DRB1AntibodyAntigensEpitopesMoleculesAntibodiesAllelesAntigenGenotypesHaplotypesGeneHeterodimericProteinLociPolymorphismsAssociationsHumanMoleculeCell surfaceCellsBeta chainPublicationsIndividualsAntigenBelongs to the HLA classDRB3ParaloguesAnchored in the membraneGenesPeptideFetalGeneticSusceptibilityHeavy chainAlpha chainPatients
DRB18
- DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. (wikipedia.org)
- Allelic variants of DRB1 are linked with either none or one of the genes DRB3, DRB4 and DRB5. (wikipedia.org)
- DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. (thermofisher.com)
- Western Blot: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Western Blot Analysis of Ramos cell lysate using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067). (novusbio.com)
- Immunohistochemistry-Paraffin: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Human Tonsil stained with HLA-DRB Monoclonal Antibody (LN-3 + HLA-DRB/1067). (novusbio.com)
- Flow Cytometry: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Flow Cytometric Analysis of Human Raji cells using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067). (novusbio.com)
- This monoclonal antibody reacts with the beta-chain of HLA-DRB1 antigen, a member of MHC class II molecules. (novusbio.com)
- The polymorphisms associated with the "shared epitope" are located on the α-helical rim (DRB1 chain) of the peptide-binding cleft, where they may interact with either the bound peptide antigen or the T cell receptor. (musculoskeletalkey.com)
Antibody6
- The following product was used in this experiment: HLA-DR Monoclonal Antibody (LN3), Super Bright™ 436, eBioscience™ from Thermo Fisher Scientific, catalog # 62-9956-42, RRID AB_2744822. (thermofisher.com)
- As the complexity of the HLA system defined by antibody reagents grew, the Cell Exchange focused its efforts on providing challenging cell types - those that included "rare" HLA antigens or unusual combinations of antigens that might be difficult to discriminate. (uclahealth.org)
- A serum exchange was added in 1981, to check for anti-HLA antibody specificities. (uclahealth.org)
- for example the MHC Class II antibody based on clone IVA12 recognizes the shared epitopes of human leucocyte antigen (HLA) class II molecules HLA-DP, HLA-DQ and HLA-DR heterodimeric cell surface glycoproteins comprised of an α (heavy) chain and a β (light) chain. (antibodies-online.com)
- DSA-FXM: Accelerated donor specific flow crossmatch discriminating class I and II antibody specifically and only to donor HLA for determining true incompatibility. (stanford.edu)
- Interestingly, the recent data in RA indicate that the major HLA-DR associations are with anti-CCP antibody positive disease, suggesting that control of autoantibody responses may be a primary mechanism underlying these associations in RA as well. (musculoskeletalkey.com)
Antigens4
- HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. (novusbio.com)
- In the absence of autoantibodies, an incompatible crossmatch in a sensitized patient is attributed to mismatched donor human leukocyte antigens (HLA). (stanford.edu)
- Result interpretation is difficult or impossible when autoantibodies, alloantibodies, and/or therapeutic antibodies coexist.METHODS: Herein we describe a unique donor specific flow crossmatch (DSA-FXM) that distinguishes HLA class I and/or II DSA bound to HLA antigens on the donor cell surface in their native conformation that is not impacted by rituximab, ATG (after absorption), or auto-antibodies. (stanford.edu)
- In each individual, T cells are generally restricted to recognize antigens presented by the person's own HLA molecules. (musculoskeletalkey.com)
Epitopes2
- Mapping and definition of HLA class I and II serologic epitopes using an unbiased reverse engineering strategy. (stanford.edu)
- Current models describing HLA epitopes are both theoretical and empirical. (stanford.edu)
Molecules7
- HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). (thermofisher.com)
- In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. (novusbio.com)
- The HLA molecules and their counterparts in rodents were subsequently shown to be directly responsible for immune response differences between individuals and for determining the likelihood of graft rejection. (musculoskeletalkey.com)
- The allelic variations among different HLA molecules are a major factor accounting for differences in the types of antigenic peptides to which an individual responds or in the types of T cells that are used in an immune response. (musculoskeletalkey.com)
- HLA class I molecules consist of a 45-kD α chain encoded within the MHC that is noncovalently associated with the 12-kD β 2 -microglobulin chain (encoded on chromosome 15). (musculoskeletalkey.com)
