Gram rna bacterial. Medical search. Frequent questions

It is active against a wide range of gram-positive bacteria including methicillin-resistant Staphylococcus aureus (MRSA). (nih.gov)
It is also active against certain gram-negative bacteria. (nih.gov)
C72H85N19O18S5) is a thiazoline antibiotic that is effective against Gram-positive bacteria and the malarial parasite, Plasmodium falciparum. (uwaterloo.ca)
In vitro studies of rifaximin have demonstrated broad-spectrum coverage including gram-positive, gram-negative, and anaerobic bacteria as well as a limited risk of bacterial resistance [ 5 ]. (hindawi.com)
The NEBNext rRNA Depletion Kit (bacteria) removes the abundant bacterial rRNAs (5S, 16S, and 23S) resulting in the enrichment of bacterial mRNAs and non-coding RNAs of interest. (genomeweb.com)
The NEBNext ® rRNA Depletion Kit (Bacteria) employs the NEBNext RNase H-based RNA depletion workflow to target removal of rRNA from gram-positive and gram-negative organisms. (neb.com)
The great majority of RNA in bacteria is ribosomal RNA (rRNA). (neb.com)
Ribosomal RNA was depleted using the NEBNext rRNA Depletion Kit (Bacteria). (neb.com)
Demand for linezolid is set to increase at the highest CAGR over the forecast period with a broad spectrum of activity against many gram-positive bacteria. (factmr.com)
Vancomycin does not have any effect on gram-negative bacteria, mycobacteria, or even fungi in vitro. (factmr.com)
Together, the data suggest that caloric restriction enhances resistance to gram-positive bacteria, while lowering the production of proinflammatory mediators elicited by endotoxin, a component of gram-negative bacteria. (cdc.gov)
Pseudomonas aeruginosa is the gram negative rod shaped bacteria is found in all natural and man made environments. (ukessays.com)
The bacteria is gram negative, rod shaped and aerobic belongs to the bacterial family of pseudomonadanceae. (ukessays.com)
We determined whether Gram-negative bacterial molecules are associated with Alzheimer disease (AD) neuropathology given that previous studies demonstrate Gram-negative Escherichia coli bacteria can form extracellular amyloid and Gram-negative bacteria have been reported as the predominant bacteria found in normal human brains. (neurology.org)
Its overall accuracy is 93.6% when compared to the accuracy of 16S Ribosomal RNA gene sequencing, the gold standard for identifying anaerobic bacteria, Enterobacteriaceae, Gram-positive aerobes, fastidious Gram-negative bacteria, Gram-negative non-Enterobacteriaceae, and yeast. (aacc.org)
Correlation of in vitro transcription inhibition activity with in vivo mechanism of action was established using fluorescence microscopy and resistance passaging using Gram-positive bacteria showed no resistance development over 30 days. (edu.au)
Nitrofurantoin is a bacteriostatic, nitrofuran antibiotic targeting gram-negative and gram-positive bacteria such as Enterococci , Staphylococci , Streptococci, Corynebacterium , and E. Coli . (goldbio.com)
They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. (goldbio.com)
Outer membrane vesicles (OMVs) are released by commensal as well as pathogenic Gram-negative bacteria. (uu.nl)
These studies demonstrate for the first time that pathogenic bacteria on the nebulizer surface pose a risk of bacterial inhalation to patients who use contaminated nebulizers. (bvsalud.org)
The RNeasy Protect Mini Kit and Bacteria Mini Kit include RNeasy spin columns for purifying up to 100 µg of high-quality RNA using silica-membrane technology. (qiagen.com)
Antimicrobial analysis and choosing an appropriate antibiotic based on the type of bacteria being treated are essential for successfully controlling bacterial diseases. (azooptics.com)
Gram-negative bacteria are the most frequently discovered pathogens in 75% to 90% of UTI cases. (azooptics.com)
The ProQ/FinO family of RNA binding proteins mediate sRNA-directed gene regulation throughout gram-negative bacteria. (nature.com)
Despite phage replication in these matrices, lysis of the bacteria was slower than phage propagation, and no reduction of the bacterial population was observed. (bvsalud.org)
Daptomycin is primarily active against gram-positive bacteria, and is clinically used to treat complicated infections with drug resistant bacteria, like MRSA or VRE infections. (pixorize.com)
Daptomycin is a natural product that has clinical utility in the treatment of infections caused by aerobic Gram-positive bacteria. (globalrph.com)
The in vitro spectrum of activity of daptomycin encompasses most clinically relevant Gram-positive pathogenic bacteria. (globalrph.com)
Daptomycin retains potency against antibiotic-resistant Gram-positive bacteria, including isolates resistant to methicillin, vancomycin, and linezolid. (globalrph.com)
Bacterial 16S ribosomal RNA (rRNA) genes contain nine "hyper-variable regions" (V1-V9) that demonstrate considerable sequence diversity among different bacteria. (academicjournals.org)
In conclusion, the DNA sequencing based method provides a valuable tool for cheap and accurate diagnosis of Gram-negative bacteria in urinary tract infections which can be applicable in other infections and in the diagnosis of other pathogenic bacteria. (academicjournals.org)
We used small subunit ribosomal RNA (16S rRNA) gene DNA data to identify bacteria from ten sites. (abrinternationaljournal.org)
The phenotypic analysis of the unknown samples identified rod-shaped Gram-negative bacteria under a microscope. (abrinternationaljournal.org)
The R2014 Zymo Quick-RNA Fungal/Bacterial Microprep Kit is designed for the simple and rapid isolation of RNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa. (mbpinc.net)
Gram-negative bacteria are protected by a three-layer cell envelope composed of a cell wall sandwiched between two membranes. (viennabiocenter.org)
In terms of their mode of action against gram-positive vs gram-negative bacteria, it has been observed that sulphonamides are less effective against gram-negative bacteria as they have an additional outer membrane that makes it difficult for the drug to gain access to the bacterial cell wall. (recnotes.com)
Firstly, urinary tract infections are typically caused by gram-negative bacteria, like Escherichia coli. (recnotes.com)
Secondly, respiratory tract infections are typically caused by both gram-positive and gram-negative bacteria. (recnotes.com)
Overall, sulphonamides are an effective class of drugs for treating bacterial infections caused by susceptible bacteria, particularly urinary tract infections, respiratory tract infections, and meningitis. (recnotes.com)
These may include a bacteria culture , viral blood tests, and a Gram stain . (medlineplus.gov)
Flowchart that can be used to identify bacteria by using Gram-positive stain. (cdc.gov)
T3SS is an essential component for the virulence of pathogenic Gram-negative bacteria, such as Salmonella and Chlamydia. (lu.se)

