Hydrolysis beta glucosidase. Medical search. Frequent questions

In situ soil zymography showed that the distribution of C-acquiring enzymes (β-glucosidase and cellobiohydrolase) was more associated with root-detritus than P-acquiring enzymes (acid and alkaline phosphomonoesterase). (springer.com)
Cellulases are a group of enzymes, which catalyse different steps of cellulose hydrolysis, and are broadly used in industry as unpurified mixtures of several enzymes. (springeropen.com)
The name "cellulase" is commonly used for a natural mixture of enzymes that in general includes three major glycosylases: endoglucanase (EC 3.2.1.4), exoglucanase (cellulase 1,4-beta-cellobiosidase (non-reducing end), EC 3.2.1.91), and cellobiase or β- d -glucosidase (EC 3.2.1.21) (Anoop Kumar et al. (springeropen.com)
β-Glucosidases finalize the process by hydrolyzing cellobiose into glucose, so the efficiency of cellulose hydrolysis largely depends on the quantity and quality of these enzymes used during saccharification. (biomedcentral.com)
beta-Glucosidases are enzymes that participate in the hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose. (innexscientific.com)
The SalA and SalB enzymes preferentially hydrolyzed aryl beta-glucosides. (lookformedical.com)
The enzymes employed, namely exo -inulinase and endo -inulinase, give incompletely hydrolysis of levan. (megazyme.com)
Furthermore, A. awamori produced ferulic acid esterase (160 IU/L) which acts synergistically with cellulolytic-xylanolytic enzymes in the hydrolysis of lignocellulosic materials. (microrna21.com)
Moreover, the hydrolytic effectiveness of the blends was superior to the use of unblended T. reesei or A. awamori enzymes, under corresponding conditions (10 FPU/g bagasse, 20 g bagasse/L and 50 degrees C). Hydrolysis experiments, presenting either 20 or 200 g/L bagasse, resulted in 3.9 or 40g glucose/L, respectively. (microrna21.com)
The β-glucosidase enzymes are widespread in microorganisms, and their genes of production can differ in strains of the same species [ 9 ]. (springeropen.com)
Glucosyltransferase and glucosidase are performed by a single enzyme in mammals, yeast, and some bacteria, but by two distinct enzymes in E. coli and other bacteria, complicating nomenclature. (wikidoc.org)
When glucosyltransferase and glucosidase are catalyzed by distinct enzymes, "glycogen debranching enzyme" usually refers to the glucosidase enzyme . (wikidoc.org)
Thus the debranching enzymes, transferase and α-1,6- glucosidase converts the branched glycogen structure into a linear one, paving the way for further cleavage by phosphorylase. (wikidoc.org)
In E. coli and other bacteria, glucosyltransferase and glucosidase functions are performed by two distinct enzymes. (wikidoc.org)
The recalcitrant nature of cellulosic materials and the high cost of enzymes required for efficient hydrolysis are the major impeding steps to their practical usage for ethanol production. (biomedcentral.com)
Unfortunately, the recalcitrant nature of cellulosic materials and the high cost of enzymes required for efficient hydrolysis are the major limiting steps to the more widespread exploitation of this natural resource [ 3 ]. (biomedcentral.com)
Enzymes, particularly ss-glucosidase and al-arabinofuranosidase, were able to tolerate NaCl up to a final concentration of 12% (v/w). (omu.edu.tr)
It is achieved by a simultaneous action of three enzymes: endo- β -glucanase (EC 3.2.1.4), exo- β -glucanase (EC 3.2.1.91), and β -glucosidase (EC 3.2.1.21) [ 5 ]. (hindawi.com)
4-Nitrophenyl beta-D-cellobioside (p-nitrophenyl-β-D-cellobioside or PNPC) is a synthetic substrate used to measure the activity of cellobiases, enzymes that hydrolyze cellobiose to glucose monomers. (chemsynlab.com)

