Genomic proteins expressed sequence tags. Medical search. Frequent questions

Four predicted genes (CDS1-CDS4) CDS that have been confirmed on the mRNA level showed no similarity to known proteins and a potential function could not be assigned. (nih.gov)

The Helminth Secretome Database (HSD) is a repository for helminth proteins predicted using expressed sequence tags (ESTs). (wikipedia.org)
Previously identified ESTs, which correspond to known helminth proteins, are used to predict the location and function of newly discovered helminth proteins based on genomic sequencing. (wikipedia.org)
Aligning expressed sequence tags (ESTs) to the genomic sequence has become a popular approach for gene prediction, revealing alternative splice variants. (diva-portal.org)
Similarity searches of nucleotide and protein sequence databases revealed some evidence of homology with a number of expressed sequence tags (ESTs) and a Caenorhabditis elegans protein, which shows a slight similarity with Arabidopsis thaliana ubiquitin-like protein 8 [ 8 ] . (medscape.com)
Amino acid sequences deduced from human and mouse ESTs showed 100% homology with the P . obesus beacon, indicating that this protein is highly conserved between species. (medscape.com)
This highlights the important role of ESTs for generating genetic and genomic resources of aquaculture species. (marbigen.org)
In this paper, haplotype-based SNPs were mined out of publicly availablecitrus expressed sequence tags (ESTs) from different citrus cultivars(genotypes) individually and collectively for comparison. (biomedcentral.com)
Among these, 52 non redundant DEGs were subjected to BLAST analysis for similarity search using BioEdit local blast tool (E-values 1.0E-50) against total ESTs, genomic survey sequences (GSS) and nucleotide sequences of pigeonpea downloaded from NCBI. (longdom.org)
Cluster analysis of 1630 valid ESTs enabled the identification of 178 groups of related sequences and 871 singlets. (unige.ch)
Approximately 50% of the putative unique 1059 ESTs could be annotated using Blast searches against the protein database SwissProt + TrEMBL. (unige.ch)
Translation of ESTs can provide partial aa sequences of the proteome. (rndsystems.com)
It really is popular that the era of large-scale indicated sequenced tags (ESTs) can be an extremely useful method of explain the gene manifestation profile and series of mRNA from a particular organism and stage (specifically in non-model varieties). (bioxorio.com)
Currently, only the grasses surpass the legumes for the number of publicly available expressed sequence tags (ESTs). (umn.edu)
To begin to decipher the molecular processes underlying gene function, we constructed a database of expressed sequence tags (ESTs) generated from sparganum. (biomedcentral.com)
For contemporary functional genomic studies, the significant efforts invested in the creation of integrative transcript databases of expressed sequence tags (ESTs) derived from full-length cDNA libraries have provided opportunities to direct gene discovery and functional analysis. (biomedcentral.com)
First, the mouse tsg101 cDNA sequence was used to query the Expressed Sequences Tags (ESTs) database of the National Center for Biotechnology Information (NCBI) by the BLAST program in order to find homologous sequences in the human genome. (davidson.edu)

Human DNA sequence corresponding to the beacon gene was recently mapped to chromosome 19 (Genbank accession no. (medscape.com)
The complete genomic sequence of the isolate, designated J1 (GenBank accession no. (cdc.gov)
Recombinant histidine-tagged WU-VP1 protein was generated by expressing WU-VP1 (GenBank accession no. (cdc.gov)
Previously existing sequence data were restricted to around 22,000 expressed sequence tag (EST) sequences and ~ 1,500 GenBank sequences. (edu.pe)
Three years ago, NCBI's Carolyn Tolstoshev and I founded the database of expressed sequence tags (dbEST) which is a division of GenBank for cDNA sequence and mapping data. (nih.gov)
When new sequences are submitted to GenBank, the submission is checked for new organism names, which are then classified and added to the Taxonomy database. (nih.gov)

