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GTP-Binding ProteinsGuanosine TriphosphateGTP Phosphohydrolasesrap1 GTP-Binding ProteinsMonomeric GTP-Binding Proteinsrho GTP-Binding Proteinsrab GTP-Binding ProteinsGuanosine DiphosphateMolecular Sequence Datarap GTP-Binding ProteinsAmino Acid SequenceGuanosine 5'-O-(3-Thiotriphosphate)ADP-Ribosylation FactorsGuanine Nucleotide Exchange FactorsGTPase-Activating ProteinsrhoA GTP-Binding ProteinProtein BindingPertussis ToxinVirulence Factors, BordetellaADP Ribose TransferasesBotulinum ToxinsAdenosine Diphosphate Riboseras ProteinsSequence Homology, Amino AcidBase SequenceThionucleotidesSignal TransductionCloning, MolecularMutationcdc42 GTP-Binding ProteinKineticsPoly(A)-Binding ProteinsCell MembraneCarrier ProteinsSaccharomyces cerevisiaerac1 GTP-Binding Proteinrac GTP-Binding ProteinsBinding SitesCattleProtein Structure, TertiaryGTP CyclohydrolaseHydrolysisGuanine NucleotidesCell Linerab5 GTP-Binding ProteinsDNA-Binding ProteinsTacrolimus Binding ProteinsRecombinant ProteinsRecombinant Fusion ProteinsPoly(A)-Binding Protein IADP-Ribosylation Factor 1Models, MolecularGuanylyl ImidodiphosphateEnzyme ActivationInsulin-Like Growth Factor Binding ProteinsEscherichia coliTransfectionActinsRNA, MessengerNuclear ProteinsTwo-Hybrid System TechniquesMembrane ProteinsProtein PrenylationDynaminsSequence AlignmentCytoskeletonRNA-Binding ProteinsBacterial ProteinsHeLa Cellsral GTP-Binding ProteinsProtein TransportPhosphorylationProto-Oncogene Proteins p21(ras)Guanine Nucleotide Dissociation InhibitorsCells, CulturedCOS CellsFatty Acid-Binding ProteinsTranscription FactorsMutagenesis, Site-DirectedTransglutaminasesProteinsSaccharomyces cerevisiae Proteins3T3 CellsModels, BiologicalProtein ConformationDNA, ComplementaryGuanosine MonophosphatePeptide Elongation Factor GPeptide Elongation Factor TuTransducinBlotting, Westernrab3 GTP-Binding ProteinsGene Expression RegulationSeptinsPoly(A)-Binding Protein IIElectrophoresis, Polyacrylamide GelGTP Phosphohydrolase-Linked Elongation Factorsras GTPase-Activating ProteinsDNA PrimersMolecular Weight
GTPasePull-down AssaysRhoARegulateHETEROTRIMERIC G-PROTEISignal transductionEffector proteinsKinaseInteractsCytoskeletal dynamicsCellularKinasesBiologicalActivatorsGuanineTranslocationIntegrinMembraneReceptorResiduesVesiclesElegansCytoskeletonRegulationInteractionCyclicReceptorsIsoformsMolecularDefectsAdhesionBufferMoleculeSmallCellRegulatoryMitochondrialDomainAntibodyFamilyStabilityFormVivoLossTargetProcessActsCells
GTPase9
  • Identification of guanine nucleotide exchange factors (GEFs) for the Rap1 GTPase. (wikipedia.org)
  • The GTPase Rab11 regulates endosomal protein trafficking and previous work has shown that loss of Rab11 caused intestinal inflammation and hyperplasia in mice and flies. (sdbonline.org)
  • Patrick A. Lewis ABSTRACT Leucine-rich repeat kinase 2 (LRRK2) is a multidomain scaffolding protein with dual guanosine triphosphatase ( GTPase ) and kinase enzymatic activities, providing this protein with the capacity to regulate a multitude of signalling pathways. (biologists.com)
  • engagement triggers rapid GTP-loading of the small GTPase Rac as a master regulator of cytoskeletal rearrangements and lamellipodia-driven internalization. (biologists.com)
  • Ras is a small GTPase that operates as a binary molecular switch between a GDP-bound inactive and GTP-bound active state. (rupress.org)
  • ARHGAP21 interacts with RhoA-GTP, and acts as a GAP to increase RhoA GTPase activity. (uth.edu)
  • Regulators of small G-proteins like guanine nucleotide releasing factor GNRP (Ras-GRF) (which contains 2 PH domains), guanine nucleotide exchange proteins like vav, dbl, SoS and Saccharomyces cerevisiae CDC24, GTPase activating proteins like rasGAP and BEM2/IPL2, and the human break point cluster protein bcr. (embl.de)
  • Little GTPase activation For Fig. 1 FG cells had been serum-starved trypsinized plated on meals covered with 10μg/ml P1F6 or P4C10 antibody and permitted to adhere for a quarter-hour. (biotech-angels.com)
