• Site-specific iterations of biological clues or cumulative issues Compared at other models in the F2 Mechanisms of Inorganic and Organometallic thrive for using the solution of the processes of these cells and network or cycle proteins and Modelling interactions about sufficient capsids and genes of PPT sites. (scoutconnection.com)
  • 19 In 1987, Plant Genetic Systems (Ghent, Belgium), founded by Marc Van Montagu and Jeff Schell, was the first company to develop genetically engineered (tobacco) plants with insect tolerance by expressing genes encoding for insecticidal proteins from Bacillus thuringiensis (Bt). (truthwiki.org)
  • Although all plasmids basically function in similar ways, what makes targeting plasmids difficult is that the genes and proteins they need for multiplication and stable inheritance are highly diverse making it unlikely to find a single compound that will block them all. (resistancecontrol.info)
  • Horizontal gene transfer is the primary mechanism for the spread of antibiotic resistance in bacteria, and plays an important role in the evolution of bacteria that can degrade novel compounds such as human-created pesticides and in the evolution, maintenance, and transmission of virulence. (wikipedia.org)
  • Genes responsible for antibiotic resistance in one species of bacteria can be transferred to another species of bacteria through various mechanisms of HGT such as transformation, transduction and conjugation, subsequently arming the antibiotic resistant genes' recipient against antibiotics. (wikipedia.org)
  • The rapid spread of antibiotic resistance genes in this manner is becoming a challenge to manage in the field of medicine. (wikipedia.org)
  • Inter-bacterial gene transfer was first described in Japan in a 1959 publication that demonstrated the transfer of antibiotic resistance between different species of bacteria. (wikipedia.org)
  • We anticipate that, over the next decade, CRISPR and other gene editing techniques will be used to rapidly develop elite strawberry varieties with improved disease resistance, fruit quality, and other valuable attributes. (ufl.edu)
  • For example, instead of breeding for many years to move a disease resistance gene from a wild strawberry into a modern strawberry, gene editing allows a direct introduction of the genetic information. (ufl.edu)
  • The UF/IFAS strawberry breeding program has identified several important gene regions controlling disease resistance traits that are directly relevant to Florida growers. (ufl.edu)
  • Each of Recombinetics' calves possessed two antibiotic resistance genes, along with other segments of superfluous bacterial DNA. (greenmedinfo.com)
  • Agrobacterium cells with a genetically-modified plasmid, containing a gene for the desired trait and a marker gene, usually conferring antibiotic resistance, are incubated with protoplasts or small pieces of plant tissue. (jrank.org)
  • The gene encoding Bt, as well as genes conferring resistance to viral diseases, have been introduced into plants by this method. (jrank.org)
  • GM corn and cotton contain bacterial genes that kill insects and confer herbicide-resistance on the crops. (jrank.org)
  • GM squash contains viral genes that confer resistance to viruses. (jrank.org)
  • Moin M., Saha A., Bakshi A., Divya D., Madhav M.S., Kirti P.B. (2021) Study on Transcriptional Responses and Identification of Ribosomal Protein Genes for Potential Resistance against Brown Planthopper and Gall Midge Pests in Rice. (abfindia.org)
  • Development of transgenic cotton (Narasimha) using triple gene Cry2Ab-Cry1F-Cry1Ac construct conferring resistance to lepidopteran pest. (abfindia.org)
  • Plasmids play a key role in the ecology and evolution of bacterial populations as they frequently carry genes conferring traits such as antibiotic resistance and pathogenesis. (platt-lab.com)
  • Bacterial resistance against antibiotic treatment has become a major threat to public health. (springeropen.com)
  • 7 It is now known that there are several natural mechanisms for flow of genes, or (horizontal gene transfer), and that these occur in nature on a large scale - for example, it is a major mechanism for antibiotic resistance in pathogenic bacteria, and it occurs between plant species. (truthwiki.org)
  • 14 Since then important traits have been introduced into wheat, including dwarfing genes and rust resistance. (truthwiki.org)
  • Selective culture enrichment and sequencing of feces to enhance detection of antimicrobial resistance genes in third-generation cephalosporin resistant Enterobacteriaceae. (cdc.gov)
  • US immigration is associated with rapid and persistent acquisition of antibiotic resistance genes in the gut. (cdc.gov)
  • Plasmids are recognized as one of the major contributors to the rapid worldwide spread of resistance genes. (resistancecontrol.info)
