• Scaffolds have been utilized in tissue regeneration to facilitate the formation and maturation of new tissues or organs where a balance between temporary mechanical support and mass transport (degradation and cell growth) is ideally achieved. (hindawi.com)
  • One of the most attractive subjects in tissue engineering is the development of a scaffold, a three-dimensional porous solid structure that plays a key role in assisting tissue regeneration [ 1 ]. (hindawi.com)
  • Its degradation rate should match the rate of new tissue regeneration in order to maintain the structural integrity and to provide a smooth transition of the load transfer from the scaffold to the tissue [ 3 ]. (hindawi.com)
  • Biodegradability can be imparted into polymers through molecular design with a controlled rate in concert with tissue regeneration [ 18 - 21 ]. (hindawi.com)
  • Recently, carbon-modified biomaterials for bone tissue engineering have been extensively investigated to potentially revolutionize biomaterials for bone regeneration. (openorthopaedicsjournal.com)
  • This review summarizes the chemical and biophysical properties of carbon nanostructures and discusses their functionality in bone tissue regeneration. (openorthopaedicsjournal.com)
  • In the quest for alternative therapeutic strategies, research is focusing on the concept of tissue engineering to assist the progress of bone tissue regeneration. (openorthopaedicsjournal.com)
  • Convergence of scaffold-guided bone regeneration principles and microvascular tissue transfer surgery. (isbweb.org)
  • To this effect, Poly-Med offers Max-Prene® 955 and Lactoprene® 100M, which both exhibit an elastic modulus suitable for hard tissue regeneration, with values in the range of 63-89 MPa. (poly-med.com)
  • Clinical applications of our research involves wound healing, cardiac tissue regeneration, ocular disease, and cancer imaging and therapy. (stanford.edu)
  • Electrospun nanocomposite for bone tissues regeneration via osteoconduction, osteoinduction, and osteogenesis. (dokumen.pub)
  • Fang Z, Starly B, Sun W (2005) Computer-aided characterization for effective mechanical properties of porous tissue scaffolds. (springer.com)
  • Porous scaffold architecture guides tissue formation. (mpg.de)
  • Polymers have been widely chosen as tissue scaffolding material having a good combination of biodegradability, biocompatibility, and porous structure. (hindawi.com)
  • With further optimization in topologically ordered porosity design exploiting material property and fabrication technique, porous biodegradable metals could be the potential materials for making hard tissue scaffolds. (hindawi.com)
  • Ideally, a scaffold must be porous, bioactive, and biodegradable and possess adequate mechanical properties suited to the biological site. (hindawi.com)
  • Zhang and Ma have developed [ 25 ] a highly porous biodegradable polymer/apatite composite scaffold (95% porosity) through a thermally induced phase separation technique, which resulted in significant improvement in mechanical properties compared to polymer-only scaffold. (hindawi.com)
  • Tamaddon M, Samizadeh S, Wang L, Blunn G, Liu C. Intrinsic Osteoinductivity of Porous Titanium Scaffold for Bone Tissue Engineering. (scielo.br)
  • In this study, porous scaffold materials based on polyvinyl alcohol (PVA) and gelatin (Gel) were successfully fabricated and characterized. (mdpi.com)
  • These osteoconductive three dimensional constructs seeded with MSCs are highly porous, biodegradable and biomechanically stable scaffolds which do not evoke an immunogenic host cell response. (openorthopaedicsjournal.com)
  • These scaffolds are usually porous and often harbor various biomolecules and/or cells. (ceramic-science.com)
  • The 3D-printed scaffold structures are used as supports in replacing and repairing fractured bone tissue. (mdpi.com)
  • The biomaterial scaffold design should consider physicochemical properties, microstructure, and degradation in vivo . (openorthopaedicsjournal.com)
  • 2010) Coating of biomaterial scaffolds with the collagen-mimetic peptide GFOGER for bone defect repair. (upstate.edu)
  • 20) C Li, L Ouyang, IJ Pence, AC Moore, Y Lin, CW Winter, JPK Armstrong* & MM Stevens* "Buoyancy-driven gradients for biomaterial fabrication and tissue engineering" Advanced Materials 1900291 (2019). (thearmstronggroup.co.uk)
  • The latest publication, authored by Mina Mohseni, Professor Dietmar Hutmacher, and Dr. Nathan Castro, highlights performance of these filaments in fused filament fabrication (FFF) additive manufactured (AM) tissue scaffolding. (poly-med.com)
  • pp. 312-342) edited by Dietmar Hutmacher and Wojciech Chrzanowski. (edu.au)
