AntibodyEscherichiaPurificationSaccharomycesColiAntibodiesPurityCloningAffinityNucleocapsid proteinPeptidesELISAPutativeAssayMembrane proteinHumanCharacterizationMolecular weightMammalianSubunitBacterialVaccinesCatalogVivoYeastEukaryoticLocalizationVitroTranscriptHomologVectorsSubstancesReporterAssaysHomoConcentrationBindsPhenotypesTranscriptionalTypeProductsSynthesisSpike proteinCharacterisationCarbonic anhydraseCysteineEnhancesStabilityFunctionalProduction
Antibody4
- Neither did antibody to the recombinant 37,000 dalton protein react with any treponemal proteins purified by fibronectin affinity chromatography. (bmj.com)
- Finally, only sera of patients with primary syphilis possessed appreciable concentrations of antibody to recombinant P2 protein. (bmj.com)
- If you can't find the antibody you need, please check CUSABIO protein custom service . (cusabio.com)
- Various techniques have been developed for targeting cancer cells: gene therapy, monoclonal antibodies (MAbs), antibody toxin conjugates, small-molecule inhibitors, antisense molecules, and tumor vaccines. (medscape.com)
Escherichia3
- The recombinant protein in Escherichia coli lysates was labelled P2 (P2 star) to differentiate it from the comigrating adhesin protein called P2. (bmj.com)
- Recent studies have shown that it may be possible to produce recombinant lysosomal proteins with microorganisms such as Escherichia coli and Saccharomyces cerevisiae. (wikipedia.org)
- An S-peptide Fucus MT fusion protein expressed in Escherichia coli exhibited a relative molecular mass of ∼ 14 kDa. (portlandpress.com)
Purification9
- Protein production Gene expression Protein purification Host cell protein Young CL, Britton ZT, Robinson AS (May 2012). (wikipedia.org)
- We own protein expression technology based on a series of protein expression and purification technology, including protein expression technology shown by Baculovirus outer membrane proteins (OMP), and membrane protein expression technology, etc. (cusabio.com)
- The new extracted gene was sequenced and cloned in E. coli, followed by protein purification using a C-terminal His-tag. (bvsalud.org)
- A His-tag will be added to each recombinant protein to facilitate protein purification. (usda.gov)
- In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization). (antibodies-online.com)
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. (antibodies-online.com)
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. (antibodies-online.com)
- The students will have opportunity to practice several of these methods during laboratory work, including site-directed mutagenesis, recombinant protein production and purification, real-time RT-PCR. (lu.se)
- Purification and characterization of two protein kinases acting on the aquaporin SoPIP2;1. (lu.se)
Saccharomyces1
- The Thermus thermophilus xylA gene encoding xylose (glucose) isomerase was cloned and expressed in Saccharomyces cerevisiae under the control of the yeast PGK1 promoter. (lu.se)
Coli8
- Hence, an expression system performed in E. coli BL21 (DE3) using the vector pET-SUMO with a His-tag was prepared, resulting in a synthetic M gene of 654 pb to produce a recombinant M-PDCoV protein ( r M-PDCoV). (authorea.com)
- Compared with traditional protein expression systems, many processes are omitted in vitro E.coli, such as the transformation of plasmids, cell culture, collection, crushing and centrifugation, etc., which greatly improves working efficiency. (cusabio.com)
- In addition, salt resistance experiments showed that the survival of recombinant E. coli+DnaJ was 2.1 times more than control cells in 0.5 M NaCl. (bvsalud.org)
- Furthermore, the number of recombinant E. coli BL21+DnaJ colonies was 7.7 times that of the control colonies in pH 8.5. (bvsalud.org)
- Recombinant Human JNK2 protein(1-424 aa), fused to GST tag, was expressed in E. coli. (creativebiomart.net)
- Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. (antikoerper-online.de)
- An E. coli strain that carries a plasmid encoding the cloned human histone H3.3 gene, H3F3A or H3F3B. (neb.com)
