Genetically cell proliferation. Medical search. Frequent questions

To identify potential therapeutic targets, our lab screens the genes and microRNAs in these signatures for involvement in proliferation, differentiation, and survival of sarcoma cell lines. (mskcc.org)
Consistent with a central role of Wnt signaling in regulation of gene expression, inhibition of PORCN in RSPO3-translocated cancers causes a marked remodeling of the transcriptome, with loss of cell cycle, stem cell and proliferation genes, and an increase in differentiation markers. (nih.gov)
Inhibition of Wnt signaling by PORCN inhibition holds promise as differentiation therapy in genetically defined human cancers. (nih.gov)
We show that loss of Erk1/2 in intestinal epithelial cells results in defects in nutrient absorption, epithelial cell migration, and secretory cell differentiation," the authors wrote. (genengnews.com)
Sylvain Meloche and his team study the signalling mechanisms that control cell division, the differentiation and survival of normal and cancer cells. (iric.ca)
The laboratory of Professor Sylvain Meloche, Director of the Signalling and Cell Growth Research Unit, collaborated with the laboratory of Professor Jean-Claude Labbé, Director of the Cell Division and Differentiation Research Unit, both at IRIC, to identify ERK3 targets and better understand its cellular functions. (iric.ca)
The aim of the present study was to functionalize Ti scaffolds with genetically modified elastin-like recombinamers (ELRs), synthetic polymeric proteins containing the elastin epitopes responsible for their mechanical properties and for promoting mesenchymal stem cell (MSC) recruitment, proliferation, and differentiation to ultimately increase scaffold osseointegration. (ibecbarcelona.eu)
Cell adhesion, proliferation, and colonization were enhanced on those scaffolds functionalized with RGD-ELR, while differentiation was promoted on those with SNA15-ELR. (ibecbarcelona.eu)
The combination of both RGD and SNA15 into the same ELR stimulated cell adhesion, proliferation, and differentiation, although at lower levels than those for every single moiety. (ibecbarcelona.eu)
Further investigation on the amount and distribution of RGD and SNA15 moieties in ELRs could improve cell adhesion, proliferation, and differentiation compared to the present study. (ibecbarcelona.eu)
Our research focuses on developmental pathways that regulate hematopoietic cell growth and differentiation and are disrupted in the course of neoplastic transformation, particularly in leukemias and lymphomas. (stanford.edu)
3) that were divided into two major clusters corresponding to the main phases observed during the preadipocyte culture: proliferation and differentiation. (biomedcentral.com)
Proliferation cluster comprised 1028 genes up-regulated from days 3 to 8 of culture meanwhile the differentiation cluster was characterized by 2140 induced genes from days 15 to 21. (biomedcentral.com)
Overall, our study demonstrates the coordinated expression of functionally related genes during proliferation and differentiation of rainbow trout adipocyte cells. (biomedcentral.com)
Therefore, growth of adipose tissue includes the hypertrophy of already existing adipocytes and the proliferation and differentiation of new ones from MSCs. (biomedcentral.com)
We are particularly interested in the genetic control of differentiation and cell proliferation that pertains to stem cells, progenitors and specialized cells. (umich.edu)
Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)
Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)

Following a single in vivo lentiviral transduction, genetically tagged AMs persisted in lung alveoli and expressed transferred genes for the lifetime of the adult mouse. (jci.org)

However, intestinal epithelial cell proliferation is not impeded, implying compensatory mechanisms. (genengnews.com)
Transgenic zebrafish expressing either wild type or a phosphorylation-resistant form of CagA exhibited significantly increased rates of intestinal epithelial cell proliferation and showed significant upregulation of the Wnt target genes cyclinD1, axin2 , and the zebrafish c-myc ortholog myca . (zfin.org)

To determine the effects of the presence of valproic acid (VA), Wharton's jelly mesenchymal stem cells (MSC) are seeded to the two scaffolds. (asme.org)
In order to understand the development of adiposity, it is crucial to identify the factors and mechanisms that regulate the recruitment of mesenchymal stem cells (MSCs) of the vascular stromal fraction of the adipose tissue and its transformation into lipid-filled adipocytes. (biomedcentral.com)

A) Lymphomas found in Smurf2-deficient mice have the characteristics of diffuse large B-cell lymphoma (DLBCL) and enhanced cell proliferation. (umassmed.edu)
Tazemetostat is a small molecule inhibitor of EZH2 being developed by Epizyme, Inc for the treatment of patients with Non-Hodgkin Lymphoma, including germinal center and non-germinal center diffuse large B-cell lymphoma and follicular lymphoma, as well as genetically-defined solid tumors. (shu.edu)

Our approach starts with purifying the cell type we want and then manipulating it to give those cell types characteristics of progenitor cells, which can grow rapidly but produce only a few cell types. (eurekalert.org)
Though gene transfer-enhanced endothelial progenitor cells (GTE-EPCs) present effectiveness in MI remedy in small animal fashions, no scientific trials utilizing GTE-EPCs have been documented. (inacj.com)
However, the progenitor cells consist of T cells isolated from resected tumor tissue. (msdmanuals.com)
To varying degrees, these fates also extend to the Such state stability is required in stem and progenitor cells to immediate progeny of stem cells, known as progenitor or support self-renewal and maintenance of the uncommitted transit-amplifying cells. (lu.se)

