runx1 expression in the lateral plate mesoderm co-localizes with the hematopoietic transcription factor scl , and expression of runx1 is markedly reduced in the zebrafish mutants spadetail and cloche . (biologists.com)
Development2
While this role has been best documented in the developmental migration of the neural crest and mesoderm, here we expand on previously reported preliminary findings that morpholino knock-down of snai2 impairs the generation of hematopoietic stem cells (HSCs) during zebrafish development. (bvsalud.org)
moreover, they also promote the hematopoietic differentiation of mouse ESCs and favor the development of definitive HSCs from pre-HSCs in the mouse aorta-gonad-mesonephros (AGM) region [ 8 ]. (biomedcentral.com)
Results1
Nature Medicine, 2014) and was subsequently able to show that ribosomal protein haploinsufficiency results in selective impairment of GATA1 mRNA translation (Ludwig et al. (simplyblood.org)
Mouse1
Description: The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). (thermofisher.com)
Expression3
Snail2 is a zinc-finger transcription factor best known to repress expression of genes encoding cell adherence proteins to facilitate induction of the epithelial-to-mesenchymal transition. (bvsalud.org)
The expression levels of VGLL3 mRNA and protein were analyzed in the freshly resected tumor and normal gastric tissues from GC patients by quantitative RT-PCR and Western blot, respectively. (researchgate.net)
Multivariate analysis showed that VGLL3 expression status was an independent risk factor for prognosis. (researchgate.net)
Differentiation7
2. Dynamics of the epigenetic landscape during erythroid differentiation after GATA1 restoration. (nih.gov)
8. A regulatory network governing Gata1 and Gata2 gene transcription orchestrates erythroid lineage differentiation. (nih.gov)
15. Hypoxia-inducible factor 1-mediated human GATA1 induction promotes erythroid differentiation under hypoxic conditions. (nih.gov)
Dysfunction and abnormal differentiation of immune cells and hematopoietic precursors are well described in patients with cancer, although the role of transcription factors in these defects is not well established. (scientificarchives.com)
Thus, it is conceivable to speculate that an abnormal expression of proto-oncogenes in lymphoid cells and myeloid cells may be due to a general imbalance of transcription factors responsible for the lineage-specific differentiation. (scientificarchives.com)
Leukocytes' fate is progressively determined during the gradual differentiation of hematopoietic stem cells (HSCs) into multipotent progenitors (MPPs) and common lymphoid or myeloid progenitors [ 8 ]. (scientificarchives.com)
9. CDC6 expression is regulated by lineage-specific transcription factor GATA1. (nih.gov)
Transcriptional5
16. Transcriptional regulation by GATA1 and GATA2 during erythropoiesis. (nih.gov)
17. Insight into GATA1 transcriptional activity through interrogation of cis elements disrupted in human erythroid disorders. (nih.gov)
20 kb on average) with exceptionally higher (as compared to binding to typical enhancers) binding of transcriptional coactivators, such as mediator or EP300, or BRD4, or CDK7 [ 14 ], and have high potential to activate transcription of their target genes (Fig. 1 ). (biomedcentral.com)
The C-terminal domain is required for the transcriptional activation of the sodium-dependent bile acid co-transporter (NTCP) gene and is therefore likely to be required for the activation of transcription in other contexts [ 28 , 29 ]. (biomedcentral.com)
These results open a new opportunity to test whether altered transcriptional regulation of mature immune cells in cancer may be intrinsically related to abnormal functioning of transcription factors in early hematopoiesis or occurs independently via direct effects of tumor-derived factors on circulating immune cells. (scientificarchives.com)
Hematopoietic4
Hematopoietic stem cells (HSCs) are the source of all blood cells over an individual's lifetime. (bvsalud.org)
Antisense morpholino-mediated knockdown of the Integrator subunit 5 (Ints5) in zebrafish embryos affects U1 and U2 snRNA processing, leading to aberrant splicing of smad1 and smad5 RNA, and reduced expression of the hematopoietic genes stem cell leukemia ( scl , also known as tal1 ) and gata1 . (biologists.com)
Analysis of expression of transcription factors in CD34+CD45dim hematopoietic precursors showed that in contrast to the downregulation of transcription factor in mature peripheral blood immune cells, expression of these regulators in stem/precursor cells was significantly increased in patients with lung cancer, especially in patients with detectable metastases. (scientificarchives.com)
Transcription factors are key regulators of these cells acting through their capacity to bind DNA and control gene transcription, which thus regulates the development of specific hematopoietic lineages from stem cells. (scientificarchives.com)