  • One of the factors that heavily contributes to the problem is how rapidly novel and existing resistance determinants move among bacterial hosts. (resistancecontrol.info)
  • Many globally successful strains of pathogens have been able to become widely disseminated as a result of resistance genes acquired on mobile genetic elements (MGEs) (1) . (resistancecontrol.info)
  • Amongst the favourable traits carried on plasmids, genes conferring antibiotic resistance are of particular concern in the spread of drug-resistant infections (6) . (resistancecontrol.info)
  • Genes such as NDM-1 conferring resistance to β -lactam antibiotics, and other essential antibiotics, have been observed on dominant plasmid types that are known to have been in circulation for over 50 years. (resistancecontrol.info)
  • GMO technology offers, for example, the possibility of engineering resistance to the corn rootworm by introducing a bacterial transgene into corn or the ability to test a gene from any source in a plant. (umn.edu)
  • The selected gene can be inserted in any one of these resistance gene. (eagetutor.com)
  • It has been suggested that lateral gene transfer to humans from bacteria may play a role in cancer. (wikipedia.org)
  • Transformation is often used in laboratories to insert novel genes into bacteria for experiments or for industrial or medical applications. (wikipedia.org)
  • Scientists have discovered genes from bacteria in sweet potatoes around the world. (tipsbisnisseo.net)
  • Scientists at the International Potato Center in Lima, Peru, discovered genes from the bacteria in 291 potato varieties, including those grown in the United States, Indonesia, China, parts of South America and Africa. (tipsbisnisseo.net)
  • Scientists take some plant cells and mix them with a special bacteria called Agrobacterium. (tipsbisnisseo.net)
  • this gene is a very useful reporter in other gram-negative bacteria because its activity can be measured by a very sensitive assay ($105-fold more sensitive than conventional reporters such as b-galactosidase) and its expression can be quantified by very simple methods, such as a droplet-freezing assay (6, 22, 9). (moam.info)
  • However, it is likely that several horizontal gene transfers (HGT) also occurred in eukaryotic microorganisms and, even more surprisingly, in bacteria. (biomedcentral.com)
  • The current widespread occurrence of multidrug-resistant (MDR) bacteria has become a major burden on national health services, with death rates due to bacterial infections on the rise. (resistancecontrol.info)
  • In addition, many plasmids can transfer from one bacterium to another, the most sophisticated mechanism being by conjugation in which the plasmid carries genes that can create a bridge between bacteria through which a copy of the plasmid can move (Fig 1a). (resistancecontrol.info)
  • Plasmids can also pick up a variable cargo of other genes that can help their host bacterium grow or survive in different environments - the plasmid spreads them between bacteria and if a survival advantage is gained due to the carried genes on the plasmid there will be positive selection for its carriage. (resistancecontrol.info)
  • As discussed in the chapter on DNA, the genetic code is the sequence of bases along a strand of DNA, and is universal among all living organisms, enabling the transfer of a gene from a bacteria, virus, or any organism to a plant. (umn.edu)
  • Others see the "unnatural" movement of a gene from a bacteria to a plant as a process that we should not use. (umn.edu)
  • Understanding the presence of plasmid vectors requires an appreciation of the basics of gene-editing, which, confusingly, are considerably distinct from what the word 'editing' means in ordinary English. (greenmedinfo.com)
  • Plasmid vectors have been developed, mutants can be isolated by various mutagenic treatments, and various genes have been cloned in heterologous hosts such as Escherichia coli (17). (moam.info)
  • The development of VIGS vectors for P. sativum , G. max and P. vulgaris has paved the way for functional analysis of plant symbiosis genes in crop legumes and the transfer of knowledge from model legumes to crop plants. (biomedcentral.com)
  • Two plasmid vectors that have been extensively used in genetics are pBR322 and pUC18. (eagetutor.com)
  • With gene editing, the final product can match that obtained by conventional plant breeding, but in a much shorter timeframe. (ufl.edu)
  • Alterations in the expression of genes involved in gibberellin (GA) biosynthesis and inactivation results in increased and decreased biomass accumulation, respectively, which is positively correlated with the photosynthetic rates at the whole plant level [ 8 ]. (biomedcentral.com)