It was possible to confirm by interspecies mRNA-to-genomic alignments 25 and 21 alternative splice events in sea bream and sea bass genes, respectively. (marbigen.org)
Nonsense-mediated mRNA decay (NMD) downmodulates mRNAs that have in-frame premature termination codons and prevents translation of potentially harmful truncated proteins from aberrant mRNAs. (biomedcentral.com)
A role for NMD in the regulation of mRNA metabolism beyond the mere vacuum cleaner function for faulty mRNAs has been suspected, and was foreshadowed by work on the splicing factor SC35 and some ribosomal proteins [ 6 , 7 ]. (biomedcentral.com)
8 ] used high-density oligonucleotide arrays to analyze genome-wide expression profiles of yeast strains containing single deletions of the UPF1 , UPF2 or UPF3 genes, as well as of the DCP1 and XRN1 genes which encode proteins with activities thought to be involved in the NMD pathway - an essential component of the mRNA decapping enzyme and the 5'-3' exonuclease, respectively. (biomedcentral.com)
Expression of genes occurs through transcription - the assembly of continuous, single stranded, messenger ribonucleic acid (mRNA) copies of exons, which are translated into proteins. (ddw-online.com)
Credits The mRNA track was produced at UCSC from mRNA sequence data submitted to the international public sequence databases by scientists worldwide. (ucsc.edu)
A 3-base sequence in a tRNA molecule that base-pairs with its complementary codon in an mRNA molecule. (bscs.org)
They can be used experimentally to determine the sequence of an mRNA. (bscs.org)
Although IME1 mRNA is observed in vegetative cultures, Ime1 protein is not suggesting the presence of a regulatory system restricting translation to meiotic cells. (biomedcentral.com)
This study demonstrates that IME1 mRNA translation requires Rpl22A and Rpl22B, eukaryotic-specific ribosomal protein paralogs of the 60S large subunit. (biomedcentral.com)
In addition, a set of subgenomic mRNAs encoding structural and accessory proteins is produced by a sophisticated mechanism involving nested mRNA synthesis using the full-length genomic RNA as template. (iucr.org)

The sequences presented here will provide much needed genomic resources for three distinct but overlapping fields of inquiry: neurobiology, speciation, and molecular evolution. (nih.gov)

One of the first applications of Generation II sequencing has been maturation of reference genome sequences and variation catalogues, with compilation variant frequencies by sequencing of many human genomes (resequencing) (116-119). (ddw-online.com)
Comparisons of the available sequenced genomes suggest a minimum gene set for vascular plants of 4,223 genes. (biomedcentral.com)
To examine the molecular evolution of NCX genes and understand the role of duplicated genes in the evolution of the vertebrate NCX gene family, we carried out phylogenetic analyses of NCX genes and compared NCX gene structures from sequenced genomes and individual clones. (biomedcentral.com)
Duplicates for NCX1 , NCX2 , and NCX4 were found in all sequenced teleost genomes. (biomedcentral.com)

Thus, NMD serves as a 'cellular vacuum cleaner' that protects the cell from the potentially harmful effects of truncated proteins by eliminating mRNAs with PTCs in a sequence of events that is not yet fully understood. (biomedcentral.com)
Single or simultaneous inactivation of the UPF genes stabilizes nonsense-containing mRNAs, indicating that their protein products interact functionally in the same pathway. (biomedcentral.com)
More intriguing is the discovery of several new classes of NMD targets, including mRNAs that use translational +1 frameshifting, bicistronic mRNAs and, most interestingly, two classes of noncoding RNAs: pseudogene transcripts and transcripts encoded by transposable elements or their long terminal repeat (LTR) sequences. (biomedcentral.com)
Protein diversity largely results from multiple, alternative ways in which exons are assembled into mRNAs (alternative splicing) (120). (ddw-online.com)
For example, to apply the filter to all mRNAs expressed in a specific organ, type the name of the organ in the tissue box. (ucsc.edu)
This track may also be configured to display codon coloring, a feature that allows the user to quickly compare mRNAs against the genomic sequence. (ucsc.edu)

The availability of genomic resources for sweetpotato is in striking contrast to its importance for human nutrition. (edu.pe)
The availability of genomic databases will no doubt lead to the discovery of additional chemokines, particularly those that display very restricted expression patterns. (rndsystems.com)

The comparative annotation approach represents a good strategy for transferring more relevant biological information from highly studied species to genomic resource poorer species. (marbigen.org)
Biological content was enriched by reconciling all available cDNA and expressed sequence tag data with gene predictions, clarifying single nucleotide polymorphism and RNAi sites, and summarizing known functions for most genes studied in this organism. (nih.gov)
The earliest discoveries of chemokines were made as a result of their biological activity or through studies that sought to identify proteins that are upregulated following cell activation or differentially expressed in selected cell types. (rndsystems.com)
2003) GoMiner: a resource for biological interpretation of genomic and proteomic data, Genome Biol. (insilicogen.com)
Therefore, the motivation for this project was to develop a database and tool that would provide parasite researchers with flexible access to parasite-specific information about sparganum that is relevant for studying the production of parasite proteins and identifying molecules involved in key biological pathways that might serve as targets for diagnostic markers and treatments for the control of sparganum. (biomedcentral.com)
Darren has had many years' experience of using sequence data to answer biological questions. (exeter.ac.uk)

