I was wondering if anyone has used a glutathione analog in which the sulfhydral group is blocked to elude bound GST-fusion proteins from a GSH-Sepharose Chromatography Column (Pharmacia). (bio.net)
About a 40-fold purification of the cyclase supernatant protein was achieved by a combination of (NH4)2SO4 fractionation and phenyl-Sepharose chromatography. (docksci.com)
Recently, it was demonstrated that vaccination of cattle with Sera products of adult worms enriched for cysteine proteinases by thiol-Sepharose chromatography reduced the fecal egg counts by 60% compared to the counts in the control group (11). (massorrerjazzbar.com)
In this basic research and experimental study, at first, DNA that encodes recombinant PE38 protein was inductively expressed in Escherichia coli (E.coli) and purified by nickel-sepharose chromatography and further analyzed by western blot. (celljournal.org)
This process involves the use of protein G beads or sepharose, and it finds its application in the powerful technique known as affinity chromatography. (lovewholesome.com)
In affinity chromatography, a mixture containing the protein of interest is passed through a column packed with protein G beads or sepharose. (lovewholesome.com)
DEAE-Sepharose4
DEAE-Sepharose is a tradename for the anion-exchange reactive group, diethylaminoethanol (DEAE) covalently linked to Sepharose (a polysaccharide polymer). (wikipedia.org)
Sephacryl S-300 and DEAE-Sepharose Fast Flow cellulose were provided by GE Healthcare Life Sciences. (springeropen.com)
After re-dissolution, the ZMP was added to a DEAE-Sepharose FF column equalized with 0.4 M NaCl and a Sephacryl S-300 column balanced with distilled water. (springeropen.com)
A polyphenol oxidase catalyzing 3¢-hydroxylation of larreatricin to 3¢-hydroxylarreatricin was purified from the leaves of creosote bush by DEAE-Sepharose, Hydroxylapatite, and Phenyl Sepharose column chromatographies. (khugene.com)
Heparin sepharose2
All anti prion polyanions seem to inhibit the binding of PrPc to heparinsepharose, demonstrating a clear connection between the heparin binding activity of PrPc and inhibition of prion replication. (europa.eu)
Crotamine was purified to homogeneity in a single step procedure using HeparinSepharose. (scite.ai)
Covalently2
The Glycosorb ABO immunoadsorption column contains ABO blood group antigens covalently bound to sepharose particles. (apollohospitals.com)
Protein G-Sepharose beads (Cat# M1301) for IgG purification contain covalently coupled recombinant Protein G to 6% cross-linked Sepharose beads, the most popular resin for protein purification. (transcriptionfactor.org)
Proteins4
Ni Sepharose High Performance precharged with Ni2+ for high-performance purification of histidine-tagged proteins. (sigmaaldrich.com)
Sepharose CL-6B resulted in a significantly higher number of separated EVs while lipoproteins were eluted together with free proteins. (izon.com)
The peptide showed antibacterial activity on Escherichia coli (MIC: 2.0 μg/μL), Staphylococcus aureus (MIC: 8-16 μg/μL) and methicillin-resistant Staphylococcus aureus (MIC: 4.0-8.0 μg/μL), inducing bacterial death by lipid peroxidation and oxidation of target proteins, determined by thiobarbituric acid reactive substances and sulfhydryl groups, respectively. (scite.ai)
The GST-c-Fos fusion healthy proteins indicated in bacteria were purified by glutathione-Sepharose 4B (GE Healthcare) or nickel-nitrilotriacetic acid-agarose beads (Qiagen). (techblessing.com)
Beads3
Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µM) to which a chelating group has been immobilized and subsequently charged with Ni 2+ ions. (sigmaaldrich.com)
In addition, it uses a spin column approach, offering significant speed, process and carry-over advantages over sepharose or magnetic beads. (labbulletin.com)
The protein G beads or sepharose have a high affinity for antibodies, allowing them to selectively bind to the target protein. (lovewholesome.com)
Ammonium1
Gamma globulin (mostly IgG) fraction of the sera was precipitated by half saturation of ammonium sulfate and linked to activated sepharose 4B. (ac.ir)
Conjugate3
Immunoprecipitation of NIH/3T3 cell lysates, untreated or PDGF-treated, using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (Sepharose ® Bead Conjugate) compared to supernatants collected after immunoprecipitation from the same lysate. (cellsignal.jp)
The performance of these matrices for the purification of the major rat GSTs was compared with a matrix that used a GSH-BSP conjugate ligand, also attached to epichlorhydrin-activated Sepharose. (wgtn.ac.nz)
The glutathione-requiring dehalogenating activity bound only to the GSH-BSP conjugate ligand attached to epichlorhydrin-activated Sepharose. (wgtn.ac.nz)
Fractionation1
In cell fractionation research, the worth under each street in the Traditional western mark images shows the comparable sums of protein comparable to the control group. (techblessing.com)
Purification3
ToxOut™ Protein G (Sepharose) Antibody Purification Kit is a ready-to-use kit for endotoxin-free IgG purification and immunoprecipitation. (transcriptionfactor.org)
Types of affinity matrix for the purification of the glutathione S-transferases and glutathione-requiring dehalogenases Glutathione and S-hexyl glutathione were attached directly to epichlorhydrin-activated Sepharose such that there was no spacer group between the ligand and the matrix. (wgtn.ac.nz)
4] the SDS-PAGE music group appearance Traditional western blotting and amino acidity analysis show the salivary mucins which eluted in the void and included amounts from the Sepharose CL-4B gel purification column to become MUC5B and MUC7 respectively. (techblessing.com)
Procedure2
