• Learn about the Conferma ® ELISA development and manufacturing methods that provide strong sample detection and long-term assay and lot consistency, giving you confidence in your research. (sigmaaldrich.com)
  • This article offers 4 popular ELISA protocols: Sandwich ELISA protocol, Phosphorylation Assay Procedure, EIA Assay Procedure, & Cell-based Assay Procedure. (sigmaaldrich.com)
  • Current methods for detecting BoNT include a mouse cause human botulism (A, B, E, and F). We developed a bioassay (2-4) and an enzyme-linked immunosorbent assay rapid, mass spectrometry-based method (Endopep-MS) to (ELISA) (5,6). (cdc.gov)
  • Besides, Protein Disulfide Isomerase A3 (PDIA3) has been identified as an interactor of PSA, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PSA and recombinant human PDIA3. (genetex.com)
  • Functional ELISA analysis of GTX00222-pro Human NPEPPS protein which can bind immobilized PDIA3 protein. (genetex.com)
  • Factor XIIIa and factor XIIIb antigen levels can be quantified by means of enzyme-linked immunosorbent assay (ELISA). (medscape.com)
  • METHODS: The ability of sera from animals immunised with different VAR2CSA constructs to inhibit IE binding to CSA was investigated in three in vitro assays using 96-well plates, petri dishes, capillary flow and an ex vivo placental perfusion assay. (ku.dk)
  • There was an overall concordance in the capacity of antibody-mediated inhibition, when comparing the in vitro assays with the perfusion assay, which more closely represents in vivo conditions. (ku.dk)
  • Hanes, J. & Plückthun, A. In vitro selection and evolution of functional proteins by using ribosome display. (nature.com)
  • With INDIGO's in vitro drug discovery assays, save resources and energy by identifying and characterizing adverse effects and potential risks before your drug candidate progresses further in the drug development process. (indigobiosciences.com)
  • Their self-renewing potential was investigated by colony forming cells assays and in vitro organotypic culture models. (who.int)
  • To monitor adenovirus-mediated wild-type p53 gene transfer, we have used a quantitative assay which tests the ability of human p53 to activate transcription in yeast. (elsevierpure.com)
  • Selective amplification of cellular and viral p53 transcripts followed by quantitative assessment of mutant p53 content with the assay permits measurement of the wild-type p53 transduction efficiency into SF-188, U251MG and HUG31 glioblastoma cells. (elsevierpure.com)
  • Building on recent studies suggesting that passenger mutations may "weakly affect tumor cell fitness by promoting or inhibiting tumor growth," the authors used a quantitative sequencing strategy known as FunSeq2 to score the predicted functional effects of somatic mutations present in 2,548 PCAWG tumor samples. (genomeweb.com)
  • A quantitative assay is required to confirm the diagnosis of factor XIII deficiency. (medscape.com)
  • The enzyme-linked immunosorbent spot (ELISpot ) assay enables visualization of multiple secretory products from a single responding cell. (sigmaaldrich.com)
  • Techniques for measuring protein S antigen include enzyme-linked immunosorbent assays (ELISAs) and radioimmunoassays. (medscape.com)
  • Thus, HERA is a novel cellular assay that can be used to predict how FcRn-binding proteins are rescued from intracellular degradation. (nature.com)
  • The cancer assays to be tested use DNA analysis and antibodies to specific proteins as well as functional assays for proteins to attempt to identify bladder tumor presence. (centerwatch.com)
  • Total Antioxidant Capacity Assay Kit ab65329 can measure either the combination of both small molecule antioxidants and proteins, or small molecules alone in the presence of our proprietary Protein Mask. (abcam.com)
  • Typically performed in multi-well microtiter plates, ELISAs are a molecular biology assay commonly used for the detection and quantification of diverse molecules, including peptides, proteins, and antibodies. (sigmaaldrich.com)
  • Split-complementation Endosomal Escape (SEE) provides the first direct assay visualizing true cytoplasmic-delivery of proteins at biologically relevant concentrations. (bvsalud.org)
  • Proximity ligation assay (in situ PLA) is a technology that extends the capabilities of traditional immunoassays to include direct detection of proteins, protein interactions, extracellular vesicles and post translational modifications with high specificity and sensitivity. (wikipedia.org)