This technology provides a means of converting a patient's own vascular cells, known as endothelial cells, directly into blood stem cells. (businesswire.com)
The endothelial cells are acquired from a biopsied piece of skin and are then "educated" on a bed of VeraVec TM cells (proprietary to Angiocrine Bioscience) to form multipotent blood cells that are capable of producing red cells that carry oxygen, white cells that provide immunity, and platelets to prevent bleeding. (businesswire.com)
Angiocrine's VeraVec TM technology platform is based on endothelial cells that have been genetically modified such that they can be rapidly and durably expanded in culture. (businesswire.com)
Whether or not co-culture of lung carcinoma with PLTs improves the perform of human umbilical vein endothelial cells (HUVECs) requires additional investigation. (inacj.com)
Taken collectively, the outcomes of the current research recommend that tumor cells interacting with PLTs might play an essential position in tumor angiogenesis by affecting or mediating modifications within the properties of vascular endothelial cells (VECs). (inacj.com)
In addition, a recent report [ 15 ] revealed that SSc autoantibodies bind to CMV late protein UL94 and induce apoptosis in endothelial cells therefore implicating molecular mimicry as a potential mechanism accounting for the link between SSc and CMV. (biomedcentral.com)

Lowering ROS levels, using pharmacological or genetic approaches, reduces the level of cell proliferation and impairs tail regeneration. (nih.gov)
Complete morphologic, immunologic, and genetic examination of the leukemic cells is necessary to establish the diagnosis of ALL. (medscape.com)
In addition to these basic issues concerning leukemia pathogenesis, we are devising new diagnostic procedures for detecting and monitoring leukemia patients based on molecular genetic abnormalities in the malignant cells. (stanford.edu)
Multiple sclerosis and T1D have distinct target organs, genetic risk factors, onset ages, and female to male ratios, but target organ-specific T cells as initiators unite these diseases. (frontiersin.org)
The team was led by Dr. Shahin Rafii , director of the Ansary Stem Cell Institute, professor of medicine, genetic medicine and reproductive medicine, and a founder of Angiocrine Bioscience. (businesswire.com)
This offers one of the most challenging paradoxes in cancer biology: what is required of a cell to not only survive massive genetic damage but also outcompete neighboring cells and present as a malignant tumor? (lu.se)
Suggesting that this genetic association is due to pre-existing T-cell immunity, we show that T cells from pre-pandemic individuals carrying HLA-B*15:01 were reactive to the immunodominant SARS-CoV-2 S-derived peptide NQKLIANQF, and 100% of the reactive cells displayed memory phenotype. (cdc.gov)

Bacterial growth is proliferation of bacterium into two daughter cells, in a process called binary fission. (wikipedia.org)
the basic means requires bacterial enumeration (cell counting) by direct and individual (microscopic, flow cytometry), direct and bulk (biomass), indirect and individual (colony counting), or indirect and bulk (most probable number, turbidity, nutrient uptake) methods. (wikipedia.org)
The researchers then used gene editing to create sub-micrometer gas vesicles in bacterial cells, enhancing their acoustic sensitivity. (sciencemission.com)
After the two parts are joined, the plasmid with the DNA insert is inserted into host bacterial or mammalian cells. (thehealthboard.com)

Hence, we established a two-phase expansion protocol with IL-15 to induce an early NK cell expansion, followed by short exposure to IL-21 that boosted the cytotoxic activity of NK cells against RMS cells. (frontiersin.org)
Donor T cells may also induce GvHD due to recognition of allo-antigens on non-malignant tissues of the patient. (haematologica.org)
We further utilized this model to demonstrate that oncogenic cooperation between CagA and a loss-of-function allele of p53 is sufficient to induce high rates of intestinal small cell carcinoma and adenocarcinoma, establishing the utility of our transgenic zebrafish model in the study of CagA-associated gastrointestinal cancers. (zfin.org)
Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)

But nonviral tumors, which are composed of aberrant host cells, do not provide such targets, and the immune response is suppressed by defenses against autoimmune reactions. (the-scientist.com)
Despite significant progress, these protocols remain limited by low yield and purity of the desired mature cell types, as well as the potential of immature cells to form tumors. (eurekalert.org)

According to the author, 'PAHAT enables precise non-contact manipulation of cells in living organisms. (sciencemission.com)
In multi-cellular organisms, cells continuously monitor their environment for nutrient and signal availability (mitogens, trophic factors, stress) to decide whether to self-renew, proliferate, differentiate or die. (iric.ca)
The resulting clones are genetically identical organisms containing the recombinant DNA. (thehealthboard.com)

Co-expression of CagA with a loss-of-function allele encoding the β-catenin destruction complex protein Axin1 resulted in a further increase in intestinal proliferation, while co-expression of CagA with a null allele of the key β-catenin transcriptional cofactor Tcf4 restored intestinal proliferation to wild-type levels. (zfin.org)
We demonstrate here that GSK-3 maintains the MLL leukemia stem cell transcriptional program by promoting the conditional association of CREB and its coactivators TORC and CBP with homedomain protein MEIS1, a critical component of the MLL-subordinate program, which in turn facilitates HOX-mediated transcription and transformation. (stanford.edu)
We study transcriptional regulation, cell signaling, and the interaction of these. (umich.edu)
In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)

