Hepg2 hepatocyte nuclear factor 1. Medical search. Frequent questions

Chromatin immunoprecipitation experiments demonstrated that HNF4α and AP-1, but not C/EBPβ, reciprocally bound to this site prior to and after treating HepG2 cells with IsoNAM. (elsevierpure.com)

Metastasis is a major cause of death in patients with hepatocellular carcinoma (HCC), although survival has improved due to advances in surgical techniques [ 1 ]. (oncotarget.com)
The gene expression profile of HepG2 human hepatocellular carcinoma cells transfected with the apoO silencing vector was investigated using a whole-genome oligonucleotide microarray. (biomedcentral.com)
Hepatocellular carcinoma (HCC) has become the leading cause of deaths that are caused by all cancers worldwide [1] and the incidence and mortality of HCC are increasing at a faster pace than other cancers, which trend will continue through at least 2030 in United States [2]. (researchsquare.com)

Autophagy also regulates cell proliferation, differentiation, survival, and apoptosis, highlighting its role in maintaining cellular homeostasis [ 1 ]. (hindawi.com)
The NFAT2 overexpression suppressed the cell viability, invasion and migration capabilities, and promoted apoptosis of HepG2 cells. (researchsquare.com)
In this study, we constructed NFAT2 overexpressed cells (HepG2/NFAT2) based on HepG2 and investigated the effect of NFAT2 overexpression on HepG2 cells' viability, apoptosis, proliferation, invasion and migration. (researchsquare.com)
NQ induced apoptosis in HepG2 cells by activating p53-ROS crosstalk and induces epigenetic modifications leading to inhibited proliferation and cell cycle arrest. (molcells.org)