  • Leveraging our expertise in screening and receive robust data from high-throughput assays. (horizondiscovery.com)
  • The SEE assay has minimal background, is amenable to high-throughput processes, and adaptable to different transient and stable cell lines . (bvsalud.org)
  • BD Biosciences offers a complete suite of assays and reagents for visualizing key cellular events. (bdbiosciences.com)
  • Choose from a variety of BD Biosciences assays and reagents for visualizing cellular events such as apoptosis, cell proliferation and the cell cycle. (bdbiosciences.com)
  • The Starter Pack is a bundle of Assay Complete reagents needed for culturing the specified cell line. (discoverx.com)
  • Starter Packs are a convenient, pre-packaged collection of Assay Complete reagents, including the relevant cell culture kit, cell plating reagent, freezing reagent, thawing reagent, and cell detachment reagent for the culture of the 93-0987C3 cell line. (discoverx.com)
  • Each kit includes luciferase reporter cells, an assay plate, all required reagents (optimized culture media, positive-control agonist, and detection reagent), and a detailed Technical Manual. (indigobiosciences.com)
  • Each assay kit includes RTU cryopreserved cells, optimized assay reagents, plates, and a detailed assay protocol to ensure robust and reliable performance every time. (discoverx.com)
  • The BD ® Calcium Assay Kits enable the measurement of intracellular calcium changes caused by activation of G-protein coupled receptors or calcium channels, quickly and easily, by flow cytometry. (bdbiosciences.com)
  • In general, a DEL has been considered as a massive binding assay to identify physical binders for individual protein targets. (nature.com)
  • In the total antioxidant capacity assay protocol, the Cu2 + ion is converted to Cu + by both small molecule and protein antioxidants. (abcam.com)
  • This cutting-edge technology enables functional assays of all GPCRs regardless of coupled G-protein subtypes, making it a powerful tool in drug discovery enterprises. (tansobio.com)
  • Our core technology combines sensitive and versatile reporter cells, a comprehensive Gα protein cocktail, and a comprehensive human and mouse GPCR library to make possible functional assays of all GPCRs. (tansobio.com)
  • The core technology for our cell-based GPCR functional assay consists of sensitive and versatile test cells, a Gα protein cocktail, and an exhaustive human GPCR library. (tansobio.com)
  • The all-around Gα protein cocktail complements our proprietary assay cells and enables functional assays for any GPCR under a single protocol, regardless of the Gα protein the target receptor is coupled with. (tansobio.com)
  • GFP-complementation assay to detect functional CPP and protein delivery into living cells. (bvsalud.org)
  • Electrophoretic mobility shift assays detected allele-dependent nuclear protein binding in A549 cells for 8 of 21 variants. (cdc.gov)
  • T]he FunSeq tool assigns a molecular functional impact score to a mutation based on various features," the authors explained, such as "inter-species conservation, gain or break of transcription factor motifs, disruption of known enhancer-gene interactions, and centrality in the gene regulatory or protein-interaction network. (genomeweb.com)
  • Another variation of in situ PLA includes a multiplex PLA assay that makes it possible to visualize multiple protein complexes in parallel. (wikipedia.org)
  • Functional and structural characterization of human adrenoleukodystrophy protein (ALDP). (elifesciences.org)
  • After the VTE is adequately treated, protein C and free protein S functional and activity tests are ordered. (medscape.com)
  • The protein C and free protein S functional and activity tests measure the amount of each protein and evaluate whether they are performing their proper function in the body. (medscape.com)
  • Protein S assays can be either functional or immunological. (medscape.com)
  • For detecting free protein S, the C4bBP-bound protein S is removed from the assay by a pre-assay step involving precipitation of the C4bBP-bound protein S with polyethylene glycol and then using centrifugation to remove the precipitate. (medscape.com)
  • Functional assays measure only free (physiologically active) protein S. Functional assays are usually based on either the prothrombin time (PT) or the activated partial thromboplastin time (aPTT). (medscape.com)