Numerous sRNAs have been identified using both computational analysis and laboratory-based techniques such as Northern blotting, microarrays and RNA-Seq in a number of bacterial species including Escherichia coli, the model pathogen Salmonella, the nitrogen-fixing alphaproteobacterium Sinorhizobium meliloti, marine cyanobacteria, Francisella tularensis (the causative agent of tularaemia), Streptococcus pyogenes, the pathogen Staphylococcus aureus, and the plant pathogen Xanthomonas oryzae pathovar oryzae. (wikipedia.org)
Indicated for adults and pediatric patients aged 3 months or older for complicated intra-abdominal infections (cIAIs) in combination with metronidazole and for complicated urinary tract infections (cUTIs) including pyelonephritis caused by certain susceptible Gram-negative microorganisms, including Escherichia coli . (medscape.com)
2 mo), Staphylococcus aureus is the most common cause of septic arthritis (SA), but Escherichia coli, group B streptococci, and other gram-negative bacilli also cause the disease. (medscape.com)
It is also active against gram-negative pathogens, such as Escherichia coli and Haemophilus influenzae. (com.bd)
Boris Görke's lab has now identified that in Escherichia coli an RNA binding protein senses and regulates synthesis of an important precursor of the bacterial cell envelope. (viennabiocenter.org)

