Gene proteins expressed sequence tags. Medical search. Frequent questions

We identified zebrafish and Fugu TNFSF 13b homologues by genomic DNA database searches and determined that the predicted proteins share 68% and 76% sequence identity with trout TNFSF 13b. (usda.gov)
Three introns were identified in the rainbow trout, Atlantic salmon and zebrafish genomic sequences, and the intron locations correspond closely to those in the mammalian TNFSF 13b genes. (usda.gov)
Korf I, Flicek P, Duan D, Brent M: Integrating genomic homology into gene structure prediction. (crossref.org)
Stanke M, Tzvetkova A, Morgenstern B: AUGUSTUS at EGASP: using EST, protein and genomic alignments for improved gene prediction in the human genome. (crossref.org)
Burge C, Karlin S: Prediction of complete gene structures in human genomic DNA. (crossref.org)
The genomic organization of the human genes that encode protein prenyltransferases is shown in Figure 1 . (biomedcentral.com)
We have shown that approximately 14% of the newly sequencedexpressed sequence tags map completely or partially to genomic regions where there are no annotated genes orsplice variants and therefore, imply that these are novel genetic structures. (sfu.ca)
Genomic sequences of TEPs were retrieved after annotation of the B. glabrata genome. (ac.ir)
Therefore comparative genomic analysis of T . equi was undertaken to: 1) identify genes contributing to immune evasion and persistence in equid hosts, 2) identify genes involved in PBMC infection biology and 3) define the phylogenetic position of T . equi relative to sequenced apicomplexan parasites. (biomedcentral.com)
Comparative genomic analysis of T . equi revealed the phylogenetic positioning relative to seven apicomplexan parasites using deduced amino acid sequences from 150 genes placed it as a sister taxon to Theileria spp . (biomedcentral.com)
C: The genomic structure of the human preproapelin gene as found in the human PAC 454M7 clone (GenBank accession no. (phoenixpeptide.com)
The gene encoding the Ov-phy-1 open reading frame contained 11 introns, similar in structure to the gene encoding human prolyl 4-hydroxylase isoform I. Genomic Southern blot, EST and genomic PCR studies demonstrated that the O. volvulus genome contained between three and eight genes closely related to Ov-phy-1. (embl.de)
Phylogenetic analyses and genomic context imply a probable ancient horizontal gene transfer (HGT) from proteobacteria. (embl.de)
Advances in genomic sequencing technology are transforming medical discovery, enabling researchers to conduct comprehensive explorations across whole genomes for individuals and populations. (cdc.gov)
Array comparative genomic hybridization (aCGH) is a type of microarray now routinely used to identify deleted or duplicated regions of DNA sequence in specific chromosomes on a genome-wide basis. (msdmanuals.com)
He also noted concern regarding delays in referring specimens to the laboratory for testing, and emphasized the need for improvement in timeliness of reporting and tracking of the viruses referred for genomic sequencing. (who.int)
He thanked the regional and global specialized laboratories for their efforts to provide timely results of the genomic sequencing of wild polioviruses, and for their help in planning and monitoring polio eradication activities in the Region. (who.int)

High-density oligonucleotide arrays were used to identify genes differentially expressed between high and low ERG expressers. (nih.gov)
One-third of the 471 genes predicted to encode transcription factors are differentially expressed during sexual development of S. commune . (nature.com)
Quantitative PCR and high-throughput methods, such as expressed sequence tag analysis, have been used for measuring gene expression and identifying subsets of elm genes that are differentially expressed in the presence of O. novo-ulmi . (sisef.it)
These analyses have also helped identify genes that were differentially expressed in DED fungi grown under defined experimental conditions. (sisef.it)
Of the 233 randomly selected clones, 25 were identified as differentially expressed genes between 5MT resistant and 5MT sensitive wild type plants. (korea.ac.kr)

Sequencing of 16 M. persicae cDNA libraries generated 26,669 expressed sequence tags (ESTs). (nih.gov)
Automatic comparisons of data from expressed sequence tags (ESTs) with genes (for example using the program Acembly, for which the results are available from the NCBI AceView server [ 2 ]) shows that all the human protein prenyltransferase genes have multiple alternative splice variants. (biomedcentral.com)
Analysis of 20,257 high-quality ESTs yielded 6372 unique genes comprised of 3059 tentative consensus (TC) sequences and 3313 singletons (available at http://aksoylab.yale.edu ). (umd.edu)
It has been estimated that ~100,000 expressed sequence tags ( ESTs ) can be identified in humans. (wikidoc.org)
Expressed sequenced tags (ESTs) of 1 019 randomly selected clones were analyzed and then assembled 588 unigens. (korea.ac.kr)

