• Immuno-electron microscopy characterization of human bone marrow stromal cells with anti-NGFR antibodies. (atsbio.com)
  • Caneva L, Soligo D, Cattoretti G, De Harven E, Deliliers GL (1995) Immuno-electron microscopy characterization of human bone marrow stromal cells with anti-NGFR antibodies. (atsbio.com)
  • These days, immunohistochemical staining is just one of several established immunostaining techniques, including enzyme-linked immunosorbent assay, flow cytometry, immuno-electron microscopy, and Western blotting. (news-medical.net)
  • Immuno-electron microscopy, also referred to as EM immunolabelling and immuno-EM, is a technique that tags antibody molecules with electron-dense substances, usually, and most effectively, being small gold particles, which are seen during the analysis as easy to spot dark dots. (news-medical.net)
  • She is also head of the Cell Microscopy Core of the UMC Utrecht, which is widely recognized as one of the best expertise centers in the world for immuno-electron microscopy. (umcutrecht.nl)
  • In the latest reports, electron microscopy, immuno-electron microscopy, and culturing from brain tissues were not performed. (alzforum.org)
  • The EM Unit provides instrumentation and services for immuno-electron microscopy, electron tomography, and specimen preparation training. (nih.gov)
  • 2011). Using dual-immuno-electron microscopy we further showed that early tau alterations initiate in Aβ accumulating synaptic terminals in the brain (Takahashi et al. (lu.se)
  • In confirmation of recent results obtained by light microscopy, somatostatin was found in all the typical D-cells containing large, weakly electron-dense secretory granules. (diabetesjournals.org)
  • Using immunogold labeling with compartmental markers and electron microscopy on neocortical biopsies from AD brain, we unequivocally identified autophagosomes and other prelysosomal autophagic vacuoles (AVs), which were morphologically and biochemically similar to AVs highly purified from mouse liver. (elsevierpure.com)
  • This study aimed to apply electron and immunoelectron microscopy (EM and IEM) approaches for ultrastructural study of cellular processes and to quantify distributions of relevant proteins across subcellular compartments under chronic diseases such as HCV and DFUs or after HPP therapy of DFUs. (uclv.cu)
  • Immune electron microscopy (more often called immunoelectron microscopy) is the equivalent of immunofluorescence, but it uses electron microscopy rather than light microscopy. (wikipedia.org)
  • Scanning electron microscopy is a viable option if the antigen is on the surface of the cell, but transmission electron microscopy may be needed to see the label if the antigen is within the cell. (wikipedia.org)
  • Transmission electron microscopy then detects the antibody and, therefore, the protein. (wikipedia.org)
  • While using immune electron microscopy, the specimen can either be in thin sections so the electrons can penetrate it or negatively stained. (wikipedia.org)
  • When used in immune electron microscopy, negative staining implants a small particle into the specimen, better resolving structures within it. (wikipedia.org)
  • Results from immune electron microscopy are typically quantified visually. (wikipedia.org)
  • During successful experiments, immune electron microscopy can accurately locate proteins and strengthen comprehension of the relationship between structure and function. (wikipedia.org)
  • This invention led to the scanning electron microscope and transmission electron microscope, which later contributed to immunoelectron microscopy. (wikipedia.org)
  • Transmission electron microscopy successfully provides general information about structure but struggles to differentiate more detailed parts of a virus or cell. (wikipedia.org)
  • Diagnostic electron microscopy has two advantages over ance of vesicular lesions or respiratory illness in farm animals enzyme-linked immunosorbent assay and nucleic acid amplifi- may be evidence of an emerging disease, a possible zoonosis, cation tests. (cdc.gov)
  • While recent studies suggest that health- the undirected, "open view" of electron microscopy allows rapid care systems are ill prepared to treat victims and contain the morphologic identification and differential diagnosis of different spread of an infectious agent (5), the performance of physi- agents contained in the specimen. (cdc.gov)
  • Applications of diagnostic electron microscopy in clinically or outbreak-associated agents as diverse as Nipah virus (6) and epidemiologically critical situations as well as in bioterrorist gastroenteric agents (7) indicate that identification of an out- events are discussed. (cdc.gov)
  • Electron microscopy can be applied to break and its associated agent may be done rapidly and suc- many body samples and can also hasten routine cell culture cessfully. (cdc.gov)
  • Electron microscopic diagnosis is uniquely suited for rapid microscopy fully, it should be quality controlled, applied as a identification of infectious agents. (cdc.gov)