There are two known proteins with chemokine homology that fall outside of the α and ß subfamilies. (rndsystems.com)
As a working definition, putatively legume-specific genes had no sequence homology, below a specified threshold, to publicly available sequences of nonlegumes. (umn.edu)
A query of the NCBI database returned no human gene sequences that had significant homology to the TSG101 gene, supporting the notion that the gene is a unique human homologue to the mouse tsg101. (davidson.edu)
Inheritance, homology by common descent, and the conservation of sequence and structure in the determination of function are all central ideas in biology that are directly related to the evolutionary history of any group of organisms. (nih.gov)
Although chIL-36RN exhibits low homology with its mammalian orthologues, it encodes a predicted beta-trefoil structure whose beta-strands are conserved Citarinostat molecular weight with those of the mouse sequence. (peptidesolubility.com)

This integrative web-based database of sequence data, functional annotations and expression profiling data will serve as a useful tool to help understand and expand the characterization of parasitic infections. (biomedcentral.com)
Identification and genomic characterization of major effect bacterial blight resistance locus (BB-13) in Upland cotton (Gossypium hirsutum L. (ncsu.edu)

The second region in 7q22 defined by the CUTL1 (CCAAT displacement protein and its splice variant) locus is 416 kb in length and contains three known genes, including PMSL12, APS, CUTL1, and a novel gene (CDS5). (nih.gov)
n\nChicken IL-36RN, like chIL-1RN, is not encoded at the chIL-1 beta locus, further emphasising the genomic fragmentation of the large IL-1 gene cluster found in mammals. (peptidesolubility.com)
Although it is possible to sequence all genes within a linked locus using high-throughput second-generation sequencing, it is important to prioritize any identified sequence changes for further follow-up. (molvis.org)

NCBI's Gene resources include collections of curated nucleotide sequences used as references, sequence clusters to predict and study homologs, and various databases and tools for the study of gene expression. (nih.gov)

The sequences reported in this paper have been deposited in the NCBI database, under accession numbers HS514072-HS519705. (biomedcentral.com)
cDNA sequences are aligned to release 6 by NCBI and submitted to FlyBase. (flybase.org)
To add to the mix, in 1990 the NCBI and the NLM initiated a journal-scanning program to capture and annotate sequences reported in the literature that had not been submitted to any of the sequence databases. (nih.gov)
databases agreed to adopt the NCBI taxonomy as the standard classification for the nucleotide sequence databases. (nih.gov)

On the contrary, significant amount of genomic information are in the domain for a closely related leguminous crop soybean (Glycine max L.). Here, we have reported an in silico analysis for the identification of differentially expressed genes (DEGs) and their signaling pathways due to drought stress. (longdom.org)
36. Differentially expressed genes associated with hepatitis B virus HBx and MHBs protein function in hepatocellular carcinoma. (nih.gov)

Generation II DNA sequencing has been widely heralded as a disruptive technology, generating tens of millions of random short sequences at efficiencies up to 20,000-fold greater than Generation I (Sanger) sequencing. (ddw-online.com)
The bands were cloned and identified as WUPyV by using Sanger sequencing. (cdc.gov)
A DNA sequencing technique that combines the chain termination method developed by Fred Sanger with aspects of the polymerase chain reaction. (bscs.org)

The mouse gene, tsg101, appears to suppress malignant cell growth, and the putative protein encoded by tsg 101 has the sequence characteristics of a transcription factor. (davidson.edu)
Alignment of the putative human and mouse TSG101 proteins (Figure 1B) shows 94% identity between the protein sequences, with 20 amino acid mismatches and one gap. (davidson.edu)
Here, we analyzed the phylogeny, expression and genomic distribution of 69 putative CCEs in the silkworm, Bombyx mori (Lepidoptera: Bombycidae). (biomedcentral.com)
A putative nuclear export sequence is also apparent, yet a similar motif is absent in mammalian IL-36RN. (peptidesolubility.com)

The sequences presented in the cricket Gene Index will provide neurophysiologists with many genetic tools that have been largely absent in this field. (nih.gov)
Due to degeneracy of most three-nucleotide genetic codons, a SNP in thecoding regions may be synonymous (sSNP) if it does not result in change of theprotein sequence or non-synonymous (nsSNP) if it does. (biomedcentral.com)
SpiroESTdb provides an integrated platform for expressed sequence data, expression dynamics, functional genes, genetic markers including single nucleotide polymorphisms and tandem repeats, gene ontology and KEGG pathway information. (biomedcentral.com)
DNA contains only four nucleotides - adenine (A), thymine (T), cytosine (C), and guanine (G) - but this simple genetic alphabet is the starting point for making all of the proteins in the human body, estimated to be as many as one million. (nih.gov)
The genetic code specifies the sequence of amino acids in a protein. (bscs.org)
A complex cross talk between genetic and environmental factors during neurogenesis is responsible for promoting differences of gene and protein expression in schizophrenia, causing abnormal processes during neurodevelopment. (pharmaceuticalintelligence.com)