His laboratory developed several models of genetically altered mice to study the pathophysiological role of these enzymes. (iric.ca)
Moreover, these cells showed potential as a cell replacement therapy in mice with cystic fibrosis. (eurekalert.org)
The researchers started to genetically reprogram adult Club cells isolated from mice, transiently expressing the four iPS reprogramming factors, but interrupted the process early, prior to reaching the pluripotent state, to generate progenitor-like cells, which are more committed to a specific lineage and show more controlled proliferation than pluripotent cells. (eurekalert.org)
When the Club-iPL cells were administered to CFTR-deficient mice, the cells incorporated into tissue lining the respiratory tract and partially restored levels of CFTR in the lungs without inducing tumor formation. (eurekalert.org)
Consistent with this notion, we have found that Smurf2-deficient mouse embryonic fibroblasts exhibit delayed senescence entry and enhanced potential to become immortalized in culture, while Smurf2-deficient mice show increased susceptibility to various types of cancer, including B-cell lymphoma, hepatocellular carcinoma, adenocarcinoma in small intestine and soft tissue sarcoma. (umassmed.edu)
Genetically engineered mice that model human disease are also valuable for testing therapeutic interventions. (umich.edu)

These data are then used to identify both genetically distinct subtypes of sarcoma and molecular signatures associated with tumor morphology, grade, recurrence, and survival. (mskcc.org)
In vivo development and survival of NK cells require cytokines ( 6 - 8 ). (frontiersin.org)
B cell receptor (BCR) signaling plays a critical role in driving proliferation and survival of the malignant clone in chronic lymphocytic leukemia (CLL), supported by the clinical activity of inhibitors targeted toward BCR-associated kinases [ 1 ]. (nature.com)
Macroautophagy (hereafter referred to as autophagy) plays an important role in cancer cells survival upon various conditions of intra- and extracellular stress. (aging-us.com)
The VeraVec TM cells form a nurturing niche for the survival and growth of the reprogrammed blood cells, similar to what happens developmentally during blood production. (businesswire.com)

Their normal function is not to cause cancer but to participate in the regulation of normal cell division. (the-scientist.com)
Sylvain Meloche's team particularly focuses on mode of operation, regulation and kinase proteins targets of the MPA kinases family, mainly ERK1/2 and ERK3/4, known as essential regulators of cell proliferation, and kinases of the SRC family. (iric.ca)
They are known to be involved in the regulation of NK and T cell responses, mostly by homotypic interactions except for SLAMF2 (CD48) and SLAMF4 that interact with each other [ 10 ]. (nature.com)
GSH is involved in the regulation of a number of essential biochemical processes within the cell. (immunochemistry.com)
In its role as a cell signaling agent, GSH is involved in DNA synthesis and cell proliferation regulation. (immunochemistry.com)
We are studying the role that normal chromatin structure plays in gene regulation in hematopoietic cells and how its disruption leads to altered development and cancer. (stanford.edu)
Research on vitamin D regulation of thymocyte selection, Th1 and Th17 cells, T-cell programed cell death, and T-regulatory (Treg) cells is summarized and integrated into model mechanisms. (frontiersin.org)

However, somatic tissues are mainly comprised of heterogeneous, differentiated cell lineages that can be short lived and difficult to specifically transfect. (jci.org)
Cutaneous T-cell lymphoma (CTCL) (see the image below) is a heterogeneous group of lymphoproliferative disorders characterized by localization of neoplastic T lymphocytes to the skin, with no evidence of extracutaneous disease at the time of diagnosis. (medscape.com)
In highly heterogeneous tumours, like pancreatic ductal adenocarcinoma (PDAC) however, the characterisation of cancer stem cells has led to controversial results due to the lack of consensus on specific markers. (lu.se)
Osteosarcomas with few chromosomal alterations or adult onset are genetically heterogeneous. (lu.se)

They can be activated rapidly via germ-line encoded receptors that recognize the presence of stress ligands or absence of self-antigens on target cells ( 1 - 5 ). (frontiersin.org)
1 , 2 The anti-tumor effect of DLI is mediated by donor T cells recognizing allo-antigens on the malignant cells of the recipient. (haematologica.org)
4 In addition, TCRs for minor histocompatibility antigens, including HA-1 and HA-2, have been transferred to donor-derived virus-specific T cells to treat patients with hematologic malignancies after allogeneic stem cell transplantation. (haematologica.org)
Tumor Antigens Many tumor cells produce antigens, which may be released in the bloodstream or remain on the cell surface. (msdmanuals.com)
Concomitant use of interferon enhances the expression of major histocompatibility complex (MHC) antigens and TAAs on tumor cells, thereby augmenting the killing of tumor cells by the infused effector cells. (msdmanuals.com)

Directed gene transfer into specific cell lineages in vivo is an attractive approach for both modulating gene expression and correcting inherited mutations such as emphysema caused by human α1 antitrypsin (hAAT) deficiency. (jci.org)
Sarcoma tissue samples and cell lines are subjected to a multiplatform genome-wide characterization of expression of protein-coding genes and microRNAs, DNA copy number changes, activating mutations, gene rearrangements and methylation profiling. (mskcc.org)
Senescence limits proliferative capacity of cells and thus impedes the accumulation of multiple mutations necessary for tumorigenesis. (umassmed.edu)
In histiocytic proliferations the distribution of BRAF mutations and their relevance has not been clarified. (oncotarget.com)
In conclusion, BRAF mutations in histiocytic proliferations are restricted to lesions of the Langerhans-cell type. (oncotarget.com)

Scientists working in basic, translational, and clinical cancer metabolism research are invited to join the Academy in New York on April 17th to discuss the intersection between cell signaling and metabolism. (nyas.org)
Cancer Cell Metabolism: Unique Features Inform New Therapeutic Opportunities. (nyas.org)