  • Thus, late 1950s (12) and the wider availability of electron micro- the most potent defenses remain rapid identification of the scopes, electron microscopy (as a catchall method) became event and agent, treatment of the victims, and containment of essential in characterizing many new isolates detected in diag- infection. (cdc.gov)
  • Visual and photographic microscopy in which electron beams with wavelengths thousands of times shorter than visible light are used in place of light, thereby allowing much greater magnification. (genomicglossaries.com)
  • In high-resolution electron microscopy one can begin to do `` crystallography without crystals'', averaging thousands of images of single molecules or other assemblies to reveal near atomic level structure. (genomicglossaries.com)
  • Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana- Champaign Molecular Biomedicine in the Era of Teraflop Computing - DDDAS.org Narrower term: transmission electron microscopy (TEM). (genomicglossaries.com)
  • Microscopy in which the samples are first stained immunocytochemically and then examined using an electron microscope. (genomicglossaries.com)
  • 2009-2014: WP4: Coordinator for Correlative Light Electron Microscopy. (umcutrecht.nl)
  • By electron microscopy, most DβH terminals did not make synapses, but when they did, they formed both asymmetric and symmetric synapses. (frontiersin.org)
  • By electron microscopy, βAR 248 was present in neuronal cell bodies, dendritic shafts and spines, and some axon terminals and astrocytes. (frontiersin.org)
  • Conventional labeling approach in transmission and scanning electron microscopy utilizes secondary immunogold reagents based on particles that can be observed without enhancement. (emsdiasum.com)
  • Semithin (90 nm) cryosections were cut at -110C with an UltraCut UCT/FCS cryomicrotome (Leica), using a diamond knife (Diatome) and transferred to a Formvar-coated, carbon-coated, glow-discharged 100-mesh copper-rhodium electron microscopy grid. (cellimagelibrary.org)
  • BACKGROUND: Accurate diagnosis of epidermolysis bullosa (EB) has significant implications for prognosis, management, and genetic counseling.OBJECTIVE: To describe diagnostic testing patterns and assess diagnostic concordance of transmission electron microscopy (TEM), immunofluorescence mapping (IFM), and genetic analysis for EB.METHODS: A retrospective cohort of patients enrolled in the Epidermolysis Bullosa Clinical Characterization and Outcomes Database from January 1, 2004 to July 8, 2019 was included. (stanfordchildrens.org)
  • In the RRBP1 -knockdown Calu-6 cells, a human renin-producing cell line, transmission electron and confocal microscopy revealed that renin was primarily retained in the endoplasmic reticulum and was unable to efficiently target the Golgi apparatus for secretion. (biomedcentral.com)
  • In contrast to the classical binary fission used by most bacteria, electron microscopy reveals that ROLP exploits an asymmetrical budding strategy to replicate in plant hosts and insect vectors. (biomedcentral.com)
  • This study elucidated the etiology of C3 glomerulonephritis (C3GN) and non-C3GN with primary membranoproliferative glomerulonephritis (MPGN) using transmission electron microscopy (TEM) and periodic acid-methenamine silver stain (PAM-EM). (bvsalud.org)
  • Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. (bvsalud.org)
  • In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. (bvsalud.org)
  • In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen. (bvsalud.org)
  • In order to correlate the different cell types of the human endocrine pancreas to a specific secretion product, an immunoelectron microscopic localization of the hormones whose production had been attributed to pancreatic islets was conducted. (diabetesjournals.org)
  • In the present study, we investigated the localization of PRKRA in the mammalian testis by immunofluorescence and immunoelectron microscopic techniques. (hindawi.com)
  • Using double-labelling immunoelectron microscopic techniques, co-clustering between GABA B1 and GIRK2 was detected in dendritic spines, whereas they were mainly segregated in the dendritic shafts. (springer.com)
  • B-D , Images from triple-labeling experiments in sections from GFP-GAD67 mice showing the colocalization of α2 subunit-positive clusters (red) with gephyrin (blue) inside eGFP-positive GC dendrites (green) in the EPL, as visualized by confocal microscopy. (jneurosci.org)
  • Confocal microscopy revealed that both excitatory and inhibitory neurons express βAR248. (frontiersin.org)
  • Immunoelectron microscopy identifies and localizes a molecule of interest, specifically a protein of interest, by attaching it to a particular antibody. (wikipedia.org)