We have sequenced and annotated two genomic regions located in the Giemsa negative band q22 of human chromosome 7. (nih.gov)
These fragments were cloned, sequenced, and mapped to chromosome band 14q13. (nih.gov)
Centromere protein (CENP) A, a histone H3 variant, is a key epigenetic determinant of chromosome domains known as centromeres. (elifesciences.org)
A particular sequence variation of a gene or a segment of a chromosome. (bscs.org)
Putting sequenced fragments of DNA into their correct order along the chromosome. (bscs.org)
contiguous sequence of DNA created by assembling shorter, overlapping sequenced fragments of a chromosome (whether natural or artificial, as in BACs). (bscs.org)

EST (Expressed Sequence Tags) databases contain the sequences of a large number of cDNA fragments from a variety of tissues and organisms. (rndsystems.com)
Then, the longest 5' and 3' fragments obtained from PCR amplification were joined into the pAMP1 cloning vector and sequenced. (davidson.edu)
A technique used to separate DNA fragments (and proteins) by their size. (bscs.org)
A list or diagram showing an ordered arrangement of cloned overlapping fragments that collectively contain the sequence of an originally continuous DNA. (bscs.org)

For each of the unique sequences, an attempt was made to assign a provisional annotation and to categorize its function using a Gene Ontology-based classification through a sequence-based comparison to known proteins. (nih.gov)

Two genes showed weak similarity to an insulin-like receptor and a neuronal protein with a leucine-rich amino-terminal domain. (nih.gov)
n\nA novel chicken IL-1 family sequence representing IL-36RN (IL-1F5) was initially identified from an expressed sequence tag (EST) library by its similarity to both chicken IL-1 RN and chicken IL-1 beta. (peptidesolubility.com)

The start and stop codons are underlined and the deduced amino acid sequence given below. (medscape.com)
An open reading frame of 1140 nucleotides found in the cDNA sequence was predicted to encode a 380 amino acid protein with a mass of 42.841 kDa. (davidson.edu)
In addition, the human TSG101 amino acid sequence also contains seven possible protein kinase C phosphorylation sites, five possible casein kinase II phosphorylation sites, and three possible N-glycosylation sites that are found in the mouse protein. (davidson.edu)
A DNA (or amino acid) sequence that has remained relatively unchanged throughout evolution. (bscs.org)
There is evidence that the acquisition of insecticide resistance can arise either by mutations in CCE amino acid sequences that change the activity of the esterase or by amplification of CCE genes in this group [ 1 ]. (biomedcentral.com)

The EST database described here is the first step towards gene discovery in Foraminifera and should provide the basis for new insights into the genomic and transcriptomic characteristics of these interesting but poorly understood protists. (unige.ch)
His most recent project involved analysing transcriptomic data, sequenced from patient blood samples, to identify genes that may be important in the progression from mild cognitive impairment to Alzheimer's disease and the role of inflammation in this process. (exeter.ac.uk)

This role for Rpl22 is not a general consequence of mutating non-essential large ribosomal proteins as strains lacking Rpl29 or Rpl39 execute meiosis with nearly wild-type efficiencies. (biomedcentral.com)
The roles that ribosomal proteins (RPs) themselves play in regulating translation initiation are less well understood. (biomedcentral.com)

SNP discovery usually is accomplished through computational alignment of redundantDNA sequences with each other or with a high-quality reference genome wherediscrepant nucleotides can be detected and evaluated. (biomedcentral.com)
Reference Genome == '''Nucleotide view''' When zoomed in, the DNA sequence of the Release 6 (R6) [http://flybase.org/reports/FBrf0227719.html (Hoskins et al, 2015)] genome is shown. (flybase.org)

Chemokines are divided into subfamilies based on conserved aa sequence motifs. (rndsystems.com)
My earlier work at NIH focused on sequence motifs and conserved domains in proteins involved in signal transduction, particularly those that interact with and regulate GTPases. (nih.gov)
As a first step toward predicting function, related sequences were clustered to build motifs that could be searched against protein databases. (umn.edu)
A coiled-coil domain and a proline-rich region, both typical features of the activation domain of transcription factors, are highly conserved (only one mismatch), and the leucine zipper motifs in that coiled-coil domain of each protein are identical. (davidson.edu)
A comparison of EIF2AK2 protein domains in vertebrates indicates that the kinase catalytic domains were evolutionarily more conserved than the nucleic acid-binding motifs. (gse-journal.org)

The aim in this thesis is to identify and analyse splice variants of the adhesion family of G protein-coupled receptors using EST data. (diva-portal.org)
75% of the genes in the data set of 33 sequences were found to have a total of 51 splice variants. (diva-portal.org)
Splice acceptor and donor sequences were found to conform to the GT-AG rule, which recognizes introns beginning with GT and ending with AG. (medscape.com)