Our results challenge the dogma that AMs are short lived and suggest that these differentiated cells may be a possible target cell population for in vivo gene therapy applications, including the sustained correction of hAAT deficiency. (jci.org)
CCD camera imaging demonstrated in vivo expression of the luciferase reporter gene, while flow cytometry of cells obtained by BAL from this mouse demonstrated expression of GFP in 33% of cells. (jci.org)
The use of CD20 as suicide gene was investigated in CMV specific T cells and in T cells genetically modified with an antigen specific T-cell receptor. (haematologica.org)
Herpes simplex virus thymidine kinase (HSV-tk) is a well-established suicide gene that has been successfully used to control GvHD following donor lymphocyte infusions (DLI) after allogeneic stem cell transplantation. (haematologica.org)
T-cell receptor (TCR) gene transfer is an attractive strategy for rapid in vitro generation of high numbers of antigen specific T cells. (haematologica.org)
Although human CD20 has previously been described as functional selection marker and suicide gene after retroviral transfer to T lymphocytes, 9 , 10 the specificities of these T cells were unknown, and therefore a potential effect of CD20 on antigen-specific T-cell functions could not be investigated. (haematologica.org)
1 Center for Cell and Gene Therapy, Baylor College of Medicine, Texas Children's Hospital and Houston Methodist Hospital. (aacrjournals.org)
In addition, TGT inactivation via gene knockout leads to the suppressed cell proliferation and migration of certain breast cancer cells, which may render this enzyme a potential target for the design of compounds supporting breast cancer therapy. (rcsb.org)
Gene-environment interactions, sunlight and vitamin D, and T lymphocytes as autoimmune disease initiators and vitamin D targets are discussed to explain the rationale for reviewing vitamin D mechanisms in T cells. (frontiersin.org)
In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)

However, direct injection of IL-2 has been shown to be accompanied by severe side effects, such as vascular leak syndrome, activation-induced cell death, and strong induction of regulatory CD4 pos T cells, which did not occur after IL-15 administration ( 15 , 16 ). (frontiersin.org)
Her work also contributed to the development of foundational hypotheses on the role of smooth muscle cell contractility in thoracic aortic disease and smooth muscle cell proliferation in vascular occlusive diseases. (uth.edu)

In particular, quinolone-like compound 91 (C91) at a non-toxic nanomolar concentration reduced mSIN3b nuclear entry and occupancy at the RE1/NRSE within the Bdnf locus, and restored brain-derived neurotrophic factor (BDNF) protein levels in HD cells. (ox.ac.uk)

Therapies aimed at targeting ERK1/2 likely fail because this mechanism is allowing proliferation through a different pathway," noted senior study author Eyal Raz, M.D., professor of medicine at UC San Diego School of Medicine. (genengnews.com)
Autoimmune side effects, however, remain a risk, emphasizing the relevance of a suicide mechanism allowing in vivo elimination of infused T cells. (haematologica.org)
Adverse side effects, however, may occur and lead to severe autoimmunity or graft-versus-host disease (GvHD), emphasizing the relevance of a suicide mechanism that allows efficient in vivo elimination of infused T cells. (haematologica.org)
This mechanism also applies to hematopoietic cells transformed by other HOX genes, including CDX2, which is highly expressed in a majority of acute myeloid leukemias, thus providing a molecular approach based on GSK-3 inhibitory strategies to target HOX-associated transcription in a broad spectrum of leukemias. (stanford.edu)
Furthermore, aberrant oncogenic activation, DNA damage or oxidative stress activates senescence, providing a failsafe mechanism that prevents the proliferation of cells at risk of neoplastic transformation. (umassmed.edu)
We use two main approaches in our birth defects research - sequencing patient DNA to identify novel disease genes and use of cell culture and animal models, especially the mouse, to understand the mechanism and pathophysiology of disease. (umich.edu)

Our scientists pursue every aspect of cancer research-from exploring the biology of genes and cells, to developing immune-based treatments, uncovering the causes of metastasis, and more. (mskcc.org)
1 Program in Cancer and Stem Cell Biology, Duke-NUS Graduate Medical School, Singapore, Singapore. (nih.gov)
Since SLAMF receptors play a role in NK cell biology, we reasoned that these receptors may impact NK cell-mediated CLL immunity. (nature.com)
Kwartler received her doctorate of philosophy in biomedical sciences (cell and regulatory biology) in 2013 from The University of Texas MD Anderson UTHealth Houston Graduate School of Biomedical Sciences. (uth.edu)
Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)

This whole cell glutathione assay employs a proprietary thiol-sensitive dye, ThioBright™ Green, to monitor relative changes in the concentration of the free form of glutathione, aka GSH. (immunochemistry.com)
The key assay reagent is a proprietary thiol-sensitive dye, ThioBright™ Green, which quickly penetrates cell membrane structures and accumulates primarily within the cytosol of living cells. (immunochemistry.com)
Optional: Spin and pellet cells, remove supernatants, and gently resuspend in Assay Buffer or other isotonic buffer. (immunochemistry.com)
Pig bone marrow mononuclear cells had been remoted and cultured in EGM-2 medium to acquire bone marrow-derived EPCs (BM-EPCs) that had been characterised by immunostaining and the tube formation assay. (inacj.com)
Biological activity of ABD-GCSF protein was assessed by proliferation assay on NFS-60 cells. (sciencegate.app)
There was a greater level of apoptotic activity in the airway biopsy in subjects with severe asthma as compared with the normal subjects using the TUNEL assay (p = 0.002), suggesting increased cell death. (atsjournals.org)

Peripheral blood mononuclear cells (PBMC) were collected from healthy donors after approval by the LUMC institutional review board and informed consent according to the Declaration of Helsinki. (haematologica.org)
In a study of patients with type II cryoglobulinemia, peripheral blood mononuclear cells from 18 patients were separated into CD3+ (T cells), CD19+ (B cells), and CD14+ (monocytes) and analyzed for the presence of negative-strand HCV RNA and for HCV nonstructural protein 3 (NS3). (medscape.com)