14] "The expression of the solute carriers NTCP and OCT-1 is regulated by cholesterol in HepG2 cells. (tcdb.org)
HepG2 cells were incubated with troglitazone, its metabolites M-1 (sulfate), M-2 (gulucronide), M-3 (quinone), and other thiazolidinediones (pioglitazone and rosiglitazone). (nih.gov)
To further study the role of tTG in liver disease, we initiated investigations into the effect of a proinflammatory mediator, tumor necrosis factor (TNF)-alpha, on tTG activity in cultured liver cells. (nih.gov)
Treatment of human Hep G2 cells with 1 ng/ml TNF-alpha increased [14C]putrescine cross-linking to cellular proteins. (nih.gov)
Gel shift experiments using nuclear extracts from TNF-alpha-treated cells and oligonucleotides containing the tTG nuclear factor (NF)-kappa B motif revealed increased binding, concordant with mRNA data. (nih.gov)
HepG2 cells were used to further explore the underlying mechanisms in vitro . (karger.com)
Oleic-acid (OA) added to rat hepatocytes or HepG2 cells in culture produced cellular-phenotypic LD modifications: increases in TAG, CE, C, and PL content and in cLD and nLD numbers and sizes. (unlp.edu.ar)
In HepG2 cells, exendin-4-reversed lipid deposition induced by palmitate was hampered when SIRT1 was silenced by SIRT1 RNA interference. (diabetesjournals.org)
But modest Promotoraktivit t In cellular Ren reporter assays based on typical erh Ht, probably because the CAR accumulates in the nucleus of immortalized cells, w While they Haupt Normally in the cytoplasm of primary Ren hepatocytes and liver. (c-kitinhibitors.com)
Mediate dexamethasone, a glucocorticoid Mimic the active CYP2C promoters in HepG2 cells on the glucocorticoid receptor Of. (c-kitinhibitors.com)
It can also be provided through the induction of CYP2C8 Lithochols Acid in HepG2 cells. (c-kitinhibitors.com)
Immunoprecipitation assays using PXR-overexpressing HepG2 (ShP51) cells revealed that PXR interacts with not only DAZAP1 but also NEAT1_2, a long noncoding RNA included in the paraspeckle, and that the interaction between PXR and NEAT1_2 was disassociated by rifampicin. (aspetjournals.org)
Induction of CYP3A4 by rifampicin was significantly enhanced by the knockdown of NEAT1_2 or DAZAP1 in ShP51 cells and their parental HepG2 cells. (aspetjournals.org)
Free circulating insulin activates insulin receptors on the surface of target cells eliciting a signaling cascade initiated by the activation of insulin receptor substrates (IRS 1-4) followed by phosphorylation of phosphoinositide 3-kinase (PI3K), which manages metabolic response including PDK1 and Akt stimulation by phosphorylation. (encyclopedia.pub)
To further determine whether BCR ABL phosphorylates Raf Inhibitors hTERT, we treated K562 cells with 1 M Gleevec, and evaluated the phosphorylation status of hTERT. (rafinhibitors.com)
Gleevec inhibits hTERT nucleoli translocation in K562 BCR ABL positive cells It is known that phosphorylation of hTERT is important for its nuclear translocation. (rafinhibitors.com)
HepG2 cells were treated either with oleic acid or tumor necrosis factor-α for 24 h. mRNA and protein expression of apoO were assessed by quantitative real-time PCR (qRT-PCR) and Western blot respectively. (biomedcentral.com)
ApoO expression in HepG2 cells was dramatically affected by lipid and inflammatory stimuli. (biomedcentral.com)
A total of 282 differentially expressed genes in apoO-silenced HepG2 cells were identified by microarray analysis. (biomedcentral.com)
It is likely that apoO participates in fatty acid metabolism and the inflammatory response in HepG2 cells, and UCP2 may act as a mediator between lipid metabolism and inflammation in apoO-silenced HepG2 cells. (biomedcentral.com)
A microarray assay was used to identify genes that are differentially expressed in apoO-silenced HepG2 cells. (biomedcentral.com)
We determined if a lipid stimulus would influence the expression of apoO in HepG2 cells. (biomedcentral.com)
Figure 1 shows apoO mRNA and protein levels in cells treated with oleic acid (OA) compared with the levels in control cells. (biomedcentral.com)
ApoO mRNA and protein changes in HepG2 cells pretreated with 1 mmol/L OA for 24 h. (biomedcentral.com)
To explore the effect of inflammatory stimulus on the expression of apoO, we detected apoO mRNA and protein expression changes in HepG2 cells pretreated for 24 h with 100 ng/mL tumor necrosis factor-α (TNF-α). (biomedcentral.com)
Levels of ApoO mRNA and protein in HepG2 cells pretreated with 100 ng/mL TNF-α for 24 h. (biomedcentral.com)
Lane 5: ZR75-1 cells. (novusbio.com)
In vitro, conditioned medium from exo-adipocytes promoted HepG2 cell migration and increased tube formation of human umbilical vein endothelial cells (HUVECs). (biomedcentral.com)
Nuclear factor of activated T cells 2 (NFAT2) has been reported to regulate the development and malignancy of few tumors. (researchsquare.com)
Firstly, the pcDNA3.1-NFAT2 plasmid was transfected into HepG2 cells to construct NFAT2 overexpressed HepG2 cells. (researchsquare.com)
The sensitivity and Ca 2+ mobilization of HepG2 cells was also inhibited by NFAT2 overexpression. (researchsquare.com)
The current study firstly suggested that NFAT2 suppressed the aggression and malignancy of HepG2 cells through inducing the expression of Egr2. (researchsquare.com)
Nuclear factor of activated T cells 2 (NFAT2, also known as NFATc and NFATc1) is an important member of NFAT family which plays a vital role in T cell activation and differentiation [4]. (researchsquare.com)
Furthermore, NFAT2 participates in the early growth response factor 2 (Egr2)-regulated anergic phenotype which impairs the migration and invasion of leukaemia cells [16]. (researchsquare.com)
Another statement showed the preadipocyte- and endothelial cell-derived stromal-derived element-1 (CXCL12), mediated early infiltration of CD4+ T lymphocytes in obesity, which preceded the increase of macrophages in adipose cells of mice on HFD (101). (exposed-skin-care.net)
We loaded gold-quercetin into poly(DL-lactide-co-glycolide) nanoparticles (NQ) and tested the biological activity of NQ on HepG2 hepatocarcinoma cells to acquire the sustained release property. (molcells.org)