Gene7
3. Global transcriptome and chromatin occupancy analysis reveal the short isoform of GATA1 is deficient for erythroid specification and gene expression. (nih.gov)
6. Analysis of disease-causing GATA1 mutations in murine gene complementation systems. (nih.gov)
13. The Gata1 5' region harbors distinct cis-regulatory modules that direct gene activation in erythroid cells and gene inactivation in HSCs. (nih.gov)
Diseased HSCs can be replaced with gene-engineered or healthy HSCs through HSC transplantation (HSCT). (bvsalud.org)
Enhancer is a class of regulatory DNA sequence that activates transcription of an associated gene from a distance of up to 1 Mbps (millions of base pairs) and independent of its orientation and location with respect to the transcription start sites (TSS) [ 1 ]. (biomedcentral.com)
The proline rich homeodomain protein/haematopoietically expressed homeobox (PRH/HHEX), is a transcription factor encoded by the HHEX gene [ 1 - 3 ]. (biomedcentral.com)
C/EBPΑ (CCAAT/enhancer binding protein (C/EBP) alpha), a protein encoded by the C/EBPΑ gene in humans, was originally identified 30 years ago as a transcription factor that binds both promoter and enhancer regions. (scientificarchives.com)
Abnormal transcription1
Cancer cells acquire super-enhancers at oncogene and cancerous phenotype relies on these abnormal transcription propelled by SEs. (biomedcentral.com)
Stem1
We developed CD117/LNP-messenger RNA (mRNA), a lipid nanoparticle (LNP) that encapsulates mRNA and is targeted to the stem cell factor receptor (CD117) on HSCs. (bvsalud.org)
Regulate2
PRH can also bind to other transcription factors and co-regulate specific target genes either directly through DNA binding, or indirectly through effects on the activity of its partner proteins. (biomedcentral.com)
Hematopoiesis, the dynamic process of blood cell development, is regulated by the activity of the bone morphogenetic protein (BMP) signaling pathway and by many transcription factors. (biologists.com)
Regulatory1
7. Meis1, Hi1α, and GATA1 are integrated into a hierarchical regulatory network to mediate primitive erythropoiesis. (nih.gov)
This binding also affects the three-dimensional structure of DNA, allowing the interaction between the activators and enhancers, the transcription factors, as well as the core promoter region and the RNA polymerase [ 2 ]. (biomedcentral.com)
Enhancer1
The identification of super-enhancer relies mainly on the chromatin immunoprecipitation (ChIP)-sequencing analysis using a combination of active enhancer marks (H3K27ac, H3K4me1), co-activators, and transcription factor profiles (especially cell-type defining TFs). (biomedcentral.com)
Protein4
Transient myeloproliferative disorder (TMD) is a pre-leukemic condition that occurs only in neonates with Trisomy 21 (T21), and is attributed to a genetic interaction between the third copy of chromosome 21 (HSA21) and a mutation in the transcription factor GATA1 that results in a truncated protein (GATA1s). (bvsalud.org)
Transient myeloproliferative disorder (TMD) is a pre-leukemic condition that occurs only in neonates with DS and is characterized by a mutation in the transcription factor GATA1 that results in a truncated protein (GATA1s). (bvsalud.org)
The proline rich homeodomain protein (PRH), also known as haematopoietically expressed homeobox (HHEX), is an essential transcription factor in embryonic development and in the adult. (biomedcentral.com)
This is consistent with the involvement of multiple PRH-interacting proteins, including the oncoprotein c-Myc, translation initiation factor 4E (eIF4E), and the promyelocytic leukaemia protein (PML), in the control of cell proliferation and cell survival. (biomedcentral.com)
Function3
4. Chromatin occupancy and epigenetic analysis reveal new insights into the function of the GATA1 N terminus in erythropoiesis. (nih.gov)
Furthermore, in vivo delivery of pro-apoptotic PUMA (p53 up-regulated modulator of apoptosis) mRNA with CD117/LNP affected HSC function and permitted nongenotoxic conditioning for HSCT. (bvsalud.org)
The ability to target HSCs in vivo offers a nongenotoxic conditioning regimen for HSCT, and this platform could be the basis of in vivo genome editing to cure genetic disorders, which would abrogate the need for HSCT. (bvsalud.org)
Similarly, multiple PRH target genes, including the genes encoding vascular endothelial growth factor (VEGF), VEGF receptors, Endoglin, and Goosecoid, are known to be important in the control of cell proliferation and cell survival and/or the regulation of cell migration and invasion. (biomedcentral.com)
Levels1
10. Regulation of GATA1 levels in erythropoiesis. (nih.gov)
Cells1
Immune abnormalities in cancer may be due to the effect of tumor/stroma-derived factors on mature circulating immune cells or on their precursors in the bone marrow. (scientificarchives.com)