New therapeutic concepts in anti-tumor therapy aim to modulate the patient's immune system to increase its aggressiveness or targeted effects toward tumor cells. (frontiersin.org)
In a proof of concept in vivo study, we also observed a therapeutic effect of adoptively transferred IL-15 expanded and IL-21 boosted NK cells in combination with image guided high precision radiation therapy using a luciferase-transduced RMS xenograft model. (frontiersin.org)
CD20-transduced T cells with and without co-transferred T-cell receptor were efficiently eliminated by complement dependent cytotoxicity induced by therapeutic anti-CD20 antibody rituximab. (haematologica.org)
A easy, simple and low-cost methodology that may be utilized to supply numerous genetically-modified BM-EPCs was established, which is able to facilitate the research of GTE-EPC therapeutic efficacy in pig MI mannequin. (inacj.com)
Apart from DNA cloning, two other main cloning types are reproductive cloning, which is concerned with human and animal cloning, and therapeutic cloning, concerned with embryonic cloning to harvest stem cells for research and potential medical treatment purposes. (thehealthboard.com)
Cancer stem cells are a population of slow-cycling cells within the tumour bulk, with self-renewal capacity that attracts interest as a therapeutic target. (lu.se)

In recent years, induced pluripotent stem (iPS) cells have generated a great deal of interest as a potentially unlimited source of various cell types for transplantation. (eurekalert.org)
Moreover, there is no standardized approach applicable to all cell types, and the development of personalized therapies based on patient-derived pluripotent cells remains very expensive and time consuming. (eurekalert.org)
This led us to question whether excessive Ca V 3 channels, retained mistakenly in engineered human-induced pluripotent stem cell-derived islet (hiPSC-islet) cells, act as an obstacle to hiPSC-islet maturation. (mdpi.com)

The aim of adoptive T-cell therapy of cancer is to selectively confer immunity against tumor cells. (haematologica.org)

The goal of our laboratory is to develop, scale up, and broadly disseminate molecular technologies for mapping cells and functional circuits. (stanford.edu)
Due to the genetically conserved molecular processes by which cells die, intracellular levels of GSH can favor a cell death pathway via apoptosis (adequate intracellular GSH stores) or via necrosis or autophagy (depleted intracellular GSH stores). (immunochemistry.com)
Finally, unanswered questions relating to vitamin D mechanisms in CD4 + T cells are highlighted to promote further research that may lead to a deeper understanding of autoimmune disease molecular etiology. (frontiersin.org)
Adipogenesis has been extensively studied in mammals [ 7 ] and several cell and animal models have been successfully used to describe the chronology of the molecular events governing this process. (biomedcentral.com)

Furthermore, targeting both pathways causes a more effective suppression of cell proliferation in murine intestinal organoids and human CRC lines. (genengnews.com)
The successful strategy based on the activation of cell death in Ras-expressing cells is associated with the suppression of kinases involved in Ras pathway. (aging-us.com)
Our data show that suppression of MEK/ERK pathway in senescent cells provides a new strategy for elimination of Ras-expressing cells. (aging-us.com)
0.001), indicating decreased cell death suppression. (atsjournals.org)

We studied whether cellular senescence induced by HDAC inhibitor sodium butyrate in E1a+cHa-Ras -transformed rat embryo fibroblasts (ERas) and A549 human Ki-Ras mutated lung adenocarcinoma cells would enhance the tumor suppressor effect of MEK/ERK inhibition. (aging-us.com)
2008). Historically, this concept is highlighted by the experi- factors are key intrinsic regulators of these fate decisions and mental phenomenon of lineage reprogramming, for example, that fate choice involves modulating networks of transcription by the conversion of fibroblasts to muscles cells following trans- factors. (lu.se)

Since the SLAMF low signature was strongly associated with an unmutated CLL immunoglobulin heavy chain (IGHV) status in large datasets, we investigated the impact of SLAMF1 and SLAMF7 on the B cell receptor (BCR) signaling axis. (nature.com)
Notably, inhibition of GPR171 synergistically enhanced the tumoricidal activity of an epidermal growth factor receptor (EGFR) inhibitor in lung cancer cells. (oncotarget.com)

Finally, Professor Meloche's team has long been interested in the ubiquitin-proteasome system of protein degradation and its role in cell signalling and cell proliferation. (iric.ca)
Despite potential roles in the tumorigenesis of lung and breast cancer cells, the atypical protein kinase ERK3, a member of the Ras/MAPK pathway, is poorly characterized. (iric.ca)
The researchers showed that the resulting Club-iPL cells could give rise to not only Club cells, but also to other respiratory tract cells such as mucus-secreting goblet cells and ciliated epithelial cells that produce the CFTR protein, which is mutated in patients with cystic fibrosis. (eurekalert.org)
Glutathione, γ-L-glutamyl-L-cysteinyl-glycine or GSH, is the most abundant non-protein thiol in cells. (immunochemistry.com)
Levels of retinoblastoma protein (Rb), Ki67, and Bcl-2 were measured, reflecting cellular proliferation and death. (atsjournals.org)
Proliferation was characterized by enrichment in genes involved in basic cellular and metabolic processes (transcription, ribosome biogenesis, translation and protein folding), cellular remodelling and autophagy. (biomedcentral.com)
NS3 protein was also detected in 6 patients: 5 were positive in T cells, 3 in B cells, and 3 in monocytes. (medscape.com)