  • With aPTT-based functional protein S assays, platelet-poor plasma is incubated at 37°C with the following: protein S-deficient plasma, phospholipid, a contact activator (eg, Kaolin), and either an excess of activated protein C or an activator of protein C .Typically, the incubation period is 1-4 minutes, after which calcium is added to initiate clotting. (medscape.com)
  • For PT-based functional protein S assays, the activated protein C cofactor activity of protein S is measured based on the degree of prolongation of a PT-based clotting time. (medscape.com)
  • Immunological assays measure total protein S, both free protein S and bound protein S. In rare circumstances, the level of immunological protein S is normal but some functional abnormality exists (ie, a true type 2 deficiency). (medscape.com)
  • Quickly gain insights into the mechanisms underlying the involvement of key receptors in various diseases with INDIGO's reporter assays. (indigobiosciences.com)
  • DNA constructs were cloned into a pGL3 promoter vector for luciferase gene reporter assays. (cdc.gov)
  • Tanso Biosciences has developed a high-performance functional assay to overcome the barriers in GPCR drug discovery. (tansobio.com)
  • Tanso Biosciences has developed a high-performance functional assay to overcome these barriers and make high-hanging GPCRs accessible to the pharmaceutical industry. (tansobio.com)
  • Review the oxidative stress marker and assay guide to learn about more assays for oxidative stress. (abcam.com)
  • Review the mitochondrial assay guide , or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader. (abcam.com)
  • Such assays can detect molecules of interest at the pg/mL level and are critical for both basic research and disease research application needs. (sigmaaldrich.com)
  • 1 With the high degree of cross-reactivity within the flaviviridae family, a diagnostic assay that can rapidly detect and differentiate between them, and determine new vs old infection, is of upmost importance and is a current area of focus for bioLytical. (prnewswire.com)
  • The homogeneous assay kit format allows you to detect agonists, antagonists, and allosteric modulators. (discoverx.com)
  • Hence, it is desirable to start with a biochemical assay followed by a cell-based assay to corroborate results in a physiological context (that is, to provide orthogonal validation). (genengnews.com)
  • Previous publications from this Urology Department have introduced the idea of a non-invasive, molecular-based assay for the detection and monitoring of bladder cancer (Levesque et al. (centerwatch.com)
  • We propose to assess the use of a multiple mutation-based assay, using DNA from exfoliated cells in the urine of patients, to establish the sensitivity and specificity in tumor detection compared to cystoscopy and cytology. (centerwatch.com)
  • The frequency of false positive results will determine the utility of the assay in the cancer detection arena. (centerwatch.com)
  • Sensitivity of an assay dictates the detection of the target within the dynamic range of the assay with the caveat that the lower limit of detection could vary marginally from batch to batch. (genengnews.com)
  • Both assay format and detection technology play major roles in sensitivity. (genengnews.com)
  • The dynamic range of an assay defines both the lower and upper limits of detection. (genengnews.com)
  • ELISAs that use direct detection methods require an immobilized antigen that is bound directly to the surface of an assay plate or indirectly by a capture antibody, followed by an antigen-specific primary antibody conjugated to an enzyme, and the detection substrate. (sigmaaldrich.com)
  • In addition to direct and indirect detection methods, capture or "sandwich" assays use an additional antigen-capturing antibody that is first attached to the microplate surface, followed by the use of both a primary and an enzyme-conjugated secondary antibody, similar to the indirect method previously described. (sigmaaldrich.com)
  • RICHMOND, BC , Aug. 19, 2016 /PRNewswire/ - bioLytical Laboratories, a world leader in rapid infectious disease tests, is pleased to announce that it has filed two patent applications with the U.S. Patent and Trademark Office relating to multiple color rapid detection assay systems. (prnewswire.com)
  • These technology breakthroughs can revolutionize point-of-care rapid assays for detection of acute infections including Zika. (prnewswire.com)