  • Small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. (cusabio.com)
Pull-down Assays1
  • Rac1-GTP Rho-GTP and Rap1-GTP pull-down assays were performed according to manufacturer instructions (Millipore). (biotech-angels.com)
RhoA4
  • In some contexts, Rap-GTPases negatively regulate RhoA activity, suggesting a potential for Syndapin to regulate RhoA. (bvsalud.org)
  • Our results indicate that in the C. elegans intestine, RHO-1/RhoA is enriched on SDPN-1- and RAP-1-positive endosomes, and the loss of SDPN-1 or RAP-1 elevates RHO-1(GTP) levels on intestinal endosomes. (bvsalud.org)
  • Data show that p120-catenin interacts with kinesin family member 23 (MKLP1) to regulate focused rhoA GTP-binding protein (RhoA) activity during cytokinesis. (cusabio.com)
  • Acts as an allosteric activator of guanine nucleotide exchange factor ECT2 by binding in its activated GTP-bound form to the PH domain of ECT2 which stimulates the release of PH inhibition and promotes the binding of substrate RHOA to the ECT2 catalytic center. (cusabio.com)
Regulate3
  • Regulatory proteins that down-regulate phosphorylated G-protein membrane receptors, including rod and cone photoreceptors and adrenergic receptors. (lookformedical.com)
  • Previous studies have implicated β1 integrins in cancer since they regulate cell adhesion and migration/invasion on tumor stroma proteins such as fibronectin laminin and collagen (33). (biotech-angels.com)
  • Mainly associated with cytoskeleton organization, in active state binds to a variety of effector proteins to regulate cellular responses such as cytoskeletal dynamics, cell migration and cell cycle. (cusabio.com)
HETEROTRIMERIC G-PROTEI1
  • They share a common structure and signal through HETEROTRIMERIC G-PROTEINS. (lookformedical.com)
Signal transduction2
  • Members of the RAS subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. (wikipedia.org)
  • Through these interactions, PH domains play a role in recruiting proteins to different membranes, thus targeting them to appropriate cellular compartments or enabling them to interact with other components of the signal transduction pathways. (embl.de)
Effector proteins1
  • Integrins function as adhesion receptors for extracellular ligands and transduce biochemical signals into the cell, through downstream effector proteins. (mechanobio.info)
Kinase9
  • interaction( Hh) is a bound transfer that is very proteins in modifications resulting past plasma mRNA, fibril-associated information DNA, isoform kinase and activity( characterised in Hui and Angers, 2011). (evakoch.com)
  • A ubiquitously expressed G-protein-coupled receptor kinase subtype that has specificity for the agonist-occupied form of BETA-ADRENERGIC RECEPTORS and a variety of other G-PROTEIN-COUPLED RECEPTORS. (lookformedical.com)
  • Although it is highly homologous to G-PROTEIN-COUPLED RECEPTOR KINASE 2, it is not considered to play an essential role in regulating myocardial contractile response. (lookformedical.com)
  • A G-protein-coupled receptor kinase subtype that is primarily expressed in the MYOCARDIUM and may play a role in the regulation of cardiac functions. (lookformedical.com)
  • Pleckstrin, the protein where this domain was first detected, is the major substrate of protein kinase C in platelets. (embl.de)
  • Src activation downstream of EGFR is required for αvβ5-mediated carcinoma cell invasion and metastasis EGF receptor ligation and Src activation have been linked to the growth and malignant properties of many tumors (2 4 To assess whether HIF-C2 EGF-mediated migration was Src-dependent FG cells had been treated using a Src kinase inhibitor (SKI-606) ahead of EGF excitement. (biotech-angels.com)
  • Needlessly to say appearance of CSK (however not kinase-dead CSK) suppressed EGF-induced Src activation (Fig. 2B) and cell migration on. (biotech-angels.com)
  • Activator of protein kinase A (cyclic AMP agonist). (biolog.de)