The total activity of cellulase is defined as the ability of the enzyme to produce glucose, which is the final product of cellulose hydrolysis, and is expressed in cellulase units. (springeropen.com)
However, common strategies for the determination of the cellulolytic activity of industrial cellulase preparations are based on the assessment of different steps of cellulose hydrolysis, and the results obtained with different methods are not similar. (springeropen.com)
The aim of the present study was to develop an assay for the determination of cellulase activity that relies on the amperometric determination of the final product of cellulose hydrolysis glucose. (springeropen.com)
2019 ). The enzyme mixture acts synergistically on the hydrolysis of cellulose which is gradually broken down from crystalline and/or amorphous cellulose to smaller, soluble fragments and finally to glucose (Dimarogona et al. (springeropen.com)
During cellulose hydrolysis, endoglucanases first attack the amorphous area of the polysaccharide chain, breaking the β-1,4-glycosidic bonds releasing cellodextrin and creating new chain ends. (springeropen.com)
During cellulose saccharification, large amounts of β-glucosidases must be added to avoid feedback inhibition of exo-glucanases and endo-glucanases by cellobiose and to compensate for the loss of β-glucosidase catalysis efficiency due to accumulation of both cellobiose and glucose [ 7 ]. (biomedcentral.com)
The potential for significant environmental benefits from lignocellulosic ethanol production via enzymatic cellulose hydrolysis have not yet been realized, in large part because of high enzyme production costs when using microbial protein production systems. (cornell.edu)
Obtained from the partial hydrolysis of cellulose. (lookformedical.com)
Instead, the substantial increase in ethanol production is consistent with the reported synergistic effect on cellulose hydrolysis using the displayed mini-cellulosome. (biomedcentral.com)
Bioethanol production requires four major steps: biomass pretreatment, cellulose hydrolysis, fermentation, and distillation [ 3 ]. (hindawi.com)
Cellulose hydrolysis to glucose could be performed by chemical, physical, or enzymatic processes. (hindawi.com)

Beta-D-glucopyranosyl abscisate beta-glucosidase (EC 3.2.1.175, AtBG1, ABA-beta-D-glucosidase, ABA-specific beta-glucosidase, ABA-GE hydrolase, beta-D-glucopyranosyl abscisate hydrolase) is an enzyme with systematic name beta-D-glucopyranosyl abscisate glucohydrolase. (wikipedia.org)
This enzyme catalyses the following chemical reaction D-glucopyranosyl abscisate + H2O ⇌ {\displaystyle \rightleftharpoons } D-glucose + abscisate The enzyme hydrolyzes the biologically inactive beta-D-glucopyranosyl ester of abscisic acid to produce active abscisate. (wikipedia.org)
With this model, we were able to show that under almost all market conditions, the use of beta glucosidase, an expensive enzyme, resulted in lower-cost production of the fuel ethanol. (usda.gov)
2008). In this process, in addition to beta-glucanases, which is added to reduce the viscosity of the barley mash for efficient mixing, another enzyme, beta-glucosidase, is also added to completely hydrolyze the glucose oligomers obtained during the hydrolysis of beta-glucans in barley grains by beta-glucanases to the fermentable sugar glucose. (usda.gov)
Levan hydrolysis requires a third enzyme, endo -levanase. (megazyme.com)
The enzyme profile of T. reesei gave (IU/L): 1700 FPA, 20,000 CMCase, 340 beta-glucosidase and 12,600 xylanase. (microrna21.com)
Oleuropein, the main bitter phenolic glucoside responsible for green olive bitterness, may be degraded by the β-glucosidase enzyme to release glucose and phenolic compounds. (springeropen.com)
However, the β-glucosidase was activated with an increase of NaCl concentration, with a maximum activity obtained at 8% NaCl (w/v). The enzyme activity was optimal at pH 5 for both strains, while the optimum temperature was 45 °C for L. plantarum FSO1 and 35 °C for C. pelliculosa L18. (springeropen.com)
L. plantarum FSO1 and C. pelliculosa L18 strains showed their ability to produce an extracellular and induced β-glucosidase enzyme with promising traits for application in the biological processing of table olives. (springeropen.com)
A debranching enzyme is a molecule that helps facilitate the breakdown of glycogen , which serves as a store of glucose in the body, through glucosyltransferase and glucosidase activity. (wikidoc.org)
Hydrolysis of pretreated wheat straw using the crude enzyme from J. alba DSM 45237 released 160.9 mg/gds reducing sugars. (omu.edu.tr)

