• CDC scientists used whole genome sequencing (WGS) on the Campylobacter bacteria isolated from sick people's stool samples. (cdc.gov)
  • Whole genome sequencing by CDC determined that the strain of virus making the rats and people sick was closely related to a strain of Seoul virus previously found in pet rats in the United Kingdom. (cdc.gov)
  • strain RB-44 complete genome sequence using PacBio single-molecule real-time sequencing technology. (genomeweb.com)
  • Improved hybrid genome assemblies of two strains of Bacteroides xylanisolvens, SD_CC_1b and SD_CC_2a, obtained using Illumina and 454 sequencing technologies. (genomeweb.com)
  • Full genome virus detection in fecal samples using sensitive nucleic acid preparation, deep sequencing, and a novel iterative sequence classification algorithm. (genomeweb.com)
  • Quantifying genome-editing outcomes at endogenous loci with SMRT sequencing. (genomeweb.com)
  • The FDA's 2009 "Guidance on Prophylactic DNA Vaccines: Analysis and Recommendations" states that concerns about plasmid DNA potentially integrating into the genome of the vaccine recipient and increasing the likelihood of issues like "malignant transformation, genomic instability, or cell growth dysregulation" were raised when DNA vaccines were initially introduced for clinical use. (zerohedge.com)
  • The complete genome sequence of Archeoglobus fulgidus is published. (jcvi.org)
  • Helicobacter pylori genome sequence published. (jcvi.org)
  • Twenty years ago, President Bill Clinton announced completion of what was arguably one of the greatest advances of the modern era: the first draft sequence of the human genome. (jcvi.org)
  • Lane 2: Amplifi-cation cagA gene from coccoid H pylori genome DNA by PCR. (wjgnet.com)
  • The Ti plasmid contains virulence genes (vir), which code for the protein machinery required for transfer and integration of disease causing genes into the host plants genome. (igem.org)
  • Any DNA sequences situated between the LB and RB will be inserted into the plant host genome. (igem.org)
  • Once integrated into the host genome, these genes, referred to as the oncogenes, hijack the host plant to produce the protein machinery needed for the production of phytohormones. (igem.org)
  • By swapping the oncogenes from the T-DNA region of the Ti plasmid with any plant gene expression cassette (containing a plant promoter, a gene of interest and a transcriptional terminator sequence), they were able to integrate their particular gene of interest into the host genome without causing the disease. (igem.org)
  • Sequence-directed genetic interference pathways control gene expression and preserve genome integrity in all kingdoms of life. (rockefeller.edu)
  • This study reveals a new mechanism for the fluid gain of beneficial mutations in genetic regions undergoing active recombination in viruses and illustrates the value of long read sequencing technologies for investigating complex genome dynamics in diverse biological systems. (elifesciences.org)
  • It is very important to have the genome sequenced for Y. pestis because this organism is capable of causing very fatal diseases. (kenyon.edu)
  • Since scientists were able to sequence the genome, they now have information of how diseases caused by this pathogenic bacteria are developed and also the evolutionary history of the bacteria. (kenyon.edu)
  • Having the genome sequenced also means that they are able to determine other species that are related to yersinia pestis which can prevent future outbreaks. (kenyon.edu)
  • The genome has many insertion sequences and many G-C base pair differences, which means frequent recombination. (kenyon.edu)
  • High transformation efficiency of citrus is of great importance for large scale characterization of gene functions and also cultivar development via transgenic and genome editing technologies. (springer.com)
  • Twenty ESBL producing strains (15%) including Escherichia coli (n = 9), Klebsiella pneumoniae (n = 7), Klebsiella oxytoca (n = 2) and Enterobacter aerogenes (n = 2) were detected and further analyzed for their resistance transfer features, plasmid profile and nature of the resistance genes. (scielo.br)
  • To further investigate the genomic diversity among this group and to help characterize lineages of the plague organism that have no sequenced members, we present here the genomes of two isolates of the "classical" antiqua biovar, strains Antiqua and Nepal516. (asm.org)