Kwartler also studied the mechanisms of pediatric-onset cerebrovascular disease, identifying a completely novel role for smooth muscle α-actin in the nucleus and showed that loss of this specific nuclear function impairs the cell fate specification of smooth muscle cells. (uth.edu)

Here we investigated the characteristics of a population of pancreatic cancer tumorspheres derived from different human pancreatic cancer cell lines and a primary line from a genetically engineered KPC mouse model, using flow cytometry and western blotting to analyse surface and stemness markers. (lu.se)

A major block in the critical path of regenerative medicine is the lack of suitable cells to restore function or repair damage," says co-senior author Tom Waddell, a thoracic surgeon at the University of Toronto. (eurekalert.org)
According to the authors, the approach could be used for a variety of regenerative medicine practices, including cell replacement therapy, disease modelling, and drug screening for human diseases. (eurekalert.org)
Because these cells secrete and display factors essential for stem cell growth and proliferation, they can be used to support cell-based therapies, stem cell transplant, and regenerative medicine applications. (businesswire.com)

Scaffolds for tissue regeneration must present a viable microenvironment for the living cells to adhere, proliferate, and exhibit the necessary tissue function. (asme.org)
Biocompatible materials, such as polycaprolactone (PCL), have been used extensively to fabricate scaffolds using electrospinning technique, to study cell compatibility and to evaluate cell functionality for nerve tissue engineering applications. (asme.org)
Immunofluorescent staining of intestinal epithelium tissue shows cell growth (green). (genengnews.com)
The ERK pathway plays a critical role in embryonic development and tissue repair because it instructs cells to multiply and start dividing, but when overactivated cancer growth often occurs. (genengnews.com)
For example, normal stroma, the connective material that supports the cells of a tissue, appears to inhibit cancer growth. (the-scientist.com)
Due to their high tissue penetrability and strong acoustic radiation force, such tweezers overcome the limitations of optical and magnetic tweezers, thus making them suitable for in-vivo cell manipulation. (sciencemission.com)
Combining with functional cells and cell spheroids, it has great potential in immunotherapy, tissue engineering, targeted drug delivery, and other fields. (sciencemission.com)
Beyond the single cell, we also strive to map cellular ensembles, such as brain tissue. (stanford.edu)
We develop tools that can be used to construct cell and tissue atlases, and map the cellular circuits that give rise to function and behavior. (stanford.edu)
We are interested in whether age-dependent accumulation of senescent cells leads to alterations in tissue microenvironment that is favorable for oncogenesis. (umassmed.edu)
Mariano García Arranz has the following conflict of interest: MGA is inventor on 2 patents entitled "Identification and isolation of multipotent cells from non-osteochondral mesenchymal tissue" (10157355957US) and "Use of adipose tissue-derived stromal stem cells in treating fistula" (US11/167061). (wjgnet.com)
We have previously shown that fish primary preadipocytes differentiate into mature adipocytes in vitro and that these cells represent a very helpful model system to study adipose tissue development in fish [ 5 , 6 ]. (biomedcentral.com)
Low power, H and E showing a hypocellular bone marrow with increased adipose tissue and decreased hematopoietic cells in the marrow space. (medscape.com)

Evidence for CD4 + T-cell involvement in autoimmune disease pathogenesis and for paracrine calcitriol signaling to CD4 + T lymphocytes is summarized to support the thesis that calcitriol is sunlight's main protective signal transducer in autoimmune disease risk. (frontiersin.org)
Animal modeling and human mechanistic data are summarized to support the view that vitamin D probably influences thymic negative selection, effector Th1 and Th17 pathogenesis and responsiveness to extrinsic cell death signals, FoxP3 + CD4 + T-regulatory cell and CD4 + T-regulatory cell type 1 (Tr1) cell functions, and a Th1-Tr1 switch. (frontiersin.org)
During her postdoctoral work, Kwartler studied mechanisms of pathogenesis for genetically triggered thoracic aortic aneurysms and dissections. (uth.edu)

The normal function of the proteins expressed by these genes is to recognize superfluous, damaged, aged, or aberrant cells that must be eliminated. (the-scientist.com)
Our recent studies as well as others indicate that senescent cells change their secretory patterns of cytokines, chemokines, growth factors, extracellular matrix proteins and proteases. (umassmed.edu)
Chimeric antigen receptors (CAR) recognize specific proteins on the surface of tumor cells. (msdmanuals.com)
In contrast to TCR T cells, CAR T cells recognize only relatively large proteins on the surface of tumor cells. (msdmanuals.com)

The current research aimed to analyze the impression of H1975 cell crosstalk with PLTs on the proliferation, migration and tube formation of HUVECs. (inacj.com)
As well as, the next relative migration price, better variety of migrated cells, stronger tube-forming means and elevated expression of VEGF and VEGF R2 had been detected within the Exp_HP group in contrast with the management group. (inacj.com)
Knockdown of GPR171 also inhibited migration and invasion of the lung cancer cell lines. (oncotarget.com)

The mutated genes responsible for this proliferation are called cancer genes, or oncogenes, but this is a misnomer. (the-scientist.com)

Tumorigenesis is a multi-step process in which a normal cell acquires changes in a number of critical cancer causing genes. (umassmed.edu)

Sylvain Meloche's laboratory uses an interdisciplinary approach to understand how signalling pathways control the fate of normal and cancer cells. (iric.ca)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)