  • Most recently, the potential for plant breeding has advanced significantly, with the advent of methods for the incorporation of genes from other organisms into plants via recombinant DNA-techniques. (jrank.org)
  • Advantage has been taken of the natural ability of Agrobacterium to transfer plasmid DNA into the nuclei of susceptible plant cells. (jrank.org)
  • Two methods have been developed for direct gene transfer into plant cells-electroporation and biolistics. (jrank.org)
  • Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan. (cornous.com)
  • CRISPR/Cas9-mediated targeted mutagenesis of GmSPL9 genes alters plant architecture in soybean. (cornous.com)
  • Increased understanding of plant genetics and the development of powerful and easier-to-use gene editing tools over the past century have revolutionized humankind's ability to deliver precise genotypes in crops. (frontiersin.org)
  • Plant transformation techniques are well developed for making transgenic varieties in certain crops and model organisms, yet reagent delivery and plant regeneration remain key bottlenecks to applying the technology of gene editing to most crops. (frontiersin.org)
  • This review highlights diverse strategies to deliver CRISPR/Cas gene editing reagents to regenerable plant cells and to recover edited plants without unwanted integration of transgenes. (frontiersin.org)
  • In many crops, genome engineering techniques have enabled researchers and breeders to take advantage of a vast knowledge base of plant physiology, pathology, and genetics. (frontiersin.org)
  • Research shows that a bacterium carried the gene into plant ancestors long before humans started cooking the delicious aedes. (tipsbisnisseo.net)
  • We think the bacterial gene helps the plant produce two hormones that change the root and make it eat," Kreuze told Goats and Soda. (tipsbisnisseo.net)
  • Every cell in this plant contains the bacteria's genes. (tipsbisnisseo.net)
  • Inserting a fragment of a plant gene into a virus vector will result in a recombinant virus that triggers degradation of both the virus transcript and of homologous endogenous plant RNA sequences. (biomedcentral.com)
  • Virologists, those devoting their lives to the study of virus biology, knew that the virus' genome had a gene encoding a coat protein that surrounded the virus' genetic material and that was essential to its infection of plant cells. (umn.edu)
  • This opposite orientation of the viral gene, now acting as plant gene, effectively shuts down viral replication before it can cause disease, making the plant resistant. (umn.edu)
  • To introduce the engineered viral gene into the plant's chromosome without crossing, horticulturist John Sanford invented a means of introducing genes into plant cells by literally shooting the gene into the nucleus of a cell. (umn.edu)
  • However, in the early 1970s, Stanley Falkow, Stanley Cohen, Herbert Boyer, Donald Helinski, Charles Brinton and several others developed the concept of using plasmids as tools for gene cloning. (resistancecontrol.info)
  • Plasmids are typically circular (but also less commonly linear) double-stranded DNA molecules that are able to independently control their multiplication and stable inheritance from generation to generation in their bacterial h osts (2) . (resistancecontrol.info)
  • Automatically, plasmids replicate inside the bacterial chromosome. (eagetutor.com)
  • These plasmids can have a gene of 10 kb size too. (eagetutor.com)
  • The naturally occurring plasmids have been classified by the characteristics coded by the plasmid genes. (eagetutor.com)
  • Complete genome sequencing of Agrobacterium sp. (uni-bielefeld.de)
  • Using this new technique, we can cut a strawberry's genome at a desired location so that existing genes can be removed or added. (ufl.edu)
  • But FDA's findings are potentially trivial besides another recent discovery about gene-editing: that foreign DNA from surprising sources can routinely find its way into the genome of edited animals. (greenmedinfo.com)
  • Dutta M., Saha A., Moin M. and Kirti P.B. (2021) Genome-Wide Identification, Transcript Profiling and Bioinformatic Analyses of GRAS Transcription Factor Genes in Rice. (abfindia.org)
  • These NBPTs proved beneficial in the improvement of soybean through precision genome engineering and gene functional characterization. (cornous.com)
  • The gradual conversion from endosymbiont to organelle during the course of evolution has clearly been accompanied by a dramatic reduction in genome size as the chloroplasts lost most of their genes to the nucleus. (frontiersin.org)
  • Here we performed a genome-wide CRISPR-Cas9 screening to identify genes participating in tumor cell dissemination and revealed that PTGES3 acts as an invasion suppressor in ovarian cancer. (bvsalud.org)
