AntigenConjugatesEnzymeDetectionPhenolElectronMonoclonalOxidationAntibodyGoatELISAEnzymesAcidicGlycoproteinStabilitySubstratesExperimentalSignalActivityStudiesApplicationsMethodHighEnzyme activity of horseradish peroxidaseAntibodyOxidation of luminolHorse Radish PeroxidaseSubstrateBiochemistryHydrogen peroxideAlkaline PhosphataseVitroBoundCatalyticCatalyzeMeSHPolymerizationMajorORDER
Antigen3
- Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. (jacksonimmuno.com)
- In order to early diagnose breast cancer with the rapid quantification of carcinoembryonic antigen (CEA) in human serum, highly sensitive DNA aptasensor with 1,1-oxalyldiimidazole chemiluminescence (ODI-CL) detection was developed using the combination of CEA aptamer, horseradish peroxidase (HRP)-mimic hemin, and graphene sheet in a polystyrene well. (techconnect.org)
- An enzyme isolated from horseradish which is able to act as an antigen. (bvsalud.org)
Conjugates3
- The utility of enhanced chemiluminescent assays for horseradish peroxidase conjugates was demonstrated in a range of immunoassays, both immunoextraction and competitive, in conjunction with a variety of solid supports such as plastic beads, tubes and microtitre plates. (bham.ac.uk)
- Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. (jacksonimmuno.com)
- Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. (jacksonimmuno.com)
Enzyme8
- The enzyme horseradish peroxidase (HRP), found in the roots of horseradish, is used extensively in biochemistry applications. (wikipedia.org)
- Peroxidase, a heme-containing oxidoreductase, is a commercially important enzyme which catalyses the reductive cleavage of hydrogen peroxide by an electron donor. (wikipedia.org)
- Moreover, "In recent years the technique of marking neurons with the enzyme horseradish peroxidase has become a major tool. (wikipedia.org)
- This research investigated the applicability of a luminol-based chemiluminescence assay to monitor transport of horseradish peroxidase (HRP) enzyme in saturated porous media. (k-state.edu)
- Language": "en", "Country": "XG", "Code": "CB - Order Information" }, { "Name": "Applications", "Value": "Peroxidase (POD), Grade I is a marker enzyme enabling peroxidation of reduced dyes in the indicator reaction producing a color, fluorimetric or luminescent derivative of the labeled molecule for further detection and quantification. (roche.com)
- For the enzyme tube, instead of distilled water add the pH solution (3) and 1.5 mL of peroxidase which equals a total volume of 7.5 mL. (ipl.org)
- None (Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase. (jacksonimmuno.com)
- Enzyme immobilized onto the macroporous copolymer with the pore size diameter of 234 nm showed a substantial degree of phenol removal achieved by immobilized peroxidase. (ac.rs)
Detection2
- Some cell-permeable fluorogenic probes developed are useful for the detection of ROS while in conjunction with horseradish peroxidase (HRP). (tmu.edu.tw)
- 1) Horseradish (Armoracia rusticana) peroxidase is used in detection systems with different substrates. (bspp.org.uk)
Phenol1
- Certain 6-hydroxybenzothiazole and phenol derivatives enhance light emission from the horseradish peroxidase catalysed oxidation of cyclic diacyl hydrazides such as luminol. (bham.ac.uk)
Electron3
- The M-cell specificity of the lectin binding was further demonstrated by localization of horseradish peroxidase (HRP)-conjugated lectins under the transmission electron microscope (TEM). (nih.gov)
- In this work, a novel sensing scaffold, consisting Au nanoparticle (GNP)-dotted TiO 2 nanotubes (TNTs) as the rigid material and the hydrophobic ionic liquid (HIL), 1-decyl-3-methylimidazolium tetrafluoroborate, as the entrapping agent, was applied to facilitate the electron transfer of horseradish peroxidase (HRP) on a glassy carbon electrode. (edu.au)
- We employed experimental degeneration, tract-tracing with wheatgerm agglutinin conjugated with horseradish peroxidase (WGA-HRP) and electron microscopy to explore the postganglionic sympathetic fibers in the hypoglossal nerve of hamsters. (tmu.edu.tw)
