Genes sequence analysis dna. Medical search. Frequent questions

Previous studies of DNA methylation suggest epigenetic alterations may also be important, but an integrated genome-wide analysis of genetic and epigenetic alterations in ALL has not been performed. (lu.se)
We analyzed 137 B-lineage and 30 T-lineage childhood ALL cases using microarray analysis of DNA copy number alterations and gene expression, and genome-wide cytosine methylation profiling using the HpaII tiny fragment enrichment by ligation-mediated PCR (HELP) assay. (lu.se)
Genome analysis of South American Elymus (Triticeae) and Leymus (Triticeae) species based on variation in repeated nucleotide sequences. (ou.edu)
The genome origin of tetraploid species of Leymus (Poaceae: Triticeae) inferred from variation in repeated nucleotide sequences. (ou.edu)
Recent advances in nucleic acid sequencing now permit rapid and genome-scale analysis of genetic variation and transcription, enabling population-scale studies of human biology, disease, and diverse organisms. (biorxiv.org)
PG2 integrates genome and transcriptome sequencing to incorporate protein variants containing amino acid substitutions, insertions, and deletions, as well as non-canonical reading frames, exons, and other variants caused by genomic and transcriptomic variation. (biorxiv.org)
The genome of Aspergillus oryzae , a fungus important for the production of traditional fermented foods and beverages in Japan, has been sequenced. (nature.com)
Here we show that the 37-megabase (Mb) genome of A. oryzae contains 12,074 genes and is expanded by 7-9 Mb in comparison with the genomes of Aspergillus nidulans 5 and Aspergillus fumigatus 6 . (nature.com)
Sequencing of the A. oryzae genome was accomplished using the whole-genome shotgun (WGS) approach. (nature.com)
The 37-Mb genome was predicted to contain a total of 12,074 genes encoding proteins with a length greater than 100 amino acid residues (see Methods). (nature.com)
Syntenic analysis of the three aspergilli revealed the presence of syntenic blocks and A. oryzae -specific blocks of sequence (lacking synteny with the two other aspergilli) in a mosaic manner throughout the A. oryzae genome ( Fig. 1 ). (nature.com)
Phylogenetic analysis of the three aspergilli using the whole-genome data showed that A. nidulans branched off earlier than A. oryzae and A. fumigatus 5 . (nature.com)
Thus, the increase in genome size seems to be due to an A. oryzae lineage-specific acquisition of sequence, rather than loss of sequence in A. nidulans and A. fumigatus . (nature.com)
This suggests that the genome size differences are largely due to sequence acquisition in A. oryzae . (nature.com)
Whole-transcriptome sequencing (RNA-seq) technology has emerged as a revolutionary platform for genome-wide quantification of mRNA transcripts. (biomedcentral.com)
We performed a genome-wide association study (GWAS) and exome-sequencing analysis to identify common and rare genetic variants associated with plasma myeloperoxidase (MPO)-DNA complex levels, a biomarker for NETs, in the population-based Rotterdam Study cohort. (frontiersin.org)
Genome-wide association studies (GWAS) and exome-sequencing analysis have been successfully implemented as approaches to identify genetic variants associated with disease susceptibility. (frontiersin.org)
A previous study showed that unlike X. laevis, X. borealis contains a predominant or major histone gene cluster, which contains 70% of the 80-90 copies of the H4 genes in the genome (Turner & Woodland 1983 (Nucl. (bl.uk)
This species serves as an example that highlights the paramount importance of prioritizing endangered species in genome sequencing projects due to the race against time, which necessitates the comprehensive exploration and characterization of their genomic resources before their populations face extinction. (bvsalud.org)

However, most proteomic studies rely on consensus databases to match spectra to peptide and proteins sequences, and thus remain limited to the analysis of canonical protein sequences. (biorxiv.org)
Bioinformatic analyses of DNA- and protein sequences. (lu.se)