The 6S RNA binds to RNA polymerase and regulates transcription, tmRNA has functions in protein synthesis, including the recycling of stalled ribosomes, 4.5S RNA regulates signal recognition particle (SRP), which is required for the secretion of proteins and RNase P is involved in maturing tRNAs. (wikipedia.org)
Binds to beta-subunit of bacterial DNA-dependent RNA polymerase, thereby inhibiting RNA synthesis. (medscape.com)
Mupirocin inhibits bacterial protein synthesis by reversibly and specifically binding to bacterial isoleucyl transfer-RNA synthetase. (nih.gov)
Rifaximin binds to the beta-subunit of bacterial DNA-dependent RNA polymerase and prevents catalysis of polymerization of deoxyribonucleotides into a DNA strand, thereby inhibiting bacterial RNA synthesis. (hindawi.com)
It also has an impact on bacterial cell membrane permeability and RNA synthesis. (factmr.com)
Nitrofurantoin functions by inactivating bacterial ribosomal proteins causing inhibition of DNA, RNA, protein, and cell wall synthesis. (goldbio.com)
In addition, vancomycin alters bacterial-cell-membrane permeability and RNA synthesis. (pfizermedicalinformation.com)
Here we show that staphylococcal phages produce a structured RNA transcribed from the terminase subunit genes, termed CBASS-activating bacteriophage RNA (cabRNA), which binds to a positively charged surface of the CdnE03 cyclase and promotes the synthesis of the cyclic dinucleotide cGAMP to activate the CBASS immune response. (bvsalud.org)
Other projects on Gram-positive cell-wall biosynthesis, carbohydrate degradation, bacterial RNA synthesis, and barnacle settlement cues. (ncl.ac.uk)
This inhibits bacterial protein synthesis. (healthguidenet.com)
Sulphonamides work by hindering bacterial folic acid synthesis, which is essential for the survival of a bacterium. (recnotes.com)
Their mechanism of action involves the inhibition of bacterial folic acid synthesis, which is essential for bacterial growth and replication. (recnotes.com)
Interestingly, sulphonamides selectively target bacterial folic acid synthesis, meaning that they don't harm human cells which do not synthesize folic acid. (recnotes.com)
Sulphonamides work by inhibiting the bacterial folic acid synthesis, which is essential for the synthesis of DNA and RNA. (recnotes.com)
Mupirocin inhibits bacterial RNA and protein synthesis. (msdmanuals.com)

The VITEK MS can detect nearly 200 different microorganisms that represent the majority of bacterial and fungal infections that afflict humans, yielding results in minutes. (aacc.org)
The emergence of antimicrobial resistance severely threatens our ability to treat bacterial infections. (frontiersin.org)
An important concept in the evaluation of data regarding transfusion-transmitted bacterial infections (TTBIs) is the definition of a case. (medscape.com)
Individuals with CF are prone to lung infections due to inefficient bacterial clearance and by the age of 18, eighty percent of patients with CF harbor Pseudomonas aeruginosa , with it being the most prevalent respiratory microorganism at that age. (umaine.edu)
When it comes to fighting bacterial infections, sulphonamides have been around the block a few times. (recnotes.com)
Sulphonamides, commonly known as sulfa drugs, are a class of antibiotics used to treat bacterial infections. (recnotes.com)
Sulphonamides are a class of drugs that are used to treat a variety of bacterial infections. (recnotes.com)
Staphylococcal Infections Staphylococci are gram-positive aerobic organisms. (msdmanuals.com)
Streptococcal Infections Streptococci are gram-positive aerobic organisms that cause many disorders, including pharyngitis, pneumonia, wound and skin infections, sepsis, and endocarditis. (msdmanuals.com)
Other viral and bacterial tests for respiratory infections are often limited to testing for one specific pathogen. (medlineplus.gov)
The Aa is also known as Haemophillus actimycetemcomitans, and is also responsible for a series of systemic infections, including the bacterial endocarditis, the brain and skin abscesses and the urinary tract 39,47,48 . (bvsalud.org)

Bacterial sRNAs affect how genes are expressed within bacterial cells via interaction with mRNA or protein, and thus can affect a variety of bacterial functions like metabolism, virulence, environmental stress response, and structure. (wikipedia.org)
Easy-to-use AllPrep Bacterial/Fungal DNA/RNA/Protein Kits isolate total nucleic acids and cellular proteins from Gram (+/-) bacterial cultures and fungal cell cultures. (qiagen.com)
DNA and RNA are ready for qPCR, RT-PCR and next-generation sequencing, while protein is ready for 1D SDS-PAGE and mass spectrometry following in-gel trypsin digestion. (qiagen.com)
As in previous studies, several circular replication-associated protein encoding (CRESS) DNA virus-like sequences were recovered in the blank control libraries, as well as contaminating sequences from the Totiviridae, Tombusviridae and Lentiviridae families of RNA virus. (bvsalud.org)
sRNAs usually work by pairing with target mRNAs, often with the assistance of protein partners called RNA chaperones. (nature.com)
Similarly, a minimal ProQ/FinO domain protein, NMB1681, has been shown to bind a range of structured RNAs in Neisseria meningitidis 15 . (nature.com)
One is that prions are infectious protein particles, not having associated DNA/RNA (evidence is sensitivity to proteases, insensitivity to nucleases), the other is prions are a name for something no-one has a clue about. (bio.net)