  • Since extraintestinal pathogenic Escherichia coli (ExPEC) strains from human and avian hosts encounter similar challenges in establishing infection in extraintestinal locations, they may share similar contents of virulence genes and capacities to cause disease. (unboundmedicine.com)
  • Also, 58% belonged to the ST95 multilocus sequence typing group, and over 90% of them were assigned to the B2 phylogenetic group typical of human ExPEC strains. (unboundmedicine.com)
  • This cluster contained strains with a high number of both chromosome- and plasmid-associated ExPEC genes. (unboundmedicine.com)
  • Also, the widespread occurrence of plasmids among NMEC strains and members of the mixed cluster suggests that plasmid-mediated virulence in these pathotypes warrants further attention. (unboundmedicine.com)
  • In pathogenic strains of ''A. tumefaciens'', the border sequences flank genes involved in tumor formation in infected plants. (igem.org)
  • The strains positive in PCR for subjected to 6 different PCR reactions tar- pCVD432 were interpreted as EAEC, and geting STEC (stx1 and stx2 genes), ETEC those positive for stx genes as STEC. (who.int)
  • LT and ST toxin-producing genes), EPEC strains positive in PCR for LT, ST or both (eae gene), and EAEC (pCVD432 plasmid) were considered ETEC. (who.int)
  • Here, we used a combination of long- and short-read sequence data of Klebsiella pneumoniae isolates (n = 1,717) from a European survey to perform an integrated, continent-wide study of chromosomal and plasmid diversity. (ox.ac.uk)
  • The aims of this research work were to determine the patterns of antibiotic resistance in Escherichia coli isolates from the meat of wild or domestically reared pigeons from Spain, to detect the presence of virulence and antibiotic resistance genes, and to carry out a phylogenetic classification of the isolates. (mdpi.com)
  • In the present study, 1,074 ExPEC isolates were classified by phylogenetic group and possession of 67 other traits, including virulence-associated genes and plasmid replicon types. (unboundmedicine.com)
  • Integrated chromosomal and plasmid sequence analyses reveal diverse modes of carbapenemase gene spread among Klebsiella pneumoniae. (ox.ac.uk)
  • In contrast, the episomal and chromosomal gene corrections were highly dependent on the ODN length and polarity. (nih.gov)
  • The virulence of this bacterium results from the 32 Y pestis chromosomal genes and two Y pestis -specific plasmids, constituting the only new genetic material acquired since its evolution from its predecessor. (medscape.com)
  • In bacteria and archaea, CRISPRs-clustered, regularly interspaced, short palindromic repeats-constitutes a recently discovered genetic interference pathway that protects cells from phages and conjugative plasmids. (rockefeller.edu)
  • The clinical isolate S. epidermidis RP62a harbors a CRISPR spacer that matches the nickase gene ( nes ) that is present in nearly all staphylococcal conjugative plasmids and prevents their spread. (rockefeller.edu)
  • Our study provides a framework for the much needed incorporation of plasmid data into genomic surveillance systems, an essential step toward a more comprehensive understanding of resistance spread. (ox.ac.uk)
  • The effect of Mg(2+) on regulation of global gene expression was evaluated using genomic microarrays in an M-type 3 strain of GAS and in an isogenic csrS mutant. (lu.se)
  • Novel prokaryotic genomic sequences can be analyzed by the self-training software tool GeneMarkS-2 (sequences longer than 50 Kb). (gatech.edu)
  • Investments in next generation sequencing (NGS) methods over the previous 5 years now allow scientists to identify the genetic variations among many different influenza virus particles in a single sample. (cdc.gov)
  • COLUMBUS, Ohio--( BUSINESS WIRE )-- Forge Biologics , a leading manufacturer of genetic medicines, announced today that eight presentations, including four oral presentations, will be delivered during the upcoming 26th Annual Meeting of the American Society of Gene and Cell Therapy (ASGCT), taking place May 16-20, 2023, in Los Angeles, California. (biospace.com)
  • Gene duplication is long recognized as a potential source of genetic innovation ( Ohno, 1970 ). (elifesciences.org)
  • gene cloning ( DNA cloning ) The production of exact copies ( clones ) of a particular gene or DNA sequence using genetic engineering techniques. (encyclopedia.com)
  • The effects of a maize knotted1 ( kn1) gene under the control of the cauliflower mosaic virus 35S promoter on genetic transformation efficiencies of six citrus genotypes were tested. (springer.com)