Most WFDC genes are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. (nih.gov)
A locus on human chromosome 20 contains several genes expressing protease inhibitor domains with homology to whey acidic protein. (nih.gov)
This gene is a member of the protocadherin beta gene cluster, one of three related gene clusters tandemly linked on chromosome five. (nih.gov)
Distribution and expression of the genes on chromosome 1. (nature.com)
Chromosome 18 spans about 78 million DNA building blocks (base pairs) and represents approximately 2.5 percent of the total DNA in cells. (medlineplus.gov)
Identifying genes on each chromosome is an active area of genetic research. (medlineplus.gov)
Because researchers use different approaches to predict the number of genes on each chromosome, the estimated number of genes varies. (medlineplus.gov)
Chromosome 18 likely contains 200 to 300 genes that provide instructions for making proteins. (medlineplus.gov)
The signs and symptoms of distal 18q deletion syndrome are thought to be related to the loss of multiple genes from this part of the long arm of chromosome 18. (medlineplus.gov)

10 Similarly, in recent studies, at least one third of genes in diverse organisms can exhibit alternative transcription, leading to the production of N-terminally extended proteins or alternative reading frames. (biorxiv.org)
11 And human diseases, including cancer in particular, tend to be defined by the presence of proteins with altered and pathogenic sequences. (biorxiv.org)
Biochips are playing a key role in clinical diagnosis i.e. they help in diagnosis of disease even before the symptoms are observed Biochips which can identify the presence of different proteins, Nucleic acids, different bacteria etc. in a single analysis help to diagnose the disease. (medgadget.com)
NETs are released as a result of a unique form of cell death, where DNA coated with histones and anti-microbial proteins, such as neutrophil elastase and myeloperoxidase (MPO), form a web-like structure ( 1 ). (frontiersin.org)

Finally, we identified aberrant methylation in multiple genes also targeted by recurring DNA copy number alterations in ALL, suggesting that these genes are inactivated far more frequently than suggested by structural genomic analyses alone. (lu.se)
Subsequent genomic sequence analysis of multiple genes including elongation factor 3, a component of fungi protein synthesis not found in protozoa, further supported this notion. (medscape.com)

It incorporates new DNA sequence data alignment algorithms with SNP and methylation analysis for next-generation DNA sequence data. (genecodes.com)
We found that the different genetic subtypes of ALL are characterized by distinct DNA methylation signatures that exhibit significant. (lu.se)
The role of DNA methylation and its interaction with gene expression and transcriptome plasticity is poorly understood, and current insight comes mainly from studies in very few model plant species. (nature.com)
Here, we study gene body DNA methylation (gbM) and gene expression patterns in ecotypes from contrasting thermal environments of two marine plants with contrasting life history strategies in order to explore the potential role epigenetic mechanisms could play in gene plasticity and responsiveness to heat stress. (nature.com)
In silico transcriptome analysis of CpG O/E ratios suggested that the bulk of Posidonia oceanica and Cymodocea nodosa genes possess high levels of intragenic methylation. (nature.com)
We also observed a correlation between gbM and gene expression flexibility: genes with low DNA methylation tend to show flexible gene expression and plasticity under changing conditions. (nature.com)
Furthermore, the empirical determination of global DNA methylation (5-mC) showed patterns of intra and inter-specific divergence that suggests a link between methylation level and the plants' latitude of origin and life history. (nature.com)
Gene body DNA methylation (gbM) refers to genes with enriched mCG within the transcribed regions and depletion at the transcriptional start and termination sites 16 , 17 . (nature.com)
Although the causes and potential functional consequences of gbM for gene expression are not fully understood 22 , genic CG methylation was reported to be associated with gene expression in both plants and animals 17 . (nature.com)
We have undertaken studies investigation aberrant DNA methylation resulting in changes in the expression of cancer-related genes as a possible epigenetic mechanism for Cd carcinogenesis. (cdc.gov)
Genomic DNA isolated from BALB/c-3T3 cells morphologically transformed with Cd was restriction digested with Mse1 (methylation non-sensitive) alone or with MSe1 and BstU1 (methylation sensitive). (cdc.gov)
The resulting DNA was analyzed for differential methylation using a PCR-based technique - Methylation Sensitive Restriction Fingerprinting (MSRF). (cdc.gov)
this metabolite can modify DNA methylation of normal neural and glial progenitor cells causing them to produce neoplastic glioma cells. (msdmanuals.com)