  • Metals that can degrade in physiological environment, namely, biodegradable metals, are proposed as potential materials for hard tissue scaffolding where biodegradable polymers are often considered as having poor mechanical properties. (hindawi.com)
  • Biodegradable metal scaffolds have showed interesting mechanical property that was close to that of human bone with tailored degradation behaviour. (hindawi.com)
  • A biodegradable scaffold allows the replacement of biological tissues via physiological extracellular components without leaving toxic degradation products. (hindawi.com)
  • Biodegradable polymers have been widely used and accepted as the most suitable materials for scaffolds due to their degradability, biocompatibility, and ease of processability [ 9 - 11 ]. (hindawi.com)
  • Biodegradable polymers degrade through hydrolysis process and are gradually absorbed by the human body thus allowing the supported tissue to gradually recover its functionality [ 8 , 17 ]. (hindawi.com)
  • Synthetic polymers have been used in medical devices for more than five decades, and key recent advances have largely focused on the development of biodegradable (or resorbable) materials for tissue engineering 7 . (nature.com)
  • Three-dimensional tissue growth in polymer scaffolds with different stiffness and in-vitro influence of BMP-2 on tissue formation in hydroxyapatite scaffolds. (mpg.de)
  • Two stages in three-dimensional in vitro growth of tissue generated by osteoblastlike cells. (mpg.de)
  • Gradual conversion of cellular stress patterns into pre-stressed matrix architecture during in vitro tissue growth. (mpg.de)
  • For instance, PLA could be combined with PGA to form poly(lactic- co -glycolic acid) (PLGA), which has degradation rate tailored with the tissue healing period and has been shown to support osteoblast cells attachment and growth in vitro and in vivo [ 22 - 24 ]. (hindawi.com)
  • These approaches can be developed using biomaterials and growth factors that seek to induce tissue formation through stimulating specific cellular functions in vitro and in vivo [1,2]. (tissuelabs.com)
  • 2017) Towards Combining Cell Printing and CRISPR Epigenome Editing for Engineered IVD and Musculoskeletal Tissues, Biomedical Engineering Society Annual Meeting, Podium Presentation, Phoenix, AZ. (utah.edu)
  • Printed bone scaffolds with complex structures produced using additive manufacturing technology can mimic the mechanical properties of natural human bone, providing lightweight structures with modifiable porosity levels. (mdpi.com)
  • Three groups of scaffolds with increasing levels of porosity, and pore sizes of 490 ± 10 μm, were fabricated and evaluated for compressive properties. (springer.com)
  • Results indicate that this technique allows the fabrication of scaffolds with high amount of interconnectivity and controllability of porosity, pore size, and mechanical properties, the advantage that other methods lack. (springer.com)
  • Sufficient porosity is needed to accommodate cell proliferation and differentiation, which will eventually enhance tissue formation [ 2 , 3 ]. (hindawi.com)
  • The authors highlighted the importance of combining different types of materials to meet the requirements of the tissue in terms of porosity, surface area, and mechanical resistance. (tissuelabs.com)
  • Engineering Small-Scale and Scaffold-Based Bone Organs via Endochondral Ossification Using Adult Progenitor Cells. (unibas.ch)
  • Bone tissue engineering: from bench to bedside. (mpg.de)
  • In this study, the printing capability of two different additive manufacturing (3D printing) techniques, namely PolyJet and micro-stereolithography (µSLA), are investigated regarding the fabrication of bone scaffolds. (mdpi.com)
  • Development of new methods of scaffold fabrication that closely mimic the structure and function of the extracellular matrix (ECM) is one of the main issues in tissue engineering. (springer.com)
  • The current promising fabrication technique for making scaffolds, such as computation-aided solid free-form method, can be easily applied to metals. (hindawi.com)
  • 30) C Li, L Ouyang, JPK Armstrong* & MM Stevens* "Advances in the fabrication of biomaterials for gradient tissue engineering" Trends in Biotechnology (2021). (thearmstronggroup.co.uk)
  • notes, additive manufacturing has established itself as an advantageous method for fabrication of unique and physiologically relevant structures to support tissue growth. (poly-med.com)
  • Current tissue-engineered auricular constructs implanted into immune-competent animal models have been observed to undergo inflammation, fibrosis, foreign body reaction, calcification and degradation. (biomedcentral.com)