Allen JE, Pertea M, Salzberg SL: JIGSAW, GeneZilla and GlimmerHMM: puzzling out the feature of human genes in the ENCODE regions. (crossref.org)
The IL-33 cDNA sequences encode 270 and 266 amino acid polypeptides for human and mouse, respectively, corresponding to full-length proteins with calculated masses of 30 and 29.9 kDa [ 2 ]. (hindawi.com)

The ongoing annotation of these genomes should spark a new wave of interest in the DED pathosystem, as it should lead to the formal identification of genes modulating parasitic fitness. (sisef.it)
McAuliffe J, Pachter L, Jordan M: Multiple-sequence functional annotation and the generalized hidden Markov phylogeny. (crossref.org)
Solovyev V, Kosarev P, Seledsov I, Vorobyev D: Automatic annotation of eukaryotic genes, pseudogenes and promoters. (crossref.org)
Curwen V, Eyras E, Andrews T, Clarke L, Mongin E, Searle SM, Clamp M: The Ensembl Automatic Gene Annotation System. (crossref.org)
In order to clarify the biological and pharmacological mechanisms of O. sinensis , we carried out the genome sequencing of H. sinensis, anamorph of O. sinensis , for the first time, described the annotation and gene expression, and analyzed the complete sequencing data (Li et al. (springeropen.com)
SUMMARY: SmashCommunity is a stand-alone metagenomic annotation and analysis pipeline suitable for data from Sanger and 454 sequencing technologies. (embl.de)
We also applied the protocol to UniRef database to improve the annotation of proteins with so far unknown function and identified some new instances of horizontal gene transfer. (embl.de)

These Unigenes represent a wide range of genes classified according to their characterization and function. (karger.com)
Genetic and molecular analyses have led to the identification, cloning and characterization of a few genes that contribute to parasitic fitness in the pathogens. (sisef.it)
There is paucity of information on functional relationship and characterization of Biomphalaria glabrata thioester-containing proteins ( Bg TEP) to other well-annotated homologues. (ac.ir)

Over the last two decades, several laboratories have used a wide range of techniques encompassing expressed sequence tag (EST) analysis, serial analysis of gene expression (SAGE), microarray and next-generation sequencing (NGS) technologies for analysing the chickpea transcriptomes. (intechopen.com)
Gene expression patterns in human liver cells exposed to tetrachloroethylene and it's metabolite using microarray analysis. (cdc.gov)
The Agilent eArray platform was used to design an M. persicae oligonucleotide microarray representing over 10,000 unique genes. (nih.gov)
These include previously unknown sequence data, a collection of expressed genes, molecular markers, and a DNA microarray that can be used to study aphid gene expression. (nih.gov)
This database will facilitate the design of microarray and other experiments to study screwworm gene expression on a larger scale than previously possible. (usda.gov)

The transporter was identified by probing a mammalian-expressed sequence tag database with 2 fungal mitochondrial ornithine carrier protein sequences. (medscape.com)
Actin is one of the most abundant proteins in yeast, mammalian and other eukaryotic cells. (elifesciences.org)
These proteins are needed for many other processes in yeast and other cells, including mammalian cells. (elifesciences.org)

The chromosome diagrams and the locations of the genes on the cytogenetic map are according to the NCBI MapViewer [ 67 ]. (biomedcentral.com)
In addition, TEP sequences deposited in NCBI protein database were also retrieved and utilized for sequence analysis. (ac.ir)
A total of 389 unigenes had significant homologies with known protein sequences at the NCBI database and the remaining 199 unigenes were designated unidentified genes. (korea.ac.kr)

Comparison of cDNA data from three aphid lineages identified single nucleotide polymorphisms that can be used as genetic markers and, in some cases, may represent functional differences in the protein products. (nih.gov)
Analysis of genetic diversity and population structure of peanut cultivars and breeding lines from China, India and the US using simple sequence repeat markers [J]. J Integr Plant Biol. (jipb.net)
addressed this question by studying yeast cells with genetic mutations affecting one or more of these proteins. (elifesciences.org)
Although amenable to genetic tagging, HSP101, PTEX150, EXP2 and PTEX88 could not be genetically deleted in P. berghei, in keeping with the obligatory role this complex is postulated to have in maintaining normal blood-stage growth. (pberghei.eu)
A large number of isoforms are caused by single nucleotide polymorphisms , small genetic differences between alleles of the same gene. (wikidoc.org)
A protein isoform, or "protein variant", [1] is a member of a set of highly similar proteins that originate from a single gene or gene family and are the result of genetic differences. (wikidoc.org)
Epigenetics refers to the study of changes in gene activity which do not involve alterations to the genetic code but still may passed down to at least one successive generation. (prohealth.com)
Next-generation sequencing technologies have dramatically changed the approach to genetic diagnosis. (msdmanuals.com)
This revolutionary and rapidly evolving technology has moved a significant portion of the technical aspects of genetic diagnosis to next-generation sequencing and has become the mainstay of genetic diagnosis. (msdmanuals.com)

hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes. (nih.gov)