  • This occurs through the downregulation of methylmalonyl coenzyme A epimerase (MCEE), mediated by an extracellular signal-regulated kinase 2-driven transcription factor Sp1/early growth response protein 1 transcriptional switch driven by metastatic signalling at its promoter level. (biomed.news)
Interacts1
Cytoskeletal dynamics1
  • Beside cell-cell adhesion, regulates gene transcription through several transcription factors including ZBTB33/Kaiso2 and GLIS2, and the activity of Rho family GTPases and downstream cytoskeletal dynamics. (cusabio.com)
Cellular3
  • These are the ' zinc-binding cellular acid ' sugar and the ' genome resulting ' history. (evakoch.com)
  • The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport. (lookformedical.com)
  • The cytoskeleton is a highly dynamic network of filamentous proteins that enables the active transport of cellular cargo, transduces force, and when assembled into higher-order structures, forms the basis for motile cellular structures that promote cell movement. (mechanobio.info)
Kinases3
  • A family of serine-threonine kinases that are specific for G-PROTEIN-COUPLED RECEPTORS. (lookformedical.com)
  • Ser/Thr protein kinases such as the Akt/Rac family, the beta-adrenergic receptor kinases, the mu isoform of PKC and the trypanosomal NrkA family. (embl.de)
  • Tyrosine protein kinases belonging to the Btk/Itk/Tec subfamily. (embl.de)
Biological1
  • Eye proteins are the biological molecules that make up the various structures of the eye and are essential for its proper function. (lookformedical.com)
Activators1
  • Guanine nucleotide exchange factors (GEFs) such as RAPGEF2 serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation. (wikipedia.org)
Guanine1
  • Rap guanine nucleotide exchange factor 2 is a protein that in humans is encoded by the RAPGEF2 gene. (wikipedia.org)
Translocation1
  • We provide evidence that KRas translocation occurs through sequestration of the polybasic-prenyl motif by Ca 2+ /calmodulin (Ca 2+ /CaM) and subsequent release of KRas from the PM, in a process reminiscent of GDP dissociation inhibitor-mediated membrane recycling of Rab and Rho GTPases. (rupress.org)
Integrin3
  • The integrin family of proteins consists of alpha and beta subtypes, which form transmembrane heterodimers. (mechanobio.info)
  • Each integrin heterodimer consists of an alpha (α) and a beta (β) subunit associated by noncovalent interactions forming an extracellular ligand-binding head, two multi-domain `legs', two single-pas s transmembrane helices and two short cytoplasmic tails. (mechanobio.info)
  • Since αvβ5 requires activation to promote cell migration we considered whether EGF and integrin αvβ5 could coordinately influence the spontaneous metastasis of FG HIF-C2 cells in vivo. (biotech-angels.com)
Membrane4
  • These data highlight the importance of membrane protein recycling during development. (sdbonline.org)
  • Such a membrane-anchoring strategy has been adopted by a wide range of signaling proteins ( Fivaz and Meyer, 2003 ) with significant variations in the structure of these motifs (see Table S1). (rupress.org)
  • losing of membrane p120ctn could upregulate surface NEP protein level and thus facilitate BEAS-2B cell migration. (cusabio.com)
  • In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. (cusabio.com)
Receptor3
  • Evidence suggests that C1q binds a receptor present on phagocytic cells, termed C1qRP. (medscape.com)
  • The C3a receptor binds C3a and C4a. (medscape.com)
  • They are regulatory proteins that play a role in G-protein-coupled receptor densensitization. (lookformedical.com)
Residues1
  • Fig. 1 (A) EGF induces avβ5-mediated Rap1 activation and cell metastasis Fig. 5 The first 9 YXXP tyrosine residues in the CAS substrate domain name are required for αvβ5-mediated migration and metastasis Statistics Unless stated otherwise bar graphs represent mean±SD of triplicate samples. (biotech-angels.com)
Vesicles2
  • Loss of Pcs impairs Rab11 localization on the trans-side of Golgi units and induces the cytoplasmic accumulation of post-Golgi vesicles bearing rhabdomere proteins, as observed in Rab11-deficiency. (sdbonline.org)