  • With all six genotypes of citrus tested including Eureka lemon, a cultivar difficult to be transformed, our results demonstrate that the kn1 gene may provide an effective molecular tool to enhance genetic transformation efficiencies of various citrus varieties. (springer.com)
  • The use of genetic engineering and modern techniques such as transcriptomics, proteomics and sequence analysis are included in the course. (lu.se)
  • Antimicrobial resistance occurs through different mechanisms, which include spontaneous (natural) genetic mutations and horizontal transfer of resistant genes through deoxyribonucleic acid (DNA). (who.int)
  • To control vertical transmission of plasmids, in addition to controlled-replication systems, bacterial plasmids use different maintenance strategies, such as multimer resolution systems, post-segregational killing systems (addiction modules), and partition systems. (wikipedia.org)
  • These fragments are then inserted into cloning vectors , such as bacterial plasmids or bacteriophages, which transfer the recombinant DNA to suitable host cells, such as the bacterium E. coli. (encyclopedia.com)
  • We compare all five currently sequenced Y. pestis genomes and the corresponding features in Yersinia pseudotuberculosis . (asm.org)
  • Within CRISPR sites, the repeats are separated by short spacer sequences that match phage or plasmid genomes and specify the targets of interference. (rockefeller.edu)
  • As part of global surveillance and sequencing of carbapenem-resistant Escherichia coli , we identified a sequence type 131 strain harboring bla IMP-14 within a class 1 integron, itself nested within an ∼54-kb multidrug resistance region on an epidemic IncA/C 2 plasmid. (ox.ac.uk)
  • Foram detectadas vinte cepas produtoras de ESBL, entre as quais Escherichia coli (n=9), Klebsiella pneumoniae (n=7), Klebsiella oxytoca (n=2) e Enterobacter aerogenes (n=2), que foram posteriormente analisadas quanto a suas características de transferência de resistência, perfil plasmidial e natureza dos genes de resistência. (scielo.br)
  • In this study, choline dehydrogenase ( betA ) gene encoding for glycine betaine biosynthesis in Escherichia coli isolated from salted shark ( Scoliodon sp. (scialert.net)
  • Escherichia coli JM109 and plasmid pTZ57R/T (MBI Fermentas, Hanover, Maryland, USA) were used as transformation host and cloning vector, respectively. (scialert.net)
  • Pfizer and Moderna COVID-19 mRNA vaccines "are contaminated with plasmid DNA fragments which have not been removed during the current manufacturing processes," the doctor wrote. (zerohedge.com)
  • It isn't clear whether the presence of the SV40 sequence in the plasmid DNA of mRNA vaccines was identified by Pfizer when the company applied for approval from the FDA. (zerohedge.com)
  • We demonstrate that relatively short single-stranded oligodeoxynucleotides, 25-61 bases homologous to the target sequence except for a single mismatch to the targeted base, are capable of correcting a single point mutation (G to A) in the mutant beta-galactosidase gene, in nuclear extracts, episome, and chromosome of mammalian cells, with correction rates of approximately 0.05%, 1% and 0.1%, respectively. (nih.gov)
  • By contrast, plasmids are usually excluded or analyzed with low-resolution techniques, despite being the primary vectors of antibiotic resistance genes across many key pathogens. (ox.ac.uk)
  • Yersinia pestis has three sub species in which only two have been sequenced, strain KIM and strain CO92. (kenyon.edu)
  • Gene ID: 5925, OMIM 614041 ) gene, which encodes a tumor suppressor protein. (molvis.org)
  • One of the most important functions of the tumor suppressor p53 protein is its sequence-specific binding to DNA. (nih.gov)
  • In an Aug. 8, 2019, patent filed by Moderna regarding RNA vaccines, the company states that the direct injection of naked plasmid DNA into a living host comes with "potential problems, including the possibility of insertional mutagenesis, which could lead to the activation of oncogenes or the inhibition of tumor suppressor genes. (zerohedge.com)
  • Pathogenic Agrobacterium carry a large plasmid, referred to as the Tumor Inducing or Ti plasmid, which is required for disease transmission. (igem.org)
  • Finally, a subcutaneously C6 xenograft tumor model was established in the nude mice to test the in vitro efficacy of TK/GCV gene therapy. (hindawi.com)