Transcriptome analysis has facilitated the development of molecular markers such as simple sequence repeats (SSRs), single-nucleotide polymorphisms (SNPs) and potential intron polymorphisms (PIPs) that are being used to expedite the chickpea breeding programmes. (intechopen.com)
Xpro is a relational database that contains all the eukaryotic protein-encoding DNA sequences contained in GenBank with associated data required for the analysis of eukaryotic gene architecture. (edu.au)
Among the 17 tissues, the highest similarity in gene expression patterns was between human brain and testis, based on DDD and clustering analysis. (karger.com)
The differential gene expression data analysis was performed using GeneChip 4.0 software. (cdc.gov)
Until recently, however, functional analysis of the DED fungi was hampered by the lack of protocols for efficient gene knockout and by the unavailability of a full genome sequence. (sisef.it)
Batzoglou S, Pachter L, Mesirovi J, Berger B, Lander E: Human and mouse gene structure: comparative analysis and application to exon prediction. (crossref.org)
Durbin R, Eddy S, Krogh A, Mitchison G: Biological sequence analysis: Probabilistic models of proteins and nucleic acids. (crossref.org)
As we recently reported, analysis of 77 FHWT by whole genome sequencing (WGS, 58 patients) or whole exomic sequencing (WES,19 patients) identified 825 high-quality somatic, non-synonymous variants, with an average of 11 candidate mutations/case (range 2-42) (ref. 6 ). (nature.com)
Single-gene knockout-coupled omics analysis identifies C9orf85 and CXorf38 as two uncharacterized human proteins associated with ZIP8 malfunction. (nih.gov)
Phenolic Composition Analysis and Gene Expression in Developing Seeds of Yellow- and Black- seeded Brassica napus [J]. J Integr Plant Biol. (jipb.net)
Expression analysis of the three yolk proteins indicates that all are detected in females, while only the yolk protein with similarity to lipases, is expressed in males. (umd.edu)
Bg TEP relatedness to its other homologues as well as functional domain and protein-protein interaction analysis was performed. (ac.ir)
Our analysis identified 50 genes that will be useful for definitive phylogenetic classification of T . equi and closely related organisms. (biomedcentral.com)
To define the molecular basis of the IL-6-induced mitogenic response in myeloma cells, we applied STAR (subtractive transcriptional amplification of mRNA), a new differential expression analysis technology, to isolate mRNAs preferentially expressed in IL-6-treated versus untreated cultures of the factor-responsive myeloma cell line U266. (elsevierpure.com)
Northern blot analysis documented that two transcription factor genes chosen for further study, c-myc promoter-binding protein (MBP-1) and X-box binding protein 1 (XBP-1), were up-regulated in U266 cells about 3-fold relative to the cell cycle-dependent β-actin gene 12 h after IL-6 treatment. (elsevierpure.com)
Expressed sequence tag (EST) analysis and developmental polymerase chain reaction (PCR) studies demonstrated that Ov-phy-1 was expressed in L3 and adult parasites. (embl.de)
Systematic Analysis of ZnCys Transcription Factors Required for Development and Pathogenicity by High-Throughput Gene Knockout in the Rice Blast Fungus. (prelekara.sk)
2007) Genome-wide functional analysis of pathogenicity genes in the rice blast fungus. (prelekara.sk)
Here, through an in-depth phylogenetic clustering analysis of upwards of 200,000 whole-genome sequences, we reveal the presence of previously unreported and hitherto unidentified mutations and recombination breakpoints in Variants of Concern (VOC) and Variants of Interest (VOI) from Brazil, India (Beta, Eta and Kappa) and the USA (Beta, Eta and Lambda). (cdc.gov)
Genome sequencing on the Oxford Nanopore platform was performed using the ARTIC approaches for library preparation and bioinformatic analysis. (cdc.gov)

Genes contributing to the similarity include ribosomal protein (RP) genes as well as genes involved in transcription, translation and cell division. (karger.com)
The α subunits of protein prenyltransferases consist of tetratricopeptide repeats and are part of the tetratricopeptide repeat superfamily [ 5 ], which also includes functionally diverse proteins involved in transcription, co-chaperoning, protein transport, cell-cycle control and phosphorylation. (biomedcentral.com)
We developed a high-throughput gene knockout system, and using this system, we obtained null mutants of 104 fungal-specific Zn2Cys6 transcription factor (TF) genes by screening 8741 primary transformants in M. oryzae. (prelekara.sk)
Epi-", a Greek term meaning "above" or "on top of," in the term epigenetics , refers to modifications that do not change the DNA sequence itself but can turn genes on or off, affecting how transcription factors read genes. (prohealth.com)
Methylation generally serves to suppress gene transcription, meaning that genes with methyl tags are less likely to be expressed. (prohealth.com)
It does this by physically impeding the binding of transcription factors and other machinery necessary for gene expression. (prohealth.com)
Alternatively, it can also attract proteins that block gene transcription. (prohealth.com)
This results in a more open or "relaxed" chromatin structure, enabling greater access for the transcription machinery, thereby promoting gene expression. (prohealth.com)