  • Cell-scaffold constructs were induced using osteogenic induction factors for up to 8 weeks. (utwente.nl)
  • Morphology of bulk PMMA scaffolds and PMMA scaffolds with chitosan/β-TCP composite coating were studied using scanning electron microscopy (SEM). (springer.com)
  • Furthermore, chitosan/β-TCP composite coating improves the interaction between osteoblast-like cells and the polymeric scaffolds and accelerates the rate of cell proliferation. (springer.com)
  • 26 ] has shown that osteoblast survival and growth were significantly enhanced in the PLLA/HA composite scaffolds compared to the plain PLLA scaffolds. (hindawi.com)
  • Novel composite blends of poly(L-lactide-co-D,L-lactide)/tricalcium phosphate (PLDLLA/TCP) were fabricated into scaffolds by an extrusion deposition technique customised from standard rapid prototyping technology. (utwente.nl)
  • Compressive mechanical properties for the composite scaffolds were performed. (utwente.nl)
  • FDA/PI staining as well as AlamarBlue assay showed high viability of BMSCs cultured on the composite scaffolds Cell numbers, based on DNA quantitation, was observed to increase continuously up to the 8th week of study. (utwente.nl)
  • After culture with a differentiation medium for 15 days, the results indicated success in localized differentiation of stem cells in the external region of the 3D cell construct, demonstrating the possibility of producing patient-specific composite tissues through 3D bioprinting [5]. (tissuelabs.com)
  • The dimensional accuracy, permeability, and mechanical properties of complex 3D-printed scaffold structures are analyzed to compare the advantages and drawbacks associated with the two techniques. (mdpi.com)
  • Finally, as a mechanical support, a scaffold must possess adequate mechanical stability to withstand both the implantation procedure and the mechanical forces that are typically experienced at the scaffold-tissue interface and does not collapse during patient's normal activities [ 3 ]. (hindawi.com)
  • In fact, elastic modulus and other mechanical properties of these materials can be tuned by adjusting scaffold pore size, % infill, which Mohseni et al. (poly-med.com)
  • Chayanun S, Soufivand AA, Faber J, Budday S, Lohwongwatana B, Boccaccini AR, Reinforcing tissue‐engineered cartilage: Nano fibrillated cellulose enhances mechanical properties of Alginate Dialdehyde‐Gelatin (ADA‐GEL) hydrogel, Advanced Engineering Materials doi:10.1002/adem.202300641 ( 2023 ). (trr225biofab.de)
  • Fu Q, Saiz E, Rahaman MN, Tomsia AP (2011) Bioactive glass scaffolds for bone tissue engineering: state of the art and future perspectives. (springer.com)
  • We will use the lessons learnt from auricular cartilage tissue engineering to illustrate how combining additive manufacturing and regenerative medicine for tissue-engineering purposes can be used to create functional and durable tissue with potential to shift the paradigm in reconstructive surgery. (biomedcentral.com)
  • Nasal chondrocyte-based engineered autologous cartilage tissue for repair of articular cartilage defects: an observational first-in-human trial. (unibas.ch)
  • In addition to cartilage tissue engineering, the CRL applies similar approaches and technologies towards cardiovascular and cancer applications. (edu.au)
  • 2016) Self-deploying shape memory polymer scaffolds for grafting and stabilizing complex bone defects: a mouse femoral segmental defect study. (upstate.edu)
  • 32) JPK Armstrong,* E Pchelintseva, S Treumuth, C Campanella, C Meinert, TJ Klein, DW Hutmacher, BW Drinkwater & MM Stevens* "Tissue engineering cartilage with deep zone cytoarchitecture by high-resolution acoustic cell patterning" Advanced Healthcare Materials (2022). (thearmstronggroup.co.uk)
  • Dargaville, B.L. & Hutmacher, D.W. (2022) Water as the often neglected medium at the interface between materials and biology . (edu.au)
  • The association of scaffolds to repair extensive bone defects can contribute to their evolution and morphophysiological recomposition. (scielo.br)
  • We are investigating the use of novel shape-memory polymer scaffolds and electrospun shape-memory polymer sleeves as methods of rapidly stabilizing bone defects, reconstructing comminuted fractures, and delivering antimicrobial and osteoinductive agents to facilitate autologous repair long-term. (upstate.edu)
  • Regenerative Potential of Tissue-Engineered Nasal Chondrocytes in Goat Articular Cartilage Defects. (unibas.ch)
  • This method aims to regenerate rather than repair skeletal tissue defects. (openorthopaedicsjournal.com)
  • In the 21st century, advancements in tissue engineering and regenerative medicine hold the potential in developing techniques for nipple reconstruction. (wikipedia.org)