  • In situ proximity ligation assays (isPLA) has been applied to antibody validation in human tissues with various advantages over IHC, including increased detection specificity, decreased unspecific staining, and better localization. (wikipedia.org)
  • Furthermore, they have proved themselves in assays with other membrane receptors, such as receptor tyrosine kinases. (tansobio.com)
  • Evaluate your compounds on major receptors with INDIGO's reporter assay panels to understand their biological and toxicological effects better. (indigobiosciences.com)
  • Understand a compound's on- and off-target effects and liabilities on overlapping pathways with nuclear receptors functional classifications. (indigobiosciences.com)
  • We found that measurement of NO release by DAF-FM fluorescence in primary human aortic endothelial cells (HAEC) or in AT(2)R-transfected CHO cells is a reliable assay for the characterization of AT(2)R ligands. (mdc-berlin.de)
  • Phenotypic cellular assay models more accurately reflect the complex biology of disease, allows for multiparametric measurement of ex vivo or in vivo events, and enables the characterization of intractable target(s). (genengnews.com)
  • Our assay has yielded consistent readouts with results from existing methods and at high sensitivity and S/B ratio. (tansobio.com)
  • The approval was based on "comprehensive review of scientific evidence," including "comparisons of the products on an analytical level using an extensive battery of chemical and biological tests and biological assays that confirmed similarity in the structural and functional features of Wezlana and Stelara (including those known to impact safety and efficacy), and comparative human pharmacokinetic data, clinical immunogenicity data, and other clinical safety and effectiveness data," the FDA said. (medscape.com)
  • It is a multiple tumor biomarker DNA methylation assay for use with fresh frozen formalinfixed, paraffinembedded patient tumor samples. (azbio.org)
  • The lack of adequate and reliable assays has been a barrier to GPCR drug discovery. (tansobio.com)
  • Optimization of experimental assay design is crucial for the overall success of any drug discovery initiative. (genengnews.com)
  • eXpress Assay kits developed by Eurofins DiscoverX ® are ready-to-use (RTU) cell-based assay kits for functional mechanism of action (MOA) or binding assay for drug discovery target validation, screening and hit ID, and lead optimization applications. (discoverx.com)
  • Our proprietary assay cells are equipped with an artificial gene circuit that amplifies GPCR-induced intracellular signals, resulting in highly sensitive and specific results. (tansobio.com)
  • MegaCult™-C Collagen and Medium with Lipids kit includes medium and collagen solution for colony-forming unit (CFU) assays of human or mouse megakaryocyte progenitor cells (CFU-Mk). (stemcell.com)
  • A main challenge of NK cell therapy is that it requires a high amount of functional NK cells. (frontiersin.org)
  • Defining the paradigms and predictable end point(s) enables selection of the assay formats and technologies to be used for the study. (genengnews.com)
  • Troubleshoot and optimize ELISAs using this guide that includes solutions to some of the most common sources of problems for assay development. (sigmaaldrich.com)
  • The Von Willebrand Ristocetin Cofactor [vWF:RCo] assay measures the ability of a plasma sample to agglutinate platelets in the presence of the antibiotic Ristocetin. (practical-haemostasis.com)
  • Ristocetin Induced platelet agglutination (RIPA) is similar to the Ristocetin Cofactor Assay but RIPA measures platelet agglutination induced by Ristocetin-mediated VWF binding to the platelet Gp1b receptor but in this case the Ristocetin is added directly to the patient platelet rich plasma and there are no serial dilutions of the plasma sample. (practical-haemostasis.com)
  • The highest binding inhibition sensitivity was found in the 96-well and petri dish assays compared to the flow and perfusion assays where binding inhibition required higher antibody titers. (ku.dk)
  • CONCLUSIONS: The inhibitory capacity of antibodies is not easily translated between assays and the high sensitivity of the 96-well and petri dish assays stresses the need for comparing serial dilutions of serum. (ku.dk)
  • Technologically, luminescent and fluorescent modes offer greater sensitivity compared to absorbance assays. (genengnews.com)