Ribosomal RNA (rRNA) is highly abundant in bacterial samples, and its removal is desirable in order to reveal the biological significance of less abundant transcripts. (neb.com)
The recent developments in the taxonomy based on the 16s ribosomal RNA or conserved macromolecules, the family members are divided into eight groups. (ukessays.com)
ribosomal RNA identification of the isolates showed high score similarity with Pseudomonas spp. (lu.se)
Some of the enzymes which generate aromatic radicals that break down most prominent bacterial strains found were isolated and tax- the complex linkages present in lignin to compounds of lower onomically identified using 16S ribosomal RNA (rRNA) se- molecular weight. (lu.se)

Specific enrichment of bacterial mRNAs is challenging due to their lack of poly(A) tails, and so the converse - efficient and specific removal of bacterial rRNA - is necessary. (neb.com)
Effective depletion of sequences overlapping with annotated rRNA regions was observed at 100 ng and 10 ng of input RNA for most of the organisms. (neb.com)
Observation of tiny colonies of Gram-positive, catalase-negative coccoid rods triggered molecular identification based on 16S rRNA gene sequencing. (biomedcentral.com)

Bacterial endotoxin-stimulated in vitro production of nitric oxide and tumor necrosis factor (TNF)-alpha as well as expression of TNF-alpha and interleukin-6 messenger RNAs were all lower in alveolar macrophages isolated from calorie-restricted rats. (cdc.gov)
In-vitro Antibacterial Activity of a Fosfomycin-Trimethoprim-Sulfamethoxazole Combination Against a Bacterial Collection of Multidrug-Resistant Clinical Isolates. (asm.org)
Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. (goldbio.com)
We also provide results of an in vitro assay conducted to monitor bacterial surface detachment during multiple cycles of rewetting that correlate with the results of nebulizer/bacterial surface interactions. (bvsalud.org)
Vancomycin is not active in vitro against gram-negative bacilli, mycobacteria, or fungi. (pfizermedicalinformation.com)
Consistent with these in vitro findings, phage treatment did not reduce bacterial burdens in a neutropenic mouse S. aureus thigh infection model. (bvsalud.org)
Daptomycin exhibits rapid, concentration-dependent bactericidal activity against Gram-positive organisms in vitro. (globalrph.com)

In molecular biology, generalized transduction is routinely used as a technique to manipulate and construct bacterial strains. (bvsalud.org)
In addition, the zebrafish's optical clarity allows for the use of fluorescently-labeled bacterial strains to track and image infection in live zebrafish embryos. (umaine.edu)
We determined that the 167 nucleotides-long V6 region is not suitable for distinguishing all bacterial strains to the genus and species level and could not distinguish among all bacterial species especially Klebsiella strains. (academicjournals.org)
Staphylococcus aureus (a Gram-positive, round-shaped bacterium), including methicillin-resistant strains. (healthguidenet.com)

0.4%), broad-spectrum antibiotic specific for enteric pathogens of the GI tract (ie, gram-positive, gram-negative, aerobic and anaerobic). (medscape.com)
Flowchart of Gram stain morphology that can be used to identify aerobic Gram-positive cocci. (cdc.gov)

Detection of Actinobaculum schaalii in clinical isolates mainly reflects infection indicating that this Gram-positive rod is not an innocent bystander. (biomedcentral.com)
Bacterial metabolism selected lignin model compounds (vanillin, vanillate, 4- hydroxybenzoate, p-coumarate, benzoate, and ferulate) was carried out with three of the Pseudomonas isolates and in addition with the strain Pseudomonas putida KT2440 from a Introduction culture collection. (lu.se)