Gene expression analyses using NGS platforms were carried out in the vegetative and reproductive tissues such as shoot, root, mature leaf, ﬂower bud, young pod, seed and nodule by various groups which resulted in identification of several tissue-specific transcripts. (intechopen.com)
Hence, it means that human testis could also play a crucial role in human speciation if these two tissues exhibit similar gene expression patterns. (karger.com)
Determining the large scale anatomy of gene expression patterns of various human tissues could draw a more convincing conclusion, and better our understanding of the correlation/inter-correlation among different tissues. (karger.com)
Bortoluzzi S, Alessi FD, Romualdi C, Danieli GA: Differential expression of genes coding for ribosomal proteins in different human tissues. (karger.com)
Trout TNFSF 13b RNA is expressed in immune tissues including the spleen, anterior kidney, and peripheral blood leukocytes but not in the liver. (usda.gov)
Identification of intrahepatic cholangiocarcinoma related genes by comparison with normal liver tissues using expressed sequence tags. (nih.gov)
The prepared antibody can be useful for the study of expression and distribution of IL-33 in various tissues at protein level and for the elucidation of its biofunctions and regulation mechanism in inflammatory diseases. (hindawi.com)

As a broad host range pathogen, M. phaseolina possesses a large number of pathogen-host interaction genes including those for adhesion, signal transduction, cell wall breakdown, purine biosynthesis, and potent mycotoxin patulin. (biomedcentral.com)

A: An alignment of amino acid sequences of rat preproapelin with human and bovine preproapelin. (phoenixpeptide.com)
B: An alignment of amino acid sequences of human apelin and angiotensin II. (phoenixpeptide.com)

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. (wikipedia.org)
Comparative analyses of its 38.5-megabase genome, which encodes 13,210 predicted genes, reveal the species's unique wood-degrading machinery. (nature.com)
To overcome the host plant defense response, M. phaseolina encodes a significant number of P450s, MFS type membrane transporters, glycosidases, transposases, and secondary metabolites in comparison to all sequenced ascomycete species. (biomedcentral.com)
Apelin gene encodes a pre-proprotein of 77 amino acids, with a signal peptide in the N-terminal region. (phoenixbiotech.net)

Alternative splicing of this gene generates at least 3 transcript variants diverging at the 5' terminus. (wikipedia.org)
Six variants (all identified by WGS) involved MLLT1 , a gene not previously reported to be involved in WT. (nature.com)
Each mutation was verified by Sanger sequencing of PCR products spanning the variants ( Fig. 1 ). (nature.com)
The TAG-VE will continue to critically appraise state-of-the-art methodologies for predicting further evolution of SARS-CoV-2 and will continue to rapidly determine the threat levels posed by new variants. (cdc.gov)
But the virus has changed significantly since Wuhan-Hu-1 was sequenced in January 2020, with variants of concern ranging from Alpha to Omicron. (cdc.gov)

However, there is no high-throughput gene knockout system constructed, and little is known about most of the genes in this fungus. (prelekara.sk)

Alexandersson M, Cawley S, Pachter L: SLAM: Cross-Species Gene Finding and Alignment with a Generalized Pair Hidden Markov Model. (crossref.org)
Carter D, Durbin R: Vertebrate gene finding from multiple-species alignments using a two-level strategy. (crossref.org)
2004. Virus-induced gene silencing in Solanum species . (berkeley.edu)

Flicek P, Keibler E, Hu P, Korf I, Brent MR: Leveraging the mouse genome for gene prediction in human: From whole-genome shotgun reads to a global synteny map. (crossref.org)

S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. (cancerindex.org)
The human IL-33 gene is located on chromosome 9p24.1, while its mouse counterpart can be found on the syntenic chromosome 19qC1 region [ 2 ]. (hindawi.com)

the α subunit of FT and GGT1 is encoded by the same gene, FNTA (see Table 1 ). (biomedcentral.com)
Gene structures and chromosomal locations of human protein prenyltransferase subunit genes. (biomedcentral.com)
This control is propagated through the alpha-subunit of prolyl 4-hydroxylase where protein levels were previously shown to rise fivefold with increasing cell density. (embl.de)
The presence of an intron, subunit fusion and expressed sequence tags covering parts of the gene exclude contamination and suggest a functional gene. (embl.de)
In the Fp subunit of SQR, and the closely related membrane-associated fumarate reductase of anaerobic organisms, FAD is covalently attached via an 8α-N(3)-histidyl linkage to a histidine (His) in a conserved sequence in the N-terminal part of the polypeptide. (lu.se)