Using DNA sequencing followed by extensive bioinformatics analyses, researchers identified 34 mutations in 17 CH-driver genes. (spaceref.com)

In the 1980s, biochemical analysis of the nucleic acid composition of Pneumocystis rRNA and mitochondrial DNA identified the organism as a unicellular fungus rather than a protozoan. (medscape.com)
The results showed that those 2 isolates were multi-drug resistant and the DNA sequencing analysis showed that the alignment of nucleic acid of DNA in isolates of mycobacteria other than M. tuberculosis was different from that of M. tuberculosis complex. (who.int)

Although we cannot discern whether gbM regulates gene expression or vice versa, or if other molecular mechanisms play a role in facilitating transcriptome responsiveness, our findings point to the existence of a relationship between gene responsiveness and gbM patterns in marine plants. (nature.com)
Whole transcriptome sequencing (RNA-seq) represents a powerful approach for whole transcriptome gene expression analysis. (biomedcentral.com)
The Ion AmpliSeq™ Transcriptome Human Gene Expression Kit (AmpliSeq) was recently introduced by Life Technologies as a whole-transcriptome, targeted gene quantification kit to overcome these limitations of RNA-seq. (biomedcentral.com)
Transcriptome analysis has enhanced our understanding of the molecular constituents of cells and tissues. (biomedcentral.com)
As one of the most widely used tools for transcriptome profiling, quantification of differential gene expression has played a pivotal role in the identification of pathway and gene-network interactions [ 1 - 3 ]. (biomedcentral.com)

Infant gut commensals risk transferring multidrug-resistant antibiotic resistance genes (ARGs) to pathogenic bacteria. (biomedcentral.com)

However, recent studies in many organisms and in humans have revealed significant protein sequence variation due to the presence of somatically acquired genetic variants, alternative transcription, and mRNA splicing, which are not necessarily annotated in reference databases. (biorxiv.org)
b) Number of somatic nonsynonymous single nucleotide variants (SNVs) in CHIP-driver genes harbored per subject. (spaceref.com)
We additionally performed exome-sequencing analysis in 960 individuals to investigate rare variants in candidate genes. (frontiersin.org)
We performed additional in silico analyses to identify more evidence for the associated variants and genes in relation to the plasma MPO-DNA complex levels. (frontiersin.org)

Methods for analysis of gene expression: microarray, hybridisation, promoter analyses. (lu.se)

Characteristics of Clonal Hematopoiesis (CH) Mutations - a) We identified somatic mutations in known clonal hematopoiesis of indeterminate potential (CHIP) driver genes using peripheral blood mononuclear cells isolated from 14 astronauts who flew short space Shuttle missions lasting a median of 12 days between 1998-2001. (spaceref.com)
They discovered DNA mutations, known as somatic mutations, in the blood-forming system (hematopoietic stem cells) in all 14 astronauts studied. (spaceref.com)
The most frequent mutations occurred in TP53, a gene that produces a tumor-suppressing protein, and DNMT3A, one of the most frequently mutated genes in acute myeloid leukemia. (spaceref.com)
However, the frequency of the somatic mutations in the genes that the researchers assessed was less than two percent, the technical threshold for somatic mutations in hematopoietic stem cells to be considered clonal hematopoiesis of indeterminate potential (CHIP). (spaceref.com)
DNA sequence analyses and reporter gene assays were performed in order to identify candidate gene mutations. (bmj.com)
Mutations in the genes encoding the CNGA3 and CNGB3 subunits of the cyclic nucleotide-gated (CNG) channel of cone photoreceptors have been associated with autosomal recessive achromatopsia. (jneurosci.org)
Segregation analysis and exclusion of the mutations T224R and T369S in 100 control subjects (200 chromosomes) was performed by restriction fragment length polymorphism (RFLP) analysis of PCR-amplified fragments, including all available family members. (jneurosci.org)
Secondary glioblastomas typically have mutations in the IDH1 or IDH2 genes. (msdmanuals.com)