Monoclonal1
- A monoclonal mouse anti-peroxidase antibody is a very useful and reliable reagent for the localization of a number of mouse primary antibodies by peroxidase anti-peroxidase (PAP) immunocytochemistry. (medimabs.com)
Oxidation1
- Horseradish peroxidase catalyses the oxidation of luminol to 3-aminophthalate via several intermediates. (wikipedia.org)
Antibody4
- A sensitive antibody against horseradish peroxidase (HRP). (medimabs.com)
- Peroxidase-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity. (nordicmubio.com)
- For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells. (jacksonimmuno.com)
- Bound antibody was detected using avidin-biotin-peroxidase (Vector Labs) and diaminobenzidine (DAB). (cellimagelibrary.org)
Goat2
- Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat antiSerum to C3c fragment of Monkey complement factor C3. (nordicmubio.com)
- Peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). (nordicmubio.com)
ELISA1
- Horseradish peroxidase is also commonly used in techniques such as ELISA and Immunohistochemistry due to its monomeric nature and the ease with which it produces coloured products. (wikipedia.org)
Enzymes1
- In addition to biomedical applications, horseradish peroxidase is one of the enzymes with important environmental applications. (wikipedia.org)
Acidic2
- Usage of more abundant, basic isoforms of peroxidases in diagnostic kits and/or in immunochemistry has led to under exploitation and disregard of horseradish peroxidase (HRP) acidic isoforms. (ac.rs)
- Therefore, acidic horseradish peroxidase (HRP-A) isoenzymewas used for the preparation of a biocatalyst with improved ability in dye decolorization. (ac.rs)
Glycoprotein2
- citation needed] Horseradish peroxidase is a 44,173.9-dalton glycoprotein with 6 lysine residues which can be conjugated to a labeled molecule. (wikipedia.org)
- Horseradish peroxidase (HRP) is a 40kDa glycoprotein with four lysine residues. (medimabs.com)
Stability2
- High concentrations of phosphate severely decrease stability of horseradish peroxidase. (wikipedia.org)
- Stability studies showed an improved operational stability of immobilized peroxidase at 65 °C and in an organic solvent. (ac.rs)
Substrates1
- Numerous substrates for horseradish peroxidase have been described and commercialized to exploit the desirable features of HRP. (wikipedia.org)
Experimental1
- Su antigenicidad ha permitido su empleo como antígeno y marcador combinado en inmunología experimental. (bvsalud.org)
Signal1
- Horseradish peroxidase (HRP) was used as a signal reporter for electrochemical readout. (cdc.gov)
Activity2
- In particular, in highly viscous concentrated solutions, water activity, which is a thermodynamical variable extensively used to describe the chemical potential of water in binary and complex systems, could play a significant role [5].The aim of this study was to investigate and compare the effect of viscosity and water activity (aw) on the activity of horseradish peroxidase (HRP) and bovine lactoperoxidase (LPO) in buffered solutions. (unite.it)
- Herein, a new approach for intracellular sensing of ROS by using horseradish peroxidase-encapsulated hollow silica nanospheres (designated HRP@HSNs), with satisfactory catalytic activity, cell membrane permeability, and biocompatibility, was prepared via a microemulsion method.These HRP@HSNs, combined with selective probes or targeting ligands, could be foreseen as ROS-detecting tools in specific organelles or cell types. (tmu.edu.tw)
Studies1
- Studies on horseradish peroxidase C and other haem peroxidases have been carried out on selected mutants in the distal haem cavity providing insight into the functional importance of the distal residues. (figshare.com)
Applications3
- Horseradish peroxidase is ideal in many respects for these applications because it is smaller, more stable, and less expensive than other popular alternatives such as alkaline phosphatase. (wikipedia.org)
- Peroxidases (EC 1.11.1.7) have enormous biotechnological applications. (ac.rs)