In silico comparison of cDNA libraries identified aphid genes with tissue-specific expression patterns, and gene expression that is induced by feeding on Nicotiana benthamiana. (nih.gov)
We have initiated study of the genes of the New World Screwworm, Cochliomyia hominivorax, by synthesizing and sequencing DNA libraries of expressed genes from embryos, 2nd instar larvae, adult males and adult females. (usda.gov)
2003. Comparative analyses of potato expressed sequence tag libraries . (berkeley.edu)

Each sequence from this database was used to search public protein databases to assign putative function to as many database entries as possible. (usda.gov)
We analysed the putative fat body transcriptome based on homology to other gene products with known functions available in the public domain. (umd.edu)
T . equi has lost the putative genes for host cell transformation, or the genes were acquired by T . parva and T . annulata after divergence from T . equi . (biomedcentral.com)
Plasmodium parasites remodel their vertebrate host cells by translocating hundreds of proteins across an encasing membrane into the host cell cytosol via a putative export machinery termed PTEX (Plasmodium Translocon of EXported protein). (pberghei.eu)

10,200 protein-encoding genes were predicted based on the genome sequence. (springeropen.com)
The genes and proteins were predicted and annotated based on the genome sequence. (springeropen.com)
2005) The genome sequence of the rice blast fungus Magnaporthe grisea. (prelekara.sk)

The human coding sequence of MYH16 has a frameshift mutation that prevents translation of the functional product. (johnhawks.net)
The phylogenetics, functional domain and protein-protein interaction analyses suggest an immunological role for Bg TEP in B. glabrata . (ac.ir)
Overview of Genetics A gene, the basic unit of heredity, is a segment of DNA containing all the information necessary to synthesize a polypeptide (protein) or a functional RNA molecule. (msdmanuals.com)

New insights into the genome have led to the emergence of proteomics , the study of the structure and function of an individual's entire set of expressed proteins. (cdc.gov)
Proteomics is highly linked to genomics, since the blueprint for each protein is inscribed on an organism's genes. (cdc.gov)
Some challenges for translating proteomics include technical issues in measuring proteomic markers, identifying clinically useful markers in patient samples, the need for large sample sizes to be able to distinguish protein variability among samples, and challenges in implementing strict protocols for sample handling. (cdc.gov)

The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. (wikipedia.org)
The genes encoding CaaX protein prenyltransferases are considerably longer than those encoding non-CaaX subunits, as a result of longer introns. (biomedcentral.com)
It is interesting to note that the genes encoding both subunits of non-CaaX prenyltransferases are much smaller (about 6-9 kilobases, kb) than those of the CaaX prenyltransferases (about 30-76 kb). (biomedcentral.com)
In a global computational screening of conventional and metagenomic sequence data for NHases, we detected the two usually separated NHase subunits fused in one protein of the choanoflagellate Monosiga brevicollis, a recently sequenced unicellular model organism from the closest sister group of Metazoa. (embl.de)
The Fp and Ip subunits are both very conserved with respect to composition and polypeptide sequence [1]. (lu.se)
If the membrane-anchor is missing or defective, Fp and Ip subunits accumulate as soluble proteins in the cytoplasm. (lu.se)

In addition to the information found in the GenBank records, which includes properties such as sequence, position, length and description about introns, exons and protein-coding regions, Xpro provides annotations on the splice sites and intron phases. (edu.au)
Furthermore, Xpro validates intron positions using alignment information between the record's sequence and EST sequences found in dbEST. (edu.au)
The intron-containing genes in the Xpro are also classified as experimental or predicted based on the intron position validation and specific keywords in the GenBank records that are present in predicted genes. (edu.au)
The database currently contains information for 493 983 genes - 351 918 introncontaining genes and 142 065 intron-less genes. (edu.au)
Stanke M, Waack S: Gene prediction with a hidden Markov model and a new intron submodel. (crossref.org)
the differences in gene size therefore result from differing intron lengths. (biomedcentral.com)
Expressedsequence tags, which map across intron-exon boundaries (around 300), indicate possible alternative splice sites,while expressed sequence tags, which map near the ends of known transcripts (around 600), suggest extensionof the coding or untranslated regions. (sfu.ca)

Nucleotide positions of PAC 454M7 defining preproapelin gene exons (boxes) are shown at the top. (phoenixpeptide.com)
any "of several different forms of the same protein, arising from either single nucleotide polymorphisms, [4] differential splicing of mRNA, or post-translational modifications (e.g. sulfation, glycosylation, etc.)" [5] is called an isoform , or protein isoform . (wikidoc.org)

