• Some discrepancy was observed comparing G2P prediction of the molecular clones and their coreceptor usage CONCLUSIONS: The recombinant phenotypic assay described is highly comparable to ESTA moreover it offers the possibility to dissect the composite phenotype of viral quasispecie at the clonal level providing information about the influence of specific aminoacidic residue. (univpm.it)
  • Main objectives of the present project are: i) to broaden the molecular knowledge on the AADC and antiquitin deficiencies, ii) to interlink knowledges (levels of neurotransmitters, coenzymes, metabolites) about AADC, PNPO and antiquitin patients (phenotype) with related enzyme activities (from genotype) and iii) to develop new therapeutic strategies. (univr.it)
  • METHODS: Recombinant DNA techniques were used to introduce a blunt-end cloning cassette in the replicating HIV-NL4-3 molecular clone in order to clone different V3 sequences from patient virus in the replicative backbone for phenotypic testing. (univpm.it)
  • The experimental methods are multiple: molecular biology techniques, a combination of bioinformatic analyses with physico-chemical biochemical methods both in solution with recombinant proteins and validated in cell models. (univr.it)