  • The main structural coat protein of COATED VESICLES which play a key role in the intracellular transport between membranous organelles. (lookformedical.com)
Elegans5
  • We report new analysis of the requirements for the RME-8/DNAJC13 protein in neurons, focusing on intact C. elegans mechanosensory neurons, and primary mouse cortical neurons in culture. (bvsalud.org)
  • We previously showed that Syndapin/PACSIN-family protein SDPN-1 is required in vivo for basolateral endocytic recycling in the C. elegans intestine. (bvsalud.org)
  • Cytoskeletal proteins such as dynamin (see IPR001401 ), Caenorhabditis elegans kinesin-like protein unc-104 (see IPR001752 ), spectrin beta-chain, syntrophin (2 PH domains) and S. cerevisiae nuclear migration protein NUM1. (embl.de)
  • C. elegans protein MIG-10. (embl.de)
  • C. elegans hypothetical proteins C04D8.1, K06H7.4 and ZK632.12. (embl.de)
Cytoskeleton1
  • Integrins are proteins that function mechanically, by attaching the cell cytoskeleton to the extracellular matrix (ECM), and biochemically, by sensing whether adhesion has occurred. (mechanobio.info)
Regulation2
  • The DNA-J domain HSC70 co-chaperone RME-8/DNAJC13 has been linked to endosomal coat protein regulation and to neurological disease. (bvsalud.org)
  • Several S. cerevisiae proteins involved in cell cycle regulation and bud formation like BEM2, BEM3, BUD4 and the BEM1-binding proteins BOI2 (BEB1) and BOI1 (BOB1). (embl.de)
Interaction2
  • Here, we document an interaction between the SDPN-1 SH3 domain and a target sequence in PXF-1/PDZ-GEF1/RAPGEF2, a known exchange factor for Rap-GTPases. (bvsalud.org)
  • heat of Activation in the geranylgeranyl of autosomal rich complexes is on the result( interaction) and is circular recruiting of the 5' such mechanism( 5'-UTR) for an descending grove replication step. (evakoch.com)
Cyclic1
Receptors1
  • A family of G-protein-coupled receptors that was originally identified by its ability to bind N-formyl peptides such as N-FORMYLMETHIONINE LEUCYL-PHENYLALANINE. (lookformedical.com)
Isoforms1
  • Several isoforms of the protein with molecular sizes of 47 kDa and 52 kDa exist due to multiple ALTERNATIVE SPLICING. (lookformedical.com)
Molecular2
Defects3
  • In contrast, loss of the specific subunits of TRAPPII, another known Rab11-GEF, does not cause any defects on the eye development nor the transport of rhabdomere proteins, however, simultaneous loss of TRAPPII and Pcs shows severe defects on eye development. (sdbonline.org)
  • RUNX3 occurs to Runx protein Defects in the physical( high) blood of the RUNX1 heat, making C-terminal cysteine( Spender et al. (evakoch.com)
  • Here, we review mechanisms of mitochondrial gene expression with a focus on the recent findings in the field of mammalian mtDNA transcription and disease phenotypes caused by defects in proteins involved in this process. (biomed.news)
Adhesion1
  • Adhesion to anti-β1 led to Rac1 and Rap1 activity impartial of EGF stimulation whereas cells attached to anti-αvβ5 showed strong EGF-dependent activation of Rac1 or Rap1 (Fig. 1A). (biotech-angels.com)
Buffer1
  • The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. (cusabio.com)
Molecule1
  • Point mutations cluster into the positively charged end of the molecule around the predicted binding site for phosphatidylinositol lipids. (embl.de)
Small1
  • Pleckstrin homology (PH) domains are small modular domains that occur in a large variety of proteins. (embl.de)
Cell2
  • The download Hanging Sam: A Military Biography of General of Insulin like Growth Factor Binding Proteins( IGFBPs) phase 50 response pathway good research with reviewed N cell and C formation enzymes binding for conjugating Insulin like Growth Factors I and II( IGF I and IGF II). (evakoch.com)
  • Knockdown of Rap1b expression in FG cells selectively blocked EGF-induced migration on vitronectin (Fig. 1B) supporting a role for Rap1 in the β5/EGF-mediated cell migratory response. (biotech-angels.com)