Desiccation tolerance of peanut seeds was achieved during 45 to 65 DAP (days after pegging) embryogenesis, while a set of low molecular weight (9 to 15.5 kD) heat-stable polypeptides was preferentially expressed. (jipb.net)
It was concluded that the heat-stable proteins may contribute to desiccation tolerance of the peanut seeds, and the low molecular weight heat-stable polypeptides may confer nonspecifieally heat tolerance on peanut storage proteins which were normally heat labile. (jipb.net)
In this study, we compare two environmental sequencing projects to find molecular evidence of transfer of microbes over vast geographical distances. (embl.de)
By cataloguing the molecular processes by which cells maintain and modify protein levels, proteomic studies offer another dimension of information that may help advance our understanding of health and disease. (cdc.gov)

Here we identify recurrent mutations within Wilms tumours that involve the highly conserved YEATS domain of MLLT1 (ENL), a gene known to be involved in transcriptional elongation during early development. (nature.com)
The genes encoding these proteins are highly conserved within the Trichinellids, are constituents of mL1 ESP and localized in the parasite stichosome. (westminster.ac.uk)

It is the only mushroom-forming fungus for which genes have been inactivated by homologous recombination. (nature.com)
CNF1 is the first reported TF gene that strongly and negatively regulates asexual development in the rice blast fungus, and CCA1, CNF1, CNF2, CONx1, GPF1, GTA1, MoCOD1 and PCF1 are required for pathogenicity. (prelekara.sk)

a "different sequence of a gene (locus)" [6] is called a variant . (wikidoc.org)

This process led to the identification of several gene coding regions with possible roles in sex determination in the developing screwworm embryo. (usda.gov)
Additionally, the identification of sex determination genes in the screwworm will assist in the development of a control technology based on transgenic strains of screwworm which produce only males, a technology which would be applicable to the USDA-APHIS screwworm eradication program's production facilities. (usda.gov)
2016. Identification of I-7 expands the repertoire of genes for resistance to Fusarium wilt in tomato to three resistance gene classes . (berkeley.edu)

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. (cancerindex.org)
In yeast, a member of the WASP family of proteins promotes the assembly of actin filaments around the site where endocytosis will occur. (elifesciences.org)

Parra G, Agarwal P, Abril J, Wiehe T, Fickett J, Guigo R: Comparative gene prediction in human and mouse. (crossref.org)
Gross S, Brent M: Using multiple alignments to improve gene prediction. (crossref.org)
Gross S, Do C, Batzoglou S: CONTRAST: de novo gene prediction using a semi-Markov conditional random field. (crossref.org)
Meyer I, Durbin R: Gene structure conservation aids similarity based gene prediction. (crossref.org)
Stanke M, Schoffmann O, Morgenstern B, Waack S: Gene prediction in eukaryotes with a generalized hidden Markov model that uses hints from external sources. (crossref.org)
Allen JE, Salzberg SL: JIGSAW: integration of multiple sources of evidence for gene prediction. (crossref.org)
Liu Q, Mackey A, Roos D, Pereira F: Evigan: a hidden variable model for integrating gene evidence for eukaryotic gene prediction. (crossref.org)
2014). Where annotations were not present or were different, the online Softberry server (http://linux1.softberry.com/berry.phtml) was used to predict mRNA and protein sequences using the FGNESH+ prediction algorithm (Solovyev et al. (molquest.com)
It supports state-of-the-art software for essential metagenomic tasks such as assembly and gene prediction. (embl.de)

Approximately 5,400 clones from each library were sequenced from both the 5' and 3' directions. (usda.gov)
From the resulting collection of STAR clones, sequence information was obtained for a total of 72 distinct transcripts. (elsevierpure.com)

Previous data has reported similarity between human brain and testis gene expression patterns. (karger.com)
Present results provide evidence to support the proposal that human testis and brain share the highest similarity of gene expression patterns. (karger.com)
In our laboratory, studies have been conducted to determine changes in gene expression patterns in cultured human cells exposed to TCE and TCA. (cdc.gov)
Whether difference in the expression patterns of these genes is associated with difference in carcinogenic potential of TCE and/or TCA needs to be elucidated. (cdc.gov)
By studying gene expression patterns of an organism, one can learn how that organism responds to its environment and thereby detect vulnerabilities in its lifecycle which can be exploited to develop control technologies. (usda.gov)
For further study of the differential expression of the genes, expression patterns of 12 genes related to various biological functions were evaluated in response to 5MT treatment in both the resistant plants and sensitive plants. (korea.ac.kr)
These expression patterns of the 12 genes suggested that the genes related to 5MT resistance in the rice mutants have a variety of functions, and yield remarkably diverse expression patterns upon 5MT treatment. (korea.ac.kr)