I am looking for a protocol and/or reference for total RNA isolation from E. coli cells. (bio.net)
DNA binds to the first spin column and the flow-through is combined with a solution to bind the total RNA on a second spin column. (qiagen.com)
The new kits complement the NEBNext products for RNA library preparation, including the Ultra II Directional RNA Library Prep Kit for Illumina and the NEBNext Library Quant Kit for Illumina, as well as the Monarch Total RNA Miniprep Kit, the company said. (genomeweb.com)
Total RNA was extracted from a lyophilized pool of 20 different bacterial organisms (ATCC® #MSA-2002). (neb.com)
Using total RNA sequencing ("metatranscriptomics") we documented the presence of contaminant viral sequences in multiple 'blank' negative control sequencing libraries that comprise a sterile water and reagent mix. (bvsalud.org)
The RNeasy Protect Cell Mini Kit provides the RNeasy Plus Mini Kit for purification of total RNA. (qiagen.com)
The RNeasy Protect Saliva Mini Kit includes the RNeasy Micro Kit, which purifies and concentrates total RNA using specialized RNeasy MinElute spin columns. (qiagen.com)

The objectives of this study were to (i) determine whether different pathogens detach and disperse from the nebulizer surface during aerosolization and (ii) measure the effects of relative humidity and drying times on bacterial surface detachment and aerosolization. (bvsalud.org)
We also focused on how much sequencing information is needed for blind identification of bacterial pathogens. (academicjournals.org)

Inhibitors of bacterial RNA polymerase transcription complex" by Daniel S. Wenholz, Michael Miller et al. (edu.au)
A series of hybrid compounds that incorporated anthranilic acid with activated 1H-indoles through a glyoxylamide linker were designed to target bacterial RNA polymerase holoenzyme formation using computational docking. (edu.au)

Epiglottitis, also termed supraglottitis or epiglottiditis, is an inflammation of structures above the insertion of the glottis and is most often caused by bacterial infection. (medscape.com)
Although viruses normally do not cause epiglottitis, a previous viral infection may allow bacterial superinfection to occur. (medscape.com)
How bacterial cyclases recognize phage infection is not known. (bvsalud.org)
It is important to research the effects of CF, P . aeruginosa , and arsenic to understand how they are modulating the immune system and to potentially discover novel therapies to combat CF and bacterial infection. (umaine.edu)
With the use of the zebrafish and high-throughput sequencing, the lab aims to aid in the discovery of novel therapies for people suffering from cystic fibrosis and bacterial infection. (umaine.edu)
All in all, sulphonamides are still quite vital when it comes to treating a bacterial infection or two. (recnotes.com)

Clinical laboratories have embraced molecular biology tools in recent years, including RNA gene sequencing, PCR-based methods, and biosensors to facilitate rapid and accurate identification of a specific pathogen involved in UTIs. (azooptics.com)

This method is targeted to both gram-positive and gram-negative organisms, and is effective with RNA from monocultures or mixed bacterial species, such as those used in metatranscriptome studies, NEB said. (genomeweb.com)
The method is effective with intact and degraded RNA, from monocultures or samples with mixed bacterial species (e.g., metatranscriptomic). (neb.com)
Numerous studies have confirmed that AIEC are enriched in humans with CD compared to healthy subjects and are often the dominant bacterial species present 15 , 16 . (nature.com)
Small RNAs (sRNAs) control a variety of physiological responses across bacterial species 1 . (nature.com)

A CSF culture with Gram stain did not show bacterial growth. (cdc.gov)
This result shows that although gram stain could not detect many intact cells in the liquid culture, cells were not dead. (figshare.com)
Phenotypic traits such as Gram stain, colony shape, growth on various nutrient mediums, and other biochemical assays are the basis for identification and classification in clinical laboratories. (azooptics.com)

they are 50- to 500-nucleotide non-coding RNA molecules, highly structured and containing several stem-loops. (wikipedia.org)
The data show that Gram-negative bacterial molecules are associated with AD neuropathology. (neurology.org)

It is concentrated in the urine and active against most gram-positive and some gram-negative organisms. (medscape.com)
Bacitracin is produced by Bacillus subtilis and has a potent bactericidal activity directed mainly against Gram-positive organisms. (healthguidenet.com)

The RNA-stabilizing properties of RNAprotect Tissue Reagent prevent gene induction or down-regulation triggered by sample manipulation allowing you to preserve and analyze the gene expression profile. (qiagen.com)