Background transcriptome sequencing to create the first in depth expressed series label (EST) dataset for using high-throughput sequencing technology. (bioxorio.com)
We introduced premature stop codons into mScarlet that enabled high-throughput quantification of protein synthesis termination errors in E . coli using fluorescent microscopy. (biorxiv.org)

We have used 454 pyrosequencing to augment the available gene sequence information to enhance functional genomics and marker design for this plant species.Results: Two quarter 454 pyrosequencing runs used two normalized cDNA collections from stems and leaves from drought-stressed sweetpotato clone Tanzania and yielded 524,209 reads, which were assembled together with 22,094 publically available expressed sequence tags into 31,685 sets of overlapping DNA segments and 34,733 unassembled sequences. (edu.pe)

To assess the progression of acute lung injury, the temporal expression of genes and expressed sequence tags was assessed by complementary DNA microarray analysis. (nih.gov)

We used BLAST algorithms to compare unigene sets from Medicago truncatula, Lotus japonicus, and soybean (Glycine max and Glycine soja) to nonlegume unigene sets, to GenBank's nonredundant and EST databases, and to the genomic sequences of rice (Oryza sativa) and Arabidopsis. (umn.edu)
Furthermore, the protein sequence databases ( SWISS-PROT and PIR ) each developed their own taxonomic classifications that were very different from each other and from the nucleotide database taxonomies. (nih.gov)
The resulting hybrid was, at first, a bigger mess than any of the pieces had been, but it gave us a starting point that spanned all of the names in all of the sequence databases. (nih.gov)

This study reveals that the genes expressed due to drought stress and the pathways triggered in soybean were similar with the pigeonpea and hence, it can be extrapolated for further biochemical analysis. (longdom.org)
This is the highest proportion of genes expressed in any part of the body. (nih.gov)
To identify the genes expressed in normal human trabecular meshwork tissue, a tissue critical to the pathogenesis of glaucoma. (molvis.org)

Genomic amplification is observed in many, if not all, types of human malignancy and is one of the mechanisms for the activation of dominant-acting oncogenes in tumorigenesis. (nih.gov)
Using the sequence tagged site-amplification mapping approach, we defined the core-amplified domain by screening 75 normal-tumor paired esophageal samples. (nih.gov)
Increased immunohistochemical nuclear staining of the HNF3alpha protein was detected in all of the tumors containing 14q13 amplification. (nih.gov)

Evolutionary analyses of the genomic sequences of several CCPs in M. truncatula suggest that this family has evolved by local duplications and divergent selection. (umn.edu)
2005) Comparative analyses of six solanaceous transcriptomes reveal a high degree of sequence conservation and species-specific transcripts. (insilicogen.com)
2003) Comparative analyses of potato expressed sequence tag libraries. (insilicogen.com)
By subcellular fractionations, protein interaction analyses, subcellular reconstitutions of protein complexes, colocalization studies in cells and tissues, and by functional analyses in neuronal morphogenesis we demonstrate that both proteins associate and work with each other closely. (jneurosci.org)

Together with the above-mentioned techniques, proteomics have been contributing to understanding the biochemical basis of schizophrenia at the cellular and tissue level through the identification of differentially expressed proteins and consequently their biochemical pathways, mostly in the brain tissue but also in other cells. (pharmaceuticalintelligence.com)
In addition, mass spectrometry-based proteomics have identified and precisely quantified proteins that may serve as biomarker candidates to prognosis, diagnosis, and medication monitoring in peripheral tissue. (pharmaceuticalintelligence.com)

This allowed the identification of tissue-specific genes using enrichment analysis for GO pathways and protein domains. (marbigen.org)
Expressed sequence tag (EST) counts per unigene were used for in silico expression analysis by online IDEG6 web statistical tool. (longdom.org)
Analysis of expressed sequence tags from a naked foraminiferan Reticulomyxa filosa. (unige.ch)
Two-dimensional clustering analysis of the expressed genes for the Δupf1 , Δupf2 and Δupf3 strains yielded several interesting results. (biomedcentral.com)
Although I continue to study the interrelationships of sequence, structure, and function in proteins, I have also been working on information analysis and retrieval problems in genome research. (nih.gov)
S erial Analysis of gene Expression(SAGE), Cap Analysis of gene expression (CAGE), 그리고 Massively Parallel Signature sequencing(MPSS)은 특정 유전자의 발현 양 정보를 얻고자 하는 목표로 수행되는 방법들이다. (insilicogen.com)
Here, we report the sequencing and analysis of the sacred lotus genome, which descends from the most ancient lineage of angiosperms. (biomedcentral.com)
This technology acts as a GPS-like system for mapping the exact location of each of the thousands of individual cells undergoing genomic analysis in a tissue sample. (nih.gov)
Our genomic analysis has provided novel information on the CCEs of the silkworm, which will be of value to understanding the biology, physiology and evolution of insect CCEs. (biomedcentral.com)