  • Herpes simplex virus type 1 (HSV-1) thymidine kinase (TK) in combination with ganciclovir (GCV) has been shown as one of the most promising suicide gene systems for tumor treatment in laboratory studies and is currently being tested in several clinical trials. (hindawi.com)
  • Several PET molecular imaging agents could be used for monitoring tumor responses to prodrug activation in gene therapy. (hindawi.com)
  • This revealed three contrasting modes of dissemination used by carbapenemase genes, which confer resistance to last-line carbapenems. (ox.ac.uk)
  • ABSTRACT The bla IMP-14 carbapenem resistance gene has largely previously been observed in Pseudomonas aeruginosa and Acinetobacter spp. (ox.ac.uk)
  • In addition, investigators identified multiple antimicrobial resistance genes and mutations in most Campylobacter bacteria linked to this outbreak, indicating they were resistant to many first-line antibiotics used to treat Campylobacter infections. (cdc.gov)
  • Expression of ESBL is often associated with multidrug resistance and dissemination by resistance plasmids. (scielo.br)
  • One of these cassettes was used to express a gene of interest and the second was used to express an antibiotic resistance gene for the selection of positive transformation events. (igem.org)
  • This reservoir of resistance genes can be shared with other microbes in the gut but also holds promise for future diagnostics and treatments. (cdc.gov)
  • Polymerase chain reaction (PCR) assays and sequencing was used to determine the presence of β-lactamase encoding genes (bla) including bla NDM-1 and plasmid-mediated quinolone and aminoglycoside resistance determinants. (who.int)
  • This report presents the status of AMR in Africa by analysing the main types of resistance and the underlying genes where possible. (who.int)
  • We observed CsrS-dependent regulation of 72 of the 73 genes whose expression changed in response to elevated extracellular Mg(2+) in wild-type bacteria, a result that identifies CsrS as the principal, if not exclusive, sensor for extracellular Mg(2+) in GAS. (lu.se)
  • A plasmid is a strand of circular DNA that is common to bacteria and specific parasites. (zerohedge.com)
  • or the transmembrane protein gene cassettes of some pathogenic bacteria ( Santoyo and Romero, 2005 ), gene conversion can also generate sequence diversity. (elifesciences.org)
  • Many of the genes have been acquired from other bacteria and viruses. (kenyon.edu)
  • human insulin, for example, is now produced by bacteria containing the cloned insulin gene. (encyclopedia.com)
  • The realization of the vastness of bacterial diversity and the development of novel experimental techniques have propelled molecular microbiology into a new era of investigation This course provides insights into how genes are organized and regulated in bacteria and in their viruses. (lu.se)
  • In fact, as reported by Drancourt et al (2004), genotyping performed on bacteria derived from the remains of plague victims of the first two epidemics revealed sequences similar to that of Orientalis. (medscape.com)
  • After ingestion of infected blood, the bacteria survive in the midgut of the flea owing to a plasmid-encoded phospholipase D that protects them from digestive juices. (medscape.com)
  • Partition systems involve three elements, organized in an auto-regulated operon: A centromere-like DNA site Centromere binding proteins (CBP) The motor protein The centromere-like DNA site is required in cis for plasmid stability. (wikipedia.org)
  • ParA proteins from different plasmids and bacterial species show 25 to 30% of sequence identity to the protein ParA of the plasmid P1. (wikipedia.org)
  • Amino-acid sequence identity can go down to 21% for TubZ proteins. (wikipedia.org)
  • The complete sequences of 50 new cDNA clones which code for large proteins. (nih.gov)
  • Genes are small blocks of DNA that tell the cell which proteins it should create. (wikibooks.org)
  • The betA gene sequence reported in this study contains several base substitutions with that of reported sequences in GenBank, resulting in the altered amino acid sequences of the translated proteins. (scialert.net)
  • Moreover, the sequence analysis of choline dehydrogenase from our isolate shows several base substitutions with that of reported sequences in GenBank, resulting in the altered amino acid sequences of the translated proteins. (scialert.net)