  • Melek LN. Tissue engineering in oral and maxillofacial reconstruction. (scielo.br)
  • Initial studies indicate an immense potential for cell based strategies to enhance current orthopaedic approaches in skeletal tissue reconstruction. (openorthopaedicsjournal.com)
  • Nano- to macroscale remodeling of functional tissue-engineered bone. (mpg.de)
  • Creating functional and durable tissue has the potential to shift the paradigm in reconstructive surgery by obviating the need for donor sites. (biomedcentral.com)
  • 21) L Ouyang, JPK Armstrong, M Salmeron-Sanchez & MM Stevens "Assembling living building blocks to engineer complex tissues" Advanced Functional Materials 1909009 (2020). (thearmstronggroup.co.uk)
  • Scaffolds are essential for obtaining functional tissues since they provide structural support that stimulates fixation, proliferation, and differentiation. (tissuelabs.com)
  • Tissue-engineered vascular grafts can grow and remodel and can therefore enable great advances in pediatric cardiovascular surgery. (medscape.com)
  • [ 3 ] Stenosis is the primary complication with tissue-engineered vascular grafts. (medscape.com)
  • For instance, tissue engineering and regenerative medicine can help researchers develop suitable and safe nipple implants. (wikipedia.org)
  • Development of a suitable carrier for engineering the CE to address a major clinical requirement of healthy donor tissues for transplantation. (arvojournals.org)
  • This review describes the major types and properties of bioceramics suitable for tissue engineering. (ceramic-science.com)
  • Marrow-derived stem cell motility in 3D synthetic scaffold is governed by geometry along with adhesivity and stiffness. (nature.com)
  • Mechanically, the major challenge is to achieve adequate initial strength and stiffness and to maintain them during the stage of healing or neotissues generation throughout the scaffold degradation process [ 3 , 7 , 8 ]. (hindawi.com)
  • For hard tissue applications, materials with a higher stiffness are often preferred. (poly-med.com)
  • Based on our results the PLDLLA/TCP scaffolds exhibit good potential and biocompatibility for bone tissue engineering applications. (utwente.nl)
  • Recent advances in regenerative medicine place us in a unique position to improve the quality of engineered tissue. (biomedcentral.com)
  • Due to recent advances in regenerative medicine and additive manufacturing we are entering into an age where we have the potential to replace 'like with like', by improving the quality of engineered tissue with respect to biochemical composition and functionality, as well as microstructural organization and overall shape. (biomedcentral.com)
  • We use recent advances in CRISPR genome engineering and epigenetic control of gene expression to precisely control cell activity in engineered tissues. (utah.edu)
  • Kahl M, Schneidereit D, Meinert C, Bock N, Hutmacher DW, Friedrich O, A fluorescence-based opto-mechatronic screening module (OMSM) for automated 3D cell culture workflows, Biosensors and Bioelectronics: X 14 ( 2023 ). (trr225biofab.de)
  • The first tissue-engineered, ear-shaped appendages made from bovine chondrocytes and biocompatible scaffolds by the Vacanti group were prone to deformation when xenografted onto immune-compromised mice, highlighting the lack of long-term stability [ 6 ]. (biomedcentral.com)
  • Biocompatible polymers are widely used in tissue engineering and biomedical device applications. (nature.com)
  • Andrade Mier MS, Dalton PD, Stahlhut P, Bakirci E, Blum R, Villmann C, Primary Glial Cell and Glioblastoma Morphology in Cocultures Depends on Scaffold Design and Hydrogel Composition, Adv Biol (Weinh) doi:10.1002/adbi.202300029 ( 2023 ) e2300029. (trr225biofab.de)
  • Duan B, Wang M (2010) Encapsulation and release of biomolecules from CaeP/PHBV nanocomposite microspheres and three-dimensional scaffolds fabricated by selective laser sintering. (springer.com)
  • Combining biomimetic regenerative medicine strategies will allow us to improve tissue-engineered auricular cartilage with respect to biochemical composition and functionality, as well as microstructural organization and overall shape. (biomedcentral.com)
  • In: Encyclopedia of Tissue Engineering and Regenerative Medicine, 1. (unibas.ch)
  • Three-Dimensional Bioprinting for Regenerative Dentistry and Craniofacial Tissue Engineering. (tissuelabs.com)
  • At the turn of the millennium, a new concept of regenerative bioceramics was developed and such formulations became an integrated part of the tissue engineering approach. (ceramic-science.com)