On the basis of this gene index, we have identified 1,661 gene-based microsatellite sequences, of which 223 were selected for testing and 195 were successfully amplified in a test panel of 6 hexaploid (I. batatas) and 2 diploid (I. trifida) accessions.Conclusions: The sweetpotato gene index is a useful source for functionally annotated sweetpotato gene sequences that contains three times more gene sequence information for sweetpotato than previous EST assemblies. (edu.pe)
Our efforts to reveal how Cobl is physically and functionally integrated into the cortical actin cytoskeleton showed that all Cobl loss-of-function phenotypes were exactly mirrored by knockdown of the F-actin-binding protein Abp1. (jneurosci.org)
The carboxyl/cholinesterase (CCE) superfamily is comprised of functionally diverse proteins that hydrolyze carboxylic esters to their component alcohols and acids. (biomedcentral.com)

Overall, our findings suggest that the environment influences the accuracy of protein production, which increases protein heterogeneity when the organisms need to adapt to new conditions. (biorxiv.org)
The process of learning about human genetics by comparing human DNA sequences with those from other organisms. (bscs.org)

Western clawed frog and fugu EIF2AK2 gene sequences were detected in the USCS Genome Browser and compared to those of other vertebrate EIF2AK2 genes. (gse-journal.org)

Proteome-wide detection of stop codon miscoding by mass spectrometry revealed that temperature regulates the expression of cryptic sequences generated by stop codon miscoding in E . coli . (biorxiv.org)
proteomic peptides''' Alignment of peptide sequences identified in the developmental proteome of Casas-Vila et al. (flybase.org)

The band was sequenced, and the 5' and 3' ends of the transcript were determined by RACE. (medscape.com)

The non-exonic component of the genome accomplishes control primarily by transcription into a plethora of regulatory RNA types (including smRNA, ncRNA, tRNA, rRNA and antisense transcription) or by containing structural genomic DNA features that influence transcription and replication (both control elements and DNA methylation). (ddw-online.com)

abstract = "The Fabaceae, the third largest family of plants and the source of many crops, has been the target of many genomic studies. (umn.edu)

Presently less genomic resources are available on pigeonpea for drought related studies at molecular level. (longdom.org)
However, surprisingly little is known about the molecular function of genes that are expressed during the course of the parasite life cycle. (biomedcentral.com)
Provided the clinical need for this proteins, a lot of hard work provides been directed toward characterizing and understanding the molecular basis of the conversation of streptokinase with ZD6474 distributor plasminogen. (buyresearchchemicalss.net)

Protein synthesis termination is optimized to have high fidelity, yet it is not perfect. (biorxiv.org)
For instance, infidelity in protein synthesis termination can be adaptive and functional 7 , 9 , 11 - 16 or non-adaptive 17 , and it sometimes leads to fitness decrease 18 . (biorxiv.org)
These tremendous evolutionary implications reveal the need to study the rules that dictate inaccuracy of protein synthesis termination under diverse living conditions. (biorxiv.org)

Ov-FAR-1 and ABA-1A1 may instead behave similarly to extracellular lipid-binding proteins. (wikipedia.org)

Approximately 25,000 protein-coding genes are interspersed throughout the genome as discontinuous sets of exons. (ddw-online.com)

37. Quantitative proteomic signature of liver cancer cells: tissue transglutaminase 2 could be a novel protein candidate of human hepatocellular carcinoma. (nih.gov)

Induction of merogony by FR235222 leads to an increase in expression of merozoite-marker (rhoptry) proteins. (nature.com)
In contrast, expression levels of surfactant proteins (SPs) and Clara cell secretory protein (ie, transcripts that are constitutively expressed in the lung) decreased markedly. (nih.gov)
Direct comparison of gene expression in the hypothalamus of animals from the extremes of the body weight distribution identified a number of differentially expressed transcripts in lean versus obese animals. (medscape.com)
In yeast, NMD depends on the expression of the Upf1, Upf2 (Nmd2) and Upf3 proteins. (biomedcentral.com)
35. Gene expression profiling of human HBV- and/or HCV-associated hepatocellular carcinoma cells using expressed sequence tags. (nih.gov)
이번 연재에서는 Next Generation Sequencing의 두 번째 Application인 Expression study 중에 유전자와 엑손의 발현 및 발현된 유전자의 각종 변이 등을 한 번에 연구할 수 있는 RNA-Seq 분석 에 대해 알아보겠습니다. (insilicogen.com)
The expression pattern of each B. mori CCE was also investigated by a search of an expressed sequence tag (EST) database, and the relationship between phylogeny and expression was analyzed. (biomedcentral.com)
Effector-encoding genes are expressed only during plant infection and show very low expression during other developmental stages. (exeter.ac.uk)
Using this simple screen, we identify Rgs1, a regulator of G-protein signaling (RGS) protein that is necessary for appressorium development, as a novel transcriptional regulator of effector gene expression, which acts prior to plant infection. (exeter.ac.uk)