- HLA class II molecules consist of noncovalently associated α (32 kD) and β (28 kD) chains, both of which are encoded within the MHC. (musculoskeletalkey.com)
- HLA class I and class II molecules are cell surface glycoproteins, anchored to the membrane by hydrophobic transmembrane segments. (musculoskeletalkey.com)
Antibodies2
- The accuracy of these reagents was dependent upon local methods of assessing the specificities of the anti-HLA antibodies and the volumes made it difficult to distribute sufficient quantities for use by everyone who performed HLA testing. (uclahealth.org)
- Browse our portfolio of HLA antibodies down below! (antibodies-online.com)
Alleles1
- In the case of systemic lupus erythematosus (SLE) and related illnesses, many of the HLA class II alleles are associated with the presence of specific autoantibodies or clinical phenotypes. (musculoskeletalkey.com)
Antigen6
- Description: The LN3 mAb reacts with the human major histocompatibility complex (MHC) class II, HLA-DR. HLA-DR is expressed on the surface of human antigen presenting cells (APC) including B cells, monocytes, macrophages, DCs, and activated T cells. (thermofisher.com)
- HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. (thermofisher.com)
- In naïve DA rats IgE, the bone marrow stromal cell antigen 1 ( Bst1 ) and the MHC class II β-chain ( MhcII ) were expressed at a higher level, and the immunoglobulin kappa chain ( Igκ ) was expressed at a lower level. (biomedcentral.com)
- In humans, MHC proteins are encoded by the Human Leukocyte Antigen (HLA), a group of more than 200 genes located closely together on the short arm of chromosome 6. (antibodies-online.com)
- Classical class I and class II Human Leukocyte Antigen (HLA) are leading candidates for infectious disease susceptibility. (antibodies-online.com)
- We examined the relationships of human leukocyte antigen (HLA) and related polymorphisms to glioblastoma multiforme in adult Caucasians (non-Hispanic Whites) from the San Francisco Bay area. (aacrjournals.org)
Genotypes1
- Thus, both the occurrence and the prognosis of glioblastoma multiforme could be associated with specific but different HLA genotypes. (aacrjournals.org)
Haplotypes1
- Association of various HLA haplotypes with SARS-CoV-2 infection and the course of COVID-19 could improve assessment of viral severity in the population. (antibodies-online.com)
Gene4
- The protein encoded by this gene belongs to the HLA class II beta chain paralogues. (wikipedia.org)
- The alpha chain gene contains 5 exons. (thermofisher.com)
- The beta chain is approximately 26-28 kDa and its gene contains 6 exons. (solarbio.cc)
- This gene encodes the type I intermediate filament chain keratin 17, expressed in nail bed, hair follicle, sebaceous glands, and other epidermal appendages. (creativebiomart.net)
Heterodimeric2
- HLA-DR is a heterodimeric transmembrane protein composed of alpha and beta subunits and plays an important role in the presentation of peptides to CD4+ T lymphocytes. (thermofisher.com)
- HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. (novusbio.com)
Protein4
- Recombinant protein encompassing a sequence within the center region of human HLA DR beta. (antibodies-online.cn)
- The specificity of T-cells is determined by the T-cell receptor (TCR), a heterodimer of alpha and beta protein chains. (elifesciences.org)
- These include the formation of disulphide bonds between the sulphydryl groups on cysteine residues, hydrogen bonding between different side chain groups, and hydrophobic interactions that result in hydrophobic side chains becoming protected within the interior of the protein. (biozol.de)
- Proteins which are composed of multiple polypeptide chains exhibit quaternary structure, which is a term that is used to refer to the manner in which the different subunits interact with one another to form a larger protein complex. (biozol.de)
Loci1
- Many observations point to a major role for classical HLA loci in determining susceptibility to viral infections 1 . (antibodies-online.com)
Polymorphisms1
- Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. (wikipedia.org)
Associations1
- Class II HLA Serotype Associations with End-Stage Renal Disease due to Membranous Nephropathy among Caucasians in the United States Charu, V., Tyan, D., Troxell, M., Kambham, N. NATURE PUBLISHING GROUP. (stanford.edu)
Human1
- A different allele, HLA-B*15:03, showed the greatest capacity to present highly conserved SARS-CoV-2 peptides that are shared among common human coronaviruses, suggesting it could enable cross-protective T-cell based immunity. (antibodies-online.com)