  • We have developed a 3D (spheroid) functional assay to assess the sensitivity of two PARPis (niraparib and olaparib) in ascites-derived primary cell cultures (AsPCs) from HGSOC patients. (biomedcentral.com)
  • With the help of a functionally informative sequencing method called FunSeq2, the researchers identified non-driver, somatic mutations and their predicted functional impact in 2,548 tumors profiled for the Pan Cancer Analysis of Whole Genomes (PCAWG). (genomeweb.com)
  • The structure and functional studies of disease-causing mutations are solid and will appeal to the transporter and medical genetics communities. (elifesciences.org)
  • To evaluate the effect of splice-site mutations in two Lynch syndrome patients, we carried out a functional splicing assay using minigenes. (elsevierpure.com)
  • Furthermore, additional functional splicing assays using two different intronic mutations described in earlier studies revealed splicing alterations that were in complete agreement with the reports. (elsevierpure.com)
  • The application of this assay and the potential biomarkers with possible predictive significance to PARPis therapy of EOC patients now need testing in the setting of a clinical trial. (biomedcentral.com)
  • Measure and compare the activity of proteases with this non-specific protease activity assay using casein. (sigmaaldrich.com)
  • By eliminating lengthy, time-consuming, and expensive cell culture steps, eXpress assay kits enable you to screen your small molecule or biologic therapeutics and generate accurate, precise, and reproducible functional data in just a few hours. (discoverx.com)
  • Through the lens of genomic and functional assays. (lu.se)
  • RESULTS: The inter-assay variation was not uniform between assays and ranged from above ten-fold in the flow assay to two-fold in the perfusion assay. (ku.dk)
  • In the meantime, the Calgary-based company is raising $3.0 million to continue funding its efforts in the mineral-rich area and even teased investors in a recent conversation with Equities.com that more assay results should be coming in December. (equities.com)
  • The assays built upon surface chip sampling during the summer, where results included 3.4 meters grading 13.35% zinc, 2 meters at 15.58% zinc and 2 meters at 8.22% zinc from the Main zone, plus 2 meters grading 5.92% zinc from the Lerwick zone. (equities.com)
  • These results suggest that the yeast p53 functional assay may be a useful tool for monitoring p53 gene therapy. (elsevierpure.com)
  • Therefore, in patients with milder deficiencies or in patients who have recently received a transfusion, results of the clot stability assay may be normal. (medscape.com)
  • NEW YORK (Reuters Health) - Results from six insulin-like growth factor I (IGF-I) commercial immunoassays vary considerably and could result in different classification of patients with growth hormone disorders, according to a cross-sectional assay. (medscape.com)
  • Even when assays give different results, they would be expected to classify a given patient in the same way (high, normal or low values). (medscape.com)
  • These results confirm that, even when using normative values established in the same population of healthy subjects, IGF-I results obtained with different assays in a given individual, whether healthy (as in the VARIETE study), or having a growth hormone-related disorder (as in the present study), are sometimes very different, potentially leading to patient misclassification," the researchers conclude. (medscape.com)
  • It is not possible, according to the results of this study, to recommend one assay or the other," they note. (medscape.com)
  • IGF-I results should be therefore interpreted in an appropriate clinical context, and patient's monitoring during diagnostic work-up and therapeutic management should use the same IGF-I assay. (medscape.com)
  • Indeed, there is a need to increase precision of commercial IGF-I assays, as IGF-I results act as marker of disease and guide future changes in usually costly treatments. (medscape.com)
  • The factors that need to be considered include: selection of the most appropriate assay technologies, reading modalities, and experimental models (biochemical, cellular, or animal). (genengnews.com)
  • Biochemical assays are simpler to execute than their cellular counterparts and offer better consistency. (genengnews.com)