In the final step, β-glucosidases cleave exocellulase products releasing soluble individual monosaccharides such as glucose. (springeropen.com)
β-Glucosidases (EC 3.2.1.21) finalize the cellulolytic process by hydrolyzing cellobiose into glucose. (biomedcentral.com)
A disaccharide consisting of two glucose units in beta (1-4) glycosidic linkage. (lookformedical.com)
Quantification is based on complete hydrolysis of fructan to fructose and glucose by a mixture of exo -inulinase, endo -inulinase, and endo -levanase, followed by measurement of these sugars using the PAHBAH reducing sugar method which gives the same color response with fructose and glucose. (megazyme.com)
These agents lower postprandial glucose by slowing glucose absorption and delaying the hydrolysis of ingested complex carbohydrates and disaccharide. (medscape.com)
Amylo-α-1,6-glucosidase ( EC 3.2.1.33 ), or glucosidase , cleaves the remaining alpha-1,6 linkage, producing glucose and a linear chain of glycogen. (wikidoc.org)
After volume and pH adjustments and filtration, the solution is treated with β-glucosidase, which hydrolyzes β-gluco-oligosaccharides to D-Glucose. (megazyme.com)
4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose. (systemsbiology.net)

Recent research has aimed to improve the quality of lignocellulosic feedstock, pretreatment processes, and the efficiency of enzymatic hydrolysis to reduce biofuel production costs [ 2 , 3 ]. (biomedcentral.com)
Enzymatic hydrolysis is the most used in the industry because of its high yields with fewer unwanted side products [ 4 ]. (hindawi.com)
Enzymatic Hydrolysis of Human Milk Oligosaccharides. (iqs.edu)

The F256 substrate-binding residue in D2-BGL is located in a shorter loop surrounding the active site pocket relative to that of Aspergillus β-glucosidases, and this short loop is responsible for its high substrate affinity toward cellobiose. (biomedcentral.com)
The rhizosphere nitrogen-fixing bacterium Azospirillum irakense KBC1 is able to grow on pectin and beta-glucosides such as cellobiose, arbutin, and salicin. (lookformedical.com)
The transformants were able to ferment not only arbutin, but also cellobiose, salicin and methyl-beta-glucoside (MbetaGlc). (lookformedical.com)
PNPC can be analyzed through enzymatic assays that monitor the rate of cellobiose hydrolysis using spectrophotometric techniques. (chemsynlab.com)
PNPC has been widely used as a substrate for measuring cellobiase activity and studying the mechanisms of cellobiose hydrolysis. (chemsynlab.com)
For example, PNPC is not an ideal substrate for all cellobiases and may not accurately reflect in vivo cellobiose hydrolysis. (chemsynlab.com)
The use of PNPC and related substrates to study the roles of cellobiases and β-glycosides in biological systems beyond cellobiose hydrolysis. (chemsynlab.com)

The β-glucosidase, the exoglucanase and the endoglucanase activity of the trifunctional cellulase BCE reached 16.80 IU/mg, 2.26 IU/mg and 20.67 IU/mg, which was 46.27, 6.73 and 46.20% higher than the activities of the β-glucosidase BG, the endoglucanase CBH and the endoglucanase EG. (biomedcentral.com)
The trifunctional cellulase BCE was designed based on β-glucosidase BG, endoglucanase EG and exoglucanase CBH, and it possessed β-glucosidase activity, endoglucanase activity and exoglucanase activity simultaneously. (biomedcentral.com)
The characterization and optimization of cellulase activities are carried out on a yeast isolate that belongs to the genus Trichosporon , which was the most powerful with regard to the hydrolysis halo of CMC diameter, in a qualitative test. (hindawi.com)

The Thermobifida fusca cel6A and bglC genes, encoding an endoglucanase and a Beta-glucosidase, respectively, were inserted into the Nicotiana tabacum chloroplast for expression with various 14-amino acid downstream box (DB) fusions added to the N-terminus of each protein. (cornell.edu)
A yeast consortium composed of four different populations was engineered to display a functional mini-cellulosome containing an endoglucanase, an exoglucanase and a β-glucosidase. (biomedcentral.com)
It has been used to characterize the function of endoglucanase 1 from T. harzianum, β-glucosidase from T. xylanolyticum, and glycoside hydrolase family 3 (GH3) aryl β-glucosidases from A. oryzae. (chemsynlab.com)