  • The course comprises molecular biology of genes, proteins, multicomponent protein complexes, and other biomolecules that provide structure and perform the organism's functions. (lu.se)
  • Metagenomic sequences and individual short sequences (sequences MetaGeneMark . (gatech.edu)
  • Examples of clinical application of the plasmids include metagenomic sequencing in outbreak tracing and personalized medicine, such as identifying drug-microbiome interactions to improve patient outcomes ( Anthony et al. ). (cdc.gov)
  • Further, verification of mutation incorporation can be difficult when the insertion site sequence lacks convenient restriction sites for analysis. (genengnews.com)
  • First, bla OXA-48-like genes have spread primarily via the single epidemic pOXA-48-like plasmid, which emerged recently in clinical settings and spread rapidly to numerous lineages. (ox.ac.uk)
  • EcoRI restriction enzyme analyses of R plasmids were used in the detection of epidemic plasmids. (scielo.br)
  • Plasmids containing this gene, or a homologous gene. (addgene.org)
  • Following duplication events, the resulting stretches of homologous sequence can promote recombination between gene copies. (elifesciences.org)
  • The transcriptional profile of 11 fungal genes related to pathogenicity and virulence were evaluated by reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). (frontiersin.org)
  • TEM- and SHV- genes were analyzed by polymerase chain reaction (PCR) and hybridization using specific probes. (scielo.br)
  • The DNA template must be linear and contain the T7 RNA Polymerase Promoter with the correct orientation in relation to the target sequence to be transcribed, followed by an AG initiation sequence. (neb.com)
  • You can receive an email alert when new materials related to this gene are available at Addgene. (addgene.org)
  • Addgene is a nonprofit plasmid repository. (addgene.org)
  • Plasmid X from AddGene, part Y from the iGEM registry, etc. (openwetware.org)
  • Offerings include process and analytical development, plasmid DNA manufacturing, viral vector manufacturing, final fill, as well as regulatory consulting support to accelerate gene therapy programs from preclinical through clinical and commercial stage manufacturing. (biospace.com)
  • The 5´ end of the first amplicon and the 3´ end of the second amplicon overlap with the vector sequence, which has been digested with NcoI and XhoI at 37°C for 2 hours. (genengnews.com)
  • Clone the gene into the known vector. (bionexus.net)
  • The experienced staff at Bionexus's Gene Sysnthesis Services department will construct your gene in 2 weeks (1kb) and provide you with the sequencing documentation and the lyophilized gene in a standard vector. (bionexus.net)
  • Our results demonstrate that expression of the kn1 gene enhances transformation efficiencies from 3 to 15 fold compared to a control vector, 3-11 fold relative to the highest transformation efficiencies reported for these citrus genotypes. (springer.com)
  • A positive signal indicates an intact left IRT-rep gene junction has been detected and amplified, representing the maximum possible rcAAV contamination level present in the rAAV vector sample being analyzed. (nxtbook.com)
  • By introducing multiple complementary mutations in the primers at the overlap region, the Gibson Assembly Master Mix forms a single, covalently bonded DNA molecule that contains the desired mutations and can be directly transformed into competent cells and screened or sequenced. (genengnews.com)
  • In this experiment, multiple primers were designed to incorporate three mutations within the gene. (genengnews.com)
  • In this example, multiple mutations are introduced into the lacZ gene through overlapping primers followed by PCR. (genengnews.com)
  • Existing techniques for SDM, such as whole plasmid SDM, while effective, are time consuming and prone to off-target mutation incorporation. (genengnews.com)
  • Here we describe the use of New England Biolabs' Gibson Assembly Master Mix in two different mutagenesis experiments: first, multiple mutations of the lacZ gene ( Figure 1 ) and second, the mutation of 5 of the 6 nucleotides at position 174-179 of eGFP from CTGACC to TTCTAT in order to change the amino acid sequence from LeuThr to PheTyr ( Figure 2 ). (genengnews.com)
  • The reason for the observed defective assembly in E. coli is probably not some unidentified mutation in the plasmid DNA because the same plasmid preparation complemented a B. subtilis sdhCAB deletion strain [7]. (lu.se)