  • The bioresorbable nature of these filaments make them ideal candidates for tissue scaffolding applications and use in regenerative medicine. (poly-med.com)
  • Biologically and mechanically driven design of an RGD-mimetic macroporous foam for adipose tissue engineering applications. (unibas.ch)
  • مقدمه: امروزه به صورت گسترده‌ای از سلول‌های بنیادی مشتق از چربی (ADSCs یا Adipose tissue-derived stem cells) جهت مهندسی بافت غضروف استفاده می‌شود. (ac.ir)
  • Thus, while Dioxaprene® 100M and Caproprene™ 100M are both soft tissue-oriented, either can be selected depending on the desired degradation timeline. (poly-med.com)
  • It is also desirable for a scaffold to have high interconnectivities between pores for uniform cell seeding and distribution, and for the nutrients and metabolites exchange at the cell/scaffold construct [ 4 - 6 ]. (hindawi.com)
  • Microscopy observations showed BMSCs possessed high proliferative capabilities and demonstrated bridging across the pores of the scaffolds. (utwente.nl)
  • We use auricular cartilage as an exemplar to illustrate how the use of tissue-specific adult stem cells, assembly through additive manufacturing and improved understanding of postnatal tissue maturation will allow us to more accurately replicate native tissue anisotropy. (biomedcentral.com)
  • Despite multiple innovations over the last decades, significant limitations subsist in current clinical treatment options which is driving a strong clinical demand for clinically translatable treatment alternatives, including bone tissue engineering applications. (bvsalud.org)
  • concludes that all filaments are viable options for tissue scaffolding, with each material having unique properties to fit a range of soft and hard tissue applications. (poly-med.com)
  • In dental applications, scaffolds associated with stem cells from dental pulp or periodontal ligament are being developed to regenerate alveolar bone and periodontal tissue. (tissuelabs.com)
  • The micro-CT result from the posterolateral fusion model showed whitlockite had slightly but significantly higher percent bone volume than tricalcium phosphate, though none of the materials formed successful fusion with surrounding bone tissue. (biomedcentral.com)
  • Now bioceramic scaffolds are designed to induce bone formation and vascularization. (ceramic-science.com)
  • Namely, after the initial development of bioceramics that were just tolerated in the physiological environment, emphasis was shifted towards those able to form direct chemical bonds with the adjacent bones and tissues. (ceramic-science.com)
  • Surface tension determines tissue shape and growth kinetics. (mpg.de)
  • Due to the avascular nature of the chondral surface and the specialised rigid extracellular matrix with a low cell density this tissue rarely regenerates by itself (Mankin 1982 ). (springeropen.com)
  • Conventional recovery strategies still fail to promote widely efficient results, making 3D bioprinting an excellent candidate to mimic the complexity and multicellular interactions in these tissues [2]. (tissuelabs.com)
  • Scaffold Composition Determines the Angiogenic Outcome of Cell-Based Vascular Endothelial Growth Factor Expression by Modulating Its Microenvironmental Distribution. (unibas.ch)
  • Current research in tissue engineering is geared towards elucidating the appropriate compositional elements (biomaterials, biomolecules and cell sources) as well as methods of assembly. (biomedcentral.com)
  • Specifically, this research sought to characterize material properties and evaluate potential use in both hard and soft tissue engineering applications. (poly-med.com)
  • The combined efforts of cell biologists, material scientists, tissue engineers and reconstructive surgeons and associated converging technologies [ 1 ] in the 21st century have put us in an enviable position compared with our predecessors. (biomedcentral.com)
  • Our lab is focused on engineering cell function to better control tissue development in engineered tissues. (utah.edu)
  • These tissues are organized with complex 3D architectures and specific cell-cell interactions, which hinders the total recovery of craniofacial tissues exposed to trauma or congenital malformations. (tissuelabs.com)
  • Fetal and postnatal mouse bone tissue contains more calcium than is present in hydroxyapatite. (mpg.de)
  • Replacing the affected cornea in part (lamellar or endothelial keratoplasty) or total (penetrating keratoplasty) with a healthy donor tissue is the current accepted standard of care. (arvojournals.org)
  • However, the huge gap in the demand and supply of healthy donor tissues is a well acknowledged bottleneck for timely transplantations in many countries. (arvojournals.org)