  • Horizontal gene transfer (HGT) or lateral gene transfer (LGT) is the movement of genetic material between organisms other than by the ("vertical") transmission of DNA from parent to offspring (reproduction). (wikipedia.org)
  • There are several mechanisms for horizontal gene transfer: Transformation, the genetic alteration of a cell resulting from the introduction, uptake and expression of foreign genetic material (DNA or RNA). (wikipedia.org)
  • In RIT's biotechnology and molecular biology degree you'll improve human health by harnessing technology advancements and biomolecular processes to research and develop technologies in genetics, agriculture, pharmaceuticals and vaccine development, environment and energy, forensic science, genetic counseling, and more. (rit.edu)
  • By using CRISPR technology, these genes or gene variants can be moved into desirable genetic backgrounds that can be further moved via conventional crossing in later generations. (ufl.edu)
  • We study the ecological and genetic factors influencing the evolution of facultative bacterial pathogens as well as the disease ecology consequences of these dynamics. (platt-lab.com)
  • Genetic engineering methods i.e. gene silencing and transgenesis have reduced many risks and helped to increase soybean resilience. (cornous.com)
  • However, several other routine methods and tools for molecular genetic investigations, including the use of reporter genes for analysis of gene transcription and promoter function, have not yet been developed in Z. mobilis. (moam.info)
  • These techniques are based upon the preparation of hybrid genes, i. e. genes comprising genetic material encoding the polypeptide of interest linked to genetic material additional to the gene of interest. (justia.com)
  • citation needed] Transduction, the process in which bacterial DNA is moved from one bacterium to another by a virus (a bacteriophage, or phage). (wikipedia.org)
  • The PEP enzyme recognizes the -10 and -35 cis -elements, similar to those found in bacterial promoters whereas the NEP enzyme recognizes the YRTA-motif, which can also be found upstream of several genes with PEP promoters indicating that these genes can be transcribed by both polymerases ( Pfannschmidt and Liere, 2005 ). (frontiersin.org)
  • It is found in bacterial cells and some other type of cells. (eagetutor.com)
  • Crown gall is one of the most destructive bacterial diseases of plants. (ac.ir)
  • Based on policy discussions, it is possible that the first-generation plants containing the edited genes will not require extensive regulation, and these tools will be extremely valuable in the long-term efforts of every strawberry breeding program. (ufl.edu)
  • Here, we generated transgenic tomato plants by overexpressing Dwarf , a BR biosynthetic gene that encodes the CYP85A1, and compared the photosynthetic capacity with the BR biosynthetic mutant d im and wild type. (biomedcentral.com)
  • The Panax ginseng PgTIP1 gene confers enhanced salt and drought tolerance to transgenic soybean plants by maintaining homeostasis of water, salt ions and ROS. (cornous.com)
  • There is lack of consideration for a large segment of the population that has ethical or religious beliefs that classify genetically engineered plants carrying animal or human genes as being totally unacceptable as foods. (jesus-is-savior.com)
  • The ubiquitous presence of many of these species in plants and other natural habitats makes bacterial ice nucleation a common phenomenon in nature and a major factor contributing to frost damage to agronomic crops (reference and references therein). (moam.info)
  • Virus-induced gene silencing (VIGS) is an alternative reverse genetics tool for silencing of genes in some plants, which are difficult to transform. (biomedcentral.com)
  • Virus-induced gene silencing (VIGS) exploits a natural defence mechanism in plants against virus infection [ 1 ]. (biomedcentral.com)
  • Thus, allows the recovery of new bacterial species from heavily virulent capsular strains were phagocytosed by and repli- contaminated samples and might be a valuable approach cated in Acanthamoeba castellanii , leading to amoebal for the recovery of as-yet unrecognized emerging death, while the nonvirulent acapsular strains were killed pathogens from clinical specimens. (cdc.gov)
  • The expression of the ice nucleation gene inaZ from Pseudomonas syringae in Zymomonas mobilis strains under the control of three different promoters was investigated to establish the utility of the gene as a reporter and examine the possible use of the organism as a source of ice nuclei for biotechnological applications. (moam.info)
  • This rapid spread is in part due to the extensive movement of people, goods and animals for example, all of which allow for the interaction between different bacterial strains and their MGEs, resulting in a globalized gene pool (2) . (resistancecontrol.info)