A sensitivity study was also performed to examine the effects of alpha-glucosidase and barley costs on the final ethanol production cost. (usda.gov)
Antioxidant rich grape pomace extract suppresses postprandial hyperglycemia in diabetic mice by specifically inhibiting alpha-glucosidase, 1-9. (ugm.ac.id)

The results showed that strains produced extracellular and induced β-glucosidase, with a molecular weight of 60 kD. (springeropen.com)
Jiangella alba DSM 45237 exhibited excellent extracellular ss-glucosidase (1.03 +/- 0.09 U/mL), ss xylosidase (16.29 +/- 0.23 U/mL), and alpha-l-arabinofuranosidase (7.00 +/- 0.09 U/mL) production in the growth media containing 15 g/L wheat straw pretreated with NaOH. (omu.edu.tr)

The Beta-Glucan test kit is suitable for the measurement and analysis of Beta-Glucan (Mixed Linkage). (megazyme.com)

We heterologously expressed the fungal β-glucosidase D2-BGL from a Taiwanese indigenous fungus Chaetomella raphigera in Pichia pastoris for constitutive production by fermentation. (biomedcentral.com)
Fortunately, deglycosylation of isoflavones can be achieved during fermentation process by several strains such as lactic acid bacteria, basidiomycetes, filamentous fungus, and Bacillus subtilis with their β-glucosidase activity. (scite.ai)

Two adjacent genes, salA and salB, conferring beta-glucosidase activity to Escherichia coli, have been identified in a cosmid library of A. irakense DNA. (lookformedical.com)
Lactobacillus plantarum FSO1 and Candida pelliculosa L18 strains, isolated from natural fermented green olives, were tested for their β-glucosidase production and activity at different initial pH, NaCl concentrations, and temperature. (springeropen.com)
However, the β-glucosidase activity of L. plantarum strain obtained was lower in the olive brine environment and led to the production of a fermented green olive with some bitterness [ 16 ]. (springeropen.com)
The conditions of production and activity of β-glucosidase of Lactobacillus and yeast strains, particularly in olive brine conditions, are not well highlighted. (springeropen.com)
glucosidase activity of bifidobacteria and the hydrolysis of isoflavone glycosides by Bifidobacterium sp. (ugm.ac.id)
Among solvents, only ethanol and methanol increased the ss-glucosidase activity. (omu.edu.tr)

Identification of cellulolytic microorganisms is of great interest to the hydrolysis of cellulosic biomass. (hindawi.com)

Lower ethanol production cost was obtained compared to that obtained without beta-glucosidase addition in all cases except one where highest beta-glucosidase cost allowance and lowest barley cost were used. (usda.gov)
Results indicated that the slight difference in cell growth cannot explain the 3-fold increase in PASC hydrolysis and ethanol production. (biomedcentral.com)

This is the first report related to the characterization of ss-glucosidase, ss-xylosidase, and alpha-l-arabinofuranosidase from J.alba DSM 45237 to date. (omu.edu.tr)

[7] This is a common method through which to cleave bonds, with an acid below the site of hydrolysis to lend a proton and a base above to deprotinate a water which can then act as a nucleophile . (wikidoc.org)

Analysis of hydrolysis products with thin layer chromatography (TLC) showed that major products were 5C sugars. (omu.edu.tr)

The genes encoding three alpha-glucosidases RPMI00511, RPMI00720 and RPMI01263 from P. miotherma were cloned and characterized. (uni-goettingen.de)

This is the first genetic implication of this beta-glucosidase family in the utilization of beta-glucosides for microbial growth. (lookformedical.com)
Microbial beta-Glucosidase: Cloning, Properties, and Applications. (ugm.ac.id)

The economical benefits of adding beta-glucosidase significantly increase with decreases in beta-glucosidase cost and increases in barley cost. (usda.gov)

However the beta-glucosidase and xylanase activities PP2 cost were 134- and 6-fold lower than those of A. awamori (45,600 and 79,100 IU/L, respectively). (microrna21.com)

Growth of Azospirillum irakense KBC1 on the aryl beta-glucoside salicin requires either salA or salB. (lookformedical.com)