Molecule2
- The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. (wikipedia.org)
- In February 2007, a pilot study for MICA (class I chain-related molecule A) typing was initiated by sending samples to a select number of laboratories. (uclahealth.org)
Cell surface1
- However, current physical crossmatch methods cannot distinguish reactivity to HLA from other clinically irrelevant cell surface targets nor the class of HLA if it is the target. (stanford.edu)
Cells1
- The Cell Exchange provided an alternative strategy, distributing the same cells to many laboratories for HLA typing. (uclahealth.org)
Beta chain2
- The beta chain is approximately 26-28 kDa. (wikipedia.org)
- HLA-DRB4 belongs to the HLA class II beta chain paralogues. (antibodies-online.cn)
Publications1
- The UCLA International Cell Exchange has recorded the progress of HLA typing in a number of publications and presentations at national and international meetings. (uclahealth.org)
Individuals1
- A recent study shows that individuals with the allele HLA-B*46:01 have the fewest predicted binding peptides for SARS-CoV-2, suggesting they may be particularly vulnerable to COVID-19, as they were previously shown to be for SARS. (antibodies-online.com)
Antigen4
- Segregation analyses in several populations have suggested a relationship between specific human leukocyte antigen (HLA) class II alleles and the development of different types of leprosy. (nih.gov)
- Human platelet antigen alleles in 998 Taiwanese blood donors determined by sequence-specific primer polymerase chain reaction. (cdc.gov)
- HLA-DRB3*01:01 is a predictor of immunization against human platelet antigen-1a but not of the severity of fetal and neonatal alloimmune thrombocytopenia. (cdc.gov)
- Combination of HLA-DR on Mycobacterium tuberculosis -Specific Cells and Tuberculosis Antigen/Phytohemagglutinin Ratio for Discriminating Active Tuberculosis From Latent Tuberculosis Infection. (nih.gov)
Belongs to the HLA class2
- The protein encoded by this gene belongs to the HLA class II beta chain paralogues. (wikipedia.org)
- HLA-G belongs to the HLA class I heavy chain paralogues. (antikoerper-online.de)
DRB33
- Compound heterozygosity of HLA-DRB3*01:01 and HLA-DRB4*01:01 as a potential predictor of fetal neonatal alloimmune thrombocytopenia. (cdc.gov)
- HLA-DRB3*01:01 exhibits a dose-dependent impact on HPA-1a antibody levels in HPA-1a-immunized women. (cdc.gov)
- 13. Next-Generation Sequencing Reveals That HLA-DRB3, -DRB4, and -DRB5 May Be Associated With Islet Autoantibodies and Risk for Childhood Type 1 Diabetes. (nih.gov)
Paralogues1
- HLA-DRA is one of the HLA class II alpha chain paralogues. (nih.gov)
Anchored in the membrane2
- The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. (wikipedia.org)
- The heavy chain is anchored in the membrane. (antikoerper-online.de)
Genes3
- However, the presence on the cell surface of MHC class II molecules of several HLA allotypes derived from different genes, and the high degree of polymorphism of these genes, results in several different peptide specificities within an individual and within the human population as a whole. (medscape.com)
- HLA class II molecules are composed of two variable chains, α and β, encoded by three groups of genes, DR, DP and DQ. (medscape.com)
- 10. Non-HLA genes PTPN22, CDK6 and PADI4 are associated with specific autoantibodies in HLA-defined subgroups of rheumatoid arthritis. (nih.gov)
Peptide2
- Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. (wikipedia.org)
- HLA-DR molecules are chaperoned from the endoplasmic reticulum to the MHC class II-containing compartment by the nonpolymorphic invariant chain that is subsequently degraded except for the peptide CLIP, which remains in the peptide-binding groove of DR. A second chaperone, HLA-DM, functions to support the exchange of CLIP for a high-affinity peptide in the peptide-binding groove. (medscape.com)
Fetal2
- Maternal HLA genotyping is not useful for predicting severity of fetal and neonatal alloimmune thrombocytopenia. (cdc.gov)
- HLA-G is expressed on fetal derived placental cells. (antikoerper-online.de)
Genetic2
Susceptibility1
- Tri-SNP polymorphism in the intron of HLA-DRA1 affects type 1 diabetes susceptibility in the Finnish population. (nih.gov)
Heavy chain1
- The heavy chain is approximately 45 kDa and its gene contains 8 exons. (antikoerper-online.de)
Patients2
- Study of the HLA associations in patients not carrying these heterodimers has been limited by the rarity of such patients. (nih.gov)
- 15. Antibodies specific to ferritin light chain polypeptide are frequently detected in patients with immune‑related pancytopenia. (nih.gov)