Acute leukemias induced by MLL chimeric oncoproteins are among the subset of cancers distinguished by a paradoxical dependence on GSK-3 kinase activity for sustained proliferation. (stanford.edu)
In many human cancers, misregulated EZH2 enzyme activity results in misregulation of genes that control cell proliferation-without these control mechanisms, cancer cells are free to grow rapidly. (shu.edu)
and large cell carcinoma (5-15% of all lung cancers) [ 4 , 5 ]. (oncotarget.com)

Since 1994, ImmunoChemistry Technologies has been producing top quality reagents, kits, and services for cell viability and ELISA applications in a broad range of research areas. (immunochemistry.com)

Symptoms of CNS involvement are rarely noted at initial diagnosis but are more common in T-lineage and mature B cell ALL. (medscape.com)

Genotyping of 69 histiocytic lesions revealed that 23/48 Langerhans cell lesions were BRAF -V600E-mutant whereas all non-Langerhans cell lesions (including dendritic cell sarcoma, juvenile xanthogranuloma, Rosai-Dorfman disease, and granular cell tumor) were wild-type. (oncotarget.com)

Meanwhile, more-appropriately named tumor suppressor genes recognize the illegitimate activation of proliferation-driving genes and hit the brakes. (the-scientist.com)

The importance of apoptosis is exemplified by the vertebrate adaptive immune system, in which B lymphocytes, as they differentiate from their precursor cells, rearrange the DNA of immunoglobulin genes to generate a vast number of potential antibodies. (the-scientist.com)

This scientific advance, reported in the July 2 issue of Nature , could potentially lead to therapies for patients with blood disorders from their own cells. (businesswire.com)
Clinical trials of LAK cells in humans are ongoing but this approach has not gained widespread use and is generally considered less effective than other cell therapies. (msdmanuals.com)

The image below depicts bone marrow aspirate from a child with T-cell acute lymphoblastic leukemia. (medscape.com)
A particularly important aspect of this study was that the reprogrammed cells engrafted in the bone marrow when implanted into rodents and morphed into the various types of blood cells," said Dr. Rafii. (businesswire.com)
The clinical presentation of patients with aplastic anemia includes symptoms related to the decrease in bone marrow production of hematopoietic cells (see the image below). (medscape.com)

Depletion of GPR171 with an anti-GPR171 antibody decreased proliferation of lung carcinoma cells and attenuated tumor progression in a mouse xenograft model. (oncotarget.com)

To achieve these goals, our laboratory has focused on the development of scalable technologies to detect, measure, and manipulate molecules and circuits, both at the sub-cellular level, and at the level of cell populations. (stanford.edu)
The expansion of cell populations is a strictly controlled process that results from the balance between cell division and cell death. (iric.ca)
As demonstrated November 30 in the journal Stem Cell Reports , researchers in Canada converted adult mouse respiratory tract cells called Club cells into large, pure populations of induced progenitor-like (iPL) cells, which retained a residual memory of their parental cell lineage and therefore specifically generated mature Club cells. (eurekalert.org)
This technology can theoretically be applied to almost any cell type that can be isolated and purified, and isolation of highly purified populations of adult cells from most organs is already possible with existing techniques. (eurekalert.org)

This method involves genetically reprogramming skin cells taken from adult donors to an embryonic stem-cell-like state, growing these immature cells to large numbers, and then converting them into specialized cell types found in different parts of the body. (eurekalert.org)
We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)

Besides surgery, radiotherapy and chemotherapy, immune activation by direct application of cytokines, antibodies or adoptive cell therapy are promising approaches. (frontiersin.org)
With their ability to detect and directly destroy virally infected or malignant cells, natural killer (NK) cells form an important part of the first line defense of the immune system. (frontiersin.org)
A total of 45 CNVs were found in four lines of chickens, and 28 were potentially involved in immune response and cell proliferation, etc. (usda.gov)
Together, our data indicate that SLAMF receptors are important modulators of the BCR signaling axis and may improve immune control in CLL by interference with NK cells. (nature.com)
Signaling Lymphocytic Activation Molecule Family (SLAMF) receptors are a group of nine type I transmembrane receptors that are mainly expressed on a variety of immune cells. (nature.com)
A major advantage of this approach is the ability to generate patient-specific iPS cells for transplantation, thereby minimizing the risk of harmful immune reactions. (eurekalert.org)

Treatment of control ERas cells with PD0325901 for 24 h results in mitochondria damage and apoptotic death of a part of cellular population. (aging-us.com)
0.01) in the epithelium of subjects with severe asthma as compared with normal subjects, indicating increased cellular proliferation. (atsjournals.org)
In subjects with severe asthma, as compared with subjects with mild asthma, normal subjects, and diseased control subjects, we found novel evidence of increased cellular proliferation in the airway contributing to a thickened epithelium and LR. (atsjournals.org)
In severe asthma, there is increased cellular proliferation in the airway contributing to a thickened epithelium and lamina reticularis. (atsjournals.org)
In passive cellular immunotherapy, specific effector cells are directly infused and are not induced within the patient. (msdmanuals.com)
Passive cellular immunotherapy references A number of immunologic interventions, both passive and active, can be directed against tumor cells. (msdmanuals.com)
In passive cellular immunotherapy, specific effector cells. (msdmanuals.com)

These stem cells have renowned ability for use in cell therapy and organ regeneration. (asme.org)
Such studies will enable us to understand the effects of drugs such as valproic acid on stem cells and will aid in designing scaffolds for applications in nerve regeneration. (asme.org)

