• Hypoxia-inducible factor prolyl hydroxylase inhibition: Robust new target or another big bust for stroke therapeutics? (wikipedia.org)
  • We have previously shown that von Hippel-Lindau (VHL) regulates ubiquitylation and proline 1465 hydroxylation of the large subunit of RNA polymerase II, Rpb1, in human renal clear cell carcinoma (RCC) cell lines. (nih.gov)
  • Here, our goal was to determine the effect of this VHL function and the status of P1465 hydroxylation in human RCC tumors.Primary human tumors and matched normal kidney samples were probed for expression levels of the large subunit of RNA polymerase II (Rpb1), Rpb1 hydroxylated on P1465 [Rpb1(OH)], Rpb1 phosphorylated on Ser5 [Rpb1(S5P)], and proline hydroxylases PHD1, PHD2, and PHD3. (nih.gov)
  • In hypoxia, FIH-1 hydroxylation of Asn803 within the C-terminal transactivation domain does not occur and HIF-1α forms a fully active transcriptional complex. (biomedcentral.com)
  • Nickel depletes intracellular ascorbate, which leads to the inhibition of cellular hydroxylases, manifested by the loss of hypoxia-inducible factor (HIF)-1α and - 2α hydroxylation and hypoxia-like stress. (nih.gov)
  • Proline hydroxylation is crucial for collagen and extracellular matrix assembly as well as for assembly of other protein molecules that have collagen-like domains, including surfactants and complement. (nih.gov)
  • The hydroxylation of proteins occurs as a post-translational modification, and is catalyzed by 2-oxoglutarate-dependent dioxygenases. (wikipedia.org)
  • [9] Proline hydroxylation is also a vital component of hypoxia response via hypoxia inducible factors . (wikipedia.org)
  • Deprivation of ascorbate leads to deficiencies in proline hydroxylation, which leads to less stable collagen, which can manifest itself as the disease scurvy . (wikipedia.org)
  • These interventions maintained hydroxylation of HIF-1α protein and prevented stabilization of HIF-1α protein during hypoxia. (rupress.org)
  • Antioxidants maintained hydroxylation of HIF-1α protein and prevented stabilization of HIF-1α protein during hypoxia. (rupress.org)
  • Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. (thermofisher.com)
  • Hypoxia-inducible factor-proline dioxygenase (EC 1.14.11.29, HIF hydroxylase) is an enzyme with systematic name hypoxia-inducible factor-L-proline, 2-oxoglutarate:oxygen oxidoreductase (4-hydroxylating). (wikipedia.org)
  • The neoplastic cells can adapt to this hostile microenvironment using the activation of hypoxia-induced genes for angiogenesis, glycolysis and other processes advantageous to cell proliferation and survival. (biomedcentral.com)
  • The activation of these hypoxia-induced genes centres on the levels of hypoxia-inducible factor (HIF) 1 within the tumour cell [ 3 ]. (biomedcentral.com)
  • Both genes encode major components of the hypoxia-inducible factor (HIF) transcriptional pathway, which coordinates an organism's response to hypoxia. (ox.ac.uk)
  • Among the key regulatory molecules that have been reported to be involved in metabolic shift so far, hypoxia-inducible factor 1 (HIF1) controls the transcription of many target genes to initiate metabolic changes in the early stage and maintains glycolytic metabolism in the later phase of reprogramming. (biomedcentral.com)
  • Mammalian cells increase transcription of genes for adaptation to hypoxia through the stabilization of hypoxia-inducible factor 1α (HIF-1α) protein. (rupress.org)
  • Transcriptional activity is selectively and progressively dampened at bivalently marked loci upon repeated exposure to hypoxia, indicating that this subset of genes uniquely maintains the potential for epigenetic regulation by KDM activity. (biomedcentral.com)
  • This enzyme catalyses the following chemical reaction hypoxia-inducible factor-L-proline + 2-oxoglutarate + O2 ⇌ {\displaystyle \rightleftharpoons } hypoxia-inducible factor-trans-4-hydroxy-L-proline + succinate + CO2 Hypoxia-inducible factor-proline dioxygenase contains iron, and requires ascorbate. (wikipedia.org)
  • Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. (thermofisher.com)
  • It can take place on several amino acids, like lysine, asparagine, aspartate and histidine, but the most frequently hydroxylated amino acid residue in human proteins is proline . (wikipedia.org)
  • Their concerted action affects access of transcriptionally regulatory proteins to chromatin by nucleosome repositioning, recruitment of regulatory factors (i.e., scaffolding factors) and/or altered chromatin compaction. (biomedcentral.com)
  • Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. (thermofisher.com)
  • His work in the fields of Drosophila Protein overlaps with other areas such as Procollagen-proline dioxygenase. (research.com)
  • Paul Vigne spends much of his time researching Fusion protein, Green fluorescent protein, Molecular biology, Procollagen-proline dioxygenase and Biochemistry. (research.com)
  • His Fusion protein research incorporates themes from Cyclosporin a, Hypoxia-inducible factors and Peptide. (research.com)
  • We demonstrate that cells deficient in the complex III subunit cytochrome b , which are respiratory incompetent, increase ROS levels and stabilize the HIF-1α protein during hypoxia. (rupress.org)
  • protein_coding" "Cz03g38230.t1","No alias","Chromochloris zofingiensis","Glyoxalase/fosfomycin resistance/dioxygenase domain [Interproscan]. (ntu.edu.sg)
  • They are OXYGEN-dependent enzymes that play an important role in mediating cellular adaptive responses to HYPOXIA . (nih.gov)
  • In humans, there are three isoforms of hypoxia-inducible factor-proline dioxygenase. (wikipedia.org)
  • Transcriptional changes in hypoxic cancer cells are controlled by several well-understood hypoxia response pathways [ 12 - 14 ]. (biomedcentral.com)
  • Tibetans living at sea level have a hyporesponsive hypoxia-inducible factor system and blunted physiological responses to hypoxia. (ox.ac.uk)
  • EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. (thermofisher.com)
  • Induced pluripotent stem cells (iPSCs) were first established from differentiated somatic cells by gene introduction of key transcription factors, OCT4, SOX2, KLF4, and c-MYC, over a decade ago. (biomedcentral.com)
  • MsATG13 transgenic tobacco also increased the proline contents and antioxidant enzyme activities, enhancing the antioxidant defense capabilities under cold stress conditions. (bvsalud.org)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Brain Derived Neurotrophic Factor (BDNF) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. (scalegen.com)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Brain Derived Neurotrophic Factor (BDNF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species. (scalegen.com)
  • As oxygen levels decrease in the surrounding environment (hypoxia), cells respond by activating hypoxia- inducible factor (HIF) dependent gene transcription to facilitate cellular adaptation to hypoxia. (rupress.org)
  • Hypoxia-induced bivalency substantially overlaps with embryonal stem cell-associated genic bivalency and is retained at numerous loci upon reoxygenation. (biomedcentral.com)
  • We found that Tibetans had a lower hemoglobin concentration, a higher pulmonary ventilation relative to metabolism, and blunted pulmonary vascular responses to both acute (minutes) and sustained (8 h) hypoxia. (ox.ac.uk)
  • Hypoxia-inducible factor 1 (HIF-1) functions as a master regulator of oxygen homeostasis in metazoan species. (johnshopkins.edu)
  • Mechanistically, KGDHC dysfunction causes increased demethylation of nuclear DNA by α-KG-dependent dioxygenases (e.g. (biomed.news)
  • Description: A sandwich quantitative ELISA assay kit for detection of Human Brain Derived Neurotrophic Factor (BDNF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. (scalegen.com)
  • Induced pluripotent stem cells (iPSCs) are established by the introduction of reprogramming factors (OCT4, SOX2, KLF4, and c-MYC) into somatic cells. (biomedcentral.com)
  • Hypoxia-inducible factor (HIF)-1α levels in invasive breast carcinoma have been shown to be an adverse prognostic indicator. (biomedcentral.com)
  • We sought to test the hypothesis that natural selection to hypoxia within Tibetans results in related phenotypic differences. (ox.ac.uk)
  • The present study investigates the role of FIH-1 in invasive breast carcinoma and its correlation with hypoxia. (biomedcentral.com)
  • Cytoplasmic FIH-1 expression was also an independent poor prognostic factor for disease-free survival. (biomedcentral.com)
  • After drought stress treatment, the electrolyte leakage and malondialdehyde content of transgenic tobacco were lower than those of wild-type tobacco, but the proline content and soluble sugar content were higher than those of wild-type tobacco. (bvsalud.org)
  • Hypoxia-inducible factor (HIF) is an evolutionarily conserved transcription factor that allows the cell to respond physiologically to low concentrations of oxygen. (wikipedia.org)
  • Its members are X factor (HEMIN) independent and variably dependent on V factor (NAD). (nih.gov)