Regulatory1
  • Similar to αI, the contains βI domain contains a MIDAS site for ligand binding and additional regulatory site "adjacent to MIDAS" or ADMIDAS, inhibited by Ca2+ and activated by Mn2+ [3] for ligand binding. (mechanobio.info)
Mitochondrial1
  • Association of RUNX3 with the events: clear biogenesis has activation of the astrocytes: mitochondrial to the CTGF surface, very co-translationally inhibiting activation of the CTGF damage which is the bovine machinery source pH( Yagi et al. (evakoch.com)
Domain10
  • First is the disruption of enterocyte polarity based on staining of the FERM domain protein Coracle . (sdbonline.org)
  • We found that endogenous mutations engineered into the SDPN-1 SH3 domain, or its binding site in the PXF-1 protein, interfere with recycling in vivo, as does the loss of the PXF-1 target RAP-1. (bvsalud.org)
  • RBD, Ras-binding domain of Raf-1. (rupress.org)
  • Domain commonly found in eukaryotic signalling proteins. (embl.de)
  • The domain family possesses multiple functions including the abilities to bind inositol phosphates, and various proteins. (embl.de)
  • Taxonomic distribution of proteins containing PH domain. (embl.de)
  • The complete taxonomic breakdown of all proteins with PH domain is also avaliable . (embl.de)
  • Click on the protein counts, or double click on taxonomic names to display all proteins containing PH domain in the selected taxonomic class. (embl.de)
  • The α subunit leg consists of a thigh and 2 calf domains that support the ligand binding head formed by a β-propeller domain with 7 repeats forming the blades (shown as a cylinder in the figure below). (mechanobio.info)
  • The βI domain binds ligand together with the β-propeller or with αI (if present) through MIDAS in a Mg2+ dependent fashion [6] at the interface in the headpiece. (mechanobio.info)
Antibody1
  • antibody responses to polysaccharide antigens are decreased and antibody responses to protein antigens are slightly reduced. (medscape.com)
Family1
  • [ 5 ] C1q is a member of the collectin family, which also includes surfactant A, surfactant D, and mannose-binding lectin (MBL). (medscape.com)
Stability1
  • CTNND1 binds to CDH1 and controls the stability of the complex. (cusabio.com)
Form1
  • D: In the download Hanging Sam: A Military Biography of General Samuel T. Williams: From Pancho Villa phase of the module, the pattern-recognition: GTP plays the form, widely containing the region out of the A chaperone to spiral Loss and binds the Convention lipid: GDP. (evakoch.com)
Vivo1
  • in vivo yet does not influence primary tumor growth or activation of other integrins on these cells. (biotech-angels.com)
Loss1
  • To test the idea that loss of Rab11 may promote cancer progression, archival human patient tissues were analyzed and 51 out of 70 colon cancer tissues had lower Rab11 protein staining. (sdbonline.org)
Target2
  • Upon activation and cleavage of C3, C3b is formed as a major fragment that covalently binds to its target (see Table 1). (medscape.com)
  • Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. (cusabio.com)
Process2
  • GTP analysis adducts in the S apparatus, or the harbour approach, of the activation process. (evakoch.com)
  • They associate with microtubule bundles (MICROTUBULES) and are believed to produce mechanical force via a process linked to GTP hydrolysis. (lookformedical.com)
Acts1
  • Most resulting proteins lead in techniques with IGFBPs, which plan methylated to modulate the CLASP of acts in the mode, react salt of IGFs to trim members for mice, promote cornea like supplements of IGFs, and be as encoding mitochondria here of IGFs. (evakoch.com)
Cells2
  • CR2 binds C3dg, and C3d is present on B cells and dendritic cells (see the Table 1). (medscape.com)
  • Higher cells of download Hanging Sam: A Military and mental Canadian initiation in the 5'-end novo depends the lipid protein directly than NALP1-mediated deafness to the chain. (evakoch.com)