Tg2576 mice overexpress a mutant form of human amyloid precursor protein with the Swedish mutation (APP Sw ), resulting in high β-amyloid (Aβ) levels in the brain. (jneurosci.org)
Briefly, they contain the human APP 695 with the double mutation K670N and M671L (Swedish mutation) and are driven by the prion protein promoter. (jneurosci.org)
Our findings on the age of the inactivating mutation in the MYH16 gene raise the intriguing possibility that the decrement in masticatory muscle size removed an evolutionary constraint on encephalization, as suggested by the anatomy of the muscle attachments relative to the sutures. (johnhawks.net)

Extensive analyses of transcriptome have been carried out in chickpea, which is the third most important legume valued as a source of dietary protein and micronutrients. (intechopen.com)

We further found via RNA-seq that GPF1 and CNF2 have similar mechanisms in gene expression regulation related to pathogenicity. (prelekara.sk)

This lack of increase contrasts with previous reports as does the mRNA length: this is 50% larger as confirmed by sequencing the 3' end. (embl.de)

Biomphalaria glabrata transcriptome: identifi-cation of cell-signalling, transcriptional control and immune-related genes from open reading frame expressed sequence tags (ORESTES). (ac.ir)

To gain deeper insight into the biological and pharmacological mechanisms, we sequenced the genome of H. sinensis . (springeropen.com)
Exhibiting high consistence in sequence and structure, S100 family members are interchangeable in function and they show a wide spectrum of biological processes, including proliferation, apoptosis, migration, inflammation and differentiation and the like. (cancerindex.org)
The life cycle of a protein starts with a blueprint transcribed from a gene, followed by folding, which translates the blueprint to a three-dimensional structure that allows it to carry out its biological function, and ends with degradation. (cdc.gov)

The chromosomal locations and number of exons from protein prenyltransferase genes in the major eukaryotic model organisms are shown in Table 2 . (biomedcentral.com)

The complete genomes of these 8 FAdV-2 strains were sequenced. (bvsalud.org)

Our results demonstrate that SH3 (Src homology 3) domain-PRM (proline-rich motif) interactions involving multivalent linker proteins play central roles in concentrating NPFs at endocytic sites. (elifesciences.org)

Of these, 29 were found to correspond to known genes, 22 matched expressed sequence tags in public databases and 21 showed no sequence similarity to any existing entries. (elsevierpure.com)

The B. glabrata genome contains 14190 unannotated proteins after filtration with about 85% of its proteome annotated. (ac.ir)
the ability to create catalytically different proteins from the same gene increases the diversity of the proteome. (wikidoc.org)
Studies of the human proteome have enabled scientists to track protein synthesis, modification, and degradation over time and across different cell types. (cdc.gov)
Because the proteome is a continuously changing set of proteins that differs from cell to cell, it is challenging for scientists to capture and study it. (cdc.gov)
In 2014, scientists developed a draft map of the human proteome , which catalogued proteins encoded by over 17,000 human genes, or about 84% of all protein-coding genes in the human genome. (cdc.gov)

The expression of transthyretin, a protein shown to sequester Aβ and prevent amyloid fibril formation in vitro , and several genes in the insulin-signaling pathway, e.g., insulin-like growth factor-2, were increased selectively in the hippocampus of APP Sw mice. (jneurosci.org)
The precursor protein (pre-IL-33) was cleaved by caspase-1 into a mature 18-kDa protein (IL-33) in in vitro experiments using a recombinant protein [ 2 ]. (hindawi.com)
To expose any impact on export by ER retention of HSP101 masked during in vitro culture of P. falciparum blood stages, we tagged HSP101 and PTEX150 with kER in a transgenic P. berghei rodent malaria expressing DiCre and evaluated the impact of ER retrieval on parasite survival during mouse infection. (pberghei.eu)

The mutant MLLT1 protein shows altered binding to acetylated histone tails. (nature.com)
Moreover, MLLT1 -mutant tumours show an increase in MYC gene expression and HOX dysregulation. (nature.com)
The mutant RMgm-4541 contains a diCre expression cassette containing the two genes Cre59-FKBP and Cre60-FRB placed on either side of the bidirectional eef1a promoter. (pberghei.eu)
The mutant expresses PTEX150 tagged C-terminal with a KDEL ER-retrieval sequence. (pberghei.eu)
All of the tested 12 genes exhibited higher expression levels in mutant plants than wild type plants under the 5MT inhibition. (korea.ac.kr)

The resulting data provide new insights into how Zn2Cys6 TF genes regulate important traits during the infection cycle of this rice blast pathogen. (prelekara.sk)
It has paved the way for studying how genes regulate the most basic functions of the cell, including what proteins to produce and how to make them. (cdc.gov)