The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. (cancerindex.org)
Studies on rodent UGT genes have demonstrated that the transcription factors hepatocyte nuclear factor 1 (HNF1) and CAAT-enhancer binding protein are important positive regulators of UGT expression in the liver (Hansen et al. (aspetjournals.org)
nLD could be involved in nuclear-lipid homeostasis serving as an endonuclear buffering system that would provide or incorporate lipids and proteins involved in signalling pathways as transcription factors and as enzymes of lipid metabolism and nuclear processes. (unlp.edu.ar)
Nuclear receptor-mediated transcriptional activation of genes by drugs CYP2C in Liver transcriptional activation of most P450 genes is mediated by nuclear receptors sensitive drugs, the transcription factors of detecting foreign matter are Rpern. (c-kitinhibitors.com)
Insulin signaling affects multiple downstream pathways including mitogen-activated protein kinase (MAPK), which controls growth, sterol regulatory element-binding protein 1 (SREBP-1), which stimulates the synthesis of lipid and cholesterol as well as the family of Forkhead (FOXO) transcription factors regulating metabolism and autophagy [ 9 ] [ 10 ] . (encyclopedia.pub)
Once NFAT2 is activated, it exposes nuclear localization sequences, promoting its cytoplasm-to-nuclear translocation and then NFAT2 forms heterodimers with other transcription factors to exert its transcriptional functions [6]. (researchsquare.com)

GLP-1 and incretin mimetics, such as exenatide, have been shown to attenuate hepatocyte steatosis in vivo and in vitro, but the specific underlying mechanism is unclear. (diabetesjournals.org)
Here, we speculate that SIRT1 might mediate the effect of the GLP-1 receptor agonist exenatide (exendin-4) on ameliorating hepatic steatosis. (diabetesjournals.org)
Our data demonstrate that SIRT1 mediates the effect of exenatide on ameliorating hepatic steatosis, suggesting the GLP-1 receptor agonist could serve as a potential drug for nonalcoholic fatty liver disease (NAFLD), especially in type 2 diabetes combined with NAFLD, and SIRT1 could be a therapeutic target of NAFLD. (diabetesjournals.org)

These results suggest that troglitazone induces apoptotic hepatocyte death which it may be one of the factors of liver injury in humans. (nih.gov)

Neutral lipids - involved in many cellular processes - are stored as lipid droplets (LD), those mainly cytosolic (cLD) along with a small nuclear population (nLD). (unlp.edu.ar)

Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2) . (rndsystems.com)
Sterol regulatory element-binding proteins (SREBPs) compose a family of transcriptional factors that regulate the expression of various genes required for the synthesis of phospholipids, fatty acids, and cholesterol. (novusbio.com)
Additionally, a total of 1428 proteins were identified in HepG2 exosomes by mass spectrometry. (biomedcentral.com)

Xu N, Zhang XY, Dong X, Ekstrom U, Ye Q, Nilsson-Ehle profile: complications of using address, neck vessel blood, and information on the blood of apolipoprotein M in HepG2 companies. (ra-berg.de)

After the activation by exposure to xenobiotic nuclear receptors bind to influence response elements as monomers or homo or hetero dimers recruit school coactivators chromatin structure and the transcription of target genes. (c-kitinhibitors.com)
Several nuclear receptors have been identified xenobiotic-induced activation of gene transcription human CYP2C are involved. (c-kitinhibitors.com)
Members of the family of nuclear receptors, the three peroxisome proliferator-activated receptors (PPARs), PPARα, PPARβ/δ, and PPARγ take part in the modulation of these pathways. (encyclopedia.pub)
Analytes include soluble growth and differentiation factors, extracellular matrix components, proteases, membrane-bound receptors, and intracellular signaling molecules. (rndsystems.com)

An increase in tTG mRNA content was observed 1 h after addition of TNF-alpha, and levels of tTG mRNA remained elevated after 24 h. (nih.gov)
Iso-NAM decreased the level of mRNA for PEPCK-C but had no effect on mRNA for glucose-6-phosphatase in AML12 mouse hepatocytes. (elsevierpure.com)
The induction of mRNA and protein of CYP2C19 shows a large interindividual variability en t In the human liver. (c-kitinhibitors.com)

2016. Design and synthesis of potent in vitro and in vivo anticancer agents based on 1-(3′,4′,5′-Trimethoxyphenyl)- 2-Aryl-1H-Imidazole . (lsrnw.ac.uk)