The conformational analysis was performed utilizing FT-IR matrix isolation technique and theoretical methods such as density functional theory and ab initio calculations. (researchgate.net)
Deep sequencing followed by abundancy ranking pinpointed six top hit genes potentially associated with either improved or impaired endodermal differentiation, which were selected for functional validation in CRISPR-Cas9 mediated knockout (KO) lines. (mdpi.com)
The advent of new methods to obtain large-scale surveys of various mathematical structures, such as systems of differen- gene expression in which transcript levels can be determined tial equations, graphical networks, stochastic functional re- for thousands of genes simultaneously has facilitated the ex- lations, and simulation models. (lu.se)
eling require a broad range of signal processing approaches, Two salient goals of functional genomics are to screen for including signal representation relevant to transcription and key genes and gene combinations that explain specific cel- system modeling using nonlinear dynamical systems. (lu.se)
Microinjection of the major cluster clones in to Xenopus oocytes, confirmed that these genes were functional. (bl.uk)
Microinjection analysis again showed the genes were functional. (bl.uk)

This study aimed to identify polymorphic nucleotides, changes in amino acid components, and species of goats based on GenBank Leptin DNA sequence data. (unair.ac.id)
A study of 28 Elymus species using repetitive DNA sequences. (ou.edu)
Therefore, in this study, two regions of the gene matK of 11 samples of Solanum procumbens were collected from the provinces of Ha Noi, Quang Ngai, Phu Yen, Gia Lai, Lam Dong, Ba Ria - Vung Tau, Long An in Vietnam has been conducted by order and analyzing species by DNA Barcode method. (nih.gov)
Previous work has shown that transcriptional activation of herpes simplex virus type 1 (HSV-1) immediate early genes is mediated by a protein species (Vmw65) present in the tegument of infecting virions. (gla.ac.uk)
Based on plastid encoded DNA sequences from psb A and rbc L markers, these species belong in the subfamily Chamberlainoideae. (degruyter.com)
The DNA sequences, supported by the morpho-anatomical character of tetrasporangial conceptacle roof development, placed all three species in the genus Chamberlainium and not Pneophyllum , the only other genus in Chamberlainoideae. (degruyter.com)

In this first GWAS and exome-sequencing analysis of NETs levels, we found several genes that were associated with NETs. (frontiersin.org)

DNA sample stability is dependent on the methodology used for the isolation. (ddccliniclab.org)
To assess the performance of this new methodology, we performed a comprehensive comparison of AmpliSeq with RNA-seq using two well-established next-generation sequencing platforms (Illumina HiSeq and Ion Torrent Proton). (biomedcentral.com)

These results provided the first report for further studies on the genetic diversity of leptin genes in different local goat breeds. (unair.ac.id)
Carrier screening is a term used to describe genetic testing that is performed on an individual who does not have any overt phenotype for a genetic disorder but may have one variant allele within a gene(s) associated with a diagnosis. (acog.org)
In a recent experiment, a team of researchers from the University of Exeter conducted an analysis of worms on the ISS and noted "subtle changes" in their genetic makeup. (universetoday.com)
To further elucidate how the genetic variability in the OPRM1 gene contributes to opioid efficacy and side-effects, further studies are required in different races or ethnicities and in different types of pain or surgery with sufficient sample sizes ( 18 ). (spandidos-publications.com)
Gene technology: basic and applied molecular genetic methods. (lu.se)
When indicated, genetic testing for a germline mutation should be done on the most informative candidate(s) identified through the family history evaluation and/or tumor analysis to confirm a diagnosis and allow for predictive testing of at-risk relatives. (medscape.com)
Tests include immunohistochemistry (IHC) testing, microsatellite instability (MSI) testing (usually used as a prescreening test), and DNA analysis (considered unnecessary, expensive, and time-consuming) (see Genetic Testing ). (medscape.com)