- The drug substance anakinra is a recombinant protein (Interleukin-1 receptor antagonist) produced in E. coli bacteria containing an expression plasmid in which a synthetic gene coding for human IL-1Ra has been inserted.According to the Guideline on the Environmental Risk Assessment of Medicinal Products for Human Use (EMEA 2006), proteins are exempted from environmental risk assessment because they are unlikely to result in significant risk to the environment. (janusinfo.se)
Antibodies8
- The recombinant proteins will be used as antigens to produce polyclonal antibodies. (usda.gov)
- The antibodies will be used for western blots or immunostaining to detect the presence of the Hessian fly proteins in infested wheat tissues. (usda.gov)
- Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies. (antikoerper-online.de)
- Monoclonal antibodies are a type of protein used for treatment of a wide array of medical indications. (fujifilm.com)
- Within the past 2 years, investigational peptide- and recombinant protein-based serologic assays that can more easily differentiate the antibodies to HTLV-I and HTLV-II have been developed (8,9). (cdc.gov)
- Targeted molecular therapy, like therapy with monoclonal antibodies, gene therapy, and other therapies, has limited or nonexistent side effects on normal cells of the body, unlike present modalities such as surgery, chemotherapy, and radiotherapy. (medscape.com)
- however, rabbit antibodies raised against the recombinant proteins encoded by the gene of mouse bullous pemphigoid antigen 2 (BP180) are capable of inducing blisters in newborn mice in a complement-dependent manner. (medscape.com)
- 135 antibodies against 65 mainly immunoregulatory proteins, we of a multitude of different genes (8, 9). (lu.se)
Purity2
- If you are looking for recombinants with excellent quality (validated bio-activity, lower endotoxin level and high purity) to support your research, you are in the right place. (cusabio.com)
- The purity of Human ACE2 (18-615) Recombinant Protein (hFc-Tag) was determined by densitometry after SDS-PAGE of 1 μg of protein, followed by staining with Coomassie Blue. (cellsignal.com)
Cloning4
- Cloning structural genes for Treponema pallidum immunogens and characterisation of recombinant treponemal surface protein, P2 (P2 star). (bmj.com)
- Cloning, expression, and characterization of potential immunogenic recombinant porcine deltacoronavirus (PDCoV) membrane protein from a synthetic gene. (authorea.com)
- Gene cloning and characterization of a novel recombinant 40-kDa heat shock protein from Mesobacillus persicus B48. (bvsalud.org)
- The human glucosamine-6-phosphate deaminase gene: cDNA cloning and expression, genomic organization and chromosomal localization. (nih.gov)
Affinity4
- Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. (origene.com)
- The encoded protein is secreted and binds hydroxyapatite with high affinity. (origene.com)
- The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. (abcam.com)
- Affinity tags can reduce merohedral twinning of membrane protein crystals. (lu.se)
Nucleocapsid protein1
- A capture IgM EIA (non-quantitative) that incorporates a recombinant measles virus nucleocapsid protein as the antigen is used to detect measles IgM. (cdc.gov)
Peptides3
- The tyrosinase‐induced polymerization of peptides offers facile access to artificial mussel foot protein analogues. (wikipedia.org)
- The use of amino acids, proteins and peptides is not considered to have any environmental impact. (janusinfo.se)
- According to the European Medicines Agency guideline on environmental risk assessments for pharmaceuticals (EMA/CHMP/SWP/4447/00),vitamins, electrolytes, amino acids, peptides, proteins, carbohydrates, lipids proteins, vaccines and herbal medicinal products are exempted because they are unlikely to result in significant risk to the environment. (janusinfo.se)
ELISA1
- In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (rndsystems.com)
Putative4
- Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. (rndsystems.com)
- Several genes encoding putative Hessian fly effector family 31 members have been cloned previously. (usda.gov)
- A gene was identified (1229 bp) with a putative open reading frame (204 bp) encoding a 67-amino-acid protein exhibiting several characteristic features of MT proteins, including 16 cysteine residues (24%) and only one aromatic residue. (portlandpress.com)