The first step involves the removal of one of the two -D-gluco pyranosyl groups from amygdalin through the action of beta-glucosidase to form the cyanogenic glycoside, prunasin. (cdc.gov)

B . Using allylglucosinolate ( 4 , aliphatic side chain with terminal double bond) as an example, the hydrolysis by myrosinase ( a ) is shown that leads to the formation of an isothiocyanate upon spontaneous rearrangement of the aglycone ( b ) or to the formation of alternative products ( c - e ). (biomedcentral.com)

Expression of the phospho-beta-glycosidase ArbZ from Lactobacillus delbrueckii subsp. (lookformedical.com)
The arbZ gene was cloned and expressed in the industrially used beta-glucoside-negative strain Lactobacillus helveticus 3036(62). (lookformedical.com)

At neutral pH, cyanogen undergoes a slow hydrolysis to form hydrogen cyanide, cyanic acid (HOCN), and other products. (cdc.gov)
Involvement of acid beta-glucosidase 1 in the salvage pathway of ceramide formation. (musc.edu)
Identification of arachidonic acid as a mediator of sphingomyelin hydrolysis in response to tumor necrosis factor alpha. (musc.edu)

[5] The mechanism by which the glucosidase cleaves the α -1,6-linkage is not fully known because the amino acids in the active site have not yet been identified. (wikidoc.org)
NMR-spectroscopic examinations revealed that the hydrolysis follows a retention mechanism at the anomeric center. (uni-goettingen.de)

The chemical composition of U.S.-patented Laetrile (mandelonitrile-beta-glucuronide), a semisynthetic derivative of amygdalin, is different from the laetrile/amygdalin produced in Mexico (mandelonitrile beta-D-gentiobioside), which is made from crushed apricot pits. (cancer.gov)

The optimum temperature was 40 degrees C for ss-glucosidase and ss-xylosidase, whereas it was 50 degrees C for a-larabinofuranosidase. (omu.edu.tr)
The optimum temperature for pNPG hydrolysis was 40 °C, and complete reaction can be reached in 30 min. (scite.ai)

Tolbutamide increases insulin secretion from pancreatic beta cells. (medscape.com)

The increase of NaCl concentration, from 0 to 10% (w/v), inhibited the production of β-glucosidase for both strains. (springeropen.com)
After 4 lactamase production in a Salmonella en- days of incubation, bacterial growth terica serotype Typhi strain from the Phil- ippines. (cdc.gov)

Our structure analysis of D2-BGL offers new insights into GH3 β-glucosidases, which will be useful for strain improvements via a structure-based mutagenesis approach. (biomedcentral.com)

Ketone bodies are largely beta-hydroxybutyrate and acetoacetate. (medscape.com)

This motife has similarity to a conserved sequence motife of the Rossman-Fold which is a conserved structural region for the binding of cofactors in proteins and consists of an alternating sequence of beta sheets and alpha helices. (uni-goettingen.de)

The drug's action depends on functional beta cells in pancreatic islets. (medscape.com)

lactis was previously shown to enable utilization of the beta-glucoside arbutin by Escherichia coli. (lookformedical.com)

These three hydrolysis steps can occur simultaneously (Percival Zhang et al. (springeropen.com)

Glipizide is a second-generation sulfonylurea that stimulates the release of insulin from pancreatic beta cells. (medscape.com)
Stimulates insulin release from pancreatic beta cells. (medscape.com)

Chlorpropamide may increase insulin secretion from pancreatic beta cells. (medscape.com)

Anatomy1

Organisms1

Diseases2

Chemicals and Drugs26

Phenomena and Processes8

Information Science3

Enzymes19

Cellulose11

Enzyme11

Glucose8

Enzymatic hydrolysis3

Cellobiose7

Cellulase3

Endoglucanase3

Alpha-glucosidase2

Extracellular2

Glucan1

Fermentation2

Activity6

Biomass1

Ethanol2

Characterization1

Cleave1

Sugars1

Genes1

Microbial2

Barley1

Xylanase1

Aryl1

Glycoside1

Terminal1

Lactobacillus2

Acid3

Mechanism2

Laetrile1

Optimum temperature2

Increases1

Production2

Strain1

Largely1

Proteins1

Depends1

Utilization1

Simultaneously1

Release2

Increase1