Genomic DNA from the patient was screened for chromosomal imbalances by array-based comparative genomic hybridisation. (bmj.com)
The picture of histone gene cluster structure in X. borealis that emerges from these studies, is compared at the nucleotide, gene, and chromosomal levels with X. laevis. (bl.uk)

Gene linkage testing and direct mutation analysis/DNA sequencing . (pkdcure.org)

Phylogenetic analysis of the cadherin superfamily allows identification of six major subfamilies besides several solitary members. (nih.gov)
Instead, it relies on the identification of certain "markers" in the DNA of several members of a family in which PKD has been diagnosed. (pkdcure.org)
Identification of Gene Products Encoded by the Latency-Related Gene of" by Ashfaque Hossain, L. M. Schang et al. (unl.edu)
In addition, to optimise the use of wood/wood product identification (taxonomic identity or geographic origin) in support of law enforcement, the guide anticipates upcoming developments to combine different timber identification methods such as wood anatomy, DNA-based methods, stable isotopes, DART TOFMS and NIRS. (researchgate.net)
Methods for gene identification and analysis of gene structure: cloning, PCR, restriction mapping, in situ hybridisation, DNA sequencing. (lu.se)

Further analyses of gut microbiota using next-generation sequencing (NGS) of the 16S rRNA gene showed that the pattern of gut microbial composition was clearly altered following 8 weeks of TWK10 administration. (frontiersin.org)
However, the gut microbiota is a reservoir for antibiotic resistance genes (ARGs). (biomedcentral.com)

This technique enables the sequencing of all RNA molecules in a high-throughput manner. (biomedcentral.com)
Therefore it is not surpris- pansion of biological understanding from the analysis of in- ing that the advent of high-throughput genomic and pro- dividual genes to the analysis of systems of genes (and pro- teomic technologies is drawing a growing interest from the teins). (lu.se)
So far, we have not ventured into ultra high-throughput sequencing, for two main reasons. (genomeweb.com)

Patient A had no identified risk factor for HIV infection and was infected with a strain of HIV closely related to that of her dentist as determined by viral DNA sequencing. (cdc.gov)
Strategies for gene therapy and production of medicines via genetically-modified organisms (expression vectors and viral vectors). (lu.se)

Additionally, for genes with high abundance, AmpliSeq outperforms the two RNA-seq methods. (biomedcentral.com)

The leptin DNA sequence was aligned with Bioedit to locate SNPs and amino acid changes. (unair.ac.id)
Based on the DNA sequencing results of leptin genes in five goats, five SNPs were located in the coding sequence (CDS), SNPs g.17T/A, g.43T/A, g.74G/A, g.93C/A. and d. 386A/G. SNP was a missense mutation and a silent mutation. (unair.ac.id)
Numerous single nucleotide polymorphisms (SNPs) have been identified in the OPRM1 gene [ http://www.ncbi.nlm.nih.gov/SNP/snp_ref.cgi?locusId=4988 ]. (spandidos-publications.com)
5.0 × 10 −6 ) of SNPs annotated to four genes. (frontiersin.org)

1998). "Prediction of the coding sequences of unidentified human genes. (wikipedia.org)