- Micic AU - Vujčić, Zoran AU - Vujčić, Miroslava PY - 2017 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2435 AB - Peroxidases (EC 1.11.1.7) have enormous biotechnological applications. (ac.rs)
Method1
- The glutaraldehyde and periodate method were employed to immobilize horseradish peroxidase (HRP) onto these carriers. (ac.rs)
High2
- The temperature in our experiment was not very high which didn't result in denaturation of peroxidase. (ipl.org)
- The Catalytic Pathway of Horseradish Peroxidase at High Resolution Nature V. 417 463 2002 . (atomistry.com)
Enzyme activity of horseradish peroxidase2
- None (Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase. (jacksonimmuno.com)
- Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. (antikoerper-online.de)
Antibody3
- For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells. (jacksonimmuno.com)
- Based on immunoelectrophoresis and/or ELISA, the antibody reacts with peroxidase from horseradish roots. (licor.com)
- Bound antibody was detected using avidin-biotin-peroxidase (Vector Labs) and diaminobenzidine (DAB). (cellimagelibrary.org)
Oxidation of luminol2
- Horseradish peroxidase catalyses the oxidation of luminol to 3-aminophthalate via several intermediates. (wikipedia.org)
- In vitro , the chemiluminescence-producing oxidation of luminol by the enzyme horseradish peroxidase yields three metabolites, one of which is 3-aminophthalic acid. (nih.gov)
Horse Radish Peroxidase1
- Enzymatic Iodination of Protein: Kinetics of Iodine Formation and Protein Iodination Catalyzed by Horse-Radish Peroxidase. (nih.gov)
Substrate1
- Structural interactions between horseradish peroxidase C and the substrate benzhydroxamic acid determined by X-ray crystallography. (nih.gov)
Biochemistry1
- The enzyme horseradish peroxidase (HRP), found in the roots of horseradish, is used extensively in biochemistry applications. (wikipedia.org)
Hydrogen peroxide2
- Peroxidase, a heme-containing oxidoreductase, is a commercially important enzyme which catalyses the reductive cleavage of hydrogen peroxide by an electron donor. (wikipedia.org)
- The enzyme used was horseradish peroxidase which helps catalyze hydrogen peroxide. (ipl.org)
Alkaline Phosphatase1
- Horseradish peroxidase is ideal in many respects for these applications because it is smaller, more stable, and less expensive than other popular alternatives such as alkaline phosphatase. (wikipedia.org)
Vitro2
- Peritoneal mesothelium was exposed for 2-60 min to solutions of horseradish peroxidase by incubation in vitro, or after intraperitoneal injection in vivo. (rupress.org)
- Benzo[a]pyrene (BP) was bound to DNA by horseradish peroxidase, rat liver microsomes, and rat liver nuclei in vitro and in mouse skin in vivo. (nih.gov)
Bound1
- Here we describe an effective strategy to obtain crystal structures for high-valency redox intermediates and present a three-dimensional movie of the X-ray-driven catalytic reduction of a bound dioxygen species in horseradish peroxidase (HRP). (nih.gov)
Catalytic1
- Catalytic polymerization of bisphenol A using a horseradish peroxidase immobilized microporous membrane reactor. (nih.gov)
Catalyze1
- The enzyme of turnip peroxidase is added in the equation to catalyze the oxidation. (ipl.org)
MeSH1
- Horseradish Peroxidase" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
Polymerization1
- Herein, the first enzymatic polymerization of horseradish peroxidase (HRP) is reported. (elsevierpure.com)
Major2
- Moreover, "In recent years the technique of marking neurons with the enzyme horseradish peroxidase has become a major tool. (wikipedia.org)
- This graph shows the total number of publications written about "Horseradish Peroxidase" by people in this website by year, and whether "Horseradish Peroxidase" was a major or minor topic of these publications. (umassmed.edu)
ORDER1
- Bulk Order Inquiry for Peroxidase Stabilizing Buffer ------- (please add any order requirements, including desired quantity, timing, etc. (immunochemistry.com)