Cultured bacterial and fungal cells are first lysed using glass bead tubes, and nucleic acids and proteins are solubilized and mixed with a binding solution. (qiagen.com)
The bacterial chromosomic DNA is packed within a membrane-less structure, the nucleoid, due to the association of DNA with proteins called Nucleoid Associated Proteins (NAPs). (cea.fr)
Here, we investigate the structural basis for RNA recognition by ProQ/FinO proteins, through the crystal structure of the ProQ/FinO domain of the Legionella pneumophila DNA uptake regulator, RocC, bound to the transcriptional terminator of its primary partner, the sRNA RocR. (nature.com)
More recently, the application of RNA-seq technologies has enabled the elucidation of the biological partners and targets of several ProQ/FinO family proteins. (nature.com)
Insight into how these proteins recognize their cognate RNAs initiated with FinO. (nature.com)
Bacterial spore proteins which get produced in the presence of chloramphenicol seems to be a throw-back to this principle. (bio.net)
We sought to determine if there was antibody deposition in SAH livers and whether antibodies extracted from SAH livers were cross-reactive against both bacterial antigens and human proteins. (elifesciences.org)

A structure activity relationship study identified the key structural components necessary for inhibition of both bacterial growth and transcription. (edu.au)

As the mammalian cyclase OAS1 also binds viral double-stranded RNA during the interferon response, our results reveal a conserved mechanism for the activation of innate antiviral defence pathways. (bvsalud.org)

It is now known that most bacterial sRNAs are encoded by free-standing genes located in the intergenic regions (IGR) between two known genes. (wikipedia.org)
The Kim lab recently completed RNA sequencing analysis to measure the dysregulation of genes in response to these factors in zebrafish embryos. (umaine.edu)

P . aeruginosa is a gram negative, opportunistic bacterium that is found all over the planet. (umaine.edu)
It is also called pseudomonic acid thanks to its major metabolite, derived from submerged fermentation of Pseudomonas fluorescens (a common Gram-negative, rod-shaped bacterium). (healthguidenet.com)

Actinobaculum schaalii is a Gram-positive, facultative anaerobic coccoid rod, classified as a new genus in 1997. (biomedcentral.com)

Each gram of Mupirocin Ointment USP, 2% contains 20 mg mupirocin in a bland water miscible ointment base (polyethylene glycol ointment, N.F.) consisting of polyethylene glycol 400 and polyethylene glycol 3350. (nih.gov)
Mupirocin Ointment USP, 2% is available as follows:22 gram tube (NDC 45802-112-22)Store at 20-25C (68-77F) [see USP Controlled Room Temperature].Bactroban Nasal(R) is registered trademark of GlaxoSmithKline.MADE IN ISRAELMANUFACTURED BY PERRIGO, YERUHAM 80500, ISRAELRepacked by: H.J. Harkins Company, Inc.Nipomo, CA 93444Rev: 11/11. (drugcentral.org)

AllPrep Bacterial/Fungal. (qiagen.com)
The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state-of-the-art, ultra-high-density BashingBeads. (mbpinc.net)

Structural analysis and RNA binding studies reveal that other ProQ/FinO domains also recognize related transcriptional terminators with different specificities for the length of the 3' ssRNA tail. (nature.com)
Another plasmid-encoded ProQ/FinO family member, FopA, has also been shown to interact with a single antisense RNA 11 . (nature.com)

Daptomycin is an antibiotic that kills bacterial cells by disrupting the cell membrane. (pixorize.com)

The small RNA ryfA has been found to affect the stress response of uropathogenic E.coli, under osmotic and oxidative stress. (wikipedia.org)
Available at http://www.who.int/vaccine_research/diseases/e_e_coli/en/ . (medscape.com)

Therefore, we describe here an isolation method of ultrapure OMVs and the subsequent extraction of RNA and basic steps of RNA-Seq analysis. (uu.nl)

Bacterial culture, extracellular supernatant concentration, OMV purification, and the subsequent RNA extraction out of OMVs are described. (uu.nl)

He identified periodic acid-Schiff (PAS)-staining granules, most likely representing degenerating bacterial forms, within macrophages isolated from the small bowel as well as other tissue and fluid specimens (eg, pericardium, endocardium, lymph nodes, synovia, lung, brain, meninges) obtained from patients in whom Whipple disease was suspected. (medscape.com)