  • Trouble-shoot with INDIGO's large portfolio of ortholog assays to quickly determine if the response observed in laboratory animals is concordant with humans. (indigobiosciences.com)
  • 1.96), assays were relatively concordant for low values, weakly or poorly concordant for normal values, and variable for high values. (medscape.com)
  • From a practical point of view, very high levels or very low levels of IGF-I are generally concordant, whatever the assay which is used and classification of patients as having active acromegaly or severe growth hormone deficiency is generally similar. (medscape.com)
  • Included in this kit for performance of the activity assay are buffer, detergent, substrate, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay. (abcam.com)
  • Identify therapeutic agents with the highest selectivity and lowest potential for off-target and unwanted effects with INDIGO's cell-based receptor assays. (indigobiosciences.com)
  • A positive correlation between IE binding avidity and the level of binding after antibody inhibition in the petri dish assay indicate that high avidity IE binding is more difficult to inhibit. (ku.dk)
  • An assay that is based upon the binding of a monoclonal antibody to a VWF A1 domain epitope. (practical-haemostasis.com)
  • Complex IV Rodent Enzyme Activity Microplate Assay Kit (ab109911) is used to determine the activity of cytochrome c oxidase in a mouse sample with speed and simplicity. (abcam.com)
  • This lack of standard assays has hampered the development of new drugs targeting the AT(2)R. Therefore, we aimed at developing a reliable, technically easy assay for the determination of intrinsic activity of AT(2)R ligands, primarily for distinguishing between AT(2)R agonists and antagonists. (mdc-berlin.de)
  • The rate of Ristocetin induced agglutination is related to the concentration and functional activity of the plasma von Willebrand factor. (practical-haemostasis.com)
  • The method is similar to a factor assay and was for many years the only method available for measuring VWF activity [VWF:RCo]. (practical-haemostasis.com)
  • Use this assay as a standardized procedure to determine the activity of proteases for quality control. (sigmaaldrich.com)
  • Yet most current CPP activity assays are hampered by limitations in assessing uptake, including confounding effects of conjugated fluorophores or ligands , indirect read-outs requiring secondary processing, and difficulty in discriminating internalization from endosomally trapped cargo. (bvsalud.org)
  • RiboMed's brain cancer prognostic assay launched in the CLIA lab setting in April 2012, Hanna noted. (azbio.org)
  • Risk and nonrisk oligonucleotides were tested for binding of nuclear extracts from A549, BEAS-2B, and IMR-90 lung cell lines by using electrophoretic mobility shift assays. (cdc.gov)
  • Minimize assay development time and eliminate the need for cell culture. (discoverx.com)
  • Cell functional assays showed that Atezolizumab in combination with Bevacizumab inhibited the proliferation, migration, and invasion of cisplatin resistant ovarian cancer cell line A2780cis synergistically, which maybe associate with Bevacizumab suppressing the epithelial-mesenchymal transition (EMT) and PD-L1 expression by targeting STAT3. (spierarchitecturalarts.com)
  • Objective: The aim of this study was to identify DA-associated functional genetic variants through next-generation sequencing (NGS), bioinformatics, and functional assays. (cdc.gov)
  • Therefore, functional splicing assay is helpful for evaluating the effects of genetic variants on splicing. (elsevierpure.com)
  • The UG website uses functional and anonymous analytics cookies. (rug.nl)
  • Wash-based assays can be used to circumvent interference caused by highly complex sample types, as molecules within the matrix that contribute to interference are washed away. (genengnews.com)
  • Do I need any special equipment to utilize INDIGO's assay kits? (indigobiosciences.com)
  • Both assays are available as commercial kits. (medscape.com)
  • Dr. Philippe Chanson from Hopital de Bicetre in Le Kremlin-Bicetre, France, and colleagues measured IGF-I with the same six assay kits in 102 patients with acromegaly (n=56), growth hormone deficiency (n=14) or other pituitary or endocrine disorders tested for suspected growth hormone disorder (n=32). (medscape.com)
  • The purpose of this study was to evaluate the robustness and comparability of binding inhibition assays used in the clinical development of placental malaria vaccines. (ku.dk)