This sample preparation procedure is applicable for all formats containing Ni Sepharose High Performance ( Cell Lysis ). (sigmaaldrich.com)
overcome by using a highly selective procedure to remove the potentially damaging blood group antibodies and slow their production during the critical post operative period. (apollohospitals.com)
Ligand1
Protein G, derived from the cell wall of group G Streptococcus bacteria, is an ideal ligand for purifying antibodies due to its ability to bind to the constant region of immunoglobulins from various species. (lovewholesome.com)
Molecular mass1
The cyclase supernatant protein(s) was not internalized by Sephadex G-50 and did not bind to Blue Sepharose, suggesting that it has a molecular mass of over 30 kDa and that it does not bind the cofactor NADPH. (docksci.com)
Column2
A pool of nine CSF samples was separated by SEC columns packed with Sepharose CL-6B, Sephacryl S-400 or Superose 6PG and a ready-to-use qEV10/70 nm column. (izon.com)
Analysis of 2GPI binding with molecules on THP-1 cell membrane To investigate whether 2GPI can bind to the specific molecules on THP-1 cell surface membrane, the 2GPI-affinity column (2GPI-Affi-Gel) was first prepared using cyanogen bromide (CNBr)-triggered Sepharose? (iassist2012.org)
Concomitant methotrexate use was lower in the group with anti-adalimumab antibodies (52%) than in the group without antibodies (84%) (p = 0.003). (bmj.com)
Bound1
The cyclase supernatant protein did bind to MgProtoMe2-bound Sepharose and could be eluted by raising the pH to 9.7 in the presence of 4 mM-n-octyl glucoside. (docksci.com)
Streptococcus2
In Group A Streptococcus (GAS)-infected macrophages, Txnip was degraded independent of glucose consumption and streptococcal cysteine protease expression. (frontiersin.org)
Group A Streptococcus (GAS) infection causes various diseases ranging from mild pharyngitis and impetigo to severe necrotizing fasciitis and streptococcal toxic shock syndrome (STSS) ( 4 ). (frontiersin.org)
Columns3
The first ABO-incompatible kidney transplant at the Apollo hospitals Chennai was done by using the Immune adsorption columns to reduce the specific blood group antibody titer before the ABO-incompatible transplant. (apollohospitals.com)
By using SEC with different columns, we have found that although a minor fraction of exosomes can be isolated without significant albumin content on Sepharose CL-4B or Sephacryl S-400 columns, but not on Sepharose 2B columns, the majority of exosomes co-eluted with albumin. (nih.gov)
Human gallbladder bile was spiked with [ 3 H]cholesterol and [ 14 C]phospholipid and applied to two sets of Con A- Sepharose columns. (wustl.edu)
Suitable1
Our data indicate that Sepharose CL-6B is suitable for isolation of EVs from CSF and their separation from lipoproteins. (izon.com)
Spacer2
Group Z is a spacer selected from (CH2)n and CH2O(CH2CH2O)n2, wherein n is an integer of from 1 to 5, and wherein n2 is an integer of from 1 to 4. (justia.com)
Group Z2 and Z3 is a spacer and need not be the same. (justia.com)
High5
Ni Sepharose High Performance is compatible with all commonly used aqueous buffers, reducing agents, and denaturants such as 6 M Gua-HCl and 8 M urea, as well as a range of other additives, and allows thorough procedures for cleaning the medium ( Characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products ). (sigmaaldrich.com)
The good flow properties and high resolution make Ni Sepharose High Performance the choice for high-performance purifications for the main characteristics of Ni Sepharose High Performance. (sigmaaldrich.com)
Ni Sepharose High Performance is supplied preswollen in 20% ethanol, in pack sizes of 25 and 100 mL, as well as in the convenient prepacked formats described later in this chapter. (sigmaaldrich.com)
0.001) and rather high after 90 years compared to the other groups. (go.jp)
TBM should be a strong consideration when a patient presents with a clinical picture of meningoencephalitides, especially in high-risk groups. (medscape.com)
Glutathione1
In addition to base assisted deprotonation of glutathione, it is proposed that a positively charged group in the GST active site assists in the leaving of the chloride ion from CDNB. (wgtn.ac.nz)
Amino1
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. (lookformedical.com)
Specific1
IT treatment significantly inhibited proliferation of HUVEC and MCF-7 in a VEGFR2-specific manner as compared with the control groups (p (celljournal.org)
Technology1
Launched worldwide in 2012 - Chromatrap® solid-state ChIP technology has been shown by a growing number of research groups worldwide to be more efficient than conventional bead-based methods. (labbulletin.com)
Compatible1
Limitations in donor availability for organ transplantation makes ""living donor"" solid organ transplantation across the blood group barrier to become an alternative to ABO compatible transplantation for those with end stage renal disease facing long waiting times. (apollohospitals.com)
Control group1
The geometric mean cumulative fecal egg counts in the nOPA-vaccinated animals were reduced by 60% compared to the counts in the control group during the 2-month PI3k-delta inhibitor 1 course of the experiment. (massorrerjazzbar.com)
Active1
Polysaccharide is one of the most abundant components of jujube, and it represents a major group of biolotegically active constituents. (springeropen.com)
Risk2
Transplantation of solid organs like kidney between donors and recipients of incompatible blood group is usually contraindicated because of the risk of ""hyper acute"" rejection. (apollohospitals.com)
The last CVAI quartile group (Q4) presented the highest risk of T2DM (OR, 2.17, 95% CI, 1.67-2.83), after adjusting for all covariates. (bvsalud.org)