  • The pea early-browning virus (PEBV) has been developed as a VIGS vector and used in pea for functional analysis of several genes. (biomedcentral.com)
  • Recent technology has been developed to precisely engineer genes for traits of interest. (ufl.edu)
  • CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is one form of gene editing that can be used to precisely modify a gene of interest without otherwise compromising the favorable traits of an elite variety (Rani et al. (ufl.edu)
  • However, just as each cultivar has different traits and qualities, they also behave differently with respect to introduction of new genes. (ufl.edu)
  • Early indications suggest that gene editing should be regulated like conventionally bred crops, as the final product can simply match what may be done by conventional breeding. (ufl.edu)
  • In non-GMO crops, all genes originate from sexual reproduction. (umn.edu)
  • Unlike rose and apple breeding, in which hobbyists can breed new crops in their gardens, GMO technology integrates knowledge from genetics, molecular biology, and tissue culture to produce a new GMO crop. (umn.edu)
  • Based on phylogenetic analyses, the isolate could be assigned to the genus Agrobacterium biovar I and represents a genomic species G1 strain within this biovariety. (uni-bielefeld.de)
  • This method has proven valuable for maize, rice, and sugar cane, species that are outside the host range for vector transfer by Agrobacterium . (jrank.org)
  • 1] Animal experimentation is going on all over the world the genes from chicken, cows, rats and humans have been engineered into commercial fish species in order to increase the growth rates- GM salmon, for instance, have been engineered to reach six times their natural size. (jesus-is-savior.com)
  • Gautam R, Meena R.K., Woch N, Kirti P.B. (2020) Ectopic expression of BrALDH7B2 gene encoding an antiquitin from Brassica rapa confers tolerance to abiotic stresses and improves photosynthetic performance under salt stress in tobacco. (abfindia.org)
  • Some examples include delivering DNA-free gene editing reagents such as ribonucleoproteins or mRNA, relying on reagent expression from non-integrated DNA, using novel delivery mechanisms such as viruses or nanoparticles, using unconventional selection methods to avoid integration of transgenes, and/or avoiding tissue culture altogether. (frontiersin.org)
  • We have investigated the expression of an ice nucleation gene, inaZ, originally cloned from Pseudomonas syringae (5), in Z. mobilis for two reasons. (moam.info)
  • Production of the fusion polypeptide involves the introduction of the hybrid gene into a biological host cell system, for example yeast cells, which permits the expression and accumulation of the fusion polypeptide. (justia.com)
  • Horizontal gene transfer is recognized as a pervasive evolutionary process that distributes genes between divergent prokaryotic lineages and can also involve eukaryotes. (wikipedia.org)
  • As Jian, Rivera and Lake (1999) put it: "Increasingly, studies of genes and genomes are indicating that considerable horizontal transfer has occurred between prokaryotes" (see also Lake and Rivera, 2007). (wikipedia.org)
  • Some Lepidoptera (e.g. monarch butterflies and silkworms) have been genetically modified by horizontal gene transfer from the wasp bracovirus. (wikipedia.org)
  • Aaron Richardson and Jeffrey D. Palmer state: "Horizontal gene transfer (HGT) has played a major role in bacterial evolution and is fairly common in certain unicellular eukaryotes. (wikipedia.org)
  • The conjugative systems that they often encode allow for their horizontal transmission to other bacterial cells. (platt-lab.com)
  • Joshua Lederberg first coined the term "plasmid" in 1952 (3) , and since their discovery as "extra-chromosomal hereditary determinants", studies have highlighted their role in horizontal gene transfer (HGT) and usefulness as tools in molecular biology. (resistancecontrol.info)
  • They imply, at the very least, the need for strong measures to prevent contamination by stray DNA, along with thorough scrutiny of gene-edited cells and gene-edited organisms. (greenmedinfo.com)
  • The modes of bacterial cell division vary, but the most common one is binary fission (Stahl 2019) , and its mechanism, especially in rod-shaped model organisms Escherichia coli and Bacillus subtilis , has been studied extensively. (hideyuki-ts.com)
  • Potatoes carry the Bt gene to kill the Colorado potato beetle and a viral gene that protects the potato from a virus spread by aphids . (jrank.org)
  • The viral gene is engineered to be in the opposite orientation from its orientation in the virus. (umn.edu)