  • Poxvirus adaptation can involve combinations of recombination-driven gene copy number variation and beneficial single nucleotide variants (SNVs) at the same loci. (elifesciences.org)
  • In order to expand our understanding of how recombination might influence virus variation during the course of adaptation, we focused on large DNA viruses, in which rapidly evolving populations can simultaneously harbor both adaptive gene copy number variation and beneficial single nucleotide variants (SNVs) at the same locus. (elifesciences.org)
  • These nucleic acid polymers encode for the all of the materials an organism needs to live in the form of genes. (wikibooks.org)
  • These plasmids along with a pathogenicity island called HPI, create a protein that causes the pathogenicity of the organism. (kenyon.edu)
  • The ease of NDM-1 plasmid transmissibility may help their dissemination among the Enterobacteriaceae. (who.int)
  • 1 The ease of β-lactamase encoding genes ( bla NDM-1 ) dissemination has become apparent with the worldwide detection of NDM-1 producers. (who.int)
  • Spacer sequences are transcribed into CRISPR RNAs (crRNAs)-small RNAs that, through base-pairing interactions with the target sequence, guide Cas nucleases to the invasive nucleic acid to cleave it. (rockefeller.edu)
  • Strain CO92 also consists of 4,012 protein-coding genes and 150 pseudogenes. (kenyon.edu)
  • Quantification of cytosolic plasmid DNA degradation using high-throughput sequencing: implications for gene delivery. (genomeweb.com)
  • Plasmid transfer assays were performed using broth mating techniques. (scielo.br)
  • You can view Archer assays alongside IDT's xGen™ NGS portfolio to find the best next generation sequencing solution for your lab. (idtdna.com)
  • The experts at Bionexus (over 9 years experience), will construct your gene and sequence both strands to ensure that you get no mutants. (bionexus.net)
  • Sequence both strands to verify the integrity of the synthetic gene sequence. (bionexus.net)
  • Using a competition assay on agarose gels we found that the p53 consensus sequences in longer DNA fragments are better targets than the same sequences in shorter DNAs. (nih.gov)
  • Gibson Assembly™, developed by Daniel Gibson and his colleagues at the J. Craig Venter Institute, is a rapid and reliable method for the assembly of DNA fragments in a single-tube, isothermal reaction without reliance upon the presence of restriction sites within the target sequence. (genengnews.com)
  • The DNA containing the target gene(s) is split into fragments using restriction enzymes . (encyclopedia.com)
  • All identified mutations occurred at highly conserved sequences, not only in the FGFR family of molecules, but also throughout evolution and clustered in the immunoglobulin-like loop-III domain, highlighting the functional importance of this domain. (aacrjournals.org)
  • Site-directed mutagenesis (SDM) is a commonly used technique for introducing mutations into a gene of interest. (genengnews.com)
  • This system, referred to as the binary transformation system, was composed of an Agrobacterium strain containing a Ti plasmid in which the T-DNA was deleted and a second plasmid, which carries the LB and RB sequences flanking two plant gene expression cassettes. (igem.org)
  • Strain CO92 has 4,653,728 base pairs and contains three plasmids (pMT1, pCD1 and pPCP1) of 96.2 kilobases (kb), 70.3 kb and 9.6 kb. (kenyon.edu)
  • Strain KIM also carries these plasmids. (kenyon.edu)
  • The maize homeobox gene knotted1 ( kn1 ) encodes an AP2/ERF transcription factor (Hake et al. (springer.com)
  • Second, bla VIM and bla NDM genes have spread via transient associations of many diverse plasmids with numerous lineages. (ox.ac.uk)
  • Targeted sequencing for gene discovery and quantification using RNA CaptureSeq. (genomeweb.com)
  • dual-labeled hybridizable probe for detection and quantification of amplified DNA junction sequence. (nxtbook.com)
  • 7,8 ] and plasmid pX02 (96.2 kb) which cate plates of PLET agar (a selective media codes for the capsule [ 9 ]. (who.int)
  • These samples were then processed to determine S. aureus colonization status and to profile the nasal microbiota using 16S rRNA gene-based methods. (bvsalud.org)
  • As was conducted using traditional microbio- many colonies as required for finding the logical methods and with commercial anti- isolate carrying these particular genes were sera if necessary. (who.int)
  • Each plasmid has its independent replication system which controls the number of copies of the plasmid in a cell. (wikipedia.org)