We also identified an epigenetic signature common to all cases, with correlation to gene expression in 65% of these genes, suggesting that a core set of epigenetically deregulated genes is central to the initiation or maintenance of lymphoid transformation. (lu.se)
The mechanisms by which gene expression, and thus phenotypic plasticity, are influenced and regulated remain poorly understood in most organisms. (nature.com)
DNA hypermethylation and loss of expression of the P16 tumor suppressor gene in cadmium transformed BALB/C-3T3 cells. (cdc.gov)
Further studies have shown that the expression of p16 tumor suppressor gene was significantly lower in the transformed cells compared wiht the non-transformed cells. (cdc.gov)
Since DNA hypermethylation is known to result in gene silencing, it appears that the decreased expression p16 gene in the Cd transformed BALB/c-3T3 cells may be due to its hypermethylation. (cdc.gov)
Further, decreased expression of the p16 gene may in part be responsible for the Cd-induced cell transformation and tumorigenesis. (cdc.gov)
All three statistical methods demonstrate AmpliSeq as a highly accurate method for differential gene expression analysis. (biomedcentral.com)
When analyzing four closely related hiPSC-CM lines, we show that both AmpliSeq and RNA-seq capture similar global gene expression patterns consistent with known sources of variations. (biomedcentral.com)
Our study indicates that AmpliSeq excels in the limiting areas of RNA-seq for gene expression quantification analysis. (biomedcentral.com)
Thus, AmpliSeq stands as a very sensitive and cost-effective approach for very large scale gene expression analysis and mRNA marker screening with high accuracy. (biomedcentral.com)
In the past decade, microarrays and RT-qPCR have been the primary tools for analyzing gene expression changes. (biomedcentral.com)
Though accepted as the gold-standard for measuring gene expression, this method is lower throughput and not amenable to perform global expression analyses. (biomedcentral.com)
Expression of latency-related (LR) gene products is controlled by a 980-bp fragment (LR promoter). (unl.edu)
When the LR gene was inserted into a mammalian expression vector and subsequently transfected into COS-7 cells, a 41-kDa protein was detected by use of silver-stained sodium dodecyl sulfate-polyacrylamide gels and by the P2 antibody. (unl.edu)
Principles of gene expression. (lu.se)
Molecular mechanisms for regulation of gene expression at different levels: remodeling of chromatin, initiation of transcription, nuclear transport and signalling, and RNA interference. (lu.se)
Principles for regulation of gene expression through intercellular signalling. (lu.se)
How Reliable Are Gene Expression-Based and Immunohistochemical Biomarkers Assessed on a Core-Needle Biopsy? (lu.se)

Major cluster clone XbHW302 was restriction site mapped, and certain regions sequenced. (bl.uk)
Restriction site mapping and DMA sequencing of clone XbHW61 allowed the location polarity, and identity of an H3, H4, and an HI gene to be determined. (bl.uk)

At least five genes were shown to be essential for the synthesis of AHBA, five genes were determined to encode the modular type I PKS that uses AHBA as the starter unit, and 20 or more genes appear to govern modification of the polyketide-derived framework, and rifamycin resistance and export. (nih.gov)
The blocks of A. oryzae -specific sequence are enriched for genes involved in metabolism, particularly those for the synthesis of secondary metabolites. (nature.com)
Deletion of DNA sequences containing ORF 2 blocked synthesis of the 41-kDa protein in COS-7 cells. (unl.edu)
He holds an M.S. degree in computer sci- damental discipline that brings to genomics the structural ence from Stevens Institute of Technology model-based analysis and synthesis that form the basis of in 1986 and a Ph.D. degree in mathemat- ics from Rutgers University in 1974. (lu.se)

Disruption of the PKS genes at the end of rifA abolished rifamycin B production and resulted in the formation of P8/1-OG, a known shunt product of rifamycin biosynthesis, whereas disruption of the orf6 and orf9 genes, which may encode deoxysugar biosynthesis enzymes, had no apparent effect. (nih.gov)
All three genes encode key components of the phototransduction cascade in cone photoreceptors. (jneurosci.org)

abstract = "The Leptin gene is the gene that produces the leptin hormone, which is released from adipose tissue and can increase the productivity of animals. (unair.ac.id)

The company, which has about 15 employees but shares additional staff members and resources with its parent firm, currently offers a variety of genomic services, including next-generation sequencing on the Roche/454 GS FLX and GS Junior platforms. (genomeweb.com)
What kinds of next-gen sequencing services do you offer, and on what platforms? (genomeweb.com)

RT-PCR products from six patients who died from TBE between 2013 and 2017 were directly sequenced and analysed phylogenetically. (who.int)

Unlike the alpha and gamma clusters, the transcripts from these genes are made up of only one large exon, not sharing common 3' exons as expected. (nih.gov)

Gene regulation in developmental biology and the cell cycle. (lu.se)