  • The researchers used RNA-seq to show that depletion of PBP1, a bifunctional penicillin-binding protein, results in a "downregulation in transcription of flagellum-dependent motility genes and an upregulation in transcription of type VI secretion and succinoglycan biosynthesis genes, a hallmark of the ChvG-ChvI regulon. (missouri.edu)
  • It was Besides referred that the suitable types of pyrimidines of rodents at such and bulk Advances( PPT as the protein of chloride, rice, the receptor of copy after coding the vector) mRNA gene analysis combining the polymorphism authors at older promoters( 8, 10, 11). (scoutconnection.com)
  • This approach is known as 'CRISPR' gene editing. (ufl.edu)
  • What is CRISPR gene editing? (ufl.edu)
  • Does CRISPR gene editing = transgenic technology? (ufl.edu)
  • We published another article about CRISPR gene editing in crop improvement ( https://edis.ifas.ufl.edu/publication/HS1334 ). (ufl.edu)
  • These methods are advancing rapidly and already enabling crop scientists to make use of the precision of CRISPR gene editing tools. (frontiersin.org)
  • A bacterial strain producing two antimicrobial peptides was isolated from a rhizosphere soil sample and identified as Bacillus subtilis based on both phenotypic and 16S rRNA gene sequence phylogenetic analysis. (springeropen.com)
  • We need to confirm this, but at the moment we cannot find potatoes without these genes. (tipsbisnisseo.net)
  • When our ancestors first started domesticating potatoes, Krezue said, they noticed swollen roots and selected seeds that carried foreign genes. (tipsbisnisseo.net)
  • 1. Yeast integrative plasmid (Yip) - Used to study the toxicity of a gene. (eagetutor.com)
  • In the mid-1980s, Syvanen postulated that biologically significant lateral gene transfer has existed since the beginning of life on Earth and has been involved in shaping all of evolutionary history. (wikipedia.org)
  • 2005) observe, "additional evidence suggests that gene transfer might also be an important evolutionary mechanism in protist evolution. (wikipedia.org)
  • It also highlights the importance of HGT in the evolutionary history of CHS and describes bacterial chs genes for the first time. (biomedcentral.com)
  • a plastid-encoded bacterial-type RNA polymerase (PEP) and a nuclear-encoded phage-type RNA polymerase (NEP), which recognize distinct types of promoters. (frontiersin.org)
  • Dutta M., Moin M., Saha A., Dutta D., Bakshi A., Kirti P.B. (2021) Gain of function mutagenesis through activation tagging identifies XPB2 and SEN1 helicase genes as potential targets for drought stress tolerance in rice. (abfindia.org)
  • For most commercial applications gene-editing's appeal is simplicity and precision: it alters genomes at precise sites and without inserting foreign DNA. (greenmedinfo.com)
  • Typical protocols to make gene edited (GE) varieties also use transgenes, even though these may be undesirable in the final crop product. (frontiersin.org)
  • 1988) Gene 67: 21-30), glutathione-S-transferase (Johnson (1989) Nature 338: 585-587), ubiquitin (Miller et al. (justia.com)
  • Epidemiology of Bacterial Meningitis in the Nine Years Since Meningococcal Serogroup A Conjugate Vaccine Introduction, Niger, 2010-2018. (cdc.gov)
  • Gene https://doi.org/10.1016/j.gene.2021.145670. (abfindia.org)
  • Gene editing tools are being applied in basic research to close the knowledge gap, but the requirements for gene editing techniques for agriculture are different than those for research. (frontiersin.org)
  • The requirements for such a protocol are to establish virus infection and generation of siRNA molecules for induction of gene silencing in the pea roots before they become AMF colonized. (biomedcentral.com)
  • We are working to understand the adaptive significance of this gene regulation. (platt-lab.com)
  • The silencing potential of the mycorrhiza-PEBV-VIGS protocol was evaluated using two target genes PsSym19 and PsPT4 , with known mutant phenotypes. (biomedcentral.com)
  • He regulates more than 160 E22The methods( in both baseline and same numerous genes of methods, effects and prokaryotes, at free index zu das christlich koptische agypten in arabischer zeit tavo b 41 1 6 tubinger atlas des vorderen orients vigorously fully as to-date fuse) and more than 700 Basic measurements. (naturheilpraxis-gisbert-fussek.de)
  • 310 "A high efficiency method for site-directed mutagen -esis with any plasmid":Gene;1989 (Dec 7);84(1);p153-7.Average yield of mutants was 60% with simple and rapid techniques. (globalchange.com)
  • The prin2.2 and csp41b-2 single mutants displayed pale phenotypes, abnormal chloroplasts with reduced transcript levels of photosynthesis genes and defects in embryo development. (frontiersin.org)
  • The protocol produced reproducible silencing of both symbiosis genes at levels which were sufficient to produce symbiotic phenotypes. (biomedcentral.com)