann funnel technique, serology and PCR, and proposes to include these routinely on cases with clinical suspicion for lungworm infections. (biomedcentral.com)
Ces résultats pourraient servir de base rationnelle pour planifier une approche complète intégrée en matière de lutte contre les infections zoonosiques dans les zones enquêtées. (who.int)
Negative2
Moreover, 40% (50 of 125) of these sera were negative by IFAT (unpub. (cdc.gov)
these areas coincide with sera negative by IFAT). (cdc.gov)
Method1
The best diagnostic tool for the detection of A. vasorum or A. abstrusus first larvae in faeces is still represented by the Baermann funnel migration technique, which is unfortunately rarely utilized in small animal veterinary clinics of South America [ 19 ] even though it is a cheap and easy diagnostic method [ 20 ]. (biomedcentral.com)
Common1
High levels of IgG antibodies to HV were common, even in the acute phase of illness. (nih.gov)
Patients2
Over a 5-year period, a total of 35% of the NE suspected patients revealed antibodies to HV, but this varied considerably in the different years. (nih.gov)
Antibodies to HV were detected in 74% male and 26% female NE patients, but the ratio varied between age groups. (nih.gov)