1 , 2 In recent years, mammalian cell-derived exosomes (a type of extracellular vesicle) have been widely used in the field of biomedicine, including diagnosis, regulation of disease progression and drug delivery. (dovepress.com)
Exosomes were isolated from HCC cell line HepG2 and added to adipocytes. (biomedcentral.com)
Our results demonstrated that HCC cell HepG2-derived exosomes could be actively internalized by adipocytes and caused significant transcriptomic alterations and in particular induced an inflammatory phenotype in adipocytes. (biomedcentral.com)
Mechanistically, we found HepG2 exosomes activated several phopho-kinases and NF-κB signaling pathway in exo-adipocytes. (biomedcentral.com)

CAR constitutively active ligand and without many xenobiotics primarily act by inducing its nuclear translocation t would, as a ligand. (c-kitinhibitors.com)

ObjectiveThe aim of this study was to document the association between pancreatic agenesis or hypoplasia and multicystic renal dysplasia related to transcription factor 2 (TCF2) or hepatocyte nuclear factor 1 beta mutations. (cyp17-signal.com)

Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function. (cancerindex.org)
The SIRT1 activators isonicotinamide (IsoNAM), resveratrol, fisetin, and butein repressed transcription of the gene for the cytosolic form of phosphoenolpyruvate carboxykinase (GTP) (PEPCK-C). An evolutionarily conserved binding site for hepatic nuclear factor (HNF) 4α (-272/ -252) was identified, which was required for transcriptional repression of the PEPCK-C gene promoter caused by these compounds. (elsevierpure.com)
The nuclear receptor CAR is responsible for transcriptional activation of CYP2C9, CYP2C8 and CYP2C19. (c-kitinhibitors.com)

1] "Cloning and characterization of two human polyspecific organic cation transporters. (tcdb.org)
3] "Molecular cloning, functional characterization and genomic organization of four alternatively spliced isoforms of the human organic cation transporter 1 (hOCT1/SLC22A1). (tcdb.org)
12] "The human organic cation transporter-1 gene is transactivated by hepatocyte nuclear factor-4alpha. (tcdb.org)
18] "Seven novel single nucleotide polymorphisms in the human SLC22A1 gene encoding organic cation transporter 1 (OCT1). (tcdb.org)
The best known of the human env-derived genes is syncytin-1, or ERVWE1, which originated from a human endogenous retrovirus (HERV) of the HERV-W family inserted on chromosome 7q21 in human-pre-hominoids 40-45 million years ago [ 11 , 10 ]. (ecancer.org)
Solated prim Ren human hepatocytes, which are listed in Table 1. (c-kitinhibitors.com)
The vitamin D receptor has been reported to produce a modest 2 times the induction of CYP2C9 in prime Ren human hepatocytes by 1,25 dihydroxyvitamin D3. (c-kitinhibitors.com)
Human pregnane X receptor (PXR) is a major nuclear receptor that upregulates the expression of drug-metabolizing enzymes such as CYP3A4. (aspetjournals.org)
Epigenomic Factors in Human Obesity16. (ra-berg.de)
Recently, the development of the HepG2-NTCP sec+ cell line, expressing the sodium taurocholate co-transporting polypeptide receptor (NTCP) and self-assembling co-cultured primary human hepatocytes (SACC-PHHs) has opened new perspectives for a better understanding of HBV and HDV lifecycles and the development of specific antiviral drug targets against HBV/HDV infections. (cdc.gov)

Primary hepatocytes isolated from a transgenic mouse line containing the mouse tTG promoter driving the beta-galactosidase reporter, show similar time-dependent increases in promoter activity when treated with TNF-alpha. (nih.gov)

The factors that govern this specificity of UGT expression remain largely unknown. (aspetjournals.org)
Previously, it was shown that mesenchymal to epithelial transition (MET) is promoted by repression of the zinc finger E-box-binding 1 (ZEB1) and ZEB2 expression [ 19 , 20 ]. (oncotarget.com)
The direct interconnection between CR and circadian rhythms has been evinced by the fact that Igf-1 expression is regulated by both CR and the circadian clock [ 14 ] . (encyclopedia.pub)
Interestingly, Igf-1 expression is rhythmic and shows sexual dimorphism [ 14 ] . (encyclopedia.pub)
Insulin secreted upon refeeding-after-fasting stimulates Per2 and reduces Rev-erbα expression in hepatocytes [ 18 ] . (encyclopedia.pub)
In this study, we aimed to explore the effect of NFAT2 expression on cell fate of HepG2 cell and its potential mechanisms. (researchsquare.com)

