Hepatitis C virus has a positive sense single-stranded RNA genome. (wikipedia.org)
The HCV genome consists of a 9.6 kb uncapped linear single-stranded RNA with positive polarity. (xiahepublishing.com)
Consists2
The genome consists of a single open reading frame that is 9,600 nucleotide bases long. (wikipedia.org)
This is why on publicly available databases, such as the European Bioinformatics Institute, the viral proteome only consists of 2 proteins. (wikipedia.org)
Responses2
Our mRNA-based vaccine demonstrated potent humoral and cellular responses throughout the time points of the murine model, inducing viral neutralizing antibodies, antibody-dependent cell cytotoxicity effect mediating antibodies and cross-reactive CD8+ T cell immune responses. (bvsalud.org)
Imaging cytomegalovirus infection and ensuing immune responses. (resist-cluster.de)
Domain2
domain 3 (residues 175-191) is highly hydrophobic and acts as a signal sequence for E1 envelope protein. (wikipedia.org)
Crystal structure of the N-terminal domain of the effector protein SidI of Legionella pneumophila reveals a glucosyl transferase domain. (resist-cluster.de)
Envelope proteins4
Both envelope proteins (E1 and E2) are highly glycosylated and important in cell entry. (wikipedia.org)
The thus replicated HCV genomic RNA is packaged by the Core protein and envelope proteins (E1 protein and E2 protein), which are structural proteins, so that new viral particles are formed. (justia.com)
HCV envelope proteins are considered to play a key role in the binding of HCV to cell surfaces. (justia.com)
Thus, research has been conducted for preparation of antibodies against envelope proteins in blood serum samples of HCV patients. (justia.com)
Hepatitis4
The purpose of this Funding Opportunity Announcement (FOA) is to support novel strategies for the rational design of vaccines against hepatitis C virus (HCV), to assess the vaccines for their ability to induce protective immune responses, and to select candidates for preclinical development and clinical testing. (nih.gov)
For this purpose, the detection of antibodies to non-structural 3ABC protein is suitable for identification of virus activity in the animals exposed to FMDV infection. (bvsalud.org)
This protein can be used as a suitable diagnostic antigen to detect antibodies following FMDV infection. (bvsalud.org)
Proteases1
The large pre-protein is later cleaved by cellular and viral proteases into the 10 smaller proteins that allow viral replication within the host cell, or assemble into the mature viral particles. (wikipedia.org)
Viral particles1
Previously, a B19V genome of defined consensus sequence was designed, synthesized and cloned in a complete and functional form, able to replicate and produce infectious viral particles in a producer/amplifier cell system. (bvsalud.org)
Replication5
citation needed] At the 5′ and 3′ ends of the RNA are the untranslated regions (UTR), that are not translated into proteins but are important to translation and replication of the viral RNA. (wikipedia.org)
NS1 (p7) protein is dispensable for viral genome replication but plays a critical role in virus morphogenesis. (wikipedia.org)
The genome terminal regions were maintained in a form able to sustain viral replication, while the internal region was clipped to include only the left-side genetic set, containing the coding sequence for the functional NS1 protein. (bvsalud.org)
Following transfection in UT7/EpoS1 cells, this minigenome still proved competent for replication, transcription and production of NS1 protein. (bvsalud.org)
In addition, we found that a smaller proportion of cells supported miR-122-independent replication compared a population of cells supporting miR-122-dependent replication, while viral protein levels per positive cell were similar. (bvsalud.org)
Structural1
The detection of antibody to non-structural protein (NSP) of Foot-and-mouth disease virus (FMDV) is the reliable diagnostic method for differentiating infected from vaccinated animals (DIVA). (bvsalud.org)
Terminal1
citation needed] The proteins of this virus are arranged along the genome in the following order: N terminal-core-envelope (E1)-E2-p7-nonstructural protein 2 (NS2)-NS3-NS4A-NS4B-NS5A-NS5B-C terminal. (wikipedia.org)
Receptor3
It prevents CD81 from latching onto its respective receptor on the virus. (wikipedia.org)
E1 serves as the fusogenic subunit and E2 acts as the receptor binding protein. (wikipedia.org)
First, HCV binds to a specific protein (virus receptor) on the cell surface and is incorporated by endocytosis into the host cell. (justia.com)
The HCV genome encodes a precursor protein that is converted into 10 types of virus protein (i.e. (justia.com)
After each virus protein has been generated by processing, the HCV genomic RNA is replicated by RNA polymerase, which is one of the generated virus proteins. (justia.com)
Ability1
Further, the B19V minigenome was able to complement B19-derived, NS1-defective genomes, restoring their ability to express viral capsid proteins. (bvsalud.org)
Active2
This single open reading frame is translated to produce a single protein product, which is then further processed to produce smaller active proteins. (wikipedia.org)
In conclusion, we presented SUMO fusion for the enhanced expression in E. coli and purification of active recombinant 3ABC protein using non-denaturing conditions without refolding steps. (bvsalud.org)
Highly1
domain 3 (residues 175-191) is highly hydrophobic and acts as a signal sequence for E1 envelope protein. (wikipedia.org)
Activity1
The mature nonstructural proteins (NS2 to NS5B) generation relies on the activity of viral proteinases. (wikipedia.org)
In this study, 3ABC gene was fused with SUMO (small ubiquitin-like modifiers) fusion system which significantly enhanced expression of recombinant 3ABC protein in E. coli. (bvsalud.org)