Puromycin is a reversible inhibitor of dipeptidyl-peptidase II (serine peptidase) and cytosol alanyl aminopeptidase (metallopeptidase). (wikipedia.org)
The mechanism of inhibition is not well understood, however puromycin can be used to distinguish between aminopeptidase M (active) and cytosol alanyl aminopeptidase (inhibited by puromycin). (wikipedia.org)
Specific puromycin-sensitive aminopeptidase (PSA) inhibitors with a 3-(halogenated phenyl)-2,4(1H,3H)-quinazolinedione skeleton were prepared and their structure-activity relationships were investigated. (elsevierpure.com)
Puromycin Sensitive Aminopeptidase (PSA) also known as cytosol alanyl aminopeptidase or alanine aminopeptidase (AAP) is used as a biomarker to detect damage to the kidneys, and that may be used to help diagnose certain kidney disorders. (genetex.com)
This gene encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. (genetex.com)
In contrast puromycin-sensitive aminopeptidase (PsaA) also bound to NumA1 but this association was limited to the nucleoplasm. (utoronto.ca)
Antibiotic3
Puromycin is an antibiotic protein synthesis inhibitor which causes premature chain termination during translation. (wikipedia.org)
Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that causes premature chain termination during translation taking place in the ribosome. (wikipedia.org)
Puromycin In addition to delivering the hairpin, the lentivirus makes the cells resistant to the antibiotic puromycin. (broadinstitute.org)
The kit is composed of a lentiviral packaging plasmid mix (LV PacMix), control lentiviral plasmids carrying EGFP and Puromycin resistant gene, and Polybrene , and can be used for efficient packaging and purification of high titer lentiviruses for use in vitro and in vivo experiments. (ubigene.us)
Oocytes1
However, when combined with activating agents ionomycin and puromycin, parthenogenetic activation of MII oocytes allows the second polar body to extrude. (pharmaceutical-technology.com)
Plates containing puromycin are stable for 1 month when stored at 4 °C.[citation needed] Puromycin resistance in yeast can also be conferred through expression of the puromycin N-acetyl-transferase (pac) gene. (wikipedia.org)
Deletion of the gene encoding the multidrug efflux pump Pdr5 sensitizes cells to puromycin. (wikipedia.org)
Stable, long-term expression of your Custom Gene for your cell line of interest with custom selection marker (Puromycin, Blasticidin or Neomycin). (virostem.com)
10/21/2011 · What is the application of gene cloning in The chimeric DNA or rDNA formed by cloning is stable and can be used to propagate and sequence the DNA. (fullsportpress.net)
Gene Cloning and Its Medical Uses Qasim Munye 18th September 2014 Respond In its simplest form, for example in the treatment of single gene (monogenic) disorders, gene therapy can be considered the replacement of a defective gene in an individual with a functional "wild type" version of the gene to reverse the pathology. (fullsportpress.net)
We also extend upon reporter assays involving C-terminally truncated AR forms to show occupancy at an AR regulated promoter and transcriptional activation of an AR regulated gene. (axlinhibitor.com)
ShRNA sequence1
Briey, two single-stranded oligonucleotides are annealed to form a double-stranded DNA sequence containing the shRNA sequence followed by a Pol III terminator (consisting of a stretch of four or more thymidines), as well as 5'- and 3'-end "sticky" overhangs that are complementary to those generated in the targeting vector following restriction digestion with BglII and SalI (Figures 3E and 4C). (berterolab.com)
Peptides2
Intracellular delivery of multiple functional peptides forms a basis for potent, non-invasive mode of delivery, providing distinctive therapeutic advantages. (aacrjournals.org)
Cycloheximide or puromycin inhibition of general protein synthesis does not lead to NN formation. (silverchair.com)
Stable2
Puromycin is stable for one year as solution when stored at -20 °C. The recommended dose as a selection agent in cell cultures is within a range of 1-10 μg/ml, although it can be toxic to eukaryotic cells at concentrations as low as 1 μg/ml. (wikipedia.org)
The encoded protein forms a stable complex with PYK2 in vivo. (novusbio.com)
Protein3
In this reaction, a puromycin molecule is chemically attached to the end of an mRNA template, which is then translated into protein. (wikipedia.org)
As puromycin inhibits protein synthesis in eukaryotic cells, researchers were able to show that injections of this drug will result in both short-term as well as long-term memory loss in mice. (wikipedia.org)
Ubigene Lentivirus Packaging Kit uses a third-generation packaging system in which the 3'LTR of the genome is mutated to form self inactivation (SIN), whereby the viral genome, when integrated into the cell genome, does not produce new progeny virus and therefore has a relatively good safety profile. (ubigene.us)
Mice1
This disk membrane deficiency is one of the most severe among the retinal degeneration models and is comparable to rhodopsin knock-out mice that are also incapable of forming disks. (jneurosci.org)
Selective2
Puromycin is selective for either prokaryotes or eukaryotes. (wikipedia.org)
Puromycin is used in cell biology as a selective agent in cell culture systems. (wikipedia.org)
Cell1
Lethal concentrations of puromycin are much higher for strains of Saccharomyces cerevisiae than mammalian cell lines. (wikipedia.org)
Days1
Puromycin acts quickly and can kill up to 99% of nonresistant cells within 2 days. (wikipedia.org)
Cells2
Thus, one can measure the fraction of cells infected by infecting two wells with lentiviruses for the same hairpin, treat only one of the wells with puromycin, and count the number of surviving cells in each of the two wells. (broadinstitute.org)
Aberrantly activated Wnt signaling in KRAS-mutant cells was demonstrated to transcriptionally repress miR-139-5p through TCF4, forming a miR-139-5p/Wnt signaling double-negative feedback loop. (thno.org)
Translation1
Timed actinomycin D and puromycin treatments show that transcription and translation are required for comb formation and suggest that the segregated material might be necessary for activation of the appropriate genes. (illinois.edu)
Liquid1
Lenviruses are provided frozen or in liquid form depending on your needs. (virostem.com)
Selection2
But use of puromycin for E. coli selection requires precise pH adjustment and also depends on which strain is selected. (wikipedia.org)
For hassle-free selection and optimum results the use of specially modified puromycin is possible. (wikipedia.org)
Found1
These nucleic acid binding domains are predominantly found in elongation factor P, where they adopt an OB-fold, with five beta-strands forming a beta-barrel in a Greek-key topology ( PUBMED:15210970 ). (embl-heidelberg.de)
Document1
No part of this document may be reproduced in any form without prior permission in writing. (multispaninc.com)
Link1
The puromycin can then form a covalent link to the growing peptide chain allowing the mRNA to be physically linked to its translational product. (wikipedia.org)
Expression1
Only one VSG is transcribed at a time from one of 15 bloodstream-form expression sites (BESs). (asm.org)
Feedback1
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. (fishersci.com)