Induction of canonical autophagy is mainly controlled by the AKT/mTOR and AMPK signaling pathways and relies on the activation and assembly of two macromolecular complexes, the ATG1/ULK1 and the Class III phosphatidylinositol 3-kinase (PI3K) complex (Figure 1 ) [ 5 ]. (hindawi.com)

As of January 1, 2022, Oncotarget has shifted to a continuous publishing model. (oncotarget.com)

These results would imply that nLD are actively involved in nuclear processes that include lipids. (unlp.edu.ar)
2017. Networks of enzymatically oxidized membrane lipids support calcium-dependent coagulation factor binding to maintain hemostasis . (lsrnw.ac.uk)
Lipids in Health and Disease, 19 (1). (well-labs.com)

Conversely, higher levels of BA and cyclooxygenase-2 (COX-2) are risk factors for inflammation and cancer of the colon. (cancerindex.org)

Recently, a molecular evolution study of the HERV-W provirus in several ape species and 24 humans demonstrated the conservation of syncytin-1, as well as its retention of receptor-mediated fusogenic activity [ 14 ]. (ecancer.org)

the latter is necessary for hepatic transcription of PEPCK-C. AP-1 competed with HNF4α for binding to this site and also decreased HNF4α stimulation of transcription from the PEPCK-C gene promoter. (elsevierpure.com)
Hepatic fibrosis in rats was induced by the intra-peritoneal injection of NDEA (10 mlkg −1 b.wt. (nature.com)
Adipocytes are an important component of hepatic microenvironment in nonalcoholic fatty liver disease (NAFLD), which is a significant risk factor for HCC. (biomedcentral.com)

Please see Additional file 1 for translations of the abstract into the six official working languages of the United Nations. (biomedcentral.com)

MSCs can promote host tissue repair through several different mechanisms including donor cell engraftment, release of cell signaling factors, and the transfer of healthy organelles to the host. (frontiersin.org)

We propose a therapeutic regimen to supplement the current supportive therapy aiming to reduce viral load, the most important factor in the determination of mortality. (biomedcentral.com)

The "Multiple Hit Theory" is based on the interaction between genetic and/or environmental factors, and changes in crosstalk between different organs and tissues. (biomedcentral.com)

Solute carrier family 22 member 1 (Organic cation transporter 1) (hOCT1). (tcdb.org)
17] "Novel single nucleotide polymorphisms of organic cation transporter 1 (SLC22A1) affecting transport functions. (tcdb.org)

Like all viruses, the EBOV largely relies on host cell factors and physiological processes for its entry, replication, and egress. (biomedcentral.com)

Transposable elements (TEs) or mobile elements are genetic components that can have a significant effect on the host genome [ 1 , 2 ]. (ecancer.org)
Many environmental, physiological and genetic factors have been implicated in defective sperm function, the most common cause of infertility. (propoleo.cl)

Taken together, these results demonstrated a differential response by HCV 3a genotype in HCV-induced pathogenesis, which may be due to Core and host factor Cox-2 individually or in combination. (biomedcentral.com)

[ 2 , 3 ] The software application uses quantitative analysis of nuclear medicine liver-spleen images to determine the severity of liver disease and to predict clinical outcomes. (medscape.com)

Accordingly, the impact of CR on plasma IGF-1 and insulin level is compromised in mice deficient for Bmal1 [ 16 ] . (encyclopedia.pub)

Anatomy20

Organisms8

Diseases6

Chemicals and Drugs97

Analytical, Diagnostic and Therapeutic Techniques and Equipment24

Phenomena and Processes53

Disciplines and Occupations1

Information Science3

Health Care1

Treating HepG2 cells1

Hepatocellular carcinoma3

Apoptosis4

Cells36

Transcription Factors6

Steatosis3

Induces1

Lipid1

Proteins3

Apolipoprotein1

Receptors4

MRNA3

Vivo1

Exosomes4

Translocation1

Mutations1

Transcriptional3

Human10

Primary1

Expression6

Pathways1

20221

Lipids3

Inflammation1

Receptor-mediated1

Hepatic3

Abstract1

Mechanisms1

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Crosstalk1

Transporter2

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Mice1