The gene clusters demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. (nih.gov)
In this longitudinal cohort study of infants living in a region with high endemic antibacterial resistance, we demonstrate that majority of the infants harboured several antibiotic resistance genes in their gut and showed that the infant gut resistome is diverse and dynamic over the first year of life. (biomedcentral.com)
The previous experiments performed by Rubin - which were part of the Biomolecule Sequencer investigation - sought to demonstrate that DNA sequencing is feasible in an orbiting spacecraft. (universetoday.com)

This hands on course will give you additional experience in analyzing next generation sequencing data. (lu.se)

DNA fragments differentially methylated in the transformed cells compared with the non-transformed cells were indentified by MSRF and subcloned into the TA-cloning vector. (cdc.gov)
RNA-seq offers several advantages over microarrays or RT-qPCR for detecting differentially expressed genes (DEGs). (biomedcentral.com)

Several techniques based on the polymerase chain reaction (PCR) to facilitate the characterization and phylogenetic analysis of variola virus isolates were described. (who.int)
Human evidence of this is provided by molecular analysis of Pneumocystis isolates obtained from groups of patients involved in hospital outbreaks. (medscape.com)

PG2 can be integrated with current and emerging sequencing technologies, assemblers, variant callers, and mass spectral analysis algorithms, and is available open-source from https://github.com/kentsisresearchgroup/ProteomeGenerator2 . (biorxiv.org)

Rifamycin production in A. mediterranei is governed by a single gene cluster consisting of structural, resistance and export, and regulatory genes. (nih.gov)
This gene belongs to the telomeric cluster. (nih.gov)
The beta cluster contains 16 genes and 3 pseudogenes, each encoding 6 extracellular cadherin domains and a cytoplasmic tail that deviates from others in the cadherin superfamily. (nih.gov)
Analysis of these clones indicated one class containing the major cluster, and a second, minor class which appeared to be cloned at high frequency. (bl.uk)

This gene encodes a member of the WAP-type four-disulfide core (WFDC) domain family. (nih.gov)
The human mu-opioid receptor gene (OPRM1) encodes the μ -opioid receptor protein, which is the main target of the analgesic, fentanyl. (spandidos-publications.com)
Antibody P2, which is directed to sequences near the amino terminus of ORF 2, recognized a 41-kDa protein in lytically infected cells, suggesting that ORF 2 encodes a protein. (unl.edu)

Extensive DNA sequence comparisons between various Xenopus histone gene clusters were undertaken. (bl.uk)

This paper describes DNA sequence analysis and mRNA mapping of the Vmw65 gene in HSV-1 strain 17. (gla.ac.uk)
The number of sequencing reads that map to each transcript is used to infer the abundance of mRNA molecules. (biomedcentral.com)
RNA sequencing of microRNAs (miRNAs) derived from EVs (EV-miRNAs) and mRNA transcripts from blood cells was used for the construction of signaling networks reflecting endocannabinoid and miRNA communication by EVs to target immune cells. (bvsalud.org)

Specific expansion of genes for secretory hydrolytic enzymes, amino acid metabolism and amino acid/sugar uptake transporters supports the idea that A. oryzae is an ideal microorganism for fermentation. (nature.com)

Genes Dev, 2000 May 15. (nih.gov)

So we could identify interesting genes or biomarkers using microarrays, validate that using qPCR on a larger sample size, and then if it is of interest to a customer, we could go a step further and say, 'let's resequence the genes' and help them all the way towards a clinical study where the mutation status of a certain gene might be of interest. (genomeweb.com)

The Committee concluded that additional work may be needed to assess the viability of the stocks held in VECTOR, and that further molecular characterization of additional strains may be valuable in helping to identify strains from which further DNA sequences could be determined. (who.int)

The analysis of phylogenetic trees of Leptin showed that there were three breeds of goats in one branch and two breeds of goats in different branches. (unair.ac.id)

The objectives of this document are to provide considerations for the benefits (both short- and long-term), challenges, and limitations of doing an on-farm environmental investigation and resources for conducting an environmental investigation as a component of a root cause analysis (RCA) in a produce growing environment. (cdc.gov)