- Although the protein sequence showed high identity with plant and invertebrate MTs, it contained a unique 'linker ' region (14 amino acid residues) between the two putative metal-binding domains which contained no cysteine residues. (portlandpress.com)
Assay1
- A dilution series of the recombinant Lucia luciferase protein can also be used to determine the linear range of the assay. (invivogen.com)
Membrane protein3
- We select from GenBank a group of 138 sequences and obtained a consensus PDCoV membrane protein (M-PDCoV) sequence of 216 a.a. (authorea.com)
- CD137 ligand (CD137L) is a type II membrane protein and part of the TNF superfamily. (fishersci.com)
- Angiotensin-Converting Enzyme 2 (ACE-2) is an integral membrane protein and a zinc metalloprotease of the ACE family, the ACE family includes somatic and germinal ACE. (prosci-inc.com)
Human19
- In this study, we showed that the human RFC (hRFC) gene can serve as a novel selectable marker for the overproduction of recombinant proteins. (aspetjournals.org)
- In many cases, recombinant human proteins have replaced the original animal-derived version used in medicine. (wikipedia.org)
- The prefix "rh" for "recombinant human" appears less and less in the literature. (wikipedia.org)
- We previously demonstrated that 350 bp of the human rod cGMP phosphodiesterase beta-subunit (beta-PDE) gene promoter are sufficient to direct high levels of gene expression in human Y-79 retinoblastoma cells in vitro. (nih.gov)
- This study demonstrates the utility of the human beta-PDE promoter for directing the expression of foreign genes to photoreceptors and suggests that the -297 to +53 beta-PDE 5' flanking region fragment may have important implications for therapeutic gene delivery to the visual cells. (nih.gov)
- The Recombinant Human Lysyl Oxidase Homolog 2 Protein is derived from NS0. (fishersci.com)
- The Recombinant Human Lysyl Oxidase Homolog 2 Protein has been validated for the following applications: Enzyme Activity. (fishersci.com)
- Binding response kinetics between Human ACE2 (18-615) Recombinant Protein (hFc-Tag) (immobilized) and SARS-CoV-2 Spike (trimeric) (16-1208) Recombinant Protein (8xHis-Tag) #65444 (in solution, at indicated concentrations). (cellsignal.com)
- Recombinant human/mouse/rat BMP-2 (355-BM) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. (novusbio.com)
- When Recombinant Human B7-H2 Fc Chimera Recombinant Human B7‑H2 Fc Chimera (Catalog # 165-B7 ) is immobilized at 0.1 µg/mL (100 µL/well), Biotinylated Recombinant Human ICOS Fc Chimera binds with an ED 50 of 1-6 ng/mL. (rndsystems.com)
- Recombinant Human EBI3(Arg21-Lys229) and p28(Phe29-Pro243) fused with His tag at C-terminal was expressed in NS0. (creativebiomart.net)
- The human EBI3 gene encodes a 229 amino acid (aa) precursor that contains a 20 aa signal peptide and 209 aa mature protein. (creativebiomart.net)
- The human p28 gene encodes a 243 aa precursor that contains a 28 aa signal sequence and 215 aa mature region. (creativebiomart.net)
- The following product was used in this experiment: Human MAP4K5 (KHS1), GST Tag Recombinant Protein from Thermo Fisher Scientific, catalog # PV3682. (thermofisher.com)
- The adeno-associated virus (AAV) is one of the most promising viral vectors for human gene therapy. (nature.com)
- Human PRB1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade. (antibodies-online.com)
- As an example of protein therapy, a completed phase I/II trial associated recombinant human collagen with wound healing and pain reduction in RDEB. (medscape.com)
- Here we describe a different method of transgene regulation by the use of the human glial ®brillary acidic protein (GFAP) promoter. (lu.se)
- The use of viral vectors to deliver foreign genes to the brain is highly logical states in the human brain (Eng et al. (lu.se)
Characterization1
- The present study reports the recognition and characterization of the gene encoding the co-chaperone DnaJ in the halophilic strain Mesobacillus persicus B48. (bvsalud.org)
Molecular weight3
- Treponemal proteins ranging in molecular weight from 37,000 daltons to 120,000 daltons were identified by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and immunoblotting of phage lysate proteins. (bmj.com)
- The mature protein has an approximate molecular weight of 24 kDa. (invivogen.com)
- Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. (novusbio.com)
Mammalian3
- As a result, recombinant lysosomal proteins are usually produced in mammalian cells. (wikipedia.org)
- A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. (antikoerper-online.de)
- The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. (antikoerper-online.de)
Subunit3
- It is composed of EBI3 (EBV-induced gene 3), a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, the 28 kDa glycoprotein that is related to the p35 chain of IL-12). (creativebiomart.net)
- Recombinant subunit protein vaccines use larger pieces of proteins from HIV. (healthline.com)
- This protein is the full-length p52 subunit and partial p100 subunit. (abcam.com)
Bacterial2
- The PEP enzyme recognizes the -10 and -35 cis -elements, similar to those found in bacterial promoters whereas the NEP enzyme recognizes the YRTA-motif, which can also be found upstream of several genes with PEP promoters indicating that these genes can be transcribed by both polymerases ( Pfannschmidt and Liere, 2005 ). (frontiersin.org)
- A construct containing the bacterial LacZ gene driven by a fragment of the beta-PDE 5' flanking region (-297 to +53) was used to generate transgenic mice. (nih.gov)
Vaccines5
- Peptide vaccines use small proteins from HIV to trigger an immune response. (healthline.com)
- Live vector vaccines use non-HIV viruses to carry HIV genes into the body to trigger an immune response. (healthline.com)
- FUJIFILM Diosynth Biotechnologies' high-tech labs build APIs for different types of vaccines, including viral vector and recombinant DNA vaccines. (fujifilm.com)
- Recombinant vaccines are genetically engineered using DNA encoding that stimulates a response in the body, activating the immune system. (fujifilm.com)
- FUJIFILM Diosynth Biotechnologies assists in the manufacturing of recombinant protein vaccines used for multiple indications including Covid-19. (fujifilm.com)
Catalog3
- Here is the catalog of all recombinant proteins. (cusabio.com)
- The ED 50 for this effect is 1-4 μg/mL in the presence of 0.5 ng/mL of Recombinant Mouse IL-22 (Catalog # 582-ML ). (rndsystems.com)
- In addition to our diverse catalog and semi-custom recombinant protein products, we also offer comprehensive custom recombinant protein expression and production services to meet a variety of research needs. (avivasysbio.com)
Vivo4
- Photoreceptor-specific reporter gene expression is driven in vivo by a 350-bp segment of the beta-PDE 5' flanking sequence. (nih.gov)
- Mellerio also described a recently completed phase 3 trial with introduction of ex vivo gene-corrected keratinocytes, which has been associated with long-term improvements among patients with recessive dystrophic EB (RDEB). (medscape.com)
- protein interaction and localization studies in vitro and in vivo. (lu.se)
- 2003). The therapeutic effects of different genes delivered by ment of the hGFAP promoter used here has been extensively studied and lentiviral vectors have been documented in a number of animal found to be up-regulated after several different stimuli both in vitro and models, both in rodents and primates, and examples include delivery in vivo in transgenic mice (Brenner et al. (lu.se)
Yeast2
- The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. (antikoerper-online.de)
- A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. (antikoerper-online.de)
Eukaryotic2
- Over more than 16 years, CUSABIO has established five systems from prokaryotic to eukaryotic to express the recombinant protein. (cusabio.com)
- In addition, after transcription, a wide array of RNA-binding proteins interacts with cis -acting elements located mainly in the 3' untranslated region, determining the fate of mRNAs in eukaryotic cells. (frontiersin.org)
Localization3
- Furthermore, the possible implications to subcellular transporter localization and restoration of MTX sensitivity in drug-resistant tumors by RFC-based gene therapy are discussed. (aspetjournals.org)
- Within the retina, the reporter gene was specifically expressed in photoreceptors, consistent with the localization of endogenous beta-PDE. (nih.gov)