Yeh R, Lim L, Burge C: Computational inference of homologous gene structures in the human genome. (crossref.org)

The importance of S. commune as a model system is also exemplified by the fact that its recombinant DNA constructs will express in other mushroom-forming fungi 5 . (nature.com)
Although a recent study ( 22 ) reported enhanced avidity of H5N6 viruses for human-type receptors, recombinant clade 2.3.4.4 highly pathogenic influenza A virus H5 proteins from virus isolates in North America show a strict avian receptor-binding preference ( 23 ). (cdc.gov)
The recombinant protein, IL-33 containing a hexahistidine tag in the C-terminal, was expressed in Escherichia coli . (hindawi.com)
The rabbits were immunized with the purified recombinant protein. (hindawi.com)
The high titer (1:32000) polyclonal antibodies with high specificity were obtained by immunizing rabbits with the purified recombinant protein. (hindawi.com)
In this study, the cloning and expression of mouse IL-33 gene in Escherichia coli (E. coli ), purification of recombinant protein, and generation of polyclonal antibody against IL-33 are described. (hindawi.com)

F-box only protein 9 is a protein that in humans is encoded by the FBXO9 gene. (wikipedia.org)
Previous work showed that this gene had lost its normal function in humans, making it a pseudogene . (johnhawks.net)
By comparing the sequences of humans and other primates, Stedman's team estimated that MYH16 had lost its function in human ancestors around 2.4 million years ago. (johnhawks.net)
They looked at more than 1100 base pairs of sequence from a chimpanzee, orangutan, macaque, and a dog, and they found that the coding sequence in humans has a small number of unexpected amino-acid-coding changes-changes that wouldn't be likely if the gene product was still being maintained by selection. (johnhawks.net)
There are three different protein prenyltransferases in humans: farnesyltransferase (FT) and geranylgeranyltransferase 1 (GGT1) share the same motif (the CaaX box) around the cysteine in their substrates, and are thus called CaaX prenyltransferases, whereas geranylgeranyltransferase 2 (GGT2, also called Rab geranylgeranyltransferase) recognizes a different motif and is thus called a non-CaaX prenyltransferase [ 1 ]. (biomedcentral.com)

The chemical tags are referred to as "epigenetic" markers because they exist outside of ( epi- ) the actual sequence of DNA ( -genetics ). (answersingenesis.org)
Epigenetics, the study of changes in gene expression not caused by alterations in the DNA sequence, has revolutionized our understanding of genetics and its impact on health and longevity. (prohealth.com)

S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. (cancerindex.org)

In particular, we describe the immune-related products, reproductive function related yolk proteins and milk-gland protein, iron metabolism regulating ferritins and transferrin, and tsetse's major energy source proline biosynthesis. (umd.edu)

After overnight ex vivo culture to allow for tag switching, equal numbers of DMSO or rapamycin-treated parasites were IV injected into naïve mice. (pberghei.eu)

Search the gene expression profiles from curated DataSets in the Gene Expression Omnibus (GEO) repository. (cancerindex.org)

Two compounds, Compound 1 (1,4-dihydrophenanthrolin-4-one-3-carboxylic acid) and Compound 5 [8-(N-butyl-N-ethylcarbamoyl)-1,4-dihydrophenathrolin-4-one-3-carboxylic acid], with comparable potencies in vivo, were chosen to investigate the effect of the inhibition of the hydroxylation of newly synthesized uterine collagen on the turnover of this protein in vivo. (embl.de)

Anatomy12

Organisms16

Diseases1

Chemicals and Drugs20

Analytical, Diagnostic and Therapeutic Techniques and Equipment22

Phenomena and Processes51

Disciplines and Occupations5

Technology, Industry, Agriculture4

Information Science24

Health Care2

Genomic17

Differentially expressed5

ESTs5

Encode2

Annotation7

Characterization3

Microarray5

Mammalian3

NCBI3

Genetic9

Representing over 10,0001

Analysis20

Transcription8

Tissues7

Signal transduction1

Amino acid sequences2

Encodes4

Variants5

High-throughput1

Species3

Whole genome1

Chromosome2

Subunit5

Libraries3

Putative4

Genome sequence3

Functional3

Proteomics3

Subunits6

Intron7

Nucleotide2

Molecular4

Highly conserved2

Fungus2

Locus1

Identification3

Family of proteins2

Prediction9

Clones2

Patterns7

Mutation3

Analyses1

Expression Regulation1

MRNA1

Transcriptional1

Biological3

Exons1

Genomes1

Homology1

Similarity1

Proteome5

Vitro3

Mutant5

Regulate2

Human genome1

Recombinant6

Humans5

Genetics2

Regulation1

Biosynthesis1

Parasites1

Datasets1

Vivo1