In the current study, ten spots in the caves were screened to identify bacterial communities, and two unknown samples were subjected to phenotypic and molecular identification. (abrinternationaljournal.org)
Zymo-Spin column technology is then used to isolate the RNA that is ideal for downstream molecular-based applications including Next-Gen sequencing, RT-PCR, microarray, hybridization, etc. (mbpinc.net)

Northern blotting can reveal possible sRNA transcript size and expression levels by running a mixed RNA sample on an agarose gel and probing for a desired sRNA. (wikipedia.org)

Since these initial discoveries, over six thousand bacterial sRNAs have been identified, largely through RNA-sequencing experiments. (wikipedia.org)
RNA-sequencing, or RNA-seq, is used to analyze expression levels of all transcripts in a genome, including sRNAs. (wikipedia.org)
Bacterial sRNAs have a wide variety of regulatory mechanisms. (wikipedia.org)

It prevents N-acetylmuramic acid and N-acetylglucosamine peptide subunits from being incorporated into the peptidoglycan matrix, the most critical structural component of Gram-positive cell walls. (factmr.com)

Standard biochemical and phenotypic techniques such as culture and gram staining are crucial for a clinical microbiology laboratory. (azooptics.com)

MALDI-TOF MS is a practical, quick, simple-to-use, and relatively inexpensive technique for bacterial identification compared to traditional approaches. (azooptics.com)

Mupirocin is mainly efficacious against Gram-positive aerobes and works by binding to bacterial isoleucyl transfer RNA synthetase. (healthguidenet.com)

The Gram-negative coccus that causes the disease is called Neisseria gonorrhoeae . (pediagenosis.com)

A Randomized, Double-Blind, Placebo Controlled Multicenter Study to Evaluate the Efficacy and Safety of CAL02 Administered Intravenously in Addition to Standard of Care in Subjects With Severe Community Acquired Bacterial Pneumonia (SCABP). (asm.org)

Although increased bacterial resistance is considered beneficial, reduction in the lung's ability to induce inflammatory mediators can have both positive and pathophysiologic consequences. (cdc.gov)
Exploring the Effect of Sub-MIC Antibiotic Exposure on Bacterial Hetero-resistance at Single Cell Level. (asm.org)

The estimated residual risk of contamination of blood products with bacterial agents is 1 in 5,000 for platelets and 1 in 30,000 for red blood cells. (medscape.com)
Also important is the duration of storage, which has a direct correlation with the likelihood of bacterial contamination. (medscape.com)
A meta-analysis of 22 studies of bacterial contamination rate estimates for apheresis (AP), platelet-rich plasma (PRP), and buffy coat (BC) collection methods found an overall mean contamination rate of 0.51 per 1000 components (95% confidence index [CI], 0.38-0.67). (medscape.com)
Probable contamination - The blood culture from the recipient is negative or could not be done, but there is definite bacterial growth in the donor blood product. (medscape.com)
Specific pitfalls within the protocol and RNA contamination sources are highlighted. (uu.nl)

This highly abundant RNA can conceal the biological significance of less abundant transcripts, and so its efficient and specific removal is desirable. (neb.com)

Indeed, Hfq is best known to mediate post-transcriptional regulation by using small noncoding RNA (sRNA). (cea.fr)

Anatomy15

Organisms29

Diseases5

Chemicals and Drugs114

Analytical, Diagnostic and Therapeutic Techniques and Equipment28

Phenomena and Processes70

Information Science4

Named Groups1

Health Care4

Bacteria42

Escherichia5

Synthesis15

Infections11

Protein7

Ribosomal RNA4

RRNA3

Vitro7

Strains4

Aerobic2

Isolates2

Total RNA7

Pathogens2

Polymerase2

Infection6

Pathogen1

Species4

Stain3

Molecules2

Organisms2

Gene1

Proteins7

Inhibition1

Binds1

Genes2

Bacterium2

Facultative anaerobic1

Mupirocin Ointment USP2

Fungal2

ProQ2

Cell membrane1

Coli2

Isolation1

Extracellular1

Macrophages isolated1

Molecular2

Agarose gel1

SRNAs3

Structural1

Microbiology1

Identification1

Aerobes1

Neisseria1

Pneumonia1

Resistance2

Contamination5

Abundant1

Mediate1