- The availability of multiple PAS in the same 3' regulatory region enables the inclusion or exclusion of 3' untranslated region (3'UTR) sequences, resulting in transcripts that may differ in particularities involving post-transcriptional processes such as stability, transport and translation ( Mayr, 2016 ), and even protein localization ( Berkovits and Mayr, 2015 ). (frontiersin.org)
Vitro2
- Moreover, PRIN2 and CSP41b form a distinct protein complex in vitro that binds DNA. (frontiersin.org)
- This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. (novusbio.com)
Transcript5
- The prin2.2 and csp41b-2 single mutants displayed pale phenotypes, abnormal chloroplasts with reduced transcript levels of photosynthesis genes and defects in embryo development. (frontiersin.org)
- Several transcript variants encoding different isoforms have been found for this gene. (origene.com)
- Polyadenylation is essential for the stability of the transcript, preventing the mRNA from being the target of posttranscriptional gene silencing (PTGS) via RNA-dependent RNA polymerase 6 (RDR6) in plants ( Luo and Chen, 2007 ). (frontiersin.org)
- Through APA, a single gene containing multiple PAS can generate a considerable number of transcript isoforms, thereby producing a highly diversified transcriptome ( Tian and Manley, 2013 ). (frontiersin.org)
- Two alternatively spliced transcript variants encoding the same protein have been described for MAP4K5. (thermofisher.com)
Homolog1
- Spectroscopic results showed 56% higher carbonic anhydrase activity in the presence of the recombinant DnaJ homolog compared to its absence. (bvsalud.org)
Vectors8
- Xiao, X., Li, J. & Samulski, R.J. Production of high-titer recombinant adeno-associated virus vectors in the absence of helper adenovirus. (nature.com)
- Collaco, R.F., Cao, X. & Trempe, J.P. A helper virus-free packaging system for recombinant adeno-associated virus vectors. (nature.com)
- High-titer recombinant adeno-associated virus production from replicating amplicons and herpes vectors deleted for glycoprotein H. (nature.com)
- Zhang, X. & Li, C.Y. Generation of recombinant adeno-associated virus vectors by a complete adenovirus-mediated approach. (nature.com)
- FUJIFILM Diosynth Biotechnologies' labs support the creation of gene therapy drugs and the vectors that deliver them. (fujifilm.com)
- FUJIFILM Diosynth Biotechnologies works with a variety of viral vectors and cell culture platforms to help develop the manufacturing process, leading to commercial production for gene therapy products. (fujifilm.com)
- This ®nding will lead to the development of lentiviral vectors with autoregulatory capacities that may be very useful for gene therapy to the brain. (lu.se)
- These vectors have a number of appealing features including the expression by using the machinery of the host cell instead of depending abilities to ef®ciently transduce cells in the central nervous system, on recombinant regulatory proteins. (lu.se)
Substances1
- This task can be accomplished by replacing tumor suppressor genes that have been lost or mutated, selectively inserting genes that produce cytotoxic substances, or modifying the immune system to destroy the tumor cells. (medscape.com)
Reporter2
- Toward this end, a hemagglutinin (HA) epitope tagged hRFC (hRFC-HA) was introduced into a bicistronic vector (pIRES2-EGFP), upstream of an enhanced green fluorescent protein (EGFP) reporter gene. (aspetjournals.org)
- Recombinant Lucia Luciferase Protein is a positive control for luciferase reporter assays. (invivogen.com)
Assays2
- Native P2 protein was present on T pallidum surfaces as shown by radioimmunoprecipitation assays with extrinsically labelled organisms. (bmj.com)
- This recombinant protein can be used for biological assays. (prosci-inc.com)
Homo1
Concentration5
- Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (rndsystems.com)
- Values on y-axis indicate binding response signals recorded for each protein in solution at the indicated concentration. (cellsignal.com)
- Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20? (joplink.net)
- The concentration of our recombinant proteins is measured using the absorbance at 280nm. (antibodies-online.com)
- The concentration of the protein is calculated using its specific absorption coefficient. (antibodies-online.com)
Binds1
- Thus this paper describes, for the first time, an MT gene identified from macroalgae which is induced by copper exposure and whose encoded protein product binds cadmium and copper. (portlandpress.com)