  • In this study, a specific antibody against the immunodominant membrane protein (Imp) of rice orange leaf phytoplasma (ROLP) was generated and subjected to the frozen section immune gold labeling assay, immune gold labeling microscopy, and immune fluorescence labeling assay to investigate how ROLP enters, propagates, and spreads in rice and its leafhopper vector Recilia dorsalis at the ultrastructural level. (biomedcentral.com)
  • Previously, we investigated the localization of several nuage-resident proteins in the nuage structures of spermatogenic cells by immunoelectron microscopy (IEM) [ 22 - 26 ]. (hindawi.com)
  • The development of new probe technologies, such as quantum dots and high-resolution laser fluorescence microscopy, allow real- time observations of molecular interactions and trafficking within living cells. (genomicglossaries.com)
  • Virus was detected in serum by counter-current immunoelectrophoresis (CIE) and immunoelectron microscopy (IEM). (bmj.com)
  • The SPM unit specializes in complex microscopy and spectroscopy techniques, mathematical models, and image analysis of cells, tissues, and molecular complexes. (nih.gov)
  • Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. (genomicglossaries.com)
  • By light microscopy, βARs were present in both neurons and astrocytes. (frontiersin.org)
  • The benefit of immunoelectron microscopy is that it allows for the recognition of particles no matter the context. (wikipedia.org)
  • To understand better the cellular mechanisms of NE's contributions to fear learning, we examined the anatomical organization of NE terminals and βARs in the LA. In this study, we employed immunoelectron microscopy to determine whether terminals immunoreactive for dopamine beta-hydroxylase (DβH), the synthetic enzyme for NE, form synaptic junctions in the LA and if so, examine these synapses and identify the post-synaptic targets on NE terminals. (frontiersin.org)
  • Additionally, the lungs were also assessed for desmosine content and for the determination of elastase levels in the pulmonary interstitium by means of immunoelectron microscopy. (biomedcentral.com)
  • With immunoelectron microscopy in pemphigus erythematosus, IgG and C3 deposits are localized to the epidermal cell membranes and the upper dermis. (medscape.com)
  • Immunoelectron microscopy came about when two independent groups in the 1940s combined the tobacco mosaic virus and its antiserum. (wikipedia.org)
  • A type of scanning probe microscopy in which a probe systematically rides across the surface of a sample being scanned in a raster pattern. (genomicglossaries.com)
  • B-D , Images from triple-labeling experiments in sections from GFP-GAD67 mice showing the colocalization of α2 subunit-positive clusters (red) with gephyrin (blue) inside eGFP-positive GC dendrites (green) in the EPL, as visualized by confocal microscopy. (jneurosci.org)
  • The morphology of the cytoskeleton was detected by laser confocal microscopy, and RhoA activities were determined by fluorescence resonance energy transfer (FRET) microscopy. (frontiersin.org)
  • 1 in the spermatogenic cells of rat testis were studied by immunofluorescence and immunoelectron microscopy. (scirp.org)
  • Using ultrastructure expansion microscopy, we show that LRRCC1, the ortholog of a protein originally characterized in flagellate green algae, associates preferentially to two consecutive triplets in the distal lumen of human centrioles. (elifesciences.org)
  • Immunoelectron microscopy is used extensively in diagnostic virology as part of very sensitive immunoassays. (bvsalud.org)
  • Immunoelectron microscopy revealed that the major subcellular localization sites for NANOS1 were the chromatoid body (CB) and satellite body (SB), and the minor sites were the rest of the nuage compartments, including the irregularly-shaped perinuclear granules (ISPG), and intermitochondrial cement (IMC). (scirp.org)
  • Analysis of JAM-C subcellular expression by immunoelectron microscopy indicated that in I/R-injured tissues, EC JAM-C was redistributed from cytoplasmic vesicles and EC junctional sites to nonjunctional plasma membranes, a response that may account for the role of JAM-C in both leukocyte adhesion and transmigration under conditions of I/R injury. (unige.ch)
  • Elementary bodies of C.pneumoniae with typical ultrastructural characteristics were also identified in serum sediments using immunoelectron microscopy. (medsci.org)
  • Immunoelectron microscopy identifies and localizes a molecule of interest, specifically a protein of interest, by attaching it to a particular antibody. (wikipedia.org)
  • In addition, immunoelectron microscopy has been used to provide further information on protein localization. (medscape.com)
  • In this study, we use immunoelectron microscopy and confocal imaging to provide quantitative analysis of LAMP1 distribution in various autophagic and endolysosomal organelles in neurons. (nih.gov)
  • Immunoelectron microscopy of Flublok (Sanofi Pasteur), a commercially available influenza vaccine from 2017 to 2018 containing hemagglutinin from H1N1, H3N2, and influenza B viruses. (nih.gov)