  • Generally, each molecule of plasmid diffuses randomly, so the probability of having a plasmid-less daughter cell is 21−N, where N is the number of copies. (wikipedia.org)
  • For instance, if there are 2 copies of a plasmid in a cell, there is 50% chance of having one plasmid-less daughter cell. (wikipedia.org)
  • Plasmid copies are paired around a centromere-like site and then separated in the two daughter cells. (wikipedia.org)
  • Send the hard copies of sequencing data including the chromatograms. (bionexus.net)
  • GeneMark-EP+ integrates into GeneMark-ES information on cross-species protein sequences. (gatech.edu)
  • investigated the fungus at the level of gene expression, and performed proteome-level studies ( Yajima and Kav, 2006 ). (frontiersin.org)
  • A comprehensive understanding of gene expression in human RB is essential for the development of safe and effective new therapies. (molvis.org)
  • We reviewed published microarray and RNA sequencing studies in which gene expression profiles were compared between human RB and normal retina tissues. (molvis.org)
  • We investigated the expression of genes of interest using quantitative reverse transcription PCR. (molvis.org)
  • The regulatory elements required for this cell-specific gene expression are likely located within its proximal promoter. (molvis.org)
  • The terminator strengths were calculated as described previously 48 by comparing the expression of two fluorescent reporters, one placed before and one after the HTBS part (for their plasmid map, see Supplementary Fig. 14 ). (nature.com)
  • Optimize Codon Usage : Using the host's preferred codons for amino acids has been shown to dramatically improve gene expression. (bionexus.net)
  • It does not indicate whether the DNA sequence is infectious or capable of helper-virus assisted replication. (nxtbook.com)
  • Forge Biologics is a hybrid gene therapy contract manufacturing and clinical-stage therapeutics development company. (biospace.com)
  • Although the combination of herpes simplex virus type 1 (HSV-1) thymidine kinase (TK) with ganciclovir (GCV) has been shown as a promising suicide gene treatment strategy for glioma, the almost immunodepressive dose of GCV required for its adequate in vivo efficacy has hampered its further clinical application. (hindawi.com)
  • Dr. Malone said that prior FDA guidance on DNA vaccine technology pointed to the presence of "highly active regulatory sequences as being of particular concern due to potential insertional mutagenesis (integration). (zerohedge.com)
  • Gram-negative colonies were isolated and and incubated for 24 hours at 37 °C. The tested for the presence of sequences which detection of Shigella and Salmonella spp. (who.int)
  • We performed experimental evolution with vaccinia virus populations harboring a SNV in a gene actively undergoing copy number amplification. (elifesciences.org)
  • Bionexus will construct any gene the way you want it, from a few hundred base pairs to thousands of base pairs. (bionexus.net)
  • Using proprietary gene synthesis software to design and construct the entire gene. (bionexus.net)
  • How would you construct your DNA sequences in lab? (openwetware.org)
  • Second, you need a file showing the full DNA sequence of the construct. (openwetware.org)
  • The kn1 gene construct was used to transform 'Pineapple', 'Hamlin', 'Sucarri' and 'Valencia' sweet orange [ Citrus sinensis (L.) Osbeck], 'Carrizo' Citrange [ Citrus sinensis (L.) Osbeck × Poncirus trifoliate (L.) Raf. (springer.com)
  • Fourteen plasmid profiles (A, B1, B2, C1, and C2 to L) were obtained with EcoRI restriction enzyme analysis. (scielo.br)
  • We also demonstrated that the −657 to +411 DCT promoter fragment efficiently directs RB cell-specific transcription of the luciferase reporter gene in cell lines. (molvis.org)
  • This plasmid contains the mouse calcium/calmodulin-dependent kinase II alpha subunit promoter. (addgene.org)
  • By using a DNA template with a T7 promoter sequence followed by an AG initiation sequence and an encoded poly(A) tail, mRNAs can be transcribed with a 5´-m7G Cap-1 structure that is polyadenylated, translationally competent and able to evade the cellular innate immune response. (neb.com)
  • In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-mCherry plasmid DNA. (addgene.org)
  • The amino-acid sequence identity can go down to 15% between ParM and other actin-like ATPase. (wikipedia.org)