Phenotypes1
- This now enables investigation of how parameters such as clonal expansion relate to gene expression phenotypes [ 28 ], thereby providing novel insights not accessible by bulk sequencing (e.g. (nature.com)
Transcriptional1
Type4
- Lysosomal proteins: Lysosomal proteins are difficult to produce recombinantly due to the number and type of post-translational modifications that they have (e.g. glycosylation). (wikipedia.org)
- Interleukin 22 binding protein (IL-22BP), also known as CRF2-10, CRF2-X, and IL-22 RA2, is a 35-45 kDa secreted glycoprotein in the type II cytokine receptor family (CRF). (rndsystems.com)
- The region that contains Hdic will be compared between wild-type WGRC42 and its gene-edited, susceptible mutants. (usda.gov)
- This risk assessment was not dependent on the type of adju- decoding patterns of immunoregulatory serum proteins could re- vant therapy received by the patients. (lu.se)
Products2
- Aviva Systems Biology has been providing high-quality protein products to the scientific community for over 10 years. (avivasysbio.com)
- The course presents methods to express genes, which have products that are known, unknown, or not well characterised, and to analyse the gene products. (lu.se)
Synthesis2
- RNA codons and protein synthesis. (crossref.org)
- State-of-the-art algorithm used for plasmid design (Gene synthesis). (antibodies-online.com)
Spike protein1
Characterisation1
- Genetic Characterisation of Plasmodium falciparum Isolates with Deletion of the pfhrp2 and/or pfhrp3 Genes in Colombia: The Amazon Region, a Challenge for Malaria Diagnosis and Control. (cdc.gov)
Carbonic anhydrase1
- Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. (nih.gov)
Cysteine2
- Cystatins are proteins that inhibit cysteine proteases. (wikipedia.org)
- To investigate the possible existence of a metallothionein (MT) gene, the library was screened with degenerate probes designed using plant MT cysteine-rich motifs. (portlandpress.com)
Enhances1
Stability3
- The stability and function of the recombinant DnaJ protein under salt and pH stress conditions were evaluated. (bvsalud.org)
- The 3' regulatory regions have a great diversity of cis -regulatory elements directly involved in polyadenylation, stability, transport and mRNA translation, essential to achieve the desired levels of gene expression. (frontiersin.org)
- The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. (joplink.net)
Functional3
- Based on the results, DnaJ from the M. persicus can potentially be employed for improving the functional features of enzymes and other proteins in various applications. (bvsalud.org)
- Clinical evidence is limited, but Mellerio suggested that the theoretical potential for eliminating the source of abnormal transcription is the restoration of functional proteins essential for reversing skin fragility. (medscape.com)
- In order to investigate the functional role of miR-210 during TDI sensitization, BALBc mice were dermally exposed to TDI (0.5-4% v/v) and gene and protein expression were evaluated in the draining lymph nodes (dLN) and ears using RT-PCR and Western blot/ flow cytometry, respectively. (cdc.gov)
Production8
- Gene expression was analyzed by measuring beta-galactosidase activity in tissue homogenates or visualizing enzymatic activity or protein production at a cellular level by in situ histochemistry or immunocytochemistry. (nih.gov)
- Factors influencing recombinant adeno-associated virus production. (nature.com)
- Clark, K.R. Recent advances in recombinant adeno-associated virus vector production. (nature.com)
- Made in Germany - from design to production - by highly experienced protein experts. (antibodies-online.com)
- Mellerio described efforts to use an aminoglycoside to circumvent nonsense mutations that produce messenger RNA decay and impaired production of the proteins that prevent EB. (medscape.com)
- Production of perdeuterated proteins for neutron-scattering experiments. (lu.se)
- You will also learn strategies for improving the production of "difficult" proteins. (lu.se)
- The lectures will cover the current status of cell-based protein production systems and the theoretical aspects of the methodology. (lu.se)