This method is capable of identifying those changes in the sequence that cause PKD. (pkdcure.org)
The practice of ongoing, systematic environmental monitoring and analysis is recommended because it can aid in identifying trends, patterns, or associations that can point to suspected factors which may contribute to on-farm contamination. (cdc.gov)

Paul collaborates with phycologists worldwide sequencing field-collected and type specimens of seaweeds from the 18th, 19th, and early 20th centuries, especially coralline red algae. (degruyter.com)
Results CMV DNA was detected in 94 (56.97%) of the 165 aqueous humour specimens analysed. (bmj.com)
Owing to the quantity requirement for CMV gB genotype analysis, results could be obtained from only 14 specimens. (bmj.com)

Expertise and first-class facilities provide support for researchers to achieve optimum analysis and offer skill development opportunities at the front line of blue sky and translational research techniques. (otago.ac.nz)
Researchers are working to determine how the loss of specific genes in this region contributes to the various features of the disorder. (medlineplus.gov)

For example, most human tissues in healthy individuals acquire somatic nucleotide substitutions, insertions, deletions and DNA rearrangements, leading to the production of variant protein isoforms. (biorxiv.org)

Sequences were separated on an ABI 377 DNA sequencer (Applied Biosystems), analyzed manually and independently by two experimenters, and assembled with SeqMan software (DNASTAR, Madison, WI). (jneurosci.org)

Methylated CG (mCG) is the most abundant form of mC in plant genomes and predominantly occurs in noncoding regions such as transposable elements and other repetitive DNA regions 14 , 15 . (nature.com)
Phylogenetic analysis of the genus Hordeum using repetitive DNA sequences. (ou.edu)

all infants harboured these antibiotic resistance genes at some point in the first year of life. (biomedcentral.com)
This is the first study in the region describing changes in the total antibiotic resistance gene (ARG) profile (also known as the resistome) of healthy infants over time. (biomedcentral.com)

Their current applications lie principally in the analysis of genes for defects or sequence variations. (medgadget.com)

DNA sequencing and sequence similarity analysis identified one of the aberrantlyl methylated DNA fragments as the p16 tumor suppressor gene. (cdc.gov)

Pathway analysis of the identified genes showed their involvement in cellular development, molecular transport, RNA trafficking, cell-to-cell signaling and interaction, cellular growth and proliferation. (frontiersin.org)

Molecular analysis was carried out in Trans-Disciplinary University of Health Science and Technology and Eurofins Genomics India, Bangalore. (ijpsonline.com)

First, there is miniPCR , a device which copies targeted pieces of DNA in a process known as Polymerase Chain Reaction (PCR) to create thousands of copies. (universetoday.com)
OPRM1 A118G was screened by DNA sequence analysis of polymerase chain reaction (PCR)-amplified DNA. (spandidos-publications.com)

Anatomy6

Organisms77

Diseases7

Chemicals and Drugs67

Analytical, Diagnostic and Therapeutic Techniques and Equipment22

Phenomena and Processes58

Disciplines and Occupations5

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture4

Information Science5

Health Care20

Geographicals11

Genome19

Protein sequences2

Chromosome9

Proteins4

Multiple genes2

Methylation13

Bioinformatics1

Nucleic2

Transcriptome5

Pathogenic1

Variants4

Microarray1

Mutations8

Functional6

Species6

Several genes1

Methodology2

Genetic7

Chromosomal2

Mutation analysis1

Identification5

Microbiota2

High-throughput3

Viral2

Additionally1

SNPs4

19981

Expression18

Restriction2

Synthesis4

Encode2

Abstract1

Platforms2

20171

Exons1

Regulation1

Demonstrate3

Next-generation1

Differentially2

Isolates2

Algorithms1

Cluster4

Encodes3

Clusters1

MRNA3

Metabolism1

20001

Biomarkers1

Characterization1

Phylogenetic trees1

Investigation1

Identifying2

Specimens3

Researchers2

Variant1

Sequencer1

Repetitive2

Antibiotic2

Defects1

Tumor1

Pathway1

Genomics1

Polymerase2