Alignment of peptide sequences determined by mass spectroscopy, derived from polypeptides isolated from the sequenced strain at various developmental stages. (flybase.org)

Measuring survival time, protein and neutrophil concentrations in BAL fluid, lung wet-to-dry weight ratio, and histology, we found that these responses varied between inbred mouse strains and that susceptibility is heritable. (nih.gov)
Streptokinases secreted by nonhuman isolates of group C streptococci (strains which activated either equine or porcine plasminogen were cloned, sequenced, and expressed in isolates but also these proteins participate in a category of plasminogen activators even more diverse than previously idea. (buyresearchchemicalss.net)

When secreted, these proteins may modify the host's immune response in order to promote longevity of the parasite. (wikipedia.org)
Other helminth proteins promote parasite survival in other ways, particularly since parasites must depend on hosts for the supply of essential nutrients. (wikipedia.org)
Through secretion of a repertoire of proteins on to the parasite surface or translocated into the host cell compartment, the schizont fine tunes host cell signalling pathways to create an infected leukocyte with cancer-like properties 8 . (nature.com)

Transcription and translation errors may generate short-lasting phenotypic variability on a physiological time scale, faster than genomic mutations. (biorxiv.org)
These mutations appear to alter the structure and function of the POLR1C protein, which reduces the amount of functional RNA polymerase I and RNA polymerase III in cells. (medlineplus.gov)

In addition, most NMD substrates were found to be upregulated upon NMD inactivation, but some were downregulated, pointing to the existence of higher-order NMD targets (or additional functions of the Upf proteins in alternative gene-regulation pathways). (biomedcentral.com)

A processed pseudogene of the beacon gene was identified in the human genome after electrophoresis of genomic PCR products revealed two products, one of which corresponded to the size of the cDNA PCR product. (medscape.com)

We report the sequencing of 14,502 EST's from clones derived from a nerve cord cDNA library, and the subsequent construction of a Gene Index from these sequences, from the Hawaiian trigonidiine cricket Laupala kohalensis. (nih.gov)
Clones that cross-reacted with KI polyomavirus VP1 (KI-VP1) were identified by ELISA with glutathione S-transferase-tagged KI-VP1 ( 2 ) and eliminated. (cdc.gov)
In total, 5,760 randomly selected clones were picked and sequenced. (biomedcentral.com)
A human genomic library screened by PCR with TSG101-specific primers yielded two clones that contained the gene. (davidson.edu)

The remainder are larger with additional aa occurring upstream of the protein core or as part of an extended C-terminal segment. (rndsystems.com)
Further, slippery sequences upstream the stop codon that cause the ribosome to slip and thereby lead to stop codon readthrough are often functional 19 , 20 . (biorxiv.org)

These tags were mapped to known unique Unigene clusters. (molvis.org)
Approximately 29% of the tags (orphan tags) did not map to a known Unigene cluster. (molvis.org)
Thirteen percent of the tags mapped to at least 2 Unigene clusters. (molvis.org)

A helminth protein, or helminthic antigen, is a protein derived from a parasitic worm that causes an immune reaction. (wikipedia.org)
Helminth proteins can result in a deregulated response to infection, and are implicated in reduced reactivity to other antigens. (wikipedia.org)
Despite their pathogenic properties, helminth proteins have potential to be co-opted to treat a number of other human diseases. (wikipedia.org)
Helminth proteins modulate the immune response of their hosts, but do not suppress it entirely. (wikipedia.org)
Additionally, the database can also be used to develop protein targets for new drugs to treat helminth infections. (wikipedia.org)
Given the modulatory properties of helminth proteins, it has been suggested that they may be co-opted to successfully treat other human diseases, particularly those associated with auto-immunity disorders. (wikipedia.org)

Chemokines are a superfamily of mostly small, secreted proteins that function in leukocyte trafficking, recruiting, and recirculation. (rndsystems.com)
The Na + -Ca 2+ exchanger (NCX) is an integral membrane protein belonging to the Ca 2+ /cation: antiporter (CaCA) superfamily of protein transporters [ 1 ]. (biomedcentral.com)