A prolonged macrophage lifespan, rather than precursor cell proliferation, accounted for the surprisingly sustained presence of transduced AMs. (jci.org)
It is my personal opinion milk is a healthy product only if it is raw milk straight out of a cow which is pasture/hay fed, not given steroids or antibiotics that affect their health and lifespan, and are not fed GMO genetically modified food. (detailshere.com)

A modified version of iPS methodology, called interrupted reprogramming, allows for a highly controlled, potentially safer, and more cost-effective strategy for generating progenitor-like cells from adult cells. (eurekalert.org)
Despite intensive research, there is a distinct lack of methodology for visualizing endogenous ubiquitination in living cells. (nature.com)

T-cell clones were stimulated every 10-14 days with irradiated allogeneic PBMC and phyto-hemagglutinin in IMDM with 5% human serum (HS), 5% FBS and 100 IU/mL IL-2 as previously described. (haematologica.org)

Providing no event occurs, the resulting daughter cells are genetically identical to the original cell. (wikipedia.org)

The exciting part of this new study is investigators found that treating both ERK1/2 and the compensatory pathway ERK5 concomitantly with a combination of drug inhibitors halted CRC growth more effectively in both mouse models and human CRC cell lines. (genengnews.com)
The researchers were excited by their findings but urged caution at over interpretation of their initial findings and suggested that other classes of inhibitors be tested in combination with ERK5 inhibitors in human CRC cells in preclinical mouse models before any patient trial can begin. (genengnews.com)
For their own part, the researchers plan to test this approach with other cell types, including human cells. (eurekalert.org)
CAR T cell immunotherapy for human cancer. (msdmanuals.com)

Wharton's jelly MSC are multipotent adult stem cells present in the umbilical cord and drawn from their matrix [1,2,3]. (asme.org)

The transfected cells are cultured and proliferation of the recombinant DNA takes place. (thehealthboard.com)

Cancer cells exploit a variety of protective mechanisms that allow them to acquire selective advantage and proliferate under unfavorable conditions. (aging-us.com)
One of the mechanisms of high basal autophagy activity in cancer Ras-expressing cells might be associated with high PP2A phosphatase activity targeting directly ULK1-Ser757 that could explain apparent contradiction: maintenance of high mTORC1 functions and high autophagic activity simultaneously [ 22 ]. (aging-us.com)

The findings from this study were published recently in Nature Communications in an article entitled "ERK5 Signalling Rescues Intestinal Epithelial Turnover and Tumour Cell Proliferation upon ERK1/2 Abrogation. (genengnews.com)

These genes recognize and stop the illegitimate activation of proliferation-driving genes. (the-scientist.com)
Neal, J.T., Peterson, T.S., Kent, M.L., and Guillemin, K. (2013) H. pylori Virulence Factor CagA Increases Intestinal Cell Proliferation by Wnt Pathway Activation in a Transgenic Zebrafish Model. (zfin.org)
CNK2 promotes cancer cell motility by mediating ARF6 activation downstream of AXL signalling. (iric.ca)
In B cells, this class of receptors has been shown to be expressed in distinct patterns associated with development and activation [ 11 ]. (nature.com)

Senescent ERas cells do not develop cytoprotective autophagy upon inhibition of MEK/ERK pathway due to spatial dissociation of lysosomes and autophagosomes in the senescent cells. (aging-us.com)
Senescent cells are unable to form the autophagolysosomes and to remove the damaged mitochondria resulting in apoptotic death. (aging-us.com)

In this protocol, we describe how unique properties of ubiquitin (Ub)-binding domains (UBDs) can be used to selectively detect, visualize and inhibit Ub-dependent processes in mammalian cells. (nature.com)

The specific medications administered depend on the choice of therapy and whether it is supportive care only, immunosuppressive therapy, or hematopoietic cell transplantation. (medscape.com)
Central venous catheter placement is required before the administration of hematopoietic cell transplantation. (medscape.com)

We have pursued cell therapy for lung diseases for many years," Waddell says. (eurekalert.org)
There are two clinical types of lung cancer: non-small cell lung carcinoma (NSCLC), which accounts for 85% of all cases, and small cell lung carcinoma (SCLC) [ 1 , 3 ]. (oncotarget.com)

Anatomy42

Organisms13

Diseases13

Chemicals and Drugs100

Analytical, Diagnostic and Therapeutic Techniques and Equipment33

Phenomena and Processes56

Disciplines and Occupations1

Information Science3

Differentiation19

Transferred genes1

Intestinal epithelial cell proliferation2

Mesenchymal stem2

Diffuse large B-2

Progenitor cells4

Endothelial cells6

Genetic7

Bacterial4

Induce4

Tumors2

Organisms3

Transcriptional4

Mice6

Survival5

Regulation7

Heterogeneous4

Antigens5

Mutations5

Metabolism2

Gene10

Vascular2

Brain-Derive1

Mechanism6

Biology5

Assay6

Mononuclear cells2

Therapeutic6

Pluripotent3

Immunity1

Molecular4

Suppression4

Fibroblasts2

Receptor2

Protein7

Impairs1

Flow cytometry1

Regenerative medicine3

Tissue13

Pathogenesis3

Proteins4

Migration3

Cancer genes1

Tumorigenesis1

Pathways2

Cancers3

ELISA1

Involvement1

Sarcoma1

Tumor suppr1

Precursor cells1

Therapies2

Bone marrow3

Antibody1

Populations4

Embryonic2

Immune6

Cellular7

Regeneration2

Lifespan2

Methodology2

Clones1

Identical1

Human4

Adult1

Recombinant1

Mechanisms2

Turnover1

Activation4

Senescent cells2

Mammalian cells1

Hematopoietic cell2

Lung2