Stop codon miscoding events give rise to longer proteins, which may alter the protein's function and thereby generate short-lasting phenotypic variability from a single gene. (biorxiv.org)

Out of 52 DEGs, 4 up-regulated and 30 down-regulated genes showed blast hits against pigeonpea sequences and, their relation to drought response was confirmed by comparing with the previous studies. (longdom.org)
Genomic surveillance uncovers a pandemic clonal lineage of the wheat blast fungus. (exeter.ac.uk)
This underscores the urgent need for genomic surveillance to track and mitigate the spread of wheat blast outside of South America and to guide preemptive wheat breeding for blast resistance. (exeter.ac.uk)
To cause rice blast disease, the filamentous fungus Magnaporthe oryzae secretes a battery of effector proteins into host plant tissue to facilitate infection. (exeter.ac.uk)

Performance was improved by accelerating the loading of central data pages such as the omnibus Gene page, by rationalizing internal data structures and software for greater portability, and by making the Genome Browser highly customizable in how it views and exports genomic subsequences. (nih.gov)
Arbitrarily complex, user-specified queries are now possible through Textpresso (for all available literature) and through WormMart (for most genomic data). (nih.gov)
genomic data [38]. (bioxorio.com)
Comprehensive descriptions of the sparganum content of all sequenced data are available, including summary reports. (biomedcentral.com)
Both systems allow searching of the Taxonomy database for names, and both link to the relevant sequence data. (nih.gov)
He is now looking at how long-read sequence data produced by Oxford Nanopore can be used to study patterns of epigenetic modification in a number of neurodegenerative diseases. (exeter.ac.uk)

A complex has been isolated containing Spc110p, Spc42p, calmodulin and a 35 kDa protein, suggesting that these four proteins interact in the SPB. (sdbonline.org)

All chemokines signal through seven transmembrane domain G-protein coupled receptors. (rndsystems.com)
Examination of messenger RNA purified from those highly enriched PHSCs revealed that c-kit, stem cell factor (SCF), and the receptors for the hematopoietic growth factors interleukin-3 (IL-3) and interleukin-6 (IL-6) were all expressed at high levels. (nih.gov)

The protein is not predicted to have an NH 2 -terminal signal peptide sequence or any transmembrane regions. (medscape.com)
SNPs are most abundant among genomic DNAvariations and ubiquitous in both functional genes and non-coding regions [ 1 ]. (biomedcentral.com)
Repeat region''' Regions of genomic repeats and low complexity DNA sequences (in pink), as computed using RepeatMasker and RepeatRunner [http://{{flybaseorg}}/reports/FBrf0194515.html (Smith, et al. (flybase.org)
To identify the regions of properdin important for function, we have expressed human properdin, and mutant forms each lacking a single TSR, in Chinese hamster ovary cells. (embl.de)

This study aimed at increasing genomic resources for the two species and produced and annotated two sets of 30,000 expressed sequence tags (EST) each from 14 normalized tissue-specific cDNA libraries from sea bream and sea bass. (marbigen.org)
Proteins form the internal machinery within brain cells and the connective tissue between brain cells. (nih.gov)

Our goal is to locate all of these transcribed coding sequences, starting with a comprehensive set of cDNAs, called expressed sequence tags, and map them back with high resolution onto the chromosomes. (nih.gov)

Anatomy11

Organisms25

Diseases3

Chemicals and Drugs21

Analytical, Diagnostic and Therapeutic Techniques and Equipment28

Phenomena and Processes82

Disciplines and Occupations4

Technology, Industry, Agriculture5

Information Science14

Health Care3

Predicted genes1

ESTs16

GenBank6

MRNA11

Needed genomic resources1

Genomes4

MRNAs6

Availability of genomic2

Biological6

Homology5

Characterization2

Locus3

Nucleotide sequences1

NCBI4

Differentially expressed genes2

Sanger3

Putative4

Genetic6

Chromosome6

Fragments4

Known proteins1

Similarity2

Amino acid5

Transcriptomic2

Ribosomal proteins2

Reference genome2

Motifs5

Splice3

High-throughput2

Genomics1

Complementary1

Databases3

Genes expressed3

Amplification3

Analyses4

Proteomics2

Analysis9

Functionally3

Organisms2

Gene sequences1

Proteome2

Transcript1

RRNA1

Abstract1

Molecular3

Synthesis3

Extracellular1

Exons1

Proteomic1

Expression9

Alignment1

Strains2

Parasite3

Mutations2

Pathways1

Electrophoresis1

Clones4

Upstream2

Unigene3

Helminth6

Superfamily2

Phenotypic1

BLAST4

Data6

Interact1

Receptors2

Regions4

Tissue2

CDNAs1